CN105950438B - A kind of reagent cartridge being provided with push type magnetic bead transfer device - Google Patents
A kind of reagent cartridge being provided with push type magnetic bead transfer device Download PDFInfo
- Publication number
- CN105950438B CN105950438B CN201610329938.7A CN201610329938A CN105950438B CN 105950438 B CN105950438 B CN 105950438B CN 201610329938 A CN201610329938 A CN 201610329938A CN 105950438 B CN105950438 B CN 105950438B
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- rotor
- magnetic bead
- cavity
- transfer device
- push rod
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Abstract
A kind of reagent cartridge being provided with push type magnetic bead transfer device includes the first cavity being disposed adjacent and the second cavity and the interval region that first cavity and the second cavity is spaced apart；An accommodating chamber is formed in the interval region, it is provided with one in the accommodating chamber, the intracorporal magnetic bead of the first chamber is transferred to the intracorporal push type magnetic bead transfer device of the second chamber, the push type magnetic bead transfer device sealing is embedded in the accommodating chamber, the push type magnetic bead transfer device includes rotor and the push rod for pushing the rotor rotation, and the rotor bottom is provided with the cylindricality rotational structure driven by rotor.The solution of the present invention makes magnetic bead and is adsorbed in the material to be extracted of magnetic bead sufficiently, efficiently shifted by the way that push type magnetic bead transfer device is arranged between adjacent cavity；In addition, not only self structure is simple for reagent cartridge of the invention, but also easy to operate, the complexity of the matched equipment used can be effectively reduced.
Description
Technical field
The present invention relates to the preparation of nucleic acid or the protein consumptive materials more particularly to one kind used with detection to be provided with push type
The reagent cartridge of magnetic bead transfer device.
Background technique
With the transformation of medical model and the continuous development of personalized medicine, there is an urgent need to quick, accurate for medical test circle
Detection means, Molecular Detection then plays unique advantage.The basis of Molecular Detection be analyze detected person histocyte,
Hair, anticoagulation or dry bloodstain and formaldehyde fixes, gene and its expression product in the tissue of paraffin embedding, by from point
Nucleic acid (DNA and RNA) detection is completed in sub- level, in disease once occurring symptom, sign and biochemical change even not yet occur
Before, detection can accurately just be made.
Currently, molecular detection technology mainly has making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology
Deng.Molecular Detection product is mainly used in the detection and physical examination of the clinical departments such as tumour, infection, heredity, Prenatal Screening
The heart, technical service center, third party testing agency and microbial rapid detection market etc..Currently, blood routine, cell
The measuring means such as, pathology and immunology develop towards automation, integration, standardization direction, but due to Molecular Detection
The full-automatic instrument platform of its own technical complexity, " from sample to result " is few or there are many insoluble skills
Art problem.For example, the centrifugal column method used in field of biotechnology or paramagnetic particle method carry out nucleic acid extraction, generally requires and split
Four steps such as solution, combination, rinsing, elution, in addition subsequent making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biological core
The detecting steps such as piece keep the full-automatic instrument of entire " from sample to result " very difficult, single with regard to effective in each step
For the transfer of ingredient, the prior art mainly adopts the modes shifted manually, i.e., by artificial mode by raw material or centre
Product is successively shifted in the reaction vessel of step in successively step and realizing, it is therefore apparent that the hand used in the prior art
Animal material branch mode, it is not only cumbersome, time-consuming and laborious.What is more also resides in whole operation process and be easy to cause pollution,
The purity of extract is influenced, and material is difficult sufficiently, efficiently to be shifted, and influences experimental result.Therefore, in view of Molecular Detection
The full-automatic instrument platform of its own technical complexity, " from sample to result " is few or there are many insoluble skills
Art problem, to realize material between each step it is abundant, efficiently shift the structure and function just needed in consumptive material and experimental facilities
Can go up carry out it is various consider, can avoid that such as at high cost, poor sensitivity existing in the prior art, structure is complicated, behaviour
Make the problems such as excessively complicated with associated assay devices.
Summary of the invention
Aiming at the shortcomings in the prior art, present invention solves the technical problem that being to provide a kind of push type magnetic bead transfer
Structure makes magnetic bead and is adsorbed in the material to be extracted of magnetic bead by the way that push type magnetic bead transfer device is arranged between adjacent cavity
(protein, nucleic acid) sufficiently, is efficiently shifted.
A kind of push type magnetic bead transfer device is provided present invention solves the technical problem that also residing in, not only self structure letter
It is single and easy to operate, the complexity of the matched equipment used can be effectively reduced.
