CN217709467U - Full-automatic nucleic acid detection and analysis equipment and system - Google Patents
Full-automatic nucleic acid detection and analysis equipment and system Download PDFInfo
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- CN217709467U CN217709467U CN202220862561.2U CN202220862561U CN217709467U CN 217709467 U CN217709467 U CN 217709467U CN 202220862561 U CN202220862561 U CN 202220862561U CN 217709467 U CN217709467 U CN 217709467U
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Abstract
The utility model discloses a full-automatic nucleic acid detection and analysis equipment and system, include: the kit comprises a kit placing rack, a liquid transferring device and a fluorescence detection device; the kit placing rack is provided with a sample area, a reagent area, a cracking heating area, a magnetic separation area and a constant temperature amplification reaction area and is used for placing kits with different components; the sample area is provided with a single hole site, and a single sample tube is placed in one-time nucleic acid detection to prevent cross contamination among samples. To full-automatic nucleic acid detection analytical equipment structure complicacy commonly used, it is bulky, introduce the pollution easily, heavy in weight, high price, to the detection that daily sample volume is few, there is the wasting of resources, the problem that nimble transition detection can't be accomplished, through setting up single detection sample volume into one, different reagent components of pre-distribution of different hole sites of finished product kit, use disposable pipettor transfer liquid, it is miniaturized to realize equipment structure, the simplification, the lightweight, and effectively prevented the pollution, a plurality of equipment can parallelly connected range increase the detection volume.
Description
Technical Field
The utility model belongs to the technical field of nucleic acid detection, especially, relate to a full-automatic nucleic acid detection analytical equipment.
Background
The nucleic acid detection is to extract and purify target DNA/RNA nucleic acid in a human body sample, amplify the target DNA/RNA nucleic acid along with time or temperature change by a biochemical method, and detect the quantity and concentration of the target DNA/RNA nucleic acid in the amplification process to judge the condition of the target nucleic acid quantity in the human body sample for diagnosing diseases.
Because the nucleic acid detection process needs to crack and release cells in a human body sample, and the amount of DNA/RNA nucleic acid is detected through the steps of extraction, purification, amplification, optical detection and the like, the common full-automatic nucleic acid detection and analysis equipment has the problems of complex structure, large volume, easy pollution introduction, heavy weight and high price, and the problems of resource waste and incapability of flexible transition detection exist for daily detection with small sample amount because the related methods of the steps are different and the sample loading amount is generally designed to be dozens or more in one time in order to improve the detection efficiency.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a full-automatic nucleic acid testing analytical equipment and system sets up the detection sample volume once to one, and different reagent components of pre-shipment are realized to different hole sites of finished product kit, realize that equipment structure is miniaturized, simplification, lightweight to effectively prevent the pollution, a plurality of equipment can parallelly connected range increase detection volume.
In order to solve the above problem, the technical scheme of the utility model is that:
a fully automated nucleic acid detection and analysis device comprising: the kit comprises a kit placing rack, a liquid transferring device and a fluorescence detection device;
the kit placing rack is provided with a sample area, a reagent area, a cracking heating area, a magnetic separation area and a constant temperature amplification reaction area and is used for placing kits with different components; the sample area is provided with a single hole site, and a single sample tube is placed in one-time nucleic acid detection to prevent cross contamination among samples;
the pipetting device finishes nucleic acid extraction through processes of sample adding, pipetting, mixing, magnetic separation and the like through preset program steps;
the fluorescence detection device is arranged below the constant-temperature amplification reaction zone, and when the pipetting device inputs the extracted nucleic acid into the kit of the constant-temperature amplification reaction zone for constant-temperature amplification reaction, the fluorescence data of the constant-temperature amplification reaction zone are read.
According to an embodiment of the present invention, the liquid transfer device includes a disposable liquid transfer device, a liquid transfer device mounting frame, a Z-direction mover, and a Y-direction mover;
the disposable pipettor is placed on the pipettor mounting frame before equipment runs, a piston of the disposable pipettor is clamped on the Z-direction shifter, and a cylinder of the disposable pipettor is fixed on the pipettor mounting frame;
the Z-direction shifter is used for driving the disposable pipettor to move up and down along a Z axis;
and the Y-direction mover is used for driving the liquid transfer device to move repeatedly along the Y axis.
According to the utility model discloses an embodiment, fluorescence detection device includes fluorescence data acquisition module, mobile module, fluorescence data acquisition module with the mobile module electricity is connected, the mobile module drive fluorescence data acquisition module is in the below in constant temperature amplification reaction zone comes and goes.
