CN211645265U - Portable biochemical reactor - Google Patents

Abstract

A portable biochemical reactor is suitable for driving at least one stirring sleeve to move relative to at least one reagent box along a first axial direction, and comprises a rack unit and a variable-speed lifting unit. The rack unit is adapted to hold the at least one reagent cartridge. The variable speed lifting unit comprises a linear moving module, a variable speed transmission module and a rotating module. The linear movement module has a slider adapted to mount the at least one stirring sleeve, the slider is movable relative to the rack unit between a top position in which the slider is adapted to be remote from the at least one reagent cartridge and a bottom position in which the slider is adapted to be adjacent to the at least one reagent cartridge. The variable speed transmission module is provided with a transmission part and a guide part. The rotating module is used for driving the transmission part to rotate, further drives the sliding block to follow the guide rail to move along the first axial direction, and synchronously drives the at least one stirring sleeve to generate variable-speed linear movement.

Description

Portable biochemical reactor

Technical Field

The utility model relates to a biochemical reactor, in particular to a portable biochemical reactor.

Background

In a conventional biochemical reactor for Nucleic acid extraction (Nucleic acid extraction) using silica Magnetic Beads (Magnetic Beads), Nucleic acids (DNA or RNA) in a biological sample are extracted and used for biological detection such as Polymerase Chain Reaction (PCR).

When the biochemical reaction is performed, in order to increase the extraction rate of nucleic acid, the biological sample and the magnetic beads are repeatedly and fully mixed with reagents with different functions in a stirring manner, and the biochemical reaction procedures such as cracking, cleaning, nucleic acid recovery and the like are sequentially performed, so that the purified nucleic acid is finally extracted.

The stirring method is generally a method in which a motor is used in combination with a screw to stir the reagent in a linear motion at a constant speed. In order to achieve better stirring uniformity, a controller with higher price is often used and is matched with complex electronic control programming to achieve the stirring purpose. In addition, the motor needs to rotate for several circles to drive the screw to reach a certain moving stroke, so that the conventional biochemical reactor has higher power consumption. The motor and the screw rod are matched, so that the whole equipment has certain weight, is difficult to lighten and is not convenient to carry.

Such disadvantages make it difficult to use the existing biochemical reactors in first-line situations outside the laboratory, such as the quarantine site of the breeding industry or the forensic site …. And because the cost of the whole structure is higher, the popularization is difficult, and the space to be improved is extremely large in use.

Disclosure of Invention

An object of the utility model is to provide a portable biochemical reactor which can improve the stirring efficiency and reduce the cost and is favorable for carrying.

The utility model discloses a portable biochemical reactor is applicable to at least one stirring cover of drive and follows the relative at least reagent casket displacement of primary shaft, portable biochemical reactor contains the frame unit, and an at least variable speed lift unit.

The rack unit is adapted to hold the at least one reagent cartridge.

The at least one variable-speed lifting unit comprises a linear moving module, a variable-speed transmission module and a rotating module, the linear moving module is provided with a guide rail connected with the rack unit and a sliding block which is movably connected with the guide rail along the first axial direction and is suitable for installing the at least one stirring sleeve, the sliding block moves between a top end position and a bottom end position relative to the rack unit, the sliding block is suitable for being far away from the at least one reagent box in the top end position, the sliding block is suitable for being close to the at least one reagent box in the bottom end position, the variable-speed transmission module is provided with a transmission part and a guide part, the transmission part is provided with a pivot part and a guide part which are arranged in a reverse direction, the guide part is movably connected with the guide part, and one of the guide part and the pivot part of the transmission part is connected with the sliding block, the rotating module is connected to the other one of the guide part and the pivoting part of the transmission part and is used for driving the transmission part to rotate, so that the transmission part drives the sliding block to move along the guide rail along the first axial direction through the guide part in the rotating process, and synchronously drives the at least one stirring sleeve to generate linear movement with variable speed.

The utility model discloses a portable biochemical reactor, at least one reagent casket is filled with reagent to define the liquid level, the slider can also for the frame unit removes to being located top position reaches midpoint position between the bottom position, in during the midpoint position, the slider is applicable to the drive the bottom of an at least stirring cover is neighbouring the liquid level, just the slider removes to the instantaneous speed of midpoint position is slower than removing to neighbouring instantaneous speed during the top position.

