CN108593939B - Full-automatic protein chip and application thereof - Google Patents
Full-automatic protein chip and application thereof Download PDFInfo
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- CN108593939B CN108593939B CN201810791629.0A CN201810791629A CN108593939B CN 108593939 B CN108593939 B CN 108593939B CN 201810791629 A CN201810791629 A CN 201810791629A CN 108593939 B CN108593939 B CN 108593939B
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 119
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 119
- 238000003466 welding Methods 0.000 claims abstract description 40
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- 238000000034 method Methods 0.000 claims description 17
- 238000001514 detection method Methods 0.000 claims description 16
- 239000000427 antigen Substances 0.000 claims description 13
- 102000036639 antigens Human genes 0.000 claims description 13
- 108091007433 antigens Proteins 0.000 claims description 13
- 230000009870 specific binding Effects 0.000 claims description 13
- 230000004927 fusion Effects 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 10
- 238000009739 binding Methods 0.000 claims description 6
- 239000003085 diluting agent Substances 0.000 claims description 5
- 229920001169 thermoplastic Polymers 0.000 claims description 4
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- 201000010099 disease Diseases 0.000 abstract description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 11
- 238000003745 diagnosis Methods 0.000 abstract description 6
- 230000007547 defect Effects 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 13
- 239000000523 sample Substances 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
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- 238000003759 clinical diagnosis Methods 0.000 description 2
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 238000003317 immunochromatography Methods 0.000 description 2
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- 208000035473 Communicable disease Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
Abstract
The application belongs to the technical field of disease diagnosis, and particularly relates to a full-automatic protein chip and application thereof. The application provides a full-automatic protein chip, which comprises: a tooth-shaped upper cover, a tooth-shaped lower cover, a fuse wire, a welding groove, a front and back marking port and a reaction film; the bottoms of the tooth-shaped upper cover and the tooth-shaped lower cover are provided with gaps, and the reaction film is fixed in the gaps at the bottoms of the tooth-shaped upper cover and the tooth-shaped lower cover; the tooth-shaped lower cover is provided with a welding groove, and the tooth-shaped upper cover is provided with a fuse wire, so that the fuse wire is contacted with the welding groove for welding when dissolved; the top ends of the tooth-shaped upper cover and the tooth-shaped lower cover are respectively provided with a front and back marking opening, and when the tooth-shaped upper cover and the tooth-shaped lower cover are welded, the front and back marking openings of the tooth-shaped upper cover and the tooth-shaped lower cover are mutually aligned; wherein, the fuse wire is of a convex structure. The application solves the technical defect that the current protein chip cannot be used for a full-automatic machine.
Description
Technical Field
The application belongs to the technical field of disease diagnosis, and particularly relates to a full-automatic protein chip and application thereof.
Background
Many malignant diseases such as tumors, malignant infectious diseases and the like have greatly increased mortality rate if not diagnosed early, and the consequences are not considered. Existing disease diagnosis methods include chemical diagnosis methods and physical diagnosis methods. Because physical methods cannot diagnose early diseases and require a large amount of instruments and equipment and a certain requirement for environment and high cost, the multi-purpose chemical method detects the content change of the disease-related protein or small molecular substance in the patient to diagnose the diseases.
However, the current chemical methods for detecting diseases, such as ELISA, radioisotope labeling, gold labeling, fluorescence, chemiluminescence, time-resolved fluorescence, and the like, are mostly single-index detection, have low confidence level, and have limitations, such as complex labeling steps, expensive instruments and reagents, complicated operation, low sensitivity, and easy pollution. Because the protein chip technology can make up the limitation of the chemical detection method, the protein chip technology is an emerging technology of multi-index parallel detection developed in recent years, has the characteristics of high flux and high information content, and has wide application prospect in the fields of scientific research and clinical diagnosis. However, due to technical and cost factors, practical application is limited, and the method has not been popularized in the clinical diagnosis field. At present, a low-density protein chip which has low cost, no complex and expensive equipment, sensitivity and accuracy, can rapidly perform multiplex detection once, is simple and convenient to operate and is suitable for rapid point-of-care detection (POCT) is an important research and development direction in the field of protein chips.
