CN201524578U - Reaction tray - Google Patents
Reaction tray Download PDFInfo
- Publication number
- CN201524578U CN201524578U CN2009202059305U CN200920205930U CN201524578U CN 201524578 U CN201524578 U CN 201524578U CN 2009202059305 U CN2009202059305 U CN 2009202059305U CN 200920205930 U CN200920205930 U CN 200920205930U CN 201524578 U CN201524578 U CN 201524578U
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- reaction
- reaction tray
- tray
- reaction cup
- cup
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Abstract
The utility model discloses a reaction tray which pertains to the technical field of parts of bio-analytic instruments. The reaction tray comprises a center shaft connecting hole and a bracket used for supporting a reaction cup, wherein the center shaft connecting hole and the bracket are integrally formed; and the reaction tray is detachably and firmly connected with the center shaft and is connected on a driving motor by the center shaft. As for the reaction tray, a mechanical inverse insertion prevention device is arranged for the bracket and/or the reaction cup, thus being capable of effectively preventing the reaction cup and/or a biological chip from being wrongly inserted or inserted toward different directions, improving the working efficiency of the whole biochip analytic instrument, and well maintaining the identity of reaction conditions of multiple chips. A heater is arranged at the middle lower part of the reaction cup, and is controlled by a constant temperature control module, thus being capable of guaranteeing balanced reaction temperature of the reaction cup.
Description
Technical field
The utility model belongs to bio-analysis instrument parts technical field, especially relates to the reaction tray that can use with the biochip analysis instrument device.
Background technology
Biochip be meant several square centimeters of sizes solid phase supporting dielectrics such as silicon chip, glass, gel or nylon membrane on, automatically print or light guiding chemical synthesising technology by robot, high-density biology molecule (as oligonucleotides, genetic fragment, cDNA fragment or polypeptide, the protein) microarray of making, hybridize with the sample molecule of mark then, by detect intensity of hybridization signal and distribute to the sequence and the quantity of target molecule carry out accurately, fast, a technology of identification, screening and the analysis of large information capacity.Be widely used at present in biomedical and the life science.The development of biochip technology will be served human health better for the achievement of basic research platform will be provided, and quick, inexpensive multiple proteins detects and analytical applications is achieved in tumour and other medical diagnosis on disease.
Biochip technology is had relatively high expectations to operating personnel at present, needs special technical staff to go to set up and operation.Because biochip is to place highdensity biomolecule microarry in small carrier, as micro-analysis, adopts manual operations, testing conditions is inconsistent very big to result's influence during use.In operating process, also need strictness to prevent the pollution of outer bound pair biochip and the cross pollution between the different biochip.The full automatic biochip analysis instrument of research and development high accuracy, high reliability and high efficiency makes vast clinical examination personnel or experimental analysis personnel break away from traditional detection mode, to be biochip technology future development, move towards a clinical important developing direction.
The biochip enterprise and the relevant industries in the whole world (comprising China) are being faced with unprecedented opportunities and challenge, nearly all internationally recognizable Biochip company all enlarges own product line as much as possible, to cater to the different demands that more clients propose.In the period of 10, U.S. government and industrial circle drop into 2,000,000,000 dollars of research and development and industrialization that are used for biochip nearly altogether in the past.Nearly all in the world transnational drugmaker all drops into huge fund, utilizes biochip to carry out the ultra-high throughput screening and the researchs such as pharmacogenetics, pharmacology genomics of new drug.Many famous IT, electric enterprise, for example Motorola, IBM, Hitachi etc. also identify oneself with the research and development ranks of bio chip device.
And as the visual plant of biochip detecting and analysing, the full-automatic biological chips analyzer that detects at combined tumor markers remains the technological difficulties that remain to be broken through multinomial.Biochip is being carried out in the analyte detection process, and chip tends to occur upset or towards inconsistent situation, and disposable multisample detected also has great limitation.Therefore, the reaction tray of its specialized application and analyzing biochips detection is particularly crucial for whole testing process.
The utility model content
The purpose of this utility model is to provide a kind of biochip that can effectively prevent upset to occur or towards inconsistent reaction tray, solve the defective that prior art exists.
For achieving the above object, the utility model adopts following technical scheme:
A kind of reaction tray comprises the axis connecting hole, some carriages that are used to support reaction cup, and described axis connecting hole and carriage are formed in one, and described reaction tray and axis detachably are fastenedly connected, and are connected to drive motors by axis.
