WO2021068911A1

WO2021068911A1 - Rotating disc type multiple measurement device and system

Info

Publication number: WO2021068911A1
Application number: PCT/CN2020/120085
Authority: WO
Inventors: 王东; 李泉
Original assignee: 深圳华迈兴微医疗科技有限公司
Priority date: 2019-10-11
Filing date: 2020-10-10
Publication date: 2021-04-15
Also published as: CN110646608A

Abstract

Provided are a rotating disc type multiple measurement device and a system. The multiple detection device comprises: at least one magnetic particle luminescence micro-fluidic chip (1), the magnetic particle luminescence micro-fluidic chip (1) comprising a chip body (11), and sample adding parts (111) being arranged on the chip body (11); a rotating disc (2), the rotating disc (2) being provided with a sample adding pool (21) for adding samples and a plurality of sample conveying channels (22) communicated with the sample adding pool (21) respectively, each sample conveying channel (22) being arranged independently from one another, and each sample conveying channel (22) being connected with the corresponding sample adding part (111) respectively, at least one end of the sample conveying channel (22) being provided with a blocking cover. When the rotating disc (2) rotates, samples in the sample adding pool (21) on the rotating disc (2) can be uniformly separated into each sample conveying channel (22) due to the centrifugal force, and flow to the sample adding part (111) from the sample conveying channel (22), thus a large number of samples to be measured can be added into the sample adding pool (21) all at once, then the samples simultaneously flow to each magnetic particle luminescence micro-fluidic chip (1) respectively without one-by-one manual operation, thereby greatly improving measurement efficiency.

Description

Rotary disc type multi-joint inspection device and system

Technical field

The invention belongs to the technical field of microfluidic chip luminescence immunodetection, and in particular relates to a rotary disc type multi-joint inspection device and system.

Background technique

At present, in vitro diagnostics (IVD) has two main development trends: one is automation and integration, that is, the use of fully automated, highly sensitive large-scale instruments and equipment in the central laboratory supporting large hospitals to achieve high-precision disease analysis and diagnosis. ; Another kind of miniaturization, bedside, that is, through the palm of the small simple device, to achieve rapid on-site analysis and diagnosis. However, small hospitals have insufficient funds and small sample sizes, making them unsuitable for purchasing expensive large-scale equipment. Therefore, the rapid detection equipment used by most hospitals at this stage is mainly test strips and its supporting equipment, but the test strips can only achieve qualitative or semi-quantitative detection, with low detection sensitivity, poor specificity, poor repeatability, and obvious interference. Due to the large population of China, the increasing ageing, and the sharp increase in the incidence rate, relying solely on large hospitals has become overwhelmed. Therefore, it is extremely urgent to develop rapid detection methods and equipment with simple operation, high sensitivity, good repeatability and quantitative accuracy.

Microfluidic chip technology integrates basic operation units such as sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes onto a micron-scale chip to automatically complete the entire analysis process. Because of its great potential in the fields of biology, chemistry, medicine, etc., it has developed into a multidisciplinary research field of biology, chemistry, medicine, fluids, materials, machinery, etc., and has been applied to biomedical research, biochemical testing, forensic appraisal, etc. field. The existing microfluidic chip needs to perform an operation before performing the luminescence immunoassay, that is, adding the sample to the microfluidic chip. However, the existing sample adding method only passes the samples into each microfluidic chip one by one manually or mechanically. When the number of samples is large and multiple microfluidic chips are needed to realize joint inspection at the same time, if the samples are still passed into the corresponding microfluidic chips one by one, and then a pair of microfluidic chips are tested separately. It is said that the detection efficiency is low, and it is impossible to achieve the purpose of simultaneous large-scale sample addition and detection.

Summary of the invention

The embodiment of the present invention provides a rotating disc-type multi-joint inspection device and system, which aims to solve the problem that the existing samples need to be added to the microfluidic chip one by one, and the microfluidic chip is tested separately, resulting in low efficiency.