In order to solve the above-mentioned technical problem, the reagent provided in an embodiment of the present invention for being provided with push type magnetic bead transfer device
Cartridge includes the first cavity being disposed adjacent and the second cavity and the interval that first cavity and the second cavity is spaced apart
Region；It is characterized by: forming an accommodating chamber in the interval region, one is provided with by the intracorporal magnetic of the first chamber in the accommodating chamber
Pearl is transferred to the intracorporal push type magnetic bead transfer device of the second chamber, and the push type magnetic bead transfer device sealing is embedded in the appearance
It receives intracavitary；
The push type magnetic bead transfer device includes rotor and the push rod for pushing the rotor rotation, and the rotor bottom is set
It is equipped with the cylindricality rotational structure driven by rotor；The rotor is provided with a through-hole along its central axes, is provided with spiral shell in the through-hole
It revolves shape and rotates slide, the outer surface of the push rod is provided with the spiral rotation protrusion to match with the spiral rotation slide；
The cylindricality rotational structure is set in the accommodating chamber and forms sealed spacer region for the first chamber with accommodating chamber
Body and the second cavity separate, and the joining place of the cylindricality rotational structure and rotor is provided with rotation linkage structure；It is pressed in push rod
When propulsion, rotor drives cylindricality rotational structure rotation that the intracorporal magnetic bead of the first chamber is transferred to the by rotation linkage structure
In two cavitys；When push rod is extracted or popped up outward, the linkage structure between rotor and cylindricality rotational structure fails, and rotor exists
Then revolution playback is separated under the drive of push rod with cylindricality rotational structure；
The side of the cylindricality rotational structure is provided with the groove for accommodating magnetic bead, is additionally provided with absorption in the interval region
The magnet of magnetic bead；For the groove in the magnetic bead transfer previous dynasty to first cavity, magnetic bead is adsorbed to the groove by the magnet
It is interior；When magnetic bead shifts, pressing push rod enters in the through-hole of rotor, and the spiral rotation protrusion of push rod acts on the helical form of through-hole
Rotation slide drive rotor rotates about the axis thereof, and rotor drives the cylindricality rotational structure in accommodating chamber by rotation linkage structure
Interior rotation, the magnetic bead adhered in the groove and groove are turned to towards in the region of the second cavity.
As a preferred solution of the present invention, the examination for being provided with push type magnetic bead transfer device that the embodiment of the present invention provides
Agent cartridge further comprises some or all of following technical characteristic:
Preferably, the cylindricality rotational structure is set as the H-shaped column construction of shaft section comprising upper groove and recessed
Slot and the linking area that upper groove, lower groove is separated, the rotor is in being connected connection with cylindricality rotational structure in the upper groove
It is dynamic.
Preferably, the rotation linkage structure is the square shaped teeth for being set to the rotor Yu cylindricality rotational structure, the side
The region that the bottom of the rotor is arranged in shape tooth and cylindricality rotational structure is mutually connected with the bottom of rotor；Positioned at the rotor
Linkage is realized in intermeshing linking when push rod is pressed and promoted of the square shaped teeth of bottom and cylindricality rotational structure, is extracted outward in push rod
Or it is separated from each other when pop-up.
Preferably, the magnet is installed and is controlled on the external equipment being used cooperatively with reagent cartridge, magnet setting
Enter lower groove in lower groove or when magnetic bead adsorbs and shifts.
Preferably, the magnet is permanent magnet or electromagnet.
Preferably, first cavity and the second cavity are arranged in parallel, in magnetic bead transfer process, the push rod drive rotor
Half revolution, the cylindricality rotational structure also rotate half cycle under the drive of rotor by rotating linkage structure.
Preferably, reagent cartridge of the invention further includes a upper cover, and the upper cover is set to push type magnetic bead transfer device
And the receiving cavity wall being mutually linked in the cylindricality rotational structure and the first cavity and the second cavity is suppressed, the upper Gai Shangkai
Equipped with the guide through hole for positioning the rotor, the push rod extends to rotor through the guide through hole.
Preferably, it is provided with a retention bead on the rotor, is additionally provided on the upper lid and the retention bead phase
Match and limit the detent area of rotor rotation half cycle.
Preferably, the retention bead is parallel to the slot bottom of the groove, and detent area is resisted against described before rotor rotation
Retention bead is to position the initial position of rotor, and retention bead is resisted against the detent area for covering the other side after rotor half revolution
To limit rotor and the equal half revolution of rotational parts.
In addition, including mutually rank in the reagent cartridge provided in an embodiment of the present invention for being provided with push type magnetic bead transfer device
Reagent cartridge described in the multiple above schemes connect.Push type magnetic bead transfer device i.e. provided in an embodiment of the present invention includes the
One, two, three ... ..n cavity is provided with push type magnetic bead transfer device between two adjacent cavitys.
