CN105277726A - Protein chip fully-automated high-throughput analysis method and protein chip fully-automated high-throughput analysis apparatus - Google Patents
Protein chip fully-automated high-throughput analysis method and protein chip fully-automated high-throughput analysis apparatus Download PDFInfo
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Abstract
The present invention discloses a protein chip fully-automated high-throughput analysis apparatus, which comprises a control host machine, a display electrically connected to the control host machine, and a fully-automatic protein chip workstation, wherein the table surface of the fully-automatic protein chip workstation is provided with a sample treatment, a reagent treatment system, a plate washing system and a signal acquisition system, a free mechanical arm and a liquid adding system are added above the table surface of the work station, and the sample treatment system comprises a suction head support frame, a sample support frame, a reagent support frame and a barcode scanner. With the protein chip analysis apparatus of the present invention, the simultaneous detection on the multiple samples, the standard substance and the quality control substance can be achieved, the calculation on the concentration of the current batch of the samples through the current batch of the standard substance and the quality control substance is achieved, and the detection result is accurate and stable; and the multiple samples share the one standard substance or quality control substance so as to achieve the agent saving effect.
Description
Technical field
The present invention relates to protein chip detection technique field, specifically disclose the full-automatic method for high-flux analysis of a kind of protein chip and device.
Background technology
Albumen, antibody chip are a kind of by solid-phase matrix (silicon chip, glass, nitrocellulose filter) surperficial highdensity ankyrin or antibody, have the effect that high flux detects multiple indexs such as antigen in biological specimen or antibody.Compared with the ELISA method detected with traditional single index, protein chip has advantages such as detecting flux is large, sample requirement is little, detection efficiency high (can simultaneously detect up to a hundred indexs in an aperture), therefore be particularly suitable for clinically to test item while multiple index, such as, detect while multiple tumor markers.But, because protein chip experimental implementation process is numerous and diverse, responsive to experiment condition, experiment between different batches, due to the fine distinction of experiment condition, can cause the difference of experimental result, and batch in test because experiment condition is almost completely the same, therefore there is good stability.
In order to the stability that improves experimental result with avoid contacting of the sample of operating personnel and potential band infective virus, someone proposes analysis process and the equipment of protein chip full-automation.But the full-automatic analysis process of current protein chip and equipment are all based on typical curve timing alignment, the independent tupe of single sample.This pattern has certain advantage, such as timing alignment typical curve, can save reagent; The independent tupe of single sample can be accomplished that sample arrives at any time, analyze at any time.But due to Parallel testing while that standard items, quality-control product and sample not being, therefore, use the sample of next batch of the typical curve calculating of one batch, testing result has larger deviation.
Summary of the invention
There is the defect of relatively large deviation in the present invention, and combines the feature of sample batch processing clinically in order to overcome the full-automatic analytical equipment of protein chip in prior art, provide a kind of protein chip full-automatic high throughput analysis device.Multiple sample and standard items, quality-control product can be put together and detect simultaneously by this analytical equipment, thus calculate the concentration of specimens of present lot with the standard items of present lot and quality-control product, make testing result more accurate; Meanwhile, multiple sample shares standard items and quality-control product and can reach the effect of saving reagent.
Another object of the present invention is to provide the full-automatic method for high-flux analysis of a kind of protein chip.
Object of the present invention is achieved by the following technical programs:
A kind of protein chip analytical equipment, comprise main control system, the display be electrically connected with main control system, full-automatic protein chip workstation, the table top of described full-automatic protein chip workstation is provided with: sample processing system, agent treated system, wash plate system, signal acquiring system, and the top of operation instrument table top is provided with free mechanical arm and liquid feeding system; Described sample processing system, agent treated system, wash plate system, signal acquiring system, free mechanical arm be electrically connected with main control system;
Described sample processing system comprises for the suction nozzle carrier placing suction nozzle pallet, for placing the sample carrier of sample tube, supplying to place reagent carrier and the barcode scanner of reagent needed for integrated enzyme reaction;
Suction nozzle carrier, sample carrier and reagent carrier arrange into a line on the table top of workstation, the side of the arranging line of suction nozzle carrier, sample carrier and reagent carrier is provided with guide rail, and barcode scanner scans the information of enrolling sample and reagent to sample and reagent at slide on rails;
Described free mechanical arm comprises robot support frame, captures motor and left and right mechanical finger, robot support frame is slided along overlying guide rail by drive unit, drive unit comprises belt wheel and elastic metal strip, belt wheel is rotated and mobile manipulator bracing frame, robot support frame is by capturing motor connection for transmission device, the leading screw of output shaft is driven to rotate and drive left and right mechanical finger, described left and right mechanical finger is tabular, built-in soft silica gel is held chi frame, and left and right mechanical finger is by the rotation adjustment distance of feed screw nut in bracing frame.
