CN105950438A - Reagent card box provided with pressing-type magnetic bead transfer mechanism - Google Patents
Reagent card box provided with pressing-type magnetic bead transfer mechanism Download PDFInfo
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- CN105950438A CN105950438A CN201610329938.7A CN201610329938A CN105950438A CN 105950438 A CN105950438 A CN 105950438A CN 201610329938 A CN201610329938 A CN 201610329938A CN 105950438 A CN105950438 A CN 105950438A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The invention provides a reagent card box provided with a pressing-type magnetic bead transfer mechanism. The reagent card box comprises a first cavity, a second cavity, and a partitioning area for partitioning the first cavity and the second cavity, wherein an accommodating cavity is formed in the partitioning area, the interior of the accommodating cavity is provided with a pressing-type magnetic bead transfer mechanism used for transferring magnetic beads in the first cavity into the second cavity, the pressing-type magnetic bead transfer mechanism is embedded in the accommodating cavity in a sealed manner, and comprises a rotor and a push rod for pushing the rotor to rotate, and a cylinder-shaped rotation structure driven by the rotor is arranged on the bottom of the rotor. According to the scheme, the pressing-type magnetic bead transfer mechanism is arranged between the adjacent cavities, so that the magnetic beads and to-be-extracted materials adsorbed to the magnetic beads are sufficiently and efficiently transferred. In addition, the reagent card box is simple in structure and convenient to operate, and the complexity of a device matched with the reagent card box for use can be effectively reduced.
Description
Technical field
The present invention relates to preparation and the consumptive material used by detection of nucleic acid or protein, particularly relate to one and be provided with
The reagent cartridge of pressing type magnetic bead transfer device.
Background technology
Along with transformation and the development of personalized medicine of medical model, medical test circle in the urgent need to quickly,
Accurate detection means, Molecular Detection has then given play to the advantage of uniqueness.The basis of Molecular Detection is that analysis is tested
The histiocyte of survey person, hair, anticoagulation or blood stasis mark, and formaldehyde is fixed, in paraffin-embedded tissue
Gene and expression product, by complete on molecular level nucleic acid (DNA and RNA) detect, in disease
Before once occurring symptom, sign and biochemical change the most not yet occur, just can accurately make detection.
At present, molecular detection technology mainly has making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biological core
Chip technology etc..Molecular Detection product is mainly used in the clinical departments such as tumor, infection, heredity, Prenatal Screening
Detection, and MEC, technical service center, third party testing agency and microbial rapid detection city
The aspects such as field.Currently, the measuring means such as blood routine, cytology, pathology and immunology is all towards automatically
Change, integration, standardization direction are developed, but due to himself technical complexity of Molecular Detection, " from sample to
Result " full-automatic instrument platform few or there is many insoluble technical problems.Such as, exist
The centrifugal column method or the paramagnetic particle method that use in biological technical field carry out nucleic acid extraction, it is generally required to carry out cracking,
In conjunction with four steps such as, rinsing, eluting, add follow-up making nucleic acid molecular hybridization, polymerase chain reaction (PCR)
With detecting steps such as biochips, the full-automatic instrument of whole " from sample to result " is made to be very difficult to reality
Existing, single for the transfer of effective ingredient in each step, the modes using manual transfer in prior art more,
I.e. by the way of artificial by raw material or intermediate product at step successively and the reaction vessel that realizes step successively
In shift, it is therefore apparent that in prior art use manual material branch mode, not only complex operation,
Waste time and energy.What is more also resides in whole operating process and easily causes pollution, affects the purity of extract,
And material is difficult to fully, shifts efficiently, affect experimental result.Therefore, in view of Molecular Detection its from
Body technical complexity, the full-automatic instrument platform of " from sample to result " is few or there is many being difficult to and solves
Technical problem certainly, will realize abundant, the transfer efficiently of material between each step and be accomplished by consumptive material and reality
That tests equipment structurally and functionally carries out many considering, and can avoid such as becoming present in prior art
The problems such as this height, poor sensitivity, structure complexity, operation and associated assay devices are excessively complicated.
Summary of the invention
For deficiency of the prior art, present invention solves the technical problem that and be to provide a kind of pressing type magnetic bead
Transfer device, by arranging pressing type magnetic bead transfer device between adjacent cavity, makes magnetic bead and is adsorbed in
The material to be extracted (protein, nucleic acid) of magnetic bead is fully, shift efficiently.
Present invention solves the technical problem that and a kind of pressing type magnetic bead transfer device of offer, not only self knot are be provided
Structure is simple, and easy to operate, it is possible to be effectively reduced the complexity of the equipment of matched use.
