CN105296330B - Liquid level inclined reagent cartridge when a kind of material transfer - Google Patents
Liquid level inclined reagent cartridge when a kind of material transfer Download PDFInfo
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- CN105296330B CN105296330B CN201510785325.XA CN201510785325A CN105296330B CN 105296330 B CN105296330 B CN 105296330B CN 201510785325 A CN201510785325 A CN 201510785325A CN 105296330 B CN105296330 B CN 105296330B
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- magnetic bead
- chamber
- shifts
- liquid level
- extraction platform
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
Abstract
When a kind of transfer of material the inclined reagent cartridge of liquid level include bottom plate, one end and the bottom plate pin joint extraction platform, the extraction platform is equipped with first chamber and second chamber and by the first chamber and second chamber accommodating chamber spaced apart, and the magnetic bead transfer organization being transferred to the magnetic bead in first chamber in second chamber is provided in the accommodating chamber；When the material shifts the inclined reagent cartridge of liquid level further include extraction platform the other end install by the inclined leaning device of the extraction platform.The inclined reagent cartridge of liquid level installs leaning device in extraction platform when material transfer provided in an embodiment of the present invention, tilt operation is carried out to extraction platform when magnetic bead during nucleic acid extraction shifts, magnetic bead is set to be detached before magnetic bead transfer with liquid, the liquid incidental impurities generated together with magnetic bead transfer process are avoided, the DNA purity and efficiency of material to be extracted (protein, nucleic acid) are improved.
Description
Technical field
Liquid level inclined reagent card when being shifted the present invention relates to the technical field of nucleic acid extraction more particularly to a kind of material
Box.
Background technology
With the transformation of medical model and the continuous development of personalized medicine, there is an urgent need to quick, accurate for medical test circle
Detection means, Molecular Detection then plays unique advantage.The basis of Molecular Detection be analyze detected person histocyte,
Hair, anticoagulation or dry bloodstain and formaldehyde fixes, gene and its expression product in the tissue of paraffin embedding, by from point
Nucleic acid (DNA and RNA) is completed in sub- level to detect, in disease once occurring even symptom, sign and biochemical change not yet occur
Before, detection can accurately just be made.
Currently, molecular detection technology mainly has making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology
Deng.Molecular Detection product is mainly used in the detection and physical examination of the clinical departments such as tumour, infection, heredity, Prenatal Screening
The heart, technical service center, third party testing agency and microbial rapid detection market etc..Currently, blood routine, cell
The measuring means such as, pathology and immunology develop towards automation, integration, standardization direction, but due to Molecular Detection
The full-automatic instrument platform of its own technical complexity, " from sample to result " is few or there are many insoluble skills
Art problem.For example, the centrifugal column method or paramagnetic particle method that are used in biotechnology carry out nucleic acid extraction, generally requires and split
Four steps such as solution, combination, rinsing, elution, in addition subsequent making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biological core
The detecting steps such as piece keep the full-automatic instrument of entire " from sample to result " very difficult, single with regard to effective in each step
For the transfer of ingredient, the prior art mainly adopts the modes shifted manually, i.e., by artificial mode by raw material or centre
Product is shifted in the reaction vessel of step successively in step successively and realizing, it is therefore apparent that the hand used in the prior art
Animal material branch mode, it is not only cumbersome, time-consuming and laborious.Wherein, during existing nucleic acid extraction, most of nucleic acid carries
The indoor liquid level of each chamber of card taking box or nucleic acid extraction structure is usually constantly in together with entire extraction platform horizontal
State, and in magnetic bead in the indoor transfer process of adjacent chambers, magnetic bead is dipped in extracts reagent or the reaction solution of intracavitary, magnetic bead
The extracts reagent or reaction solution that transfer can take away the extracts reagent of intracavitary either reaction solution and intracavitary together dissolve residual
Impurity, influence the purity and efficiency of extract, and then have an adverse effect to subsequent experimental result.
Invention content
For deficiency in the prior art, present invention solves the technical problem that being to provide a kind of simple in structure, operation side
Just liquid level inclined reagent cartridge when material shifts, magnetic bead during nucleic acid extraction incline to extraction platform when shifting
Tiltedly operation makes magnetic bead be detached before magnetic bead transfer with liquid, avoids the liquid generated together with magnetic bead transfer process attached
Band impurity, improves the DNA purity and efficiency of material to be extracted (protein, nucleic acid).