The embodiment of the present invention is implemented in this way, providing a turntable type multi-joint inspection device, the multi-joint inspection device includes: at least one magnetic particle light-emitting microfluidic chip, the magnetic particle light-emitting microfluidic chip includes a chip body, so The chip body is provided with a sample addition part; a turntable, on which a sample addition pool for sample addition and a plurality of sample injection channels respectively connected with the sample injection pool are provided, and each sample injection channel is set independently of each other, and Each sample infusion channel is respectively connected to the corresponding sample adding part, and at least one end of the sample infusion channel is provided with a blocking cover.

Furthermore, the multi-joint inspection device further includes: a connection area interconnected with the turntable, and the connection area is provided with connection channels respectively communicating with the sample addition part and the sample delivery channel. .

Furthermore, the turntable and the connecting area are integrally arranged.

Furthermore, a buffer pool is provided on the connection area, and the width of the buffer pool is greater than the width of the connection channel.

Further, the first side of the connection area extends toward the second side and is gradually reduced in width, and the first side is disposed adjacent to the chip main body.

Furthermore, the connecting channel is provided with a sharp part at one end close to the sample application part.

Further, the turntable is set to be circular, the sample addition pool is set to be a circle with a smaller diameter than the turntable, and the sample transfer channel is provided on the outer wall of the turntable and the sample addition pool. Between the outer walls.

The present invention also provides a turntable type multi-joint inspection system, which includes: the above-mentioned multiple joint inspection device; a driving device for driving the turntable to rotate; and under the drive of the driving device, the The sample application pool of the turntable separates the sample into each sample delivery channel, and the sample enters the sample application part from the sample delivery channel.

Furthermore, the driving device is a rotating electric machine.

The beneficial effect achieved by the present invention is to provide a turntable-type multi-joint inspection device and system, which is provided with a turntable and at least one magnetic particle luminescent microfluidic chip connected to the turntable. The sample transfer channel on the turntable and the magnetic particle luminescent microfluidic chip are provided. The sample application parts on the fluid control chip are connected to each other. When the turntable rotates, the sample in the sample injection pool on the turntable will be evenly separated into each sample injection channel due to centrifugal force, and flow from the sample injection channel to the sample injection part, so that it can be done once Add more samples to the sample pool for testing, and then flow the samples to each magnetic particle light-emitting microfluidic chip at the same time, without manual operation one by one, each magnetic particle light-emitting microfluidic chip is also set on the turntable together , Can achieve the purpose of joint inspection of multiple magnetic particle light-emitting microfluidic chips, greatly improving the detection efficiency, and the sample delivery channel is equipped with a block cover, when the magnetic particle light-emitting microfluidic chip is not connected to the corresponding position of the sample channel , Blocking the cover can close the sample transfer channel to avoid the loss of samples.

Description of the drawings

Figure 1 is a schematic diagram of a multi-joint inspection device provided by an embodiment of the present invention;

Figure 2 is a schematic diagram of a turntable provided by an embodiment of the present invention;

Fig. 3 is a schematic diagram of a magnetic particle light-emitting microfluidic chip provided by an embodiment of the present invention.

Detailed ways

In order to make the objectives, technical solutions, and advantages of the present invention clearer, the following further describes the present invention in detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, but not to limit the present invention.

Compared with the prior art, the present invention is a turntable type multi-joint inspection device and system, which is provided with a turntable 2 and at least one magnetic particle light-emitting microfluidic chip 1 connected to the turntable 2, and a sample transfer channel 22 on the turntable 2 and The sample application parts 111 on the magnetic particle luminescence microfluidic chip 1 are connected to each other. When the turntable 2 rotates, the sample in the sample application pool 21 on the turntable 2 will be uniformly separated into each sample delivery channel 22 due to centrifugal force, and will be separated from the sample delivery channel 22 by centrifugal force. The sample channel 22 flows to the sample adding part 111, so that more samples for testing can be added to the sample adding pool 21 at one time, and then the samples can be flowed to each magnetic particle light emitting microfluidic chip 1 at the same time, without manual operation one by one. Each magnetic particle light-emitting microfluidic chip is also jointly set on the turntable 2, which can achieve the purpose of joint inspection of multiple magnetic particle light-emitting microfluidic chips, greatly improving the detection efficiency, and the sample delivery channel 22 is provided with a blocking cover. When the magnetic particle light-emitting microfluidic chip 1 is not connected to the corresponding position of the sample infusion channel 22, the cap can close the sample channel 22 to avoid the loss of samples.

