CN203551564U

CN203551564U - Manual sample feeding auxiliary device of ELISA (Enzyme Linked Immunosorbent Assay) micro-porous plate

Info

Publication number: CN203551564U
Application number: CN201320538617.XU
Authority: CN
Inventors: 马展; 陈黎; 张泓
Original assignee: Shanghai City Children Hospital
Current assignee: Shanghai City Children Hospital
Priority date: 2013-08-30
Filing date: 2013-08-30
Publication date: 2014-04-16
Anticipated expiration: 2023-08-30

Abstract

The utility model provides a sampling auxiliary system. An enzyme linked immunosorbent assay (ELISA) is widely applied to the detection of antigens or antibodies; a present-stage ELISA is mainly carried out in a manual manner; under the condition that a plurality of projects and a plurality of micro-porous plates are carried out at the same time, a sampling step is complicated, much time is consumed and the error is easy to make, and the sampling speed is slow to cause that the experiment batch is increased and the complexity of experiments is also increased. The sampling auxiliary system comprises a transparent tabletop (3); positions for placing a deep hole diluting plate and a plurality of ELISA plates are integrated above the transparent tabletop (3); a straight ruler (1) capable of sliding on a slide rail (2) is mounted above the ELISA plates; a replaceable lining plate (5) printed with a sampling scheme is arranged below the transparent tabletop. Due to the adoption of the system, the sampling efficiency can be obviously improved; the experiment batch is reduced, the flow is smooth and the sampling error is reduced.

Description

The manual application of sample servicing unit of a kind of enzyme mark microwell plate

Technical field

The utility model relates to a kind of enzyme mark microwell plate plurality of plates carries out the servicing unit of manual application of sample simultaneously, for improving the efficiency of enzyme mark microwell plate loading, and prevents the generation of mistake.

Background technology

Enzyme linked immunosorbent assay (ELISA) is as a kind of method of measuring trace antigen, antibody, sensitive, the special and low cost with it and be widely used in scientific research and clinical assays, especially at clinical medicine clinical labororatory (hospital laboratory), ELISA is extensively carried out as the most conventional technology of immunological test.

ELISA carries out with batch sequential mode conventionally on microwell plate, and its basic process comprises application of sample, and sample is hatched, and washes for the first time version, and version is hatched, washed for the second time to enzyme conjugates, and substrate colour developing, reads the several processes of plate.Although there has been full-automatic instrument to emerge, but due to the intrinsic batch sequence pattern restriction of ELISA, especially when multiple projects, polylith version are carried out simultaneously (this is very common in clinical laboratory's routine work) full-automatic instrument efficiency not high, even also slow than manual in some situation, therefore manual ELISA operation is inevitable.

When multiple projects, polylith version are carried out simultaneously, manual ELISA is a labor intensive, especially application of sample step, sample need be gone in corresponding each hole that joins 96 hole microwell plates one by one, while carrying out multiple projects, plurality of plates at the same time, this work will be loaded down with trivial details and fallibility very; Because the application of sample time is oversize, also will cause that between different holes, excessive the causing between unacceptable hole of incubation time difference makes a variation, and causes the insincere of result simultaneously.

Take common prenatal and postnatal care inspection item (ToRCH) as example, ToRCH comprises altogether 10 projects, take every batch of 92 each samples as example, so just there are 10 blocks of plates, comprise contrast and blank 960 holes altogether, according to normal application of sample speed, within 8-10 minute, add 1 plate meter, for fear of differing from 10 blocks of plates between excessive hole, will have to be divided at least 5 batches, whole application of sample process will consume nearly 2 hours, due to 5 batches, interlock and carry out simultaneously simultaneously, very in a rush of operator, " dead time " that between considering batch, time conflict produces, complete 2 people of whole common needs continuous 4 hours or 1 people uninterruptedly carries out for continuous 7 hours, moreover in the face of 96 orifice plate operations can cause operator's spirit high-pressure, very easily there is application of sample mistake in long-time continuous.

Therefore, improve application of sample speed and prevent application of sample mistake become multiple projects, polylith version carry out simultaneously ELISA measure time significant problem.For the existing solution of this problem, be to use multi-channel loading robot (or claiming liquid working station), by many sample needles, work simultaneously and can make this problem be solved, but the huge volume of multi-channel loading robot and easily prices hundreds thousand of, units up to a million make a lot of laboratories hang back.

Summary of the invention

The utility model provides a kind of enzyme mark microwell plate plurality of plates to carry out the servicing unit of manual application of sample simultaneously, the structure of this device comprises a transparent table top, table top comprises that one is placed the high-rise base plate of microwell plate and the low layer base plate of a placement deep-well plates, the grid of restriction microwell plate position is installed on table top, above microwell plate and high laminate, have a ruler that can slide along slide rail, the below of base plate has one because application of sample scheme is different, can extract liner plate replacement, that be printed on application of sample scheme out.Collaborative by deep-well plates, Multi-channel liquid transfer device, application of sample scheme liner plate, ruler, thereby the speed of application of sample raising loading reduce mistake and realize at the Fast synchronization application of sample that there is no polylith microwell plate under application of sample robot condition when realizing 8-12 hole.

Accompanying drawing explanation

Fig. 1 top view

Fig. 2 front elevation

Fig. 3 side view

The positive middle section figure of Fig. 4

Fig. 5. the part that is printed on the liner plate of application of sample scheme and has amplified

Caption:

1. be arranged on the collimation chi on slide rail (2.)

2. be arranged on the slide rail on the upper lower edge chimb of table top (3.)

3. transparent organic glass table top

4. place the bottom base plate of deep-well plates

5. application of sample scheme liner plate

6. on base plate for the spacing grid of ELISA Plate

7. place the high-rise base plate of normal enzyme target

Embodiment

The design is as shown in the figure: native system main body be the work top made of an organic glass 3., comprise that 4. base plate that 1 not contour transparent organic glass makes (places the low layer base plate of deep-well plates) and 7. with grid, 6. separate 12 plate positions just in time putting into a microwell plate on (the high-rise base plate of placement microwell plate), base plate, wherein 2, the lower right corner holds the deep-well plates of reagent and sample for placing consumption, is lined with a liner plate that is printed on hole number and application of sample scheme 5. under base plate.3. the upper of work top respectively have a slide rail below, and collimation chi two ends 1. can be fixed on slide rail and move horizontally.

Compared with conventional method, the difference of using this device to carry out E LISA test is embodied in the pre-dilution of sample and loading step:

1, sample dilutes in advance:

Because deep-well plates provides the pitch-row identical with multi-channel loading device and microwell plate, while using native system, in the test tube that is diluted in advance deep-well plates rather than routine of sample, carry out.The deep-well plates position that deep-well plates is placed on worktable 4., during original serum application of sample, see through at the bottom of transparent deep-well plates and base plate, the application of sample scheme that 5. printing liner plate is gone up is high-visible, under the indication of liner plate, each hole of microwell plate is indicated by clear, 1. the collimation chi that simultaneously slides arrive that row of application of sample, and under the assisting of collimation chi and application of sample scheme, the possibility that original sample adds wrong hole is greatly diminished.

Because the design of deep-well plates is originally used for keeping sample rather than diluting use, cannot shake and mix, for solving dilution, mix problem, this device should adopt in use and first add the scheme that adds dilution after sample, utilizes dilution and square hole deep hole wooden partition to impact the eddy current producing and completes dilution and mix.

2, the synchronous loading of many plates

Because deep-well plates, ELISA Plate, multi-channel loading utensil have identical pitch-row, therefore in loading link, use multi-channel loading device can once complete the synchronous application of sample in 8-12 hole, loading speed increases several times, because the parallel application of sample of the increase polylith microwell plate of loading speed is achieved, 10 microwell plates can carry out in one batch.The use that simultaneously collimates chi and liner plate can reduce the probability that adds wrong hole greatly.

3, subsequent step

Consistent with traditional E LISA experimental technique.

Claims

1. the manual application of sample servicing unit of enzyme mark microwell plate, collaborative by deep-well plates, Multi-channel liquid transfer device, application of sample scheme liner plate, collimation chi, improve the speed of loading and reduce error rate, the structure of this device comprises a transparent table top, table top comprises that one is placed the high-rise base plate of microwell plate and the low layer base plate of a placement deep-well plates, the grid of restriction microwell plate position is installed on table top, above microwell plate and high laminate, have a ruler that can slide along slide rail, there is a liner plate that is printed on application of sample scheme the below of base plate.

2. the manual application of sample servicing unit of enzyme mark microwell plate according to claim 1, is characterized in that, its liner plate that is printed on application of sample scheme is removable.