CN202794190U - Chip room of protein chip - Google Patents
Chip room of protein chip Download PDFInfo
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- CN202794190U CN202794190U CN 201220485075 CN201220485075U CN202794190U CN 202794190 U CN202794190 U CN 202794190U CN 201220485075 CN201220485075 CN 201220485075 CN 201220485075 U CN201220485075 U CN 201220485075U CN 202794190 U CN202794190 U CN 202794190U
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- absorbent material
- chip
- box body
- water absorbing
- absorbing material
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Abstract
The utility model relates to a chip room of a protein chip. A test is finished in short time by the method of natural percolation in a percolated protein chip, reaction process is uncontrollable, reaction efficiency is low and repeatability of a testing result is poor. The chip room of the protein chip comprises a box body and an upper box cover. A water absorbing material groove frame is arranged in the box body. The water absorbing material groove frame is provided with a water absorbing material groove. The position of the water absorbing material groove corresponds to a reaction window of the upper box cover. Edges of four corners of the water absorbing material groove frame are provided with slope protrusions. A U-shaped tabulate pushing type separating slide plate is arranged above the water absorbing material groove frame. The starting end in the pushing direction is provided with a groove port which is used for clamping the slope protrusions. A chip and water absorbing materials which are in the lower layer are separated by the pushing type separating device arranged in the chip room. Reaction time of an immobilized antigen (antibody) and a tested object can be adjusted and optimized according to experimental requirements when a sample is tested, wherein the immobilized antigen (antibody) and the tested object are reacted in the water absorbing material groove. Reaction efficiency is improved, and repeatability and sensitivity of the testing result are increased.
Description
Technical field
The utility model belongs to biomedical detection technique field, is specifically related to a kind of protein chip core chamber.
Background technology
The biochip technology concept originates from the later stage eighties 20th century, is one of item new technology of recent two decades life science field hot topic, and biochip mainly is divided into genetic chip and albumen two large classes.Protein chip is that albumen or the peptide molecule that will have in a large number differential responses originality are fixed in certain carrier surface formation microarray in an orderly manner by the pattern of rows and columns that tightly designs in advance.Wherein, the percolation protein chip is one of protein chip that is applied to the earliest clinical in vitro diagnosis in vitro, it is a kind of tachysynthesis analytical technology take collaurum or peroxidase (HRP) as high flux, microminiaturization and the robotization of trace labelling thing that grows up on the basis of using for reference DNA chip technology and dot immunogold filtration assay (dot immunogold filtration assay, DIGFA) technology.Its reaction principle is similar to enzyme-linked immunosorbent assay (ELISA), at first specific protein/polypeptide class antigen, antibody or other capture molecules mode with dot matrix is fixed on the film matrix such as nitrocellulose membrane (NC) in an orderly manner, and film chip and water-absorbing material order is stacked in the specific device (core chamber)---be assembled into the percolation protein chip.First test sample is dripped on chip during detection, because chip matrix directly contacts with the indoor absorbent material of core, water absorptivity by absorbent material, sample liquid sees through chip naturally in the diafiltration mode, add the cleansing solution washing and remove not bond, add subsequently the immune colloid gold colour developing, upper machine is read testing result with scanner.Take HRP as label, behind the washing chip, add testing result behind the insoluble chromogenic reagent.
Different according to the kind of immobilization antigen, antibody or other capture molecules and reactive mode, the percolation protein chip can be divided into the reaction patterns such as indirect method, double antibody (former) sandwich method, prize law.
Because that the percolation protein chip has in testing process is easy and simple to handle, quick, sensitive, volume is little and the advantage such as cheap.Therefore, be subject to particularly small towns, community sanitary institute and the epidemic prevention user's of inspection body of basic unit favor of users.
