CN105277726B - A kind of protein chip full-automation method for high-flux analysis and device - Google Patents
A kind of protein chip full-automation method for high-flux analysis and device Download PDFInfo
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- CN105277726B CN105277726B CN201410357880.8A CN201410357880A CN105277726B CN 105277726 B CN105277726 B CN 105277726B CN 201410357880 A CN201410357880 A CN 201410357880A CN 105277726 B CN105277726 B CN 105277726B
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Abstract
The invention discloses a kind of protein chip full-automation high throughput analysis device, including control main frame, the display that is electrically connected with control main frame and full-automatic protein chip work station.The table top of wherein described full-automatic protein chip work station is provided with sample processing system, agent treatment system, board-washing system, signal acquiring system, and the top of tiered workstation rotunda is provided with free mechanical arm and liquid feeding system.The sample processing system includes suction nozzle carrier, sample carrier, reagent carrier and barcode scanner.Protein chip analytical equipment of the present invention can be realized multiple samples and standard items, quality-control product while detected, and realize the standard items of present lot and quality-control product calculates the concentration of specimens of present lot so that testing result is more accurate, more stable；Meanwhile the shared standard items of multiple samples or quality-control product can reach the effect for saving reagent.
Description
Technical field
The present invention relates to protein chip detection technique field, specifically discloses a kind of protein chip full-automation high flux point
Analyse method and device.
Background technology
Albumen, antibody chip are one kind by solid-phase matrix（Silicon chip, glass, nitrocellulose filter）Surface is highdensity
Ankyrin or antibody, have the function that high flux detects multiple indexs such as antigen or antibody in biological specimen.With it is traditional
The ELISA method of single index detection is compared, and protein chip is with detection flux is big, sample requirement is small, detection efficiency is high（At one
Individual indexs up to a hundred can be detected in aperture simultaneously）The advantages that, therefore be particularly suitable for clinically to being detected while multiple indexs
Project, such as, detect while multiple tumor markerses.However, because protein chip experimental implementation process is numerous and diverse, to testing bar
Part is sensitive, the experiment between different batches, due to the fine distinction of experiment condition, you can cause the difference of experimental result, and criticize
Secondary interior experiment has preferable stability because experiment condition is almost completely the same.
In order to improve the stability of experimental result and avoid connecing for operating personnel and the potentially sample with infective virus
Touch, it has therefore already been proposed that the full-automatic analysis process of protein chip and equipment.However, current protein chip is full-automatic
Analysis process and equipment are all based on the independent tupe of standard curve timing alignment, single sample.The pattern has certain
Advantage, such as timing alignment standard curve, can save reagent；The independent tupe of single sample can accomplish sample at any time
To, analyze at any time.But because standard items, quality-control product and sample are not while Parallel testing, therefore, use batch
Standard curve calculates the sample of next batch, and testing result has larger deviation.
The content of the invention
The present invention in order to overcome in the prior art protein chip full-automation analytical equipment the defects of relatively large deviation be present, and
With reference to the characteristics of clinically sample batch processing, there is provided a kind of protein chip full-automation high throughput analysis device.The analysis fills
Put and multiple samples and standard items, quality-control product can be put together while detected, so as to use the standard items and quality-control product of present lot
Calculate the concentration of specimens of present lot so that testing result is more accurate；Meanwhile multiple samples share a standard items and quality-control product
The effect for saving reagent can be reached.
It is a further object to provide a kind of protein chip full-automation method for high-flux analysis.
The purpose of the present invention is achieved by the following technical programs：
A kind of protein chip analytical equipment, including control main frame, the display electrically connected with control main frame, full-automatic albumen
Chip operation station, the table top of the full-automatic protein chip work station are provided with：Sample processing system, agent treatment system, wash
Plate system, signal acquiring system, the top of operation instrument table top are provided with free mechanical arm and liquid feeding system；Described sample process
System, agent treatment system, board-washing system, signal acquiring system, free mechanical arm electrically connect with control main frame；
The sample processing system include for place suction nozzle pallet suction nozzle carrier, for place sample tube sample carrier,
Reagent carrier and barcode scanner for placing reagent needed for integrated enzyme reaction;
Suction nozzle carrier, sample carrier and reagent carrier arrange into a line, suction nozzle carrier, sample on the table top of work station
The side of the arranging line of carrier and reagent carrier is provided with guide rail, and barcode scanner is slided on guide rail to sample and reagent progress
Scan to enroll the information of sample and reagent；
The free mechanical arm includes robot support frame, crawl motor and left and right mechanical finger, robot support frame
Slided by drive device along overlying guide rail, drive device includes belt wheel and elastic metal strip, belt wheel is rotated simultaneously mechanically moving
Hand support frame, robot support frame drive the leading screw of output shaft to rotate and drive left and right by capturing motor connection transmission device
Mechanical finger, the left and right mechanical finger be tabular, and built-in soft silica gel holds chi frame, and left and right mechanical finger passes through branch
The rotation adjustment distance of feed screw nut in support.