Example one

Referring to Figures 1 to 3, the first embodiment provides a rotary disc-type multi-joint inspection device, and the multi-joint inspection device includes:

At least one magneto-particle light-emitting microfluidic chip 1. The magneto-particle light-emitting microfluidic chip 1 includes a chip body 11 on which a sample addition part 111 is provided; a turntable 2, on which a sample addition pool for sample addition is provided 21 and a plurality of sample infusion channels 22 respectively connected to the sample addition cell 21, each sample infusion channel 22 is set independently of each other, and each sample infusion channel 22 is respectively connected to the corresponding sample addition part 111, at least one end of the sample infusion channel 22 With blocking cover.

In order to prevent the sample from contacting the external environment, an open or closed lid can be provided on the sample adding pool 21 of the turntable 2. After the lid is opened, the external sample adding device (such as a needle tube) can be passed through the through hole formed after the lid is opened. , The sample adding device adds the sample to the sample adding pool 21, and then closes the lid, which can isolate the sample from the external environment. Further, the size of the lid can be set to be much smaller than the size of the sample addition pool 21. As a result, the size of the through hole formed after the lid is opened is also smaller, which can better prevent impurities from the external environment from entering the lid and the sample addition pool 21. In the cavity between, the sample adding device adds the sample to the sample adding pool 21 through the through hole. Of course, the sample addition pool 21 can also be directly exposed to the external environment, and the sample addition device directly adds the sample into the sample addition pool 21.

After the sample adding device adds the sample to the sample adding pool 21, it rotates the turntable 2 to control the turntable 2 to move at a uniform speed, accelerate or decelerate, so that the turntable 2 realizes circular rotation. Due to the centrifugal force, the samples in the sample addition pool 21 will be evenly separated and enter each sample infusion channel 22. Since the sample infusion channels 22 are arranged independently of each other, the phenomenon of sample crossing can be avoided. As the turntable 2 continues to rotate, since the sample infusion channel 22 has a setting direction, it can guide the sample, and the sample will sequentially enter the corresponding sample adding part 111 from the sample infusion channel 22 to enter the chip body 11 smoothly. Optionally, the diameters of the sample delivery channels 22 are consistent with each other, which can ensure that the samples flowing from the sample delivery channel 22 into the sample application part 111 are consistent with each other.

It is worth mentioning that the magnetic particle light-emitting microfluidic chip 1 and the turntable 2 are detachable, and when the operator removes the sample part 111 from the sample delivery channel 22, the magnetic particle light-emitting microfluidic chip 1 and the turntable 2 are disassembled. At this time, the cap can close the sample infusion channel 22 to prevent subsequent samples from flowing out. When the operator connects the sample adding part 111 to the sample transfer channel 22, the combination of the magnetic particle light-emitting microfluidic chip 1 and the turntable 2 can be realized.

For the blocking cap, it can be set at one end of the sample infusion channel 22 close to the sample addition pool 21. When the sample infusion channel 22 is not connected to the sample addition part 111, the operator can close the blocking cap by himself, so that the blocking cap closes the sampling channel 22. Therefore, even if the turntable 2 rotates later, the sample will not enter the sample infusion channel 22 from the sample addition tank 21, which can avoid the waste of samples. When the sample infusion channel 22 is correspondingly connected to the sample adding section 111, the operator then opens the blocking cover by himself, and then the turntable 2 rotates, and the sample can enter the sample infusion channel 22 from the sample adding cell 21 and enter the sample adding section 111. Alternatively, the blocking cover can be set at one end of the sample infusion channel 22 close to the sample adding part 111. When the sample infusion channel 22 is not connected to the sample adding part 111, the operator can close the blocking cover by himself, and the operator can open the blocking cover by himself, even if it is subsequently When the turntable 2 rotates, the sample will only flow into the sample infusion channel 22, but will not flow out of the sample infusion channel 22, which can avoid the waste of the sample, and can help the sample to be separated in the sample addition tank 21 more uniformly. When the sample infusion channel 22 is correspondingly connected to the sample adding section 111, the operator then opens the blocking cover by himself, and then the turntable 2 rotates, and the sample can enter the sample infusion channel 22 from the sample adding cell 21 and enter the sample adding section 111.