For the last 20 years, the development and production of immunodetection reagents for disease monitoring and diagnosis have been widely applied to rapid point of care (POCT) as represented by lateral flow immunochromatography, diafiltration, and spot methods, and rapid detection field labeling techniques have been diversified with the development of enzyme development, colloidal gold, colloidal selenium, fluorescent microspheres, chemiluminescence, quantum dots, and other more sensitive labeling techniques; due to the mature application of these techniques and their advantages and practicality in rapid detection, they are also applied to the research and development of protein chips.
At present, the type is based on immunochromatography, percolation and spot method technologies, protein chip products with POCT application as targets have greatly progressed in technology and application, but traditional glass slides, silicon wafers and the like are used as protein chips of chip substrate types, the operation is complex, and a reaction film on the protein chips is simply fixed on the protein chips, so that the reaction film can only be directly arranged on the chip substrate to react with a sample, and the application of the protein chips in full automation is greatly realized; moreover, the structure and design of the protein chip have considerable limitations, and the protein chip is difficult to grasp and transfer by a fully-automatic machine, so that a plurality of protein chips cannot be used in the fully-automatic machine currently.
Therefore, in summary, developing a protein chip that can be used in a fully automated machine and has low cost is a technical problem to be solved by those skilled in the art.
Content of the application
In view of the above, the application provides a fully automatic protein chip and application thereof, which can effectively solve the technical defect that the current protein chip cannot be used for a fully automatic machine.
The application provides a full-automatic protein chip, which comprises: a tooth-shaped upper cover, a tooth-shaped lower cover, a fuse wire, a welding groove, a front and back identification notch and a reaction film;
the bottoms of the tooth-shaped upper cover and the tooth-shaped lower cover are provided with gaps, and the reaction film is fixed in the gaps of the bottoms of the tooth-shaped upper cover and the tooth-shaped lower cover;
the edge of the notch of the tooth-shaped lower cover is provided with a welding groove, the edge of the notch of the tooth-shaped upper cover is provided with a fuse wire, and when the fuse wire is dissolved, the fuse wire is welded with the welding groove, so that the reaction film is fixed between the tooth-shaped upper cover and the notch of the tooth-shaped lower cover;
the top ends of the tooth-shaped upper cover and the tooth-shaped lower cover are respectively provided with a front and back identification notch, when the tooth-shaped upper cover and the tooth-shaped lower cover are welded, the front and back identification notches of the tooth-shaped upper cover and the tooth-shaped lower cover are mutually aligned, and the front and back identification notches are used for distinguishing the front and back of the reaction film;
wherein, the fuse wire is of a convex structure.
Further, the protruding structure is a triangular protruding structure.
Wherein the tooth-shaped upper cover, the fuse wire and the front and back surface identification notches of the tooth-shaped upper cover are integrally formed; the tooth-shaped lower cover, the welding groove and the front and back identification notches of the tooth-shaped lower cover are integrally formed.
Preferably, the reaction membrane is immobilized with at least 1 antibody or antigen.
Preferably, the dental upper cover and the dental lower cover are welded according to an ultrasonic welding technique.
Preferably, the full-automatic protein chip further comprises a limiting hole and a limiting protrusion;
the tooth-shaped lower cover is provided with the limiting protrusions, and the tooth-shaped upper cover is provided with the limiting holes, so that the limiting protrusions of the tooth-shaped lower cover are arranged in the limiting holes of the tooth-shaped upper cover to limit.
Wherein the tooth-shaped lower cover and the limit protrusion are integrally formed.
Preferably, the tooth-shaped upper cover, the tooth-shaped lower cover, the fuse wire and the welding groove are all made of thermoplastic plastics.