In order to solve above-mentioned technical problem, what the embodiment of the present invention provided is provided with pressing type magnetic bead transfer device
Reagent cartridge include the first cavity being disposed adjacent and the second cavity and by described first cavity and the second chamber
The interval region that body is spaced apart；It is characterized in that: in this interval region, form a receiving chamber, described receiving chamber
Inside it is provided with a pressing type magnetic bead transfer device being transferred in the second cavity by the magnetic bead in the first cavity, institute
State the sealing of pressing type magnetic bead transfer device and be embedded in described receiving intracavity；
Described pressing type magnetic bead transfer device includes rotor and the push rod promoting described rotor to rotate, described rotor
Bottom is provided with the cylindricality rotational structure driven by rotor；Described rotor is provided with a through hole along its axis,
Being provided with helical form in this through hole and rotate sliding limit, the outer surface of this push rod is provided with and rotates sliding with described helical form
While the helical form matched rotates projection；
Described cylindricality rotational structure is arranged at described receiving intracavity and forms sealed spacer region by the with accommodating chamber
One cavity and the second cavity separate, and the joining place of described cylindricality rotational structure and rotor is provided with rotation linkage knot
Structure；When push rod pressing advances, rotor will by rotating linkage structure drive described cylindricality rotational structure rotation
Magnetic bead in first cavity is transferred in the second cavity；When push rod is outwards extracted or ejects, rotor and post
Linkage structure between shape rotational structure lost efficacy, rotor separate with cylindricality rotational structure under the drive of push rod and
Rear revolution playback；
The side of described cylindricality rotational structure is provided with the groove accommodating magnetic bead, also sets up in described interval region
There is the magnet of absorption magnetic bead；Described groove magnetic bead transfer the previous dynasty to described first cavity, described magnet is by magnetic
Pearl is adsorbed in described groove；During magnetic bead transfer, pressing push rod enters in the through hole of rotor, the spiral of push rod
Shape rotates the protruding helical form rotation sliding limit drive rotor acting on through hole and rotates about the axis thereof, and rotor is by rotation
Turning linkage structure drives described cylindricality rotational structure accommodating intracavity rotation, attachment in described groove and groove
Magnetic bead turns in the region of the second cavity.
As the preferred version of the present invention, what embodiments of the invention provided is provided with pressing type magnetic bead transfer
The reagent cartridge of structure farther includes the part or all of of techniques below feature:
Preferably, described cylindricality rotational structure is set to the column construction that shaft section is H-shaped, and it includes fovea superior
Groove and low groove and by upper groove, low groove separate linking district, described rotor in described upper groove with
The linking linkage of cylindricality rotational structure.
Preferably, described rotation linkage structure is the square shaped teeth being arranged at described rotor with cylindricality rotational structure,
Described square shaped teeth arranges the bottom of described rotor, the region being connected mutually with the bottom of rotor with cylindricality rotational structure；
It is positioned at the bottom of described rotor and the square shaped teeth of cylindricality rotational structure and engages each other linking when push rod pressing advances
Realize linkage, be separated from each other when push rod is outwards extracted or ejects.
Preferably, described magnet install and controlled be formed on reagent cartridge with the use of external equipment on, magnetic
Body is arranged on low groove or enters low groove when magnetic bead absorption and transfer.
Preferably, described magnet is permanent magnet or electromagnet.
Preferably, described first cavity and the second cavity be arranged in parallel, in magnetic bead transfer process, and described push rod
Drive rotor half revolution, described cylindricality rotational structure also turns under the drive of rotor by rotating linkage structure
Dynamic half cycle.
Preferably, the reagent cartridge of the present invention also includes that a upper cover, described upper cover are arranged at pressing type magnetic bead and turn
Moving mechanism also suppresses the receiving intracavity being mutually linked in described cylindricality rotational structure and the first cavity and the second cavity
Wall, described on cover and offer the guide through hole positioning described rotor, described push rod runs through described guide through hole
Extend to rotor.
Preferably, described rotor is provided with a spacing preiection, described on cover be additionally provided with spacing with described
Projection matches and limits rotor and rotates the screens district of half cycle.
Preferably, described spacing preiection is parallel to the bottom land of described groove, screens district rotor rotate before against
In described spacing preiection to position the initial position of rotor, after rotor half revolution, spacing preiection is resisted against upper cover
The screens district of upper opposite side is to limit rotor and the equal half revolution of rotational parts.
It addition, the reagent cartridge being provided with pressing type magnetic bead transfer device of embodiment of the present invention offer comprises
The reagent cartridge described in multiple such schemes being mutually linked.The pressing type magnetic that i.e. embodiment of the present invention provides
Pearl transfer device includes first, second and third ... ..n cavity, is provided with pressing type magnetic between two adjacent cavitys
Pearl transfer device.
