TWM534751U - Nucleic acid extractor - Google Patents

Description

Nucleic acid extraction device

The present invention relates to a nucleic acid extraction device, in particular to a mixing module and a stirring module and a magnetic module for mixing reagents and magnetic beads, and then using a multi-axis mobile platform to achieve an automated process. Nucleic acid extraction device.

According to the current, there are a variety of automated nucleic acid extractors on the market, which can be divided into four categories according to the principle of the method (Table 1). The description is as follows:

The first type is the basic application of the in-situ reaction. After the magnet attracts the magnetic beads at the bottom of the tank, the lysate, the cleaning solution and the eluent are replaced by a discharge gun to achieve the purpose of nucleic acid purification. The representative label model is MagNA Pure 96 (Roche). The disadvantage is that when the sample is thick or the product concentration is too high, it is easy to block the nozzle, causing abnormal suction and discharge functions, affecting the whole process, and if the liquid is dripping during the movement of the gun, there is cross-contamination of the sample. Possibility, while in the process of in situ reaction, false If the liquid is not completely removed, buffers or other impurities tend to remain in the product, forming a distracting factor in the back-end analysis, which is a major problem for future sophisticated molecular diagnostics.

Class II, as disclosed in US Pat. No. 6,509,193, B1, although the same as the first is the use of the suction and discharge system, the magnet is located on the side of the gun head, the magnetic beads can be fixed on the gun head, so the magnetic beads can be transferred to different slots, which are ex-in situ. The reaction thus eliminates the problem of the first type of in situ reaction acting as a buffer residue, but it is still impossible to avoid head clogging and liquid dripping.

Class III, as disclosed in US Pat. No. 6,448,092, B1, is also an ex situ reaction. The magnetic beads are transferred in different reagents by using a stirring rod sleeve and a magnetic rod. The stirring rod sleeve can be used as a reagent mixing and magnetic rod protection function when the magnetic beads are transferred. This method has no plugging problem of the first type of pipette tip, and there is no liquid transfer drip. At present, most automated nucleic acid extraction platforms adopt this method. However, the disadvantage of this method is that the sample is mixed by the stirring rod sleeve up and down, so the container needs to reserve more space to achieve better mixing efficiency.

The fourth type is a function of disturbing the reagent and the magnetic beads to achieve mixing by rotating the rod. The rotating rod can be magnetized by the battery excitation coil to magnetically and transfer the magnetic beads, and rotates compared with the original stirring action. The method can make full use of the container space and increase the sample usage. Taking the 96 deep hole plate as an example, the maximum safe operation volume is 1ml, and the rotation volume can be increased to 1.5. Above ml, increasing the sample size by up to 50% is of great help for the extraction of low concentration nucleic acid samples. The disadvantage of this device is that the excitation coil requires a considerable space, which limits the overall design and construction of the instrument, and is relatively expensive. In addition, the powerful electromagnet has a phase for the user equipped with the heart rate adjuster. When dangerous.

In the field of hand-held magnetic bead transfer devices, at present, only the lance gun and the magnetic seat are more convenient to use. However, the rifle mixing the sample, the magnetic beads or the buffer solution by suction and discharge still has the following disadvantages:

1. The gun head with the gun is too small. There may be a problem of blockage when mixing or extracting thicker liquid. It is easy to cause the liquid to backflush into the inside of the gun head, which risks cross-contamination.

2. The design of the pumping and discharging line needs to repeat the suction and discharge several times in order to make the liquid evenly mixed, time-consuming and laborious, and the efficiency is not good.

3. The gun discharges the liquid through the tip of the gun, which will produce shear force for the product with high viscosity and destroy the integrity of the product, which is unfavorable for the back-end inspection analysis.

4. When disposing the buffer solution, firstly, the fixed magnetic beads should be sucked on the bottom or side of the container with a magnetic seat, and then the liquid is removed by using a lance, and a new buffer is added. Due to the more or less residual water in the container and the personal factors during the operation of the gun, the old buffer can not be completely eliminated, and the cause of the back-end analysis is increased. At present, it can only be cleaned through multiple cleanings. Magnetic beads.

5. On the handheld device, it is difficult to operate multiple sets of samples at the same time (the common common size is 8 sets). All the steps including mixing and separating the magnetic beads must be performed separately by one sample. According to the above introduction, the automated or handheld nucleic acid extractors currently available on the market have their own advantages and disadvantages, and there is still a need for research and development.