Furthermore, the fully-automatic protein chip provided by the application adopts an ultrasonic welding machine to quickly fuse the tooth-shaped upper cover and the tooth-shaped lower cover together.
Further, the time is set such that the ultrasound is stopped immediately upon contact of the upper chip cavity face with the chip. Vibration and heat are instantaneous high temperatures within the circled area of the fuse. Will not pass onto the chip. What absorbs ultrasonic energy is this fuse, which is timed such that the chip above comes to a stop immediately upon contact with the chip. The heat is the instantaneous high temperature above the fuse.
The application also discloses a full-automatic protein chip analysis system, which comprises: full-automatic protein chip and reaction cup;
the reaction cup comprises a cup body;
the full-automatic protein chip is sleeved in the cup body of the reaction cup.
Preferably, both sides of the tooth-shaped upper cover are also provided with limit edges; limiting edges are further arranged on two sides of the tooth-shaped lower cover, so that the limiting edges of the tooth-shaped upper cover and the limiting edges of the tooth-shaped lower cover are aligned with each other;
the reaction cup further comprises a limiting groove, wherein the limiting groove is arranged on an opening of the cup body of the reaction cup, so that limiting edges on two sides of the tooth-shaped lower cover and the tooth-shaped upper cover are placed in the limiting groove to limit.
The tooth-shaped upper cover and the tooth-shaped lower cover are integrally formed and manufactured; the limiting groove and the cup body of the reaction cup are integrally formed.
Preferably, the reaction membrane of the fully automatic protein chip is fixedly provided with at least 1 antibody or antigen.
The application of the full-automatic protein chip in antigen-antibody specific binding provided by the application comprises the following steps:
s101: adding a sample to be tested or a sample to be tested and a diluent into the reaction cup;
s102: placing the full-automatic protein chip in a reaction cup of S101 to perform antigen-antibody specific binding;
s103: after the antigen-antibody specific binding reaction is finished, the detection result is read by a detection reader after washing the full-automatic protein chip and incubating the full-automatic protein chip with the enzyme-labeled secondary antibody.
Preferably, the reaction membrane of the fully automatic protein chip is fixedly provided with at least 1 antibody or antigen.
Further, the antibodies or antigens immobilized on the reaction membrane are arranged and immobilized in a matrix.
Further, the antibody or antigen immobilized by the reaction membrane is a qualitatively detected antibody or antigen or/and a quantitatively detected antibody or antigen.
Wherein, when the tooth-shaped upper cover and the tooth-shaped lower cover are welded, the front and back identification notches of the tooth-shaped upper cover and the tooth-shaped lower cover are mutually aligned; the limiting protrusions of the tooth-shaped lower cover are mutually aligned with the limiting holes of the tooth-shaped upper cover, and the limiting protrusions of the tooth-shaped lower cover are arranged in the limiting holes of the tooth-shaped upper cover for limiting; the limit edges on two sides of the tooth-shaped lower cover and the tooth-shaped upper cover are mutually aligned, and the limit edges on two sides of the tooth-shaped lower cover and the tooth-shaped upper cover are placed in the limit grooves for limiting.