Preferred scheme is: also comprise the reaction cup heater and the bracket plate that are used for the reaction cup heating, described reaction cup heater is arranged at a little less than the reaction cup middle part with upper/lower positions, and described bracket plate and carriage detachably are fastenedly connected.
More preferred scheme is: also comprise deceleration device, described deceleration device is arranged between drive motors and the heater, and this deceleration device is electrically connected with drive motors.
More preferred scheme is: described deceleration device is the harmonic wave speed reducing machine assembly.
More preferred scheme is: described carriage is provided with 45～90 reaction cup fixed bits, evenly equidistantly distributes.
More preferred scheme is: also comprise the chip position detecting sensor, described sensor probe is arranged at reaction cup fixed bit middle part or approaches bottom position, and this chip position detecting sensor is fixed under the heater.
More preferred scheme is: described carriage and/or reaction cup are provided with mechanical counnter attack plug device.
More preferred scheme is: described axis connecting hole is following concave curved surface to the middle part of carriage.
More preferred scheme is: described reaction tray and axis detachably are fastenedly connected by screw thread.
More preferred scheme is: described reaction tray is for circular.
Biochip as the testing medium is a rectangle glass substrate solid phase carrier, chip surface be divided into different discrete test zones (discrete test regions, DTR).Use the millimicro distribution technique, make sample be deposited in biochip surface with accurate predefine coordinate, each DTR zone covers different antibody.
The immunology principle that product utilization antigen combines with its corresponding antibodies specificity, on solid-phase matrix in conjunction with the monoclonal antibody of kinds of tumors mark, catch the corresponding tumor markers in examinee's serum, and, tumor markers is quantitatively detected according to light signal strength by specific chemical reaction generation optical signal.Biochip once can detect carcinomebryonic antigen (cEA), alpha-fetoprotein 16～20 kinds of different tumor markerses such as (AFP) simultaneously.
Tumor markers detects and adopts sandwich method (sandwich assay), add quantitative dilution (its effect is to make detected sample can evenly be spread in chip surface), add blood serum sample to be detected then, incubation 10min～2h, thing to be checked in the sample is combined with specific antibody among the chip surface DTR, after washing 3～5 times, add quantitative enzyme mark and detect antibody reagent (conjugation reagent, use horseradish peroxidase (HRP) mark), incubation 10min～2h, the thing combination to be checked that makes the enzyme mark detect antibody and combine with the DTR specific antibody, form antibody one antigen one antibody complex, the washing several times add quantitative signal reagent, produce fluorescence signal.Use the CCD camera, computer is handled the optical signalling of gathering, and is converted into actual numerical value at last, prints testing result.Thereby the specificity and the multi-parameter that realize biomarker are measured simultaneously.
The utility model compared with prior art has following advantage and beneficial effect:
Reaction tray described in the utility model, carriage and/or reaction cup are provided with mechanical counnter attack plug device, can effectively prevent reaction cup and/biochip misplug occurs or towards inconsistent situation, improve the operating efficiency of whole biochip analysis instrument, and well kept the homogeneity of the reaction condition of multicore sheet.Position, middle and lower part in reaction cup is provided with heater, this heater is by the thermostatic control module controls, can guarantee the reaction temperature equilibrium of reaction cup, simultaneously, the utility model is provided with some hollow outs to the axis connecting hole to the middle part of carriage, satisfy under the requirement of entire reaction dish response intensity in assurance, saved the material of entire reaction dish, make that the cost of reaction tray is controlled preferably, and be provided with 45～90 reaction cup carriages, detect through a serum, can quantitatively detect the concentration of nearly 20 kinds of tumor markerses, detect CV＜10%, and noiseless between the index, and specificity is good.
Description of drawings
Fig. 1 is a reaction tray structural representation described in the utility model.
Fig. 2 is a biochip analysis instrument structural representation described in the utility model.
