CN100504386C - Miniature reaction cup type protein chip - Google Patents
Miniature reaction cup type protein chip Download PDFInfo
- Publication number
- CN100504386C CN100504386C CNB031419968A CN03141996A CN100504386C CN 100504386 C CN100504386 C CN 100504386C CN B031419968 A CNB031419968 A CN B031419968A CN 03141996 A CN03141996 A CN 03141996A CN 100504386 C CN100504386 C CN 100504386C
- Authority
- CN
- China
- Prior art keywords
- reaction
- protein chip
- protein
- cup
- chip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention relates to a miniature reaction cap type protein chip and its preparation method. Said miniature reaction cap type protein chip is a solid phase carrier which is made up by adopting organic polymer material and is equipped with cavity chamber structure. All the cavity chambers aremutually separated, and regularly arranged, and can be independently used, and are mutually connected between them by means of breakable bridge type structure. The adoption of said invented protein chip can make multiindex parallel detection of protein sample, can make the detection more stable, at the same time it has no mutual pollution problem of samples.
Description
Technical field:
The invention belongs to biological technical field, be specifically related to cup type protein chip of a kind of minisize reaction and preparation method.
Background technology:
Biochip technology is an emerging biometric technology that rises middle nineteen nineties, because of it can carry out the parallel analysis of multiple information in primary first-order equation, and is subjected to attracting attention of numerous researchers, is applied in many field of biology rapidly.Biochip technology is pressed the detected object classification, mainly is divided into genetic chip and protein chip two classes.Protein chip is a kind of technology of forward position.The microarray that it constitutes by the multiple proteins that is fixed on the supporting dielectric, directly detect the method for the existing detection urine of specific function material (significant albumen), serum and other body fluid, as ELISA, labelled with radioisotope, golden mark, fluorescence, chemiluminescence, time-resolved fluorescence etc., be that single index detects mostly, quantity of information is few, and all have limitation, as markers step complexity, instrument and reagent costliness, operation is loaded down with trivial details, sensitivity is low, easy pollution etc.Compare with the traditional detection mode, protein chip technology has the following advantages:
1) high flux can carry out the detection of many person-portions simultaneously, also can use separately.
2) high sensitive and specificity;
3) operation is quick, easy, is applicable to clinical instant detection, only need just can finish whole detection reaction process in one and a half hours;
4) required sample size is few.
But the prior biological chip all is fixed on multiple protein on the one plane solid-phase matrix, and the material of employing has slide, silicon chip, nylon membrane, nitrocellulose filter etc.Prepare the protein chip needs with inorganic material such as slide and silicon chips this carrier is carried out chemical modification, complicated operation, and also the degree of uniformity of chemical modification has a strong impact on protecpectic thereafter quality.Prepare protein chip with membrane type organic materials such as nylon membrane and nitrocellulose filters, though do not need to carry out chemical modification, operate simply relatively, because there is the capillary percolation phenomenon in it, its homogeneity can not be guaranteed simultaneously, influences testing result unavoidably.
Summary of the invention:
Technical matters to be solved by this invention is to provide the minisize reaction that a kind of detection is more stable, n.s. pollutes mutually cup type protein chip.
The cup type protein chip of minisize reaction disclosed by the invention is a kind of solid phase carrier with chamber structure that adopts organic polymer material to make, and each chamber is separated arrangement regularly mutually, and can independently use, and has broken bridge architecture to link each other.
The reaction glass stand that the cup type protein chip of minisize reaction disclosed by the invention is made by supporting with it organic polymer material supports.
The cup type protein chip of minisize reaction disclosed by the invention, wherein said chamber can be a different shape, comprises right cylinder, rectangular parallelepiped, prism, cone or pyramid etc.
Organic polymer material of the present invention comprises that poly-third hands over fat (PLA), poly-fat (PGA), poly-own lactones (PCL), polymethyl methacrylate (PMMA), ABS resin, polystyrene (PS), cellulose, cellulose one tygon, poly-hydracrylic acid fat or the polypropylene etc. handed over.
Be further described below in conjunction with the structure of accompanying drawing the cup type protein chip of minisize reaction of the present invention.