The application provides a full-automatic protein chip, which comprises: a tooth-shaped upper cover, a tooth-shaped lower cover, a fuse wire, a welding groove, a front and back identification notch and a reaction film; the bottoms of the tooth-shaped upper cover and the tooth-shaped lower cover are provided with gaps, and the reaction film is fixed in the gaps at the bottoms of the tooth-shaped upper cover and the tooth-shaped lower cover; the edge of the notch of the tooth-shaped lower cover is provided with a welding groove, the edge of the notch of the tooth-shaped upper cover is provided with a fuse wire, and when the fuse wire is dissolved, the fuse wire is welded with the welding groove, so that the reaction film is fixed between the notch of the tooth-shaped upper cover and the notch of the tooth-shaped lower cover. The fuse wire is a convex structure formed on the edge of the notch of the tooth-shaped upper cover, the basic function of the fuse wire is to gather energy (absorb ultrasonic energy) so that the fuse wire can reach the melting temperature as soon as possible, and the high temperature can not be transmitted to the chip, thereby obtaining better welding effect, therefore, the reaction film is firmly fixed in the notches at the bottoms of the tooth-shaped upper cover and the tooth-shaped lower cover, the reaction film of the protein chip can react with a sample in a suspension manner, and the reaction film is not easy to fall off when the protein chip is applied to a full-automatic machine; the front and back sign notch is all equipped with on the top of tooth form upper cover and tooth form lower cover, and when tooth form upper cover and tooth form lower cover butt fusion, the front and back sign notch of tooth form upper cover and tooth form lower cover aligns each other, and front and back sign notch is used for distinguishing the front and back of reaction membrane, can guarantee the accuracy of the data analysis of reaction membrane. The structure of the full-automatic protein chip is tooth-shaped, the reaction film is fixed in a notch at the bottom of the tooth-shaped structure, the top end of the full-automatic protein chip can be grabbed by a full-automatic machine to suspend the reaction film, on one hand, the full-automatic machine can conveniently grab and transfer the protein chip, on the other hand, the reaction film cannot be contacted or destroyed, the protein chip is convenient to grab and simple in structure, the full-automatic protein chip can react with antigen-antibody only by moving the full-automatic protein chip into a sample solution by the full-automatic machine, the operation is simple, and meanwhile, the full-automatic protein chip is simple to prepare and low in cost.
The application of the full-automatic protein chip in antigen-antibody specific binding provided by the application comprises the following steps: the reaction cup comprises a cup body, and the full-automatic protein chip is sleeved in the cup body of the reaction cup. When the system is applied, the method comprises the following steps: s101: adding a sample to be tested or a sample to be tested and a diluent into the reaction cup; s102: placing the full-automatic protein chip in a reaction cup of S101 to perform antigen-antibody specific binding; s103: after the antigen-antibody specific binding reaction is finished, the detection result is read by a detection reader after washing the full-automatic protein chip and incubating the full-automatic protein chip with the enzyme-labeled secondary antibody. Therefore, the full-automatic protein chip is hung and transferred through the top end of the full-automatic machine for grabbing the protein chip, so that the full-automatic protein chip is placed in a reaction cup containing a sample and a reaction cup containing a second antibody marked by enzyme, antigen-antibody specific binding reaction occurs, different diseases are detected simultaneously, the full-automatic protein chip can be used in the full-automatic machine, and the full-automatic protein chip and the reaction cup are simple in structure and low in preparation cost.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below.
FIG. 1 shows a schematic diagram of the full-automatic protein chip provided by the application;
FIG. 2 is a schematic diagram of a fully automatic protein chip after fusion;
FIG. 3 shows a top view of a fully automated protein chip provided by the present application;
FIG. 4 is a schematic diagram showing a fusion process of a fully-automatic protein chip provided by the application;
FIG. 5 shows a perspective view of a fully automatic protein chip provided by the application;
FIG. 6 is a perspective view showing the assembly of a fully automatic protein chip and a reaction cup according to the present application;
FIG. 7 is an assembled front view of a fully automated protein chip and cuvette according to the present application;
FIG. 8 shows a workflow diagram of an application of the fully automated protein chip analysis system of the present application;
FIG. 9 shows a specific workflow diagram of an application of the fully automated protein chip analysis system of the present application;
wherein, 1 is tooth form lower cover, 2 is tooth form upper cover, 3 is spacing hole, 4 is the fuse, 5 is positive and negative sign notch, 6 is breach, 7 is the reaction membrane, 8 is the reaction cup, 9 is the welding recess, 10 is spacing recess, 11 is spacing limit, A is the structure diagram before the full-automatic protein chip welds, B, C, D is full-automatic protein chip welds structure diagram respectively, E is the structure diagram when full-automatic protein chip welds and accomplishes.