Push type magnetic bead transfer device provided in an embodiment of the present invention, has the following beneficial effects:
The reagent cartridge provided in an embodiment of the present invention for being provided with push type magnetic bead transfer device is by being disposed adjacent
The side of push type magnetic bead transfer device between cavity, the cylindricality rotational structure in the push type magnetic bead transfer device is provided with appearance
Receive the groove of magnetic bead, in the magnetic bead transfer previous dynasty to first cavity, magnetic bead is adsorbed in the groove groove by magnet；Magnetic bead
When transfer, push rod presses the rotation of push belt turn, and rotor drives the cylindricality rotational structure rotation by rotation linkage structure
The intracorporal magnetic bead of first chamber is transferred in the second cavity, so that the magnetic bead for adhering to groove turns in the second cavity, thus
Realization magnetic bead and the material to be extracted (protein, nucleic acid) for being adsorbed in magnetic bead sufficiently, are efficiently shifted, to overcome core
In the extractions of the substances such as acid, albumen, detection process between each step due to the linkings operation such as material transfer existing for trivial operations,
The problem of sample or reagent vulnerable to pollution.In addition, push type magnetic bead transfer device of the invention, not only self structure is simple, and
And it is easy to operate, it, can be by push rod as long as push rod is pushed axially due to the ingehious design in push type magnetic bead transfer device
The magnetic bead that groove adheres to is turned in the second cavity, is being pushed away by the propelled longitudinally rotation for being converted to rotor and cylindricality rotational structure
When bar is extracted or popped up outward, the linkage structure between rotor and cylindricality rotational structure fails, and rotor is under the drive of push rod
Then revolution playback is separated with cylindricality rotational structure, is quickly and easily switched in next step or other are provided with push type magnetic
The reagent cartridge respective operations of pearl transfer device.Therefore, setting is not needed in the ancillary equipment of periphery to turn exclusively for magnetic bead
Fortune provides the rotating machinery of rotational power, it is only necessary to the power source of axial motive force is provided, thus significantly reduce with
The equipment being used cooperatively complexity；In addition, passing through setting spiral rotation slide and matched spiral rotation
Protrusion not only greatly improves transmission compared to existing screw-driven in conjunction with being used cooperatively for retention bead and detent area
Efficiency, and rotation radian is effectively limited in half cycle, to guarantee that the groove for shifting magnetic bead can be accurately positioned and rotate
Half cycle, to realize the abundant transfer of magnetic bead and magnetic bead absorption material.
Detailed description of the invention
Fig. 1 is that the structure for the reagent cartridge for being provided with push type magnetic bead transfer device that the preferred embodiment of the present invention provides is shown
It is intended to one.
Fig. 2 is cross-sectional view of the Fig. 1 in the direction A-A.
Fig. 3 is that the structure for the reagent cartridge for being provided with push type magnetic bead transfer device that the preferred embodiment of the present invention provides is shown
It is intended to two.
Fig. 4 is that the structure for the reagent cartridge for being provided with push type magnetic bead transfer device that the preferred embodiment of the present invention provides is shown
It is intended to three.
Fig. 5 is the structural schematic diagram of the cylindricality rotational structure for the reagent cartridge that the preferred embodiment of the present invention provides.
Fig. 6 is the structural schematic diagram of the rotor for the reagent cartridge that the preferred embodiment of the present invention provides.
Fig. 7 is the structural schematic diagram of the push rod for the reagent cartridge that the preferred embodiment of the present invention provides.
Specific embodiment
It includes: to be disposed adjacent that the embodiment of the present invention, which provides a kind of reagent cartridge for being provided with push type magnetic bead transfer device,
First cavity and the second cavity and the interval region that first cavity and the second cavity is spaced apart；Shape in the interval region
At an accommodating chamber, it is provided with one in the accommodating chamber, the intracorporal magnetic bead of the first chamber is transferred to the intracorporal push type magnetic bead of the second chamber
Transfer device, the push type magnetic bead transfer device sealing are embedded in the accommodating chamber；The push type magnetic bead transfer device
Including rotor and the push rod for pushing the rotor to rotate, the rotor bottom is provided with the cylindricality rotational structure driven by rotor；
The rotor is provided with a through-hole along its central axes, and spiral rotation slide is provided in the through-hole, and the outer surface of the push rod is set
It is equipped with the spiral rotation protrusion to match with the spiral rotation slide；The cylindricality rotational structure is set to the receiving
It is intracavitary and form sealed spacer region with accommodating chamber and separate the first cavity and the second cavity, the cylindricality rotational structure and rotor
Joining place be provided with rotation linkage structure；When push rod is pressed and promoted, rotor drives the cylindricality by rotation linkage structure
The intracorporal magnetic bead of first chamber is transferred in the second cavity by rotational structure rotation；When push rod is extracted or is popped up outward, rotor
Linkage structure failure between cylindricality rotational structure, rotor are separated with cylindricality rotational structure under the drive of push rod and are then turned round
Playback；The side of the cylindricality rotational structure is provided with the groove for accommodating magnetic bead, is additionally provided with absorption magnetic in the interval region
The magnet of pearl；In the magnetic bead transfer previous dynasty to first cavity, magnetic bead is adsorbed in the groove groove by the magnet；
When magnetic bead shifts, pressing push rod enters in the through-hole of rotor, and the spiral rotation protrusion of push rod acts on the helical form rotation of through-hole
Turn slide drive rotor to rotate about the axis thereof, rotor drives the cylindricality rotational structure in accommodating chamber by rotation linkage structure
It rotates, the magnetic bead adhered in the groove and groove is turned to towards in the region of the second cavity.