Preferably, described suction nozzle carrier, sample carrier, reagent carrier are from left to right arranged in order into line on the table top of workstation, and described barcode scanner is along guide rail successively scanning samples and reagent.More preferably, described suction nozzle carrier places 4 suction nozzle pallets, and each suction nozzle pallet can place 96 suction nozzles; Described sample carrier designs 10 altogether; Described reagent carrier arranges 2 altogether.
Preferably, described agent treated system comprises and hatches platform, thermostatically-controlled equipment and oscillation device; Hatch the right outside side that platform is positioned at last reagent carrier; Hatch platform and be provided with multiple chi frame; Thermostat is positioned at the below of hatching platform, and vibrating device is positioned at the below of thermostatically-controlled equipment.
As more preferably scheme, described in hatch platform and be provided with 4 chi frames, 4 chi frames by four independently chip form a chipset, a chipset can process 1 to 64 sample simultaneously.Most preferably, described chi frame is the rigid plastic frame of 12.7cmX8.5cmX1.2cm, has four pairs of tongues inside chi frame, for supporting and fixing 4 pieces of protein chips.
As a kind of preferred version, described liquid feeding system is 1 or 2 automatic sampling gun combinations.More preferably, described automatic sampling gun comprises bracing frame and feed head, and bracing frame connects motor and leading screw by bearing, and leading screw drives feed head as parts moving linearly, and described feed head internal piston bar is connected with driver part.
Preferably, wash described in plate system comprise tank for washing plate for washing plate, hold the washing lotion bucket of washing lotion, draw washing lotion and waste liquid wash wrench and provide wash plate power wash trigger.Wash the right inside survey that wrench is positioned at reagent carrier, tank for washing plate and wash trigger washing on the right side of wrench and be arranged in order; Described washing lotion bucket is positioned at hatches on the right side of platform.Chip is put in and washes in trigger fixed frame by free mechanical arm, and wash wrench and draw cleansing solution repeatedly rinse from washing plate bucket chip, the waste liquid drawn pumps into waste liquid tank in washing lotion bucket by washing wrench.Wash wrench can finely tune the diverse location in chip by computer instruction, with extinction waste liquid to greatest extent.
Described signal acquiring system comprises laser or chemiluminescence imaging device and gathers groove.Gathering groove is arranged on the table top of protein chip automation mechanized operation instrument, gathers groove and chip can be moved to immediately below laser or chemiluminescence imaging device; Gather groove when chip moves to by free mechanical arm and move to immediately below imaging device, laser or chemiluminescence imaging device start the information of acquisition chip and conversion imaging.Described laser or chemiluminescence imaging device comprise photomultiplier and utilizing emitted light assembly, and utilizing emitted light assembly comprises the optical filtering of filtration 700 to 800 wavelength.Signal acquiring system can adopt fluorescent exciting or chemiluminescence detection optical path signal, and fluorescence can adopt the optical filtering of 532nm, and chemiluminescence can adopt the optical filtering of 425nm.
Described data handling system comprises digital quantizer, light intensity detector.Wherein light intensity detector is connected to the normalized that computing machine carries out signal intensity and background.The Excel data processing software H16GastricSoftware that described data handling system comprises the editor of inventor's exploitation carries out abnormal data analyze and control automatically, greatly improves the shortcoming that conventional bio-chip needs manual suppressing exception background signal.