The pressing type magnetic bead transfer device that the embodiment of the present invention provides, has the advantages that
The reagent cartridge being provided with pressing type magnetic bead transfer device that the embodiment of the present invention provides is by setting adjacent
Pressing type magnetic bead transfer device between the cavity put, the cylindricality rotational structure in this pressing type magnetic bead transfer device
Side be provided with the groove accommodating magnetic bead, groove is in magnetic bead transfer previous dynasty to described first cavity, and magnet will
Magnetic bead is adsorbed in described groove；During magnetic bead transfer, push rod pressing advances rotor driven to rotate, and rotor passes through
Rotating linkage structure drives the rotation of described cylindricality rotational structure that the magnetic bead in the first cavity is transferred to the second cavity
In, so that the magnetic bead of groove attachment turns in the second cavity, thus realize magnetic bead and be adsorbed in magnetic bead
Material to be extracted (protein, nucleic acid) fully, shift efficiently, thus overcomes nucleic acid, albumen etc.
The trivial operations that exists because linking operations such as material transfers between each step during the extraction of material, detection,
Sample or the problem of reagent vulnerable to pollution.It addition, the pressing type magnetic bead transfer device of the present invention, not only self
Simple in construction, and easy to operate, due to the ingehious design in pressing type magnetic bead transfer device, as long as along axle
To promoting push rod, can be by the propelled longitudinally rotation changing into rotor and cylindricality rotational structure of push rod, by recessed
The magnetic bead of groove attachment turns in the second cavity, and when push rod is outwards extracted or ejects, rotor turns with cylindricality
Linkage structure between dynamic structure lost efficacy, and rotor separates with cylindricality rotational structure under the drive of push rod and then returns
Lapse to position, be switched to quickly and easily next step or other be provided with the examination of pressing type magnetic bead transfer device
Agent cartridge respective operations.Therefore, it is not required to arrange in peripheral auxiliary equipment carry exclusively for magnetic bead transhipment
Rotating machinery for rotational power, it is only necessary to the power source of axial motive force is provided, thus effectively drops
The complexity of the equipment of low matched use；It addition, rotate sliding limit and phase therewith by arranging helical form
The helical form of coupling rotates projection, in conjunction with spacing preiection and screens district with the use of, not only compared to existing
Screw-driven greatly improve transmission efficiency, and effectively rotation radian is limited in half cycle, thus
Ensure that the groove of transfer magnetic bead can be accurately positioned and half revolution, thus realize magnetic bead and magnetic bead absorption material
Abundant transfer.
Accompanying drawing explanation
Fig. 1 is the reagent cartridge being provided with pressing type magnetic bead transfer device that the preferred embodiment of the present invention provides
Structural representation one.
Fig. 2 is the Fig. 1 sectional view in A-A direction.
Fig. 3 is the reagent cartridge being provided with pressing type magnetic bead transfer device that the preferred embodiment of the present invention provides
Structural representation two.
Fig. 4 is the reagent cartridge being provided with pressing type magnetic bead transfer device that the preferred embodiment of the present invention provides
Structural representation three.
Fig. 5 is the structural representation of the cylindricality rotational structure of the reagent cartridge that the preferred embodiment of the present invention provides.
Fig. 6 is the structural representation of the rotor of the reagent cartridge that the preferred embodiment of the present invention provides.
Fig. 7 is the structural representation of the push rod of the reagent cartridge that the preferred embodiment of the present invention provides.
Detailed description of the invention
The embodiment of the present invention provides a kind of reagent cartridge being provided with pressing type magnetic bead transfer device to include: adjacent
The first cavity arranged and the second cavity and by spacer spaced apart to described first cavity and the second cavity
Territory；Forming a receiving chamber in this interval region, described receiving intracavity is provided with one by the magnetic bead in the first cavity
The pressing type magnetic bead transfer device being transferred in the second cavity, described pressing type magnetic bead transfer device seals chimeric
In described receiving intracavity；Described pressing type magnetic bead transfer device includes rotor and promotes pushing away of described rotor rotation
Bar, described rotor bottom is provided with the cylindricality rotational structure driven by rotor；Described rotor sets along its axis
Being equipped with a through hole, be provided with helical form and rotate sliding limit in this through hole, the outer surface of this push rod is provided with described
Helical form rotates the helical form rotation projection that sliding limit matches；Described cylindricality rotational structure is arranged at described receiving
First cavity and the second cavity are also separated by intracavity with accommodating formation sealed spacer region, chamber, and described cylindricality rotates
The joining place of structure and rotor is provided with rotation linkage structure；When push rod pressing advances, rotor is by rotating
Linkage structure drives described cylindricality rotational structure to rotate and is transferred in the second cavity by the magnetic bead in the first cavity；
When push rod is outwards extracted or ejects, the linkage structure between rotor and cylindricality rotational structure lost efficacy, rotor
Separate with cylindricality rotational structure under the drive of push rod and then turn round playback；The side of described cylindricality rotational structure
It is provided with the groove accommodating magnetic bead, in described interval region, is additionally provided with the magnet of absorption magnetic bead；Described groove
To described first cavity, described magnet, magnetic bead was adsorbed in described groove the previous dynasty in magnetic bead transfer；Magnetic bead turns
During shifting, pressing push rod enters in the through hole of rotor, and the helical form of push rod rotates projection and acts on the spiral of through hole
Shape rotates sliding limit drive rotor and rotates about the axis thereof, and rotor drives described cylindricality to rotate by rotating linkage structure
Structure is accommodating intracavity rotation, and in described groove and groove, the magnetic bead of attachment turns to the district towards the second cavity
In territory.