In order to solve the above-mentioned technical problem, liquid level inclined reagent cartridge packet when material transfer provided in an embodiment of the present invention
Include bottom plate, one end and the bottom plate pin joint extraction platform, the extraction platform be equipped with first chamber and second chamber and
By the first chamber and second chamber accommodating chamber spaced apart, it is provided in the accommodating chamber and turns the magnetic bead in first chamber
Move to the magnetic bead transfer organization in second chamber；The inclined reagent cartridge of liquid level further includes in extraction platform when the material shifts
Other end installation by the inclined leaning device of the extraction platform.
As the preferred embodiment of the present invention, liquid level inclined reagent when a kind of material that the embodiment of the present invention provides shifts
Cartridge further comprises some or all of following technical characteristic：
In one embodiment of this invention, the leaning device includes being located at the plate upper surface and extraction platform following table
Flexible thimble between face, the flexible thimble move up and down under the driving of external force and the extraction platform are driven to be in water
Flat or heeling condition.
In another embodiment of the invention, the leaning device include top plate, be located at the top plate and extraction platform it
Between pulley assembly, the drawstring that is serially connected in the pulley assembly, a free end of the drawstring is fixedly connected on described carry
It makes even the other end of platform, another free end of the drawstring is connected with traction motor.
In another embodiment of the invention, the leaning device is spring assist lifting gear, including is located at the bottom
The spring of elevating lever, the connection elevating lever and pedestal that pedestal, one end on plate are connect with the base rotation, the lifting
The other end of bar is connected to the extraction platform.
Preferably, the magnetic bead transfer organization includes cylindricality rotational structure and pushes the silk of the cylindricality rotational structure rotation
Bar, the cylindricality rotational structure are provided with a threaded hole along its central axes, internal thread are provided in the threaded hole, the internal thread
Match with the external screw thread of lead screw outer surface.
Preferably, the side of the cylindricality rotational structure is provided with the adsorbing plane for accommodating magnetic bead, is gone back in the accommodating chamber
It is provided with the magnet of absorption magnetic bead；The adsorbing plane shifts the previous dynasty to the first chamber in magnetic bead, and the magnet is by magnetic bead
It is adsorbed in the adsorbing plane；When magnetic bead shifts, lead screw, lead screw is axially pushed to enter cylindricality rotation knot along cylindricality rotational structure
In the threaded hole of structure, the internal thread that the external screw thread of lead screw acts on threaded hole pushes cylindricality rotational structure around its axis in accommodating chamber
The magnetic bead of interior rotation, the adsorbing plane and adsorbing plane attachment is turned to towards in the region of second chamber.
Preferably, the lead screw is provided with a through-hole along wherein axis direction, the magnet intert when magnetic bead shifts into
Magnetic bead is adsorbed in the through-hole, the magnet exits the through-hole after completing magnetic bead transfer.
Liquid level inclined reagent cartridge when material transfer provided in an embodiment of the present invention, has the advantages that：
The inclined reagent cartridge of liquid level installs dumper in extraction platform when material transfer provided in an embodiment of the present invention
Structure carries out tilt operation when magnetic bead during nucleic acid extraction shifts to extraction platform, and magnetic bead is made to be shifted in magnetic bead with liquid
It is preceding i.e. detach, avoid the liquid incidental impurities generated together with magnetic bead transfer process, improve material to be extracted (protein,
Nucleic acid) DNA purity and efficiency.Accommodating chamber is arranged in the present invention between first chamber and second chamber, is arranged in accommodating chamber
Magnetic bead transfer organization, the side of the cylindricality rotational structure in the magnetic bead transfer organization are provided with the adsorbing plane for accommodating magnetic bead, inhale
Attached plane shifts the previous dynasty to the first chamber in magnetic bead, and magnetic bead is adsorbed in the adsorbing plane by magnet, therefore, the present invention
During nucleic acid extraction magnetic bead transfer when extraction platform is obliquely installed, make the indoor liquid level of each chamber be not with it is whole
A extraction platform be constantly in together horizontality extraction nucleic acid it is abundant, in magnetic bead in the indoor transfer process of adjacent chambers, make
Magnetic bead is detached with liquid before magnetic bead transfer, improves the DNA purity of nucleic acid.