Wherein, the push type magnetic bead transfer device includes rotor and the push rod for pushing the rotor rotation, the rotor
Bottom is provided with the cylindricality rotational structure driven by rotor；The rotor is provided with a through-hole along its central axes, sets in the through-hole
It is equipped with spiral rotation slide, the outer surface of the push rod is provided with the spiral rotation to match with the spiral rotation slide
Protrusion；
The cylindricality rotational structure is set in the accommodating chamber and forms sealed spacer region for the first chamber with accommodating chamber
Body and the second cavity separate, and the joining place of the cylindricality rotational structure and rotor is provided with rotation linkage structure；It is pressed in push rod
When propulsion, rotor drives cylindricality rotational structure rotation that the intracorporal magnetic bead of the first chamber is transferred to the by rotation linkage structure
In two cavitys；When push rod is extracted or popped up outward, the linkage structure between rotor and cylindricality rotational structure fails, and rotor exists
Then revolution playback is separated under the drive of push rod with cylindricality rotational structure；
The side of the cylindricality rotational structure is provided with the groove for accommodating magnetic bead, is additionally provided with absorption in the interval region
The magnet of magnetic bead；For the groove in the magnetic bead transfer previous dynasty to first cavity, magnetic bead is adsorbed to the groove by the magnet
It is interior；When magnetic bead shifts, pressing push rod enters in the through-hole of rotor, and the spiral rotation protrusion of push rod acts on the helical form of through-hole
Rotation slide drive rotor rotates about the axis thereof, and rotor drives the cylindricality rotational structure in accommodating chamber by rotation linkage structure
Interior rotation, the magnetic bead adhered in the groove and groove are turned to towards in the region of the second cavity.
Wherein, the rotation linkage structure is the square shaped teeth for being set to the rotor Yu cylindricality rotational structure, described rectangular
The region that the bottom of the rotor is arranged in tooth and cylindricality rotational structure is mutually connected with the bottom of rotor；Positioned at the bottom of the rotor
Linkage is realized in intermeshing linking when push rod is pressed and promoted of the square shaped teeth of portion and cylindricality rotational structure, extracted outward in push rod or
Person is separated from each other when popping up.
Wherein, the magnet is installed and is controlled on the external equipment being used cooperatively with reagent cartridge, and magnet setting exists
Lower groove enters lower groove when magnetic bead adsorbs and shifts.
Wherein, the magnet is permanent magnet or electromagnet.
Wherein, first cavity and the second cavity are arranged in parallel, in magnetic bead transfer process, the push rod drive rotor rotation
Turn half cycle, the cylindricality rotational structure also rotates half cycle under the drive of rotor by rotating linkage structure.
Wherein, the push type magnetic bead transfer device further includes a upper cover, and the upper cover is set to the transfer of push type magnetic bead
The receiving cavity wall being mutually linked in the cylindricality rotational structure and the first cavity and the second cavity, the upper cover are simultaneously suppressed by mechanism
On offer the guide through hole for positioning the rotor, the push rod extends to rotor through the guide through hole.
Wherein, it is provided with a retention bead on the rotor, is additionally provided on the upper lid and the retention bead phase
Match and limit the detent area of rotor rotation half cycle.
In addition, including mutually rank in the reagent cartridge provided in an embodiment of the present invention for being provided with push type magnetic bead transfer device
Reagent cartridge described in the multiple above schemes connect.Push type magnetic bead transfer device i.e. provided in an embodiment of the present invention includes the
One, two, three ... ..n cavity is provided with push type magnetic bead transfer device between two adjacent cavitys.
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 1 and 2 is the structural schematic diagram for the push type magnetic bead transfer device that the preferred embodiment of the present invention provides.The push type
Magnetic bead transfer device is applied in nucleic acid or albumen preparation and detection device, it is intended in nucleic acid or albumen preparation and detection process
Material transfer between adjacent step provide it is a kind of efficiently, efficiently solution, but be not limited thereto, in practical application
In, the box that extracts can also be applied to the extraction of other biological extract.
