CN209215404U - A kind of blotting membrane clamping and positioning device - Google Patents
A kind of blotting membrane clamping and positioning device Download PDFInfo
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- CN209215404U CN209215404U CN201821992313.XU CN201821992313U CN209215404U CN 209215404 U CN209215404 U CN 209215404U CN 201821992313 U CN201821992313 U CN 201821992313U CN 209215404 U CN209215404 U CN 209215404U
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- blotting membrane
- attaching plate
- grip block
- lower attaching
- positioning column
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Abstract
The utility model provides a kind of blotting membrane clamping and positioning device, including auxiliary backplane and clamp assemblies, blotting membrane is adsorbed on lower attaching plate surface by the adsorption capacity monolithic stability of moisture film, blotting membrane upper end is clamped between lower attaching plate and upper tabletting, the part that blotting membrane enters test solution can be detached from lower attaching plate and sufficiently be reacted with test solution, and blotting membrane is detached from after test solution and meeting monolithic stability is adsorbed on lower attaching plate surface；The third positioning column of auxiliary backplane and the same straight line of the first positioning column of main grip block and the second positioning column are arranged, the both ends of lower attaching plate are positioned respectively, improve the verticality precision of the relatively main grip block of lower attaching plate, ensure that blotting membrane high position accuracy between each test solution slot of immunoblotting assay equipment is mobile, the arrangement of test solution slot can be more compact, cavity opening be can be made smaller, and save the single liquid volume added of antibody test solution, immunoblotting assay equipment is also made to more small and exquisite.
Description
Technical field
The utility model relates to a kind of experiment auxiliary appliance, especially a kind of blotting membrane clamping and positioning device.
Background technique
Western blot test is also known as Western Blot Assay (Western Blot).It is biochemistry and immunogenetics
In a kind of common experimental method, be that the cell or tissue gross protein after electrophoretic separation is transferred to solid phase carrier from gel
On blotting membrane, then using on blotting membrane protein or polypeptide as antigen, play immune response with corresponding antibody, then with enzyme or same
The secondary antibody of position element label reacts, and the specific mesh of electrophoretic separation is finally detected by substrate colour developing or autoradiograph
Gene expression protein ingredient.In entire immune response experiment, need to carry out blotting membrane the cleaning-of more test solution multifrequencies time
Incubation-cleaning operation.
Western blot test is usually that sample is placed in reaction utensil in laboratory at present, in experimentation, by test solution
It is added in reaction utensil, test solution is individually sucked out by imbibition rifle again after reaction and is cleaned, in the primary antibody examination being rejected
In liquid and secondary antibody test solution includes the antibody not being consumed largely, causes the serious waste of primary antibody and secondary antibody, and primary antibody and two
Anti- price costly, results in higher experimental cost.Therefore a kind of blotting membrane of the market demand can be actively between each test solution
Mobile immunoblotting assay device, such primary antibody and secondary antibody test solution can reuse, but wherein how realize blotting membrane
Stable holding be primary urgent need to resolve project.
Utility model content
In order to overcome the above-mentioned deficiencies of the prior art, the purpose of the utility model is to provide a kind of blotting membrane clamping positions
Device.
In order to achieve the above objectives, the technical scheme adopted by the utility model to solve the technical problem is as follows: a kind of blotting membrane
Clamping and positioning device, comprising:
Auxiliary backplane, the upper surface of the auxiliary backplane include the upper bearing surface and lower support surface with difference in height；
Clamp assemblies comprising main grip block, lower attaching plate, upper tabletting and the top board set gradually from bottom to top, institute
It states main grip block to be arranged in the lower support surface of the auxiliary backplane, upper surface and the auxiliary backplane of the main grip block
Upper bearing surface is in same plane, and blotting membrane upper end is clamped between the lower attaching plate and upper tabletting, on the main grip block
Equipped with the first positioning column and the second positioning column arranged along the lower attaching plate length direction, in the lower attaching plate and upper tabletting
Equipped with the location hole to match with first positioning column and the second positioning column.
Compared to the prior art, blotting membrane is adsorbed on lower attaching plate table by the adsorption capacity monolithic stability of moisture film to the utility model
Face, blotting membrane upper end are clamped between lower attaching plate and upper tabletting, and the part that blotting membrane enters test solution can be detached from lower attaching plate and fill
Divide and reacted with test solution, blotting membrane is detached from after test solution and meeting monolithic stability is adsorbed on lower attaching plate surface；When putting film, lower attachment
Piece, which is stablized, to be sticked on the upper surface of bearing surface and main grip block on conplane auxiliary backplane, not will cause lower attachment
The deformation of piece and blotting membrane, the first positioning column and the second positioning column can be such that lower attaching plate and blotting membrane pacifies perpendicular to main grip block
Dress, improves the position precision of blotting membrane；After the completion of blotting membrane clamping, clamp assemblies are removed from auxiliary backplane, is stablized outstanding
It is suspended on the movable plate of immunoblotting assay equipment, is moved between each test solution slot by the reaction time, carry out the cleaning-of multifrequency time
Incubation-cleaning reduces the waste of antibody test solution, has saved experimental cost, and operation is more efficient and convenient, improves experiment effect
Rate.
