CN106148184A - A kind of reagent cartridge being provided with magnetic bead transfer organization - Google Patents
A kind of reagent cartridge being provided with magnetic bead transfer organization Download PDFInfo
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- CN106148184A CN106148184A CN201510165627.7A CN201510165627A CN106148184A CN 106148184 A CN106148184 A CN 106148184A CN 201510165627 A CN201510165627 A CN 201510165627A CN 106148184 A CN106148184 A CN 106148184A
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- magnetic bead
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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Abstract
A kind of reagent cartridge being provided with magnetic bead transfer organization includes the first cavity being disposed adjacent and the second cavity and by interval region spaced apart to described first cavity and the second cavity；A receiving chamber is formed in this interval region, described receiving intracavity is provided with a magnetic bead transfer organization being transferred in the second cavity by the magnetic bead in the first cavity, described magnetic bead transfer organization seals and is embedded in described receiving intracavity, described magnetic bead transfer organization includes cylindricality rotational structure and the screw mandrel promoting described cylindricality rotational structure to rotate, and described screw mandrel promotes cylindricality rotational structure to rotate the transfer realizing magnetic bead at receiving intracavity.The solution of the present invention, by arranging magnetic bead transfer organization between adjacent cavity, makes magnetic bead and is adsorbed in the material to be extracted of magnetic bead fully, shift efficiently；It addition, the reagent cartridge not only self structure of the present invention is simple, and easy to operate, it is possible to be effectively reduced the complexity of the equipment of matched use.
Description
Technical field
The present invention relates to preparation and the consumptive material used by detection of nucleic acid or protein, particularly relate to one and be provided with
The reagent cartridge of magnetic bead transfer organization.
Background technology
Along with transformation and the development of personalized medicine of medical model, medical test circle in the urgent need to quickly,
Accurate detection means, Molecular Detection has then given play to the advantage of uniqueness.The basis of Molecular Detection is that analysis is tested
The histiocyte of survey person, hair, anticoagulation or blood stasis mark, and formaldehyde is fixed, in paraffin-embedded tissue
Gene and expression product, by complete on molecular level nucleic acid (DNA and RNA) detect, in disease
Before once occurring symptom, sign and biochemical change the most not yet occur, just can accurately make detection.
At present, molecular detection technology mainly has making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biological core
Chip technology etc..Molecular Detection product is mainly used in the clinical departments such as tumor, infection, heredity, Prenatal Screening
Detection, and MEC, technical service center, third party testing agency and microbial rapid detection city
The aspects such as field.Currently, the measuring means such as blood routine, cytology, pathology and immunology is all towards automatically
Change, integration, standardization direction are developed, but due to himself technical complexity of Molecular Detection, " from sample to
Result " full-automatic instrument platform few or there is many insoluble technical problems.Such as, exist
The centrifugal column method or the paramagnetic particle method that use in biological technical field carry out nucleic acid extraction, it is generally required to carry out cracking,
In conjunction with four steps such as, rinsing, eluting, add follow-up making nucleic acid molecular hybridization, polymerase chain reaction (PCR)
With detecting steps such as biochips, the full-automatic instrument of whole " from sample to result " is made to be very difficult to reality
Existing, single for the transfer of effective ingredient in each step, the modes using manual transfer in prior art more,
I.e. by the way of artificial by raw material or intermediate product at step successively and the reaction vessel that realizes step successively
In shift, it is therefore apparent that in prior art use manual material branch mode, not only complex operation,
Waste time and energy.What is more also resides in whole operating process and easily causes pollution, affects the purity of extract,
And material is difficult to fully, shifts efficiently, affect experimental result.Therefore, in view of Molecular Detection its from
Body technical complexity, the full-automatic instrument platform of " from sample to result " is few or there is many being difficult to and solves
Technical problem certainly, will realize abundant, the transfer efficiently of material between each step and be accomplished by consumptive material and reality
That tests equipment structurally and functionally carries out many considering, and can avoid such as becoming present in prior art
The problems such as this height, poor sensitivity, structure complexity, operation and associated assay devices are excessively complicated.