Wherein, described pressing type magnetic bead transfer device includes rotor and the push rod promoting described rotor to rotate, institute
State rotor bottom and be provided with the cylindricality rotational structure driven by rotor；Described rotor is provided with one along its axis
Through hole, is provided with helical form and rotates sliding limit in this through hole, the outer surface of this push rod is provided with and described helical form
Rotate the helical form rotation projection that sliding limit matches；
Described cylindricality rotational structure is arranged at described receiving intracavity and forms sealed spacer region by the with accommodating chamber
One cavity and the second cavity separate, and the joining place of described cylindricality rotational structure and rotor is provided with rotation linkage knot
Structure；When push rod pressing advances, rotor will by rotating linkage structure drive described cylindricality rotational structure rotation
Magnetic bead in first cavity is transferred in the second cavity；When push rod is outwards extracted or ejects, rotor and post
Linkage structure between shape rotational structure lost efficacy, rotor separate with cylindricality rotational structure under the drive of push rod and
Rear revolution playback；
The side of described cylindricality rotational structure is provided with the groove accommodating magnetic bead, also sets up in described interval region
There is the magnet of absorption magnetic bead；Described groove magnetic bead transfer the previous dynasty to described first cavity, described magnet is by magnetic
Pearl is adsorbed in described groove；During magnetic bead transfer, pressing push rod enters in the through hole of rotor, the spiral of push rod
Shape rotates the protruding helical form rotation sliding limit drive rotor acting on through hole and rotates about the axis thereof, and rotor is by rotation
Turning linkage structure drives described cylindricality rotational structure accommodating intracavity rotation, attachment in described groove and groove
Magnetic bead turns in the region of the second cavity.
Wherein, described rotation linkage structure is the square shaped teeth being arranged at described rotor with cylindricality rotational structure, institute
State square shaped teeth and the bottom of described rotor is set, the region being connected mutually with the bottom of rotor with cylindricality rotational structure；
It is positioned at the bottom of described rotor and the square shaped teeth of cylindricality rotational structure and engages each other linking when push rod pressing advances
Realize linkage, be separated from each other when push rod is outwards extracted or ejects.
Wherein, described magnet install and controlled be formed on reagent cartridge with the use of external equipment on, magnet
It is arranged on low groove or enters low groove when magnetic bead absorption and transfer.
Wherein, described magnet is permanent magnet or electromagnet.
Wherein, described first cavity and the second cavity be arranged in parallel, and in magnetic bead transfer process, described push rod pushes away
Dynamic rotor half revolution, described cylindricality rotational structure also rotates under the drive of rotor by rotating linkage structure
Half cycle.
Wherein, described pressing type magnetic bead transfer device also includes that a upper cover, described upper cover are arranged at pressing type magnetic
Pearl transfer device also suppresses the receiving being mutually linked in described cylindricality rotational structure and the first cavity and the second cavity
Cavity wall, described on cover and offer the guide through hole positioning described rotor, described push rod runs through described guiding
Through hole extends to rotor.
Wherein, described rotor is provided with a spacing preiection, described on cover and be additionally provided with and described limit convex
Act the screens district matching and limiting rotor rotation half cycle.
It addition, the reagent cartridge being provided with pressing type magnetic bead transfer device of embodiment of the present invention offer comprises
The reagent cartridge described in multiple such schemes being mutually linked.The pressing type magnetic that i.e. embodiment of the present invention provides
Pearl transfer device includes first, second and third ... ..n cavity, is provided with pressing type magnetic between two adjacent cavitys
Pearl transfer device.
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clearly
Chu, be fully described by, it is clear that described embodiment be only a part of embodiment of the present invention rather than
Whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not making creation
The every other embodiment obtained under property work premise, broadly falls into the scope of protection of the invention.
The structural representation of the pressing type magnetic bead transfer device that Fig. 1 and 2 provides for the preferred embodiment of the present invention.
This pressing type magnetic bead transfer device is applied in nucleic acid or albumen preparation and detection equipment, it is intended to for nucleic acid or egg
During white preparation and detection material transfer between adjacent step provide one efficiently, solution efficiently,
But being not limited thereto, in actual applications, described extraction box also apply be applicable to carrying of other biological extract
Take.