Further, the both ends of the main grip block are equipped with locating piece, and the auxiliary backplane is equipped with and the locating piece
The locating slot to match.
Further, position corresponding with the lower attaching plate head end is equipped with the on the upper bearing surface of the auxiliary backplane
Three positioning columns, the head end of the lower attaching plate are equipped with the semicircular opening to match with the third positioning column.
Using the above preferred scheme, the third positioning column of auxiliary backplane and first positioning column and the second positioning column are same
The setting of one straight line, respectively positions the both ends of lower attaching plate, improves the verticality essence of the relatively main grip block of lower attaching plate
Degree, it is ensured that blotting membrane high position accuracy between each test solution slot of immunoblotting assay equipment is mobile, and the arrangement of test solution slot can be more
To be compact, cavity opening be can be made smaller, and the single liquid volume added of antibody test solution is saved, and immunoblotting assay equipment can also be made
Make more small and exquisite.
Further, the lower surface of the lower support surface of the auxiliary backplane and main grip block is equipped with the opposite magnetism in position
Body mounting groove is embedded with magnetic substance in the magnetic substance mounting groove.
Using the above preferred scheme, certain the suction-combining force is kept between auxiliary backplane and main grip block, prevent slightly by
Phase shift both after power；Clamp assemblies firmly slightly can be pushed outwardly after blotting membrane clamping, improve operation ease.
It further, further include tie down screw, the tie down screw is arranged in the top board, upper tabletting and lower attaching plate
On through-hole and screw in the threaded hole on main grip block.
Using the above preferred scheme, convenient to lock each component of clamp assemblies after blotting membrane clamping, it is right
Blotting membrane provides firm chucking power.
Further, the first positioning column of multiple groups and the second positioning column are in a row equipped on the main grip block, the main folder is held
Also it is carved with the label for recognizing blotting membrane clip position in the upper surface of plate.
Using the above preferred scheme, it can be convenient and identification be marked to the multiple blotting membranes clamped on clamp assemblies,
It prevents from obscuring.
Further, it is additionally provided on the main grip block for the clamp assemblies to be hanging to immunoblotting assay equipment
On suspention ear, the suspention ear be equipped with mounting hole.
Using the above preferred scheme, clamp assemblies are improved and is installed to the convenience in immunoblotting assay equipment.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is a kind of structural schematic diagram of embodiment of the utility model；
Fig. 2 is a kind of structural schematic diagram of embodiment of the utility model；
Fig. 3 is a kind of structural schematic diagram of embodiment of the utility model；
Fig. 4 is the structural schematic diagram of immunoblotting assay equipment.
The title of number and corresponding component represented by letter in figure:
1- auxiliary backplane；The upper bearing surface of 11-；12- lower support surface；13- locating slot；14- magnetic substance mounting groove；15- third
Positioning column；2- clamp assemblies；The main grip block of 21-；The first positioning column of 211-；The second positioning column of 212-；213- locating piece；215- mark
Note；216- mounting hole；22- lower attaching plate；221- semicircular opening；The upper tabletting of 23-；24- top board；25- tie down screw；Before and after 3-
Mobile mechanism；4- reciprocating mechanism；5- movable plate；6- test solution box.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without creative efforts
Every other embodiment obtained, fall within the protection scope of the utility model.
As shown in Figure 1-3, a kind of blotting membrane clamping and positioning device, comprising:
Auxiliary backplane 1, the upper surface of auxiliary backplane 1 include the upper bearing surface 11 and lower support surface 12 with difference in height；
Clamp assemblies 2 comprising the main grip block 21 that sets gradually from bottom to top, lower attaching plate 22, upper tabletting 23 and on
Pressing plate 24, main grip block 21 are arranged in the lower support surface 12 of auxiliary backplane 1, the upper surface of main grip block 21 and auxiliary backplane 1
Upper bearing surface 11 be in same plane, blotting membrane upper end is clamped between lower attaching plate 22 and upper tabletting 24, main grip block 21
It is equipped with the first positioning column 211 arranged along 22 length direction of lower attaching plate and the second positioning column 212, lower attaching plate 22 and upper pressure
Piece 23 is equipped with the location hole to match with the first positioning column 211 and the second positioning column 212.
