CN213012893U

CN213012893U - Nucleic acid extraction system

Info

Publication number: CN213012893U
Application number: CN202020917809.1U
Authority: CN
Inventors: 刘和录; 朱美玲; 窦宇红; 杜纪坤; 张大威
Original assignee: Shenzhen Integrated Traditional Chinese And Western Medicine Hospital
Current assignee: Shenzhen Integrated Traditional Chinese And Western Medicine Hospital
Priority date: 2020-05-26
Filing date: 2020-05-26
Publication date: 2021-04-20
Anticipated expiration: 2030-05-26

Abstract

The application provides a nucleic acid extraction system, which comprises a kit, a reagent conveying device, a nucleic acid extraction condition control device and a magnetic bead transfer device; the kit is respectively stored with a plurality of extraction reagents, the nucleic acid extraction condition control device is used for providing rapid heating control, oscillation mixing control and a plurality of groups of extraction tube groups, each extraction tube group comprises a plurality of extraction tubes which are sequentially arranged along a first direction, a sample reagent is stored in one extraction tube, the reagent conveying device is used for conveying various extraction reagents in the kit into the extraction tubes, and the magnetic bead transfer device is used for transferring magnetic beads after reaction of the extraction tubes to the next extraction tube according to a preset sequence; the volume of each extraction tube was 6 ML. By setting the volume of the extraction tube to be larger than that of a common extraction tube, more sample reagents can be stored in the extraction tube, the more sample reagents are, the more nucleic acid can be extracted, the concentration of the extracted nucleic acid is increased, and the detection precision of the nucleic acid is improved.

Description

Nucleic acid extraction system

Technical Field

The application belongs to the technical field of nucleic acid extraction, and particularly relates to a nucleic acid extraction system.

Background

Along with the popularization of gene detection, personalized drug delivery, prenatal diagnosis and the like, the traditional DNA extraction method is more and more obviously limited today when high flux and automation are pursued in various fields of the biological industry. Because the magnetic bead method for extracting nucleic acid can realize automatic extraction and large-scale operation, and has simple operation and short time, the magnetic bead method for extracting nucleic acid is more and more emphasized.

Generally, the extraction steps for extracting nucleic acid by the magnetic bead method mainly comprise: (1) cracking; (2) combining; (3) rinsing; (4) and (4) eluting. Specifically, the DNA/RNA is released from cells or tissues under the action of a lysis solution, and the surface-modified superparamagnetic silica nano magnetic beads are specifically combined with the released DNA/RNA to form a nucleic acid-magnetic bead compound. Adding a rinsing liquid into the nucleic acid-magnetic bead compound, washing off impurities such as non-specifically adsorbed protein and polysaccharide, separating the nucleic acid-magnetic bead compound with the impurities removed from the magnetic bead in an eluent, and finally obtaining a nucleic acid substance to be extracted. If the steps of cracking, combining, rinsing and eluting are not mixed uniformly in the nucleic acid extraction process, the reagent is not fully contacted with the sample, the intracellular nucleic acid is not completely released, the magnetic beads are easy to settle, and the extraction efficiency is greatly reduced.

When the concentration of the nucleic acid in the sample is extremely small, the amount of the nucleic acid is below its lower detection limit. Meanwhile, when the concentration of nucleic acid in a sample is extremely low, it is possible that nucleic acid cannot be detected.

SUMMERY OF THE UTILITY MODEL

The embodiment of the application aims to provide a nucleic acid extraction system to solve the technical problem that the concentration of nucleic acid is too low in the prior art.

In order to achieve the purpose, the technical scheme adopted by the application is as follows: providing a nucleic acid extraction system, which comprises a kit, a reagent conveying device, a nucleic acid extraction condition control device and a magnetic bead transfer device; the kit is characterized in that a plurality of extraction reagents are respectively stored on the kit, the nucleic acid extraction condition control device is used for providing rapid heating control, oscillation mixing control and a plurality of groups of extraction tube groups, each extraction tube group comprises a plurality of extraction tubes which are sequentially arranged along a first direction, a sample reagent is stored in one of the extraction tubes, the reagent conveying device is used for conveying each extraction reagent in the kit to each extraction tube, and the magnetic bead transferring device is used for transferring magnetic beads after reaction of the extraction tubes to the next extraction tube according to a preset sequence; wherein the volume of each extraction tube is 6 ML.

