CN103105489B - Western blot kit for detecting antibody of autoimmune disease and preparation method thereof - Google Patents
Western blot kit for detecting antibody of autoimmune disease and preparation method thereof Download PDFInfo
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Abstract
The invention provides a western blot kit for detecting the antibody of autoimmune disease and a preparation method of the western blot kit, and relates to a western blot kit for detecting related antibodies of various autoimmune diseases, aiming at overcoming the technical defect that a western blot product is unavailable for testing and screening various autoimmune diseases in the prior art. The nitrocellulose membrane or the nylon membrane contains at least two parallel detection lines coated by at least two of ten natural antigens or recombinant antigens, i.e. dsDNA (deoxyribonucleic acid), Sm/RNP (ribonucleoprotein), CCP (critical compression pressure), SSA (sulfosalicylic acid), SSB (single-strand binding protein), GAD (glutamic acid decarboxylase), ICA (islet cell antibody), IA-2A (islet cell), TG (triglyceride) and AMA-M2 (anti-mitochondrial antibody), a high-concentration quality control band, a median-concentration quality control band and a low-concentration quality control band. The deficiency of the detection sensitivity and the specificity of the single autoantibody can be overcome, the operating complexity for independently detecting the related autoantibody of various diseases one by one can be overcome, and the detection efficiency and the result judging accuracy degree can be greatly improved.
Description
Technical field
The present invention relates to a kind of HP immunoblotting kit detecting various autoimmune disease relevant antibodies, also relate to preparation method and the using method of this HP immunoblotting kit, belong to biomedical sector.
Background technology
Autoimmune disease (autoimmune diseases) refers to that body causes the disease caused by damaged self tissue to autoantigen generation immune response, WHO(World Health Organization, the World Health Organization (WHO)) once pointed out that its number of patients was accounting for total crowd's 3 ~ 5%, according to authority's statistics, the incidence of disease of China autoimmune disease patient is more than 6,000 ten thousand people.Autoimmune disease is apt to occur in women and the elderly, and be characterized in that onset is slow, the course of disease is grown and recurrent exerbation, the middle and advanced stage of disease is very large to health hazard, the quality of life of patient is low, weak curative effect, and early diagnosis contributes to the clinic control of autoimmune disease.
Many autoantibodies just can detect in premorbid a period of time of relevant disease from serum.In decades, immunological investigation has been found that in most autoimmune disease patient's serum and there is specific autoantibody, the anti-Sm antibody of such as systemic loupus erythematosus and Anti-hCG action, the antiCCP antibody of rheumatoid arthritis, the anti-SSA antibody of Sjogren syndrome and anti-SSB antibody, the anti-GAD antibody of insulin-dependent diabetes mellitus (IDDM), anti-ICA antibody and anti-IA-2A antibody, the anti-TG antibody of autoimmune thyroid disease, the anti-AMA-M2 antibody of primary biliary cirrhosis of liver, many specific autoantibodies are ingredients important in autoimmune disease diagnostic criteria.
Systemic loupus erythematosus, rheumatoid arthritis, Sjogren syndrome, insulin-dependent diabetes mellitus (IDDM), autoimmune thyroid disease, primary biliary cirrhosis of liver etc. are common autoimmune diseases, the feature of its clinical manifestation is often ignored by patient, therefore, need regularly to check UP examination.At present, domestic and international clinical examination field has the Western blotting product that minority detects for single autoimmune disease associated antibodies, but there is no the Western blotting product for various autoimmune disease health check-up examination.Existing western blot reagent adopts SDS-PAGE technology to transfer on blotting membrane by antigen by molecular size range substantially, the conformation of many antigenic determinants may be destroyed in the process, can not occur to combine or non-specific binding occurs with the specific antibody in clinical sample, cause result misjudgment.Further, existing product system of quality control imperfection, is not easy to operator and carries out quick, intuitive and accurate judgement to colour developing result.
Summary of the invention
In sum, the object of the invention is to the Western blotting product for there is no at present for various autoimmune disease health check-up examination, and existing western blot reagent exists above-mentioned deficiency, and a kind of of proposition detects HP immunoblotting kit of autoimmune disease antibody and preparation method thereof.