Description of the drawings
Fig. 1 is the structural schematic diagram of liquid level inclined reagent cartridge when the material that the embodiment of the present invention one provides shifts.
The structural schematic diagram of the inclined reagent cartridge of liquid level when Fig. 2 is material transfer provided by Embodiment 2 of the present invention.
Fig. 3 is the structural schematic diagram of liquid level inclined reagent cartridge when the material that the embodiment of the present invention three provides shifts.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other without creative efforts
Embodiment shall fall within the protection scope of the present invention.
The inclined reagent cartridge of liquid level is applied in nucleic acid preparation and detection device when the material transfer of the present invention, it is intended to be
Nucleic acid prepare and detection process in material transfer between adjacent step provide it is a kind of efficiently, efficiently solution, but not
As limit, in practical applications, the extraction of other biological extract can also be applied to.
Embodiment one
Such as Fig. 1, the inclined reagent cartridge of liquid level includes bottom plate 10, one when the material that the embodiment of the present invention one provides shifts
End and the extraction platform 30 of 10 pin joint of the bottom plate, the extraction platform 30 are equipped with first chamber and second chamber and by institutes
The accommodating chamber that first chamber and second chamber are spaced apart is stated, is provided in the accommodating chamber and is transferred to the magnetic bead in first chamber
Magnetic bead transfer organization in second chamber；The inclined reagent cartridge of liquid level further includes in the another of extraction platform when the material shifts
End installation by the inclined leaning device 40 of the extraction platform, the leaning device 40 include be located at the plate upper surface and
Flexible thimble 41 between extraction platform lower surface, the flexible thimble 41 move up and down under the driving of external force and drive institute
It states extraction platform 30 and is in horizontally or diagonally state, it is right by flexible thimble 41 when magnetic bead during nucleic acid extraction shifts
Extraction platform 30 carries out tilt operation, so that magnetic bead is detached before magnetic bead transfer with liquid, avoids with magnetic bead transfer process
The liquid incidental impurities generated together improve the DNA purity and efficiency of material to be extracted (protein, nucleic acid).
When specific implementation, magnetic bead transfer organization includes cylindricality rotational structure and pushes the lead screw of cylindricality rotational structure rotation,
During specific implementation, cylindricality rotational structure be set in accommodating chamber and with accommodating chamber formed sealing accommodating chamber by first chamber and
Second chamber separates, and cylindricality rotational structure is provided with a threaded hole along its central axes, and internal thread is provided in threaded hole, the interior spiral shell
Line and the external screw thread of lead screw outer surface match.When lead screw is longitudinally propelling along cylindricality rotational structure, pass through screw-driven, silk
Bar axial translation is converted into the rotation of cylindricality rotational structure, and cylindricality rotational structure rotates in accommodating chamber.
In order to fully, efficiently shift magnetic bead, the side of cylindricality rotational structure be provided with accommodate magnetic bead absorption it is flat
Face is additionally provided with the magnet (not shown) of absorption magnetic bead in accommodating chamber.It is, in principle, that the present invention is mainly by using
Magnetic-particle including magnetic bead is as extraction nucleic acid or other macromolecular bio-extracts, and magnetic bead is in first chamber realization pair
The suction-operated of nucleic acids in samples or other macromolecular bio-extracts is adsorbed on the magnetic bead in first chamber in mixed material
Nucleic acid to be extracted or other macromolecular bio-extracts.At this point, i.e. before magnetic bead transfer, adsorbing plane is towards first chamber, magnetic
Magnetic bead is adsorbed in adsorbing plane by body；When magnetic bead shifts, lead screw, lead screw is axially pushed to enter cylindricality along cylindricality rotational structure
In the threaded hole of rotational structure, the internal thread that the external screw thread of lead screw acts on threaded hole pushes cylindricality rotational structure to exist around its axis
Rotation in accommodating chamber, adsorbing plane and the interior magnetic bead accommodated of adsorbing plane are turned to towards in the region of second chamber.Passing through will
The side local setting of cylindricality rotational structure is adsorbing plane, and adsorbing plane is made, to first chamber, to wait for magnetic in the absorption previous dynasty
Pearl is adsorbed onto under the magnetic force on the adsorbing plane, then pushes cylindricality rotational structure to rotate through accommodating chamber by lead screw,
Arcuate gap can be formed between adsorbing plane and the curved surface cavity wall of accommodating chamber in rotation process, and is adsorbed on adsorbing plane
On magnetic bead fallen off without being received the cavity wall of chamber and scraping off because of the presence of arcuate gap thus, to realize, effectively material turns
Fortune, while being matched in cylindricality rotational structure except other positions of adsorbing plane can be sealed with accommodating chamber, it ensure that in addition to magnetic bead
Mutual independence of the unclassified stores between first chamber and second chamber.