According to the utility model discloses an embodiment, fluorescence data acquisition module is multichannel fluorescence detector, realizes the detection of multiple wavelength fluorescence.
According to an embodiment of the present invention, the full-automatic nucleic acid detection and analysis device further comprises a support frame, wherein the support frame is divided into a nucleic acid extraction working area, a fluorescence detection working area and a control area;
the support frame is a semi-surrounding structure, the nucleic acid extraction working area is positioned in a surrounding ring of the support frame, the fluorescence detection working area is positioned below the support frame, and the control area is positioned on the back of the support frame.
The utility model provides a full-automatic nucleic acid testing analytic system, includes a plurality of the embodiment of the utility model provides an in full-automatic nucleic acid testing analytic device, realize the nucleic acid testing of many samples.
The utility model discloses owing to adopt above technical scheme, make it compare with prior art and have following advantage and positive effect:
1) The utility model relates to an embodiment's full-automatic nucleic acid testing analytical equipment, owing to adopt single sample, reduced a nucleic acid testing's sample size, make equipment volume and weight reduce by a wide margin, convenient removal and transportation are fit for the customer that the sample size is little.
2) The utility model discloses a full-automatic nucleic acid testing analytical equipment in the embodiment owing to adopt disposable pipettor, has avoided the aerosol pollution that traditional method cylinder pipettor used repeatedly formation.
3) The utility model discloses a full-automatic nucleic acid detection analytical equipment in embodiment owing to adopt single sample, use disposable pipettor, has effectively eliminated between the sample, the cross contamination of formation such as amplification product and residue.
4) The utility model discloses a full-automatic nucleic acid detecting and analyzing system in the embodiment adopts single use and many parallelly connected compound mode of using, can be suitable for the condition that the sample size fluctuates and changes, improves work efficiency and equipment availability factor, practices thrift the cost.
Drawings
FIG. 1 is a schematic view of the overall structure of a fully automatic nucleic acid detection and analysis apparatus according to an embodiment of the present invention;
fig. 2 is a schematic view of a structure of a liquid transfer device in an embodiment of the present invention;
FIGS. 3a to 3d are schematic views of a reagent rack and a reagent cartridge according to an embodiment of the present invention;
fig. 4 is a schematic structural diagram of a fluorescence detection device according to an embodiment of the present invention.
Description of reference numerals:
1: a support frame; 2: a pipetting device; 21: a Z-direction driving motor; 22: an injection drive motor; 23: a slider bracket; 24: a concave support; 25: an injection pump; 25a: a syringe pump cavity; 25b: a syringe pump piston; 25c: a suction head; 26a: a pipetting device mounting rack; 26b: a Y-direction driving motor; 3: a kit placing rack; 31a: a magnetic frame; 31b: heating the block at a constant temperature of 42 ℃;31c: cracking the heating block at 60 ℃;32: kit A; 33: kit B; 34: a sample tube; 4: a fluorescence detection device; 41: a fluorescence data acquisition module; 42a: a drive motor; 42b: a slide rail; 42c: a transmission belt.
Detailed Description
The following will further describe the fully automatic nucleic acid detecting and analyzing apparatus and system provided by the present invention in detail with reference to the accompanying drawings and specific embodiments. The advantages and features of the present invention will become more fully apparent from the following description and appended claims.
This embodiment is complicated to full-automatic nucleic acid detection analytical equipment structure commonly used, it is bulky, introduce the pollution easily, heavy weight, high price, to the detection that daily sample volume is few, there is the wasting of resources, the problem that nimble transition detection can't be accomplished, a full-automatic nucleic acid detection analytical equipment is provided, set up single detection sample volume into one, different reagent components of pre-distribution of different hole sites of finished product kit, use disposable pipettor to shift liquid, it is miniaturized to realize equipment structure, it is simple, light-weighted, and effectively prevented the pollution, a plurality of equipment can increase the detection volume by parallel arrangement.
The full-automatic nucleic acid detection and analysis equipment comprises: the kit comprises a kit placing rack, a liquid transferring device and a fluorescence detection device; the kit placing rack is provided with a sample area, a reagent area, a cracking heating area, a magnetic separation area and a constant temperature amplification reaction area and is used for placing kits with different components; a single hole site is arranged in the sample area, and a single sample tube is placed in one-time nucleic acid detection to prevent cross contamination among samples;
the pipetting device is internally provided with a preset program, and the nucleic acid extraction is completed according to the preset program. It should be noted that the built-in preset program is not an improvement of the present invention, and the preset program may be a control program in an existing full-automatic nucleic acid detection and analysis device.