Summary of the invention
For deficiency of the prior art, present invention solves the technical problem that and be to provide one to be provided with magnetic bead
The reagent cartridge of transfer organization, by arranging magnetic bead transfer organization between adjacent cavity, makes magnetic bead and suction
Invest the material to be extracted (protein, nucleic acid) of magnetic bead fully, shift efficiently.
Present invention solves the technical problem that and a kind of reagent cartridge being provided with magnetic bead transfer organization of offer is be provided,
Not only self structure is simple, and easy to operate, it is possible to be effectively reduced the answering of equipment of matched use
Polygamy.
In order to solve above-mentioned technical problem, the reagent being provided with magnetic bead transfer organization that the embodiment of the present invention provides
Cartridge includes the first cavity being disposed adjacent and the second cavity and described first cavity and the second cavity is spaced
The interval region opened；Forming a receiving chamber in this interval region, described receiving intracavity is provided with one by the first chamber
Internal magnetic bead is transferred to the magnetic bead transfer organization in the second cavity, and described magnetic bead transfer organization seals and is embedded in
Described receiving intracavity；
Described magnetic bead transfer organization includes cylindricality rotational structure and the silk promoting described cylindricality rotational structure to rotate
Bar, described cylindricality rotational structure is arranged at described receiving intracavity and forms sealed spacer region by the with accommodating chamber
One cavity and the second cavity separate, and described cylindricality rotational structure is provided with a screwed hole along its axis, described
Being provided with female thread in screwed hole, this female thread matches with the external screw thread of screw mandrel outer surface；
The side of described cylindricality rotational structure is gone back in being provided with the adsorbing plane accommodating magnetic bead, described interval region
It is provided with the magnet of absorption magnetic bead；Described adsorbing plane magnetic bead transfer the previous dynasty to described first cavity, described
Magnetic bead is adsorbed in described adsorbing plane by magnet；During magnetic bead transfer, axially promote silk along cylindricality rotational structure
Bar, screw mandrel enters in the screwed hole of cylindricality rotational structure, and the external screw thread of screw mandrel acts on the female thread of screwed hole
Cylindricality rotational structure is promoted to accommodate what intracavity rotation, described adsorbing plane and adsorbing plane adhered to around its axis
Magnetic bead turns in the region of the second cavity.
As the preferred version of the present invention, the one that embodiments of the invention provide is provided with magnetic bead transfer organization
Reagent cartridge farther include the part or all of of techniques below feature:
Preferably, described screw mandrel is provided with a through hole along wherein axis direction, and described magnet is when magnetic bead shifts
Interting and adsorb magnetic bead in described through hole, after completing magnetic bead transfer, described magnet exits described through hole.
Preferably, the both sides of described magnetic bead transfer organization are provided with two and are parallel to described cylindricality rotational structure axle
The magnet of line, described magnet produces toroidal magnetic field in opposite direction.
Preferably, described magnet is permanent magnet or electromagnet.
Preferably, the joining place of described first cavity and interval region is set to the contraction structure become narrow gradually,
At the cohesive position of the first cavity Yu cylindricality rotational structure, the first cavity is contracted to corresponding with described adsorbing plane
Width.
Preferably, the joining place of described second cavity and interval region is set to the contraction structure become narrow gradually,
At the cohesive position of the second cavity Yu cylindricality rotational structure, the second cavity is contracted to corresponding with described adsorbing plane
Width.
Preferably, described first cavity and the second cavity be arranged in parallel, in magnetic bead transfer process, and described cylindricality
Rotational structure rotates half cycle, and described screw mandrel is external screw thread or outer along the axial actuating length of cylindricality rotational structure
The half pitch of screw thread.
Preferably, described magnetic bead transfer organization also includes that a gland, described gland are arranged at described screw mandrel periphery
And suppress the receiving cavity wall being mutually linked in described cylindricality rotational structure and the first cavity and the second cavity, institute
Stating and offer the guide through hole positioning described screw mandrel on gland, described screw mandrel runs through described guide through hole and extends to
Described cylindricality rotational structure.
Preferably, the externally threaded end of described screw mandrel is provided with chamfering.
It addition, the reagent cartridge being provided with magnetic bead transfer organization that the embodiment of the present invention provides, this reagent cartridge
In comprise the reagent cartridge described in the multiple such schemes being mutually linked.What i.e. the embodiment of the present invention provided sets
The reagent cartridge being equipped with magnetic bead transfer organization includes first, second and third ... ..n cavity, two adjacent cavitys it
Between be provided with magnetic bead transfer organization.