For realizing object of the present invention, adopt following technical scheme:
Detect the HP immunoblotting kit of autoimmune disease antibody, comprise: reaction film bar, enzyme conjugates, chromogenic substrate, cleansing solution, Sample dilution and stop buffer, it is characterized in that: described reaction film bar is made up of carrier and the nitrocellulose filter be fixed on carrier or nylon membrane, described nitrocellulose filter or nylon membrane contain: respectively by dsDNA, Sm/RNP, CCP, SSA, SSB, GAD, ICA, IA-2A, TG, natural or the recombinant antigen bag of in totally 10 kinds at least two kinds of AMA-M2 by more than two parallel detection lines, article 1, bag is the high concentration quality control band of 30-50 μ g/ml by concentration, article 1, bag is concentration quality control band in 15-25 μ g/ml by concentration, and 1 bag is the low concentration quality control band of 1-10 μ g/ml by concentration.
Described carrier is the PVC board of biadhesive, and front and described nitrocellulose membrane or nylon membrane fixed bonding, back side fixed bonding has transparent PET bottom lining plate.
Described high concentration quality control band, intermediate concentration quality control band and low concentration quality control band are by human IgG, or against murine IgG or anti-sheep IgG line forms.
Described enzyme conjugates is the mouse-anti human IgG of horseradish peroxidase mark; Described chromogenic substrate is tetramethyl benzidine; Described cleansing solution is 20 × Tris damping fluid; Described Sample dilution is the Tris damping fluid containing sealer; Described stop buffer is 0.1M/L sulfuric acid.
Prepare the method for described kit, include reaction film bar preparation method, it is characterized in that described reaction film bar preparation method includes following steps:
1) prepare spotting solution: by dsDNA, Sm/RNP, CCP, SSA, SSB, GAD, ICA, IA-2A, TG and AMA-M2 antigen, and human IgG, against murine IgG or anti-sheep IgG are mixed with and wrap by concentration accordingly;
2) immobilized antigen: according to the size of Western blotting sample applicator, nitrocellulose membrane or nylon membrane are cut into diaphragm, by physisorption and covalently bound mode, by 1) in the spotting solution that configures be fixed on diaphragm with certain ordering, form independently detection line;
3) close: be placed on by diaphragm in the solution of unrelated protein or serum and close its non-point sample position, described unrelated protein or serum comprise skimmed milk power, casein, bovine serum albumin(BSA), saturated lysine and calf serum;
4) dry: the film strip closed to be put into 37 DEG C of constant temperature ovens dry 2 hours;
5) pad pasting: the front dried diaphragm being affixed on the PVC board of biadhesive, the back side of PVC board is stand-by;
6) cut film: by 5) in the diaphragm that posts be cut into the band of one fixed width according to independent detection line;
7) assemble: by mould, by 6) in band be affixed on transparent PET bottom lining plate in order;
8) slitting: the band assembled is cut into single part reaction film bar with cutting cutter.
2) after step completes point sample, diaphragm is taken out ambient temperatare from Western blotting sample applicator and puts 1 hour.
The point sample amount of immobilized antigen and Quality Control controls at 10ng ~ 30ng.
The width of quality control band is 7mm, and the width of antigen bands is 5mm.
Relative to prior art, tool of the present invention has the following advantages:
The present invention is by a clinical sample and the multiple autoantibody of one-time detection reaction detection, overcome that single autoantibody detection sensitivity and specificity are not enough, that several conditions related auto-antibodies detects separately operationally one by one is loaded down with trivial details, greatly improve the accuracy that detection efficiency and result judge.The autoimmune disease related to comprises systemic loupus erythematosus, rheumatoid arthritis, Sjogren syndrome, type i diabetes, autoimmune thyroid disease, primary biliary cirrhosis of liver.Also have in addition:
1, antigen reaction film bar wrapping quilt is the native antigen extracted from animal tissue or the recombinant protein adopting the mode of insect cell or baculoviral eukaryotic expression to obtain, and improves the Sensitivity and Specificity of diagnosing autoimmune diseases;
2, the full-automatic point sample instrument of antigen coated employing, ensure the accuracy of quality control band and each antigen zone position on film bar, and rational sorting can be carried out according to detection demand, be different from traditional SDS-PAGE technology by molecular size range, antigen to be separately transferred on blotting membrane successively, ensure that the integrality of antigenic determinant conformation simultaneously;
3, single part film bar is coated with 10 kinds of antigens simultaneously, can realize a clinical sample and once test and can detect 10 kinds of antibody at most simultaneously, the clinical diagnosis for various autoimmune disease provides serology foundation;
4, have high concentration quality control band, middle concentration quality control band and low concentration quality control band, result is judged more directly perceived accurate, the course of disease for autoimmune disease is monitored and treatment prognosis provides extensive guide.