Optionally, the extraction tube comprises a large diameter section and a small diameter section which are connected with each other, the large diameter section is close to the opening end of the extraction tube, and the small diameter section is close to the bottom end of the extraction tube; the height of major diameter section is greater than the height of path section, and the internal diameter of major diameter section is greater than the internal diameter of path section.

Optionally, the cross section of the large-diameter section is square, and the area of the cross section of the large-diameter section is constant along the height direction; the cross section of the small-diameter section is circular, and the cross section area of the small-diameter section is gradually reduced from the top end to the bottom end.

Optionally, the reagent box is partitioned by a partition plate to form a plurality of first reagent compartments arranged along the first direction and at least one second reagent compartment distributed along the second direction, the first direction is perpendicular to the second direction, and the extraction reagents in the first reagent compartments are delivered to the extraction tubes in each group of the extraction tube sets in a one-to-one correspondence manner.

Optionally, the nucleic acid extraction system further comprises a base frame, the reagent conveying device comprises a first horizontal conveying mechanism mounted on the base frame, a second horizontal conveying mechanism mounted on the first horizontal conveying mechanism, a first lifting mechanism mounted on the second horizontal conveying mechanism, and a nozzle rack mounted on the first lifting mechanism, the nozzle rack is used for mounting a nozzle and driving the nozzle to work; the first horizontal conveying mechanism is used for driving the suction nozzle frame to move along the first direction, and the second horizontal conveying mechanism is used for driving the suction nozzle frame to move along the second direction.

Optionally, the nucleic acid extraction system further comprises a nozzle supplying area and a nozzle removing area, the nozzle supplying area and the nozzle removing area are respectively arranged on the base frame, the nozzle supplying area is used for supplying nozzles to the nozzle rack, and the nozzle removing area is used for removing the used nozzles from the nozzle rack.

Optionally, be equipped with on taking off the suction nozzle district and take off the mouth frame, it has seted up the open slot on the mouth frame to take off, the open slot is used for blocking the suction nozzle so that the suction nozzle is followed the suction nozzle frame takes off, and blocks the back, the open slot with suction nozzle interference fit.

Optionally, the magnetic bead transfer device includes a third horizontal conveying mechanism disposed on the base frame, a second lifting mechanism and a third lifting mechanism mounted on the third horizontal conveying mechanism through the same mounting seat, a magnetic sleeve frame disposed on the second lifting mechanism and capable of performing lifting motion under the driving of the second lifting mechanism, a plurality of magnetic sleeves disposed on the magnetic sleeve frame, a magnetic rod frame disposed on the third lifting mechanism and capable of performing lifting motion under the driving of the third lifting mechanism, and a plurality of magnetic rods disposed on the magnetic rod frame, wherein each of the magnetic sleeves and each of the magnetic rods are disposed in one-to-one correspondence along the vertical direction.

Optionally, the nucleic acid extraction condition control means is provided with at least two rows of extraction tube groups, each row of the extraction tube groups being arranged at intervals in the first direction, and each of the extraction tube groups in each row of the extraction tube groups being arranged at intervals in the second direction; the magnetic sleeve rack is provided with at least two rows of magnetic sleeves, and the magnetic rod rack is provided with at least two rows of magnetic rods.

Optionally, at least two groups of extraction tube sets are in an integral connection structure along the second direction.

The nucleic acid extraction system provided by the application has the beneficial effects that: compared with the prior art, the nucleic acid extraction system that this application provided sets up to 6ML through the volume with the extraction tube, and the volume through with the extraction tube sets up to be bigger than ordinary extraction tube's volume, can store more sample reagent like this in the extraction tube, and the sample reagent is more, and the nucleic acid quantity of extracting is just more, and then the concentration of extracting back nucleic acid will increase to the detection of the nucleic acid of being convenient for, and then improve the detection precision of nucleic acid.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise.

FIG. 1 is a schematic perspective view of a nucleic acid extraction system provided in an embodiment of the present application;

FIG. 2 is a schematic view showing the construction of the base plate, the nucleic acid extracting condition controlling apparatus, the reagent cartridge, the nozzle supply area and the nozzle removal area in FIG. 1;

FIG. 3 is a schematic view of the extraction tube set of FIG. 2;

FIG. 4 is a schematic structural diagram of the reagent delivery device and the magnetic bead transfer device in FIG. 2;

FIG. 5 is a schematic structural view of the reagent delivery device of FIG. 4;

FIG. 6 is a schematic structural diagram of the magnetic bead transfer device in FIG. 5.

Wherein, in the figures, the respective reference numerals:

10-a base frame; 20-a kit; 30-a reagent delivery device; 40-nucleic acid extraction condition control means; 50-magnetic bead transfer device; 60-a suction nozzle supply area; 70-a suction nozzle removal area; 11-a base plate; 12-a cross beam; 13-a stringer; 14-a first mounting plate; 21-first reagent grid; 22-second reagent grid; 31-a first horizontal conveyance mechanism; 32-a second horizontal conveyance mechanism; 33-a first lifting mechanism; 34-a nozzle holder; 35-a suction nozzle sleeve; 41-extraction tube group; 51-a third horizontal conveyance mechanism; 52-a second lifting mechanism; 53-a third lifting mechanism; 54-magnetic sleeve frame; 55-magnetic sleeve; 56-magnetic rod rack; 57-magnetic bar; 58-a mounting frame; 59-a suction nozzle; 61-a nozzle plate; 71-a mouth-off frame; 72-open slots; 411-extraction tube; 610-mounting holes; 4111-a large diameter section; 4112-small diameter section; x-a first direction; y-second direction.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects to be solved by the present application clearer, the present application is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.

It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, as used herein, refer to an orientation or positional relationship indicated in the drawings that is solely for the purpose of facilitating the description and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the present application.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.

Referring to FIG. 1 and FIG. 2, a nucleic acid extraction system according to an embodiment of the present application will now be described. The nucleic acid extraction system is used for extracting nucleic acid by a magnetic bead method.

The nucleic acid extraction system includes a reagent kit 20, a reagent transfer device 30, a nucleic acid extraction condition control device 40, and a magnetic bead transfer device 50.

Six extraction reagents are stored in the reagent cartridge 20. Specifically, the extraction reagent is lysis solution, binding solution, rinsing solution or eluent, the sample reagent is cell or tissue solution, the lysis solution is used for releasing (nucleic acid) DNA/RNA in the cell or tissue solution through lysis, the binding solution is used for binding magnetic beads and nucleic acid, the rinsing solution is used for washing impurities such as nonspecifically adsorbed proteins and polysaccharides in the nucleic acid, the nucleic acid can be washed only after 1-3 times of washing, and the eluent is used for eluting the nucleic acid from the magnetic beads to complete the extraction of the nucleic acid. In this embodiment, the six reagents are lysis solution, capping solution, binding solution, two rinsing solutions and eluent, respectively, where the lysis solution is reagent 1, the capping solution is reagent 5, the binding solution is reagent 6, the two rinsing solutions are reagent 2 and reagent 3, respectively, and the eluent is reagent 4.

The nucleic acid extraction condition control device 40 is used to provide rapid temperature rise control, shaking and mixing control, and a plurality of extraction tube groups 41. The extraction tube set 41 includes a plurality of extraction tubes 411 arranged in sequence along the first direction X, wherein one of the extraction tubes 411 stores a sample reagent. Specifically, the extraction tube set 41 includes four extraction tubes 411, the first direction X is the left-right direction in fig. 1, wherein a sample reagent is stored in the extraction tube 411 located at the leftmost side, the reagents 1 to 4 are respectively arranged corresponding to the four extraction tubes 411, the reagents 5 and 6 both correspond to the leftmost extraction tube 411, that is, a set of extraction tubes 411 can implement all steps of nucleic acid extraction, and the number of the extraction tubes 411 in the extraction tube set 41 is related to the number of times of rinsing, when only rinsing is performed once, the number of the extraction tubes 411 is three, when rinsing is performed three times, the number of the extraction tubes 411 is five, and this is not limited uniquely here. Here, it should be noted that the rapid temperature rise control and the shaking and mixing control are both used to provide temperature and vibration to the reaction in each extraction pipe 411 so as to promote the reaction in the extraction pipe 411.