The reagent cartridge being provided with magnetic bead transfer organization that the embodiment of the present invention provides, has the advantages that
The reagent cartridge being provided with magnetic bead transfer organization that the embodiment of the present invention provides is by the chamber being disposed adjacent
Magnetic bead transfer organization between body, the side of the cylindricality rotational structure in this magnetic bead transfer organization is provided with receiving magnetic
The adsorbing plane of pearl, adsorbing plane magnetic bead transfer the previous dynasty to described first cavity, magnetic bead is adsorbed to by magnet
In described adsorbing plane；During magnetic bead transfer, screw mandrel promotes cylindricality rotational structure accommodating intracavity rotation, makes suction
The magnetic bead of attached plane attachment turns in the second cavity, thus realizes magnetic bead and be adsorbed in the thing to be extracted of magnetic bead
Material (protein, nucleic acid) is fully, shift efficiently.Thus overcome carrying of the material such as nucleic acid, albumen
Take, detect during exist because linking operations such as material transfers between each step trivial operations, sample or
The problem of reagent vulnerable to pollution.It addition, the reagent cartridge being provided with magnetic bead transfer organization of the present invention, not only
Self structure is simple, and easy to operate, due to the cylindricality rotational structure in magnetic bead transfer organization and screw mandrel
Ingehious design, as long as screw mandrel is pushed axially, can change into cylindricality rotational structure by the propelled longitudinally of screw mandrel
Rotation, make the cylindricality rotational structure mated with wire rod thread rotate accommodating intracavity, adsorbing plane adhered to
Magnetic bead turn in the second cavity.Therefore, it is not required to arrange exclusively for magnetic in peripheral auxiliary equipment
Pearl transhipment provides the rotating machinery of rotational power, it is only necessary to provide the power source of axial motive force, thus
Significantly reduce the complexity of the equipment of matched use.
Accompanying drawing explanation
Fig. 1 is that the structure of the reagent cartridge being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides is shown
It is intended to one.
Fig. 2 is that the structure of the reagent cartridge being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides is shown
It is intended to two.
Fig. 3 is the screw mandrel in the reagent cartridge being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides
Structural representation.
Fig. 4 is the cylindricality in the reagent cartridge being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides
The structural representation of rotational structure.
Fig. 5 is the cavity in the reagent cartridge being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides
The structural representation of structure.
Detailed description of the invention
The embodiment of the present invention provides a kind of reagent cartridge being provided with magnetic bead transfer organization to include: be disposed adjacent
First cavity and the second cavity and by interval region spaced apart to described first cavity and the second cavity；Between Gai
Forming a receiving chamber in territory, septal area, described receiving intracavity is provided with 1 and the magnetic bead in first cavity is transferred to the
Magnetic bead transfer organization in two cavitys, described magnetic bead transfer organization seals and is embedded in described receiving intracavity；Described
Magnetic bead transfer organization includes cylindricality rotational structure and the screw mandrel promoting described cylindricality rotational structure to rotate, described post
Shape rotational structure is arranged at described receiving intracavity and forms sealed spacer region by the first cavity and the with accommodating chamber
Two cavitys separate, and described cylindricality rotational structure is provided with a screwed hole along its axis, sets in described screwed hole
Being equipped with female thread, this female thread matches with the external screw thread of screw mandrel outer surface；The one of described cylindricality rotational structure
Side is provided with the adsorbing plane accommodating magnetic bead, is additionally provided with the magnet of absorption magnetic bead in described interval region；Institute
State adsorbing plane magnetic bead transfer the previous dynasty to described first cavity, magnetic bead is adsorbed to described absorption by described magnet
In plane；During magnetic bead transfer, axially promoting screw mandrel along cylindricality rotational structure, screw mandrel enters cylindricality rotational structure
Screwed hole in, the external screw thread of screw mandrel act on screwed hole female thread promote cylindricality rotational structure around its axis
Rotating accommodating intracavity, the magnetic bead of described adsorbing plane and adsorbing plane attachment turns to towards the second cavity
In region.