Accompanying drawing explanation
Fig. 1 is antigen and quality control band distribution schematic diagram on reaction film bar in kit of the present invention;
Fig. 2 is antigen and quality control band schematic cross-section on reaction film bar in kit of the present invention.
Embodiment
The invention discloses and a kind ofly detect HP immunoblotting kit of various autoimmune disease relevant antibodies and preparation method thereof, those skilled in the art can use for reference present disclosure, are realized by suitable improving technique parameter.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the art, they are all deemed to be included within the present invention's technical scheme required for protection.Product of the present invention and application are described by preferred embodiment, related personnel obviously can not depart from content of the present invention, spirit and scope methods and applications as herein described are changed or suitably change with combination, realize and apply the technology of the present invention.
For making the present invention easier to understand, below in conjunction with specific embodiment, set forth the present invention further, these embodiments are only not used in for illustration of the present invention and limit the scope of the invention.
The HP immunoblotting kit of detection autoimmune disease antibody of the present invention comprises: reaction film bar, enzyme conjugates, chromogenic substrate, cleansing solution, Sample dilution and stop buffer; Described reaction film bar is made up of carrier and the nitrocellulose filter be fixed on carrier or nylon membrane, described nitrocellulose filter or nylon membrane contain: respectively by dsDNA, Sm/RNP, CCP, SSA, SSB, GAD, ICA, IA-2A, TG, AMA-M2 totally 10 kinds of natural or recombinant antigen bags by 10 parallel detection lines 1 ~ 10, article 1, bag is the high concentration quality control band 11 of 30-50 μ g/ml by concentration, article 1, bag is concentration quality control band 12 in 15-25 μ g/ml by concentration, and 1 bag is the low concentration quality control band 13 of 1-10 μ g/ml by concentration.Realize single part film bar is coated with 10 kinds of antigens simultaneously, a clinical sample and the multiple autoantibody of one-time detection reaction detection can be realized, 10 kinds of antibody can be detected at most simultaneously, clinical diagnosis for various autoimmune disease provides serology foundation, as: autoimmune disease comprises systemic loupus erythematosus, rheumatoid arthritis, Sjogren syndrome, type i diabetes, autoimmune thyroid disease, primary biliary cirrhosis of liver.
Shown in seeing figures.1.and.2, the carrier of described reaction film bar is the PVC board 15 of biadhesive, and front and the described nitrocellulose membrane formed after detection line and quality control band or nylon membrane 16 fixed bonding, back side fixed bonding has transparent PET bottom lining plate 14.Described high concentration quality control band, intermediate concentration quality control band and low concentration quality control band are by human IgG, or against murine IgG or anti-sheep IgG line forms.Described enzyme conjugates is the mouse-anti human IgG of horseradish peroxidase mark; Described chromogenic substrate is tetramethyl benzidine; Described cleansing solution is 20 × Tris damping fluid; Described Sample dilution is the Tris damping fluid containing sealer; Described stop buffer is 0.1M/L sulfuric acid.
