CN214830280U - Rotary stirring formula nucleic acid extraction element - Google Patents
Rotary stirring formula nucleic acid extraction element Download PDFInfo
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- CN214830280U CN214830280U CN202023313456.3U CN202023313456U CN214830280U CN 214830280 U CN214830280 U CN 214830280U CN 202023313456 U CN202023313456 U CN 202023313456U CN 214830280 U CN214830280 U CN 214830280U
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Abstract
The utility model relates to a nucleic acid extraction technical field provides a rotatory stirring formula nucleic acid extraction element. The extraction and blending device comprises an extraction and blending module and a portal frame, the extraction and blending module is arranged on the portal frame, and the extraction and blending module can move up and down on the portal frame to realize the blending and extraction of the extraction and blending module on the nucleic acid sample; the extraction and uniform mixing module also comprises a magnetic rod sleeve movement structure and a guide rail; the two ends of the magnetic rod sleeve moving structure are provided with the sliding block connecting units which are used for being connected with the guide rail, so that the magnetic rod sleeve moving structure can ascend or descend on the guide rail.
Description
Technical Field
The application relates to the technical field of nucleic acid extraction and purification, in particular to a rotary stirring type nucleic acid extraction device.
Background
Nucleic acid is a carrier of genetic materials in animal and plant cells and microorganisms, and is one of the most basic materials constituting life. With the development of modern molecular biology technology, research and analysis on nucleic acid have been widely applied in the fields of clinical diagnosis, food safety, animal husbandry and the like.
Since biological cells contain other substances such as nucleic acids, proteins, and polysaccharides, the nucleic acids need to be separately studied and analyzed, and the nucleic acids need to be extracted and separated from the complex biological environment in which the cells are located through steps such as lysis, adsorption, washing, and elution.
The common methods for extracting nucleic acid in laboratories are mainly two types, manual extraction and extraction by a nucleic acid extraction instrument. Manual extraction is gradually eliminated due to complex operation, long time, easy error and the like, and rapid and high-flux nucleic acid extraction instrument lifting becomes a mainstream demand. The existing nucleic acid extraction instruments on the market are mainly divided into an upper magnetic attraction method and a lower magnetic attraction method, and the lower magnetic attraction method has the factors of long total reaction time, complex temperature control and the like; most of nucleic acid extraction instruments adopting the magnetic attraction method on the market uniformly mix and stir cell solutions by enabling the magnetic rod sleeve to move up and down quickly, the mode is low in mixing speed and poor in mixing effect, and meanwhile, the problems of magnetic bead loss, cross contamination and the like are caused due to the phenomenon that liquid splashes caused by the up-and-down movement of the magnetic rod sleeve.
Therefore, how to realize high efficiency of uniform mixing and reduce cross contamination is a technical problem which needs to be solved urgently in the current nucleic acid extraction and purification.
Disclosure of Invention
Therefore, how to solve and draw the mixing inefficiency and have cross contamination's problem, the utility model provides a rotatory stirring formula nucleic acid extraction element's extraction mixing device, include:
a portal frame and an extraction and uniform mixing module;
the extraction and blending module is arranged on the portal frame and moves up and down on the portal frame so as to extract and blend the sample;
the extraction and uniform mixing module comprises a magnetic bar sleeve movement structure and a guide rail;
the magnetic rod sleeve moving structure is connected with the guide rail through the sliding block connecting units arranged at the two ends of the magnetic rod sleeve moving structure so as to realize ascending or descending on the guide rail.
Description of technical effects: the extraction and blending device comprises an extraction and blending module and a portal frame, the extraction and blending module is arranged on the portal frame, and the extraction and blending module can move up and down on the portal frame to realize the blending and extraction of the extraction and blending module on the nucleic acid sample; the extraction and uniform mixing module also comprises a magnetic rod sleeve movement structure and a guide rail; the two ends of the magnetic rod sleeve moving structure are provided with the sliding block connecting units which are used for being connected with the guide rail, so that the magnetic rod sleeve moving structure can ascend or descend on the guide rail.