Wherein, described screw mandrel is provided with a through hole along wherein axis direction, and described magnet was worn when magnetic bead shifts
Inserting absorption magnetic bead in described through hole, after completing magnetic bead transfer, described magnet exits described through hole.
Wherein, the both sides of described magnetic bead transfer organization are provided with two and are parallel to described cylindricality rotational structure axis
Magnet, described magnet produce toroidal magnetic field in opposite direction.
Wherein, described magnet is permanent magnet or electromagnet.
Wherein, the joining place of described first cavity and interval region is set to the contraction structure become narrow gradually,
First cavity and the cohesive position of cylindricality rotational structure, the first cavity is contracted to corresponding with described adsorbing plane
Width.
Wherein, the joining place of described second cavity and interval region is set to the contraction structure become narrow gradually,
Second cavity and the cohesive position of cylindricality rotational structure, the second cavity is contracted to corresponding with described adsorbing plane
Width.
Wherein, described first cavity and the second cavity be arranged in parallel, and in magnetic bead transfer process, described cylindricality turns
Dynamic structure rotates half cycle, and described screw mandrel is external screw thread or outer spiral shell along the axial actuating length of cylindricality rotational structure
The half pitch of stricture of vagina.
Wherein, described magnetic bead transfer organization also includes that a gland, described gland are arranged at described screw mandrel periphery also
Suppress the receiving cavity wall being mutually linked in described cylindricality rotational structure and the first cavity and the second cavity, described
Offering the guide through hole positioning described screw mandrel on gland, described screw mandrel runs through described guide through hole and extends to institute
State cylindricality rotational structure.
Wherein, the externally threaded end of described screw mandrel is provided with chamfering.
Wherein, the reagent cartridge being provided with magnetic bead transfer organization that the embodiment of the present invention provides, this reagent cartridge
In comprise the reagent cartridge described in the multiple such schemes being mutually linked.What i.e. the embodiment of the present invention provided sets
The reagent cartridge being equipped with magnetic bead transfer organization includes first, second and third ... ..n cavity, two adjacent cavitys it
Between be provided with magnetic bead transfer organization.
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clearly
Chu, be fully described by, it is clear that described embodiment be only a part of embodiment of the present invention rather than
Whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not making creation
The every other embodiment obtained under property work premise, broadly falls into the scope of protection of the invention.
The knot of the reagent cartridge being provided with magnetic bead transfer organization that Fig. 1 and 2 provides for the preferred embodiment of the present invention
Structure schematic diagram.This reagent cartridge being provided with magnetic bead transfer organization is applied to nucleic acid or albumen preparation and detection sets
In Bei, it is intended to during preparing for nucleic acid or albumen and detect, the material transfer between adjacent step provides one
Efficiently, solution efficiently, but be not limited thereto, in actual applications, described extraction box also can be answered
Extraction for other biological extract.
As Figure 1-5, the reagent cartridge bag being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides
Include the first cavity 10 and the second cavity 20 and by interval spaced apart to the first cavity 10 and the second cavity 20
Region 30.Structure from Fig. 1 and 2 shows, the first cavity 10 and the second cavity 20 are open-type knot
Structure, it practice, this is only a kind of implementation of the present invention, in other embodiments of the invention, the
One cavity 10 and the second cavity 20 also can be arranged as required to as in addition to interval region 30, other each
It is the cavity body structure of closing, and when the reagent cartridge being provided with magnetic bead transfer organization of the present invention is used in core
When the extraction of acid and test experience, in addition to interval region 30, other each faces are the cavity body structure of closing
It it is more commonly used structure.
When implementing, forming a receiving chamber 31 in this interval region 30, being provided with one in accommodating chamber 31 will
Magnetic bead in first cavity 10 is transferred to the magnetic bead transfer organization 40 in the second cavity, magnetic bead transfer organization 40
Sealing is embedded in receiving intracavity 31.Wherein, magnetic bead transfer organization 40 includes cylindricality rotational structure 41 and promotes
The screw mandrel 42 that cylindricality rotational structure 41 rotates.During implementing, cylindricality rotational structure 41 is arranged at appearance
Receive intracavity and with accommodate chamber formed sealed spacer region the first cavity 10 and the second cavity are separated 20, cylindricality turns
Dynamic structure 41 is provided with a screwed hole 411 along its axis, is provided with female thread 412 in screwed hole 411,
This female thread 412 matches with the external screw thread 421 of screw mandrel 42 outer surface.When screw mandrel is along cylindricality rotational structure
41 when being longitudinally propelling, and by screw-driven, screw mandrel 42 axial translation is converted into turning of cylindricality rotational structure 41
Dynamic, cylindricality rotational structure 41 is accommodating intracavity rotation.