The utility model discloses a portable biochemical reactor, the definition orthogonal in first axial and mutual orthogonal's second axial and third axial each other, the rotation module is followed the third axial with the guide is located the double-phase contralateral of guide rail to have power spare and output shaft, the power spare is connected the frame unit, the output shaft is followed the second axial pivot is located power spare, and for the linear movement module is connected the guide reaches the pivot portion of driving medium wherein another.

The utility model discloses a portable biochemical reactor, the slider is connected the guide, the pivot portion of driving medium is fixed the rotation module.

The utility model discloses a portable biochemical reactor, the guide part of driving medium is rectangular hole, the guide slide in the guide part.

The utility model discloses a portable biochemical reactor still contains reagent casket and moves and carry the unit to the definition quadrature in first axial second axial, reagent casket moves and carries the unit and include two edges the second axial set up in frame element move carry the track, can set up with moving in move and carry orbital the seat, and be used for the drive move the seat relative the drive module that frame element removed.

The utility model discloses a portable biochemical reactor, the drive module have install in rack, the pivot of carrying the seat is located the gear of frame unit, and be used for the drive the driving piece of gear.

The utility model discloses a portable biochemical reactor, the frame unit includes chassis and side bearer, the side bearer set up in the chassis to the opening has, the reagent casket moves the seat that moves that carries the unit and has the setting and be in move orbital pedestal portion, and can connect with tearing down the tool portion of pedestal portion, tool portion is used for installing an at least reagent casket, and can certainly the opening of side bearer is taken out and is put into.

The utility model discloses a portable biochemical reactor, reagent casket moves and carries unit still includes the heating module, it has seat portion to move the seat of carrying, and tool portion to move, seat portion sets up move and carry the track, and supply the heating module is installed, tool portion can connect with tearing open seat portion, and is used for installing an at least reagent casket, and have and correspond the through-hole of heating module.

The utility model discloses a portable biochemical reactor still contains the magnetic bead and shifts the unit to contain two variable speed lift units, be applicable to in the at least reagent casket and load the several magnetic bead, one of them variable speed lift unit's slider connect in the stirring cover, wherein another variable speed lift unit connects the magnetic bead shifts the unit, the magnetic bead shifts the unit and includes that at least one is inserted and locates the bar magnet in the at least stirring cover, at least one bar magnet has neighbouring the magnetism portion of the bottom of stirring cover works as the slider is located during the bottom position, magnetism portion be applicable to with the magnetic bead magnetism adsorbs in on the stirring cover.

The beneficial effects of the utility model reside in that: the variable-speed transmission module can drive the at least one stirring rod to generate variable-speed linear movement, so that the stirring efficiency is improved, the cost is reduced, the size and the weight are reduced, and the purpose of convenience in carrying is achieved.

Drawings

FIG. 1 is an exploded perspective view illustrating the position relationship of one embodiment of the portable biochemical reactor of the present invention with a stirring row unit and four reagent cartridges;

FIG. 2 is a perspective view illustrating the embodiment;

FIG. 3 is a schematic diagram illustrating this embodiment;

FIG. 4 is a fragmentary, partial cross-sectional view illustrating the positional relationship between four mixing sleeves and the reagent cartridge with a slide in a top position according to this embodiment;

FIG. 5 is a fragmentary, partial cross-sectional view similar to FIG. 4 illustrating the positional relationship between the mixing sheath and the reagent cartridge with the slide at a midpoint position;

FIG. 6 is a fragmentary, partial cross-sectional view similar to FIG. 4 illustrating the positional relationship between the mixing sheath and the reagent cartridge with the slide in a bottom position;

FIG. 7 is a fragmentary side elevation illustrating a variation of the embodiment;

FIG. 8 is a fragmentary, partially exploded perspective view illustrating the positioning of the stir bar in this embodiment in relation to a bar fixture unit;

FIG. 9 is a fragmentary, partial cross-sectional view similar to FIG. 6, illustrating the four magnetic rods in positional relationship with the mixing sleeve with the other slide in a bottom position.

Detailed Description

The present invention will be described in detail with reference to the accompanying drawings and examples.

Referring to fig. 1, 2, and 3, an embodiment of the portable biochemical reactor of the present invention defines a first axial direction L1, a second axial direction L2, and a third axial direction L3 orthogonal to each other, and is adapted to drive a stirring bar 22 to displace relative to four reagent cartridges 21 along the first axial direction L1.