Fig. 1 is the structural representation of the cup type protein chip of minisize reaction.Wherein Fig. 1-a is a vertical view, and Fig. 1-b is the A-A cut-open view.Minisize reaction cup upper/lower terminal is recessed to form upper and lower two chambers as seen from Figure 1, is respectively chamber 1 and chamber 3.The bottom surface 2 of chamber 1 is as the protein chip carrier, and various albumen are fixed on 2 in an orderly manner, and biochemical reaction will carry out in chamber 1, and it is stable that the chamber 3 of formation is placed reaction cup, is easy to stand." ear " formula structure 4, handled easily are arranged at each " reaction cup " top.Have " bridge-type " structure 5 to link between " reaction cup ", this " bridge-type " structure is broken.Each " reaction cup " has complete separated independent reaction space, can independently use.The projection 6 of holding up is arranged on each reaction cup, and this projection is corresponding with the reaction glass stand.
Fig. 2 is and the matching used reaction glass stand of the cup type protein chip of minisize reaction synoptic diagram that this reaction glass stand the person of being convenient to operation operate a plurality of reaction cup simultaneously.The reaction glass stand is the plate of porous, and the shape in hole is corresponding with reaction cup, has several to dozens of holes on it and does not wait, and fluted 9 in every hole, this groove is corresponding with reaction cup projection 6, reaction cup can be fixed in glass stand.Numeric character 8 and alphabetical mark 7 are arranged on the glass stand, and the handled easily personnel carry out record, distinguish albumen sample to be measured.
The cup type protein chip of minisize reaction of the present invention can be made organic polymer material injection moulding, mold pressing or punching press, and this polymeric material does not have the capillary percolation phenomenon, firmly and have toughness, compares with the plane formula protein chip that other materials is made and has the following advantages:
1) from truly having solved the problem that interferes with each other between reaction chamber.The conventional way of protein chip is to fix a plurality of protein array big opening on nitrocellulose filter, glass matrix or other matrix at present.Adopt the way of cutting or sealing that dot matrix is separated then, avoid the phase mutual interference.But the method can not guarantee absolute effectively, cause sample to pollute mutually probably.The protein chip that reacts cup type fully independently is fixed on each dot matrix in the different reaction cup from the beginning, thereby has solved the mutual pollution problems of sample at all.
2) make on the matrix protecpectic amount more accurate.Membrane type matrix has water absorptivity, and therefore, factors such as the capillary percolation time of liquid, protein adsorption ability will have a strong impact on final fixing protein content.Owing to membrane type matrix preparation technology and material characteristic, there is certain problem in its homogeneity, may cause in the different proteins dot matrix simultaneously, and protecpectic amount difference influences testing result.React cup type protein chip and do not have water absorptivity, can guarantee in the different proteins dot matrix, stationary phase protein content together guarantees the stability that detects.
The protein example that this cup type protein chip of minisize reaction is fixed can be antigen, antibody, acceptor, particularly with body in the albumen of disease marker protein specific bond, as SARS virus associated protein, autoimmune disease associated protein, tumor correlated albumen etc.Owing to fixed above-mentioned protein sample in order in each " reaction cup ", therefore can reach the purpose of various product, the parallel detection of many indexs.
The method that adopts the cup type protein chip of minisize reaction of the present invention to prepare the protein chip of many indexs parallel detection comprises:
1) with dilution the protein of main points system on the protein chip is diluted to finite concentration; With the automatic point sample of chip system with these protein spots in reaction cup, point sample density is at 10-200 point/cm
2, point sample concentration is 0.01-10mg/ml, point sample amount 0.1-40nl/ point.
2) with confining liquid protein chip sealing is handled, adsorbed other protein at random, influence reaction result to avoid on the solid phase carrier not the zone of ankyrin.Dry 0.5-24 hour.
3) the protein chip freeze drying is handled 2-8 ℃ of storages.For the long-term protein active that keeps, adopt the method for freeze-drying to increase product stability.
Unless refering in particular to, following concentration unit is w/v.
Wherein said dilution prescription is:
0-0.04mol/l PBS+0-0.2%BSA, the CBS solution of 0-0.04mol/l TE+0-0.2%BSA or 0--80mM or 0-40mM Tris.HCL+0-80mM NaCl+0--5mM EDTA or 0--50mM NaAc or pH are the PBS (KH of 7.0-8.0
2PO
4-Na
2PO
4).
Wherein said confining liquid prescription is: 70-95%CBS+5-15% calf serum+1-9% sucrose.Or 1-9%BSA, 1-9% sucrose, 0.1-1%NaN
3PBS.
