CN114264806B - Membrane strip for immunoblotting and manufacturing method thereof - Google Patents
Membrane strip for immunoblotting and manufacturing method thereof Download PDFInfo
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- CN114264806B CN114264806B CN202111541352.4A CN202111541352A CN114264806B CN 114264806 B CN114264806 B CN 114264806B CN 202111541352 A CN202111541352 A CN 202111541352A CN 114264806 B CN114264806 B CN 114264806B
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- film
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- cutting
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- 239000012528 membrane Substances 0.000 title claims abstract description 34
- 238000003119 immunoblot Methods 0.000 title claims abstract description 25
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 18
- 239000000758 substrate Substances 0.000 claims abstract description 40
- 238000002360 preparation method Methods 0.000 claims abstract description 6
- 238000005520 cutting process Methods 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 9
- 230000000149 penetrating effect Effects 0.000 claims description 7
- 239000000427 antigen Substances 0.000 claims description 6
- 102000036639 antigens Human genes 0.000 claims description 6
- 108091007433 antigens Proteins 0.000 claims description 6
- 239000003292 glue Substances 0.000 claims description 6
- 239000000853 adhesive Substances 0.000 claims description 3
- 230000001070 adhesive effect Effects 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 3
- 238000003908 quality control method Methods 0.000 claims description 3
- 238000005096 rolling process Methods 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 8
- 238000005259 measurement Methods 0.000 abstract description 6
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 3
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 239000002390 adhesive tape Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000010981 drying operation Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a membrane strip for immunoblotting and a manufacturing method thereof, wherein a positioning tool is used for fixing a substrate, a limiting groove on the positioning tool is used for limiting each strip NC membrane to be attached to the substrate, additional measurement is not needed, attachment precision is ensured, antibody lines on the prepared membrane strip are mutually parallel, detection precision of the membrane strip is ensured, meanwhile, the preparation process is simple, and the operation strength of operators is greatly reduced.
Description
Technical Field
The invention relates to the technical field of NC film production, in particular to a film strip for immunoblotting and a manufacturing method thereof.
Background
Immunoblotting (western blotting) is a hybridization technique that combines high-resolution gel electrophoresis with immunochemical analysis techniques. The immunoblotting method has the advantages of large analysis capacity, high sensitivity, strong specificity and the like, and is a most commonly used method for detecting protein characteristics, expression and distribution, such as qualitative and quantitative detection of tissue antigens, mass measurement of polypeptide molecules, detection of antibodies or antigens of viruses and the like. It is to transfer the protein to a membrane strip and then detect it with an antibody. For known expressed proteins, the corresponding antibodies can be used as primary antibodies, and the expression products of the novel genes can be detected by antibodies to the fusion moiety.
The membrane strip is a critical detection basis in an immunoblotting method, is a nitrocellulose membrane (nitrocellulose filter membrane, NC membrane for short), is also an immune reaction place, and is one of the most important consumables in biological tests.
In the existing film strip, a plurality of NC films marked with various antibody lines are sequentially attached to a substrate in the preparation process, and then cut and bagged. At present, an operator sticks NC films marked with various antibody lines on a substrate by means of sticking experience and manual measurement, and errors exist in manual measurement, so that the distance between the antibody lines and the absolute parallelism between the antibody lines cannot be ensured, the antibody lines deviate, and the detection precision is affected.
In view of the above-mentioned drawbacks, the present inventors have actively studied and innovated to create a membrane strip for immunoblotting with high processing accuracy and a method for producing the same, which are more industrially useful.
Disclosure of Invention
The invention aims to provide a membrane strip for immunoblotting and a manufacturing method thereof, which can greatly improve the detection precision of the membrane strip.
To achieve the purpose, the invention adopts the following technical scheme:
a method for manufacturing a membrane strip for immunoblotting comprises the following steps:
s1, cutting an NC film according to set width and length requirements;
s2, cutting back glue with corresponding width and length according to the width and length of the NC film in the step S1, and attaching the back glue to one surface of the NC film in the step S1;
s3, rolling and cutting the NC film with the back adhesive into a plurality of strips with set widths;
s4, marking antibody lines on the NC films of the strips according to the requirements, and marking the marked NC films;
s5, drying each long NC film in the step S4;
s6, providing a positioning tool, wherein the positioning tool comprises a supporting bottom plate, a plurality of parallel ribs are sequentially arranged on the supporting bottom plate at intervals along the length direction of the supporting bottom plate, limiting grooves are formed between adjacent ribs, and the width of each limiting groove is matched with the width of the NC film in the step S5; the supporting bottom plate is provided with a slot which penetrates through the rib from one side to the other side of the supporting bottom plate; the support bottom plate is connected with at least two baffles at one end of the slot, and the support bottom plate is connected with two clamps at one end of the support bottom plate, which is provided with the baffles;
s7, cutting a substrate according to the set width and length requirements, penetrating the substrate into the slot and propping against the baffle, attaching a label to the substrate between the baffle and the adjacent ribs, pressing the substrate on the supporting bottom plate by using the clamp, and sequentially placing NC films of the strips in the step S5 into the limiting grooves according to the planned quality control, the type, the sequence of antigens and attaching the NC films to the substrate;
s8, cutting off NC films of the parts with the more substrates after the NC films are attached, opening a clamp, and extracting the substrates with the NC films attached;
and S9, cutting the substrate with the NC film stuck in the step S8 into film strips with required width by using a film cutting machine.