As shown in figs. 1-7, the reagent being provided with pressing type magnetic bead transfer device that the preferred embodiment of the present invention provides
Cartridge includes the first cavity 10 and the second cavity 20 and by spaced apart to the first cavity 10 and the second cavity 20
Interval region 30.When implementing, form a receiving chamber 31 in this interval region 30, accommodate in chamber 31
It is provided with a pressing type magnetic bead transfer device 40 being transferred in the second cavity by the magnetic bead in the first cavity 10,
Pressing type magnetic bead transfer device 40 seals and is embedded in receiving intracavity 31.Pressing type magnetic bead transfer device 40 includes
The push rod 43 that rotor 42 and drive rotor 42 rotate, is provided with the cylindricality driven by rotor and turns bottom rotor 42
Dynamic structure 41.During implementing, cylindricality rotational structure 41 is arranged at receiving intracavity and is formed with accommodating chamber
First cavity 10 and the second cavity are separated 20 by sealed spacer region, cylindricality rotational structure 41 and rotor 42
Joining place is provided with rotation linkage structure 424.When push rod 43 pressing advances, rotor 42 is linked by rotation
Structure drives cylindricality rotational structure 41 to rotate and is transferred in the second cavity 20 by the magnetic bead in the first cavity 10；
When push rod is outwards extracted or ejects, the linkage structure between rotor 42 and cylindricality rotational structure 41 lost efficacy,
Rotor 42 separates with cylindricality rotational structure 41 under the drive of push rod 43 and then turns round playback.
Screwing propelling during push rod 43 pressing for convenience, its axis of rotor 42 is provided with a through hole 421,
In this through hole, 421 are provided with the sliding limit 422 of helical form rotation, and the outer surface of this push rod 43 is provided with and helical form
Rotate the helical form rotation protruding 431 that sliding limit 422 matches.When push rod 43 is longitudinally propelling along through hole 421
Time, the interaction on protruding 431 limits 422 sliding with helical form rotation is rotated by helical form, push rod 42 is axial
Translation is converted into the rotation of rotor 42, and drives cylindricality rotational structure 41 to exist by rotating linkage structure 424
Accommodate intracavity to rotate.
In order to shift magnetic bead fully, efficiently, the side of cylindricality rotational structure 41 is provided with receiving magnetic bead
Groove 413, be additionally provided with in interval region 30 absorption magnetic bead magnet (not shown).It is, in principle, that
The present invention mainly by use the magnetic-particle including magnetic bead as extract nucleic acid, albumen or other
Macromole bio-extract, magnetic bead realizes nucleic acids in samples, albumen or other macromole at the first cavity 10
The adsorption of bio-extract, is adsorbed with core to be extracted on the magnetic bead in mixed material in the first cavity 10
Acid, albumen or other macromole bio-extracts.Now, i.e. before magnetic bead transfer, groove 413 is towards first
Cavity 10, magnetic bead is adsorbed in groove 413 by magnet；When magnetic bead shifts, pressing push rod 43 enters rotor
In the through hole 421 of 42, the helical form of push rod 43 rotates protruding 431 helical forms acting on through hole 421 and rotates
Sliding limit 422 drive rotor 42 rotates about the axis thereof, and rotor 42 drives described cylindricality by rotating linkage structure
Rotational structure rotates accommodating in chamber 31, and in groove 413 and groove 413, the magnetic bead of receiving turns to towards the
In the region of two cavitys.By local, the side of cylindricality rotational structure 41 is set to groove 413, and make
Groove 413 absorption the previous dynasty to the first cavity 10, treat that magnetic bead is adsorbed onto this groove 413 under the effect of magnetic force
On, then rotate through receiving chamber by push rod 42 drive rotor and cylindricality rotational structure 41, at rotation process
Gap can be formed between further groove 413 and the curved surface cavity wall accommodating chamber, and be adsorbed on groove 413
Magnetic bead because the existence of this gap scrapes off without the cavity wall being received chamber comes off, thus realizes material effectively
Transhipment, other positions simultaneously removing groove 413 in cylindricality rotational structure 41 can be with the sealing of receiving chamber 31
Join, it is ensured that the mutual independence between the first cavity and the second cavity of the unclassified stores in addition to magnetic bead.
When implementing, in the embodiment of the present invention, cylindricality rotational structure 41 is set to the cylindricality that shaft section is H-shaped
Structure, it includes upper groove 411 and low groove 412 and by upper groove, low groove separate linking district 414.
Wherein, rotor 42 is connected linkage with cylindricality rotational structure 41 in upper groove 411.In this preferred embodiment,
Rotating linkage structure 424 is the square shaped teeth being arranged at rotor with cylindricality rotational structure, and square shaped teeth arranges rotor 42
Bottom, the region being connected mutually with the bottom of rotor with cylindricality rotational structure 41；It is positioned at bottom and the post of rotor
The square shaped teeth of shape rotational structure engages each other linking when push rod pressing advances and realizes linkage, outwards pulls out at push rod
It is separated from each other when going out or eject.It addition, magnet install and controlled be formed on reagent cartridge with the use of outside
Connecing on equipment, magnet is arranged on low groove or enters low groove 412 when magnetic bead absorption and transfer.At magnetic bead
Before transfer, groove 413 is towards the first cavity 10, and magnetic bead, under the magneticaction that magnet produces, concentrates on recessed
In groove 413；When magnetic bead shifts, pressing push rod 43 enters in the through hole 421 of rotor 42, push rod 43
Helical form rotates protruding 431 helical forms acting on through hole 421 and rotates sliding limit 422 drive rotor 42 around its axle
Line rotates, and rotor 42 drives described cylindricality rotational structure to rotate within accommodating chamber 31 by rotating linkage structure,
The magnetic bead accommodated in groove 413 and groove 413 turns in the region of the second cavity.For magnet
Select with material from the point of view of, when implementing, magnet is permanent magnet or electromagnet.At embodiments of the invention
Middle selection permanent magnet or extraordinary preferred version can be by the electromagnet that energising controls.
In a preferred embodiment of the invention, the first cavity 10 is arranged in parallel with the second cavity 20, therefore,
During magnetic bead transfer, push rod 43 drive rotor half revolution, correspondingly, cylindricality rotational structure 41 is passed through
Rotate linkage structure 424 under the drive of rotor 42, also rotate half cycle.Certainly other embodiment party of the present invention
In case, the first cavity 10 can not parallel be arranged with the second cavity 20, and the two exists certain angle,
And now, push rod 42 advances size and cylindricality rotational structure 41 angle also by the first cavity 10 and the second chamber
Angle between body 20 is determined, so that the magnetic bead in the first cavity 10 can turn accurately and efficiently
Move in the second cavity.
It addition, the reagent cartridge of the present invention also includes a upper cover 50.As shown in Figure 3,4, upper cover 50 is arranged
Peripheral in push rod 43 and suppress and mutually hold in the mouth in described cylindricality rotational structure and the first cavity 10 and the second cavity 20
The receiving cavity wall connect, upper cover 50 offers the guide through hole 431 positioning described push rod, and push rod 42 runs through
Guide through hole 431 extends to cylindricality rotational structure 41, thus plays the fixing and guiding to push rod 42 is good and make
With.
It addition, for be accurately defined rotor 42, the initial position of cylindricality rotational structure 41, it is ensured that groove can
In magnetic bead transfer previous dynasty to the first cavity 10, also for ensureing push rod pressing progradation rotor 42 and cylindricality
Rotational structure 41 selects half cycle exactly and is ultimately towards the second cavity 20, rotor 42 is provided with one spacing
Protruding 423, upper cover 50 is additionally provided with the card matching and limiting rotor rotation half cycle with spacing preiection 423
Position district (not shown).Spacing preiection 423 is parallel to the bottom land of groove, and screens district supports before rotor rotates
It is against the spacing preiection initial position with location rotor, thus limits at the beginning of rotor 42, cylindricality rotational structure 41
Beginning position, it is ensured that groove can shift the previous dynasty to the first cavity 10 at magnetic bead；Limit convex after rotor half revolution
Rise and be resisted against the screens district covering opposite side, thus ensure push rod pressing progradation rotor 42 and cylindricality
Rotational structure 41 selects half cycle exactly and is ultimately towards the second cavity 20.
It should be noted that from Fig. 1 and 2 structure it can be seen that the embodiment of the present invention provide setting
The reagent cartridge having pressing type magnetic bead transfer device only comprises the first cavity 10 and the second cavity 20 two is adjacent
The cavity arranged.It is true that using the reagent cartridge of only two cavitys as this in the preferred embodiment of the present invention
A kind of preferred implementation of scheme of the invention, is to start with from simple structure, illustrates that the present invention is nucleic acid
Or the one that during albumen preparation and detection, material transfer between adjacent step provides is efficiently, solve efficiently
Certainly scheme, but be not limited thereto, in actual applications, owing to relating to during nucleic acid extraction and detection
Material to multiple steps shifts, and also has multiple even if relating to the material transfer step by magnetic bead realizes,
Therefore, in other embodiments of the invention this reagent cartridge also can comprise the multiple above-mentioned side being mutually linked
Reagent cartridge described in case.The pressing type magnetic bead transfer device that i.e. embodiment of the present invention provides includes first,
Two, three ... ..n cavity, it is provided with pressing type magnetic bead transfer device between two adjacent cavitys.
The reagent cartridge being provided with pressing type magnetic bead transfer device that above-described embodiment discloses is by being disposed adjacent
Cavity between pressing type magnetic bead transfer device, the cylindricality rotational structure in this pressing type magnetic bead transfer device
Side be provided with accommodate magnetic bead groove, groove magnetic bead transfer the previous dynasty to described first cavity, magnet is by magnetic
Pearl is adsorbed in described groove；During magnetic bead transfer, push rod pressing advances rotor driven to rotate, and rotor is by rotation
Turning linkage structure drives the rotation of described cylindricality rotational structure that the magnetic bead in the first cavity is transferred to the second cavity
In, so that the magnetic bead of groove attachment turns in the second cavity, thus realize magnetic bead and be adsorbed in magnetic bead
Material to be extracted (protein, nucleic acid) fully, shift efficiently, thus overcomes nucleic acid, albumen etc.
The trivial operations that exists because linking operations such as material transfers between each step during the extraction of material, detection,
Sample or the problem of reagent vulnerable to pollution.It addition, the pressing type magnetic bead transfer device of the present invention, not only self
Simple in construction, and easy to operate, due to the ingehious design in pressing type magnetic bead transfer device, as long as along axle
To promoting push rod, can be by the propelled longitudinally rotation changing into rotor and cylindricality rotational structure of push rod, by recessed
The magnetic bead of groove attachment turns in the second cavity, and when push rod is outwards extracted or ejects, rotor turns with cylindricality
Linkage structure between dynamic structure lost efficacy, and rotor separates with cylindricality rotational structure under the drive of push rod and then returns
Lapse to position, be switched to quickly and easily next step or other be provided with the examination of pressing type magnetic bead transfer device
Agent cartridge respective operations.Therefore, it is not required to arrange in peripheral auxiliary equipment carry exclusively for magnetic bead transhipment
Rotating machinery for rotational power, it is only necessary to the power source of axial motive force is provided, thus effectively drops
The complexity of the equipment of low matched use；It addition, rotate sliding limit and phase therewith by arranging helical form
The helical form of coupling rotates projection, in conjunction with spacing preiection and screens district with the use of, not only compared to existing
Screw-driven greatly improve transmission efficiency, and effectively rotation radian is limited in half cycle, thus
Ensure that the groove of transfer magnetic bead can be accurately positioned and half revolution, thus realize magnetic bead and magnetic bead absorption material
Abundant transfer.
The above is the preferred embodiment of the present invention, it is noted that for the common skill of the art
For art personnel, under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications, this
A little improvements and modifications are also considered as protection scope of the present invention.
Claims (10)
1. being provided with a reagent cartridge for pressing type magnetic bead transfer device, this reagent cartridge includes being disposed adjacent
The first cavity and the second cavity and by interval region spaced apart to described first cavity and the second cavity；Its
It is characterised by: forming a receiving chamber in this interval region, described receiving intracavity is provided with one by the first cavity
The pressing type magnetic bead transfer device that is transferred in the second cavity of magnetic bead, described pressing type magnetic bead transfer device is close
Envelope is embedded in described receiving intracavity；
Described pressing type magnetic bead transfer device includes rotor and the push rod promoting described rotor to rotate, described rotor
Bottom is provided with the cylindricality rotational structure driven by rotor；Described rotor is provided with a through hole along its axis,
Being provided with helical form in this through hole and rotate sliding limit, the outer surface of this push rod is provided with and rotates sliding with described helical form
While the helical form matched rotates projection；
Described cylindricality rotational structure is arranged at described receiving intracavity and forms sealed spacer region by the with accommodating chamber
One cavity and the second cavity separate, and the joining place of described cylindricality rotational structure and rotor is provided with rotation linkage knot
Structure；When push rod pressing advances, rotor will by rotating linkage structure drive described cylindricality rotational structure rotation
Magnetic bead in first cavity is transferred in the second cavity；When push rod is outwards extracted or ejects, rotor and post
Linkage structure between shape rotational structure lost efficacy, rotor separate with cylindricality rotational structure under the drive of push rod and
Rear revolution playback；
The side of described cylindricality rotational structure is provided with the groove accommodating magnetic bead, also sets up in described interval region
There is the magnet of absorption magnetic bead；Described groove magnetic bead transfer the previous dynasty to described first cavity, described magnet is by magnetic
Pearl is adsorbed in described groove；During magnetic bead transfer, pressing push rod enters in the through hole of rotor, the spiral of push rod
Shape rotates the protruding helical form rotation sliding limit drive rotor acting on through hole and rotates about the axis thereof, and rotor is by rotation
Turning linkage structure drives described cylindricality rotational structure accommodating intracavity rotation, attachment in described groove and groove
Magnetic bead turns in the region of the second cavity.
Being provided with the reagent cartridge of pressing type magnetic bead transfer device the most as claimed in claim 1, its feature exists
In: described cylindricality rotational structure is set to the column construction that shaft section is H-shaped, and it includes upper groove and recessed
Groove and by upper groove, low groove separate linking district, described rotor rotates with cylindricality in described upper groove
Structure cohesion links.
Being provided with the reagent cartridge of pressing type magnetic bead transfer device the most as claimed in claim 2, its feature exists
In: described rotation linkage structure is the square shaped teeth being arranged at described rotor with cylindricality rotational structure, described square
Tooth arranges the bottom of described rotor, the region being connected mutually with the bottom of rotor with cylindricality rotational structure；It is positioned at institute
State the bottom of rotor and the square shaped teeth of cylindricality rotational structure when push rod pressing advances, engage each other linking realization connection
Dynamic, it is separated from each other when push rod is outwards extracted or ejects.