Adopting above-mentioned protein chip is main body, also can be used for the kit of many indexs parallel detection, utilizes chemiluminescence reaction to detect the content of multiple proteins in body fluid simultaneously.This kit comprises:
1, protein chip is fixed with the albumen of disease marker protein specific bond in multiple and the body on it, as kinds of tumors mark antibody, and the property exempted from disease antigen certainly, SARS proteantigen
2, a kind of formulated with the finite concentration proportioning, and the protein of mark peroxidase or polyprotein matter mixed liquor.As the anti-kinds of tumors mark mixtures of antibodies of horseradish peroxidase-labeled, the goat anti-human igg of horseradish peroxidase-labeled, rabbit anti-human igg or mouse-anti human IgG etc.;
3, a kind of Chemical composition that produces the compound composition of chemiluminescence reaction;
4, a kind of cleansing solution of protein chip;
5, standard items: a kind of standard mixed liquor of known protein matter, this component are not necessary, and it is different and select for use to look detected property of protein.
Description of drawings:
The cup type protein chip structural representation of Fig. 1 minisize reaction of the present invention
1-a reaction cup vertical view
The 1-bA-A cut-open view
Fig. 2 reacts the glass stand synoptic diagram;
Fig. 3 synoptic diagram of patient's SARS blood sample result of protein chip detection of the present invention;
Fig. 4 synoptic diagram of the dry syndrome patient blood sample result of protein chip detection of the present invention
Fig. 5 synoptic diagram of the rheumatoid arthritis human blood sample result of protein chip detection of the present invention
Fig. 6 synoptic diagram of the hepatocarcinoma patient blood sample result of protein chip detection of the present invention
Further set forth the present invention below in conjunction with specific embodiment, but do not limit the scope of the invention.
Embodiment:
Embodiment 1: be used to detect the preparation of SARS antibody protein chip
Reaction cup is by polystyrene (PS) injection mo(u)lding, and the minisize reaction cup-shaped is a right cylinder, and the reaction glass stand is by the ABS resin injection mo(u)lding.
1) the S albumen with SARS arrives 0.04mg/ml to 0.04mg/ml, standard I gG albumen with diluted with diluted to 0.05mg/ml, 3CL albumen with diluted to 0.06mg/ml, M albumen with diluted to 0.05mg/ml, N albumen with diluted; With the automatic point sample of chip system with these protein spots in reaction cup, every kind of 2 in albumen is parallel, point sample density is at 10-200 point/cmm
2, point sample amount 40nl/ point.Dot matrix is arranged as follows:
| A | B | C | D | E | |
| 1 | M | 3CL | GST | Standard I gG | |
| 2 | M | 3CL | GST | Standard I |
|
| 3 | S1 | S2 | S3 | S4 | |
| 4 | S1 | S2 | S3 | S4 | N |
2) with confining liquid protein chip sealing is handled, adsorbed other protein at random, influence reaction result to avoid on the solid phase carrier not the zone of ankyrin.Dry 30-60 minutes.
3) the protein chip freeze drying is handled 2-8 ℃ of storages.For the long-term protein active that keeps, adopt the method for freeze-drying to increase product stability.
Unless refering in particular to, following concentration unit is w/v.
Wherein said dilution prescription is: 0.01mol/l PBS+0.2%BSA or 0.02mol/lTE+0.2%BSA
Wherein said confining liquid prescription is: 75%CBS+8% calf serum+7% sucrose.
Detect the SARS patients serum with above-mentioned protein chip, wherein anti-S protein antibodies, anti-M protein antibodies, anti-N protein antibodies, anti-3CL protein antibodies is all positive, and the result is as shown in Figure 3.
Embodiment 2: be used to detect the preparation of autoimmune disease protein chip
Reaction cup is by polystyrene (PS) injection mo(u)lding, and the minisize reaction cup-shaped is a rectangular parallelepiped, and the reaction glass stand is by the ABS resin injection mo(u)lding.
1) dsDNA albumen is arrived 0.3mg/ml with diluted, SSA-52 albumen arrives 0.05mg/ml with diluted, U1RNP albumen arrives 0.05mg/ml with diluted, SSB albumen arrives 0.1mg/ml with diluted, Jo-1 albumen arrives 0.1mg/ml with diluted, CK19 albumen arrives 0.1mg/ml with diluted, Scl-70 albumen arrives 0.05mg/ml with diluted, SSA-60 albumen arrives 0.1mg/ml with diluted, Sm albumen arrives 0.07mg/ml with diluted, Histone albumen arrives 0.1mg/ml with diluted, ssDNA albumen arrives 0.1mg/ml with diluted, RF albumen arrives 0.1mg/ml with diluted, CENP-B albumen arrives 0.1mg/ml with diluted, CCP albumen arrives 0.05mg/ml with diluted; With the automatic point sample of chip system with these protein spots in reaction cup, every kind of 2 in albumen is parallel, point sample density is at 40 points/cm
2, point sample amount 20nl/ point.