As a preferable scheme of the method for manufacturing the membrane strip for immunoblotting, a plurality of scale marks are further arranged between the adjacent ribs of the supporting bottom plate and used for positioning when the antibody lines are marked.
As a preferable mode of the method for producing a film strip for immunoblotting described above, in the step S3, the NC film is slit into a plurality of strips having a predetermined width by a slitting mill.
As a preferable mode of the method for producing a membrane strip for immunoblotting described above, in the step S4, an antibody line is drawn on the NC membrane by a film drawing machine.
In the step S5, the drying temperature is 35-37 ℃ and the drying time is 2-3 hours.
As a preferable mode of the method for producing a membrane strip for immunoblotting, the substrate in the step S7 is a PVC plate or a PET plate.
The invention also provides an immunoblotting membrane strip which is manufactured by adopting the manufacturing method of the immunoblotting membrane strip, and comprises a strip-shaped substrate and a plurality of NC membranes attached to the substrate at intervals, wherein at least one antibody line is marked on the NC membrane, and the antibody lines are mutually parallel.
The beneficial effects of the invention are as follows: the substrate is fixed by utilizing the positioning tool, each strip NC film to be attached to the substrate is limited by utilizing the limiting groove on the positioning tool, additional measurement is not needed, the attaching precision is ensured, all antibody lines on the prepared film strips are mutually parallel, the detecting precision of the film strips is ensured, meanwhile, the preparation flow is simple, and the operation intensity of operators is greatly reduced.
Drawings
FIG. 1 is a flow chart of the production of a membrane strip for immunoblotting of the present invention;
fig. 2 is a schematic structural diagram of a positioning tool related to a manufacturing method of the membrane strip for immunoblotting of the present invention;
FIG. 3 is a front view of the fixture in position;
FIG. 4 is a film strip made in accordance with the present invention.
In the figure: 10-supporting bottom plates, 11-ribs, 12-limit grooves, 13-slots, 14-baffle plates, 15-clamps, 16-scale line grooves, 20-substrates, 30-antibody lines and 40-NC films.
Detailed Description
In order to make the technical problems solved by the present invention, the technical solutions adopted and the technical effects achieved more clear, the technical solutions of the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to fall within the scope of the invention.
In the description of the present invention, unless explicitly stated and limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
In the present invention, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.
As shown in fig. 1 to 3, the method for producing the immunoblotting membrane strip of the present invention comprises the steps of:
s1, cutting the NC film according to the set width and length requirements.
S2, cutting the back glue with the corresponding width and length according to the width and length of the NC film in the step S1, and attaching the back glue to one surface of the NC film in the step S1. The double-sided adhesive tape can be used for pasting the back of the NC film, air bubbles cannot be formed in a flattening manner, and the NC film is protected by the protection paper before pasting the double-sided adhesive tape, so that the NC film is prevented from being polluted.
S3, rolling and cutting the NC film with the back adhesive into a plurality of strips with set widths. The NC film may be slit into a plurality of strips of a set width using a slitting mill.
And S4, scribing antibody lines on the NC films of the strips according to requirements, and marking the scribed NC films. The antibody lines are marked on the NC films by using a film marking instrument, and the number of marked antigens is different from one NC film to another because of a plurality of indexes to be detected and a plurality of combinations to be selected.
And S5, drying each long NC film in the step S4. The drying temperature is 35-37 ℃, the drying time is 2-3 hours, and the drying operation can effectively avoid the diffusion and the widening of the antibody line, so that the detection fluorescence of the antibody line is concentrated.