Example two

1 and 3, on the basis of the first embodiment, the multi-joint inspection device of the second embodiment further includes:

The connection area 22 interconnected with the turntable is provided with connection channels 221 communicating with the sample adding portion 111 and the sample transfer channel 22 respectively.

Among them, after the turntable 2 rotates, the sample in the sample addition pool 21 is passed into each sample input channel 22, and then enters each corresponding connection channel 221 from the sample input channel 22, and finally enters each corresponding sample addition channel from the connection channel 221 In the part 111, the magnetic particle light-emitting microfluidic chip 1 is thus smoothly entered. It should be noted that the sample delivery channel 22 of the turntable 2 is in communication with the connection channel 221 of the connection area 22, and the connection channel 221 is then communicated with the sample addition portion 111 of the chip body, and the non-sample delivery channel 22 is directly connected to the addition channel 221. The sample parts 111 are connected to each other, so that the sample separation process is more reliable.

Example three

1, on the basis of the second embodiment, the connecting area 22 and the turntable 2 of the third embodiment are integrally arranged, which reduces the processing cost and greatly improves the overall firmness.

Example four

1 and 3, on the basis of the second embodiment, the connection area 22 of the fourth embodiment is provided with a buffer pool 222, and the width of the buffer pool 222 is greater than the width of the connection channel 221.

Wherein, the buffer pool 222 can optionally be set in the middle position of the connection channel 221. When the sample enters the connecting channel 221 from the sample adding pool 21, the sample moves along the guiding action of the connecting channel 221, and as the sample moves, the sample enters the buffer pool 222 to obtain a certain buffer effect, and then from the buffer The pool 222 flows out and continues to move along the guiding action of the connecting channel 221.

Example five

1 and 3, on the basis of the second embodiment, the first side of the connection area 22 of the fifth embodiment extends toward the second side and is gradually reduced in width, and the first side is disposed adjacent to the chip main body.

If the connection area 22 is provided with only the connection channel 221, the connection channel 221 is a long strip, which is relatively slender. As the turntable 2 rotates, the connection channel 221 also rotates with the turntable 2, and the connection channel 221 is in When rotating, it will receive a relatively large force, which will easily cause the connection channel 221 to bend, affect the transportation of the sample, and greatly reduce the service life of the connection channel 221. Therefore, the connection area 22 extends from the first side toward the second side and is arranged with a gradually reduced width, which can greatly improve the stability of the device and greatly improve the reliability of the connection between the turntable 2 and the magnetic particle light-emitting microfluidic chip 1. The turntable 2 rotates at a high speed, and the magnetic particle light-emitting microfluidic chip 1 may also be able to rotate stably as the turntable 2 rotates.

Wherein, the first side of the connection area 22 extends toward the second side and the width is gradually reduced to form a triangle shape. When the turntable 2 and the magnetic particle light-emitting microfluidic chip 1 are connected to each other, the second side of the connection area 22 can be connected to the The sample delivery channels 22 conflict with each other, or, the second side of the connection area 22 is far away from the sample delivery channel 22, and only the connection channel 221 and the sample delivery channel 22 are connected to each other.

Example Six

1 and 3, on the basis of the second embodiment, the connecting channel 221 of the sixth embodiment is provided with a sharp portion at one end close to the sample application portion 111, and the sharp end of the sharp portion faces the magnetic particle light-emitting microfluidic control Chip 1. Since the sample application part 111 on the chip body 11 is a through slot, when the magnetic particle light-emitting microfluidic chip 1 moves toward the turntable 2, the sharp part can be inserted into the sample application part 111 to realize the sample application part 111 and the connecting channel 221 interconnection. When the magnetic particle light-emitting microfluidic chip 1 is separated from the turntable 2, the sample application part 111 and the sharp part are separated from each other. It should be noted that the sharp part may be provided with a through hole to allow the sample to flow from the connection channel 221 to the sample application part 111, or the sharp part can be set to a size that can be inserted into the sample application part 111 and part of the gap can be left. The sample can then flow into the sample application part 111 from the gap.