Being provided with the reagent cartridge of pressing type magnetic bead transfer device the most as claimed in claim 2, its feature exists
In: described magnet install and controlled be formed on reagent cartridge with the use of external equipment on, magnet is arranged on
Low groove or magnetic bead absorption and transfer time enter low groove.
Being provided with the reagent cartridge of pressing type magnetic bead transfer device the most as claimed in claim 1, its feature exists
In: described magnet is permanent magnet or electromagnet.
Being provided with the reagent cartridge of pressing type magnetic bead transfer device the most as claimed in claim 1, its feature exists
In: described first cavity and the second cavity be arranged in parallel, in magnetic bead transfer process, described push rod drive rotor
Half revolution, described cylindricality rotational structure also rotates half cycle by rotating linkage structure under the drive of rotor.
Being provided with the reagent cartridge of pressing type magnetic bead transfer device the most as claimed in claim 1, its feature exists
In: also include that a upper cover, described upper cover are arranged at pressing type magnetic bead transfer device suppressing and turn in described cylindricality
The receiving cavity wall that dynamic structure and the first cavity and the second cavity are mutually linked, described on cover and offer location
The guide through hole of described rotor, described push rod runs through described guide through hole and extends to rotor.
Being provided with the reagent cartridge of pressing type magnetic bead transfer device the most as claimed in claim 7, its feature exists
In: be provided with a spacing preiection on described rotor, described on cover be additionally provided with and described spacing preiection mutually
Join and limit rotor and rotate the screens district of half cycle.
Being provided with the reagent cartridge of pressing type magnetic bead transfer device the most as claimed in claim 8, its feature exists
In: described spacing preiection is parallel to the bottom land of described groove, and screens district is resisted against described limit before rotor rotates
Position is protruding to position the initial position of rotor, and after rotor half revolution, spacing preiection is resisted against and covers opposite side
Screens district to limit rotor and the equal half revolution of rotational parts.
10. the reagent cartridge being provided with pressing type magnetic bead transfer device, it is characterised in that: this reagent card
Box comprises the multiple reagent cartridges as described in any one in claim 1-9 being mutually linked.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610329938.7A CN105950438B (en) | 2016-05-17 | 2016-05-17 | A kind of reagent cartridge being provided with push type magnetic bead transfer device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610329938.7A CN105950438B (en) | 2016-05-17 | 2016-05-17 | A kind of reagent cartridge being provided with push type magnetic bead transfer device |
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| CN105950438A true CN105950438A (en) | 2016-09-21 |
| CN105950438B CN105950438B (en) | 2019-08-16 |
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| CN201610329938.7A Active CN105950438B (en) | 2016-05-17 | 2016-05-17 | A kind of reagent cartridge being provided with push type magnetic bead transfer device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111746913A (en) * | 2020-06-30 | 2020-10-09 | 盛振华 | Biological reagent detect reagent box |
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| US20150232832A1 (en) * | 2012-02-13 | 2015-08-20 | Neumodx Molecular, Inc. | System and method for processing and detecting nucleic acids |
| CN204608019U (en) * | 2015-04-09 | 2015-09-02 | 余家昌 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
| CN204607980U (en) * | 2015-04-10 | 2015-09-02 | 余家昌 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
| CN204832194U (en) * | 2015-07-03 | 2015-12-02 | 艾康生物技术(杭州)有限公司 | Biological sample testing cassette |
| CN205188260U (en) * | 2015-11-13 | 2016-04-27 | 余家昌 | Reagent card box of liquid level slope when material shifts |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150232832A1 (en) * | 2012-02-13 | 2015-08-20 | Neumodx Molecular, Inc. | System and method for processing and detecting nucleic acids |
| CN204608019U (en) * | 2015-04-09 | 2015-09-02 | 余家昌 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
| CN204607980U (en) * | 2015-04-10 | 2015-09-02 | 余家昌 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
| CN204832194U (en) * | 2015-07-03 | 2015-12-02 | 艾康生物技术(杭州)有限公司 | Biological sample testing cassette |
| CN205188260U (en) * | 2015-11-13 | 2016-04-27 | 余家昌 | Reagent card box of liquid level slope when material shifts |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111746913A (en) * | 2020-06-30 | 2020-10-09 | 盛振华 | Biological reagent detect reagent box |
| CN111746913B (en) * | 2020-06-30 | 2021-11-05 | 杭州快格科技有限公司 | Biological reagent detect reagent box |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105950438B (en) | 2019-08-16 |
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Effective date of registration: 20170526 Address after: 200120 Shanghai Pudong New Area city wide 222 Lane 6, 5 Dan Applicant after: Bio Technology (Shanghai) Co., Ltd. Address before: 361, Ruth Town, San Francisco, Calif. Applicant before: Yu Jiachang Applicant before: Ai Jiesi bio tech ltd Applicant before: Bio Technology (Shanghai) Co., Ltd. |
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