S6, providing a positioning tool, wherein the positioning tool comprises a supporting bottom plate 10, wherein a plurality of parallel ribs 11 are sequentially arranged on the supporting bottom plate 10 at intervals along the length direction of the supporting bottom plate, limiting grooves 12 are formed between every two adjacent ribs 11, and the width of each limiting groove 12 is matched with the width of the NC film in the step S5; the supporting bottom plate 10 is provided with a slot 13 penetrating from one side to the other side and penetrating each rib 11; the support base plate 10 is connected with at least two baffles 14 at one end of the slot 13, and the support base plate 10 is connected with two clamps 15 at its end provided with baffles 14.
S7, cutting the substrate according to the set width and length requirements, penetrating the substrate into the slot 13 and abutting against the baffle plate 14, labeling the substrate between the baffle plate 14 and the adjacent rib 11, pressing the substrate on the supporting bottom plate 10 by using the clamp 15, and sequentially placing the NC films of the strips in the step S5 into the limiting grooves 12 according to the planned quality control, the type, the model and the sequence of antigens and attaching the NC films to the substrate. The substrate in the invention is a PVC board or a PET board plastic board. The substrate is fixed by utilizing the positioning tool, each strip NC film to be attached to the substrate is limited by utilizing the limiting groove 12 on the positioning tool, additional measurement is not needed, the attaching precision is ensured, the antibody lines on the prepared film strips are mutually parallel, the detecting precision of the film strips is ensured, meanwhile, the preparation flow is simple, and the operation intensity of operators is greatly reduced.
S8, cutting off NC films of the parts with the more substrates after the NC films are attached, and then opening a clamp to withdraw the substrates with the NC films attached.
S9, cutting the substrate with the NC film stuck in the step S8 into film strips with required width by using a film cutting machine, and finally placing the film strips into special aluminum film bags.
As a preferred embodiment of the present invention, the present invention further provides a plurality of graduation marks 16 between the adjacent ribs 11 of the support base 10. The scale marks 16 can facilitate scribing and positioning of the NC film again according to the size requirement after the NC film is placed in the limiting groove 12, and the scribing precision is ensured.
As shown in fig. 4, the immunoblotting membrane strip of the present invention is manufactured by the method for manufacturing the immunoblotting membrane strip, and comprises a strip-shaped substrate 20 and a plurality of NC films 40 attached to the substrate 20 at intervals, wherein at least one antibody line 30 is marked on the NC film 40, and the antibody lines 30 are parallel to each other. The antibody lines 30 on the membrane strip prepared by the preparation method are mutually parallel, so that the detection precision of the membrane strip is ensured.
The technical principle of the present invention is described above in connection with the specific embodiments. The description is made for the purpose of illustrating the general principles of the invention and should not be taken in any way as limiting the scope of the invention. Other embodiments of the invention will be apparent to those skilled in the art from consideration of this specification without undue burden.
Claims (6)
1. The preparation method of the membrane strip for immunoblotting is characterized by comprising the following steps:
s1, cutting an NC film according to set width and length requirements;
s2, cutting back glue with corresponding width and length according to the width and length of the NC film in the step S1, and attaching the back glue to one surface of the NC film in the step S1;
s3, rolling and cutting the NC film with the back adhesive into a plurality of strips with set widths;
s4, marking antibody lines on the NC films of the strips according to the requirements, and marking the marked NC films;
s5, drying each long NC film in the step S4;
s6, providing a positioning tool, wherein the positioning tool comprises a supporting bottom plate (10), a plurality of parallel ribs (11) are sequentially arranged on the supporting bottom plate (10) at intervals along the length direction of the supporting bottom plate, limiting grooves (12) are formed between every two adjacent ribs (11), and the width of each limiting groove (12) is matched with that of the NC film in the step S5; the supporting bottom plate (10) is provided with a slot (13) penetrating from one side to the other side and penetrating through each rib (11); at least two baffles (14) are connected to the support bottom plate (10) at one end of the slot (13), and two clamps (15) are connected to the support bottom plate (10) at one end of the support bottom plate where the baffles (14) are arranged;
s7, cutting a substrate according to the set width and length requirements, penetrating the substrate into the slot (13) and propping against the baffle (14), attaching a label to the substrate between the baffle (14) and the adjacent rib (11), pressing the substrate on the supporting bottom plate (10) by using the clamp (15), and sequentially placing the NC films of the strips in the step S5 into the limiting grooves (12) according to the planned quality control and the type, model and sequence of antigens and attaching the NC films to the substrate;
s8, cutting off NC films of the parts with the more substrates after the NC films are attached, and then opening a clamp (15) to withdraw the substrates with the NC films attached;
and S9, cutting the substrate with the NC film stuck in the step S8 into film strips with required width by using a film cutting machine.
2. The method for producing the immunoblotting membrane strip according to claim 1, wherein a plurality of graduation marks (16) are further provided between the adjacent ribs (11) on the support base plate (10).