Immune association reaction on the percolation protein chip between immobilised antigen, antibody or other capture molecules and the detected material is that fluid sample is finished through in the short period of film chip in the mode of natural filtration, thereby has the following disadvantages: (1) reaction conditions and course of reaction are uncontrollable; (2) reaction efficiency reduces that (protein chip matrix directly contacts with following absorbent material, the liquid rate of percolation is very fast, cause between the immobilization antigen/antibody or the detected material in other capture molecules and the testing sample on the chip, duration of contact is too short between immune complex marker antibody/antigen that capture molecules and detected material form, cause immune association reaction abundant not, detection sensitivity reduces.Have result of study to show: the ELISA experiment is left standstill under the reaction conditions in non-concussion, immune association reaction curve between certain density Ag-Ab reach plateau (be the two absorption with dissociate reach mobile equilibrium) approximately need 30～40min, we find in early-stage Study, because the concentration lower (being generally 10ng) of immobilised antigen, antibody on the chip, therefore Ag-Ab immunity association reaction time of reaching mobile equilibrium shortens greatly, is approximately 5～7min; (3) because the speed of reactant liquor permeable membrane diafiltration is unanimously poor, repeated relatively poor (otherness of measured result was larger when the protein-chip of same batch of production detected same duplicate samples) that also causes testing result; (4) artificial interference factor is many, be difficult to standardization.
Summary of the invention
The purpose of this utility model provides a kind of protein chip core chamber, and it is uncontrollable to solve in the present percolation protein-chip testing process reaction conditions and course of reaction; Reaction efficiency is lower, and association reaction is abundant not; The artificial interference factor is many, be difficult to standardization, the relatively poor isostructuralism technological deficiency of testing result repeatability.
The technical scheme that the utility model adopts is:
A kind of protein chip core chamber includes box body and upper box cover, it is characterized in that:
Be provided with the absorbent material truss in the described box body, the absorbent material truss is provided with the water-absorption material hopper; The Process window that arranges on the position of water-absorption material hopper and the upper box cover is corresponding;
Absorbent material truss four corner edge have domatic projection, and all the other are the plane;
Absorbent material truss (3) top is provided with the flat driving separated skateboard of U-shaped (2); The initiating terminal of driving separated skateboard direction of propulsion is provided with notch, blocks domatic projection corresponding to position on the absorbent material truss.
Be provided with the promotion button of projection on the described driving separated skateboard, the promotion button window that arranges on position and the upper box cover is corresponding;
Promote button and in promoting the button window, carry out, make driving separated skateboard and absorbent material truss generation relative motion.
The edge of described absorbent material truss is provided with fixed orifice, and the set fixed leg in position and box body edge is corresponding;
The fixed leg of box body inserts the fixed orifice of absorbent material truss, and box body and absorbent material truss are fixed.
Described cartridge inner surface is provided with the buffering spacer of perk.
The bottom land of described water-absorption material hopper is provided with through hole.
The utlity model has following advantage:
The utility model in the reaction time between antigen, antibody or other capture molecules and testing sample on the chip matrix, has improved chip inspection result's repeatability (chip product that is same batch of production significantly reduces for batch interpolation and the difference between batch of a serum sample) by effective control immobilization significantly.Overcome present commercialization percolation protein-chip, because chip matrix and absorbent material contact process is uncontrollable, in testing process, cause the heterogeneity of sample infiltration rate, and the testing result poor repeatability problem that causes and cause disadvantage at the bottom of the detection sensitivity owing to specific immunity association reaction between immobilization antigen, antibody or other capture molecules and the determinand is insufficient.Improve significantly reaction efficiency, significantly improved the repeatability of testing result, increased the detection sensitivity of chip.
The utility model makes chip separate with the absorbent material of lower floor by being located at the indoor driving tripping device of core, when test sample, can and optimize immobilization antigen (antibody) and the reaction time of detected material in the water-absorption material hopper according to the requirement of experiment adjustment, improve reaction efficiency, increase repeatability and the sensitivity of assay.
Description of drawings
Fig. 1 is for can effectively controlling the core chamber outside drawing of reaction time protein chip;
Fig. 2 is for can effectively controlling the structural representation of reaction time protein chip;
Fig. 3 is the structural representation of upper box cover;
Fig. 4 is the structural representation of driving separated skateboard;
Fig. 5 is the structural representation of absorbent material truss;
Fig. 6 is the structural representation of box body.
Among the figure, the 1-upper box cover, 2-driving separated skateboard, 3-absorbent material truss, the 4-box body, the 5-Process window, 6-promotes the button window, the 7-fixed leg, the 8-buffering spacer, 9-promotes button, 10-fixed orifice, 11-water-absorption material hopper.
Embodiment
Below in conjunction with embodiment the utility model is described in detail.
