CN202189053U

CN202189053U - Biological chip

Info

Publication number: CN202189053U
Application number: CN2011202952966U
Authority: CN
Inventors: 张灿
Original assignee: Individual
Current assignee: Zhang Can
Priority date: 2011-08-12
Filing date: 2011-08-12
Publication date: 2012-04-11
Anticipated expiration: 2021-08-12

Abstract

The utility model belongs to the technical field of biological detection, and relates to a novel biological chip used for detecting microminiaturized biochemistry, immunity or molecules. The biological chip comprises a frame-type base and a reaction vessel, wherein the reaction vessel comprises a vessel body, a film and a lining; and the lining adopting a frame can be embedded into the vessel; and the film is arranged in the vessel body via the lining. The microtiter plate disclosed by the utility model provides a novel reaction vessel form for single or multiple parallel detection in fields of biochemistry, immunity or molecules.

Description

A kind of biochip

Technical field

The utility model relates to a kind of biochip, and it is applicable to biology, biochemical field, is particularly useful for biochemistry, immunity and Molecular Detection field.

Background technology

In recent years; Along with the development of society and the progress of biomedical technology; The biochemistry that carries out every year, immunity and Molecular Detection quantity are all increasing considerably, and is not only increasingly high to the requirement of reagent reacting cup processing speed, and the demand of personalized fast detecting is also urgent day by day.The reagent reacting cup that is used for full-automatic immune detection equipment in the prior art mostly adopts the structure in townhouse 8 hole * 12 holes; Angle from reagent reacting cup production; Whole plank frame helps the large-scale production reaction cup really; Simultaneously reagent reacting cup quantity can be according to the integral multiple independent assortment of requirement of experiment by 8 holes or 12 holes, and whole plank frame help with laboratory at present common wash the supporting uses of instrument such as plate machine, ELIASA; But when encapsulating reaction cup owing to production is whole board-like; Same plate reagent reacting cup can only encapsulate same probe; Be unfavorable for the requirement of many indexs joint-detection in biomedical the detection, the design feature of whole board-like reagent reacting cup makes the robotization detection almost not have the possibility that realizes simultaneously.This utility model can be widely used in clinical laboratories such as medical and clinical, health and epidemic prevention station, blood station, center and detect first, second, third, hepatitis D, and the medical science of disease aspects such as thyroid gland, reproduction, pregnant parahormone, tumor markers, virus, AIDS, autoimmune disease, parasite detects.

The utility model content

The purpose of the utility model is for solving the problems of the technologies described above; A kind of biochip is provided; The utility model is for the single index of biochemical, immune detection or Molecular Detection or many indexs are parallel and robotization detects brand-new microwell plate pattern and detecting pattern is provided; Can make detection more stable, avoid the mutual pollution problem of sample simultaneously.

A kind of biochip, it comprises frame formula base and reaction cup, it is characterized in that: said reaction cup comprises cup, film and liner; Said liner is a frame, and this frame can be rabbeted the inside at said cup, and said film is arranged in the said cup through said liner.

The utility model is when being used to detect, and the place of its reaction is different from microwell plate, and microwell plate reacts in cup, and the reaction of the utility model is carried out on film; Therefore can realize that many indexs are parallel, detect technique effects such as more stable.

Preferred as technique scheme, said frame formula base and said reaction cup are split-type structural, glass bottom surface of said reaction cup is less than or equal to the rim of a cup face.

Preferred as technique scheme, said reaction cup is a fabricated structure, said cup comprises top body and lower body; Said liner interlocking is in lower body.

Preferred as technique scheme, the bottom of said lower body is provided with fixed body.

Preferred as technique scheme is provided with bayonet arrangement between said lower body and the top body.

Preferred as technique scheme, said reaction cup also comprises film, said film is arranged in the lower body through said liner.

Preferred as technique scheme, said reaction cup also comprises sealing ring, makes said top body and lower body be tightly connected through the sealing ring.

Preferred as technique scheme is outside body socket of said top and the said lower body.

The utlity model has following technique effect:

1, the utility model can with the laboratory conventional wash supporting uses such as plate machine, ELIASA, be particularly useful for biochemistry, immunity and Molecular Detection field;

2, a kind of novel reaction cup of providing of the utility model can carry out independent assortment according to experiment and detections needs, can be single part and detect that also person-portion quantity makes up arbitrarily, the greatly convenient demand of testing and checking;

3, a kind of brand-new reaction compartment pattern and the subsequent treatment pattern that provide of the utility model is used for reaction, detection or other processing procedure in biology, biochemical field;

4, the utility model provides a kind of brand-new biochip; Can carry out independent assortment according to experiment and detection needs; Can be single part and detect that also person-portion quantity makes up arbitrarily, the demand of greatly convenient experiment and check; Help the high-throughout parallel work-flow and the detection of many indexs, multisample, more help the realization of the robotization of plane biochip.