3. The method of producing a film strip for immunoblotting according to claim 1, wherein in the step S3, the NC film is slit into a plurality of strips having a predetermined width by a slitting machine.
4. The method of producing a membrane strip for immunoblotting according to claim 1, wherein in step S4, an antibody line is drawn on the NC membrane by a film drawing machine.
5. The method according to claim 1, wherein in the step S5, the drying temperature is 35-37 ℃ and the drying time is 2-3 hours.
6. The method of claim 1, wherein the substrate in the step S7 is a PVC plate or a PET plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111541352.4A CN114264806B (en) | 2021-12-16 | 2021-12-16 | Membrane strip for immunoblotting and manufacturing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111541352.4A CN114264806B (en) | 2021-12-16 | 2021-12-16 | Membrane strip for immunoblotting and manufacturing method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114264806A CN114264806A (en) | 2022-04-01 |
| CN114264806B true CN114264806B (en) | 2024-03-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111541352.4A Active CN114264806B (en) | 2021-12-16 | 2021-12-16 | Membrane strip for immunoblotting and manufacturing method thereof |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003062824A1 (en) * | 2002-01-23 | 2003-07-31 | Boditech Inc. | Lateral flow quantitative assay method and strip and laser-induced fluoerescence detection device therefor |
| CN101266246A (en) * | 2008-04-30 | 2008-09-17 | 天津中新科炬生物制药有限公司 | HIV antibody and antigen combined rapid detection reagent kit |
| CN102621302A (en) * | 2012-03-22 | 2012-08-01 | 四川省新成生物科技有限责任公司 | Linear immune detection film bar and manufacturing process thereof |
| CN103105489A (en) * | 2013-01-23 | 2013-05-15 | 深圳市亚辉龙生物科技有限公司 | Western blot kit for detecting antibody of autoimmune disease and preparation method thereof |
| CN105823873A (en) * | 2015-07-03 | 2016-08-03 | 深圳市亚辉龙生物科技股份有限公司 | Immunoblotting kit for detecting autoimmune nephrosis and preparation method thereof |
| CN207764234U (en) * | 2018-02-20 | 2018-08-24 | 珠海维塔贝拉生物技术有限公司 | Immunochromatography plastic bottom board pastes nitrocellulose film device |
| CN209215404U (en) * | 2018-11-29 | 2019-08-06 | 莫纳(连云港)生物科技有限公司 | A kind of blotting membrane clamping and positioning device |
| CN112461825A (en) * | 2020-11-10 | 2021-03-09 | 苏州慧源安食检测科技有限公司 | Rapid detection method for rapidly and quantitatively detecting carbendazim pesticide |
| CN114534997A (en) * | 2022-02-25 | 2022-05-27 | 河南先研生物科技有限公司 | Point membrane device for preparing detection card |
-
2021
- 2021-12-16 CN CN202111541352.4A patent/CN114264806B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003062824A1 (en) * | 2002-01-23 | 2003-07-31 | Boditech Inc. | Lateral flow quantitative assay method and strip and laser-induced fluoerescence detection device therefor |
| CN101266246A (en) * | 2008-04-30 | 2008-09-17 | 天津中新科炬生物制药有限公司 | HIV antibody and antigen combined rapid detection reagent kit |
| CN102621302A (en) * | 2012-03-22 | 2012-08-01 | 四川省新成生物科技有限责任公司 | Linear immune detection film bar and manufacturing process thereof |
| CN103105489A (en) * | 2013-01-23 | 2013-05-15 | 深圳市亚辉龙生物科技有限公司 | Western blot kit for detecting antibody of autoimmune disease and preparation method thereof |
| CN105823873A (en) * | 2015-07-03 | 2016-08-03 | 深圳市亚辉龙生物科技股份有限公司 | Immunoblotting kit for detecting autoimmune nephrosis and preparation method thereof |
| CN207764234U (en) * | 2018-02-20 | 2018-08-24 | 珠海维塔贝拉生物技术有限公司 | Immunochromatography plastic bottom board pastes nitrocellulose film device |
| CN209215404U (en) * | 2018-11-29 | 2019-08-06 | 莫纳(连云港)生物科技有限公司 | A kind of blotting membrane clamping and positioning device |
| CN112461825A (en) * | 2020-11-10 | 2021-03-09 | 苏州慧源安食检测科技有限公司 | Rapid detection method for rapidly and quantitatively detecting carbendazim pesticide |
| CN114534997A (en) * | 2022-02-25 | 2022-05-27 | 河南先研生物科技有限公司 | Point membrane device for preparing detection card |
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| CN114264806A (en) | 2022-04-01 |
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