The fluorescence detection device is arranged below the constant-temperature amplification reaction zone, and when the liquid transfer device inputs the extracted nucleic acid into the kit of the constant-temperature amplification reaction zone for constant-temperature amplification reaction, the fluorescence data of the constant-temperature amplification reaction zone is read.
Referring to fig. 1, the automatic nucleic acid detecting and analyzing apparatus includes a support frame 1, a pipetting device 2, a reagent kit holder 3, and a fluorescence detecting device 4. Wherein, support frame 1 carries out subregion setting and installation with each functional area in the equipment. The support frame is divided into a nucleic acid extraction working area, a fluorescence detection working area and a control area, and can be in a semi-enclosed structure. Wherein, the nucleic acid extraction working area is positioned in the enclosure of the support frame 1, the fluorescence detection working area is positioned below the support frame 1, and the control area is positioned at the back of the support frame 1. The nucleic acid extraction working area can be open, and the reagent kit, the disposable pipettor and the sample tube can be placed in the nucleic acid extraction working area by opening the operation door.
And the pipetting device 2 completes the mixing of the liquid in the kit through pipetting and pipetting, and finally transfers the liquid to an amplification reaction position of the kit placing frame 3 so as to carry out fluorescence detection.
The kit placing rack 3 is used for placing the sample tube and the kit and is provided with a 60 ℃ heating cracking position, a 40 ℃ constant temperature amplification position, a sample position, a reagent position and the like.
The fluorescence detection device 4 drives the fluorescence data acquisition module through the fluorescence transmission assembly, and reads fluorescence data at a constant temperature of 40 ℃.
Specifically, referring to fig. 2, the liquid transfer device 2 is provided with a syringe pump 25, which is a disposable syringe pump, i.e., a disposable pipette. The injection pump cavity 25a is clamped on the concave bracket 24 on the slide rail frame, the injection pump piston 25b is clamped on the slide block bracket 23 of the track, and the injection driving motor 22 drives the slide block bracket 23 to move up and down during liquid suction/transfusion, so that the injection pump piston 25b reciprocates to finish liquid suction and transfusion. The Z-direction driving motor 21 drives the whole injection pump 25 to descend to a set liquid sucking/transfusion position through the rotation of a screw rod, or after the whole injection pump is ascended to a distance that the end part of the suction head 25c has no interference with the reagent kit placing rack 3, the Y-direction driving motor 26B transmits and drives the liquid-moving device 2 to move in the Y direction, so that the injection pump 25 stays above the hole position of the reagent kit A box 32 or the reagent kit B box 33 which needs to be moved.
Referring to fig. 3a to 3d, the pipetting device 2 transfers the liquid in the sample tube 34 into the cartridge a case 32 or the cartridge B case 33 and mixes the liquid with the pre-stored liquid to form a reaction liquid. In order to ensure that the reaction liquid is uniformly mixed, the reaction liquid is repeatedly pumped by an injection pump.
Then the reaction liquid is input into the hole site reagent kit of the 60 ℃ cracking heating block 31c through the injection pump 25 for cracking reaction.
In order to extract nucleic acid in the reaction liquid, the reaction liquid after cleavage is sucked into the tip 25c by the syringe pump 25, and the magnetic beads are magnetically adsorbed in the wall of the syringe pump by moving the pipetting device 2 so that the tip 25c is held at the middle position of the magnetic rack 31a for 5 to 10 minutes. When the liquid is discharged by the syringe pump 25, the magnetic beads are separated from the tip 25c, and the nucleic acid in the reaction liquid adheres to the magnetic beads and is separated from the liquid, thereby completing the extraction of the nucleic acid.
Adding the nucleic acid amplification reaction liquid into a hole of a constant-temperature heating block 31b at 42 ℃ to perform constant-temperature amplification on the extracted nucleic acid; at the same time, the fluorescence data acquisition module 41 below the well site periodically reads the fluorescence data.
Referring to fig. 4, the fluorescence detection device 4 is installed below the reagent kit holder 3, and the fluorescence data acquisition module 41 drives the driving motor 42a to rotate forward and backward to rotate the driving belt 42c forward and backward, so as to drive the light reading hole of the fluorescence data acquisition module 41 installed on the sliding rail 42b to align with the hole site of the 42 ℃ constant temperature heating block 31b, and further read the fluorescence value of the liquid in the hole site reagent kit.