Dot matrix is arranged as follows:
| A | B | C | D | E | F | |
||
| 1 | ANA | Control | IgG1 | IgG2 | IgG3 | IgG4 | IgG5 | |
| 2 | ANA | Control | IgG1 | IgG2 | IgG3 | IgG4 | IgG5 | |
| 3 | SSA-60 | Sm | Histones | ssDNA | G-IgG | CENP-B | CCP | |
| 4 | SSA-69 | Sm | Histones | ssDNA | G-IgG | CENP- | CCP | |
| 5 | dsDNA | SSA-52 | uRNP | SSB | Jo-1 | CK-19 | Scl-70 | |
| 6 | dsDNA | SSA-52 | uRNP | SSB | Jo-1 | CK-19 | Scl-70 |
2) with confining liquid protein chip sealing is handled, adsorbed other protein at random, influence reaction result to avoid on the solid phase carrier not the zone of ankyrin.Dry 30-60 minutes.
3) the protein chip freeze drying is handled 2-8 ℃ of storages.
Unless refering in particular to, following concentration unit is W/V.
Wherein said dilution prescription is: 0.01mol/l PBS+0.2%BSA or 0.02mol/lTE+0.2%BSA
Wherein said confining liquid prescription is: 80%CBS+10% calf serum+5% sucrose.
Detect the dry syndrome patients serum with above-mentioned protein chip, ANA wherein, anti-SSA-60 antibody, anti-SSA-52 antibody, anti-Histones antibody, anti-SSB antibody is all positive, and the result is as shown in Figure 4.
Embodiment 3: based on the using method of the detection kit that is used to detect the autoimmune disease protein chip
Reagent constituents: protein chip; Reactant liquor; Detect liquid; Cleansing solution.
1,10 times of distilled water dilutings of concentrated cleaning solution.
2, serum is pressed the 1:50 dilution with cleansing solution, draws 200 μ l and adds in the reacting hole.
3,37 ℃ of incubations vibrated 30 minutes.Discard the liquid in the hole.
4, fill it up with cleansing solution after the dilution in the hole, incubation vibration 5 minutes discards cleansing solution.Wash altogether 3 times.
5, add 200 μ l reactant liquors in every reaction cup.
6, with step 3.
7, with step 4.
8, in each reaction cup, add 150 μ l and mixed 15 minutes detection liquid A and B mixed liquor, left standstill 60 seconds.
9, with the line data analysis of going forward side by side of HD-2001 series of biologic chip detector reading of data.
With above-mentioned protein chip detection type rheumatic arthritis patients serum, wherein anti-G-IgG (rheumatoid factor) antibody, anti-CK-19 antibody is all positive, and the result is as shown in Figure 5.
Embodiment 4: be used to detect tumor marker protein chip production method and kit
Reaction cup is by polystyrene (PS) injection mo(u)lding, and the minisize reaction cup-shaped is a right cylinder, and the reaction glass stand is by the ABS resin injection mo(u)lding.
1) will resist AFP antibody to arrive 0.25mg/ml with diluted, to resist CEA antibody to arrive 0.5mg/ml with diluted, to resist NSE antibody to arrive 0.3mg/ml with diluted, to resist CA12-5 antibody to arrive 0.5mg/ml with diluted, to resist CA153 antibody to arrive 0.5mg/ml with diluted, to resist CA199 antibody to arrive 0.5mg/ml with diluted, to resist B-HCG antibody to arrive 0.7mg/ml with diluted, to resist HGH antibody to arrive 0.3mg/ml with diluted, to resist CA242 antibody to arrive 0.7mg/ml with diluted, to resist PSA antibody to arrive 0.25mg/ml with diluted, to resist f-PSA antibody to arrive 0.3mg/ml with diluted, with the automatic point sample of chip system with these protein spots in reaction cup, every kind of 2 in albumen is parallel, point sample density is at 40 points/cm
2, point sample amount 20nl/ point.Dot matrix is distributed as
| A | B | C | | E | F | ||
| 1 | HGH | HGH | CA-242 | CA-242 | | Ferritin | |
| 2 | CA19-9 | CA19-9 | AFP | AFP | CA15-3 | CA15-3 | |
| 3 | f-PSA | f-PSA | NSE | | CEA | CEA | |
| 4 | PSA | PSA | CA-125 | CA-125 | b-HCG | b-HCG |
2) with confining liquid protein chip sealing is handled, adsorbed other protein at random, influence reaction result to avoid on the solid phase carrier not the zone of ankyrin.Dry 60 minutes.