Utilize a method for protein chip analytical equipment analyzing proteins chip described above, comprise the steps:
S1. chip, reagent, sample are put into respectively the relevant position of protein chip analytical equipment, barcode scanner scans the bar code on reagent and sample, different reagent and sample is added up by different barcode formats, and automatically loading the information of reagent and sample, robotization generates detection scheme and Report mode;
S2. after detection scheme generates, main control system drives liquid feeding system, first required dilution is added to formulation test tube by liquid feeding system, and automatically a certain amount of sample or standard items or quality-control product are joined in corresponding test tube, blow and beat mixing to dilute simultaneously, finally the liquid after dilution is added in respective chip automatically and carries out first step enzyme linked immunoassay;
S3. after first step enzyme linked immunoassay terminates, chip is transferred to wash in plate system and is carried out washing plate by automatic mechanical arm, completing to be transferred to by chip after washing plate hatches on platform, liquid feeding system adds corresponding mark and detects antibody, carry out second time enzyme linked immunoassay, after second time enzyme linked immunoassay terminates, chip is transferred to wash in plate system and is carried out washing plate by automatic mechanical arm again, completing to be transferred to by chip after washing plate hatches on platform again, add developer after hatching, chip moves to be washed plate system and carries out washing plate, completes after washing plate, terminates enzyme linked immunoassay;
S4. after enzyme linked immunoassay terminates, chip is transferred to the collection imaging of carrying out signal in signal acquiring system by free mechanical arm, image analysis software is analyzed image automatically, extract the intensity of signaling point and bales catch except the intensity of background signal, data analysis software is analyzed the signal intensity extracted, automatic rejection abnormal signal, automatically data normalization is carried out, curve fitting software carries out the matching of typical curve automatically, and calculate the concrete concentration detecting each Testing index in sample, the sample of the comprehensive typing of report preparing system, the information of chip and testing result, provide examining report.
Compared with prior art, the present invention has following beneficial effect:
1. the sample processing system that formed by ad hoc structure of the present invention, multiple sample and standard items, quality-control product can be realized to put together and detect simultaneously, thus by the standard items of present lot and the concentration of specimens of quality-control product calculating present lot, make testing result more accurate; Meanwhile, multiple sample shares standard items and quality-control product and can reach the effect of saving reagent.
2. protein chip analytical equipment of the present invention, distributed by science and sample processing system, agent treated system are set, wash plate system, signal acquiring system, can realize adopting a set of technique, to the full-automatic operation that protein chip detects, comprise from Sample Dilution, to report generation all by instrumentation.
3. the infosystem of protein chip analytical equipment of the present invention and clinical LIMS system docking, directly can load the information detecting sample, can also check testing result simultaneously by LIMS system from clinical LIMS system.
4. protein chip analytical equipment of the present invention is suitable for for fluorescence detecting system and chemiluminescence detection system simultaneously, is suitable for the quantitative detecting analysis of protein chip or qualitative detection analysis simultaneously; Membrane DNA chip or slide chip are suitable for simultaneously.
5. protein chip analytical equipment of the present invention increases a large amount of mistake error corrections in interpretation of result system, adds the reliability of testing result.The described probe washing trigger adjusts position by computer, achieves multiple spot and washes plate.
6. the present invention is tabular by arranging mechanical finger, built-in soft silica gel can be held chi frame, left and right mechanical finger, by the rotation adjustment distance of feed screw nut in bracing frame, improves the stability of grabbing device, avoids the risk that midway protein chip framework drops.
figure of description
Fig. 1. the table top layout of protein chip analytical equipment.
Fig. 2. the complete machine view of protein chip analytical equipment.
Illustrate: 1. suction nozzle carrier; 2. suction nozzle pallet; 3. barcode scanner; 4. arrive level detecting apparatus; 5. sample carrier; 6. reagent carrier; 7. chip; 8. chi frame; 9. hatch platform; 10. run-bake-needle fault position; 11. washing lotion buckets; 12. washing lotion bucket carriers; 13. wash trigger; 14. tank for washing plate; 15. wash wrench; 16. gather groove; 17. automatic sampling guns; 18. mechanical operation arms; 19 laser or chemiluminescence imaging device.
Fig. 3. the software control flow chart of protein chip analytical equipment.
Fig. 4. the principle of work process flow diagram of protein chip analytical equipment.
The scanned picture of Fig. 5 .humanTh1/Th2QuantibodyArray protein chip.