In view of the deficiencies of the prior art, one of the purposes of this creation is to provide an automatic The nucleic acid extraction device uses a lifting module with a stirring module and a magnetic module to mix reagents and magnetic beads, and has a high degree of freedom during operation, and can be freely adjusted during the reaction process as needed. Reagents are designed to allow users to design their own extraction environment for a wide range of samples.

Another object of the present invention is to provide a nucleic acid extraction apparatus that can include a multi-axis mobile platform controlled by a computer to fully automate the nucleic acid extraction process.

Therefore, the present invention provides a nucleic acid extraction device comprising: a stirring module having a first driver, a gear set, a plurality of rotating shafts and a plurality of stirring rod sleeves, wherein the first driver is coupled to the gear set to Driving the gear set, each of the gear sets has a shaft hole in the center thereof, the plurality of rotating shafts are a hollow body and corresponding to the shaft holes of the plurality of gear sets and connected to the plurality of stirring rod sleeves; and a magnetic mold The group has a second driver, a lifting mechanism and a plurality of magnetic bars, one end of the plurality of magnetic bars being connected to the lifting mechanism, wherein the gear unit is driven by the first driver to be rotatably sleeved on the rotating shaft The rod sleeve is stirred for stirring; and the lifting mechanism drives the lifting mechanism to move the plurality of magnetic rods up and down, so that the plurality of magnetic rods enter and exit the plurality of stirring rod sleeves for magnetic attraction.

Preferably, the plurality of magnetic bars are driven by the second driving device to drive the plurality of magnetic bars to move downward to pass through the hollow body of the rotating shaft, and then correspondingly disposed in the plurality of stirring rod sleeves To magnetically.

Preferably, the plurality of magnetic bars are driven by the second actuator to drive the plurality of magnetic bars to move upward to disengage the plurality of stirring rod sleeves and release the magnetic attraction.

Preferably, the number of the plurality of magnetic bars and the number of the plurality of stirring bars are the same.

Preferably, the agitation module further includes a housing, the gear set being disposed within the housing.

Preferably, the gear set has a first gear set, a second gear and a third gear, the first gear set has a plurality of gears arranged in a row, and each tooth of the first gear set is mutually Engaging, the second gear is coupled to the first drive, and the third gear is disposed between the first gear set and the second gear.

Preferably, the stirrer sleeve has a stirring blade on the outer sleeve to increase the stirring efficiency. In a preferred embodiment, the agitating blades are at least two pieces and are disposed at a symmetrical position on the sleeve outside the agitating rod sleeve.

Preferably, the lifting mechanism has a coupling, a screw and a fixed platform, one end of the coupling is connected to the second driver, and the other end of the coupling is connected to the screw, and the screw is Connected to the fixed platform, the fixed platform is connected to the plurality of magnetic bars.

Preferably, the first driver is a brushless motor and the second driver is a stepping motor.

The creative nucleic acid extraction device further comprises a stripping device connected to the stirring rod sleeve, and the stripping device comprises a plurality of spring flaps, a plurality of guide rods and a stripping plate

Preferably, the spring blocking piece is used for the fixing platform to abut, and each guiding rod is correspondingly connected to each spring blocking piece, and the discharging plate is connected to the plurality of guiding rods. And a housing, and a return button is arranged on the housing for the user to manually press to remove the stirring rod sleeve.

The artificial nucleic acid extraction device further includes a multi-axis moving platform including an X-axis moving portion and a Y-axis moving portion.

Preferably, the X-axis moving part and the Y-axis moving part are mechanically connected to the lifting mechanism, and the X-axis moving part, the Y-axis moving part and the lifting mechanism are horizontally, vertically or by a computer. move up and down.

Preferably, the lifting mechanism extends a connecting portion and is connected to the Y-axis moving portion, and the rear end portion of the lifting mechanism is coupled to the X-axis moving portion to form an X-Y-Z three-axis moving platform.