The reagent cassettes 21 are arranged at intervals along the third axial direction L3. Each of the reagent cartridges 21 includes a plurality of reagent wells 211 arranged along the second axial direction L2 and filled with reagents 213, and a plurality of magnetic beads 212 accommodated in one of the reagent wells 211. The reagent 213 in each reagent well 211 defines a liquid level 214.

The stirring row member 22 has four stirring sleeves 221 spaced along the third axial direction L3. Each stirring sleeve 221 can be inserted into the corresponding reagent tank 211 and is immersed in the reagent 213 away from the liquid surface 214 or through the liquid surface 214 along the first axial direction L1.

The number of the reagent cassette 21 and the stirring sleeve 221 is not limited to four, and may be three or less or five or more. In other variations of the present embodiment, the reagent cartridge 21 may be replaced by a deep-well plate.

The embodiment comprises a rack unit 3, a reagent cassette transfer unit 4, two variable speed lifting units 5, a row fixing unit 6, a magnetic bead transfer unit 7, and a sterilizing unit 8.

The rack unit 3 is adapted to hold the reagent cartridge 21 and includes a bottom frame 31, two side frames 32, and two extension brackets 33.

The side frames 32 are disposed at intervals along the third axial direction L3 on the bottom frame 31, and each side frame 32 has an opening 321. In other variations of the present embodiment, only one of the side frames 32 may have the opening 321, but not limited thereto.

The extension brackets 33 are connected between the side frames 32 along the third axial direction L3 and are spaced apart along the second axial direction L2.

In the present embodiment, the side frames 32 are disposed on two opposite sides of the bottom frame 31 along the third axial direction L3. However, in other variations of this embodiment, the rack unit 3 may only include one side frame 32, and the extension bracket 33 is connected to the side frame 32. In another variation, the side frame 32 may also be disposed on the bottom frame 31 along the second axial direction L2, which is not limited thereto.

The reagent cassette transferring unit 4 is disposed on the rack unit 3 and includes two transferring rails 41, a transferring base 42, a driving module 43, and a heating module 44.

The transfer rails 41 are disposed on the bottom frame 31 along the second axial direction L2 at a distance.

The transfer base 42 has a base portion 421, a jig portion 422, and a jig sensor 423. The seat 421 is movably disposed between the transfer rails 41. The fixture portion 422 is detachably disposed on the seat portion 421, is suitable for installing the reagent cartridge 21, and has a through hole 424 communicating with the seat portion 421. The jig sensor 423 is installed on the seat body 421 for sensing whether the jig part 422 is located on the seat body 421.

In the present embodiment, the fixture 422 can be taken out from and put into the corresponding opening 321 of the side frame 32. In other variations, the jig 422 can be taken out from and put into the seat 421 along the second axial direction L2, and the side frames 32 may not have the openings 321, but not limited thereto.

The driving module 43 is used for driving the transferring seat 42 and moving the transferring seat 42 relative to the rack unit 3, and has a rack 431 mounted on the transferring seat 42, a gear 432 pivoted to the rack unit 3, and a driving member 433 for driving the gear 432. When the driving member 433 drives the gear 432 to rotate, the rack 431 drives the transferring seat 42, and the transferring seat 42 is moved along the second axial direction L2 on the transferring track 41, so that the stirring sleeves 221 can be sequentially corresponding to the reagent slots 211 of the reagent cassette 21. It should be noted that, in other variations of the present embodiment, a belt with a pulley may be replaced to achieve the purpose of driving, but not limited thereto.

The heating module 44 is disposed on the seat body 421 of the transfer seat 42 and corresponds to the through hole 424 of the fixture 422. When the operator mounts the reagent cartridges 21 on the fixture 422, one of the reagent slots 211 of each reagent cartridge 21 is adjacent to the corresponding heating module 44, and the heating module 44 heats the reagent, thereby increasing the efficiency of the specific biochemical reaction. In the present embodiment, the heating module 44 is a metal heating plate, but not limited thereto.

Each variable speed lifting unit 5 is connected to the frame unit 3 and includes a linear moving module 51, a variable speed transmission module 52, and a rotating module 53.

The linear moving module 51 is disposed on one of the extension brackets 33 of the rack unit 3, and has a guide rail 511 connected to the one of the extension brackets 33, and a slider 512 movably connected to the guide rail 511 along the first axial direction L1 and adapted to mount the stirring bar 22.