The specific embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described in further details:
As depicted in figs. 1 and 2, a kind of biochip analysis instrument comprises main control system, the display that is electrically connected with main control system, bearing substrate; Described main control system comprises mainboard and is arranged at the CPU of mainboard, described bearing substrate is provided with seroreaction dish 1, reactant liquor reaction tray 2, luminescent solution reaction tray 3, the first multi freedom degree mechanical hand (R1 mechanical arm) 51, the second multi freedom degree mechanical hand (R2 mechanical arm) 52 and ccd image harvester and darkroom 4, seroreaction dish 1, reactant liquor reaction tray 2, luminescent solution reaction tray 3, R1 mechanical arm 51, R2 mechanical arm 52 and ccd image harvester and darkroom 4 (described ccd image harvester and darkroom 4 settings are interior with darkroom and CCD guiding mechanism 17) are electrically connected with CPU by control module separately.Simultaneously, present embodiment also comprises the chip automatic flushing device 171 that is arranged between seroreaction dish 1 and the reactant liquor reaction tray 2, and the chip automatic flushing device II72 between reactant liquor reaction tray 2 and the luminescent solution reaction tray; Be used for reagent automatic filling device and reaction cup automatic flushing device that reactant liquor reaction unit and luminescent solution reaction unit add reagent, described chip automatic flushing device, reagent automatic filling device and automatic flushing device are electrically connected with CPU by control module.
The control module of seroreaction dish 1, reactant liquor reaction tray 2, luminescent solution reaction tray 3 is motion control card I, the control module of R1 mechanical arm and R2 mechanical arm is motion control card II, the control module of described chip automatic flushing device, reagent automatic filling device and automatic flushing device is a data collecting card, and described image processing apparatus control module is an image pick-up card; Also comprise the temperature control modules that is used to control reacting liquid temperature, described temperature control modules is electrically connected with CPU.
The seroreaction dish 1 and the reactant liquor reaction tray 2 of this enforcement all can adopt reaction tray described in the utility model, comprise the axis connecting hole, some circular housings 10 that are used to support reaction cup, described axis connecting hole and carriage are formed in one, described reaction tray carriage 10 detachably is fastenedly connected by screw thread with axis, and be connected to drive motors 11 by axis, drive the rotation of reaction tray carriage by drive motors.Carriage is provided with 45～90 reaction cup fixed bits 15, evenly equidistantly distributes, and present embodiment is preferably 60.Also comprise chip position detecting sensor 16, sensor probe is arranged at reaction cup fixed bit middle part or approaches bottom position, and this chip position detecting sensor is fixed under the heating ring 13.Also comprise the reaction cup heating ring 13 and the bracket plate 14 that are used for reaction cup heating, described reaction cup heating ring 13 is arranged at a little less than the reaction cup middle part with upper/lower positions, and described bracket plate 14 detachably is fastenedly connected with carriage.Also comprise harmonic wave speed reducing machine assembly 12, be arranged at drive motors 11 and add between the hot charging ring 13, be electrically connected,, be used to control the rotary speed of reaction bracket by the control module control of reaction instrument with drive motors.Carriage and/or reaction cup are provided with mechanical counnter attack plug device.The axis connecting hole is following concave curved surface to the middle part of carriage.Reaction tray and axis detachably are fastenedly connected the position and are provided with some hollow outs to the middle part at carriage position.The luminescent solution reaction tray is provided with 10 carriages, placing response cup on the carriage, the biochip of slide matrix is placed in the reaction cup, various reactant liquors and luminescent solution are filled in the reaction cup automatically, antigen-antibody reaction is finished at chip surface, two manipulators are finished the relevant position of chip between each reaction tray and luminosity scale and are cleaned, dry up and handle, produce chemiluminescence reaction, utilize charge-coupled device (CCD) camera measured light intensity, and use imaging technique to measure the output quantity of biochip surface response location light.Biochip mobile picks and places between each working region, the reaction cup waste treatment finishes automatically by R1 mechanical arm and R2 mechanical arm.
Seroreaction dish, reactant liquor reaction tray and luminescent solution reaction tray are mainly used in the carrying reaction cup, and in the correct time reaction cup are moved to chip and put into, the reactant liquor filling, and the reaction cup cleaning waits each operating position, and reaction cup is carried out corresponding operation.At the reaction cup put area heating ring is arranged, reaction cup is heated.Reaction tray directly is connected on the load-bearing mainboard of frame, is in the environment of a relative closure, makes temperature relatively stable, and the temperature that guarantees all course of reaction chips and reactant liquor is all in the temperature province of regulation.The calibration action of reaction tray realizes by the high speed reducing ratio harmonic speed reducer of AC servo machinery driving high accuracy, to guarantee the accuracy of reaction cup position.