Embodiment
Be further explained the present invention below in conjunction with specific embodiment, accompanying drawing, only for exemplary illustration, can not be interpreted as the restriction to this patent; In order to better the present embodiment is described, some parts of accompanying drawing have omission, zoom in or out, and do not represent the size of actual product; To those skilled in the art, in accompanying drawing, some known features and explanation thereof may be omitted is understandable.Describe position relationship in accompanying drawing only for exemplary illustration, the restriction to this patent can not be interpreted as.
embodiment 1
As shown in Figure 1 and 2, the invention provides a kind of protein chip analytical equipment, comprise main control system, the display be electrically connected with main control system and full-automatic protein chip workstation.The table top of described full-automatic protein chip workstation is provided with: sample processing system, agent treated system, wash plate system and signal acquiring system; The top of full-automatic protein chip tiered workstation rotunda is provided with free mechanical arm and liquid feeding system, described sample processing system, agent treated system, wash plate system, signal acquiring system, free mechanical arm and liquid feeding system and control main frame electrical connection.
Wherein, described sample processing system comprises for the suction nozzle carrier 1 placing suction nozzle pallet 2, for placing the sample carrier 5 of sample, supplying to place reagent carrier 6 and the barcode scanner 3 of reagent needed for integrated enzyme reaction.Suction nozzle carrier 1, sample carrier 5 and reagent carrier 6 arrange into a line on the table top of operation instrument, suction nozzle carrier 1, the side of the arranging line of sample carrier 5 and reagent carrier 6 is provided with guide rail, barcode scanner 3 is at slide on rails, sample and reagent are scanned, read the bar code on sample and reagent, and the information of this sample and reagent is loaded in analytic system automatically, for interpretation of result and report generation, in addition, barcode scanner 3 front is also provided with level detecting apparatus 4, barcode scanner 3 can be allowed accurately to find corresponding sample or reagent strip shape code position to level detecting apparatus 4,
Preferably, described suction nozzle carrier 1, sample carrier 5, reagent carrier 6 are from left to right arranged in order into line on the table top of workstation, and described barcode scanner 3 is along guide rail successively scanning samples and reagent.More preferably, described suction nozzle carrier 1 places 4 suction nozzle pallets 2, and each suction nozzle pallet 2 can place 96 suction nozzles; Described sample carrier 5 designs 10 altogether; Described reagent carrier 6 arranges 2 altogether.
Described free mechanical arm 18 is arranged on the top of full-automatic protein chip tiered workstation rotunda, under the control of main control system, mechanical operation arm 18 can move by multiple degrees of freedom above full-automatic protein chip tiered workstation rotunda, realizes chip to move at sample processing system, agent treated system, washes between plate system and signal acquiring system and move around.
Described free mechanical arm 18 comprises robot support frame, captures motor and left and right mechanical finger, robot support frame is slided along overlying guide rail by drive unit, drive unit comprises belt wheel and elastic metal strip, belt wheel is rotated and mobile manipulator bracing frame, robot support frame is by capturing motor connection for transmission device, the leading screw of output shaft is driven to rotate and drive left and right mechanical finger, described left and right mechanical finger is tabular, built-in soft silica gel is held chi frame, and left and right mechanical finger is by the rotation adjustment distance of feed screw nut in bracing frame.
Described liquid feeding system is one group of automatic sampling gun 17, can be that 1 or 2 automatic sampling guns 17 combine.The quantitative reagent be placed on reagent carrier 6, under the control of main control system, can add in sample and dilute sample or standard items are carried out to gradient dilution or sample and reagent added on chip 7 to carry out enzyme linked immunoassay by automatic sampling gun 17.Described automatic sampling gun 17 comprises bracing frame and feed head, and bracing frame connects motor and leading screw by bearing, and leading screw drives feed head as parts moving linearly, and described feed head internal piston bar is connected with driver part.
In addition, the table top of described full-automatic protein chip workstation is also provided with run-bake-needle fault position 10, and run-bake-needle fault position 10 is positions that automatic sampling gun 17 destroys suction nozzle.