Preferably, the suction nozzle carrier, sample carrier, reagent carrier on the table top of work station from a left side to
The right side is arranged in order into line, and the barcode scanner scans sample and reagent successively along guide rail.It is highly preferred that the suction nozzle carries
4 suction nozzle pallets are placed on frame, each suction nozzle pallet can place 96 suction nozzles；The sample carrier designs 10 altogether；It is described
Reagent carrier sets 2 altogether.
Preferably, the agent treatment system includes being incubated platform, thermostatically-controlled equipment and oscillation device；Incubate
Educate the right outside that platform is located at last reagent carrier；It is incubated platform and is provided with multiple chi frames；Thermostat is positioned at incubation platform
Lower section, vibrating device is located at the lower section of thermostatically-controlled equipment.
As more preferably scheme, the incubation platform is provided with 4 chi frames, and 4 chi frames are by four independent cores
Piece forms a chipset, and a chipset can handle 1 to 64 sample simultaneously.Most preferably, the chi frame is
12.7cm X 8.5cm X 1.2cm rigid plastic frame, chi frame inner side has four pairs of tongues, for supporting and fixing 4
Block protein chip.
As a kind of preferred scheme, the liquid feeding system is 1 or 2 automatic sampling gun combinations.It is highly preferred that it is described from
Dynamic sample loading gun includes support frame and feed head, and support frame connects motor and leading screw by bearing, and leading screw is as parts moving linearly
Feed head is driven, the feed head internal piston bar is connected with driving part.
Preferably, the board-washing system includes the tank for washing plate for board-washing, the washing lotion bucket for holding washing lotion, draws washing lotion and give up
The board-washing head of liquid and the board-washing machine that board-washing power is provided.Board-washing head, which is located in the right side of reagent carrier, to be surveyed, and tank for washing plate and board-washing machine exist
It is arranged in order on the right side of board-washing head；The washing lotion bucket position is on the right side of incubation platform.Chip is put in board-washing machine and fixed by free mechanical arm
In framework, board-washing head draws cleaning solution from board-washing bucket and chip is rinsed repeatedly, and the waste liquid drawn is pumped into washing lotion by board-washing head
Waste liquid tank in bucket.Board-washing head can be indicated to be finely adjusted the diverse location in chip by computer, with extinction waste liquid to greatest extent.
The signal acquiring system includes laser or chemiluminescence imaging device and collection groove.Collection groove is arranged on albumen core
On the table top of piece automation mechanized operation instrument, chip can be moved to immediately below laser or chemiluminescence imaging device by collection groove；When certainly
Chip is moved to collection groove by mechanical arm and is moved to immediately below imaging device, laser or chemiluminescence imaging device start
The information and conversion imaging of acquisition chip.Laser or the chemiluminescence imaging device includes photomultiplier and transmitting light group
Part, transmitting optical assembly include the optical filtering of 700 to 800 wavelength of filtration.Signal acquiring system can use fluorescent exciting or chemistry
Luminous light path signal, fluorescence can use 532 nm optical filtering, and chemiluminescence can adopt 425 nm optical filtering.
The data handling system includes digital quantizer, light intensity detector.Wherein light intensity detector is connected to meter
Calculation machine carries out the normalized of signal intensity and background.Described data handling system includes the editor's of inventor's exploitation
Excel data processing software H16 Gastric Software carry out abnormal data analysis and excluded automatically, improve biography significantly
The shortcomings that biochip of uniting needs manual suppressing exception background signal.