For the ease of screwing, the externally threaded end of screw mandrel is provided with chamfering 4211.It addition, magnetic bead transfer knot
Structure 40 also includes a gland 43.As in figure 2 it is shown, gland 43 is arranged at described screw mandrel periphery and suppresses in institute
State cylindricality rotational structure and the first cavity 10 and receiving cavity wall that the second cavity 20 is mutually linked, gland 43
On offer the guide through hole 431 positioning described screw mandrel, screw mandrel 42 runs through guide through hole 431 and extends to cylindricality
Rotational structure 41, thus play the fixing and guide effect good to screw mandrel 42.
In order to shift magnetic bead fully, efficiently, the side of cylindricality rotational structure 41 is provided with receiving magnetic bead
Adsorbing plane 413, be additionally provided with in interval region 30 absorption magnetic bead magnet (not shown).From principle
On say, the present invention is mainly by using the magnetic-particle including magnetic bead as extracting nucleic acid, albumen
Or other macromole bio-extracts, magnetic bead the first cavity 10 realize to nucleic acids in samples, albumen or other
The adsorption of macromole bio-extract, in the first cavity 10, on the magnetic bead in mixed material, absorption needs to be carried
Nucleic acid, albumen or other macromole bio-extracts taken.Now, i.e. before magnetic bead transfer, adsorbing plane 413
Towards the first cavity 10, magnetic bead is adsorbed in adsorbing plane 413 by magnet；When magnetic bead shifts, along cylindricality
Rotational structure 41 axially promotes screw mandrel 42, and screw mandrel 42 enters in the screwed hole 411 of cylindricality rotational structure, silk
The external screw thread 421 of bar 42 acts on the female thread 412 of screwed hole and promotes cylindricality rotational structure holding around its axis
Intracavity of receiving rotates, and the magnetic bead accommodated in adsorbing plane 413 and adsorbing plane 413 turns to towards the second cavity
In the region of 10.By local, the side of cylindricality rotational structure 41 is set to adsorbing plane 413, and make
Adsorbing plane 413 absorption the previous dynasty to the first cavity 10, treat that magnetic bead is adsorbed onto this absorption under the effect of magnetic force
In plane 413, then promote cylindricality rotational structure 41 to rotate through receiving chamber by screw mandrel 42, rotating
Journey can form arcuate gap between adsorbing plane 413 and the curved surface cavity wall accommodating chamber, and be adsorbed on suction
Magnetic bead in attached plane 413 because the existence of this arcuate gap scrapes off without the cavity wall being received chamber comes off,
Thus realize material transfer effectively, simultaneously except other positions of adsorbing plane 413 in cylindricality rotational structure 41
Can mate with accommodating chamber 31 sealing, it is ensured that the unclassified stores in addition to magnetic bead is at the first cavity and the second cavity
Between mutual independence.
It addition, from the point of view of the structure of the first cavity 10 and the second cavity 20 with the joining place of interval region is arranged,
In order to shift magnetic bead fully, efficiently, the first cavity 10 is set to the joining place of interval region 30
The contraction structure become narrow gradually, at the cohesive position of the first cavity 10 with cylindricality rotational structure, the first cavity 10
It is contracted to the width corresponding with described adsorbing plane 413.Correspondingly, the second cavity 20 and interval region
Joining place be also configured as the contraction structure that becomes narrow gradually, in the second cavity 20 and cylindricality rotational structure 41
Cohesive position, the second cavity 20 is contracted to the width corresponding with described adsorbing plane 413.To this end, the first chamber
The joining place of body 10 and the second cavity 20 and interval region may be configured as chamfering and receives the structure on limit, the most also may be used
In mode as shown in Figure 5, the width of the first cavity 10 and the second cavity 20 is set near it
With the structure gradually narrowed during the joining place of interval region.