The fluorescence data acquisition module 41 can be designed as a multi-channel light reading hole to realize multi-wavelength fluorescence detection, and one sample can detect joint inspection of various diagnoses.
After the fluorescence data detection is finished, the reagent tube at the hole position of the 42 ℃ constant temperature heating block 31B corresponding to the kit B box 33 is blocked by a tube cover to prevent pollution.
Compared with the traditional nucleic acid extraction and detection equipment which adopts the inherent structures of batch samples and disposable suction heads and air cylinders, the full-automatic nucleic acid detection and analysis equipment in the embodiment has the advantage that cross contamination is easily formed by air cylinder liquid inlet and aerosol residue.
Furthermore, the utility model also provides a full-automatic nucleic acid detecting and analyzing system, including a plurality of above-mentioned full-automatic nucleic acid detecting and analyzing equipment, a plurality of full-automatic nucleic acid detecting and analyzing equipment also can make up and the split, realizes the detection of a plurality of samples, uses in a flexible way. And the single full-automatic nucleic acid detection and analysis equipment has light weight, easy movement and small volume and can be used in small spaces such as vehicles, mobile laboratories and the like.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments. Even if various changes are made to the present invention, the changes are still within the scope of the present invention if they fall within the scope of the claims and their equivalents.
Claims (6)
1. A fully automatic nucleic acid detection and analysis device, comprising: the kit comprises a kit placing rack, a liquid transferring device and a fluorescence detection device;
the kit placing rack is provided with a sample area, a reagent area, a cracking heating area, a magnetic separation area and a constant temperature amplification reaction area and is used for placing kits with different components; the sample area is provided with a single hole site, and a single sample tube is placed in one-time nucleic acid detection to prevent cross contamination among samples;
a preset program is arranged in the liquid transferring device, and nucleic acid extraction is completed according to the preset program;
the fluorescence detection device is arranged below the constant-temperature amplification reaction zone, and when the pipetting device inputs the extracted nucleic acid into the kit of the constant-temperature amplification reaction zone for constant-temperature amplification reaction, the fluorescence data of the constant-temperature amplification reaction zone are read.
2. The fully automatic nucleic acid detection and analysis apparatus according to claim 1, wherein the pipetting device comprises a disposable pipette, a pipette mounting rack, a Z-direction mover, a Y-direction mover;
the disposable pipettor is placed on the pipettor mounting frame before equipment runs, a piston of the disposable pipettor is clamped on the Z-direction shifter, and a cylinder of the disposable pipettor is fixed on the pipettor mounting frame;
the Z-direction shifter is used for driving the disposable pipettor to move up and down along a Z axis;
and the Y-direction mover is used for driving the liquid transfer device to move repeatedly along the Y axis.
3. The fully automatic nucleic acid detecting and analyzing apparatus according to claim 1, wherein the fluorescence detecting device comprises a fluorescence data collecting module and a moving module, the fluorescence data collecting module is electrically connected to the moving module, and the moving module drives the fluorescence data collecting module to move back and forth below the isothermal amplification reaction zone.
4. The fully automatic nucleic acid detecting and analyzing apparatus of claim 3, wherein the fluorescence data collecting module is a multi-channel fluorescence detector for detecting fluorescence with multiple wavelengths.
5. The fully automatic nucleic acid detection and analysis device according to claim 1, further comprising a support frame, wherein the support frame is divided into a nucleic acid extraction working area, a fluorescence detection working area and a control area;
the support frame is a semi-surrounding structure, the nucleic acid extraction working area is positioned in a surrounding ring of the support frame, the fluorescence detection working area is positioned below the support frame, and the control area is positioned on the back of the support frame.
6. A fully automatic nucleic acid detecting and analyzing system comprising a plurality of the fully automatic nucleic acid detecting and analyzing apparatuses according to any one of claims 1 to 5, for detecting nucleic acids in a plurality of samples.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220862561.2U CN217709467U (en) | 2022-04-15 | 2022-04-15 | Full-automatic nucleic acid detection and analysis equipment and system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220862561.2U CN217709467U (en) | 2022-04-15 | 2022-04-15 | Full-automatic nucleic acid detection and analysis equipment and system |
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| Publication Number | Publication Date |
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| CN217709467U true CN217709467U (en) | 2022-11-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202220862561.2U Active CN217709467U (en) | 2022-04-15 | 2022-04-15 | Full-automatic nucleic acid detection and analysis equipment and system |
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| CN (1) | CN217709467U (en) |
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