The slider 512 moves relative to the frame unit 3 between a top position (shown in fig. 4), a mid-point position (shown in fig. 5), and a bottom position (shown in fig. 6). The midpoint location is between the top location and the bottom location.

In the top position, the sliding block 512 is adapted to be away from the reagent cassette 21 and synchronously drives the bottom end of the stirring sleeve 221 away from the reagent cassette 21.

At the midpoint position, the sliding block 512 is adapted to drive the bottom end of the stirring sleeve 221 to be adjacent to the liquid level 214.

In the bottom position, the slide 512 is adapted to be adjacent to the reagent cartridge 21 and simultaneously drives the stirring sleeve 221 through the liquid surface 214 and adjacent to the bottom end of the reagent cartridge 21.

The variable speed transmission module 52 is connected between the linear moving module 51 and the rotating module 53, and has a transmission member 521 and a guide member 522. The transmission element 521 has a pivot portion 523 and a guide portion 524 disposed in opposite directions. The guide 522 is movably coupled to the guide 524. In the embodiment, the guiding portion 524 of the transmission member 521 is a long hole, and the guiding member 522 is in a shape of a circular rod and can rotatably slide in the guiding portion 524, but not limited thereto.

The rotation module 53 has a power member 531 and an output shaft 532 driven by the power member 531. The power member 531 is attached to one of the side frames 32 of the frame unit 3. The output shaft 532 is pivotally disposed on the power member 531 along the second axial direction L2, and can rotate in two directions and is connected to the speed changing transmission module 52. In the embodiment, the output shaft 532 is connected to the pivot portion 523 of the transmission member 521, and at this time, the guide member 522 is connected to the sliding block 512 of the linear moving module 51.

When the output shaft 532 drives the transmission member 521 to rotate, the guiding portion 524 of the transmission member 521 moves and rotates relative to the guiding member 522, and the sliding block 512 is driven to move along the first axial direction L1 along the guiding rail 511, and the stirring sleeve 221 is driven to move linearly at a variable speed.

It should be noted that, in the present embodiment, the rotating module 53 is a stepping motor, and is located on two opposite sides of the guide rail 511 along the third axial direction L3 and the guide 522. By means of the characteristics of the stepping motor, such as low cost, high torque, accurate positioning control of an open loop and the like, the variable speed transmission module 52 is favorably matched, and the advantage of stable variable speed movement is achieved.

Referring to fig. 7, in another variation of the present embodiment, the pivoting portion 523 'may be pivotally connected to the sliding block 512', and at this time, the guiding element 522 'is fixed to the frame unit 3', and the output shaft 532 'is cam-shaped and is used for pushing against the guiding portion 524' of the transmission element 521 ', so that the guiding portion 524' of the transmission element 521 'rotates and moves relative to the guiding element 522'. And is not limited thereto.

Referring to fig. 1, 3, and 8, the row fixing unit 6 is disposed on the linear moving module 51 of one of the variable-speed lifting units 5, and includes a row fixing seat 61 disposed on the sliding block 512 and adapted to mount the stirring row 22, and a row sensor 62.

In the present embodiment, the stirring bar 22 is taken out and put in from the opening 321 of the corresponding side frame 32, and the bar sensor 62 is used to sense whether the stirring bar 22 is actually mounted on the bar fixing seat 61.

It should be noted that, in other variations of the present embodiment, the stirring sleeves 221 may also be separately disposed and respectively mounted on the row fixing seat 61. At this time, each stirring sheath 221 is first placed in one of the reagent vessels 211 of the respective reagent cartridge 21. The row of fixing seats 61 is adapted to connect and fix the respective stirring sleeves 221 from the corresponding reagent tanks 211 along the first axial direction L1, but not limited thereto.

Referring to fig. 1, 3, and 9, the magnetic bead transferring unit 7 is disposed on the linear moving module 51 of the other variable-speed lifting unit 5 with respect to the row fixing unit 6, and includes a magnetic bar frame 71 connected to the corresponding sliding block 512, and four magnetic bars 72 disposed corresponding to the stirring sleeve 221.

In the present embodiment, the bar magnet holder 71 and the corresponding slider 512 are integrally formed, but in other variations, the bar magnet holder can be manufactured separately and then assembled, and the invention is not limited thereto.