Beneficial effect by adopting the above technical scheme is: blotting membrane is adsorbed on lower attachment by the adsorption capacity monolithic stability of moisture film
22 surface of piece, blotting membrane upper end are clamped between lower attaching plate 22 and upper tabletting 23, and the part that blotting membrane enters test solution can be detached from
Lower attaching plate is sufficiently reacted with test solution, and blotting membrane is detached from after test solution and meeting monolithic stability is adsorbed on lower attaching plate surface；It puts
When film, lower attaching plate 22, which is stablized, to be sticked in the upper surface in bearing surface 11 and main grip block 21 on conplane auxiliary backplane
On, it not will cause the deformation of lower attaching plate 22 and blotting membrane, the first positioning column 211 and the second positioning column 212 can make lower attachment
Piece 22 and blotting membrane are installed perpendicular to main grip block 21, improve the position precision of blotting membrane.As shown in figure 4, a kind of immunoblotting
Analytical equipment, including it is moved forward and backward mechanism 3, reciprocating mechanism 4, movable plate 5 and multiple test solution boxes 6, it has been clamped in blotting membrane
Cheng Hou removes clamp assemblies 2 from auxiliary backplane 1, and stabilization is hanging on the movable plate 5 of immunoblotting assay equipment, front and back
Mobile mechanism 3 and reciprocating mechanism 4, which match, drives the blotting membrane on clamp assemblies by the reaction time between each test solution slot
It is mobile, cleaning-incubation-cleaning of multifrequency time is carried out, the waste of antibody test solution is reduced, has saved experimental cost, operation is more
It is efficient and convenient, improve conventional efficient.
As shown in Figure 1-3, the both ends of main grip block 21 are equipped with locating piece in other embodiments of the utility model
213, auxiliary backplane 1 is equipped with the locating slot 13 to match with locating piece 213；On the upper bearing surface 11 of auxiliary backplane with lower attachment
The corresponding position of piece head end is equipped with third positioning column 15, and the head end of lower attaching plate 22 is equipped with and 15 phase of third positioning column
The semicircular opening 221 matched.Beneficial effect by adopting the above technical scheme is: the third positioning column 15 of auxiliary backplane and the first positioning
Column 211 and the same straight line setting of the second positioning column 212, respectively position the both ends of lower attaching plate 22, improve lower attachment
The verticality precision of the relatively main grip block of piece, it is ensured that blotting membrane high position essence between each test solution slot of immunoblotting assay equipment
Degree movement, the arrangement of test solution slot can be more compact, and cavity opening be can be made smaller, and saves the single liquid volume added of antibody test solution,
Immunoblotting assay equipment is also made to more small and exquisite.
As shown in figure 3, in other embodiments of the utility model, the lower support surface 12 and main folder of auxiliary backplane 1
The lower surface for holding plate is equipped with the opposite magnetic substance mounting groove 14 in position, is embedded with magnetic substance in magnetic substance mounting groove 14.Using
The beneficial effect of above-mentioned technical proposal is: keeping certain the suction-combining force between auxiliary backplane and main grip block, prevents slight stress
The two phase shift afterwards；Clamp assemblies firmly slightly can be pushed outwardly after blotting membrane clamping, improve operation ease.
As shown in Figure 1, further including tie down screw 25, tie down screw is worn in other embodiments of the utility model
Through-hole in top board 24, upper tabletting 23 and lower attaching plate 22 simultaneously screws in the threaded hole on main grip block 21.Using
The beneficial effect of above-mentioned technical proposal is: it is convenient after blotting membrane clamping, each component of clamp assemblies is locked, to print
Mark film provides firm chucking power.
As shown in Fig. 2, being in a row equipped with multiple groups first on main grip block 21 in other embodiments of the utility model
The label for recognizing blotting membrane clip position is also carved in positioning column 211 and the second positioning column 212, the upper surface of main grip block 21
215.Beneficial effect by adopting the above technical scheme is: can be convenient and marks to the multiple blotting membranes clamped on clamp assemblies 2
Note identification, prevents from obscuring.
As shown in Fig. 2, being additionally provided on main grip block 21 in other embodiments of the utility model for that will clamp
Component is hanging to the suspention ear in immunoblotting assay equipment, and the suspention ear is equipped with mounting hole 216.Using above-mentioned technical side
The beneficial effect of case is: improving clamp assemblies and is installed to the convenience in immunoblotting assay equipment.
The above embodiments are only for explaining the technical ideas and features of the present invention, and its object is to allow the common skill in this field
Art personnel can understand the content of the utility model and be implemented, and not limit the protection scope of the present invention,
All equivalent change or modifications according to made by the spirit of the present invention essence, should all cover in the protection scope of the utility model
It is interior.
Claims (7)
1. a kind of blotting membrane clamping and positioning device characterized by comprising
Auxiliary backplane, the upper surface of the auxiliary backplane include the upper bearing surface and lower support surface with difference in height；
Clamp assemblies comprising main grip block, lower attaching plate, upper tabletting and the top board set gradually from bottom to top, the master
Grip block is arranged in the lower support surface of the auxiliary backplane, the upper surface of the main grip block and the upper branch of the auxiliary backplane
Bearing surface is in same plane, and blotting membrane is integrally adsorbed on lower attaching plate surface, blotting membrane upper end be clamped in the lower attaching plate and
Between upper tabletting, the main grip block is equipped with the first positioning column arranged along the lower attaching plate length direction and the second positioning
Column, the lower attaching plate and upper tabletting are equipped with the location hole to match with first positioning column and the second positioning column.
2. blotting membrane clamping and positioning device according to claim 1, which is characterized in that the both ends of the main grip block are equipped with
Locating piece, the auxiliary backplane are equipped with the locating slot to match with the locating piece.
3. blotting membrane clamping and positioning device according to claim 2, which is characterized in that the upper bearing surface of the auxiliary backplane
Upper position corresponding with the lower attaching plate head end is equipped with third positioning column, and the head end of the lower attaching plate is equipped with described the
The semicircular opening that three positioning columns match.
4. blotting membrane clamping and positioning device according to claim 3, which is characterized in that the lower support surface of the auxiliary backplane
It is equipped with the opposite magnetic substance mounting groove in position with the lower surface of main grip block, is embedded with magnetism in the magnetic substance mounting groove
Body.
5. blotting membrane clamping and positioning device according to claim 4, which is characterized in that it further include tie down screw, the lock
Tight screw rod is arranged in the top board, upper tabletting and through-hole in lower attaching plate and screws in the threaded hole on main grip block.
6. blotting membrane clamping and positioning device according to claim 5, which is characterized in that be in a row equipped on the main grip block
It is also carved with for recognizing blotting membrane clip position the upper surface of the first positioning column of multiple groups and the second positioning column, the main grip block
Label.
7. blotting membrane clamping and positioning device according to claim 6, which is characterized in that be additionally provided with use on the main grip block
In the suspention ear being hanging to the clamp assemblies in immunoblotting assay equipment, the suspention ear is equipped with mounting hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821992313.XU CN209215404U (en) | 2018-11-29 | 2018-11-29 | A kind of blotting membrane clamping and positioning device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821992313.XU CN209215404U (en) | 2018-11-29 | 2018-11-29 | A kind of blotting membrane clamping and positioning device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209215404U true CN209215404U (en) | 2019-08-06 |
Family
ID=67462093
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821992313.XU Active CN209215404U (en) | 2018-11-29 | 2018-11-29 | A kind of blotting membrane clamping and positioning device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209215404U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111412860A (en) * | 2020-03-25 | 2020-07-14 | 广东省微生物研究所（广东省微生物分析检测中心） | Observation objective table for laser confocal microscope biofilm |
| CN114264806A (en) * | 2021-12-16 | 2022-04-01 | 苏州邦器生物技术有限公司 | Membrane strip for immunoblotting and manufacturing method thereof |
-
2018
- 2018-11-29 CN CN201821992313.XU patent/CN209215404U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111412860A (en) * | 2020-03-25 | 2020-07-14 | 广东省微生物研究所（广东省微生物分析检测中心） | Observation objective table for laser confocal microscope biofilm |
| CN114264806A (en) * | 2021-12-16 | 2022-04-01 | 苏州邦器生物技术有限公司 | Membrane strip for immunoblotting and manufacturing method thereof |
| CN114264806B (en) * | 2021-12-16 | 2024-03-29 | 苏州邦器生物技术有限公司 | Membrane strip for immunoblotting and manufacturing method thereof |
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| TR01 | Transfer of patent right |
Effective date of registration: 20210713 Address after: Floor 1-5, building 12, phase 3-1, Wuhan Optics Valley International Biomedical enterprise accelerator, 388 Gaoxin 2nd Road, Donghu New Technology Development Zone, Wuhan City, Hubei Province, 430000 Patentee after: Mona (Wuhan) Biotechnology Co.,Ltd. Address before: 222000 12-14 / F, building 1, Kechuang City, No.17, Huaguoshan Avenue, Haizhou District, Lianyungang City, Jiangsu Province Patentee before: MONAD (LIANYUNGANG) BIOTECHNOLOGY Co.,Ltd. |