Description of drawings

Fig. 1: the vertical view of 96 hole frame formula bases;

Fig. 2 a: the sectional view of the square reaction cup of integral type;

Fig. 2 b: the sectional view of split type square reaction cup;

Fig. 3: the three-dimensional plot of the square reaction cup lath of bridge-type;

Fig. 4: the three-dimensional plot of square reaction cup liner;

Fig. 5: but the structural representation three-dimensional plot of free modular biochip;

Fig. 6: the structural representation three-dimensional plot that can be used for the biochip of full-automatic chemical luminescence immunoassay.

Embodiment

Below in conjunction with accompanying drawing the utility model is done further explain.

This specific embodiment only is the explanation to the utility model; It is not the restriction to the utility model; Those skilled in the art can make the modification that does not have creative contribution to present embodiment as required after reading this instructions, but as long as in the claim scope of the utility model, all receive the protection of Patent Law.

Embodiment one: but a kind of free modular biochip

But a kind of free modular biochip, this biochip is made up of the film and the liner of array bioprobe reaction cup, point.

Bridge-type coupled reaction cup such as Fig. 2 a, shown in Figure 3, fourth class edge lengths suitable for reading is 7.5mm in it, in the fourth class edge lengths of going to the bottom be 7.3mm, wall thickness is 0.6mm all around, the bottom wall thickness is 1mm, height is 12mm.

Bridge-type coupled reaction cup can 12 is one or 8 one; The reaction cup front indicates arrow upwards shown among Fig. 5 54, with the dot matrix direction of clear and definite biochip.

Shown among Fig. 5 52, be fixed with array bioprobe film and be of a size of 7.2 mm * 7.2mm, the dot matrix scope is 6.0 mm * 6.0mm, at upper left angle point the direction signs point is arranged.

Shown among Fig. 5 51, the suitable for reading outer fourth class edge lengths of liner is 7.4mm, and the outer fourth class edge lengths of end opening is 7.3mm, highly is 5mm, and wall thickness is 0.2mm all around.

Above reaction cup material is a polystyrene, rigid, milky, and interior village material is PVC, and necessarily soft, milky are arranged.Reaction cup and liner are once injection molded integral structure.

But the assembling of free modular biochip is as shown in Figure 5; With tweezers at the bottom of being fixed with array bioprobe film 52 and putting into bridge-type coupled reaction cup; Put into the certain soft liner 51 of tool again, with push-tight is with compacting stationary probe film at the bottom of the past reaction cup of tweezers, this can the free modular biochip.

But but this free modular biochip mating edge frame base in the process that detects uses shown in Fig. 1 a.

Embodiment two: a kind of oncoprotein chip that can be used for the full-automatic chemical luminescence immunoassay

A kind of oncoprotein chip that can be used for the chemiluminescence automatic immunity, this oncoprotein chip is made up of the PDMS film and the liner of array oncoprotein probe combined type reaction cup, point.

The combined type reaction cup is divided into top body 24 and lower body 26 2 parts shown in Fig. 2 b.The beneath face of lower body 26 is useful on insertion or the fixed body 27 of embedding of the match stick nose shape of fixed biologically chip.The lower end wall 28 of top body 24 is an inwall breach type, and wall thickness is 0.7mm, and breach height in lower end is 4mm; The upper end wall thickness is 1.4mm.There is inner groovy 25 position, upper middle of lower body 26; The groove radius is 0.3mm, and the indent at four angles of groove is the circular arc chamfering structure so that keep sealing when putting into O-ring seal, and the inside and outside height of the wall of lower body is 5.05mm; Four limit wall thickness are 0.7mm, and the diapire of lower body 26 is thick to be 1.4mm.(not comprising lower body fixed body 27)

Inwall four limits of combined type reaction cup equate all that up and down be 7.5mm, the inwall height is 15mm.

The diameter of section of the O-ring seal of combined type reaction cup is 0.4; The outer fourth class edge lengths of the wall suitable for reading of liner is 7.5mm, and the outer fourth class edge lengths of end opening wall is 7.4mm, highly is 5mm, and wall thickness is 0.2mm all around.