The two ends of the magnetic bar sleeve movement structure are provided with sliding block connecting units used for being connected with the guide rails; the slide block connecting unit is connected with the guide rail in a sliding mode, and therefore ascending or descending of the magnetic rod sleeve movement structure is achieved.
Specifically, the guide rail is arranged on the portal frame in parallel and comprises a movable slide block unit;
the slider unit is used for being connected with the slider connecting unit so as to realize the sliding connection between the magnetic rod sleeve movement structure and the guide rail and further realize the ascending or descending of the magnetic rod sleeve movement structure.
Description of technical effects: the guide rail is provided with a slide block which is connected with a slide block connecting unit so as to realize the sliding connection of the magnetic rod sleeve movement structure and the guide rail and further realize the ascending or descending of the magnetic rod sleeve movement structure.
It should be further explained that the guide rails are arranged in parallel on the gantry, and at least two ends of the magnetic rod sleeve moving structure are connected with the guide rails, so that the stability in the ascending or descending process can be ensured.
Specifically, the slide block connecting unit comprises a first slide block connecting unit and a second slide block connecting unit which are positioned at two ends of the magnetic rod sleeve movement structure;
the first sliding block connecting unit and the second sliding block connecting unit are respectively in sliding connection with a first sliding block and a second sliding block which are arranged on the guide rail.
Description of technical effects: the sliding connection between the sliding block and the sliding block is realized through the connection between the sliding block connecting unit and the sliding block. The slide block connecting units at the two ends of the magnetic rod sleeve moving structure are connected with the first slide block and the second slide block and used for realizing the ascending or descending of the magnetic rod sleeve moving structure. The first sliding block and the second sliding block are respectively arranged on the two parallel guide rails.
An embodiment is provided in which the bar magnet housing movement structure comprises three ends, and thus three slider connection units are provided, corresponding to three sliders and three parallel guide rails.
In another embodiment, the bar magnet sleeve moving structure comprises four ends, and therefore, four sliding block connecting units are arranged, so that the four sliding blocks correspond to four sliding blocks and four guide rails arranged in parallel.
The magnetic bar motion structure comprises a third slide block connecting unit and a fourth slide block connecting unit which are positioned at two ends of the magnetic bar motion structure;
the third slider connecting unit and the fourth slider connecting unit are respectively connected with the third slider and the fourth slider arranged on the guide rail in a sliding manner so as to realize the ascending or descending of the magnetic bar moving structure on the guide rail.
Description of technical effects: two slide block connecting units are arranged at two ends of the magnetic bar moving structure and are respectively connected with the third slide block and the fourth slide block on the guide rail, so that the magnetic bar moving structure can ascend or descend on the guide rail.
An embodiment is provided, wherein the guide rails where the third and fourth sliders are respectively connected with the two slider connecting units at the two ends of the magnetic rod movement structure are the same guide rail where the first slider and the second slider are respectively located.
In another embodiment, the guide rail connected with the bar magnet sleeve moving structure is the upper half part of the guide rail, and the guide rail connected with the bar magnet sleeve moving structure is the lower half part of the guide rail.
Specifically, the extraction and blending module further comprises a magnetic bar motion motor; the magnetic rod movement motor is used for connecting the magnetic rod sleeve movement structure and the magnetic rod movement structure and driving the magnetic rod sleeve movement structure and the magnetic rod movement structure to be relatively close to or far away from each other on the guide rail.
Description of technical effects: and the magnetic bar motion motor is used for connecting the magnetic bar sleeve motion structure and the magnetic bar motion mechanism and driving the magnetic bar sleeve motion structure and the magnetic bar motion mechanism to be relatively close to or far away from each other.
Specifically, the magnetic rod movement structure further comprises a magnetic rod sleeve lifting motor; the magnetic rod sleeve lifting motor is used for connecting the magnetic rod movement structure and the magnetic rod sleeve movement structure and driving the magnetic rod movement structure and the magnetic rod sleeve movement structure to synchronously ascend or descend on the guide rail.