Detailed Description
The application provides a full-automatic protein chip and application thereof, which are used for solving the technical defect that the protein chip cannot be used for a full-automatic machine in the prior art.
The following description of the technical solutions in the embodiments of the present application will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
The reagents and materials of the examples of the present application are commercially available.
Wherein, the second antibody marked by enzyme is called enzyme-labeled secondary antibody for short; CCD imaging technology is called CCD imaging for short.
Example 1
Referring to fig. 1, an embodiment 1 of the present application is a fully automatic protein chip, where embodiment 1 includes: a tooth-shaped lower cover 1, a tooth-shaped upper cover 2, a fuse wire 4, a front and back mark notch 5, a reaction film 7 and a welding groove 9; the bottoms of the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1 are provided with notches 6, and the reaction membrane 7 is fixed in the notches 6 at the bottoms of the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1; the edge of the notch 6 of the tooth-shaped lower cover 1 is provided with a welding groove 9, the edge of the notch 6 of the tooth-shaped upper cover 2 is provided with a fuse guide 4, and when the fuse guide 4 is dissolved, the fuse guide is welded with the welding groove 9, so that the reaction film 7 is fixed between the tooth-shaped upper cover 2 and the notch 6 of the tooth-shaped lower cover 1; the tops of the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1 are respectively provided with a front and back identification notch 5, when the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1 are welded, the front and back identification notches 5 of the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1 are mutually aligned, and the front and back identification notches 5 are used for distinguishing the front and back of the reaction membrane 7.
Further, the fuse wire 4 has a convex structure.
In embodiment 1, the edge of the notch of the tooth-shaped lower cover 1 is provided with a welding groove 9, the edge of the notch of the tooth-shaped upper cover 2 is provided with a fuse 4, when the fuse 4 is dissolved, the fuse 4 is welded with the welding groove 9, so that the reaction film 7 is fixed between the tooth-shaped upper cover 2 and the notch of the tooth-shaped lower cover 1, the fuse 4 is of a convex structure, when dissolved, the convex fuse 4 can gather energy, the instant high temperature is reached, and the instant high temperature is not transferred to the reaction film 7, the instant high temperature fuse 4 is welded with the welding groove 9, and the reaction film 7 is firmly fixed between the tooth-shaped upper cover 2 and the notch 6 of the tooth-shaped lower cover 1. Therefore, the top end of the full-automatic protein chip can be hung by a full-automatic machine to react with different reaction liquids, so that the full-automatic application of the protein chip is realized. In addition, the front and back identification notch is used for distinguishing the front and back of the reaction membrane, so that the accuracy of data analysis of the reaction membrane can be ensured.
Example 2
Referring to fig. 1 to 7, embodiment 1 of the present application is a fully automatic protein chip, and embodiment 2 includes: the device comprises a tooth-shaped lower cover 1, a tooth-shaped upper cover 2, a fuse guide 4, a front and back mark notch 5, a limiting hole 3, a reaction film 7 and a welding groove 9; the bottoms of the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1 are provided with notches 6, and the reaction membrane 7 is fixed in the notches 6 at the bottoms of the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1; the edge of the notch 6 of the tooth-shaped lower cover 1 is provided with a welding groove 9, the edge of the notch 6 of the tooth-shaped upper cover 2 is provided with a fuse guide 4, and when the fuse guide 4 is dissolved, the fuse guide is welded with the welding groove 9, so that the reaction film 7 is fixed between the tooth-shaped upper cover 2 and the notch of the tooth-shaped lower cover 1; the top ends of the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1 are respectively provided with a front and back identification notch 5, when the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1 are welded, the front and back identification notches 5 of the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1 are mutually aligned, and the front and back identification notches 5 are used for distinguishing the front and back of the reaction film 7; the tooth-shaped lower cover is provided with a limiting protrusion, and the tooth-shaped upper cover is provided with a limiting hole 3, so that the limiting protrusion of the tooth-shaped lower cover is arranged in the limiting hole 3 of the tooth-shaped upper cover for limiting.