In addition, from the point of view of the structure setting of the joining place of first chamber and second chamber and accommodating chamber, in order to fully,
Efficiently shift magnetic bead, the joining place of first chamber and accommodating chamber is set as the contraction structure become narrow gradually, first chamber with
The cohesive position of cylindricality rotational structure, first chamber are contracted to width corresponding with the adsorbing plane.Correspondingly, second
Chamber and the joining place of accommodating chamber are also configured as the contraction structure become narrow gradually, in being connected for second chamber and cylindricality rotational structure
Position, second chamber are contracted to width corresponding with the adsorbing plane.For this purpose, first chamber and second chamber and accommodating chamber
Joining place may be configured as the structure that chamfering receives side.
In a preferred embodiment of the invention, first chamber and second chamber are arranged in parallel, therefore, when magnetic bead shifts, silk
Bar needs that cylindricality rotational structure is pushed to rotate half cycle in accommodating chamber, and correspondingly, the axial translation of lead screw promotes this
Size is half pitch.Certainly in other embodiments of the present invention, first chamber not parallel can be arranged with second chamber,
And there are certain angles for the two, and at this point, lead screw promotes size and cylindricality rotational structure angle also by first chamber and the
Angle between two chambers is determined, to enable the magnetic bead in first chamber to be accurately and efficiently transferred to second chamber
In.
In the embodiment of the present invention one, lead screw is provided with a through-hole along wherein axis direction, and magnet is when magnetic bead shifts
Intert and adsorb magnetic bead in through-hole, magnet exits through-hole after completing magnetic bead transfer.In this scheme, the work side of magnetic bead transfer organization
Formula is：Before magnetic bead transfer, for adsorbing plane towards first chamber, magnet, which interts, adsorbs magnetic bead in through-hole, magnetic bead is adsorbed to
In adsorbing plane；When magnetic bead shifts, lead screw, lead screw is axially pushed to enter the screw thread of cylindricality rotational structure along cylindricality rotational structure
Kong Zhong, the internal thread that the external screw thread of lead screw acts on threaded hole push cylindricality rotational structure to be rotated in accommodating chamber around its axis,
The magnetic bead accommodated in adsorbing plane and adsorbing plane is turned to towards in the region of second chamber；When adsorbing plane and adsorbing plane
The magnetic bead of interior receiving is turned to towards after second chamber, and magnet exits in through-hole, and magnetic bead is made due to losing the absorption of magnet magnetic force
With being dispersed in second chamber.
In an embodiment of the present invention, there are two be parallel to cylindricality rotational structure axis for the both sides setting of magnetic bead transfer organization
Magnet, magnet generates the opposite toroidal magnetic field in direction, to make magnetic bead be moved to adsorbing plane.In this scheme, magnetic bead transfer
The working method of structure is：Before magnetic bead transfer, adsorbing plane towards first chamber, make by the magnetic force that magnetic bead is formed in toroidal magnetic field
It under, concentrates in adsorbing plane, and this magnetic direction is identical as the shift direction of magnetic bead；When magnetic bead shifts, along cylindricality
Rotational structure axially pushes lead screw, lead screw to enter in the threaded hole of cylindricality rotational structure, and the external screw thread of lead screw acts on threaded hole
Internal thread push cylindricality rotational structure to be rotated in accommodating chamber around its axis, the magnetic bead accommodated in adsorbing plane and adsorbing plane
It turns to towards in the region of second chamber.