Each magnetic rod 72 is inserted into the respective stirring sleeve 221, and has a rod portion 721 connected to the magnetic rod frame 71 and a magnetic portion 722 adjacent to the bottom of the stirring sleeve 221. When the sliding blocks 512 of the variable speed lifting unit 5 are all located at the bottom position, the magnetic portion 722 is adapted to magnetically attach the magnetic beads 212 to the stirring sleeve 221 (as shown in fig. 9).

The sterilizing unit 8 includes a UV lamp 81 disposed on the rack unit 3, and in this embodiment, the UV lamp 81 is disposed adjacent to the reagent cassette transferring unit 4 and the magnetic bead transferring unit 7.

With the above structure, the operation procedure of the present embodiment applied to the biochemical reaction of nucleic acid extraction is described as follows:

in the first step, the operator prepares the unused reagent cassettes 21 and the stirring row member 22, and correspondingly holds a plurality of biological samples S to be tested in one of the reagent tanks 211 of different reagent cassettes 21.

And step two, one of the variable speed lifting units 5 drives the row piece fixing unit 6 to descend, and an operator fixes the stirring row piece 22 on the row piece fixing seat 61. Then, the one variable-speed lifting unit 5 drives the row fixing unit 6 to lift up and move away from the bottom frame 31, so that the operator can fix the reagent cassette 21 on the fixture portion 422 of the transfer seat 42 first, and then fix the fixture portion 422 and the reagent cassette 21 on the seat body portion 421 of the transfer seat 42. At the same time, the tool sensor 423 of the transferring seat 42 and the component arrangement sensor 62 of the component arrangement fixing unit 6 detect whether both are installed correctly, and then perform the subsequent biochemical reaction, thereby achieving a safe operation mechanism.

The following steps three to six are to describe how to perform the reaction procedures of lysis, washing, nucleic acid recovery, etc. on the biological sample S sequentially in the reagent tank 211 with the magnetic beads 212 and the reagents 213 with different properties.

Step three, firstly, the cell wall and the cell membrane of the biological sample S are separated and destroyed in the reagent 213 with the cell tissue lysis effect by matching with the magnetic beads 212, so as to release the nucleic acid therein. In this embodiment, the stirring sleeve 221 is driven by the one variable speed lifting unit 5 to perform short-distance stirring at up-down variable speed in the reagent tank 211, thereby achieving the effects of homogenizing and shortening the reaction time. It should be noted that the slide block 512 of the other variable speed lifting unit 5 is continuously located at the top end position and links the magnetic rod 72 away from the stirring sleeve 221 while stirring is performed.

In addition, it should be noted that the present embodiment also has the following functions: when the slide block 512 of the linear moving module 51 is located at the midpoint position (as shown in fig. 5), the slide block is adapted to drive the bottom end of the stirring sleeve 221 to be adjacent to the liquid level 214, and at this time, the distance between the guide member 522 and the output shaft 532 is reduced, so that the moving speed of the slide block 512 is reduced, thereby preventing the reagent 213 or the biological sample S from splashing when the stirring sleeve 221 breaks through the liquid level 214, and further reducing the generation of pollution. That is, the instantaneous velocity of the slider 512 moving to the midpoint position (shown in FIG. 5) is slower than the instantaneous velocity moving to the positions adjacent to the top position (shown in FIG. 4) and the bottom position (shown in FIG. 6). However, in other variations of the present embodiment, the instantaneous speed relationship between different positions can be changed by adjusting the relative positions of the guide 522 and the output shaft 532 in the first axial direction L1, and therefore, the present invention is not limited thereto.

Step four, the homogenized biological sample S and the separated nucleic acids are attached to the magnetic beads 212, and at this time, as shown in fig. 9, the magnetic bead transfer unit 7 is driven by the other variable speed lifting unit 5, so as to drive each magnetic rod 72 to penetrate through the respective stirring sleeve 221, and the magnetic portion 722 of each magnetic rod 72 is adjacent to the bottom end of the respective stirring sleeve 221, so that the magnetic beads 212 are adsorbed outside the stirring sleeve 221. Then, the variable speed lifting unit 5 synchronously drives the stirring sleeve 221 and the magnetic rod 72 to ascend, and is away from the reagent cartridge 21. It should be noted that in the present embodiment, the agitating sleeve 221 and the magnetic rod 72 are raised at a constant speed by providing two variable speed raising and lowering units 5 having the same structure, but in another modification, the variable speed raising and lowering unit 5 for driving the magnetic rod 72 may be replaced with another driving method such as a belt, a screw, or an interlocking mechanism, but the invention is not limited thereto.