Further, the fuse wire 4 has a triangular protruding structure.
The triangular protruding structure is used for gathering energy, so that the energy can reach the melting temperature as soon as possible, and a better welding effect is obtained.
Further, the reaction membrane is immobilized with at least 1 antibody or antigen.
Further, when the dental upper cover and the dental lower cover are welded according to the ultrasonic welding technique.
Further, the tooth-shaped upper cover, the tooth-shaped lower cover, the fuse wire and the welding groove are all made of thermoplastic plastics.
Example 2 the welding was performed using an ultrasonic welding technique, the thermoplastic fuse 4 absorbed ultrasonic energy, and the ultrasonic welding time was set such that when the fuse 4 and the welding groove 9 were in contact with each other, the ultrasonic welding was stopped immediately, so that the fuse 4 reached an instantaneous high temperature and contacted the welding groove 9, and the high temperature was prevented from being transferred to the reaction film.
As can be seen from the schematic diagram of the fully automatic protein chip after fusion in fig. 2 and the top view of the fully automatic protein chip in fig. 3, a fusion groove 9 is provided at the edge of the notch of the dental lower cover 1, a fuse wire 4 is provided at the edge of the notch of the dental upper cover 2, and when the fuse wire 4 is dissolved, the fusion groove 9 is fused to fix the reaction film 7 between the notch of the dental upper cover 2 and the notch of the dental lower cover 1, the fusion process occurs at the edges of the notch of the dental upper cover 2 and the dental lower cover 1, and the fused high temperature is not transmitted to the chip; the detailed process of fusion is shown in fig. 4, fig. 4 is a schematic diagram of the fusion process of the fully automatic protein chip, a is a structure diagram before fusion of the fully automatic protein chip, B, C, D is a structure diagram between fusion of the fully automatic protein chip, E is a structure diagram when fusion of the fully automatic protein chip is completed, and it is known that the dental upper cover 2 and the dental lower cover 1 are tightly fixed together after fusion, and the reaction membrane 7 is firmly clamped.
Example 3
Referring to fig. 1 to 7, embodiment 1 of the present application is a fully automatic protein chip analysis system, and embodiment 3 includes: the device comprises a tooth-shaped lower cover 1, a tooth-shaped upper cover 2, a fuse wire 4, a front and back surface mark notch 5, a limiting hole 3, a reaction cup 8, a limiting edge 11, a limiting groove 10, a reaction film 7 and a welding groove 9; the bottoms of the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1 are provided with notches 6, and the reaction membrane 7 is fixed in the notches 6 at the bottoms of the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1; the edge of the notch 6 of the tooth-shaped lower cover 1 is provided with a welding groove 9, the edge of the notch 6 of the tooth-shaped upper cover 2 is provided with a fuse guide 4, and when the fuse guide 4 is dissolved, the fuse guide is welded with the welding groove 9, so that the reaction film 7 is fixed between the tooth-shaped upper cover 2 and the notch of the tooth-shaped lower cover 1; the top ends of the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1 are respectively provided with a front and back identification notch 5, when the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1 are welded, the front and back identification notches 5 of the tooth-shaped upper cover 2 and the tooth-shaped lower cover 1 are mutually aligned, and the front and back identification notches 5 are used for distinguishing the front and back of the reaction film 7; the tooth-shaped lower cover is provided with a limiting protrusion, and the tooth-shaped upper cover is provided with a limiting hole 3, so that the limiting protrusion of the tooth-shaped lower cover is arranged in the limiting hole 3 of the tooth-shaped upper cover for limiting; the reaction cup 8 comprises a cup body, and a full-automatic protein chip is sleeved in the cup body of the reaction cup 8; the limiting edges 11 are arranged on two sides of the tooth-shaped upper cover and the tooth-shaped lower cover, so that the limiting edges 11 of the tooth-shaped upper cover and the limiting edges 11 of the tooth-shaped lower cover are mutually aligned; the limiting groove 10 is arranged on the opening of the cup body, so that the limiting edges 11 on two sides of the tooth-shaped upper cover and the tooth-shaped lower cover are placed in the limiting groove 10 for limiting.