As shown in figs. 1-7, the reagent cartridge for being provided with push type magnetic bead transfer device that the preferred embodiment of the present invention provides
Including the first cavity 10 and the second cavity 20 and the interval region 30 that the first cavity 10 and the second cavity 20 is spaced apart.Specifically
When realization, an accommodating chamber 31 is formed in the interval region 30, and one is provided in accommodating chamber 31 and turns the magnetic bead in the first cavity 10
The intracorporal push type magnetic bead transfer device 40 of the second chamber is moved to, the sealing of push type magnetic bead transfer device 40 is embedded in accommodating chamber
31.Push type magnetic bead transfer device 40 includes the push rod 43 that rotor 42 and drive rotor 42 rotate, 42 bottom of rotor be provided with by
The cylindricality rotational structure 41 that rotor drives.During specific implementation, cylindricality rotational structure 41 be set in accommodating chamber and with receiving
Chamber forms sealed spacer region and the first cavity 10 and the second cavity is separated 20, the joining place of cylindricality rotational structure 41 and rotor 42
It is provided with rotation linkage structure 424.When push rod 43 is pressed and promoted, rotor 42 drives cylindricality rotation knot by rotation linkage structure
Magnetic bead in first cavity 10 is transferred in the second cavity 20 by the rotation of structure 41；When push rod is extracted or is popped up outward, rotor
Linkage structure failure between 42 and cylindricality rotational structure 41, rotor 42 divide under the drive of push rod 43 with cylindricality rotational structure 41
From then revolution playback.
In order to facilitate propulsion is screwed during the pressing of push rod 43, its central axes of rotor 42 are provided with a through-hole 421, the through-hole
Interior 421 are provided with spiral rotation slide 422, and the outer surface of the push rod 43 is provided with to match with spiral rotation slide 422
Spiral rotation protrusion 431.When push rod 43 is longitudinally propelling along through-hole 421, pass through spiral rotation protrusion 431 and spiral
Shape rotates the interaction of slide 422, and 42 axial translation of push rod is converted into the rotation of rotor 42, and passes through rotation linkage structure
424 drive cylindricality rotational structures 41 rotate in accommodating chamber.
In order to sufficiently, efficiently shift magnetic bead, the side of cylindricality rotational structure 41 is provided with the groove for accommodating magnetic bead
413, the magnet (not shown) of absorption magnetic bead is additionally provided in interval region 30.It is, in principle, that the present invention mainly passes through
Using the magnetic-particle including magnetic bead including, as extraction nucleic acid, albumen or other macromolecular bio-extracts, magnetic bead is the
One cavity 10 realizes the suction-operated to nucleic acids in samples, albumen or other macromolecular bio-extracts, mixes in the first cavity 10
It closes and is adsorbed with nucleic acid to be extracted, albumen or other macromolecular bio-extracts on the magnetic bead in material.At this point, i.e. magnetic bead shifts
Before, magnetic bead is adsorbed in groove 413 by groove 413 towards the first cavity 10, magnet；Magnetic bead transfer when, pressing push rod 43 into
Enter in the through-hole 421 of rotor 42, the spiral rotation protrusion 431 of push rod 43 acts on the spiral rotation slide 422 of through-hole 421
Drive rotor 42 rotates about the axis thereof, and rotor 42 drives the cylindricality rotational structure in accommodating chamber 31 by rotation linkage structure
The magnetic bead accommodated in rotation, groove 413 and groove 413 is turned to towards in the region of the second cavity.It is tied by rotating cylindricality
The side local setting of structure 41 is groove 413, and makes groove 413 in the absorption previous dynasty to the first cavity 10, to magnetic bead in magnetic force
Under the action of be adsorbed on the groove 413, then accommodating chamber is rotated through by 42 drive rotor of push rod and cylindricality rotational structure 41,
It is capable of forming gap between groove 413 and the curved surface cavity wall of accommodating chamber during rotation, and the magnetic being adsorbed on groove 413
Pearl is because the presence of this gap falls off without being received the cavity wall of chamber and scraping off, to realize effectively material transfer, while column
It is matched in shape rotational structure 41 except other positions of groove 413 can be sealed with accommodating chamber 31, ensure that other in addition to magnetic bead
Mutual independence of the material between the first cavity and the second cavity.