A kind of method using protein chip analytical equipment analyzing proteins chip as described above, comprise the following steps：
S1., chip, reagent, sample are respectively put into the relevant position of protein chip analytical equipment, barcode scanner pair
Bar code on reagent and sample is scanned, and counts different reagents and sample by different barcode formats, and add automatically
Carry the information of reagent and sample, automation generation detection scheme and Report mode；
S2. after detection scheme generation, required dilution is added to by control main frame driving liquid feeding system, liquid feeding system first
Formulate test tube, and a certain amount of sample or standard items or quality-control product be added in corresponding test tube automatically, at the same blow and beat mix into
Row dilution, finally the liquid after dilution is automatically added to carry out first step enzyme linked immunoassay in respective chip；
S3. after first step enzyme linked immunoassay terminates, chip is transferred in board-washing system and washed by automatic mechanical arm
Plate, chip is transferred on incubation platform after completing board-washing, liquid feeding system adds corresponding mark detection antibody, second of enzyme of progress
Join immune response, after second of enzyme linked immunoassay terminates, chip is transferred again into board-washing system by automatic mechanical arm
Row board-washing, chip is transferred on incubation platform again after completing board-washing, adds developer after incubation, chip is moved to board-washing system
Board-washing is carried out, after completing board-washing, terminates enzyme linked immunoassay；
S4. after enzyme linked immunoassay terminates, chip is transferred in signal acquiring system and carries out signal by free mechanical arm
Collection and imaging, image analysis software image is analyzed automatically, extract the intensity and automatic background correction letter of signaling point
Number intensity, DAS analyzed the signal intensity of extraction, automatic rejection abnormal signal, and the automatic data that carry out are returned
One changes, and curve fitting software carries out the fitting of standard curve automatically, and calculates the specific dense of each Testing index in detection sample
Degree, the sample of report preparing system synthesis typing, the information and testing result of chip, provides examining report.
Compared with prior art, the present invention has the advantages that：
1. the sample processing system that the present invention is made up of specific structure, it is possible to achieve by multiple samples and standard items, matter
Control product are put together while detected, and so as to calculate the concentration of specimens of present lot with the standard items of present lot and quality-control product, are made
It is more accurate to obtain testing result；Meanwhile the shared standard items of multiple samples and quality-control product can reach the effect for saving reagent.
2. protein chip analytical equipment of the present invention, sample processing system, agent treatment are allocated and set up by science
System, board-washing system, signal acquiring system, it is possible to achieve a set of technique is used, the full-automatic operation detected to protein chip,
Including from Sample Dilution, to report generation all by instrumentation.
, can be directly from facing 3. the information system of protein chip analytical equipment of the present invention and clinical LIMS system dockings
The information of loading detection sample in bed LIMS systems, while testing result can also be checked by LIMS systems.
4. protein chip analytical equipment of the present invention is fitted simultaneously for fluorescence detecting system and chemiluminescence detection system
With simultaneously quantitative detecting analysis or qualitative detection analysis to protein chip are applicable；Fitted simultaneously for membrane DNA chip or slide chip
With.
5. protein chip analytical equipment of the present invention increases substantial amounts of mistake error correction in interpretation of result system, increase
The reliability of testing result is added.The probe of the board-washing machine realizes multiple spot board-washing by computer adjustment position.
6. the present invention is tabular by setting mechanical finger, built-in soft silica gel can hold chi frame, left and right manipulator
Distance can be adjusted by the rotation of feed screw nut in support frame by referring to, and improved the stability of grabbing device, avoided midway protein chip
The risk that framework drops.
Figure of description
The table top layout of Fig. 1 protein chip analytical equipments.
The complete machine view of Fig. 2 protein chip analytical equipments.
Illustrate：1. suction nozzle carrier；2. suction nozzle pallet；3. barcode scanner；4. arrive level detecting apparatus；5. sample carries
Frame；6. reagent carrier；7. chip；8. chi frame；9. it is incubated platform；10. run-bake-needle fault position；11. washing lotion bucket；12. washing lotion bucket carrier；
13. board-washing machine；14. tank for washing plate；15. board-washing head；16. gather groove；17. automatic sampling gun；18. mechanical operation arm；19 laser or
Chemiluminescence imaging device.
The software control flow chart of Fig. 3 protein chip analytical equipments.
The operation principle flow chart of Fig. 4 protein chip analytical equipments.
The scanned picture of Fig. 5 .human Th1/Th2 Quantibody Array protein chips.