At least 1 antibody or antigen is fixedly arranged on the reaction membrane of the full-automatic protein chip.
Different solutions can be added into the reaction cup 8 of embodiment 3, and the fully automatic protein chip can be suspended and transferred into the reaction cup 8 by a fully automatic machine, so that the fully automatic protein chip reacts with the different solutions, and the limit edges 11 on both sides of the tooth-shaped upper cover and the tooth-shaped lower cover are placed on the limit grooves 10 of the opening of the cup body, so that the reaction film can better contact with the solution in the reaction cup 8 to react.
Example 4
Referring to fig. 8, fig. 8 shows an application of the fully automatic protein chip analysis system of the present application, and embodiment 4 includes the following steps:
s101: adding a sample to be tested or a sample to be tested and a diluent into the reaction cup;
s102: placing the full-automatic protein chip in a reaction cup of S101 to perform antigen-antibody specific binding;
s103: after the antigen-antibody specific binding reaction is finished, the detection result is read by a detection reader after washing the full-automatic protein chip and incubating the full-automatic protein chip with the enzyme-labeled secondary antibody.
The application of the full-automatic protein chip analysis system of example 4, the full-automatic protein chip is suspended and transferred through the top end of the full-automatic machine, which grabs the protein chip, and the chip is placed in a reaction cup containing a sample and a reaction cup containing a second antibody marked by enzyme, so that antigen-antibody specific binding reaction occurs, and thus quantitative or/and qualitative detection is performed on different diseases at the same time, and the full-automatic protein chip can be used in the full-automatic machine.
Example 5
Referring to fig. 9, the present application provides an application embodiment of a fully automatic protein chip, and embodiment 5 is a specific application of the fully automatic protein chip, specifically comprising the following steps:
s1: preparing a reaction cup;
s2: adding a sample to be tested (clinical blood or urine) and a diluent (PBS) into the reaction cup of the S1;
s3: placing a full-automatic protein chip (provided with a detected target antibody) in a reaction cup of S2;
s4: placing the fully-automatic protein chip of S3 and a reaction cup at 37 ℃ for incubation;
s5: the full-automatic protein chip of the S4 is grabbed, suspended and transferred to a washing liquid for washing;
s6: washing the full-automatic protein chip of S5 for 2min;
s7: adding enzyme-labeled secondary antibodies (secondary antibodies specifically combined with the target antibodies) into the new reaction cup;
s8: placing the fully-automatic protein chip washed by the step S6 into a new reaction cup of the step S7;
s9: placing the fully-automatic protein chip of S8 and a new reaction cup at 37 ℃ for incubation;
s10: the full-automatic protein chip of the S9 is grabbed, suspended and transferred to a washing liquid for washing;
s11: washing the fully-automatic protein chip of the S10 for 2min;
s12: placing the fully-automatic protein chip of S11 in a luminescent solution (fluorescence or chemiluminescence solution) for incubation;
s13: draining the moisture of the full-automatic protein chip of the S12;
s14: placing the full-automatic protein chip of the S12 in a CCD imager for photographing and developing;
s15: and (3) carrying out data analysis on the CCD imaging result of the S14 and outputting the result.
Furthermore, the reaction membrane of the full-automatic protein chip is fixedly provided with at least 1 antibody or antigen.
Wherein, the antibodies or antigens of the reaction membrane of the full-automatic protein chip are arranged in a matrix.