R1 mechanical arm and R2 mechanical arm are used for the transmission of chip and abandoning of discarded object, and chip in the clamping of chip cleaning process and clean, and R1 mechanical arm, R2 mechanical arm structure type are the two degrees of freedom manipulator.The R1 mechanical arm directly is connected on the load-bearing mainboard of frame, between seroreaction dish and reactant liquor reaction tray; The R1 mechanical arm directly is connected on the load-bearing mainboard of frame, and main joint is a rotating shaft between luminescent solution reaction tray and reactant liquor reaction tray, is lifting shaft from the joint, and two joints all adopt the mode of synchronous band of stepper motor driving and synchronizing wheel to realize predetermined action.The end of mechanical arm is a pneumatic jaw, is used to realize gripping and unclasps two actions.
The chip cleaning device cleans the residual reactants matter on the biochip, in order to avoid influence the reaction effect of next step course of reaction after being used for the course of reaction end.The chip cleaning device directly is connected on the load-bearing mainboard of frame, is positioned near seroreaction dish and the luminescent solution reaction tray.Every group of cleaning needle has the two rows needle tubing, the filling of cleaning agent by diaphragm pump under arrange needle tubing and go out, compressed air is blown to the biochip after cleaning by last row's needle tubing, to remove the cleaning fluid of chip surface.
Reactant liquor filling unit and luminescent solution filling unit are used for the accurate filling of reactant liquor and luminescent solution.Reactant liquor filling unit and luminescent solution filling unit directly are connected on the load-bearing mainboard of frame and are positioned near seroreaction dish or the luminescent solution reaction tray, and its structure type is the single-degree-of-freedom manipulator, and drive unit is a cylinder; Cell end is equipped with a filling syringe needle, and the filling syringe needle stretches in the reaction cup during filling, by the micro-precision filling of a delicate metering diaphragm pump realization response reagent, adding amount required precision ± 5%.
The course of reaction that is mainly used in the reaction cup cleaning device is cleaned the residual reactants matter in the reaction cup, in order to avoid influence the reaction effect of course of reaction next time after finishing.The reaction cup cleaning unit directly is connected on the load-bearing mainboard of frame, is positioned near reactant liquor reaction tray and the luminescent solution reaction tray, and its structure type is the single-degree-of-freedom manipulator, and drive unit is a cylinder; Cell end is equipped with one and cleans syringe needle, and the filling syringe needle stretches in the reaction cup during filling, and each cleans on the syringe needle two needle tubings, and a needle tubing is used for the filling of cleaning agent, is realized the micro-precision filling of cleaning agent by a delicate metering diaphragm pump; Another is used for the absorption of waste liquid, produces vacuum by a diaphragm pump in pipeline, realizes drawing the waste liquid function.
Present embodiment also is provided with operating desk, reaction cup abandoned stope and chip abandoned stope, and reaction cup abandoned stope and chip abandoned stope are used for course of reaction and finish the collection of back to disposable discarded object.Reaction cup abandoned stope and chip abandoned stope comprise the pipeline that is fixed on the load-bearing mainboard and are positioned over the waste material box of welding stand inside, mechanical arm on the reaction tray gripping behind the disposable discarded object, move to the abandoned stope top, unclamp jaw, disposable discarded object is fallen in the waste material box by pipeline.
Because reactant liquor and luminescent solution need be preserved under 4-8 ℃ of condition,, present embodiment operating desk specially is provided with small refrigerator 8, is used to preserve reactant liquor and luminescent solution.Simultaneously, can reach ± 0.5 ℃ temperature control precision by the intelligent temperature control module.All courses of reaction all require to carry out temperature control precision under 30-37 ℃ of condition: ± 0.5 ℃.In the luminescent solution course of reaction, carry out twice and take continuously, the time for exposure is respectively 10s, 30s.By the fluorescence on the biochip being taken the area of the light-emitting zone of analysis of fluorescence and brightness.
Present embodiment is divided into seroreaction dish, reactant liquor reaction tray and luminescent solution reaction tray, seroreaction dish and reactant liquor reaction tray are respectively equipped with 60 carriages, the luminescent solution reaction tray is provided with 10 carriages, carriage and reaction cup be provided with mechanical counnter attack plug device can effectively prevent the misplug of reaction cup and biochip towards inconsistent.The axis connecting hole is following concave curved surface to the middle part of carriage, placing response cup on the carriage, the biochip of slide matrix is placed in the reaction cup, various reactant liquors and luminescent solution are filled in the reaction cup automatically, antigen-antibody reaction is finished at chip surface, two manipulators are finished the relevant position of chip between each reaction tray and luminosity scale and are cleaned, dry and handle, produce chemiluminescence reaction, utilize charge-coupled device (CCD) camera measured light intensity, and use imaging technique to measure the output quantity of biochip surface response location light.