Description of technical effects: and the magnetic rod sleeve lifting motor is used for connecting the magnetic rod movement structure and the magnetic rod sleeve movement structure and driving the magnetic rod movement structure and the magnetic rod sleeve movement structure to synchronously ascend or descend on the guide rail.
It should also be noted that the magnetic rod movement structure and the magnetic rod sleeve movement structure driven by the magnetic rod movement motor and the magnetic rod sleeve lifting motor both move along a straight line and just keep the same with the rail movement mode, so that the reduction of different parts of the whole structure is realized, and unnecessary space waste is reduced.
Specifically, the magnetic rod sleeve movement structure further comprises a magnetic rod sleeve rotating motor; the magnetic rod sleeve rotating motor is installed on the magnetic rod sleeve moving structure and used for driving the magnetic rod sleeve to rotate so as to uniformly mix the sample and the magnetic bead mixture.
In particular, the number of said guides is two.
An embodiment is provided here, the number of guide rails being 3 or 4.
The utility model also provides a rotatory stirring formula nucleic acid extraction element, include:
extracting a blending device and a deep hole plate moving device;
the blending device comprises a portal frame and an extraction blending module; the extraction and blending module is arranged on the portal frame and moves up and down on the portal frame so as to extract and blend the sample;
the deep hole plate moving device is provided with a deep hole plate and a deep hole plate limiting structure;
the deep hole plate is used for placing a sample to be detected, magnetic beads for reaction and a reagent;
the deep hole plate limiting structure is used for fixing the deep hole plate on the deep hole plate moving device.
Description of technical effects: the utility model provides a rotary stirring type nucleic acid extraction device, which comprises an extraction and mixing device and a deep hole plate moving device; the blending device comprises a portal frame and an extraction blending module; the extraction and blending module is arranged on the portal frame and moves up and down on the portal frame so as to extract and blend the sample; the deep hole plate moving device is provided with a deep hole plate and a deep hole plate limiting structure; the deep hole plate is used for placing a sample to be detected, magnetic beads for reaction and a reagent; the deep hole plate limiting structure is used for fixing the deep hole plate on the deep hole plate moving device.
Specifically, the deep hole plate moving device further comprises a heating structure for heating the deep hole plate; the heating structure is arranged below the deep hole plate limiting structure so as to meet the temperature requirement during nucleic acid extraction reaction.
Specifically, the deep hole plate moving device further comprises a stage moving structure and a deep hole plate stage structure;
the platform deck moving structure is provided with two parallel guide rails;
the deep hole plate carrying platform structure is arranged on the two guide rails and moves along the guide rails.
The utility model provides a pair of rotatory stirring formula nucleic acid extraction element. The extraction and blending device comprises an extraction and blending module and a portal frame, the extraction and blending module is arranged on the portal frame, and the extraction and blending module can move up and down on the portal frame to realize the blending and extraction of the extraction and blending module on the nucleic acid sample; the extraction and uniform mixing module also comprises a magnetic rod sleeve movement structure and a guide rail; the two ends of the magnetic rod sleeve moving structure are provided with the sliding block connecting units which are used for being connected with the guide rail, so that the magnetic rod sleeve moving structure can ascend or descend on the guide rail.
Drawings
FIG. 1 is a schematic view of the overall structure of a rotary stirring type nucleic acid isolation apparatus according to an embodiment;
FIG. 2 is a block diagram of an extraction and mixing module of a rotary stirring type nucleic acid extraction apparatus according to an embodiment;
fig. 3 is a schematic structural diagram of a deep hole plate moving assembly having three deep hole plates according to an embodiment.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
Furthermore, in the description of the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and the like are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; the two elements may be directly connected or indirectly connected through an intermediate medium, or may be communicated with each other inside the two elements, or may be wirelessly connected or wired connected. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Furthermore, the technical features mentioned in the different embodiments of the invention described below can be combined with each other as long as they do not conflict with each other.