10‧‧‧Agitator module

11‧‧‧First drive

12‧‧‧ Gear Set

121‧‧‧First gear set

1211‧‧‧ Gears

122‧‧‧second gear

123‧‧‧third gear

13‧‧‧ shaft

14‧‧‧ stir stick set

141‧‧‧Agitating blades

15‧‧‧Shell

20‧‧‧Magnetic Module

21‧‧‧Second drive

22‧‧‧ Lifting mechanism

221‧‧‧Coupling

222‧‧‧ screw

223‧‧‧Fixed platform

224‧‧‧Connecting Department

225‧‧‧ back end

23‧‧‧Magnetic rod

30‧‧‧Returning device

31‧‧‧Spring flaps

32‧‧‧guides

33‧‧‧Returning board

40‧‧‧Reaction tank for the first orifice plate

50‧‧‧Reaction tank for the second orifice plate

60‧‧‧X-axis moving part

70‧‧‧Y-axis moving part

The first figure shows a schematic view of the present nucleic acid extraction device.

The second figure is a schematic diagram of the first driver and gear set of the present nucleic acid extraction device.

The third figure is a front view of the present nucleic acid extraction device.

The fourth figure is a schematic diagram of the operation of one embodiment of the present invention. .

Next, the implementation of this creation is described in detail by the following examples, but is not limited thereto. The above and other objects, features and advantages of the present invention will become more apparent from

Please refer to the first figure for a schematic view of the nucleic acid extraction device of the present invention. The nucleic acid extraction device of the present invention comprises: a stirring module 10 having a first driver 11. A gear set 12, a plurality of rotating shafts 13 and a plurality of stirring rod sleeves 14. The first driving unit 11 is coupled to the gear set 12 to drive the gear set 12, and each of the gear sets 12 has a shaft hole therein ( The plurality of rotating shafts 13 are hollow bodies corresponding to the shaft holes of the plurality of gear sets 12 and connected to the plurality of stirring rod sleeves 14; and a magnetic module 20 having a second The driver 21, a lifting mechanism 22 and a plurality of magnetic bars 23 are connected to the lifting mechanism 22 at one end of the plurality of magnetic bars 23. The second driver 21 is configured to drive the lifting mechanism 22 to vertically move up and down, and simultaneously drive the plurality of magnetic bars 23 to move up and down; wherein the lifting mechanism 22 is driven by the second driver 21 to move the plurality of magnetic bars 23 upward. The plurality of magnetic bars 23 are separated from the plurality of stirring rod sleeves 14 to cancel the magnetic attraction; and the gear unit 12 is further driven by the first driver 11 to rotate the stirring rod sleeve 14 sleeved on the rotating shaft 13 for stirring. (rotating agitation), and driving the lifting mechanism 22 to drive the plurality of magnetic bars 23 to move downward by the second driving device 21, so that the plurality of magnetic bars 23 pass through the hollow bodies of the plurality of rotating shafts 13 to enter the plurality of stirring Rod sleeve 14 for magnetic attraction.

Please refer to the second figure and refer to the first figure, which shows a schematic diagram of the first driver and gear set of the present nucleic acid extraction device. The agitating module 10 further includes a housing 15 that is disposed within the housing 15. In the present example, the gear set 12 has a first gear set 121, a second gear 122, and a third gear. 123. The first gear set 121 includes a plurality of gears 1211 and is arranged in a row. Each gear 1211 of the first gear set 121 meshes with each other, and the second gear 122 is connected to the first driver 11. The third gear 123 is disposed between the first gear set 121 and the second gear 122 and at least one of the first gear set 121 The first gear 11 and the second gear 122 are meshed with each other. When the first gear 11 drives the second gear 122, the third gear 123 is driven by mutual engagement to generate a transmission, and the power is continuously generated to connect the first gear set 121. The rotating shaft 13 is driven to drive the stirring rod sleeve 14 to perform agitation, and the first driving unit 11 is a brushless motor, and the stirring rod sleeve 14 has a stirring blade 141 on the outer sleeve to increase the stirring efficiency.

The gear set 12 can be designed according to the needs of the user, and different numbers and sizes of gears can be disposed as needed, as long as the rotation of the stirring rod sleeve 14 by the gear set can be achieved, and is not particularly limited. Preferably, the first gear set 121 can include 8, 16, 32 or 96 gears, and the number of gears 1211 in the first gear set 121 corresponds to the number of the magnetic bars 23 and the stir bar sleeve 14 There is a one-to-one correspondence. For example, when the number of gears 1211 of the first gear set 121 has eight, the magnetic bar 23 and the agitating rod cover 14 also have eight at the same time to correspond to each other.