The preparation method of the HP immunoblotting kit of detection autoimmune disease antibody of the present invention, includes reaction film bar preparation method, it is characterized in that described reaction film bar preparation method includes following steps:
1) prepare spotting solution: by dsDNA, Sm/RNP, CCP, SSA, SSB, GAD, ICA, IA-2A, TG and AMA-M2 antigen, and human IgG, against murine IgG or anti-sheep IgG Quality Control are mixed with and wrap by concentration accordingly;
2) immobilized antigen: according to the size of Western blotting sample applicator, nitrocellulose membrane or nylon membrane are cut into diaphragm, by physisorption and covalently bound mode, by 1) in the spotting solution that configures be fixed on diaphragm with certain ordering, form independently detection line; The point sample amount of immobilized antigen and Quality Control controls at 10ng ~ 30ng; The width of quality control band is 7mm, and the width of antigen bands is 5mm; After completing point sample, diaphragm is taken out ambient temperatare from Western blotting sample applicator and puts 1 hour.
3) close: be placed on by diaphragm in the solution of unrelated protein or serum and close its non-point sample position, described unrelated protein or serum comprise skimmed milk power, casein, bovine serum albumin(BSA), saturated lysine and calf serum; The preferably skimmed milk power of 5%, at room temperature closes 0.5 hour.With the concentrated cleaning solution washing through 20 times of dilutions.
4) dry: the film strip closed to be put into 37 DEG C of constant temperature ovens dry 2 hours;
5) pad pasting: the front dried diaphragm being affixed on the PVC board of biadhesive, the back side of PVC board is stand-by;
6) cut film: by 5) in the diaphragm that posts be cut into the band of one fixed width according to independent detection line; Wherein, the width of 3 quality control bands is 7mm, and the width of 10 kinds of antigen bands is 5mm.
7) assemble: by mould, by 6) in band be affixed on transparent PET bottom lining plate in order;
8) slitting: with cutting cutter, the band assembled is cut into single part reaction film bar, preferable width is 2mm.
The using method of kit of the present invention
Sample requirement: the blood plasma of human serum or EDTA, heparin or citrate anticoagulation.
1, cleansing solution preparation: with 19 parts of distilled water dilutings 1 part 20 × concentrated cleaning solution, mix, for subsequent use.
2, detecting step
1) reaction film bar is put into hatch dish (General experimental consumptive material), add the Sample dilution wetting film bar of 1ml, move after 1 minute and abandon dilution;
2) add the sample that 1ml has diluted, put shaking table jog, incubated at room 30 minutes, then the sample that diluted of reject, does not stay raffinate;
3) with 1.5ml cleansing solution washing reaction film bar, put shaking table jog, each 5 minutes, washed rear reject cleansing solution, repeat 3 times;
4) add 1ml enzyme conjugates solution, put shaking table jog, incubated at room 30 minutes, reject enzyme conjugates solution;
5) step 3) is repeated;
6) add 1ml Chromogenic Substrate Solution, put shaking table jog, room temperature lucifuge hatches 10 minutes, reject substrate solution;
7) with the washing of 1.5ml distilled water, shaking table jog is put, reject distilled water after 1 minute;
8) add 1ml stop buffer, put shaking table jog, incubated at room 5 minutes, reject stop buffer;
9) take out reaction film bar, blot the residual liquid on it with thieving paper;
3, result judges
After having reacted, range estimation, hand paste can be adopted to the fixing card of scanner scanning then by specific interpretation software or use full automatic interpretation analytical instrument to carry out interpretation to testing result, the numerical value that result can be judged as feminine gender, the weak positive, moderate positive, strong positive or export as quantizing.
Claims (8)
1. detect the HP immunoblotting kit of autoimmune disease antibody, comprise: reaction film bar, enzyme conjugates, chromogenic substrate, cleansing solution, Sample dilution and stop buffer, it is characterized in that: described reaction film bar is made up of carrier and the nitrocellulose filter be fixed on carrier or nylon membrane, described nitrocellulose filter or nylon membrane contain: respectively by dsDNA, Sm/RNP, CCP, SSA, SSB, GAD, ICA, IA-2A, TG, AMA-M2 totally 10 kinds of natural or recombinant antigen bags by 10 parallel detection lines, article 1, bag is the high concentration quality control band of 30-50 μ g/ml by concentration, article 1, bag is concentration quality control band in 15-25 μ g/ml by concentration, and 1 bag is the low concentration quality control band of 1-10 μ g/ml by concentration, described high concentration quality control band, intermediate concentration quality control band and low concentration quality control band are by human IgG, or against murine IgG or anti-sheep IgG line forms.