In order to achieve the above purpose, the utility model adopts the following scheme:
example (b):
as shown in fig. 1 and 2:
the extraction and blending device 1 comprises a portal frame 11, a magnetic rod sleeve movement structure 12, a magnetic rod movement mechanism 13 and a magnetic rod sleeve rotation mechanism 14.
The two ends of the main structure (left and right) of the magnetic rod sleeve moving structure 12 are respectively connected with the lower half part sliding blocks of the two sets of guide assemblies 111 on the portal frame 11, the magnetic rod sleeve lifting screw motor 121 fixed on the portal frame drives the mechanism to move up and down along the guide assemblies in the vertical direction, a plurality of magnetic rod sleeve loading mechanisms 123 are arranged on the magnetic rod sleeve moving structure 12, the magnetic rod sleeve loading mechanisms 123 are of hollow structures, and each loading mechanism processes a sample needing to extract nucleic acid.
The utility model provides a rotatory stirring formula nucleic acid extraction element's extraction mixing device, include:
a portal frame and an extraction and uniform mixing module;
the extraction and blending module is arranged on the portal frame and moves up and down on the portal frame so as to extract and blend the sample;
the extraction and uniform mixing module comprises a magnetic bar sleeve movement structure and a guide rail;
the magnetic rod sleeve moving structure is connected with the guide rail through the sliding block connecting units arranged at the two ends of the magnetic rod sleeve moving structure so as to realize ascending or descending on the guide rail.
Description of technical effects: the extraction and blending device comprises an extraction and blending module and a portal frame, the extraction and blending module is arranged on the portal frame, and the extraction and blending module can move up and down on the portal frame to realize the blending and extraction of the extraction and blending module on the nucleic acid sample; the extraction and uniform mixing module also comprises a magnetic rod sleeve movement structure and a guide rail; the two ends of the magnetic rod sleeve moving structure are provided with the sliding block connecting units which are used for being connected with the guide rail, so that the magnetic rod sleeve moving structure can ascend or descend on the guide rail.
The two ends of the magnetic bar sleeve movement structure are provided with sliding block connecting units used for being connected with the guide rails; the slide block connecting unit is connected with the guide rail in a sliding mode, and therefore ascending or descending of the magnetic rod sleeve movement structure is achieved.
Specifically, the guide rail is arranged on the portal frame in parallel and comprises a movable slide block unit;
the slider unit is used for being connected with the slider connecting unit so as to realize the sliding connection between the magnetic rod sleeve movement structure and the guide rail and further realize the ascending or descending of the magnetic rod sleeve movement structure.
Description of technical effects: the guide rail is provided with a slide block which is connected with a slide block connecting unit so as to realize the sliding connection of the magnetic rod sleeve movement structure and the guide rail and further realize the ascending or descending of the magnetic rod sleeve movement structure.
It should be further explained that the guide rails are arranged in parallel on the gantry, and at least two ends of the magnetic rod sleeve moving structure are connected with the guide rails, so that the stability in the ascending or descending process can be ensured.
Specifically, the slide block connecting unit comprises a first slide block connecting unit and a second slide block connecting unit which are positioned at two ends of the magnetic rod sleeve movement structure;
the first sliding block connecting unit and the second sliding block connecting unit are respectively in sliding connection with a first sliding block and a second sliding block which are arranged on the guide rail.
Description of technical effects: the sliding connection between the sliding block and the sliding block is realized through the connection between the sliding block connecting unit and the sliding block. The slide block connecting units at the two ends of the magnetic rod sleeve moving structure are connected with the first slide block and the second slide block and used for realizing the ascending or descending of the magnetic rod sleeve moving structure. The first sliding block and the second sliding block are respectively arranged on the two parallel guide rails.
An embodiment is provided in which the bar magnet housing movement structure comprises three ends, and thus three slider connection units are provided, corresponding to three sliders and three parallel guide rails.