Please refer to the first figure, wherein the lifting mechanism 22 of the magnetic module 20 has a coupling 221, a screw 222 and a fixed platform 223, and one end of the coupling 221 is connected with the second driver 21, and the joint The other end of the shaft 221 is connected to the screw 222, and the screw 222 is connected to the fixed platform 223. The fixed platform 223 is connected to the plurality of magnetic bars 23, so that the second driver 21 drives the lifting mechanism. At 2 o'clock, the coupling 221, the screw 222 and the fixed platform 223 are driven to drive the plurality of magnetic bars 23 to move up and down. In the embodiment, the second driver 21 is a stepping motor.

In this embodiment, when the artificial nucleic acid extraction device performs rotational stirring, the stirring module 10 is moved to a reaction tank 40 of a first orifice plate (please refer to the fourth diagram). Wherein, a plurality of stirring rod sleeves 14 are respectively placed in the reaction tank 40 of the first orifice plate (please refer to the fourth drawing), and each of the reaction tanks 40 of the first orifice tray has a reaction solution (Fig. The second drive 21 drives the coupling 221 of the lifting mechanism 22, the screw 222, and the fixed platform 223 to move upward to drive the plurality of magnetic bars 23 out of the corresponding stirring rod sleeve 14, And driving the second gear 122 to rotate by the first driver 11, and driving the third gear 123, the third gear 123 driving the first gear set 121 to rotate, so as to drive the plurality of rotating shafts 13, so that the plurality of rotating shafts 13 The stirring rod sleeve 14 is rotated and stirred in the reaction tank 40 of the first orifice plate to be uniformly mixed. The rotation direction of the plurality of stirring rod sleeves 14 of the present invention can control the rotation direction of the gear set 12 by controlling the rotation direction of the second actuator 21 to achieve clockwise or counterclockwise rotation stirring.

In the embodiment, when the artificial nucleic acid extraction device performs magnetic attraction, the coupling 221, the screw 222, and the fixed platform 223 of the lifting mechanism 22 are driven to move downward by the second driver 21 to drive the A plurality of magnetic rods 23 enter the corresponding stirring rod sleeve 14, and the magnetic force of the magnetic rod 23 adsorbs the magnetic beads in the reaction tank 40 of the corresponding first orifice plate, so that the first orifice plate is in the reaction tank 40. The magnetic beads are adsorbed on the outer sleeve of the stirring rod sleeve 14. At this time, the user can manually move the stirring module 10 and the magnetic module 20 to the reaction tank 50 of the other second orifice plate (please refer to the fourth And the second drive 21 is used to drive the coupling 221 of the lifting mechanism 22, the screw 222 and the fixed platform 223 to move upwards to drive the plurality of magnetic bars 23 out of the stirring rod sleeve 14 again to end the magnetic field. Soaking, the magnetic beads adsorbed on the sleeve outside the plurality of stirring rod sleeves 14 fall into the reaction tank 50 of the second orifice tray, and then, again The first gear 11 drives the second gear 122 to rotate, and drives the third gear 123. The third gear 123 drives the first gear set 121 to rotate to drive the plurality of rotating shafts 13 to make the plurality of stirrings. The rod sleeve 14 is rotated and stirred in the reaction tank 50 of the second orifice plate to mix the magnetic beads and the reaction solution to be uniformly mixed.

Please refer to the third figure and with reference to the first figure, which shows a front view of the present nucleic acid extraction device. The nucleic acid extraction device further includes a stripping device 30, the stripping device 30 is connected to the stirring rod sleeve 14, and the stripping device 30 can include a plurality of spring flaps 31, a plurality of guide rods 32, and a retreat a material plate 33, wherein the spring blocking piece 31 is used for the fixing platform 223 to abut, and each guiding rod 32 is correspondingly connected to each spring blocking piece 31, and the stripping plate 33 is connected to the plurality of guides The rod 32 and the housing 15 are provided with a return button (not shown) on the housing 15 for manual pressing operation by the user to remove the stirring rod sleeve 14 and, in addition, the stripping plate 33 may also be part of the housing 15, for example, a detachable bottom plate in the housing 15.