Step five, after the reagent cassette transfer unit 4 drives the reagent cassette 21 to transfer the reagent cassette along the second axial direction L2 and the other reagent tank 211 of the reagent cassette 21 is positioned below the stirring sleeve 221, the stirring and transferring procedure of step three and step four is repeated. At this time, the reagent 213 for removing other non-nucleic acid substances is used in combination, and the reagent is repeatedly washed in different reagent tanks 211, so as to achieve the purpose of purification and extraction. In addition, in the present embodiment, the heating module 44 can be used to heat a specific biochemical reaction, thereby increasing the reaction efficiency.

Step six, the reagent 213 accommodated in the last reagent well 211 is used for precipitating the nucleic acid of the biological sample S from the magnetic beads 212. Then, the other variable-speed lifting unit 5 is driven to drive the magnetic rod 72 to move away from the reagent cassette 21, so that the operator can take out the reagent cassette 21 with biochemical reaction completed from the present embodiment.

And step seven, after the operator takes out the stirring row piece 22, starting the sterilizing unit 8, and sterilizing the reagent cassette transfer unit 4 and the magnetic bead transfer unit 7 by ultraviolet light, thereby further reducing the pollution.

The test results of this example are summarized in tables 1 and 2 below. The conventional extraction apparatus is, as described in the background art, configured such that a screw drives a stirring sleeve to move up and down at a constant speed. The test result is that under the condition of the same extraction reagent, the same vibration frequency and the same vibration amplitude, nucleic acid extraction experiments are respectively carried out twice aiming at two biological samples S, and the average value of the nucleic acid concentration (the unit is ng/mu l) is taken for carrying out comparison of the nucleic acid extraction recovery rate; the recovery rate of this example was calculated with the recovery rate of the conventional extraction apparatus as 100%. The absorbance ratio (OD260/OD280 ratio) represents the purity of nucleic acid extraction, and when the absorbance ratio is greater than 1.6, it indicates that the better extraction purity has been achieved.

Table 1. test with 200 μ l of human blood:

table 2 test with 40mg chicken spleen tissue:

by means of the above structure and operation procedure, the present embodiment has the following advantages:

firstly, when the cell wall and the cell membrane of the biological sample S are separated and destroyed in the reagent 213 with the cell tissue lysis effect, the stirring sleeve 221 is driven by one of the variable speed lifting units 5 to move at variable speed, so that the magnetic beads 212 contained in the reagent tank 211 can be more powerfully stirred, a better grinding effect is achieved, and the proportion of the released nucleic acid is further improved.

Secondly, the biological sample S after the homogeneous lysis needs to be further washed repeatedly to remove other non-nucleic acid substances. By driving the stirring sleeve 221 to move at a variable speed, the turbulence intensity (turbulence intensity) in the reagent 213 is increased, and an excellent mixing and stirring effect is further achieved, so that the stirring uniformity can be improved, the heat energy transfer is facilitated, and the stirring time is shortened.

Thirdly, by means of the variable speed transmission module 52, not only the overall volume and weight are reduced compared with the prior art to achieve better portability, but also a better mixing and stirring effect can be achieved, so that the programming requirement for the rotation module 53 can be simplified, a high-price controller required by complex programming is not required to be purchased, the overall required cost is further reduced, and the portability is facilitated.

Fourthly, compared with the high power consumption characteristic of the existing biochemical reactor, the present embodiment uses the variable speed transmission module 52 to rotate the output shaft 532 of the rotation module 53 for a very small number of turns, so as to achieve the purpose of stirring, and greatly reduce the power consumption in use, which is beneficial to the feasibility of non-plug operation (driven by a battery), and is further suitable for being applied to the first line outside the laboratory, thereby achieving better convenience and popularization.

In conclusion, the object of the present invention can be achieved.

However, the above embodiments are only examples of the present invention, and the scope of the present invention should not be limited thereto, and all the simple equivalent changes and modifications made according to the claims and the contents of the specification should be included in the scope of the present invention.