Embodiment
The present invention is further explained with reference to specific embodiment, being given for example only property of accompanying drawing explanation, no
It is understood that as the limitation to this patent；In order to more preferably illustrate the present embodiment, some parts of accompanying drawing have omission, zoomed in or out,
The size of actual product is not represented；To those skilled in the art, some known features and its explanation may in accompanying drawing
Omission will be understood by.Being given for example only property of position relationship explanation described in accompanying drawing, it is impossible to be interpreted as the limitation to this patent.
Embodiment 1
As shown in Figure 1 and 2, the invention provides a kind of protein chip analytical equipment, including control main frame and control main frame
The display of electrical connection and full-automatic protein chip work station.The table top of the full-automatic protein chip work station is provided with：
Sample processing system, agent treatment system, board-washing system and signal acquiring system；Automatically protein chip tiered workstation rotunda is upper
Side is provided with free mechanical arm and liquid feeding system, and described sample processing system, agent treatment system, board-washing system, signal are adopted
Collecting system, free mechanical arm and liquid feeding system electrically connect with control main frame.
Wherein, described sample processing system includes suction nozzle carrier 1, the sample for placing sample for placing suction nozzle pallet 2
Product carrier 5, reagent carrier 6 and barcode scanner 3 for placing reagent needed for integrated enzyme reaction.Suction nozzle carrier 1, sample carrier 5
Into a line, the arrangement of suction nozzle carrier 1, sample carrier 5 and reagent carrier 6 is arranged on the table top of operation instrument with reagent carrier 6
The side of line is provided with guide rail, and barcode scanner 3 slides on guide rail, sample and reagent are scanned, and reads sample and examination
Bar code in agent, and the information of the sample and reagent is automatically loaded into analysis system, for interpretation of result and report
Generation, in addition, the front of barcode scanner 3 is additionally provided with level detecting apparatus 4, barcode scanner 3 can be allowed to level detecting apparatus 4
It is accurate to find corresponding sample or reagent barcode position；
Preferably, the suction nozzle carrier 1, sample carrier 5, reagent carrier 6 on the table top of work station from left to right successively
Line is arranged in, the barcode scanner 3 scans sample and reagent successively along guide rail.It is highly preferred that on the suction nozzle carrier 1
4 suction nozzle pallets 2 are placed, each suction nozzle pallet 2 can place 96 suction nozzles；The sample carrier 5 designs 10 altogether；The examination
Agent carrier 6 sets 2 altogether.
The free mechanical arm 18 is arranged on the top of full-automatic protein chip tiered workstation rotunda, in the control of control main frame
Under system, mechanical operation arm 18 can be realized chip in the top multiple degrees of freedom movement of full-automatic protein chip tiered workstation rotunda
Move back and forth between sample processing system movement, agent treatment system, board-washing system and signal acquiring system.
The free mechanical arm 18 includes robot support frame, crawl motor and left and right mechanical finger, robot support
Frame is slided by drive device along overlying guide rail, and drive device includes belt wheel and elastic metal strip, belt wheel is rotated simultaneously moving machine
Tool hand support frame, robot support frame drive the leading screw of output shaft to rotate and drive a left side by capturing motor connection transmission device
Right mechanical finger, the left and right mechanical finger be tabular, and built-in soft silica gel holds chi frame, and left and right mechanical finger passes through
The rotation adjustment distance of feed screw nut in support frame.
Described liquid feeding system, it is one group of automatic sampling gun 17, can is that 1 or 2 automatic sampling guns 17 combine.Automatically
For sample loading gun 17 under the control of control main frame, it is right in sample to be added to the quantitative reagent being placed on reagent carrier 6
Sample be diluted or to standard items carry out gradient dilution or by sample and reagent be added on chip 7 carry out enzyme linked immunological it is anti-
Should.The automatic sampling gun 17 includes support frame and feed head, and support frame connects motor and leading screw by bearing, and leading screw is as straight
Line moving component drives feed head, and the feed head internal piston bar is connected with driving part.
In addition, being additionally provided with run-bake-needle fault position 10 on the table top of the full-automatic protein chip work station, run-bake-needle fault position 10 is automatic adds
Sample rifle 17 destroys the position of suction nozzle.