In this embodiment, the stirring rod sleeve 14 is to be detached from the rotating shaft 13, and can be manually or automatically operated. If the returning button provided on the casing 15 is pressed for manual operation, the return button is The stripping plate 33 is pushed to remove the stirring rod sleeve 14 from the rotating shaft 13; and if it is automatically operated, the second driving unit 21 is controlled by a computer to drive the coupling 221 and the screw of the lifting mechanism 22. The 222 and the fixed platform 223 are moved downwards. After the fixed platform 223 is lowered to a certain height, the plurality of guiding rods 32 and the plurality of spring blocking pieces 31 are pressed, and the stripping plate 33 is pressed downward to make the stirring. The rod sleeve 14 is detached from the rotating shaft 13.

Please refer to the fourth figure for showing another embodiment of the present nucleic acid extraction device. Make a schematic diagram. The artificial nucleic acid extraction device can be connected to an external power source and connected with the computer, and the computer controls the operation and recording of each component, that is, the artificial nucleic acid extraction device adopts full automatic operation, as shown in the figure, the nucleic acid extraction device is created as shown in the figure. Further comprising a multi-axis moving platform, the multi-axis moving platform includes an X-axis moving portion 60 and a Y-axis moving portion 70. In the embodiment, the X-axis moving portion 60 and the Y-axis moving portion 70 are mechanically The lifting mechanism 22 is connected to enable the artificial nucleic acid extraction device to drive and control the X-axis moving portion 60, the Y-axis moving portion 70 and the lifting mechanism 22 to move freely, thereby achieving automatic control. The mechanism 22 is extended to connect the connecting portion 224 to the Y-axis moving portion 70, and the rear end portion 225 of the lifting mechanism 22 is coupled to the X-axis moving portion 60 to form an XYZ three-axis moving platform to enable the present nucleic acid. The extraction unit can be moved horizontally, vertically or up and down.

In the present embodiment, when the magnetic nucleic acid extraction device is controlled by a computer to mix the magnetic beads with the reaction solution for a plurality of orifice disks, in the present embodiment, the Y-axis moving portion 70 is first controlled by a computer to move the artificial nucleic acid extraction device to The reaction tray 40 of the first orifice plate is further moved upward by the computer control lifting mechanism 22, the magnetic rod 23 is taken out of the stirring rod sleeve 14, and the first actuator 11 is driven to drive the gear set 12 to rotate the stirring rod sleeve 14 for stirring. After stirring, the computer controls the lifting mechanism 22 to move the magnetic rod 23 downward into the stirring rod sleeve 14 for magnetic attraction, and then controls the X-axis moving portion 60 by the computer to move the artificial nucleic acid extraction device to the second. At the reaction tank 50 of the orifice plate, and again, the computer control lifting mechanism 22 moves upward, and the magnetic rod 23 is taken out of the stirring rod sleeve 14, so that the magnetic beads adsorbed on the sleeve outside the plurality of stirring rod sleeves 14 fall into the first In the reaction tank 50 of the two-hole disc, the first driver 11 is driven to drive the gear set 12 to rotate the stirring rod sleeve 14 Stirring is performed to achieve full automation of the present nucleic acid extraction device.

Accordingly, the nucleic acid extraction device (hereinafter referred to as the present embodiment) of the foregoing embodiment has the following features, but is not limited thereto:

1. This embodiment uses a single-size stirring rod sleeve, with the adjustment of the rotation speed and the design of the forward and reverse rotation, which can be used regardless of the viscosity of the sample, without clogging, liquid backlash and the like.

2. In this embodiment, the stirring rod sleeve is used to rotate the liquid, and the positive and negative design can be used to uniformly mix the liquid spoiler, which is more efficient than the gun mixing, saves time and labor, and the rotation speed of the embodiment is The operational process can be programmed to reduce human error and increase the consistency and reproducibility of the experiment.

3. In this embodiment, the stirring is performed by a stirring rod sleeve, and no shear force is generated, and the product integrity is better.

4. The design of the magnetic bead transfer in this embodiment directly utilizes the built-in magnetic rod magnetic magnetic beads, and then transfers to the new buffer solution, which solves the problem that the old buffer solution cannot be eliminated, increases the purity of the product, and also compares the number of intermediate cleaning times. Less, reduce the amount of product loss.

5. This embodiment adopts the functions of stylized flow, rotary mixing and magnetic bar separation of magnetic beads, so that it is easy to operate multiple sets of samples (for example, conventional 8 sets of samples) at one time, and the operation time is greatly reduced.