In another embodiment, the bar magnet sleeve moving structure comprises four ends, and therefore, four sliding block connecting units are arranged, so that the four sliding blocks correspond to four sliding blocks and four guide rails arranged in parallel.
An embodiment is provided, wherein two sets of parallel linear guide rails are erected in the vertical direction of the portal frame 11 to serve as the guide assembly 111, the guide assembly stops supplying and guiding for the movement of the subsequent structural member in the vertical direction, and 2 sliding blocks are arranged on each 1 guide rail to divide the guide assembly into an upper half part and a lower half part.
The magnetic bar motion structure comprises a third slide block connecting unit and a fourth slide block connecting unit which are positioned at two ends of the magnetic bar motion structure;
the third slider connecting unit and the fourth slider connecting unit are respectively connected with the third slider and the fourth slider arranged on the guide rail in a sliding manner so as to realize the ascending or descending of the magnetic bar moving structure on the guide rail.
Description of technical effects: two slide block connecting units are arranged at two ends of the magnetic bar moving structure and are respectively connected with the third slide block and the fourth slide block on the guide rail, so that the magnetic bar moving structure can ascend or descend on the guide rail.
An embodiment is provided, wherein the guide rails where the third and fourth sliders are respectively connected with the two slider connecting units at the two ends of the magnetic rod movement structure are the same guide rail where the first slider and the second slider are respectively located.
In another embodiment, the guide rail connected with the bar magnet sleeve moving structure is the upper half part of the guide rail, and the guide rail connected with the bar magnet sleeve moving structure is the lower half part of the guide rail.
According to one embodiment, the magnetic rod moving mechanism 13 is provided with a plurality of magnetic rods 131, the number and the position of the magnetic rods correspond to those of the magnetic rod sleeve loading mechanisms 123 one by one, and the two ends (left and right) of the main structure of the magnetic rod moving mechanism are respectively connected with the upper half part of the sliding blocks of the two sets of guide assemblies 111 on the portal frame 11.
Specifically, the extraction and blending module further comprises a magnetic bar motion motor; the magnetic rod movement motor is used for connecting the magnetic rod sleeve movement structure and the magnetic rod movement structure and driving the magnetic rod sleeve movement structure and the magnetic rod movement structure to be relatively close to or far away from each other on the guide rail.
Description of technical effects: and the magnetic bar motion motor is used for connecting the magnetic bar sleeve motion structure and the magnetic bar motion mechanism and driving the magnetic bar sleeve motion structure and the magnetic bar motion mechanism to be relatively close to or far away from each other.
Specifically, the magnetic rod movement structure further comprises a magnetic rod sleeve lifting motor; the magnetic rod sleeve lifting motor is used for connecting the magnetic rod movement structure and the magnetic rod sleeve movement structure and driving the magnetic rod movement structure and the magnetic rod sleeve movement structure to synchronously ascend or descend on the guide rail.
Specifically, the magnetic rod sleeve movement structure further comprises a magnetic rod sleeve rotating motor; the magnetic rod sleeve rotating motor is installed on the magnetic rod sleeve moving structure and used for driving the magnetic rod sleeve to rotate so as to uniformly mix the sample and the magnetic bead mixture.
Description of technical effects: and the magnetic rod sleeve lifting motor is used for connecting the magnetic rod movement structure and the magnetic rod sleeve movement structure and driving the magnetic rod movement structure and the magnetic rod sleeve movement structure to synchronously ascend or descend on the guide rail.
It should also be noted that the magnetic rod movement structure and the magnetic rod sleeve movement structure driven by the magnetic rod movement motor and the magnetic rod sleeve lifting motor both move along a straight line and just keep the same with the rail movement mode, so that the reduction of different parts of the whole structure is realized, and unnecessary space waste is reduced.