2. the kit according to patent requirements 1, is characterized in that: described carrier is the PVC board of biadhesive, and front and described nitrocellulose membrane or nylon membrane fixed bonding, back side fixed bonding has transparent PET bottom lining plate.
3. the kit according to patent requirements 1, is characterized in that: described enzyme conjugates is the mouse-anti human IgG of horseradish peroxidase mark; Described chromogenic substrate is tetramethyl benzidine; Described cleansing solution is 20 × Tris damping fluid; Described Sample dilution is the Tris damping fluid containing sealer; Described stop buffer is 0.1mol/L sulfuric acid.
4. the preparation method of kit described in any one of claim 1 ~ 3, includes reaction film bar preparation method, it is characterized in that described reaction film bar preparation method includes following steps:
1) prepare spotting solution: by dsDNA, Sm/RNP, CCP, SSA, SSB, GAD, ICA, IA-2A, TG and AMA-M2 antigen, and human IgG, against murine IgG or anti-sheep IgG Quality Control are mixed with and wrap by concentration accordingly;
2) immobilized antigen: according to the size of Western blotting sample applicator, nitrocellulose membrane or nylon membrane are cut into diaphragm, by physisorption and covalently bound mode, by 1) in the spotting solution that configures be fixed on diaphragm with certain ordering, form independently detection line and quality control band;
3) close: be placed on by diaphragm in the solution of unrelated protein or serum and close its non-point sample position, described unrelated protein or serum comprise skimmed milk power, casein, bovine serum albumin(BSA), saturated lysine or calf serum;
4) dry: the film strip closed to be put into 37 DEG C of constant temperature ovens dry 2 hours;
5) pad pasting: the front dried diaphragm being affixed on the PVC board of biadhesive, the back side of PVC board is stand-by;
6) cut film: by 5) in the diaphragm that posts be cut into the band of one fixed width according to independent detection line;
7) assemble: by mould, by 6) in band be affixed on transparent PET bottom lining plate in order;
8) slitting: the band assembled is cut into single part reaction film bar with cutting cutter.
5. preparation method according to claim 4, is characterized in that: described closed unrelated protein selects the skimmed milk power of 5%, at room temperature closes 0.5 hour.
6. preparation method according to claim 4, is characterized in that: 2) after step completes point sample, diaphragm is taken out ambient temperatare from Western blotting sample applicator and puts 1 hour.
7. preparation method according to claim 4, is characterized in that: the point sample amount of immobilized antigen and Quality Control controls at 10ng ~ 30ng.
8. preparation method according to claim 4, is characterized in that: the width of quality control band is 7mm, and the width of detection line is 5mm.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067119A (en) * | 1992-06-25 | 1992-12-16 | 江苏省中医院 | The solidified mouse thyroglobulin detects antithyroglobulin antibodies in the human blood |
| CN1338633A (en) * | 2001-09-29 | 2002-03-06 | 上海晶泰生物技术有限公司 | Protein chip for diagnosing autoimmune diseases |
| CN1566955A (en) * | 2003-06-20 | 2005-01-19 | 黄勤 | Thyrotropic hormone diagnosis reagent paper and use thereof |
| CN1719256A (en) * | 2005-07-15 | 2006-01-11 | 北京倍爱康生物技术股份有限公司 | Magnetic separation enzymatic chemical luminous immune detection method of human thyroglobulin antibody |
| CN1958808A (en) * | 2006-08-11 | 2007-05-09 | 北京爱普益生物科技有限公司 | Gene chip, detection method, and kit for detecting virus infected by oxen |