Described agent treated system forms by hatching platform 9, thermostatically-controlled equipment and oscillation device.Hatch the right outside side that platform 9 is positioned at reagent carrier 6, hatch platform 9 and be provided with chi frame 8, be preferably 4 chi frames, 4 chi frames by four independently chip form a chipset, a chipset can process 1 to 64 sample simultaneously.More preferably, described chi frame 8 is the rigid plastic frame of 12.7cmX8.5cmX1.2cm, and inner side has four pairs of tongues, for supporting and fixing the protein chip that 4 pieces is matrix with standard slide or membrane DNA chip; Thermostat is one piece of thermal conductive metal plate, be positioned at the below of hatching platform, enzyme is exempted from course of reaction, and thermostat can heat hatching platform 9, and by hatching platform 9 by the chip be delivered on it of homogeneous temperature, thus by the temperature constant of chip at a design temperature.Vibrating device is positioned at the below of thermostatically-controlled equipment, can drive thermostatically-controlled equipment and on chip vibration, oscillation intensity is controlled by main control system.
Wash plate system comprise tank for washing plate 14 for washing plate, hold the washing lotion bucket 11 of washing lotion, draw washing lotion and expense liquid wash wrench 15, provide wash plate power wash trigger 13.Washing lotion bucket 11 is positioned on washing lotion bucket carrier 12, washes the right inside survey that wrench 15 is positioned at reagent carrier 6, tank for washing plate 14 and wash trigger 13 washing on the right side of wrench 15 and be arranged in order; Described washing lotion bucket 11 is positioned at hatches on the right side of platform 9.Under the influence of air pressure, wash wrench 15 and be injected in chip by the washing lotion in washing lotion bucket 11, washing trigger 13 provides power that the chip in tank for washing plate 14 is completed to wash plate, after washing the bundle that hardens, washes wrench 15 and is blotted by waste liquid, repeat operation, to reach the effect of washing plate.
Described signal acquiring system comprises laser or chemiluminescence imaging device 19 and gathers groove 16.Gathering groove 16 is arranged on the table top of protein chip automation mechanized operation instrument, preferably, gathers groove 16 and is positioned at the right side washing trigger 13; And laser or chemiluminescence imaging device 19 are arranged on directly over collection groove 16; Gather groove 16 when chip is fixed to by free mechanical arm 17 and moves to immediately below imaging device 19, gathering laser above groove 16 or chemiluminescence imaging device and start the information of acquisition chip and conversion imaging in addition record.Wherein, described laser or chemiluminescence imaging device 19, comprise photomultiplier and utilizing emitted light assembly, utilizing emitted light assembly comprises the optical filtering of filtration 700 to 800 wavelength.Signal acquiring system can adopt fluorescent exciting or chemiluminescence detection optical path signal, and fluorescence can adopt the optical filtering of 532nm, and chemiluminescence can adopt the optical filtering of 425nm.
Image analysis software is analyzed image automatically, extract the intensity of signaling point and bales catch except the intensity of background signal; Data analysis software is analyzed the signal intensity extracted, and automatic rejection abnormal signal, carries out data normalization automatically.Curve fitting software carries out the matching of typical curve automatically, and calculates the concrete concentration detecting each Testing index in sample.The information of the sample/chip of the comprehensive typing of report preparing system and testing result, provide examining report.When data analysis software carries out automated analysis to the chip signal gathered, first, Analysis quality control product signal: undesirable, termination analysis declaration test failure; Meet the requirements, carry out standard curve fit, and calculate the detected value detecting each index in sample, and detected value is loaded in examining report; Finally provide as above examining report.Whole process, instrument full-automation is carried out.
Described data handling system comprises digital quantizer, light intensity detector.Wherein light intensity detector is connected to the normalized that computing machine carries out signal intensity and background.The Excel data processing software H16GastricSoftware that described data handling system comprises the editor of inventor's exploitation carries out abnormal data analyze and control automatically, greatly improves the shortcoming that conventional bio-chip needs manual suppressing exception background signal.
The software control flow process of the present embodiment is as shown in Figure 3:
Comprise (1) and start step;
(2) sample, chip, reagent information mass are automatically imported process;
(3) robotization of detection scheme generates;
(4) sample, quality-control product, standard items mass, robotization reaction controlling;
(5) sample, quality-control product, standard items mass, automation signal collection and conversion;
(6) quality-control product analysis, judges Quality Control result;
(7) standard curve fit;
(8) testing result calculating, interpretation and report generation.