Claims (10)

1. A portable biochemical reactor adapted to drive at least one agitator sleeve to displace relative to at least one reagent cartridge along a first axial direction, the portable biochemical reactor comprising a housing unit, characterized in that:

the portable biochemical reactor also comprises at least one variable speed lifting unit;

the rack unit is suitable for holding the at least one reagent box; and

the at least one variable speed lift unit comprises:

a linear movement module having a guide rail connected to the rack unit, and a slide movably connected to the guide rail along the first axial direction and adapted to mount the at least one stirring sheath, the slide being movable relative to the rack unit between a top position, in which the slide is adapted to be remote from the at least one reagent cartridge, and a bottom position, in which the slide is adapted to be adjacent to the at least one reagent cartridge,

a variable speed drive module having a drive member and a guide member, the drive member having a pivot portion and a guide portion arranged in opposite directions, the guide member being movably connected to the guide portion, one of the guide member and the pivot portion of the drive member being connected to the slider, an

And the rotating module is connected to the other one of the guide part and the pivoting part of the transmission part and is used for driving the transmission part to rotate, so that the transmission part drives the sliding block to move along the guide rail along the first axial direction through the guide part in the rotating process, and synchronously drives the at least one stirring sleeve to generate linear movement with variable speed.

2. The portable biochemical reactor according to claim 1, wherein: the at least one reagent cartridge is filled with a reagent and defines a liquid level, the slide is further movable relative to the rack unit to a midpoint position between the top end position and the bottom end position, the slide is adapted to drive the bottom end of the at least one stirring jacket to be adjacent to the liquid level at the midpoint position, and an instantaneous speed of the slide moving to the midpoint position is slower than an instantaneous speed of the slide moving to the position adjacent to the top end position.

3. The portable biochemical reactor according to claim 1, wherein: and the rotating module and the guide piece are positioned on two opposite sides of the guide rail along the third axial direction and are provided with a power piece and an output shaft, the power piece is connected to the rack unit, the output shaft is pivoted on the power piece along the second axial direction and is connected to the other one of the guide piece and the pivoting part of the transmission piece relative to the linear moving module.

4. The portable biochemical reactor according to claim 1, wherein: the sliding block is connected to the guide piece, and the pivoting part of the transmission piece is fixed to the rotating module.

5. The portable biochemical reactor according to claim 1, wherein: the guide part of the transmission part is a long hole, and the guide part slides in the guide part.

6. The portable biochemical reactor according to claim 1, wherein: the portable biochemical reactor also comprises a reagent box transferring unit, and a second axial direction orthogonal to the first axial direction is defined, wherein the reagent box transferring unit comprises two transferring rails arranged on the rack unit along the second axial direction, a transferring seat movably arranged on the transferring rails, and a driving module used for driving the transferring seat to move relative to the rack unit.

7. The portable biochemical reactor according to claim 6, wherein: the driving module is provided with a rack arranged on the load-moving seat, a gear pivoted on the rack unit and a driving piece for driving the gear.

8. The portable biochemical reactor according to claim 6, wherein: the rack unit comprises an underframe and side frames, the side frames are arranged on the underframe and are provided with openings, the reagent box transferring and carrying seat of the reagent box transferring and carrying unit is provided with a seat body part arranged on the transferring and carrying track and a jig part which can be detachably connected with the seat body part, and the jig part is used for installing the at least one reagent box and can be taken out from and put into the openings of the side frames.

9. The portable biochemical reactor according to claim 6, wherein: the reagent box transferring unit further comprises a heating module, the transferring seat is provided with a seat body part and a jig part, the seat body part is arranged on the transferring track and is used for installing the heating module, the jig part can be detachably connected with the seat body part and is used for installing the at least one reagent box, and the jig part is provided with a through hole corresponding to the heating module.

10. The portable biochemical reactor according to claim 1, wherein: the portable biochemical reactor further comprises a magnetic bead transferring unit, the portable biochemical reactor comprises two variable speed lifting units, a plurality of magnetic beads are loaded in the at least one reagent box, a sliding block of one variable speed lifting unit is connected to the stirring sleeve, the other variable speed lifting unit is connected to the magnetic bead transferring unit, the magnetic bead transferring unit comprises at least one magnetic rod inserted in the at least one stirring sleeve, the at least one magnetic rod is provided with a magnetic part adjacent to the bottom of the stirring sleeve, and when the sliding block is located at the bottom end position, the magnetic part is suitable for magnetically adsorbing the magnetic beads on the stirring sleeve.

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