In a preferred embodiment of the invention, the first cavity 10 is arranged in parallel with the second cavity 20, therefore,
During magnetic bead transfer, screw mandrel 42 needs to promote cylindricality rotational structure 41 accommodating intracavity rotation half cycle, with
Accordingly, the axial translation of screw mandrel 42 advances this size of half pitch.Certainly in other realities of the present invention
Executing in scheme, the first cavity 10 can not parallel be arranged with the second cavity 20, and the two exists certain folder
Angle, and now, screw mandrel 42 advances size and cylindricality rotational structure 41 angle also by the first cavity 10 and
Angle between two cavitys 20 is determined, so that the magnetic bead in the first cavity 10 can accurately, efficiently
Be transferred in the second cavity.
As shown in figs. 1 and 3, in the preferred embodiments of the present invention 1, screw mandrel 42 sets along wherein axis direction
Being equipped with a through hole 422, magnet interts when magnetic bead shifts and adsorbs magnetic bead in through hole, completes magnetic after magnetic bead transfer
Body exits through hole 422.In this scheme, the working method of magnetic bead transfer organization 40 is: before magnetic bead shifts,
Adsorbing plane 413 is towards the first cavity 10, and magnet interts and adsorbs magnetic bead in through hole 422, is adsorbed by magnetic bead
To adsorbing plane 413；When magnetic bead shifts, axially promote screw mandrel 42, screw mandrel along cylindricality rotational structure 41
In 42 screwed holes 411 entering cylindricality rotational structure, the external screw thread 421 of screw mandrel 42 acts on the interior of screwed hole
Screw thread 412 promotes cylindricality rotational structure to rotate at receiving intracavity around its axis, and adsorbing plane 413 and absorption are flat
The magnetic bead accommodated in face 413 turns in the region of the second cavity 10；When adsorbing plane 413 and absorption
The magnetic bead accommodated in plane 413 turns to after the second cavity 10, and magnet exits in through hole 422, magnetic bead
Owing to losing the adsorption of magnet magnetic force, it is dispersed in the second cavity 20.
In embodiments of the invention 2, the both sides of magnetic bead transfer organization are provided with two and are parallel to cylindricality rotation
The magnet of structure axis, magnet produces toroidal magnetic field in opposite direction, so that magnetic bead moves to adsorbing plane 413.
In this scheme, the working method of magnetic bead transfer organization 40 is: before magnetic bead shifts, adsorbing plane 413 court
To the first cavity 10, magnetic bead, under the magneticaction that toroidal magnetic field is formed, concentrates in adsorbing plane 413,
And this magnetic direction is identical with the shift direction of magnetic bead；When magnetic bead shifts, along cylindricality rotational structure 41 axle
To promoting screw mandrel 42, screw mandrel 42 enters in the screwed hole 411 of cylindricality rotational structure, the external screw thread of screw mandrel 42
421 female threads 412 acting on screwed hole promote cylindricality rotational structure accommodating intracavity rotation around its axis, inhale
The magnetic bead accommodated in attached plane 413 and adsorbing plane 413 turns in the region of the second cavity 10.
From the point of view of selection and the material of magnet, when implementing, magnet is permanent magnet or electromagnet.?
Embodiments of the invention 1 select permanent magnet or can be extraordinary by the electromagnet that energising controls
Preferred version.And in the embodiment of the present invention two, use electromagnet can not only produce institute easily and efficiently
The toroidal magnetic field needed, and can be controlled accurately the action time in magnetic field (i.e. magnetic bead transfer time)
System, after magnetic bead is transferred to the second cavity, magnetic bead rapid dispersion from adsorbing plane enters in the second cavity
The reaction of one step.