When specific implementation, cylindricality rotational structure 41 is set as the H-shaped column construction of shaft section in the embodiment of the present invention,
Including upper groove 411 and lower groove 412 and the linking area 414 that upper groove, lower groove is separated.Wherein, rotor 42 is in fovea superior
It is connected linkage with cylindricality rotational structure 41 in slot 411.In this preferred embodiment, rotation linkage structure 424 be set to rotor with
The square shaped teeth of cylindricality rotational structure, the bottom of square shaped teeth setting rotor 42 and cylindricality rotational structure 41 and the bottom of rotor are mutually held in the mouth
The region connect；Positioned at the bottom of rotor and the square shaped teeth of cylindricality rotational structure, intermeshing linking when push rod is pressed and promoted is realized
Linkage, is separated from each other when push rod is extracted or popped up outward.In addition, magnet is installed and is controlled in be made with the cooperation of reagent cartridge
On external equipment, magnet setting enters lower groove 412 in lower groove or when magnetic bead adsorbs and shifts.It is shifted in magnetic bead
Before, towards the first cavity 10, magnetic bead concentrates in groove 413 groove 413 under the magneticaction that magnet generates；Turn in magnetic bead
When shifting, pressing push rod 43 enters in the through-hole 421 of rotor 42, and the spiral rotation protrusion 431 of push rod 43 acts on through-hole 421
422 drive rotor 42 of spiral rotation slide rotates about the axis thereof, and rotor 42 drives the cylindricality to turn by rotation linkage structure
Dynamic structure rotates in accommodating chamber 31, and the magnetic bead accommodated in groove 413 and groove 413 turns to the region towards the second cavity
It is interior.For magnet selection with from the point of view of material, when specific implementation, magnet is permanent magnet or electromagnet.In implementation of the invention
Permanent magnet or can be extraordinary preferred embodiment by the electromagnet of power control is selected in example.
In a preferred embodiment of the invention, the first cavity 10 and the second cavity 20 are arranged in parallel, therefore, magnetic bead transfer
When, 43 drive rotor half revolution of push rod, correspondingly, cylindricality rotational structure 41 is by rotation linkage structure 424 in rotor
Half cycle is also rotated under 42 drive.Certainly in other embodiments of the present invention, the first cavity 10 and the second cavity 20 can be with
There are certain angles for not parallel setting and the two, and at this point, push rod 42 promotes size and 41 angle of cylindricality rotational structure also to lead to
The angle crossed between the first cavity 10 and the second cavity 20 is determined, thus enable magnetic bead in the first cavity 10 it is accurate,
It is efficiently transferred in the second cavity.
In addition, reagent cartridge of the invention further includes a upper cover 50.As shown in Figure 3,4, upper cover 50 is set to outside push rod 43
It encloses and suppresses the receiving cavity wall being mutually linked in the cylindricality rotational structure and the first cavity 10 and the second cavity 20, upper cover 50
On offer the guide through hole 431 for positioning the push rod, push rod 42 extends to cylindricality rotational structure 41 through guide through hole 431,
To play to the good fixation of push rod 42 and guiding role.
In addition, to be accurately defined the initial position of rotor 42, cylindricality rotational structure 41, it is ensured that groove can turn in magnetic bead
The previous dynasty is moved to the first cavity 10, alsos for guaranteeing that rotor 42 and cylindricality rotational structure 41 are accurately selected in push rod pressing progradation
It selects half cycle and is ultimately towards the second cavity 20, a retention bead 423 is provided on rotor 42, is additionally provided with and limits in upper cover 50
Protrusion 423 matches and limits the detent area (not shown) of rotor rotation half cycle.Retention bead 423 is parallel to the slot of groove
Bottom, detent area are resisted against retention bead before rotor rotation to position the initial position of rotor, to limit rotor 42, cylindricality turns
The initial position of dynamic structure 41, it is ensured that groove can shift the previous dynasty to the first cavity 10 in magnetic bead；It is limited after rotor half revolution
Protrusion is resisted against the detent area for covering the other side, to guarantee rotor 42 and cylindricality rotational structure in push rod pressing progradation
41 accurately select half cycle and are ultimately towards the second cavity 20.
It should be noted that structure can be seen that and be provided with push type provided in an embodiment of the present invention from Fig. 1 and 2
The reagent cartridge of magnetic bead transfer device only includes 20 two cavitys being disposed adjacent of the first cavity 10 and the second cavity.In fact,
Using only there are two the reagent cartridges of cavity as a kind of preferred embodiment of the present invention program in the preferred embodiment of the present invention, it is
In order to start with from simple structure, illustrate that the present invention is the material in nucleic acid or albumen preparation and detection process between adjacent step
One kind that transfer provides efficiently, efficiently solution, but be not limited thereto, in practical applications, due to nucleic acid extraction with
And it is related to the material transfer of multiple steps in detection process, have the material transfer step for being related to realizing by magnetic bead
It is multiple, it can also include in other embodiments of the invention in the multiple above schemes being mutually linked in the reagent cartridge therefore
The reagent cartridge.Push type magnetic bead transfer device i.e. provided in an embodiment of the present invention include first, second and third ... ..n
Cavity is provided with push type magnetic bead transfer device between two adjacent cavitys.