Described agent treatment system is made up of incubation platform 9, thermostatically-controlled equipment and oscillation device.Platform 9 is incubated positioned at examination
The right outside of agent carrier 6, it is incubated platform 9 and is provided with chi frame 8, preferably 4 chi frames, 4 chi frames are only by four
Vertical chip forms a chipset, and a chipset can handle 1 to 64 sample simultaneously.It is highly preferred that described chip frame
Frame 8 is 12.7cm X 8.5cm X 1.2cm rigid plastic frame, and inner side has four pairs of tongues, for supporting and fixing 4 pieces
Protein chip using standard slide or membrane DNA chip as matrix；Thermostat is one piece of thermal conductive metal plate, positioned at be incubated platform lower section,
Enzyme is exempted from course of reaction, and thermostat can heat to being incubated platform 9, and be uniformly delivered to temperature by being incubated platform 9
Chip thereon, so as to which the temperature of chip is constant in a design temperature.Vibrating device is located at the lower section of thermostatically-controlled equipment,
Thermostatically-controlled equipment and chip vibration thereon can be driven, oscillation intensity controls by control main frame.
Board-washing system includes the tank for washing plate 14 for board-washing, the washing lotion bucket 11 for holding washing lotion, draws washing lotion and takes washing for liquid
Wrench 15, the board-washing machine 13 that board-washing power is provided.Washing lotion bucket 11 is located on washing lotion bucket carrier 12, and board-washing first 15 is located at reagent carrier
Surveyed in 6 right side, tank for washing plate 14 and board-washing machine 13 are arranged in order on first 15 right side of board-washing；The washing lotion bucket 11 is right positioned at platform 9 is incubated
Side.Under the influence of air pressure, the washing lotion in washing lotion bucket 11 is injected into chip by board-washing first 15, and board-washing machine 13, which provides power, to be made to wash
Chip in board slot 14 completes board-washing, and after board-washing terminates, board-washing first 15 blots waste liquid, repeats operation, to reach board-washing
Effect.
The signal acquiring system includes laser or chemiluminescence imaging device 19 and collection groove 16.Collection groove 16 is arranged on
On the table top of protein chip automation mechanized operation instrument, it is preferable that collection groove 16 is located at the right side of board-washing machine 13；And laser or chemistry hair
Photoimaging equipment 19 is arranged on the surface of collection groove 16；When chip is fixed to collection groove 16 and movement by free mechanical arm 17
To immediately below imaging device 19, the laser or chemiluminescence imaging device of the top of collection groove 16 start the information of acquisition chip and turned
Change into as and recorded.Wherein, laser or the chemiluminescence imaging device 19, including photomultiplier and transmitting light group
Part, transmitting optical assembly include the optical filtering of 700 to 800 wavelength of filtration.Signal acquiring system can use fluorescent exciting or chemistry
Luminous light path signal, fluorescence can use 532 nm optical filtering, and chemiluminescence can adopt 425 nm optical filtering.
Image analysis software is analyzed image automatically, and intensity and the bales catch for extracting signaling point are strong except background signal
Degree；DAS is analyzed the signal intensity of extraction, automatic rejection abnormal signal, carries out data normalization automatically.
Curve fitting software carries out the fitting of standard curve automatically, and calculates the specific concentration of each Testing index in detection sample.Report
The information and testing result of sample/chip of generation system synthesis typing are accused, provides examining report.DAS is to adopting
When the chip signal of collection carries out automated analysis, first, Analysis quality control product signal：It is undesirable, termination analysis declaration experiment
Failure；Meet the requirements, carry out standard curve fit, and calculate the detected value of each index in detection sample, and detected value is added
It is downloaded in examining report；Finally provide as above examining report.Whole process, instrument is full-automatic to be carried out.
The data handling system includes digital quantizer, light intensity detector.Wherein light intensity detector is connected to meter
Calculation machine carries out the normalized of signal intensity and background.Described data handling system includes the editor's of inventor's exploitation
Excel data processing software H16 Gastric Software carry out abnormal data analysis and excluded automatically, improve biography significantly
The shortcomings that biochip of uniting needs manual suppressing exception background signal.
The software control flow of the present embodiment is as shown in Figure 3：
Start step including (1);
(2) sample, chip, reagent information mass are automatically imported process；
(3) the automation generation of detection scheme；
(4) sample, quality-control product, standard article batch, automation reaction controlling；
(5) sample, quality-control product, standard article batch, automation signal collection and conversion；
(6) quality-control product is analyzed, and judges Quality Control result；
(7) standard curve fit；
(8) testing result calculating, interpretation and report generation.