1, structure explanation of the present utility model:
A kind of protein chip core described in the utility model chamber include the upper box cover 1 that box body 4 and box body 4 tops arrange, but the two fastening is fixed.
Be provided with absorbent material truss 3 in the box body 4, four jiaos at the edge of absorbent material truss 3 is provided with four fixed orifices 10, and four jiaos of four the set fixed legs 7 in position and box body 4 edges are corresponding.The fixed leg 7 of box body 4 inserts the fixed orifice 10 of absorbent material truss 3, and box body 4 and absorbent material truss 3 are fixed.The inside surface of box body 4 bottoms is provided with two buffering spacers 8 relative, perk, so that box body 4 communicates with the external world, played the effect of exhaust, be difficult for forming subnormal ambient, can make in addition absorbent material truss 3 directly not contact box body 4 inside surfaces, be easy to install and separate.
The central authorities of absorbent material truss 3 are provided with sagging water-absorption material hopper 11, and the Process window 5 that arranges on the position of water-absorption material hopper 11 and the upper box cover 1 is corresponding, and water-absorption material hopper 11 bottom lands are provided with the through hole that can ventilate.Absorbent material truss 3 four corner edge have domatic projection, and all the other are the plane.Absorbent material truss 3 tops are provided with the flat driving separated skateboard 2 of U-shaped.The initiating terminal of driving separated skateboard 2 direction of propulsion is provided with two notches, blocks two domatic projections corresponding to position on the absorbent material truss 3.
Be provided with the promotion button 9 of projection on the driving separated skateboard 2, the promotion button window 6 of setting is corresponding on position and the upper box cover 1.Promoting button 9 carries out in promoting button window 6, make driving separated skateboard 2 and absorbent material truss 3 that relative motion occur, two domatic projections that are stuck on the absorbent material truss 3 in the notch of driving separated skateboard 2 are released from notch, when driving separated skateboard 2 is advanced to water-absorption material hopper 11 two other domatic projection, driving separated skateboard 2 moves on domatic projection, make between driving separated skateboard 2 and the absorbent material truss 3 and produce the slit, the distance on the vertical direction increases.Because box body 4 and upper box cover 1 are fastenings, so absorbent material truss 3 can be pressed down.Absorbent material truss 3 below correspondences buffering spacer 8, and absorbent material truss 3 is produced anchorage force upwards.
2, assemble method of the present utility model:
During the chip assembling, at first specific protein/polypeptide class antigen, antibody or other capture molecules mode with dot matrix is fixed on the film matrix such as nitrocellulose membrane (NC) in an orderly manner, with tweezers the film chip is fixed in the Process window 5 of upper box cover 1,, film is just facing to the window outside; Then absorbent material truss 3 is placed on four fixed legs 7 in box body 4 bottoms and fix, absorbent material is put into water-absorption material hopper 11, place driving separated skateboard 2 on the absorbent material truss 3 fixing again; Fixing with box body 4 upper box cover 1 at last, can finish the assembling that can effectively control the reaction time protein chip.
3, using method of the present utility model:
During detection, drip cleansing solution profit film by Process window 5 at chip first because chip matrix directly contacts with absorbent material in the water-absorption material hopper 11, by the water absorptivity of absorbent material, cleansing solution in the diafiltration mode naturally through chip.Then advance and promote button 9, promoting button 9 carries out in promoting button window 6, make driving separated skateboard 2 and absorbent material truss 3 that relative motion occur, two domatic projections that are stuck on the absorbent material truss 3 in the notch of driving separated skateboard 2 are released from notch, when driving separated skateboard 2 is advanced to water-absorption material hopper 11 two other domatic projection, driving separated skateboard 2 moves on domatic projection, make between driving separated skateboard 2 and the absorbent material truss 3 and produce the slit, the distance on the vertical direction increases.Because box body 4 and upper box cover 1 are fastenings, so absorbent material truss 3 can be pressed down.At this moment, chip matrix and absorbent material are in released state, sample liquid is added in the Process window 5, makes target antibody, antigen or other target molecules in the sample to be checked have immobilised antigen on time enough and the chip matrix, antibody or other capture molecules that specific immune association reaction occurs.After the reaction time that arrives expection, driving separated skateboard 2 is retracted original position, driving separated skateboard 2 moves down along domatic projection, make that the distance on the vertical direction reduces between driving separated skateboard 2 and the absorbent material truss 3, absorbent material is contacted, the diafiltration of guiding sample liquid permeable membrane with chip matrix.Add at last the cleansing solution washing again and remove not bond, add subsequently developer (immune colloid gold) colour developing, upper machine is read testing result with scanner.When with enzyme labeling, when adding sample to be checked, when dripping enzyme conjugates, also should promote button makes chip matrix separate with absorbent material, allow enzyme conjugates and Ag-Ab immune complex have the sufficient time that specific binding occurs, with the substrate colour developing, read testing result by chip reading instrument again.