Above content be in conjunction with concrete preferred implementation to further describing that the utility model is done, can not assert that concrete enforcement of the present utility model is confined to these explanations.For the utility model person of an ordinary skill in the technical field, under the prerequisite that does not break away from the utility model design, can also make some simple deduction or replace, all should be considered as belonging to the protection domain of utility model.
Claims (10)
1. reaction tray is characterized in that: comprise the axis connecting hole, and some carriages that are used to support reaction cup, described axis connecting hole and carriage are formed in one, and described reaction tray and axis detachably are fastenedly connected, and are connected to drive motors by axis.
2. reaction tray as claimed in claim 1, it is characterized in that: also comprise the reaction cup heater and the bracket plate that are used for the reaction cup heating, described reaction cup heater is arranged at a little less than reaction cup middle part with upper/lower positions, and described bracket plate and carriage detachably are fastenedly connected.
3. reaction tray as claimed in claim 2 is characterized in that: also comprise deceleration device, described deceleration device is arranged between drive motors and the heater, and this deceleration device is electrically connected with drive motors.
4. reaction tray as claimed in claim 3 is characterized in that: described deceleration device is the harmonic wave speed reducing machine assembly.
5. as each described reaction tray of claim 1～4, it is characterized in that: described carriage is provided with 45～90 reaction cup fixed bits, evenly equidistantly distributes.
6. reaction tray as claimed in claim 5, it is characterized in that: also comprise the chip position detecting sensor, described sensor probe is arranged at reaction cup fixed bit middle part or approaches bottom position, and this chip position detecting sensor is fixed under the heater.
7. reaction tray as claimed in claim 6 is characterized in that: described carriage and/or reaction cup are provided with mechanical counnter attack plug device.
8. reaction tray as claimed in claim 7 is characterized in that: described axis connecting hole is following concave curved surface to the middle part of carriage.
9. reaction tray as claimed in claim 8 is characterized in that: described reaction tray and axis detachably are fastenedly connected by screw thread.
10. reaction tray as claimed in claim 9 is characterized in that: described reaction tray is for circular.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202059305U CN201524578U (en) | 2009-10-19 | 2009-10-19 | Reaction tray |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202059305U CN201524578U (en) | 2009-10-19 | 2009-10-19 | Reaction tray |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201524578U true CN201524578U (en) | 2010-07-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009202059305U Expired - Lifetime CN201524578U (en) | 2009-10-19 | 2009-10-19 | Reaction tray |
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| Country | Link |
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| CN (1) | CN201524578U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102636636A (en) * | 2011-02-09 | 2012-08-15 | 三星电子株式会社 | Disk type microfluidic device |
| CN104428064A (en) * | 2012-07-09 | 2015-03-18 | 拜奥卡蒂斯股份有限公司 | Heater for a disposable diagnosis cartridge |
| CN108637567A (en) * | 2018-05-29 | 2018-10-12 | 苏州博宇科技有限公司 | A kind of anti-mounted welding fixture of electrically conductive weldment |
| CN108704681A (en) * | 2018-05-25 | 2018-10-26 | 辽宁工业大学 | A kind of instant-plugging micro-fluidic chip securing device |
| CN114113048A (en) * | 2021-11-23 | 2022-03-01 | 南京晶捷生物科技有限公司 | Optical detection equipment |
-
2009
- 2009-10-19 CN CN2009202059305U patent/CN201524578U/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102636636A (en) * | 2011-02-09 | 2012-08-15 | 三星电子株式会社 | Disk type microfluidic device |
| CN104428064A (en) * | 2012-07-09 | 2015-03-18 | 拜奥卡蒂斯股份有限公司 | Heater for a disposable diagnosis cartridge |
| CN104428064B (en) * | 2012-07-09 | 2017-03-22 | 拜奥卡蒂斯股份有限公司 | Heater for a disposable diagnosis cartridge |
| CN108704681A (en) * | 2018-05-25 | 2018-10-26 | 辽宁工业大学 | A kind of instant-plugging micro-fluidic chip securing device |
| CN108637567A (en) * | 2018-05-29 | 2018-10-12 | 苏州博宇科技有限公司 | A kind of anti-mounted welding fixture of electrically conductive weldment |
| CN114113048A (en) * | 2021-11-23 | 2022-03-01 | 南京晶捷生物科技有限公司 | Optical detection equipment |
| CN114113048B (en) * | 2021-11-23 | 2023-10-27 | 南京晶捷生物科技有限公司 | Optical detection equipment |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20100714 |