The operation principle of protein chip analyzer described in the present embodiment is as shown in figure 4, pass through electric control system and control
Software processed completes the control to being acted between each function system of the present embodiment.
Embodiment 2
It is that Rui Boao humanTh1/Th2 is determined with protein chip automated analysis device described in embodiment 1 below
The embodiment that amount chip is operated.First, it is right in analytical equipment corresponding reagent components in kit to be added to
On the reagent carrier 6 answered, barcode scanner 3 is scanned to the bar code of reagent automatically.By Human Th1/Th2
Quantibody Array protein chips are placed on the incubation platform 9 in agent treatment system, and serum sample is placed on into analysis
On the sample carrier 5 of device, barcode scanner 3 is scanned to the bar code of sample automatically.Clicking operation starts on interface
Test button.Start to monitor experimental implementation.Whole experiment process is without manual intervention.Result of the test is with the shape of examining report list
Formula is provided.
When experiment starts, control main frame from data can in automatic loading respective chip information, and operation scheme, for
Experimental implementation, data analysis, report generation use, meanwhile, the bar code on the automatically scanning serum sample of barcode scanner 3,
And load the information of sample automatically from clinical LIMS systems, so that report generation uses.After test report generation, automatically
Quantitative dilution can be added in sample or standard items or quality-control product by liquid feeding system, and blow and beat mixing and be diluted, it
Liquid is added into progress first step enzyme in chip again afterwards to exempt to react.Enzyme is exempted from reaction and carried out in agent treatment system.By certain
Chip is transferred in board-washing system and carries out board-washing by the incubation of time, mechanical operation arm 17, and chip is transferred into reagent again later
In processing system.Liquid feeding system adds corresponding mark detection antibody, carries out secondary enzyme and exempts to react, carries out board-washing again later
Developer is added after reaction.After enzyme linked immunoassay terminates, chip is transferred to chemiluminescence imaging device by mechanical operation arm 17
The interior collection for carrying out chemiluminescence signal and imaging, the scanning figure of human Th1/Th2 Quantibody Array protein chips
For piece as shown in figure 5, the chip QA12456321 of first row is standard items in Fig. 5, the chip QA12456322 of second row is detection
Sample, standard items and detection sample synchronization operation, calculated using when time standard curve when time concentration of detection sample so that detection
As a result it is more accurate.Image analysis software is analyzed image automatically, extract signaling point intensity and bales catch remove background signal
Intensity, DAS analyzed the signal intensity of extraction, automatic rejection abnormal signal, automatic to carry out data normalizing
Change.Curve fitting software carries out the fitting of standard curve automatically, and calculates the specific concentration of each Testing index in detection sample.
The sample of report preparing system synthesis typing, the information and testing result of chip, provide examining report.
Claims (8)
1. a kind of protein chip analytical equipment, including control main frame, the display electrically connected with control main frame, full-automatic albumen core
Piece work station, it is characterised in that the table top of the full-automatic protein chip work station is provided with：At sample processing system, reagent
Reason system, board-washing system, signal acquiring system, the top of operation instrument table top are provided with free mechanical arm and liquid feeding system；It is described
Sample processing system, agent treatment system, board-washing system, signal acquiring system, free mechanical arm and control main frame be electrically connected
Connect；
The sample processing system includes the suction nozzle carrier for placement suction nozzle pallet, the sample carrier for placing sample tube, for putting
Put the reagent carrier and barcode scanner of reagent needed for integrated enzyme reaction；
The suction nozzle carrier, sample carrier, reagent carrier are from left to right arranged in order into line on the table top of work station, and suction nozzle carries
The side of the arranging line of frame, sample carrier and reagent carrier is provided with guide rail, barcode scanner slided on guide rail to sample and
Reagent is scanned to enroll the information of sample and reagent；
The board-washing system includes the tank for washing plate for board-washing, the washing lotion bucket for holding washing lotion, the board-washing head for drawing washing lotion and waste liquid
With the board-washing machine for providing board-washing power；Board-washing head, which is located in the right side of reagent carrier, to be surveyed, and board-washing head is indicated in chip by computer
Diverse location is finely adjusted；Tank for washing plate and board-washing machine are arranged in order on the right side of board-washing head；The washing lotion bucket position is on the right side of incubation platform；
The free mechanical arm includes robot support frame, crawl motor and left and right mechanical finger, robot support frame and passed through
Drive device slides along overlying guide rail, and drive device includes belt wheel and elastic metal strip, belt wheel is rotated simultaneously mobile manipulator branch
Support, robot support frame drive the leading screw of output shaft to rotate and drive left and right machinery by capturing motor connection transmission device
Finger, the left and right mechanical finger be tabular, and built-in soft silica gel holds chi frame, and left and right mechanical finger passes through support frame
The rotation adjustment distance of interior feed screw nut.