It should be noted that from Fig. 1 and 2 structure it can be seen that the embodiment of the present invention provide setting
The reagent cartridge having magnetic bead transfer organization only comprises the first cavity 10 and the second cavity 20 two is disposed adjacent
Cavity.It is true that using the reagent cartridge of only two cavitys as side of the present invention in the preferred embodiment of the present invention
A kind of preferred implementation of case, is to start with from simple structure, illustrates that the present invention is nucleic acid or albumen
The one that during preparation and detection, material transfer between adjacent step provides efficiently, solution efficiently,
But it is not limited thereto, in actual applications, owing to relating to multiple step during nucleic acid extraction and detection
Rapid material transfer, also has multiple even if relating to the material transfer step by magnetic bead realizes, therefore,
Other embodiments of the present invention also can comprise described in the multiple such schemes being mutually linked in this reagent cartridge
Reagent cartridge.The reagent cartridge being provided with magnetic bead transfer organization that i.e. embodiment of the present invention provides includes first,
Two, three ... ..n cavity, it is provided with magnetic bead transfer organization between two adjacent cavitys.
The reagent cartridge being provided with magnetic bead transfer organization that above-described embodiment discloses is by the cavity being disposed adjacent
Between magnetic bead transfer organization, the side of the cylindricality rotational structure in this magnetic bead transfer organization is provided with receiving magnetic bead
Adsorbing plane, adsorbing plane magnetic bead transfer the previous dynasty to described first cavity, magnetic bead is adsorbed to institute by magnet
State in adsorbing plane；During magnetic bead transfer, screw mandrel promotes cylindricality rotational structure accommodating intracavity rotation, makes absorption
The magnetic bead of plane attachment turns in the second cavity, thus realizes magnetic bead and be adsorbed in the material to be extracted of magnetic bead
(protein, nucleic acid) fully, shift efficiently.Thus overcome the material such as nucleic acid, albumen extraction,
Trivial operations, sample or the reagent existed because of linking operations such as material transfers between each step during detection
The problem of vulnerable to pollution.It addition, the reagent cartridge being provided with magnetic bead transfer organization of the present invention, not only self
Simple in construction, and easy to operate, ingenious due to the cylindricality rotational structure in magnetic bead transfer organization and screw mandrel
Design, as long as screw mandrel is pushed axially, can change into turning of cylindricality rotational structure by the propelled longitudinally of screw mandrel
Dynamic, make the cylindricality rotational structure mated with wire rod thread accommodate intracavity rotation, the magnetic adhered to by adsorbing plane
Pearl turns in the second cavity.Therefore, it is not required to arrange in peripheral auxiliary equipment turn exclusively for magnetic bead
Fortune provides the rotating machinery of rotational power, it is only necessary to provide the power source of axial motive force, thus effectively
Reduce the complexity of equipment of matched use.
The above is the preferred embodiment of the present invention, it is noted that for the common skill of the art
For art personnel, under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications, this
A little improvements and modifications are also considered as protection scope of the present invention.
Claims (10)
1. being provided with a reagent cartridge for magnetic bead transfer organization, this reagent cartridge includes first be disposed adjacent
Cavity and the second cavity and by interval region spaced apart to described first cavity and the second cavity；Its feature exists
In: forming a receiving chamber in this interval region, described receiving intracavity is provided with one by the magnetic bead in the first cavity
The magnetic bead transfer organization being transferred in the second cavity, described magnetic bead transfer organization seals and is embedded in described receiving chamber
In；
Described magnetic bead transfer organization includes cylindricality rotational structure and the silk promoting described cylindricality rotational structure to rotate
Bar, described cylindricality rotational structure is arranged at described receiving intracavity and forms sealed spacer region by the with accommodating chamber
One cavity and the second cavity separate, and described cylindricality rotational structure is provided with a screwed hole along its axis, described
Being provided with female thread in screwed hole, this female thread matches with the external screw thread of screw mandrel outer surface；
The side of described cylindricality rotational structure is gone back in being provided with the adsorbing plane accommodating magnetic bead, described interval region
It is provided with the magnet of absorption magnetic bead；Described adsorbing plane magnetic bead transfer the previous dynasty to described first cavity, described
Magnetic bead is adsorbed in described adsorbing plane by magnet；During magnetic bead transfer, axially promote silk along cylindricality rotational structure
Bar, screw mandrel enters in the screwed hole of cylindricality rotational structure, and the external screw thread of screw mandrel acts on the female thread of screwed hole
Cylindricality rotational structure is promoted to accommodate what intracavity rotation, described adsorbing plane and adsorbing plane adhered to around its axis
Magnetic bead turns in the region of the second cavity.