| CN101226192A (en) * | 2008-02-03 | 2008-07-23 | 山东省医药生物技术研究中心 | Colour development protein chip using BCIP/NBT as substrate as well as application in detection of autoantibody thereof |
| CN101329341A (en) * | 2008-07-09 | 2008-12-24 | 黑龙江美康汇融生物技术股份有限公司 | Reagent kit for detecting autoimmunity disease related antinuclear antibodies spectrum and preparation method thereof |
| CN101377511A (en) * | 2008-04-16 | 2009-03-04 | 北京科美东雅生物技术有限公司 | Chemiluminescence immune analysis determination reagent kit for detecting thyroglobulin antibody |
| CN102323404A (en) * | 2011-05-18 | 2012-01-18 | 高翔 | Autoimmunity antibody detection kit and detection method |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040048274A1 (en) * | 2000-10-17 | 2004-03-11 | Morten Breindahl | Assay for directly detecting an inflammatory indicator in a body fluid sample |
| CN100504386C (en) * | 2003-07-31 | 2009-06-24 | 上海数康生物科技有限公司 | Miniature reaction cup type protein chip |
| US20070190044A1 (en) * | 2003-08-15 | 2007-08-16 | Barabas Arpad Z | Compositions and methods for manipulating levels of antigen-specific antibodies in a mammal |
| US20070148704A1 (en) * | 2005-10-06 | 2007-06-28 | Ursula Klause | Anti-CCPand antinuclear antibodies in diagnosis of rheumatoid arthritis |
| CN1866013B (en) * | 2006-05-31 | 2010-08-04 | 山东省医药生物技术研究中心 | Liquid phase chip for parallel detection of autoantibodies, preparation method and application thereof |
-
2013
- 2013-01-23 CN CN201310024747.6A patent/CN103105489B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067119A (en) * | 1992-06-25 | 1992-12-16 | 江苏省中医院 | The solidified mouse thyroglobulin detects antithyroglobulin antibodies in the human blood |
| CN1338633A (en) * | 2001-09-29 | 2002-03-06 | 上海晶泰生物技术有限公司 | Protein chip for diagnosing autoimmune diseases |
| CN1566955A (en) * | 2003-06-20 | 2005-01-19 | 黄勤 | Thyrotropic hormone diagnosis reagent paper and use thereof |
| CN1719256A (en) * | 2005-07-15 | 2006-01-11 | 北京倍爱康生物技术股份有限公司 | Magnetic separation enzymatic chemical luminous immune detection method of human thyroglobulin antibody |
| CN1958808A (en) * | 2006-08-11 | 2007-05-09 | 北京爱普益生物科技有限公司 | Gene chip, detection method, and kit for detecting virus infected by oxen |
| CN101226192A (en) * | 2008-02-03 | 2008-07-23 | 山东省医药生物技术研究中心 | Colour development protein chip using BCIP/NBT as substrate as well as application in detection of autoantibody thereof |
| CN101377511A (en) * | 2008-04-16 | 2009-03-04 | 北京科美东雅生物技术有限公司 | Chemiluminescence immune analysis determination reagent kit for detecting thyroglobulin antibody |
| CN101329341A (en) * | 2008-07-09 | 2008-12-24 | 黑龙江美康汇融生物技术股份有限公司 | Reagent kit for detecting autoimmunity disease related antinuclear antibodies spectrum and preparation method thereof |
| CN102323404A (en) * | 2011-05-18 | 2012-01-18 | 高翔 | Autoimmunity antibody detection kit and detection method |
Non-Patent Citations (6)
| Title |
|---|
| Eli Olaussen,et al..Screening Tests for Antinuclear Antibodies (ANA):Selective Use of Central Nuclear Antigens as a Rational Basis for Screening by ELISA.《Journal of Autoimmunity》.1999,第13卷(第1期),第95-102页. * |
| 张如根 等.ICA512/IA-2抗体及GAD抗体联合检测筛查自身免疫中介1型糖尿病.《上海医学》.2001,第24卷(第4期),第214-216页. * |
| 抗CCP抗体斑点免疫印迹实验测定方法的建立与初步应用;虞伟 等;《中国实验诊断学》;20080630;第12卷(第6期);摘要 * |
| 自身免疫与慢性荨麻疹;孙仁山 等;《国外医学皮肤性病学分册》;20001231;第26卷(第3期);第135-138页 * |
| 自身免疫肝病自身抗体检测的临床意义;李全焕 等;《标记免疫分析与临床》;20120430;第19卷(第2期);第114-115页 * |
| 血清GADA、ICA、IAA、IA-2A的测定在糖尿病分型中的应用及诊断价值;茅华英 等;《浙江中医药大学学报》;20090630;第33卷(第4期);第536页"2 研究指标和方法"部分 * |
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