CN217479477U - Cell culture micropore plate - Google Patents

Cell culture micropore plate Download PDF

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Publication number
CN217479477U
CN217479477U CN202122255217.5U CN202122255217U CN217479477U CN 217479477 U CN217479477 U CN 217479477U CN 202122255217 U CN202122255217 U CN 202122255217U CN 217479477 U CN217479477 U CN 217479477U
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China
Prior art keywords
vernier
frame
bottom plate
cell culture
sample
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CN202122255217.5U
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Chinese (zh)
Inventor
施荣华
周典法
何海辉
凌聪
张海燕
张瑞龙
吴高
丁运生
王振洋
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University of Science and Technology of China USTC
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University of Science and Technology of China USTC
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Abstract

The utility model discloses a cell culture micropore board, the bottom of frame has the bottom plate, and the vernier is installed to the upside of frame, and the tip of vernier passes through the magnetic path to be supported in bottom plate and sliding fit, and the magnetic stripe that attracts the magnetic path is installed to the bottom plate, and the vernier that slides along the vernier is installed to the vernier. Install the gliding vernier of edge frame at the frame upside, the vernier is the strip, cover whole frame, the magnetic path through the tip supports in the bottom plate, the magnetic stripe magnetic path has the effect of inter attraction simultaneously, when sliding to fixed position, be difficult to take place the offset, the vernier is used for fixing a position horizontal position, the vernier is used for fixing a position longitudinal position, move the vernier to the position in next application of sample hole according to the order of application of sample, then remove the vernier, remove the vernier to next application of sample hole after the application of sample, avoid makeing mistakes.

Description

Cell culture micropore plate
Technical Field
The utility model belongs to the technical field of cell detection equipment, especially, relate to a cell culture micropore board.
Background
An extracellular flow meter is the most advanced device for measuring cell energy metabolism. The instrument is designed by a special probe plate and a cell culture micro-porous plate, is controlled by a preset program within a specific time, adds up to four medicaments from the probe plate to a culture medium in a cell plate to be detected, and synchronously detects dissolved oxygen variation (OCR) and pH value variation (ECAR) in real time by utilizing a non-invasive patent optical sensor in a microenvironment of about 2.2-5.6ul temporarily formed during measurement, so that the energy metabolism state of two large energy conversion paths (aerobic metabolism and glycolysis of mitochondria) in cells is quickly known. The method has the advantages of rapid and accurate test of metabolic indexes such as cell basal metabolic rate, ATP conversion, membrane integrity, limiting respiration rate, mitochondrial function, oxygen radical generation and the like, time and labor are saved, and experimental data are more scientific and convincing.
The XF96 model instrument is the one with the largest detection flux at present, and comprises a 96-hole cell culture micro-porous plate and a probe plate which are both made of polystyrene materials. The cell culture micro-porous plate is used for culturing cells, the cells are subjected to drug tests or other tests, the influence of the drug on the cell metabolism needs to be proved, and a culture solution containing the cells is added when the cell culture micro-porous plate is used. And probe card and micropore board cooperation are used, and mutual block from top to bottom, probe card include the frame, and the embedded optical fiber post that has of frame, optical fiber post and cell application of sample hole one-to-one, the avris of optical fiber post has the ware of pounding medicine, and the ware of pounding medicine is four evenly distributed in the optical fiber post, and its bottom has the hole, adds inhibitor, coupling agent and irritant etc. according to the demand during the use, tests the influence that produces to cell culture. The fiber column is hollow and designed for fiber insertion, and the bottom seal is used for smearing the probe and contacting with cell fluid. And after the detection, putting the micro-porous plate and the probe plate which are covered together into a data analyzer for detection.
The current 96 hole cell culture micropore boards, the quantity in hole is many, when the injection contains the nutrient solution of cell, need align the hole then squeeze the liquid rifle, and the cell sap is many all transparent, it has been injected into the nutrient solution to hardly judge which hole, which does not, though the avris of board has capital letter and figure and carries out the joint coordinate, but the coordinate efficiency will be checked to application of sample at every turn all is very low, the thought is not concentrated very easily interrupted simultaneously, forget the application of sample coordinate, will detect again and ensure the accuracy. Therefore, the utility model discloses it is many that the main solution adds the appearance hole quantity, and it is dazzling easily, and checks the coordinate and carry out the application of sample and lead to the problem that efficiency is very low.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a cell culture micropore board, what main solution is that the application of sample hole is in large quantity, and is dazzling easily, and checks the coordinate and carries out the application of sample and lead to the problem that efficiency is very low.
In order to solve the technical problem, the utility model discloses a realize through following technical scheme:
a cell culture microporous plate comprises a rectangular frame, wherein a plurality of sampling holes are arranged and fixed in the frame, and the sampling holes are arranged in a dot matrix manner and are fixed with each other;
the bottom of the frame is provided with a bottom plate which is in a square frame shape;
the upper side of the frame is provided with a vernier, the side projection of the vernier is a shape of a triangle 21274, the vernier is clamped on the frame, the end part of the vernier is supported on the bottom plate through a magnetic block and is in sliding fit with the bottom plate, and the bottom plate is provided with a magnetic strip for attracting the magnetic block;
the vernier which slides along the vernier is arranged on the vernier.
Furthermore, the bottom plate is provided with a groove body, and the magnetic strip is detachably arranged on the groove body.
Furthermore, the bottom plate is provided with a silica gel pad for sealing the groove body, and the silica gel pad is fixed on the magnetic strip.
Furthermore, a sliding groove for sliding the vernier is formed in the upper side of the vernier.
Furthermore, a reinforcing rib is arranged between the sample adding hole and the frame.
The utility model discloses following beneficial effect has:
(1) install the gliding vernier of edge frame at the frame upside, the vernier is the strip, covers whole frame, and the magnetic path through the tip supports in the bottom plate, and the magnetic stripe magnetic path has the effect of inter attraction simultaneously, slides when fixed position, is difficult to take place the offset, and the vernier is used for fixing a position horizontal position, and the vernier is used for fixing a position vertical position.
(2) Remove the vernier to the position of next application of sample hole according to the order of application of sample, then remove the vernier, remove the vernier to next application of sample hole behind the application of sample, avoid makeing mistakes, product overall dimension is the palm size, and vernier all can be stirred through the finger, and the one-hand does not need extra complicated operation alright completion even.
Of course, it is not necessary for any particular product to achieve all of the above-described advantages at the same time.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments are briefly introduced below, it is obvious that the drawings in the description below are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1: the utility model discloses structure chart upper portion.
FIG. 2: the utility model discloses the structure chart bottom.
FIG. 3: the utility model discloses the structure chart plan view.
In the drawings, the components represented by the respective reference numerals are listed below:
the frame 1, the sampling hole 11, the bottom plate 2, the vernier 3, the magnetic block 31, the magnetic stripe 22, the vernier 32, the groove body 21, the silica gel pad 23, the chute 33 and the reinforcing rib 12.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "open hole", "upper", "lower", "thickness", "top", "middle", "length", "inner", "around", etc. indicate an orientation or positional relationship only for the convenience of description of the present invention and to simplify the description, and do not indicate or imply that the component or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
As shown in fig. 1-3: a cell culture microporous plate comprises a rectangular frame 1, wherein a plurality of sampling holes 11 are arranged and fixed in the frame 1, and the sampling holes 11 are arranged in a dot matrix manner and are mutually fixed;
the bottom of the frame 1 is provided with a bottom plate 2, and the bottom plate 2 is in a square frame shape;
the upper side of the frame 1 is provided with a vernier 3, the side projection of the vernier 3 is \ 21274, the vernier is clamped on the frame 1, the end part of the vernier 3 is supported on the bottom plate 2 through a magnetic block 31 and is in sliding fit with the bottom plate 2, and the bottom plate 2 is provided with a magnetic strip 22 for attracting the magnetic block 31;
the vernier 32 is mounted on the vernier 3 to slide along the vernier 3.
The utility model discloses in, install along the gliding vernier of frame at the frame upside, the vernier is the strip, covers whole frame, and the magnetic path through the tip supports in the bottom plate, and the magnetic stripe magnetic path has the effect of inter attraction simultaneously, slides when fixing a position, is difficult to take place the offset, and the vernier is used for fixing a position horizontal position, and the vernier is used for fixing a position vertical position.
Remove the vernier to the position of next application of sample hole according to the order of application of sample, then remove the vernier, remove the vernier to next application of sample hole after the application of sample, avoid makeing mistakes, product overall dimension is the palm size, and vernier all can be stirred through the finger, and the one hand does not need extra complicated operation alright completion even.
As shown in fig. 1-2: the bottom plate 2 is provided with a groove body 21, and the magnetic strip 22 is detachably mounted on the groove body 21. Magnetic stripe and vernier are detachable installation, and the micropore board product is disposable product, in order to avoid extravagant, magnetic stripe and vernier are to the micropore board repeatedly usable of same size.
As shown in fig. 1: the bottom plate 2 is provided with a silica gel pad 23 for sealing the groove body 21, and the silica gel pad 23 is fixed on the magnetic strip 22. The silica gel pad is used for sealing, improves the frictional force with the mesa simultaneously, reduces the risk of whole product micropore board landing. The magnetic strip and the magnetic strip are embedded into the groove body as a whole.
As shown in fig. 1: the upper side of the vernier 3 is provided with a sliding groove 33 for sliding the vernier 32.
As shown in fig. 2: and a reinforcing rib 12 is arranged between the sampling hole 11 and the frame 1.
In the description of the present specification, reference to the description of "one embodiment," "an example," "a specific example," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and its practical applications, to thereby enable others skilled in the art to best understand the invention for and utilize the invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims (5)

1. A cell culture microporous plate comprises a rectangular frame (1), wherein a plurality of sampling holes (11) are arranged and fixed in the frame (1), and the sampling holes (11) are arranged in a dot matrix manner and are mutually fixed; the method is characterized in that:
the bottom of the frame (1) is provided with a bottom plate (2), and the bottom plate (2) is in a square frame shape;
the upper side of the frame (1) is provided with a vernier (3), the side projection of the vernier (3) is gamma 21274, the vernier is clamped on the frame (1), the end part of the vernier (3) is supported on the bottom plate (2) through a magnetic block (31) and is in sliding fit with the bottom plate, and the bottom plate (2) is provided with a magnetic strip (22) for attracting the magnetic block (31);
the vernier (32) sliding along the vernier (3) is installed on the vernier (3).
2. A cell culture microplate according to claim 1, wherein: the bottom plate (2) is provided with a groove body (21), and the magnetic strip (22) is detachably arranged on the groove body (21).
3. A cell culture microplate according to claim 2, wherein: the bottom plate (2) is provided with a silica gel pad (23) of the sealing groove body (21), and the silica gel pad (23) is fixed on the magnetic strip (22).
4. A cell culture microplate according to claim 1, wherein: the upper side of the vernier (3) is provided with a sliding groove (33) for the vernier (32) to slide.
5. A cell culture microplate according to claim 1, wherein: and a reinforcing rib (12) is arranged between the sampling hole (11) and the frame (1).
CN202122255217.5U 2021-09-16 2021-09-16 Cell culture micropore plate Active CN217479477U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202122255217.5U CN217479477U (en) 2021-09-16 2021-09-16 Cell culture micropore plate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202122255217.5U CN217479477U (en) 2021-09-16 2021-09-16 Cell culture micropore plate

Publications (1)

Publication Number Publication Date
CN217479477U true CN217479477U (en) 2022-09-23

Family

ID=83302010

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202122255217.5U Active CN217479477U (en) 2021-09-16 2021-09-16 Cell culture micropore plate

Country Status (1)

Country Link
CN (1) CN217479477U (en)

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