The reagent conveying device 30 is used for conveying each extraction reagent in the reagent kit 20 to each extraction tube 411, and the magnetic bead transferring device 50 is used for conveying the magnetic beads reacted by the extraction tubes 411 to the next extraction tube 411 according to a preset sequence. The specific working process is as follows: firstly, conveying the reagents 1 to 4 to four extraction tubes 411 respectively through a reagent conveying device 30, and arranging the reagents in a one-to-one correspondence manner along a first direction X, then conveying the reagent 5 to the leftmost extraction tube 411 through the reagent conveying device 30, and performing rapid temperature rise control and oscillation and uniform mixing control on the extraction tubes 411 to promote the uniform reaction of the sample reagent, the reagent 1 and the reagent 5; then, the reagent 6 is transferred to the extraction tube 411 after the reaction by the reagent transfer device 30, and magnetic beads are supplied to the extraction tube 411 by the magnetic bead transfer device 50, so that the magnetic beads are bound to the nucleic acids. Then, the magnetic beads and the nucleic acids in the extraction tube 411 are sequentially transferred to the second extraction tube 411 and the third extraction tube 411 by the magnetic bead transfer device 50 for rinsing, and the magnetic beads and the nucleic acids are transferred to the fourth extraction tube 411 by the magnetic bead transfer device 50 for eluting the magnetic beads, and finally the magnetic beads are transferred by the magnetic bead transfer device 50.

The volume of each extraction tube 411 is 6 ML. Specifically, in this embodiment, 500. mu.l of the sample reagent can be stored in the extraction tube 411, and the volume of the nucleic acid after the final extraction is 25. mu.l, so that the nucleic acid can be concentrated 20 times, and the concentration ratio thereof can be increased.

Compared with the prior art, the nucleic acid extraction system 100 provided by the application has the advantages that the volume of the extraction tube 411 is set to be 6ML, the volume of the extraction tube 411 is set to be larger than that of the common extraction tube 411, so that more sample reagents can be stored in the extraction tube 411, the more sample reagents are used, the more extracted nucleic acid is used, the concentration of the extracted nucleic acid is increased, the detection of the nucleic acid is facilitated, and the detection precision of the nucleic acid is improved.

In this embodiment, please refer to fig. 3, the extraction tube 411 includes a large diameter section 4111 and a small diameter section 4112 connected to each other, the large diameter section 4111 is close to the open end of the extraction tube 411, the small diameter section 4112 is close to the bottom end of the extraction tube 411, that is, the large diameter section 4111 extends from the open end to the small diameter section 4112, the small diameter section 4112 extends from the connection point with the large diameter section 4111 to the bottom end, and the large diameter section 4111 and the small diameter section 4112 are integrally connected, that is, the whole extraction tube 411 is integrally formed. In addition, the height of the large diameter section 4111 is greater than the height of the small diameter section 4112, the inner diameter of the large diameter section 4111 is greater than the inner diameter of the small diameter section 4112, and the volume of the large diameter section 4111 is greater than the volume of the small diameter section 4112. This embodiment is through falling into big footpath section 4111 and path section 4112 with extraction tube 411, like this, can hold the great sample reagent of volume and extract reagent through big footpath section 4111's big volume, and when sample reagent and the volume of extracting reagent be too little, because path section 4112's internal diameter is little, then the too little solution of volume also can present certain height in path section 4112, subsequent bar magnet of so being convenient for rocks from top to bottom in extraction tube 411, and then makes this extraction tube 411's accommodation wider.

In this embodiment, please refer to fig. 3, a cross section of the large diameter section 4111 is square, and a cross sectional area of the large diameter section 4111 is constant along a height direction. The cross section of path section 4112 is circular, and the cross sectional area of path section 4112 reduces gradually from the top to the bottom, is also path section 4112 to be coniform, so, is close to the bottom more, the more fast the high rising of solution, even easy very little solution is poured into all can rise great height, the magnetic rod of being convenient for rocks from top to bottom for this extraction tube 411's accommodation is wider. It should be understood that, in other embodiments of the present application, the cross section of the large diameter section 4111 may also be a circle, and the cross section of the small diameter section 4112 may also be a square, which is not limited herein.

In this embodiment, referring to fig. 2, the reagent kit 20 is partitioned by a partition plate to form four first reagent compartments 21 and two second reagent compartments 22, the four first reagent compartments 21 are arranged at intervals along a first direction X, the two second reagent compartments 22 are arranged at intervals along a second direction Y, the first direction X is perpendicular to the second direction Y, and the second direction Y is a front-back direction in fig. 2. The extraction reagents in the four first reagent compartments 21 are fed into the four extraction tubes 411 in each set of the extraction tubing set 41 in a one-to-one correspondence, and the extraction reagents in the two second reagent compartments 22 are fed into the leftmost extraction tube 411 in each set of the extraction tubing set 41 in a corresponding manner. Specifically, the four first reagent compartments 21 are respectively used for storing the reagent 1, the reagent 2, the reagent 3 and the reagent 4 from left to right, and the two second reagent compartments 22 are respectively used for storing the reagent 5 and the reagent 6. Likewise, the number of the first reagent compartments 21 varies with the number of the extraction tubes 411 in each set of the extraction tube group 41, i.e., the number of the first reagent compartments 21 may be three or five.

Referring to fig. 2, the length of the second reagent compartments 22 along the first direction X is the total length of the four first reagent compartments 21 along the first direction X, such that the whole reagent kit 20 is square.

In this embodiment, referring to fig. 1, 4 and 5, the nucleic acid extracting system further includes a base frame 10, the reagent conveying device 30 includes a first horizontal conveying mechanism 31, a second horizontal conveying mechanism 32, a first lifting mechanism 33 and a nozzle holder 34, the first horizontal conveying mechanism 31 is mounted on the base frame 10, the second horizontal conveying mechanism 32 is mounted on the first horizontal conveying mechanism 31, the first lifting mechanism 33 is mounted on the second horizontal conveying mechanism 32, the nozzle holder 34 is mounted on the first lifting mechanism 33, the nozzle holder 34 is used for mounting a nozzle 59 and driving the nozzle 59 to work, the first horizontal conveying mechanism 31 is used for driving the nozzle holder 34 to move along a first direction X, and the second horizontal conveying mechanism 32 is used for driving the nozzle holder 34 to move along a second direction Y.

Specifically, referring to fig. 1, the base frame 10 is a frame structure formed by assembling a bottom plate 11, four cross beams 12 and four longitudinal beams 13. The middle of the left and right beams 12 is connected with a first mounting plate 14, the first mounting plate 14 extends along a first direction X and is horizontally arranged at the center of the top of the whole base frame 10, the nucleic acid extraction condition control device 40 is arranged at the middle of the bottom plate 11, the reagent kit 20 is arranged on the bottom plate 11 and is positioned at the left side of the nucleic acid extraction condition control device 40, the first horizontal conveying mechanism 31 is arranged at the lower side of the first mounting plate 14 and is used for conveying the second horizontal conveying mechanism 32, the first lifting mechanism 33 and the suction nozzle frame 34 in the first direction X repeatedly, the second horizontal conveying mechanism 32 is used for conveying the first lifting mechanism 33 and the suction nozzle frame 34 in the second direction Y repeatedly, and the first lifting mechanism 33 is used for conveying the suction nozzle frame 34 in the vertical direction.

In this embodiment, referring to FIG. 2, the nucleic acid extracting system further includes a nozzle supply area 60 and a nozzle removal area 70, the nozzle supply area 60 and the nozzle removal area 70 are respectively configured on the base frame 10, the nozzle supply area 60 is used for supplying the nozzles 59 to the nozzle holder 34, and the nozzle removal area 70 is used for removing the used nozzles 59 from the nozzle holder 34. Specifically, the suction supply nozzle region 60 and the suction removal nozzle region 70 are both provided on the left side of the nucleic acid extraction condition control apparatus 40, and the suction removal nozzle region 70, the suction supply nozzle region 60, and the reagent cartridge 20 are arranged in this order in the second direction Y. The present embodiment can remove and replace the used suction nozzle 59 by providing the suction nozzle supply area 60 and the suction nozzle removal area 70, thereby preventing various reagents from being mixed by the suction nozzle 59 and improving the extraction purity.

Specifically, referring to fig. 2 and fig. 5, a nozzle plate 61 is disposed on the nozzle region 60, six mounting holes 610 are disposed on the nozzle plate 61, and corresponding to the layout of the reagent kit 20, four of the mounting holes 610 are spaced apart from each other along the first direction X, and the other two mounting holes 610 are disposed along the second direction Y. Each mounting hole 610 is provided with a suction nozzle 59, the nozzle holder 34 is correspondingly provided with four nozzle sleeves 35, and when the nozzle holder 34 is moved above the suction nozzle 59, the nozzle holder 34 moves downwards and enables the openings of the suction nozzles 59 to be sleeved on the nozzle sleeves 35, so that the suction nozzles 59 are mounted on the nozzle holder 34. Four motors are also provided on the nozzle holder 34 for controlling the respective nozzles 59 to suck and drip liquid.

In the embodiment, referring to fig. 2, a nozzle releasing frame 71 is disposed on the nozzle releasing area 70, an opening slot 72 is disposed on the nozzle releasing frame 71, the opening slot 72 is U-shaped, the aperture of the opening slot 72 is smaller than the aperture of the suction nozzle 59, the opening slot 72 is used for clamping the suction nozzle 59 to release the suction nozzle 59 from the nozzle frame 34, and after the suction nozzle is clamped, the opening slot 72 and the suction nozzle 59 are in interference fit. Specifically, the open slot 72 is horizontally arranged, and the opening of the open slot 72 faces the nozzle supply area 60, when in operation, the first horizontal conveying mechanism 31, the second horizontal conveying mechanism 32 and the first lifting mechanism 33 move the nozzle holder 34 and the nozzle 59 to the front side of the open slot 72, and forcibly clamp the nozzle 59 into the open slot 72 from the opening of the open slot 72, because the nozzle 59 is made of a flexible material, and the nozzle removal holder 71 is made of a hard material, when the aperture of the open slot 72 is slightly smaller than the aperture of the nozzle 59, the nozzle 59 can be clamped into the open slot 72 under the driving of the second horizontal conveying mechanism 32, and when the nozzle holder 34 is driven by the first lifting mechanism 33 to move upwards, the nozzle 59 will be separated from the nozzle sleeve 35 and remain in the nozzle removal area 70.

In this embodiment, referring to fig. 4 and fig. 6, the magnetic bead transferring apparatus 50 includes a third horizontal conveying mechanism 51, a second lifting mechanism 52, a third lifting mechanism 53, a magnetic sleeve frame 54, a plurality of magnetic sleeves 55, a magnetic rod frame 56, and a plurality of magnetic rods 57.

The third horizontal transfer mechanism 51 is mounted on the base frame 10, and the second lifting mechanism 52 and the third lifting mechanism 53 are mounted on the first horizontal transfer mechanism 31 through the same mounting base 58 and spaced from each other. Specifically, the second lifting mechanism 52 is used for generating lifting movement, the magnetic sleeve frame 54 is arranged on the second lifting mechanism 52 and can be driven by the second lifting mechanism 52 to do lifting movement, and each magnetic sleeve 55 is arranged on the magnetic sleeve frame 54. The third lifting mechanism 53 is used for generating lifting movement, the magnetic bar frame 56 is arranged on the third lifting mechanism 53 and can be driven by the third lifting mechanism 53 to do lifting movement, each magnetic bar 57 is arranged on the magnetic bar frame 56, and each magnetic sleeve 55 and each magnetic bar 57 are arranged in one-to-one correspondence along the vertical direction. In an initial state, the second lifting mechanism 52 and the third lifting mechanism 53 respectively drive the magnetic sleeves 55 and the magnetic rods 57, so that the magnetic sleeve frames 54 and the magnetic rod frames 56 are mutually overlapped, the magnetic sleeves 55 are sleeved outside the magnetic rods 57 in a one-to-one correspondence manner, the magnetic sleeves 55 and the magnetic rods 57 are integrally transferred to the extraction tube 411 through the third horizontal conveying mechanism 51 to adsorb magnetic beads, and after the magnetic beads are completely adsorbed on the outer surfaces of the magnetic sleeves 55, the magnetic beads, the magnetic sleeves 55 and the magnetic rods 57 are integrally transferred. Finally, when the magnetic beads and the nucleic acids need to be separated, the magnetic rod 57 is lifted by the third lifting mechanism 53, then the magnetic beads and the nucleic acids are separated by stirring the magnetic sleeve 55 and applying a magnetic field, then the magnetic sleeve 55 is lifted to the initial height by the second lifting mechanism 52, and finally the magnetic sleeve 55 and the magnetic rod 57 are integrally conveyed to the initial position by the third horizontal conveying mechanism 51.

Specifically, the third horizontal conveying mechanism 51 and the first horizontal conveying mechanism 31 are respectively installed on the first installation plate 14 at intervals, and the first horizontal conveying mechanism 31, the third horizontal conveying mechanism 51, the first lifting mechanism 33, the second lifting mechanism 52 and the third lifting mechanism 53 are all belt conveying mechanisms. It is understood that the first horizontal conveying mechanism 31, the third horizontal conveying mechanism 51, the first lifting mechanism 33, the second lifting mechanism 52 and the third lifting mechanism 53 may also be other linear conveying mechanisms, such as a ball screw mechanism, which is not limited herein.

In this embodiment, referring to FIG. 2 and FIG. 3, the nucleic acid extraction condition control apparatus 40 is provided with two rows of extraction tube sets 41, the two rows of extraction tube sets 41 are arranged at intervals along the first direction X, and the extraction tube sets 41 in each row of extraction tube sets 41 are arranged at intervals along the second direction Y. Correspondingly, two rows of magnetic sleeves 55 are arranged on the magnetic sleeve frame 54, the two rows of magnetic sleeves 55 are arranged at intervals along the first direction X, and each row of magnetic sleeves 55 are arranged at equal intervals along the second direction Y. Two rows of magnetic rods 57 are arranged on the magnetic rod rack 56, the two rows of magnetic rods 57 are arranged at intervals along the first direction X, and each row of magnetic rods 57 is distributed at equal intervals along the second direction Y. The distance between the two rows of extraction tube groups 41, the distance between the two rows of magnetic sleeves 55, and the distance between the two rows of magnetic rods 57 are equal, and the distance between adjacent extraction tube groups 41, the distance between adjacent magnetic sleeves 55, and the distance between adjacent magnetic rods 57 are equal along the second direction Y. In this embodiment, through the setting of the plurality of groups of extraction tube sets 41, a plurality of nucleic acids can be extracted at one time, and through the setting of the two rows of extraction tube sets 41, the magnetic bead transferring and transferring device can transfer two groups of magnetic beads at the same time, thereby improving the nucleic acid extraction effect of the whole nucleic acid extraction system. It should be understood that, in other embodiments of the present application, the extraction tube set 41, the magnetic sleeve 55 and the magnetic rod 57 may also be three rows or more, and at least two rows may be used, which is not limited herein.

Specifically, in this embodiment, each row of the extracting tube set 41 includes 12 extracting tube sets 41, each row of the magnetic sleeve 55 includes 12 magnetic sleeves 55, each row of the magnetic rods 57 includes 12 magnetic rods 57, so that the magnetic sleeve 55 and the magnetic rod 57 of one magnetic bead transferring and transferring device can simultaneously stir and transfer magnetic beads up and down for reactions in 24 extracting tubes 411, and 24 nucleic acid extracting operations can be performed at a time by the nucleic acid extracting system, thereby greatly improving the nucleic acid extracting efficiency of the nucleic acid extracting system.

Along the second direction Y, at least two groups of extraction tube sets 41 are an integral connection structure, that is, a plurality of extraction tubes 411 are integrally formed, for example, the plurality of integrally connected extraction tubes 411 are formed at one time by injection molding, so that the manufacturing efficiency and the assembling efficiency of the extraction tubes 411 can be improved.

Specifically, in the present embodiment, referring to fig. 3, the four groups of extraction tube sets 41 are an integral connection structure, that is, sixteen extraction tubes 411 are formed together, so that the twenty-four groups of extraction tube sets 41 only need to be formed six times and assembled six times, which greatly improves the manufacturing efficiency and the assembly efficiency of the extraction tubes 411. It is to be understood that in other embodiments of the present application, two, three, five, and more than five groups of extraction tube groups 41 may be integrally formed.

The above description is only exemplary of the present application and should not be taken as limiting the present application, as any modification, equivalent replacement, or improvement made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A nucleic acid extraction system, characterized by: comprises a kit, a reagent conveying device, a nucleic acid extraction condition control device and a magnetic bead transfer device; the kit is characterized in that a plurality of extraction reagents are respectively stored on the kit, the nucleic acid extraction condition control device is used for providing rapid heating control, oscillation mixing control and a plurality of groups of extraction tube groups, each extraction tube group comprises a plurality of extraction tubes which are sequentially arranged along a first direction, a sample reagent is stored in one of the extraction tubes, the reagent conveying device is used for conveying each extraction reagent in the kit to each extraction tube, and the magnetic bead transferring device is used for transferring magnetic beads after reaction of the extraction tubes to the next extraction tube according to a preset sequence; wherein the volume of each extraction tube is 6 ML.

2. The nucleic acid extraction system according to claim 1, wherein: the extraction tube comprises a large-diameter section and a small-diameter section which are mutually connected, the large-diameter section is close to the opening end of the extraction tube, and the small-diameter section is close to the bottom end of the extraction tube; the height of major diameter section is greater than the height of path section, and the internal diameter of major diameter section is greater than the internal diameter of path section.

3. The nucleic acid extraction system according to claim 2, wherein: the cross section of the large-diameter section is square, and the area of the cross section of the large-diameter section is unchanged along the height direction; the cross section of the small-diameter section is circular, and the cross section area of the small-diameter section is gradually reduced from the top end to the bottom end.

4. The nucleic acid extraction system according to any one of claims 1 to 3, wherein: the reagent box is partitioned by a partition board to form a plurality of first reagent grids arranged along the first direction and at least one second reagent grid distributed along the second direction, the first direction is vertical to the second direction, and the extraction reagents in the first reagent grids are correspondingly conveyed to the extraction tubes in each extraction tube group one by one.

5. The nucleic acid extraction system according to any one of claims 1 to 3, wherein: the nucleic acid extraction system further comprises a base frame, the reagent conveying device comprises a first horizontal conveying mechanism arranged on the base frame, a second horizontal conveying mechanism arranged on the first horizontal conveying mechanism, a first lifting mechanism arranged on the second horizontal conveying mechanism, and a suction nozzle frame arranged on the first lifting mechanism, wherein the suction nozzle frame is used for installing suction nozzles and driving the suction nozzles to work; the first horizontal conveying mechanism is used for driving the suction nozzle frame to move along the first direction, and the second horizontal conveying mechanism is used for driving the suction nozzle frame to move along the second direction.

6. The nucleic acid extraction system according to claim 5, wherein: the nucleic acid extraction system further comprises a suction nozzle supply area and a suction nozzle removal area, wherein the suction nozzle supply area and the suction nozzle removal area are respectively arranged on the base frame, the suction nozzle supply area is used for supplying suction nozzles to the suction nozzle frame, and the suction nozzle removal area is used for removing the used suction nozzles on the suction nozzle frame.

7. The nucleic acid extraction system according to claim 6, wherein: the suction nozzle is characterized in that a suction nozzle removing frame is arranged on the suction nozzle removing area, an open slot is formed in the suction nozzle removing frame and used for clamping the suction nozzle so that the suction nozzle can be taken off from the suction nozzle frame, and after the suction nozzle is clamped, the open slot is in interference fit with the suction nozzle.

8. The nucleic acid extraction system according to claim 5, wherein: the magnetic bead transfer device comprises a third horizontal conveying mechanism arranged on the base frame, a second lifting mechanism and a third lifting mechanism which are arranged on the third horizontal conveying mechanism through the same mounting seat, a magnetic sleeve frame which is arranged on the second lifting mechanism and can be driven by the second lifting mechanism to do lifting motion, a plurality of magnetic sleeves arranged on the magnetic sleeve frame, a magnetic bar frame which is arranged on the third lifting mechanism and can be driven by the third lifting mechanism to do lifting motion, and a plurality of magnetic bars arranged on the magnetic bar frame, wherein each magnetic sleeve and each magnetic bar are arranged in a one-to-one correspondence mode along the vertical direction.

9. The nucleic acid extraction system of claim 8, wherein: the nucleic acid extraction condition control means is provided with at least two rows of extraction tube groups, each row of the extraction tube groups being arranged at intervals in the first direction, and each of the extraction tube groups in each row being arranged at intervals in the second direction; the magnetic sleeve rack is provided with at least two rows of magnetic sleeves, and the magnetic rod rack is provided with at least two rows of magnetic rods.

10. The nucleic acid extraction system of claim 9, wherein: along the second direction, at least two groups of extraction tube groups are of an integral connecting structure.