Example Seven

1 and 2, on the basis of the first embodiment, the turntable 2 of the seventh embodiment is set to be circular, the sample pool 21 is set to be a circle with a smaller diameter than the turntable 2, and the sample transfer channel 22 is set in Between the outer wall of the turntable 2 and the outer wall of the sample adding tank 21. Since the sample infusion channel 22 is a channel extending along the sample addition pool 21, the sample infusion channel 22 is arranged between the outer wall of the turntable 2 and the outer wall of the sample addition pool 21, which can greatly improve the stability and reliability of the turntable 2. The quality of use of the sample delivery channel 22 can also be greatly improved. When the turntable 2 rotates at a high speed, the sample delivery channel 22 can also rotate stably without bending.

Example eight

The ninth embodiment provides a turntable-type multiple joint inspection system. The multiple joint inspection system includes: the multiple joint inspection devices as in the first to seventh embodiments; and a driving device for driving the turntable 2 to rotate.

Driven by the driving device, the turntable 2 continuously rotates, and the sample adding pool 21 of the turntable 2 separates the sample into each sample transfer channel 22, and the sample enters the sample adding part 111 from the sample transfer channel 22.

Example 9

On the basis of the eighth embodiment, the driving device of the ninth embodiment is a rotating electric machine. Rotating electric machines can convert electrical energy into mechanical energy, and mainly include an electromagnet winding or distributed stator windings to generate a magnetic field and a rotating armature or rotor. Under the action of the rotating magnetic field of the stator winding, current flows through the aluminum frame and is rotated by the action of the magnetic field. Of course, the driving device may also be other, as long as it can drive the turntable 2 to rotate, which will not be repeated here.

The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modification, equivalent replacement and improvement made within the spirit and principle of the present invention shall be included in the protection scope of the present invention. Inside.

Claims

A rotary disc type multi-joint inspection device, characterized in that the multi-joint inspection device comprises:

At least one magnetic particle light-emitting microfluidic chip, the magnetic particle light-emitting microfluidic chip comprising a chip body on which a sample adding part is provided;

The turntable is provided with a sample addition pool for adding samples and a plurality of sample infusion channels respectively connected with the sample addition pool, and each sample infusion channel is set independently of each other, and each sample infusion channel is connected to the corresponding sample injection channel. The parts are connected to each other, and at least one end of the sample delivery channel is provided with a blocking cover.
The multi-joint inspection device according to claim 1, wherein the multi-joint inspection device further comprises:

The connection area connected to the turntable is provided with connection channels respectively communicating with the sample adding part and the sample transfer channel.
The multi-joint inspection device according to claim 2, wherein the turntable and the connection area are integrally arranged.
The multi-joint inspection device according to claim 2, wherein a buffer pool is provided on the connection area, and the width of the buffer pool is greater than the width of the connection channel.
3. The multi-joint inspection device according to claim 2, wherein the first side of the connection area extends toward the second side and is gradually reduced in width, and the first side is disposed adjacent to the chip main body.
The multi-unit inspection device according to claim 2, wherein the connecting channel is provided with a sharp part at one end close to the sample adding part.
The multi-unit inspection device according to claim 1, wherein the turntable is set in a circular shape, the sample addition pool is set in a circular shape with a smaller diameter than the turntable, and the sample transfer channel is set in the Between the outer wall of the turntable and the outer wall of the sample adding pool.
A rotary disc type multi-joint inspection system, characterized in that, the multi-joint inspection system includes:

The multi-joint inspection device according to any one of claims 1 to 7;

A driving device for driving the turntable to rotate;

Driven by the driving device, the sample application pool of the turntable separates the sample into each sample delivery channel, and the sample enters the sample application part from the sample delivery channel.
The multi-joint inspection system according to claim 8, wherein the driving device is a rotating electric machine.