Embodiment two
Such as Fig. 2, the inclined reagent cartridge of liquid level includes bottom plate 10, one when material provided by Embodiment 2 of the present invention shifts
End and the extraction platform 30 of 10 pin joint of the bottom plate, the extraction platform 30 are equipped with first chamber and second chamber and by institutes
The accommodating chamber that first chamber and second chamber are spaced apart is stated, is provided in the accommodating chamber and is transferred to the magnetic bead in first chamber
Magnetic bead transfer organization in second chamber；The inclined reagent cartridge of liquid level further includes in the another of extraction platform when the material shifts
End installation by the inclined leaning device 40 of the extraction platform, the leaning device 40 includes top plate 20, is located at the top plate
Pulley assembly 43 between 20 and extraction platform 30, the drawstring 44 being serially connected in the pulley assembly 43, the one of the drawstring 44
A free end is fixedly connected on the other end of the extraction platform 30, another free end of the drawstring 44 is connected with traction horse
Up to 45, traction motor 45 generates drawstring 44 downward pulling force, to make extraction platform 30 tilt, and then makes first chamber, the
Two chambers and transfer chamber tilt, when magnetic bead transfer during nucleic acid extraction by pulley assembly 43 to extraction platform 30 into
Line tilt operates, and so that magnetic bead is detached before magnetic bead transfer with liquid, avoids the liquid generated together with magnetic bead transfer process
Body incidental impurities improve the DNA purity and efficiency of material to be extracted (protein, nucleic acid).
When specific implementation, magnetic bead transfer organization includes cylindricality rotational structure and pushes the lead screw of cylindricality rotational structure rotation,
During specific implementation, cylindricality rotational structure be set in accommodating chamber and with accommodating chamber formed sealing accommodating chamber by first chamber and
Second chamber separates, and cylindricality rotational structure is provided with a threaded hole along its central axes, and internal thread is provided in threaded hole, the interior spiral shell
Line and the external screw thread of lead screw outer surface match.When lead screw is longitudinally propelling along cylindricality rotational structure, pass through screw-driven, silk
Bar axial translation is converted into the rotation of cylindricality rotational structure, and cylindricality rotational structure rotates in accommodating chamber.
In order to fully, efficiently shift magnetic bead, the side of cylindricality rotational structure be provided with accommodate magnetic bead absorption it is flat
Face is additionally provided with the magnet (not shown) of absorption magnetic bead in accommodating chamber.It is, in principle, that the present invention is mainly by using
Magnetic-particle including magnetic bead is as extraction nucleic acid or other macromolecular bio-extracts, and magnetic bead is in first chamber realization pair
The suction-operated of nucleic acids in samples or other macromolecular bio-extracts is adsorbed on the magnetic bead in first chamber in mixed material
Nucleic acid to be extracted or other macromolecular bio-extracts.At this point, i.e. before magnetic bead transfer, adsorbing plane is towards first chamber, magnetic
Magnetic bead is adsorbed in adsorbing plane by body；When magnetic bead shifts, lead screw, lead screw is axially pushed to enter cylindricality along cylindricality rotational structure
In the threaded hole of rotational structure, the internal thread that the external screw thread of lead screw acts on threaded hole pushes cylindricality rotational structure to exist around its axis
Rotation in accommodating chamber, adsorbing plane and the interior magnetic bead accommodated of adsorbing plane are turned to towards in the region of second chamber.Passing through will
The side local setting of cylindricality rotational structure is adsorbing plane, and adsorbing plane is made, to first chamber, to wait for magnetic in the absorption previous dynasty
Pearl is adsorbed onto under the magnetic force on the adsorbing plane, then pushes cylindricality rotational structure to rotate through accommodating chamber by lead screw,
Arcuate gap can be formed between adsorbing plane and the curved surface cavity wall of accommodating chamber in rotation process, and is adsorbed on adsorbing plane
On magnetic bead fallen off without being received the cavity wall of chamber and scraping off because of the presence of arcuate gap thus, to realize, effectively material turns
Fortune, while being matched in cylindricality rotational structure except other positions of adsorbing plane can be sealed with accommodating chamber, it ensure that in addition to magnetic bead
Mutual independence of the unclassified stores between first chamber and second chamber.