In summary, those skilled in the art can understand various changes that can be made according to the implementation of the present invention without departing from the spirit of the present invention. Therefore, it is obvious that the implementations listed are not intended to limit the present invention, but are intended to cover modifications made in the spirit and scope of the present invention, as defined by the scope of the appended claims.

11‧‧‧First drive

13‧‧‧ shaft

14‧‧‧ stir stick set

15‧‧‧Shell

20‧‧‧Magnetic Module

21‧‧‧Second drive

22‧‧‧ Lifting mechanism

221‧‧‧Coupling

222‧‧‧ screw

223‧‧‧Fixed platform

Claims (15)

A nucleic acid extraction device includes: a stirring module having a first driver, a gear set, a plurality of rotating shafts and a plurality of stirring rod sleeves, the first driver being coupled to the gear set to drive the gear set, Each of the gear sets has a shaft hole, the plurality of shafts being a hollow body and corresponding to the shaft holes of the plurality of gear sets and connected to the plurality of stirring rod sleeves; and a magnetic module having a second a driving device, a lifting mechanism and a plurality of magnetic bars, wherein one end of the plurality of magnetic bars is connected to the lifting mechanism; wherein the gear unit is driven by the first driver to rotate the stirring rod sleeve sleeved on the rotating shaft to perform Stirring; and driving the lifting mechanism to drive the plurality of magnetic bars up and down by the second driving device, so that the plurality of magnetic bars enter and exit the plurality of stirring rod sleeves for magnetic attraction. The nucleic acid extraction device of claim 1, wherein the plurality of magnetic rods drive the lifting mechanism to drive the plurality of magnetic rods to move downward to pass through the hollow body of the rotating shaft It is placed in the plurality of stirring rod sleeves for magnetic attraction. The nucleic acid extraction device of claim 2, wherein the plurality of magnetic bars are driven by the second actuator to drive the plurality of magnetic bars to move upward to disengage the plurality of stirring rod sleeves and release the magnetic Suck. The nucleic acid extraction device of claim 1, wherein the agitation module further comprises a housing, the gear set being disposed within the housing. The nucleic acid extraction device of claim 1, wherein the gear set has a first gear set, a second gear, and a third gear, the first gear set having a plurality of gears arranged in a row, and Each of the teeth of the first gear set meshes with each other, and the second gear is coupled to the first drive, the third gear is disposed between the first gear set and the second gear. The nucleic acid extraction device of claim 1, wherein the first gear set has a plurality of gears. The nucleic acid extraction device according to claim 1, wherein the agitating rod has a stirring blade on the sleeve to increase the stirring efficiency. The nucleic acid extraction device of claim 1, wherein the agitating blades are at least two pieces and are disposed at a symmetrical position on the sleeve outside the agitating rod sleeve. The nucleic acid extraction device of claim 1, wherein the lifting mechanism has a coupling, a screw and a fixed platform, one end of the coupling is connected to the second driver, and the coupling is another One end is connected to the screw, and the screw is connected to the fixed platform, and the fixed platform is connected to the plurality of magnetic bars. The nucleic acid extraction device of claim 1, wherein the first actuator is a brushless motor and the second actuator is a stepping motor. The nucleic acid extraction device of claim 1, further comprising a returning device connected to the stirring rod sleeve, and the discharging device comprises a plurality of spring blocking pieces, a plurality of guiding rods and a Stripping board. The nucleic acid extraction device of claim 11, wherein the spring block The film is used for the fixed platform to be abutted, and each of the guiding rods is correspondingly connected to each of the spring blocking pieces, and the stripping plate is connected to the plurality of guiding rods and the shell, and a retreat is provided on the shell The material key is used for the user to manually press the operation to remove the stirring rod sleeve. The nucleic acid extraction device of claim 1, further comprising a multi-axis moving platform comprising an X-axis moving portion and a Y-axis moving portion. The nucleic acid extraction device according to claim 13, wherein the X-axis moving portion and the Y-axis moving portion are mechanically connected to the lifting mechanism, and the X-axis moving portion is driven and controlled by a computer. The shaft moving portion and the lifting mechanism move horizontally, vertically, or vertically. The nucleic acid extraction device of claim 14, wherein the lifting mechanism extends a connecting portion and is connected to the Y-axis moving portion, and the rear end portion of the lifting mechanism is coupled to the X-axis moving portion to form a XYZ three-axis mobile platform.