The reagent cartridge for being provided with push type magnetic bead transfer device that above-described embodiment discloses passes through in the chamber being disposed adjacent
The side of push type magnetic bead transfer device between body, the cylindricality rotational structure in the push type magnetic bead transfer device is provided with receiving
The groove of magnetic bead, in the magnetic bead transfer previous dynasty to first cavity, magnetic bead is adsorbed in the groove groove by magnet；Magnetic bead turns
When shifting, push rod presses the rotation of push belt turn, and rotor drives the cylindricality rotational structure rotation will by rotation linkage structure
The intracorporal magnetic bead of first chamber is transferred in the second cavity, so that the magnetic bead for adhering to groove turns in the second cavity, thus real
Existing magnetic bead and the material to be extracted (protein, nucleic acid) for being adsorbed in magnetic bead sufficiently, are efficiently shifted, thus overcome nucleic acid,
In the extractions of the substances such as albumen, detection process between each step due to the linkings operation such as material transfer existing for trivial operations, sample
The problem of product or reagent vulnerable to pollution.In addition, push type magnetic bead transfer device of the invention, not only self structure is simple, but also
It is easy to operate, it, can be by the vertical of push rod as long as push rod is pushed axially due to the ingehious design in push type magnetic bead transfer device
It is converted to the rotation of rotor and cylindricality rotational structure to promotion, the magnetic bead that groove adheres to is turned in the second cavity, in push rod
Outward extract or pop-up when, between rotor and cylindricality rotational structure linkage structure failure, rotor under the drive of push rod with
Cylindricality rotational structure separation then revolution playback, is quickly and easily switched in next step or other is provided with push type magnetic bead
The reagent cartridge respective operations of transfer device.Therefore, setting is not needed in the ancillary equipment of periphery to transport exclusively for magnetic bead
There is provided the rotating machinery of rotational power, it is only necessary to the power source of axial motive force is provided, to significantly reduce therewith
The complexity for the equipment being used cooperatively；In addition, convex by setting spiral rotation slide and matched spiral rotation
It rises, in conjunction with being used cooperatively for retention bead and detent area, not only greatly improves transmission effect compared to existing screw-driven
Rate, and rotation radian is effectively limited in half cycle, to guarantee that the groove for shifting magnetic bead can be accurately positioned and rotate half
Week, to realize the abundant transfer of magnetic bead and magnetic bead absorption material.
The above is a preferred embodiment of the present invention, it is noted that for those skilled in the art
For, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also considered as
Protection scope of the present invention.
Claims (7)
1. a kind of reagent cartridge for being provided with push type magnetic bead transfer device, which includes the first cavity being disposed adjacent
With the second cavity and the interval region that first cavity and the second cavity is spaced apart；It is characterized by: the interval region
One accommodating chamber of interior formation is provided with one in the accommodating chamber and the intracorporal magnetic bead of the first chamber is transferred to the intracorporal push type of the second chamber
Magnetic bead transfer device, the push type magnetic bead transfer device sealing are embedded in the accommodating chamber；
The push type magnetic bead transfer device includes rotor and the push rod for pushing the rotor rotation, and the rotor bottom is provided with
The cylindricality rotational structure driven by rotor；The rotor is provided with a through-hole along its central axes, is provided with helical form in the through-hole
Slide is rotated, the outer surface of the push rod is provided with the spiral rotation protrusion to match with the spiral rotation slide；
The cylindricality rotational structure be set in the accommodating chamber and with accommodating chamber formed sealed spacer region by the first cavity and
Second cavity separates, and the joining place of the cylindricality rotational structure and rotor is provided with rotation linkage structure；It presses and promotes in push rod
When, rotor drives the cylindricality rotational structure rotation that the intracorporal magnetic bead of the first chamber is transferred to the second chamber by rotation linkage structure
In vivo；When push rod is extracted or popped up outward, the linkage structure between rotor and cylindricality rotational structure fails, and rotor is in push rod
Drive under separated with cylindricality rotational structure then revolution playback；
The side of the cylindricality rotational structure is provided with the groove for accommodating magnetic bead, is additionally provided with absorption magnetic bead in the interval region
Magnet；In the magnetic bead transfer previous dynasty to first cavity, magnetic bead is adsorbed in the groove groove by the magnet；Magnetic
When pearl shifts, pressing push rod enters in the through-hole of rotor, and the spiral rotation protrusion of push rod acts on the spiral rotation of through-hole
Slide drive rotor rotates about the axis thereof, and rotor drives the cylindricality rotational structure to turn in accommodating chamber by rotation linkage structure
Dynamic, the magnetic bead adhered in the groove and groove is turned to towards in the region of the second cavity；
The cylindricality rotational structure is set as the H-shaped column construction of shaft section comprising upper groove and lower groove and will be upper
The separated linking area of groove, lower groove, the rotor is in being connected linkage with cylindricality rotational structure in the upper groove；
The rotation linkage structure is the square shaped teeth for being set to the rotor Yu cylindricality rotational structure, described in the square shaped teeth setting
The region that the bottom of rotor and cylindricality rotational structure are mutually connected with the bottom of rotor；Turn positioned at the bottom of the rotor and cylindricality
Linkage is realized in intermeshing linking when push rod is pressed and promoted of the square shaped teeth of dynamic structure, extracts or pop up outward phase in push rod
Mutually separation；
The magnet, which is installed, to be simultaneously controlled on the external equipment being used cooperatively with reagent cartridge, magnet setting in lower groove or
Enter lower groove when magnetic bead adsorbs and shifts.