In addition, from the point of view of the structure setting of the joining place of first chamber and second chamber and accommodating chamber, in order to fully,
Efficiently shift magnetic bead, the joining place of first chamber and accommodating chamber is set as the contraction structure become narrow gradually, first chamber with
The cohesive position of cylindricality rotational structure, first chamber are contracted to width corresponding with the adsorbing plane.Correspondingly, second
Chamber and the joining place of accommodating chamber are also configured as the contraction structure become narrow gradually, in being connected for second chamber and cylindricality rotational structure
Position, second chamber are contracted to width corresponding with the adsorbing plane, for this purpose, first chamber and second chamber and accommodating chamber
Joining place may be configured as the structure that chamfering receives side.
In a preferred embodiment of the invention, first chamber and second chamber are arranged in parallel, therefore, when magnetic bead shifts, silk
Bar needs that cylindricality rotational structure is pushed to rotate half cycle in accommodating chamber, and correspondingly, the axial translation of lead screw promotes this
Size is half pitch.Certainly in other embodiments of the present invention, first chamber not parallel can be arranged with second chamber,
And there are certain angles for the two, and at this point, lead screw promotes size and cylindricality rotational structure angle also by first chamber and the
Angle between two chambers is determined, to enable the magnetic bead in first chamber to be accurately and efficiently transferred to second chamber
In.
In the embodiment of the present invention two, lead screw is provided with a through-hole along wherein axis direction, and magnet is when magnetic bead shifts
Intert and adsorb magnetic bead in through-hole, magnet exits through-hole after completing magnetic bead transfer.In this scheme, the work side of magnetic bead transfer organization
Formula is：Before magnetic bead transfer, for adsorbing plane towards first chamber, magnet, which interts, adsorbs magnetic bead in through-hole, magnetic bead is adsorbed to
In adsorbing plane；When magnetic bead shifts, lead screw, lead screw is axially pushed to enter the screw thread of cylindricality rotational structure along cylindricality rotational structure
Kong Zhong, the internal thread that the external screw thread of lead screw acts on threaded hole push cylindricality rotational structure to be rotated in accommodating chamber around its axis,
The magnetic bead accommodated in adsorbing plane and adsorbing plane is turned to towards in the region of second chamber；When adsorbing plane and adsorbing plane
The magnetic bead of interior receiving is turned to towards after second chamber, and magnet exits in through-hole, and magnetic bead is made due to losing the absorption of magnet magnetic force
With being dispersed in second chamber.
In an embodiment of the present invention, there are two be parallel to cylindricality rotational structure axis for the both sides setting of magnetic bead transfer organization
Magnet, magnet generates the opposite toroidal magnetic field in direction, to make magnetic bead be moved to adsorbing plane.In this scheme, magnetic bead transfer
The working method of structure is：Before magnetic bead transfer, adsorbing plane towards first chamber, make by the magnetic force that magnetic bead is formed in toroidal magnetic field
It under, concentrates in adsorbing plane, and this magnetic direction is identical as the shift direction of magnetic bead；When magnetic bead shifts, along cylindricality
Rotational structure axially pushes lead screw, lead screw to enter in the threaded hole of cylindricality rotational structure, and the external screw thread of lead screw acts on threaded hole
Internal thread push cylindricality rotational structure to be rotated in accommodating chamber around its axis, the magnetic bead accommodated in adsorbing plane and adsorbing plane
It turns to towards in the region of second chamber.
Embodiment three
As shown in figure 3, the inclined reagent cartridge of liquid level is carried with implementing one when the material that the embodiment of the present invention three provides shifts
The inclined reagent cartridge of liquid level differs only in following aspect when the material transfer of confession：The leaning device 40 is spring assist
Lifting gear, include pedestal 42 on the bottom plate, one end be rotatablely connected with the pedestal 42 elevating lever 46, connect
The other end of the spring 47 of the elevating lever 46 and pedestal 42, the elevating lever 46 is connected to the extraction platform 30, in nucleic acid
Tilt operation is carried out to extraction platform 30 by spring assist lifting gear when magnetic bead in extraction process shifts, makes magnetic bead and liquid
Body is detached before magnetic bead transfer, avoids the liquid incidental impurities generated together with magnetic bead transfer process, is improved to be extracted
The DNA purity and efficiency of material (protein, nucleic acid).
For magnet selection with from the point of view of material, when specific implementation, magnet is permanent magnet or electromagnet.The present invention's
Permanent magnet or can be extraordinary preferred embodiment by the electromagnet of power control is selected in embodiment.And in the present invention
In embodiment one to embodiment three, required toroidal magnetic field, and magnetic can not only easily and efficiently be generated using electromagnet
Action time (i.e. magnetic bead transfer time) can be accurately controlled, magnetic bead is from suction after magnetic bead is transferred to second chamber
It is further reacted in rapid dispersion to second chamber in attached plane.
The inclined reagent cartridge of liquid level installs dumper in extraction platform 30 when the material transfer that above-described embodiment discloses
Structure 40 carries out tilt operation when magnetic bead during nucleic acid extraction shifts to extraction platform 30, makes magnetic bead with liquid in magnetic bead
It is detached before transfer, avoids the liquid incidental impurities generated together with magnetic bead transfer process, improve material (albumen to be extracted
Matter, nucleic acid) DNA purity and efficiency.Accommodating chamber is arranged in the present invention between first chamber and second chamber, in accommodating chamber
Magnetic bead transfer organization is set, the side of the cylindricality rotational structure in the magnetic bead transfer organization be provided with accommodate magnetic bead absorption it is flat
Face, adsorbing plane shift the previous dynasty to the first chamber in magnetic bead, and magnetic bead is adsorbed in the adsorbing plane by magnet, therefore,
The present invention is obliquely installed extraction platform 30 when magnetic bead during nucleic acid extraction shifts, and makes the indoor liquid level of each chamber
Be not with entire extraction platform 30 be constantly in together horizontality extraction nucleic acid it is abundant, in magnetic bead in the indoor transfer of adjacent chambers
In the process, so that magnetic bead is detached before magnetic bead transfer with liquid, improve the DNA purity of nucleic acid.
The above is the preferred embodiment of the present invention, it is noted that for those skilled in the art
For, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also considered as
Protection scope of the present invention.
Claims (6)
1. liquid level inclined reagent cartridge when a kind of material transfer, which is characterized in that including bottom plate, one end and the bottom plate pin joint
Extraction platform, the extraction platform is equipped with first chamber and second chamber and will be between the first chamber and second chamber
The accommodating chamber separated is provided with the magnetic bead that the magnetic bead in first chamber is transferred in second chamber in the accommodating chamber and shifts knot
Structure；When the material shifts the inclined reagent cartridge of liquid level further include extraction platform the other end install by the extraction platform
Inclined leaning device;The magnetic bead transfer organization includes cylindricality rotational structure and the cylindricality rotational structure is pushed to rotate
Lead screw, the cylindricality rotational structure are provided with a threaded hole along its central axes, internal thread are provided in the threaded hole, the interior spiral shell
Line and the external screw thread of lead screw outer surface match.
2. liquid level inclined reagent cartridge when material transfer as described in claim 1, it is characterised in that：The cylindricality rotation knot
The side of structure is provided with the adsorbing plane for accommodating magnetic bead, and the magnet of absorption magnetic bead is additionally provided in the accommodating chamber；The absorption
Plane shifts the previous dynasty to the first chamber in magnetic bead, and magnetic bead is adsorbed in the adsorbing plane by the magnet；Magnetic bead shifts
When, axially push lead screw, lead screw to enter in the threaded hole of cylindricality rotational structure along cylindricality rotational structure, the external screw thread effect of lead screw
Cylindricality rotational structure is pushed to be rotated in accommodating chamber around its axis in the internal thread of threaded hole, the adsorbing plane and adsorbing plane
The magnetic bead of attachment is turned to towards in the region of second chamber.
3. liquid level inclined reagent cartridge when material transfer as claimed in claim 2, it is characterised in that：The lead screw is along wherein
Axis direction is provided with a through-hole, and the magnet interts when magnetic bead shifts adsorbs magnetic bead in the through-hole, completes magnetic bead and turns
The magnet exits the through-hole after shifting.
Liquid level inclined reagent cartridge when 4. the material as described in any one of claim 1-3 shifts, it is characterised in that：Institute
It includes the flexible thimble between the plate upper surface and extraction platform lower surface to state leaning device, and the flexible thimble exists
It moves up and down under the driving of external force and the extraction platform is driven to be in horizontally or diagonally state.
Liquid level inclined reagent cartridge when 5. the material as described in any one of claim 1-3 shifts, it is characterised in that：Institute
Leaning device is stated to include top plate, the pulley assembly between the top plate and extraction platform, be serially connected in the pulley assembly
Drawstring, a free end of the drawstring is fixedly connected on the other end of the extraction platform, the drawstring another from
Traction motor is connected with by end.
Liquid level inclined reagent cartridge when 6. the material as described in any one of claim 1-3 shifts, it is characterised in that：Institute
It is spring assist lifting gear to state leaning device, includes that pedestal on the bottom plate, one end are connect with the base rotation
Elevating lever, the connection elevating lever and pedestal spring, the other end of the elevating lever is connected to the extraction platform.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510785325.XA CN105296330B (en) | 2015-11-13 | 2015-11-13 | Liquid level inclined reagent cartridge when a kind of material transfer |
| PCT/CN2016/113553 WO2017080531A1 (en) | 2015-11-13 | 2016-12-30 | Reagent cartridge which tilts liquid surface during material transfer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| CN105296330B true CN105296330B (en) | 2018-10-02 |
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| CN105296330B (en) * | 2015-11-13 | 2018-10-02 | 奥然生物科技（上海）有限公司 | Liquid level inclined reagent cartridge when a kind of material transfer |
| CN113496930A (en) * | 2020-04-03 | 2021-10-12 | 重庆超硅半导体有限公司 | Silicon wafer nondestructive transfer method for integrated circuit |
| CN113049495A (en) * | 2021-03-24 | 2021-06-29 | 东莞东阳光医疗智能器件研发有限公司 | Chemiluminescence detection equipment |
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| CN101613039A (en) * | 2009-06-19 | 2009-12-30 | 东南大学 | The biomass feed divider of a kind of changeable trough obliquity and oscillation frequency and amplitude |
| CN102665913A (en) * | 2009-07-31 | 2012-09-12 | 西蒙·斯塔福德 | Means for improved liquid handling in a microplate |
| CN104425329A (en) * | 2013-08-30 | 2015-03-18 | 亚智科技股份有限公司 | Substrate transfer and processing system and substrate transfer and processing method |
| CN204607980U (en) * | 2015-04-10 | 2015-09-02 | 余家昌 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
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| US5380493A (en) * | 1992-03-27 | 1995-01-10 | Chavez; Jeffery B. | Cell well plate holder and well marking system |
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| CN106148184B (en) * | 2015-04-09 | 2018-08-31 | 奥然生物科技（上海）有限公司 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
| CN105087379A (en) * | 2015-09-10 | 2015-11-25 | 佛山市百特思舞台设备有限公司 | Magnetic substance extraction method and special magnetic frame for magnetic substance extraction |
| CN204999920U (en) * | 2015-09-10 | 2016-01-27 | 佛山市百特思舞台设备有限公司 | Special magnetic shelf that magnetic substance drawed |
| CN105296330B (en) * | 2015-11-13 | 2018-10-02 | 奥然生物科技（上海）有限公司 | Liquid level inclined reagent cartridge when a kind of material transfer |
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| CN101613039A (en) * | 2009-06-19 | 2009-12-30 | 东南大学 | The biomass feed divider of a kind of changeable trough obliquity and oscillation frequency and amplitude |
| CN102665913A (en) * | 2009-07-31 | 2012-09-12 | 西蒙·斯塔福德 | Means for improved liquid handling in a microplate |
| CN104425329A (en) * | 2013-08-30 | 2015-03-18 | 亚智科技股份有限公司 | Substrate transfer and processing system and substrate transfer and processing method |
| CN204608019U (en) * | 2015-04-09 | 2015-09-02 | 余家昌 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
| CN204607980U (en) * | 2015-04-10 | 2015-09-02 | 余家昌 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
| CN205188260U (en) * | 2015-11-13 | 2016-04-27 | 余家昌 | Reagent card box of liquid level slope when material shifts |
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| CN105296330A (en) | 2016-02-03 |
| WO2017080531A1 (en) | 2017-05-18 |
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