The principle of work of the protein chip analyzer described in the present embodiment as shown in Figure 4, completes the control to action between each function system of the present embodiment by electric control system and control software design.
embodiment 2
Below use the embodiment that described in embodiment 1, the quantitative chip of the humanTh1/Th2 of protein chip automatic partition analysis apparatus to Rui Boao operates.First, added on reagent carrier 6 corresponding in analytical equipment by reagent components corresponding in kit, barcode scanner 3 scans the bar code of reagent automatically.Be placed on by HumanTh1/Th2QuantibodyArray protein chip and hatch on platform 9 in agent treated system, be placed on by serum sample on the sample carrier 5 of analytical equipment, barcode scanner 3 scans the bar code of sample automatically.Clicking operation interface starts to test button.Namely monitoring experiment operation is started.Whole experimentation is without the need to manual intervention.Test findings is provided with the form of examining report list.
When experiment starts, main control system from data can automatically load the information of respective chip, and operation scheme, use for experimental implementation, data analysis, report generation, simultaneously, bar code on barcode scanner 3 autoscan serum sample, and the information of sample is automatically loaded from clinical LIMS system, use for report generation.After test report generates, quantitative dilution can add in sample or standard items or quality-control product by automatic liquid-feeding system, and blows and beats mixing and dilute, and is added to by liquid in chip more afterwards to carry out first step enzyme and exempt from reaction.Enzyme is exempted from reaction and is carried out in agent treated system.Hatching through certain hour, chip is transferred to wash in plate system and is carried out washing plate by mechanical operation arm 17, is transferred in agent treated system by chip again later.Liquid feeding system adds corresponding mark and detects antibody, carries out secondary enzyme and exempts from reaction, adds developer after carrying out washing plate reaction later again.After enzyme linked immunoassay terminates, chip is transferred to the collection imaging of carrying out chemiluminescence signal in chemiluminescence imaging device by mechanical operation arm 17, the scanned picture of humanTh1/Th2QuantibodyArray protein chip as shown in Figure 5, in Fig. 5, the chip QA12456321 of first row is standard items, the chip QA12456322 of second row is for detecting sample, standard items operate with detection sample synchronization, and employing works as the secondary concentration detecting sample when time typical curve calculates, and makes testing result more accurate.Image analysis software is analyzed image automatically, extract the intensity of signaling point and bales catch except the intensity of background signal, data analysis software is analyzed the signal intensity extracted, and automatic rejection abnormal signal, carries out data normalization automatically.Curve fitting software carries out the matching of typical curve automatically, and calculates the concrete concentration detecting each Testing index in sample.The sample of the comprehensive typing of report preparing system, the information of chip and testing result, provide examining report.
Claims (10)
1. a protein chip analytical equipment, comprise main control system, the display be electrically connected with main control system, full-automatic protein chip workstation, it is characterized in that, the table top of described full-automatic protein chip workstation is provided with: sample processing system, agent treated system, wash plate system, signal acquiring system, and the top of operation instrument table top is provided with free mechanical arm and liquid feeding system; Described sample processing system, agent treated system, wash plate system, signal acquiring system, free mechanical arm be electrically connected with main control system;
Described sample processing system comprises for the suction nozzle carrier placing suction nozzle pallet, for placing the sample carrier of sample tube, supplying to place reagent carrier and the barcode scanner of reagent needed for integrated enzyme reaction;
Suction nozzle carrier, sample carrier and reagent carrier arrange into a line on the table top of workstation, the side of the arranging line of suction nozzle carrier, sample carrier and reagent carrier is provided with guide rail, and barcode scanner scans the information of enrolling sample and reagent to sample and reagent at slide on rails;
Described free mechanical arm comprises robot support frame, captures motor and left and right mechanical finger, robot support frame is slided along overlying guide rail by drive unit, drive unit comprises belt wheel and elastic metal strip, belt wheel is rotated and mobile manipulator bracing frame, robot support frame is by capturing motor connection for transmission device, the leading screw of output shaft is driven to rotate and drive left and right mechanical finger, described left and right mechanical finger is tabular, built-in soft silica gel is held chi frame, and left and right mechanical finger is by the rotation adjustment distance of feed screw nut in bracing frame.