2. being provided with the reagent cartridge of push type magnetic bead transfer device as described in claim 1, it is characterised in that: the magnet
For permanent magnet or electromagnet.
3. being provided with the reagent cartridge of push type magnetic bead transfer device as described in claim 1, it is characterised in that: described first
Cavity and the second cavity are arranged in parallel, in magnetic bead transfer process, the push rod drive rotor half revolution, and the cylindricality rotation knot
Structure also rotates half cycle under the drive of rotor by rotating linkage structure.
4. being provided with the reagent cartridge of push type magnetic bead transfer device as described in claim 1, it is characterised in that: further include one
Upper cover, the upper cover are set to push type magnetic bead transfer device and suppress in the cylindricality rotational structure and the first cavity and second
The receiving cavity wall that cavity is mutually linked offers the guide through hole for positioning the rotor on the upper lid, and the push rod runs through
The guide through hole extends to rotor.
5. being provided with the reagent cartridge of push type magnetic bead transfer device as claimed in claim 4, it is characterised in that: the rotor
On be provided with a retention bead, be additionally provided on the upper lid and rotor rotation half cycle matched and limited with the retention bead
Detent area.
6. being provided with the reagent cartridge of push type magnetic bead transfer device as claimed in claim 5, it is characterised in that: the limit
Projecting parallel is resisted against the retention bead before rotor rotation in the slot bottom of the groove, detent area to position the initial of rotor
Position, retention bead is resisted against the detent area for covering the other side and is revolved with limiting rotor and rotational parts after rotor half revolution
Turn half cycle.
7. a kind of reagent cartridge for being provided with push type magnetic bead transfer device, it is characterised in that: comprising mutual in the reagent cartridge
Multiple reagent cartridges as described in any one of claim 1-6 of linking.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610329938.7A CN105950438B (en) | 2016-05-17 | 2016-05-17 | A kind of reagent cartridge being provided with push type magnetic bead transfer device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610329938.7A CN105950438B (en) | 2016-05-17 | 2016-05-17 | A kind of reagent cartridge being provided with push type magnetic bead transfer device |
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| Publication Number | Publication Date |
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| CN105950438A CN105950438A (en) | 2016-09-21 |
| CN105950438B true CN105950438B (en) | 2019-08-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610329938.7A Active CN105950438B (en) | 2016-05-17 | 2016-05-17 | A kind of reagent cartridge being provided with push type magnetic bead transfer device |
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| CN113460465A (en) * | 2020-06-30 | 2021-10-01 | 盛振华 | Reagent kit for reagent detection |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204607980U (en) * | 2015-04-10 | 2015-09-02 | 余家昌 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
| CN204608019U (en) * | 2015-04-09 | 2015-09-02 | 余家昌 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
| CN204832194U (en) * | 2015-07-03 | 2015-12-02 | 艾康生物技术(杭州)有限公司 | Biological sample testing cassette |
| CN205188260U (en) * | 2015-11-13 | 2016-04-27 | 余家昌 | Reagent card box of liquid level slope when material shifts |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9050594B2 (en) * | 2012-02-13 | 2015-06-09 | Neumodx Molecular, Inc. | System and method for processing and detecting nucleic acids |
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2016
- 2016-05-17 CN CN201610329938.7A patent/CN105950438B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204608019U (en) * | 2015-04-09 | 2015-09-02 | 余家昌 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
| CN204607980U (en) * | 2015-04-10 | 2015-09-02 | 余家昌 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
| CN204832194U (en) * | 2015-07-03 | 2015-12-02 | 艾康生物技术(杭州)有限公司 | Biological sample testing cassette |
| CN205188260U (en) * | 2015-11-13 | 2016-04-27 | 余家昌 | Reagent card box of liquid level slope when material shifts |
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| CN105950438A (en) | 2016-09-21 |
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Effective date of registration: 20170526 Address after: 200120 Shanghai Pudong New Area city wide 222 Lane 6, 5 Dan Applicant after: Bio Technology (Shanghai) Co., Ltd. Address before: 361, Ruth Town, San Francisco, Calif. Applicant before: Yu Jiachang Applicant before: Ai Jiesi bio tech ltd Applicant before: Bio Technology (Shanghai) Co., Ltd. |
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