2. protein chip analytical equipment according to claim 1, it is characterised in that the agent treatment system includes being incubated
Platform, thermostatically-controlled equipment and oscillation device；The right outside that platform is located at reagent carrier is incubated, platform is incubated and is provided with multiple chip frames
Frame；Thermostatically-controlled equipment is located at the lower section of thermostatically-controlled equipment positioned at the lower section for being incubated platform, vibrating device.
3. protein chip analytical equipment according to claim 2, it is characterised in that the incubation platform is provided with 4 chips
Four independent chips are formed a chipset by framework, 4 chi frames, and a chipset can handle 1 to 64 sample simultaneously
This.
4. protein chip analytical equipment according to claim 3, it is characterised in that the chi frame is 12.7cm X
8.5cm X 1.2cm rigid plastic frame, chi frame inner side has four pairs of tongues, for supporting and fixing 4 pieces of albumen cores
Piece.
5. protein chip analytical equipment according to claim 1, it is characterised in that the liquid feeding system be 1 or 2 from
Dynamic sample loading gun combination.
6. protein chip analytical equipment according to claim 5, it is characterised in that the automatic sampling gun includes support frame
With feed head, support frame connects motor and leading screw by bearing, and leading screw drives feed head, the sample-adding as parts moving linearly
Head internal piston bar is connected with driving part.
7. protein chip analytical equipment according to claim 1, it is characterised in that described board-washing head is finely tuned by computer
The absorption of row washing lotion is dropped into the random angle of protein chip micropore.
8. a kind of method using any one of claim 1 to the 7 protein chip analytical equipment analyzing proteins chip, its feature
It is, comprises the following steps：
S1., chip, reagent, sample are respectively put into the relevant position of protein chip analytical equipment, barcode scanner is to reagent
And the bar code on sample is scanned, different reagents and sample, and loading examination automatically are counted by different barcode formats
Agent and the information of sample, automation generation detection scheme and Report mode；
S2. after detection scheme generation, required dilution is added to formulation by control main frame driving liquid feeding system, liquid feeding system first
Test tube, and a certain amount of sample or standard items or quality-control product are added in corresponding test tube automatically, while it is dilute to blow and beat mixing progress
Release, be finally automatically added to the liquid after dilution to carry out first step enzyme linked immunoassay in respective chip；
S3. after first step enzyme linked immunoassay terminates, chip is transferred in board-washing system and carries out board-washing by automatic mechanical arm, complete
Chip is transferred to after into board-washing and is incubated on platform, the corresponding mark detection antibody of liquid feeding system addition, carries out enzyme-linked for the second time exempt from
Epidemic disease is reacted, and after second of enzyme linked immunoassay terminates, chip is transferred again into board-washing system and washed by automatic mechanical arm
Plate, chip is transferred on incubation platform again after completing board-washing, adds developer after incubation, chip is moved to the progress of board-washing system
Board-washing, after completing board-washing, terminate enzyme linked immunoassay；
S4. after enzyme linked immunoassay terminates, chip is transferred in signal acquiring system and carries out adopting for signal by free mechanical arm
Collect and be imaged, image analysis software is analyzed image automatically, extract signaling point intensity and bales catch except background signal
Intensity, DAS are analyzed the signal intensity of extraction, automatic rejection abnormal signal, automatic to carry out data normalizing
To change, curve fitting software carries out the fitting of standard curve automatically, and calculates the specific concentration of each Testing index in detection sample,
The sample of report preparing system synthesis typing, the information and testing result of chip, provide examining report.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410357880.8A CN105277726B (en) | 2014-07-25 | 2014-07-25 | A kind of protein chip full-automation method for high-flux analysis and device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410357880.8A CN105277726B (en) | 2014-07-25 | 2014-07-25 | A kind of protein chip full-automation method for high-flux analysis and device |
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| CN105277726B true CN105277726B (en) | 2018-02-02 |
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