The utility model is not limited only to a certain specific immobilization antigen/antibody and other capture molecules to the significantly improvement of protein chip testing result repeatability and sensitivity, be confined to specific thing to be detected, be confined to specific reaction pattern or test method, be confined to specific film chip matrix.But be applicable to various immobilised albumen or polypeptide class antigen/antibody and other capture molecules; Be applicable to differential responses pattern and the test method such as indirect method, double antibody (former) sandwich method, prize law of percolation protein chip; Be applicable to the antigen/antibody bond of collaurum, other tracer molecule marks of HRP; Be applicable to various film chip matrix.The experimenter can according to concrete requirement of experiment flexibly, easily experimental design is adjusted and is optimized.
It is cited that content of the present utility model is not limited to embodiment, and the conversion of any equivalence that those of ordinary skills take technical solutions of the utility model by reading the utility model instructions is claim of the present utility model and contains.
Claims (5)
1. a protein chip core chamber includes box body (4) and upper box cover (1), it is characterized in that:
Be provided with absorbent material truss (3) in the described box body (4), absorbent material truss (3) is provided with water-absorption material hopper (11); The position of water-absorption material hopper (11) is corresponding with the upper Process window (5) that arranges of upper box cover (1);
Absorbent material truss (3) four corner edge have domatic projection, and all the other are the plane;
Absorbent material truss (3) top is provided with the flat driving separated skateboard of U-shaped (2); The initiating terminal of driving separated skateboard (2) direction of propulsion is provided with notch, blocks domatic projection corresponding to the upper position of absorbent material truss (3).
2. a kind of protein chip core according to claim 1 chamber is characterized in that:
Be provided with the promotion button (9) of projection on the described driving separated skateboard (2), the position is corresponding with the upper promotion button window (6) that arranges of upper box cover (1);
Promote button (9) and in promoting button window (6), carry out, make driving separated skateboard (2) and absorbent material truss (3) that relative motion occur.
3. a kind of protein chip core according to claim 1 and 2 chamber is characterized in that:
The edge of described absorbent material truss (3) is provided with fixed orifice (10), and the set fixed leg (7) in position and box body (4) edge is corresponding;
The fixed leg (7) of box body (4) inserts the fixed orifice (10) of absorbent material truss (3), and box body (4) and absorbent material truss (3) are fixed.
4. a kind of protein chip core according to claim 3 chamber is characterized in that:
Described box body (4) inside surface is provided with the buffering spacer (8) of perk.
5. a kind of protein chip core according to claim 4 chamber is characterized in that:
The bottom land of described water-absorption material hopper (11) is provided with through hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220485075 CN202794190U (en) | 2012-09-21 | 2012-09-21 | Chip room of protein chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220485075 CN202794190U (en) | 2012-09-21 | 2012-09-21 | Chip room of protein chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202794190U true CN202794190U (en) | 2013-03-13 |
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ID=47821433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220485075 Expired - Fee Related CN202794190U (en) | 2012-09-21 | 2012-09-21 | Chip room of protein chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202794190U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108593939A (en) * | 2018-07-18 | 2018-09-28 | 广州瑞博奥生物科技有限公司 | A kind of full-automatic protein chip and its application |
-
2012
- 2012-09-21 CN CN 201220485075 patent/CN202794190U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108593939A (en) * | 2018-07-18 | 2018-09-28 | 广州瑞博奥生物科技有限公司 | A kind of full-automatic protein chip and its application |
| CN108593939B (en) * | 2018-07-18 | 2023-11-14 | 瑞博奥(广州)生物科技股份有限公司 | Full-automatic protein chip and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130313 Termination date: 20190921 |