3) the protein chip freeze drying is handled 2-8 ℃ of storages.
Unless refering in particular to, following concentration unit is W/V.
Wherein said dilution prescription is: 0.01mol/l PBS+0.2%BSA or 0.02mol/l TE+0.2%BSA
Wherein said confining liquid prescription is: 80%CBS+10% calf serum+5% sucrose.
Prepare the kit that is used for in-vivo tumour mark content as main body with above-mentioned protein chip.Reagent constituents comprises protein chip; Reactant liquor; Detect liquid; Cleansing solution; Standard items (dried frozen aquatic productses of a series of known protein concentration tumor markerses).
With the content of tumor markers in the serum of this kit detection liver cancer patient, wherein AFP and CA-125 are all positive, and the result as shown in Figure 6.
Claims (6)
1, the cup type protein chip of a kind of minisize reaction, it is characterized in that this chip is the solid phase carrier with chamber structure that adopts organic polymer material to make, each chamber is separated arrangement regularly mutually, and can independently use, and has broken bridge architecture to link each other;
Wherein, minisize reaction cup upper/lower terminal is recessed to form upper and lower two chambers, be respectively chamber (1) and chamber (3), the bottom surface (2) of chamber (1) is as the protein chip carrier, " ear " formula structure (4) is arranged at each reaction cup top, has bridge architecture (5) to link between reaction cup, and this " bridge-type " structure is broken, the projection (6) of holding up is arranged on each reaction cup, corresponding with the reaction glass stand; Described reaction glass stand is the plate of porous, and the shape in hole is corresponding with reaction cup, have several to dozens of holes on it and do not wait, fluted in every hole (9), this groove is corresponding with reaction cup projection (6), and numeric character (8) and alphabetical mark (7) are arranged on the glass stand.
2, the cup type protein chip of minisize reaction according to claim 1 is characterized in that wherein said chamber shape is right cylinder, rectangular parallelepiped, prism, cone or pyramid.
3, the cup type protein chip of minisize reaction according to claim 1 is characterized in that wherein said organic polymer material is the poly-third friendship fat, poly-fat, poly-own lactones, polymethyl methacrylate, ABS resin, polystyrene, cellulose, cellulose-tygon, poly-hydracrylic acid fat or the polypropylene handed over.
4, the cup type protein chip of minisize reaction according to claim 1 is used, and it is characterized in that described protein chip is used to prepare the device of many indexs parallel detection of the albumen of disease marker protein specific bond in the body.
5, the cup type methods of making protein chips of the described minisize reaction of a kind of claim 1 is characterized in that this method may further comprise the steps:
1) organic polymer material injection moulding, mold pressing or punching press are made the cup type protein chip of minisize reaction;
2) with dilution the protein of main points system on the protein chip is diluted to finite concentration; With the automatic point sample of chip system with these protein spots on the bottom surface (2) of reaction cup chamber (1), point sample density is at 10-200 points/cm
2, point sample concentration is 0.01-10mg/ml, point sample amount 0.1-40nl/ point;
3) with confining liquid the protein chip sealing is handled dry 0.5-24 hour;
4) the protein chip freeze drying is handled 2-8 ℃ of storages.