An embodiment is provided, and the magnetic rod motion lead screw motor 132 fixed on the magnetic rod sleeve motion structure 12 is connected with the magnetic rod sleeve motion structure 12, and can move together with the magnetic rod sleeve motion structure (12), and independently, the magnetic rod motion lead screw motor 132 can drive the mechanism to move along the guide component 111, and move up and down in the vertical direction relative to the magnetic rod sleeve motion structure 12, and when moving down relative to the magnetic rod sleeve motion structure 12, the magnetic rod 131 on the structure can extend out a certain distance through the hollow structure on the magnetic rod sleeve loading mechanism 123; the bar magnet sleeve rotating mechanism 14 is installed on the bar magnet sleeve moving structure 12 and can move up and down along with the bar magnet sleeve moving structure 12, and the bar magnet sleeve rotating motor 141 on the mechanism can drive the plurality of bar magnet sleeve loading mechanisms 123 to rotate simultaneously.
In particular, the number of said guides is two.
An embodiment is provided here, the number of guide rails being 3 or 4.
As shown in figure 3 of the drawings,
the deep hole plate moving assembly 2 comprises a stage moving mechanism 21 and a deep hole plate stage assembly 22;
two parallel guide rails perpendicular to the extracting and blending device are arranged in the carrier moving mechanism 21 as a guide assembly 211, and the guide assembly 221 passes through the lower part of the extracting and blending device 1; the deep hole plate carrier assembly 11 is arranged on two guide assemblies 211 and driven by a carrier moving motor 212 to move along the guide assemblies, a plurality of deep hole plate limiting mechanisms 221 are arranged on the deep hole plate carrier assembly 1 and used for positioning the loaded deep hole plate, and a heating assembly 222 is arranged below each deep hole plate limiting mechanism 221 and used for heating the deep hole plate according to the temperature required by the nucleic acid extraction reaction;
the utility model also provides a rotatory stirring formula nucleic acid extraction element, include:
extracting a blending device and a deep hole plate moving device;
the blending device comprises a portal frame and an extraction blending module; the extraction and blending module is arranged on the portal frame and moves up and down on the portal frame so as to extract and blend the sample;
the deep hole plate moving device is provided with a deep hole plate and a deep hole plate limiting structure;
the deep hole plate is used for placing a sample to be detected, magnetic beads for reaction and a reagent;
the deep hole plate limiting structure is used for fixing the deep hole plate on the deep hole plate moving device.
Description of technical effects: the utility model provides a rotary stirring type nucleic acid extraction device, which comprises an extraction and mixing device and a deep hole plate moving device; the blending device comprises a portal frame and an extraction blending module; the extraction and blending module is arranged on the portal frame and moves up and down on the portal frame so as to extract and blend the sample; the deep hole plate moving device is provided with a deep hole plate and a deep hole plate limiting structure; the deep hole plate is used for placing a sample to be detected, magnetic beads for reaction and a reagent; the deep hole plate limiting structure is used for fixing the deep hole plate on the deep hole plate moving device.
Specifically, the deep hole plate moving device further comprises a heating structure for heating the deep hole plate; the heating structure is arranged below the deep hole plate limiting structure so as to meet the temperature requirement during nucleic acid extraction reaction.
Specifically, the deep hole plate moving device further comprises a stage moving structure and a deep hole plate stage structure;
the platform deck moving structure is provided with two parallel guide rails;
the deep hole plate carrying platform structure is arranged on the two guide rails and moves along the guide rails.
The specific implementation mode of the accounting extraction is as follows:
adding a biological cell sample of which nucleic acid needs to be extracted into a reagent of a specified hole site, inserting a magnetic rod sleeve 4 into a hole of a deep-hole plate 3, which is pre-filled with magnetic beads, inputting an instruction to enable a platform moving mechanism 21 to drive a deep-hole plate platform assembly 22 to move to a loading working position, and placing the deep-hole plate 3 inserted into the magnetic rod sleeve 4 on a deep-hole plate limiting mechanism 221 on the platform moving mechanism 21 by an operator; the carrying platform moving mechanism 21 moves inwards to enable the magnetic rod sleeve 4 in the deep hole plate 3 to be aligned with the corresponding magnetic rod sleeve loading mechanism 123, the magnetic rod sleeve moving mechanism 12 moves downwards to a loading position, the magnetic rod sleeve loading mechanism 123 is connected and fixed with the magnetic rod sleeve 4, the magnetic rod moving mechanism 13 moves downwards relative to the magnetic rod sleeve moving mechanism 12 to a magnetic attraction working position, namely the magnetic rod extends into the magnetic rod sleeve 4 and stands until magnetic beads are adsorbed on the surface of the magnetic rod sleeve 4 under the action of a magnetic field, the magnetic rod sleeve moving mechanism 12 moves upwards to a waiting position, the carrying platform moving mechanism 21 moves until a hole position where a sample is located in the deep hole plate 3 is aligned with the magnetic rod sleeve 4, the magnetic rod sleeve moving mechanism 12 moves downwards to a stirring working position, the magnetic rod moving mechanism 13 moves upwards relative to the magnetic rod sleeve moving mechanism 12 to enable the magnetic beads on the surface of the magnetic rod sleeve to be separated from the external magnetic field and fall into a solution where the sample is located, the magnetic rod sleeve rotating mechanism 14 drives the magnetic rod sleeve 4 to rotate and the magnetic beads, Sample solution is stirred and mixed uniformly, the magnetic rod sleeve motion structure 12 moves up and down in a small range at a specified frequency to improve the mixing rate, nucleic acid and magnetic beads are specifically combined and adsorbed on the magnetic beads in the process, the magnetic rod sleeve motion structure 12 moves to a stirring working position after stirring is finished, the magnetic rod motion mechanism 13 moves downwards relative to the magnetic rod sleeve motion structure 12 to a magnetic absorption working position, the magnetic beads carrying the nucleic acid are adsorbed on the surface of the magnetic rod sleeve again, the magnetic rod sleeve motion structure 12 moves upwards to a waiting position, the carrying platform moving mechanism 21 moves to the next reaction hole position in the deep hole plate 3 to be aligned with the magnetic rod sleeve, and the actions are repeated until all steps of cracking, adsorption, washing and elution required by nucleic acid extraction reaction are completed.
After the reaction is completed, the magnetic rod movement mechanism 13 is kept at the magnetic attraction working position, the magnetic rod sleeve movement structure 12 and the platform deck movement mechanism 21 move cooperatively until the hole position where the original magnetic bead is located is aligned with the magnetic rod sleeve 4, the magnetic rod sleeve movement structure moves downwards relative to the magnetic rod sleeve movement structure 12 to the unloading position, the magnetic beads attached to the magnetic rod sleeve 4 and the magnetic rod sleeve are unloaded and fall into the deep hole plate, the mechanisms of the extraction and mixing device reset, and the platform deck movement mechanism 21 moves the deep hole plate which is loaded with purified nucleic acid after the reaction is completed to the operation working position to wait for the operator to take away the purified nucleic acid.
By this time, the whole nucleic acid extraction reaction is finished, and the operator can repeat the whole process to continuously extract the nucleic acid.
The above description is only exemplary of the present invention and should not be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. The utility model provides a rotatory stirring formula nucleic acid extraction element's extraction mixing device which characterized in that includes: a portal frame and an extraction and uniform mixing module;
the extraction and blending module is arranged on the portal frame and moves up and down on the portal frame so as to extract and blend the sample;
the extraction and uniform mixing module comprises a magnetic bar sleeve movement structure and a guide rail;
the magnetic rod sleeve moving structure is connected with the guide rail through the sliding block connecting units arranged at the two ends of the magnetic rod sleeve moving structure so as to realize ascending or descending on the guide rail.
2. The extraction and blending device of claim 1, wherein the guide rail is arranged in parallel on a gantry and comprises a movable slider unit;
the slider unit is used for being connected with the slider connecting unit so as to realize the sliding connection between the magnetic rod sleeve movement structure and the guide rail and further realize the ascending or descending of the magnetic rod sleeve movement structure.
3. The extraction and blending apparatus of claim 2,
the slide block connecting unit comprises a first slide block connecting unit and a second slide block connecting unit which are positioned at two ends of the magnetic rod sleeve movement structure;
the first sliding block connecting unit and the second sliding block connecting unit are respectively in sliding connection with a first sliding block and a second sliding block which are arranged on the guide rail.
4. The extraction blending device of claim 2, wherein the extraction blending module further comprises a magnetic bar movement structure;
the magnetic bar motion structure comprises a third slide block connecting unit and a fourth slide block connecting unit which are positioned at two ends of the magnetic bar motion structure;
the third slider connecting unit and the fourth slider connecting unit are respectively connected with the third slider and the fourth slider arranged on the guide rail in a sliding manner so as to realize the ascending or descending of the magnetic bar moving structure on the guide rail.
5. The extraction and blending device of claim 4, wherein the extraction and blending module further comprises a magnetic bar motion motor; the magnetic rod movement motor is used for connecting the magnetic rod sleeve movement structure and the magnetic rod movement structure and driving the magnetic rod sleeve movement structure and the magnetic rod movement structure to be relatively close to or far away from each other on the guide rail.
6. The extraction and blending device of claim 4, wherein the magnetic rod movement structure further comprises a magnetic rod sleeve lifting motor; the magnetic rod sleeve lifting motor is used for connecting the magnetic rod movement structure and the magnetic rod sleeve movement structure and driving the magnetic rod movement structure and the magnetic rod sleeve movement structure to synchronously ascend or descend on the guide rail.
7. The extraction and blending device of claim 1, wherein the magnetic rod sleeve movement structure further comprises a magnetic rod sleeve rotating motor; the magnetic rod sleeve rotating motor is installed on the magnetic rod sleeve moving structure and used for driving the magnetic rod sleeve to rotate so as to uniformly mix the sample and the magnetic bead mixture.
8. A rotary stirring type nucleic acid extraction device is characterized by comprising
Extracting a blending device and a deep hole plate moving device;
the blending device comprises a portal frame and an extraction blending module; the extraction and blending module is arranged on the portal frame and moves up and down on the portal frame so as to extract and blend the sample;
the deep hole plate moving device is provided with a deep hole plate and a deep hole plate limiting structure;
the deep hole plate is used for placing a sample to be detected, magnetic beads for reaction and a reagent;
the deep hole plate limiting structure is used for fixing the deep hole plate on the deep hole plate moving device.
9. The nucleic acid extraction apparatus according to claim 8, wherein the deep-well plate moving means further comprises a heating structure for heating the deep-well plate; the heating structure is arranged below the deep hole plate limiting structure so as to meet the temperature requirement during nucleic acid extraction reaction.
10. The nucleic acid extraction apparatus according to claim 8, wherein the deep-well plate moving device further comprises a stage moving structure and a deep-well plate stage structure;
the platform deck moving structure is provided with two parallel guide rails;
the deep hole plate carrying platform structure is arranged on the two guide rails and moves along the guide rails.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023313456.3U CN214830280U (en) | 2020-12-31 | 2020-12-31 | Rotary stirring formula nucleic acid extraction element |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023313456.3U CN214830280U (en) | 2020-12-31 | 2020-12-31 | Rotary stirring formula nucleic acid extraction element |
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| Publication Number | Publication Date |
|---|---|
| CN214830280U true CN214830280U (en) | 2021-11-23 |
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ID=78955642
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202023313456.3U Active CN214830280U (en) | 2020-12-31 | 2020-12-31 | Rotary stirring formula nucleic acid extraction element |
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| Country | Link |
|---|---|
| CN (1) | CN214830280U (en) |
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2020
- 2020-12-31 CN CN202023313456.3U patent/CN214830280U/en active Active
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