The reaction cup material is a polystyrene, rigid, black, and inner lining material is PVC, and necessarily soft, black are arranged.Reaction cup and liner are once injection molded integral structure.

Surface grafting and carboxylated PDMS film thickness are 2.0mm.

Fixing oncoprotein probe array on the PDMS film:

The preparation of PDMS film activating solution: with deionized water preparation 0.289g/15ml EDC solution, 0.326g/15ml NHS solution.

The activation of PDMS film: surface grafting and carboxylated PDMS film are soaked into by concussion activation in the EDC-NHS solution of 1:1 (V/V) mixing 30 minutes; Use deionized water drip washing then three times; Put on the PDMS film after the activation through the micro-sampling technology with point sample instrument immediately after drying up under the nitrogen; The point sample volume is 10nl, and following antibody is by two points of every kind of antibody point (double point up and down), and dot matrix is 6 * 6 (totally 36 points); Two points wherein: one is low Quality Control point, and another is high Quality Control point; The array area scope is 6.2 mm * 6.2mm, at the upper left angle point of each array the direction signs point is arranged, and the array pitch of every chip block is from matching with special cutting die.

Point sample antibody (first antibody): CA199, AFP, CEA, CA242, CA153, NSE, CA125, HCG, CA724, C-PSA, f-PSA, SCC, CK19, CYFRA21-1, ProGRP, β 2-MG, 17 kinds of indexs of TPA.

The PDMS film that point sample finishes is put into sealing bag, spend the night; Then with the confining liquid sealing that contains 10% (W/V) sweet mellow wine and 0.1M PH7.2PB damping fluid 30 minutes; After sealing finishes; Through stationary temperature 20 degree, humidity 30% dry 3 hours, handling the back, to become every (being fixed with the oncoprotein array) diaphragm with special cutting die crush-cutting be 7.4 mm * 7.4mm.

The chip assembling: the oncoprotein chip assembling that can be used for the chemiluminescence automatic immunity is like Fig. 6; Be inserted in O-ring seal the groove of lower body 65; Put into 65 ends of reaction cup lower body (film faces up) with tweezers being fixed with tumour m protein antibody array formula probe PDMS film 63; Put into the certain soft liner 62 of tool again; Push-tight is with compacting stationary probe film at the bottom of the reaction cup with tweezers, and there is the top body 61 of arrow logo and consistent with the array direction on the PDMS diaphragm in the front that is locked, promptly accomplishes the assembling that this is this oncoprotein chip.

Detect and analyze:

The oncoprotein chip is inserted on the rotating disc type chip firm banking of automatic biological chip machine, (below be operating as robotization) adds sample 100ul in the reaction cup, and the concussion incubation is 20 minutes in 37 degree thermostats, blots liquid in the hole.In sample cup, add ELISA washing lotion flushing 1 minute (washing the plate machine with ELISA, to clean principle the same: the hour hand primary drying is washed, the imbibition of minute hand head), blot liquid in the reaction cup; In reaction cup, add 100ul enzyme mark working fluid again, 37 degree concussion incubations 20 minutes blot liquid in the reaction cup; In sample cup, add ELISA washing lotion flushing 1 minute, blot liquid in the reaction cup; Pull out the top body of reaction cup; Mixed liquor to (being array central authorities) adding 8ul luminescent solution A of probe PDMS film central authorities and the luminous B of 8ul slightly shakes mixing, and room temperature left standstill 1 minute; Take pictures by the CCD reading apparatus, by analysis software the dot matrix light signal is analyzed at last and provided the result.

Claims

1. biochip, it comprises frame formula base and reaction cup, it is characterized in that: said reaction cup comprises cup, film and liner; Said liner is a frame, and this frame can be rabbeted the inside at said cup, and said film is arranged in the said cup through said liner.

2. a kind of biochip according to claim 1 is characterized in that: said frame formula base and said reaction cup are split-type structural, and the cup bottom surface of said reaction cup is less than or equal to the rim of a cup face.

3. a kind of biochip according to claim 1 is characterized in that: said reaction cup is a fabricated structure, and said cup comprises top body and lower body; Said liner interlocking is in lower body.

4. a kind of biochip according to claim 3 is characterized in that: the bottom of said lower body is provided with fixed body.

5. a kind of biochip according to claim 3 is characterized in that: be provided with bayonet arrangement between said lower body and the top body.

6. a kind of biochip according to claim 3 is characterized in that: said reaction cup also comprises sealing ring, makes said top body and lower body be tightly connected through the sealing ring.

7. a kind of biochip according to claim 5 is characterized in that: outside body socket of said top and the said lower body.