The foregoing is merely a preferred embodiment of the present application and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present application, which are intended to be comprehended within the scope of the present application.
Claims (8)
1. A fully automated protein chip comprising: a tooth-shaped upper cover, a tooth-shaped lower cover, a fuse wire, a welding groove, a front and back identification notch and a reaction film;
the bottoms of the tooth-shaped upper cover and the tooth-shaped lower cover are provided with gaps, and the reaction film is fixed in the gaps of the bottoms of the tooth-shaped upper cover and the tooth-shaped lower cover;
the edge of the notch of the tooth-shaped lower cover is provided with a welding groove, the edge of the notch of the tooth-shaped upper cover is provided with a fuse wire, and when the fuse wire is dissolved, the fuse wire is welded with the welding groove, so that the reaction film is fixed between the tooth-shaped upper cover and the notch of the tooth-shaped lower cover;
the top ends of the tooth-shaped upper cover and the tooth-shaped lower cover are respectively provided with a front and back identification notch, when the tooth-shaped upper cover and the tooth-shaped lower cover are welded, the front and back identification notches of the tooth-shaped upper cover and the tooth-shaped lower cover are mutually aligned, and the front and back identification notches are used for distinguishing the front and back of the reaction membrane;
wherein the fuse wire is of a convex structure;
the reaction membrane is fixedly provided with at least 1 antibody or antigen.
2. The fully automatic protein chip of claim 1, wherein the dental upper cover and the dental lower cover are fused according to an ultrasonic fusion technique.
3. The fully automatic protein chip according to any one of claims 1 to 2, further comprising a limiting aperture and a limiting protrusion;
the tooth-shaped lower cover is provided with the limiting protrusions, and the tooth-shaped upper cover is provided with the limiting holes, so that the limiting protrusions of the tooth-shaped lower cover are arranged in the limiting holes of the tooth-shaped upper cover to limit.
4. The fully automatic protein chip according to any one of claims 1 to 2, wherein the dental upper cover, the dental lower cover, the fuse and the fusion groove are all made of thermoplastic.
5. The fully automated protein chip of claim 1, further comprising: a reaction cup;
the reaction cup comprises a cup body;
the full-automatic protein chip is sleeved in the cup body of the reaction cup.
6. The fully automatic protein chip according to claim 5, wherein both sides of the tooth-shaped upper cover are further provided with limit edges; limiting edges are further arranged on two sides of the tooth-shaped lower cover, so that the limiting edges of the tooth-shaped upper cover and the limiting edges of the tooth-shaped lower cover are aligned with each other;
the reaction cup further comprises a limiting groove, wherein the limiting groove is arranged on an opening of the cup body of the reaction cup, so that limiting edges on two sides of the tooth-shaped lower cover and the tooth-shaped upper cover are placed in the limiting groove to limit.
7. Use of a fully automated protein chip according to any one of claims 5 to 6 for antigen-antibody specific binding, comprising the steps of:
s101: adding a sample to be tested or a sample to be tested and a diluent into the reaction cup;
s102: placing the full-automatic protein chip in a reaction cup of S101 to perform antigen-antibody specific binding;
s103: after the antigen-antibody specific binding reaction is finished, the detection result is read by a detection reader after washing the full-automatic protein chip and incubating the full-automatic protein chip with the enzyme-labeled secondary antibody.
8. The use according to claim 7, wherein the reaction membrane of the fully automatic protein chip is immobilized with at least 1 antibody or antigen.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810791629.0A CN108593939B (en) | 2018-07-18 | 2018-07-18 | Full-automatic protein chip and application thereof |
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|---|---|---|---|
| CN201810791629.0A CN108593939B (en) | 2018-07-18 | 2018-07-18 | Full-automatic protein chip and application thereof |
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| CN108593939B true CN108593939B (en) | 2023-11-14 |
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| CN108593939A (en) | 2018-09-28 |
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