6, the cup type methods of making protein chips of minisize reaction according to claim 5, it is characterized in that wherein said dilution prescription is w/v:0-0.04mol/l PBS+0-0.2%BSA, the CBS solution of 0-0.04mol/l TE+0-0.2%BSA or 0-80mM or 0-40mM Tris.HCL+0-80mM NaCl+0-5mM EDTA or 0-50mMNaAc or pH are the PBS of 7.0-8.0; Wherein said confining liquid prescription is w/v:70-95%CBS+5-15% calf serum+1-9% sucrose, or 1-9%BSA, 1-9% sucrose, 0.1-1%NaN
3PBS.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031419968A CN100504386C (en) | 2003-07-31 | 2003-07-31 | Miniature reaction cup type protein chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031419968A CN100504386C (en) | 2003-07-31 | 2003-07-31 | Miniature reaction cup type protein chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1580770A CN1580770A (en) | 2005-02-16 |
| CN100504386C true CN100504386C (en) | 2009-06-24 |
Family
ID=34579318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031419968A Expired - Fee Related CN100504386C (en) | 2003-07-31 | 2003-07-31 | Miniature reaction cup type protein chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100504386C (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101126759B (en) * | 2007-09-21 | 2011-05-11 | 王琳 | Plastic test tube protein chip, its preparation method and uses |
| CN101750506A (en) * | 2008-12-12 | 2010-06-23 | 北京量质科技有限公司 | Reagent reacting cup and fully automatic inspection and detection instrument |
| CN102043043A (en) * | 2010-12-10 | 2011-05-04 | 张会生 | Multi-project biochemical test analysis integrated kit and method thereof |
| CN102530829B (en) * | 2010-12-16 | 2014-11-05 | 上海交通大学附属第一人民医院 | Method for preparing micro-fluidic chip by freeze drying process |
| CN103105489B (en) * | 2013-01-23 | 2014-12-24 | 深圳市亚辉龙生物科技有限公司 | Western blot kit for detecting antibody of autoimmune disease and preparation method thereof |
| CN108646040A (en) * | 2018-07-03 | 2018-10-12 | 无锡国盛生物工程有限公司 | Full-automatic protein chip immunity analysis instrument control system and method |
| CN108593939B (en) * | 2018-07-18 | 2023-11-14 | 瑞博奥(广州)生物科技股份有限公司 | Full-automatic protein chip and application thereof |
| CN109745934B (en) * | 2019-03-18 | 2023-11-21 | 中国人民解放军军事科学院军事医学研究院 | Array type synthesizer and inkjet synthesizer |
-
2003
- 2003-07-31 CN CNB031419968A patent/CN100504386C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1580770A (en) | 2005-02-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104718454B (en) | Possess lateral flow assay strip and the lateral flow assay test kit for this strip of auxiliary mat | |
| CN101821620B (en) | Microfluidic sensor complex structure | |
| Cheng et al. | based ELISA | |
| US3482943A (en) | Reagent deposition device | |
| CN102076415B (en) | Liquid-transfer device particularly useful as a capturing device in a biological assay process | |
| Zheng et al. | High-throughput immunoassay through in-channel microfluidic patterning | |
| US20200271643A1 (en) | Methods and systems for the detection of analyte molecules | |
| JP2011017707A (en) | Biochip for archiving and medical laboratory analysis of biological sample material | |
| KR20180031612A (en) | A Method of Rapid Diagnosis With High Sensitivity By Using Single Diagnosis Chip Comprising Reaction and Analysis Process | |
| JP2013076713A (en) | Manufacturing method of aggregation reagent, aggregation reagent or product material produced thereby, and analysis object measuring method with the same, and test kit and analysis device | |
| CN100504386C (en) | Miniature reaction cup type protein chip | |
| EP2023139B1 (en) | Immunoassay method and chip | |
| JPH09159673A (en) | Microplate | |
| US20110318822A1 (en) | Analytical strip | |
| KR102228913B1 (en) | Microfluidic device and test apparatus | |
| CN106153891A9 (en) | Three dimensional biological marker detection device, preparation method and the method for detecting biomarker | |
| CN103197071A (en) | Preparation and application of human apoptosis factor quantitative antibody chip | |
| JPH1019895A (en) | Micro plate | |
| US20180246088A1 (en) | Multi-unit for conducting biochemical test and immunological test and testing method thereof | |
| US8304255B1 (en) | Immunoassay cuvettes | |
| US9005951B2 (en) | Method and biochip for studying a chemical sample | |
| CN110261592B (en) | Qualitative and quantitative determination method for analyte | |
| CN103439493A (en) | Aptamer percolated biochip and preparation method thereof | |
| Matson | Well-based antibody arrays | |
| CN101236198A (en) | Protein chip for antibody typing, method for making same and its applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: GLOBAL NETWORK (PANAMA) S.A. Free format text: FORMER OWNER: QIAN YING Effective date: 20101101 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 361009 GROUP 3, XIGUO VILLAGE, KAIYUAN DISTRICT, XIAMEN CITY, FUJIAN PROVINCE TO: PANAMA CITY, PANAMA |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20101104 Address after: 313000 Zhejiang province Huzhou Tianheng Road No. 666 Patentee after: Huzhou Shukang Biological Technology Co., Ltd. Address before: 200233, No. 51 North Qinzhou Road, building 4, building 1089, Shanghai Patentee before: Shukang Bio-Technology Co., Ltd., Shanghai |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090624 Termination date: 20200731 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |