CN114214185A - Nucleic acid extraction instrument - Google Patents
Nucleic acid extraction instrument Download PDFInfo
- Publication number
- CN114214185A CN114214185A CN202111344041.9A CN202111344041A CN114214185A CN 114214185 A CN114214185 A CN 114214185A CN 202111344041 A CN202111344041 A CN 202111344041A CN 114214185 A CN114214185 A CN 114214185A
- Authority
- CN
- China
- Prior art keywords
- hole
- magnetic separation
- reagent
- magnetic
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 18
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 18
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 18
- 238000000605 extraction Methods 0.000 title claims abstract description 10
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 41
- 238000007885 magnetic separation Methods 0.000 claims abstract description 39
- 238000011534 incubation Methods 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 238000003753 real-time PCR Methods 0.000 claims abstract description 7
- 239000011324 bead Substances 0.000 claims description 14
- 238000001514 detection method Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000007599 discharging Methods 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Abstract
The invention belongs to the field of medical instruments, and particularly relates to a nucleic acid extractor. The utility model provides a nucleic acid extraction appearance, includes pipetting device, magnetic separation device, incubates the dish which characterized in that: the device comprises a base, a plurality of reagent clamping strips, a plurality of incubation discs, a control panel and a magnetic separation device, wherein the incubation discs are fixed on the base through slide rails; the liquid transfer device and the magnetic separation device are sequentially arranged side by side, and the incubation disc sequentially penetrates through the liquid transfer device and the magnetic separation device through the sliding rail; the reagent clamping strips are sequentially arranged in the incubation disc, and are longitudinally opposite to the liquid transfer device and the magnetic separation device; the reagent card strip comprises a sample placing hole, a disposable suction head hole, a disposable magnetic sleeve hole, a reagent hole, a magnetic separation hole and a fluorescent quantitative pcr tube placing hole. The invention can better simplify the separation detection process and has higher efficiency of nucleic acid extraction.
Description
Technical Field
The invention belongs to the field of medical instruments, and particularly relates to a nucleic acid extractor.
Background
Most of common nucleic acid extraction instruments in the market at present adopt a 96-pore plate to contain lysis solution, magnetic beads, cleaning solution 1, cleaning solution 2 and eluent. Reagent components corresponding to 8 x 2 groups of samples were placed on a 96-well plate. At least one 96-deep well plate is required to be placed in each experiment, and when the sample size is less than 16, reagents are wasted. In addition, a common nucleic acid extractor in the market at present needs a manual pipettor to accurately pipette 2-5uL of samples to magnetic beads or lysis solution, and after nucleic acid extraction is finished, the pipettor is used to move the extracted nucleic acid to a PCR tube for the next amplification operation. Requiring time for a significant amount of laboratory staff.
Disclosure of Invention
The invention aims to provide a nucleic acid extractor to solve the defects in the prior art.
The technical scheme adopted by the invention for solving the technical problem is as follows:
a nucleic acid extraction instrument comprises a liquid transfer device, a magnetic separation device, an incubation disc and a plurality of reagent card strips, wherein the reagent card strips are arranged in the incubation disc, the incubation disc is fixed on a base through a sliding rail, and one side of the base is provided with a control panel for controlling the liquid transfer device, the magnetic separation device and the incubation disc;
the liquid transfer device and the magnetic separation device are sequentially arranged side by side, and the incubation disc sequentially penetrates through the liquid transfer device and the magnetic separation device through the sliding rail;
the reagent clamping strips are sequentially arranged in the incubation disc, and are longitudinally opposite to the liquid transfer device and the magnetic separation device; the reagent card strip comprises a sample placing hole, a disposable suction head hole, a disposable magnetic sleeve hole, a reagent hole, a magnetic separation hole and a fluorescent quantitative pcr tube placing hole.
Further, move liquid device includes first portal frame, install calandria module and first lead screw motor and calandria linear electric motor on the first portal frame, first lead screw motor drive calandria module up-and-down motion, calandria linear electric motor drive calandria imbibition flowing back and move back the liquid head.
Furthermore, the magnetic separation device comprises a second portal frame, the magnetic bead suction module, a second lead screw motor and a magnetic separation motor are arranged on the second portal frame, the second lead screw motor drives the magnetic bead suction module to move up and down, and the magnetic separation motor drives the magnetic bead suction module to suck the magnetic beads.
Further, it includes a plurality of draw-in groove to hatch the dish, reagent card strip sets up between the draw-in groove, hatches dish and slide rail sliding connection, hatches the dish and still is connected with servo motor, the dish is hatched in the step motor drive along the motion of slide rail direction, it still is provided with on the dish to hatch and blocks the strip with the reagent the corresponding temperature control module in hole is placed including placing sample hole, disposable suction head hole, disposable magnetic sleeve hole, reagent hole, magnetic separation hole and fluorescence ration pcr pipe to the reagent card strip.
The invention has the advantages that:
the device is provided with a precise liquid-transfering discharging gun, a sample only needs to be roughly sucked to a sample hole position during an experiment, the sample is automatically and precisely transferred by the instrument, nucleic acid extraction operation is carried out, a product is automatically moved to a PCR tube integrated on a reagent card strip after extraction is finished, and an experimenter only needs to take down the tube and place the tube into a real-time fluorescence quantitative PCR for quantitative operation.
Drawings
FIG. 1 is a schematic representation of the present invention
FIG. 2 is a front view of a reagent card strip of the present invention.
Detailed Description
In order to make the technical means, the original characteristics, the achieved purposes and the effects of the invention easy to understand, the invention is further described with reference to the figures and the specific embodiments.
Example 1
As shown in fig. 1 and 2, a nucleic acid extracting apparatus comprises a liquid transferring device, a magnetic separation device, an incubation disc 8, and a plurality of reagent card strips 9, wherein the reagent card strips 9 are arranged in the incubation disc 8, the incubation disc 8 is fixed on a base through a slide rail, and a control panel 1 is arranged on one side of the base and used for controlling the liquid transferring device, the magnetic separation device and the incubation disc 8;
the liquid transfer device and the magnetic separation device are sequentially arranged side by side, and the incubation disc 8 sequentially penetrates through the liquid transfer device and the magnetic separation device through a sliding rail;
the reagent card strips 9 are sequentially arranged and arranged in the incubation disc 8, and the reagent card strips 9 are longitudinally opposite to the liquid transfer device and the magnetic separation device; the reagent card strip comprises a sample placing hole 91, a disposable suction head hole 92, a disposable magnetic sleeve hole 93, a reagent hole, a magnetic separation hole 98 and a fluorescent quantitative pcr tube placing hole 99. The sample hole 91 is used for placing a sample tube, the disposable pipette tip hole 92 is used for placing a pipette tip, the disposable magnetic sleeve hole 93 is used for placing a magnetic sleeve of a magnetic separation device, the reagent holes comprise a first reagent hole 94, a second reagent hole 95, a third reagent hole 96 and a fourth reagent hole 97, the reagent holes are used for storing reagents for the pipette device, the magnetic separation hole 98 is used for the worker magnetic separation device, and the fluorescent quantitative pcr tube placing hole 99 is used for storing an extracted product.
The liquid transfer device comprises a first portal frame, a rifle arranging module 7, a first lead screw motor 4 and a rifle arranging linear motor 5 are installed on the first portal frame, the rifle arranging module 7 is driven by the first lead screw motor 4 to move up and down, and the rifle arranging linear motor 5 is driven to arrange a liquid sucking and discharging head and a liquid returning and discharging head.
The magnetic separation device comprises a second portal frame, the magnetic bead suction module 6, a second lead screw motor 2 and a magnetic separation motor 3 are arranged on the second portal frame, the second lead screw motor drives the magnetic bead suction module 2 to move up and down, and the magnetic separation motor 3 drives the magnetic bead suction module to suck magnetic beads.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, and that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (4)
1. The utility model provides a nucleic acid extraction appearance, includes pipetting device, magnetic separation device, incubates the dish which characterized in that: the device comprises a base, a plurality of reagent clamping strips, a plurality of incubation discs, a control panel and a magnetic separation device, wherein the incubation discs are fixed on the base through slide rails;
the liquid transfer device and the magnetic separation device are sequentially arranged side by side, and the incubation disc sequentially penetrates through the liquid transfer device and the magnetic separation device through the sliding rail;
the reagent clamping strips are sequentially arranged in the incubation disc, and are longitudinally opposite to the liquid transfer device and the magnetic separation device; the reagent card strip comprises a sample placing hole, a disposable suction head hole, a disposable magnetic sleeve hole, a reagent hole, a magnetic separation hole and a fluorescent quantitative pcr tube placing hole.
2. The nucleic acid extractor of claim 1, wherein: the liquid transfer device comprises a first portal frame, a rifle arranging module, a first lead screw motor and a rifle arranging linear motor are mounted on the first portal frame, the first lead screw motor drives the rifle arranging module to move up and down, and the rifle arranging linear motor drives the rifle to suck and discharge liquid and retreat and move a liquid head.
3. The nucleic acid extractor of claim 1, wherein: the magnetic separation device comprises a second portal frame, a magnetic bead suction module, a second lead screw motor and a magnetic separation motor are arranged on the second portal frame, the second lead screw motor drives the magnetic bead suction module to move up and down, and the magnetic separation motor drives the magnetic bead suction module to suck magnetic beads.
4. The nucleic acid extractor according to claim 1, wherein the incubation disc includes a plurality of slots, the reagent card strip is disposed between the slots, the incubation disc is slidably connected to the slide rail, the incubation disc is further connected to a servo motor, the step motor drives the incubation disc to move along the slide rail, and a temperature control module corresponding to the reagent card strip including a sample placement hole, a disposable pipette tip hole, a disposable magnetic sleeve hole, a reagent hole, a magnetic separation hole, and a fluorescent quantitative pcr tube placement hole is further disposed on the incubation disc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111344041.9A CN114214185A (en) | 2021-11-14 | 2021-11-14 | Nucleic acid extraction instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111344041.9A CN114214185A (en) | 2021-11-14 | 2021-11-14 | Nucleic acid extraction instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114214185A true CN114214185A (en) | 2022-03-22 |
Family
ID=80697097
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111344041.9A Pending CN114214185A (en) | 2021-11-14 | 2021-11-14 | Nucleic acid extraction instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114214185A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115386457A (en) * | 2022-09-23 | 2022-11-25 | 广州湾区生物科技有限公司 | Nucleic acid extraction instrument |
-
2021
- 2021-11-14 CN CN202111344041.9A patent/CN114214185A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115386457A (en) * | 2022-09-23 | 2022-11-25 | 广州湾区生物科技有限公司 | Nucleic acid extraction instrument |
| CN115386457B (en) * | 2022-09-23 | 2023-11-28 | 广州湾区生物科技有限公司 | Nucleic acid extraction instrument |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108048302B (en) | Full-automatic nucleic acid extractor | |
| WO2022237181A1 (en) | Dna and rna nucleic acid co-extraction and detection system | |
| CN114214185A (en) | Nucleic acid extraction instrument | |
| CN110007103A (en) | A kind of compact independence multichannel liquor removing workstation | |
| CN114836309A (en) | Full-automatic nucleic acid detection and analysis equipment and system | |
| CN203855572U (en) | Instrument for extracting active substance of organisms | |
| CN108315243B (en) | Automatic sample adding system | |
| CN115155400B (en) | Full-automatic magnetic dispersion solid phase extraction device | |
| CN111197004A (en) | Full-automatic real-time fluorescence quantitative PCR workstation | |
| CN214553646U (en) | Absorption mixing device of multiple reagent of single passageway | |
| CN217709479U (en) | Full-automatic nucleic acid methylation treatment facility, analytical equipment and processing mechanism | |
| CN115353953A (en) | Square cabin for health examination | |
| CN214991615U (en) | DNA, RNA nucleic acid co-extraction and detecting system | |
| CN115178315A (en) | Full-automatic high-flux workstation integration device | |
| CN212077070U (en) | Draw liquid system and nucleic acid extraction device add | |
| CN113640535A (en) | Integrated form nucleic acid sample automatic processing equipment | |
| CN107629952B (en) | Nucleic acid hybridization instrument | |
| CN217709467U (en) | Full-automatic nucleic acid detection and analysis equipment and system | |
| CN216073836U (en) | High-flux nucleic acid extraction and purification device | |
| CN214458054U (en) | Sample extraction device and sample analysis system | |
| CN217103902U (en) | Full-automatic nucleic acid analysis and detection device | |
| CN115386457B (en) | Nucleic acid extraction instrument | |
| CN218637406U (en) | Multi-sample extraction device for nucleic acid extractor | |
| CN215162724U (en) | Nucleic acid extraction all-in-one machine | |
| CN219689719U (en) | Nucleic acid extraction and amplification integrated system and kit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20220322 |
|
| WD01 | Invention patent application deemed withdrawn after publication |