2. protein chip analytical equipment according to claim 1, it is characterized in that, described suction nozzle carrier, sample carrier, reagent carrier are from left to right arranged in order into line on the table top of workstation, and described barcode scanner is along guide rail successively scanning samples and reagent.
3. protein chip analytical equipment according to claim 1, is characterized in that, described agent treated system comprises hatches platform, thermostatically-controlled equipment and oscillation device; Hatch the right outside side that platform is positioned at reagent carrier, hatch platform and be provided with multiple chi frame; Thermostat is positioned at the below of hatching platform, and vibrating device is positioned at the below of thermostatically-controlled equipment.
4. protein chip analytical equipment according to claim 3, is characterized in that, described in hatch platform and be provided with 4 chi frames, 4 chi frames by four independently chip form a chipset, a chipset can process 1 to 64 sample simultaneously.
5. protein chip analytical equipment according to claim 4, is characterized in that, described chi frame is the rigid plastic frame of 12.7cmX8.5cmX1.2cm, has four pairs of tongues inside chi frame, for supporting and fixing 4 pieces of protein chips.
6. protein chip analytical equipment according to claim 1, is characterized in that, described liquid feeding system is 1 or 2 automatic sampling gun combinations.
7. protein chip analytical equipment according to claim 6, it is characterized in that, described automatic sampling gun comprises bracing frame and feed head, and bracing frame connects motor and leading screw by bearing, leading screw drives feed head as parts moving linearly, and described feed head internal piston bar is connected with driver part.
8. protein chip analytical equipment according to claim 1, is characterized in that, described in wash plate system comprise tank for washing plate for washing plate, hold the washing lotion bucket of washing lotion, draw washing lotion and waste liquid wash wrench and provide wash plate power wash trigger; Wash the right inside survey that wrench is positioned at reagent carrier, tank for washing plate and wash trigger washing on the right side of wrench and be arranged in order; Described washing lotion bucket is positioned at hatches on the right side of platform.
9. protein chip analytical equipment according to claim 8, is characterized in that, described washes wrench drops into row washing lotion at the random angle of protein chip micropore absorption by computer fine setting.
10. utilize a method for protein chip analytical equipment analyzing proteins chip described in any one of claim 1 to 9, it is characterized in that, comprise the steps:
S1. chip, reagent, sample are put into respectively the relevant position of protein chip analytical equipment, barcode scanner scans the bar code on reagent and sample, different reagent and sample is added up by different barcode formats, and automatically loading the information of reagent and sample, robotization generates detection scheme and Report mode;
S2. after detection scheme generates, main control system drives liquid feeding system, first required dilution is added to formulation test tube by liquid feeding system, and automatically a certain amount of sample or standard items or quality-control product are joined in corresponding test tube, blow and beat mixing to dilute simultaneously, finally the liquid after dilution is added in respective chip automatically and carries out first step enzyme linked immunoassay;
S3. after first step enzyme linked immunoassay terminates, chip is transferred to wash in plate system and is carried out washing plate by automatic mechanical arm, completing to be transferred to by chip after washing plate hatches on platform, liquid feeding system adds corresponding mark and detects antibody, carry out second time enzyme linked immunoassay, after second time enzyme linked immunoassay terminates, chip is transferred to wash in plate system and is carried out washing plate by automatic mechanical arm again, completing to be transferred to by chip after washing plate hatches on platform again, add developer after hatching, chip moves to be washed plate system and carries out washing plate, completes after washing plate, terminates enzyme linked immunoassay;
S4. after enzyme linked immunoassay terminates, chip is transferred to the collection imaging of carrying out signal in signal acquiring system by free mechanical arm, image analysis software is analyzed image automatically, extract the intensity of signaling point and bales catch except the intensity of background signal, data analysis software is analyzed the signal intensity extracted, automatic rejection abnormal signal, automatically data normalization is carried out, curve fitting software carries out the matching of typical curve automatically, and calculate the concrete concentration detecting each Testing index in sample, the sample of the comprehensive typing of report preparing system, the information of chip and testing result, provide examining report.
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| CN108593939A (en) * | 2018-07-18 | 2018-09-28 | 广州瑞博奥生物科技有限公司 | A kind of full-automatic protein chip and its application |
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