It is provided with the reagent cartridge of magnetic bead transfer organization the most as claimed in claim 1, it is characterised in that: institute
Stating screw mandrel and wherein axis direction is provided with a through hole, described magnet interted into described through hole when magnetic bead shifts
Middle absorption magnetic bead, after completing magnetic bead transfer, described magnet exits described through hole.
It is provided with the reagent cartridge of magnetic bead transfer organization the most as claimed in claim 1, it is characterised in that: institute
The both sides stating magnetic bead transfer organization are provided with two magnets being parallel to described cylindricality rotational structure axis, described
Magnet produces toroidal magnetic field in opposite direction.
It is provided with the reagent cartridge of magnetic bead transfer organization the most as claimed in claim 1, it is characterised in that: institute
Stating magnet is permanent magnet or electromagnet.
It is provided with the reagent cartridge of magnetic bead transfer organization the most as claimed in claim 1, it is characterised in that: institute
State the contraction structure that the joining place of the first cavity and interval region is set to become narrow gradually, at the first cavity and post
The cohesive position of shape rotational structure, the first cavity is contracted to the width corresponding with described adsorbing plane.
It is provided with the reagent cartridge of magnetic bead transfer organization the most as claimed in claim 1, it is characterised in that: institute
State the contraction structure that the joining place of the second cavity and interval region is set to become narrow gradually, at the second cavity and post
The cohesive position of shape rotational structure, the second cavity is contracted to the width corresponding with described adsorbing plane.
It is provided with the reagent cartridge of magnetic bead transfer organization the most as claimed in claim 1, it is characterised in that: institute
Stating the first cavity and the second cavity be arranged in parallel, in magnetic bead transfer process, described cylindricality rotational structure rotates half
In week, described screw mandrel is external screw thread or externally threaded half pitch along the axial actuating length of cylindricality rotational structure.
It is provided with the reagent cartridge of magnetic bead transfer organization the most as claimed in claim 1, it is characterised in that: institute
State magnetic bead transfer organization and also include that a gland, described gland are arranged at described screw mandrel periphery and suppress in described post
The receiving cavity wall that shape rotational structure and the first cavity and the second cavity are mutually linked, described gland offers
Positioning the guide through hole of described screw mandrel, described screw mandrel runs through described guide through hole and extends to described cylindricality rotation knot
Structure.
It is provided with the reagent cartridge of magnetic bead transfer organization the most as claimed in claim 1, it is characterised in that: institute
The externally threaded end stating screw mandrel is provided with chamfering.
10. the reagent cartridge being provided with magnetic bead transfer organization, it is characterised in that: this reagent cartridge is wrapped
Containing the multiple reagent cartridges as described in any one in claim 1-9 being mutually linked.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510165627.7A CN106148184B (en) | 2015-04-09 | 2015-04-09 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
| PCT/CN2015/087220 WO2016161739A1 (en) | 2015-04-09 | 2015-08-17 | Reagent cartridge provided with magnetic bead transferring structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510165627.7A CN106148184B (en) | 2015-04-09 | 2015-04-09 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
Publications (2)
| Publication Number | Publication Date |
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| CN106148184A true CN106148184A (en) | 2016-11-23 |
| CN106148184B CN106148184B (en) | 2018-08-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510165627.7A Active CN106148184B (en) | 2015-04-09 | 2015-04-09 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
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| Country | Link |
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| CN (1) | CN106148184B (en) |
| WO (1) | WO2016161739A1 (en) |
Cited By (2)
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| WO2017080531A1 (en) * | 2015-11-13 | 2017-05-18 | 奥然生物科技（上海）有限公司 | Reagent cartridge which tilts liquid surface during material transfer |
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Also Published As
| Publication number | Publication date |
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| CN106148184B (en) | 2018-08-31 |
| WO2016161739A1 (en) | 2016-10-13 |
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Effective date of registration: 20170519 Address after: 200120 Shanghai Pudong New Area city wide 222 Lane 6, 5 Dan Applicant after: Bio Technology (Shanghai) Co., Ltd. Address before: 361, Ruth Town, San Francisco, Calif. Applicant before: Yu Jiachang Applicant before: Ai Jiesi bio tech ltd Applicant before: Bio Technology (Shanghai) Co., Ltd. |
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