CN203949924U - For the biochip of quick magnetic immunoassay - Google Patents
For the biochip of quick magnetic immunoassay Download PDFInfo
- Publication number
- CN203949924U CN203949924U CN201420137862.4U CN201420137862U CN203949924U CN 203949924 U CN203949924 U CN 203949924U CN 201420137862 U CN201420137862 U CN 201420137862U CN 203949924 U CN203949924 U CN 203949924U
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- CN
- China
- Prior art keywords
- biochip
- kapillary
- quick
- framework
- adsorptive pads
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000018 DNA microarray Methods 0.000 title claims abstract description 28
- 238000003018 immunoassay Methods 0.000 title claims abstract description 21
- 230000000274 adsorptive effect Effects 0.000 claims abstract description 29
- 239000000463 material Substances 0.000 claims description 5
- 229920002678 cellulose Polymers 0.000 claims description 3
- 239000001913 cellulose Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 2
- 239000011347 resin Substances 0.000 claims description 2
- 239000000470 constituent Substances 0.000 claims 3
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims 1
- -1 polyoxyethylene Polymers 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 abstract 2
- 238000004458 analytical method Methods 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 8
- 239000012530 fluid Substances 0.000 description 6
- 238000003745 diagnosis Methods 0.000 description 5
- 239000011521 glass Substances 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000001259 photo etching Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 1
- 206010019280 Heart failures Diseases 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 230000005415 magnetization Effects 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
Abstract
The utility model provides a kind of biochip for quick magnetic immunoassay, it is characterized in that, comprise biochip framework, and be set in turn in sample cell, kapillary, the adsorptive pads of framework top, and being arranged at the magnetic devices outside framework, described two ends capillaceous connect respectively sample cell and adsorptive pads.The utility model is easy to use, and analyzing and testing is rapid, highly sensitive, is particularly suitable for the express-analysis of biological sample.
Description
Technical field
The utility model belongs to medical science quick diagnosis detection technique field, particularly a kind of biochip for quick magnetic immunoassay.
Background technology
Immunologic assay is taking the specific recognition of antibody-antigen, antibody labeling technology and tracer technique as basic quantitative technique.Existing clinical in vitro diagnosis in vitro has radio immunoassay, enzyme-linked immunosorbent assay, time resolved fluoro-immunoassay method, chemiluminometry etc.But in actual immune detection, because impurities composition in testing sample is more, detection sensitivity and accuracy are affected to a certain extent, so separate fast, be purified into object determinand from complicated sample substrate, it is the difficult problem that clinical examination worker faces, during in particular for quick diagnosis field, this problem is especially outstanding.
Utility model content
The utility model provides a kind of biochip for quick magnetic immunoassay, and its device is simple, light, highly sensitive, can meet the requirement of external quick diagnosis.
The utility model for the biochip of quick magnetic immunoassay, comprises biochip framework, and is set in turn in sample cell, kapillary and adsorptive pads on framework, and is arranged at the magnetic devices in framework outside.
Described sample cell is that framework upper surface forms the groove with a constant volume, is formed by photoetching or etching.
Described kapillary is the microfluidic channel being formed by glass that connects sample cell and adsorptive pads, can be linear structure, or curve construction; Can be the single channel that single capillary forms, in the time that needs detect multiple parameter simultaneously, the hyperchannel that can be made up of side by side many capillaries, to reach the object that detects multiple projects simultaneously.
Described adsorptive pads can be the material composition that cellulose or other have planar water, as filter paper, as cellulose filter paper or artificial synthetic water-absorbing resins.
Described magnetic devices can be external magnet, for being placed in the magnet of framework one side or below, can be also the magnetic device of other tools, as the detection system with magnet.
Kapillary of the present utility model can be drawn to adsorptive pads by capillary force by the fluid sample in sample cell, and then adsorptive pads can accelerate again the sample flow in sample cell.
As accessible in persons skilled in the art, when test, to be added drop-wise in sample cell through the fluid sample of magnetization immune-treated, under the magnetic field producing at magnetic devices and capillary action double action capillaceous, move to adsorptive pads direction, and magnetic bead in fluid sample concentrates and be fixed on magnetic field detection region, then be combined with luminous or fluorescence immunoassay detection technique, can greatly improve detection sensitivity and accuracy, because of the use of magnetic particle carrier technique, detection time can obviously be shortened simultaneously.
The utility model can support serial external diagnosis reagent case to comprise the kits such as myocardial infarction mark reagents series box, reagents series box in heart failure and inflammatory factor biomarker series.
Below by the drawings and specific embodiments, the utility model is described in further detail, and embodiment provides with the object of giving an example, and should understand and not form any restriction of the present utility model.
Brief description of the drawings
Fig. 1 is the utility model structural representation Fig. 1
Fig. 2 is that the utility model detects schematic diagram 2
Fig. 3 is the utility model structural representation Fig. 3
Wherein, 1 is biological chip frame, and 2 is sample cell, and 3 is kapillary, and 4 is adsorptive pads, and 5 is magnetic devices
Embodiment
Embodiment 1
As accompanying drawing 1, the utility model, for the biochip of quick magnetic immunoassay, comprises biochip framework 1, and is set in turn in sample cell 2, kapillary 3 and adsorptive pads 4 on framework, and be arranged at the magnetic devices 5 of framework one side.Sample cell 2 is by photoetching or being etched in framework upper surface forms the groove with a constant volume; Kapillary 3 is the microfluidic channels that formed by glass that connect sample cell and adsorptive pads, and in the present embodiment, kapillary 3 is linear structure.Adsorptive pads 4 adopts filter paper as absorbent material.Kapillary 3 can be drawn to adsorptive pads 4 by capillary force by the fluid sample in sample cell 2, and then adsorptive pads 4 can accelerate again the sample flow in sample cell.Magnetic devices 5 in the present embodiment is for being placed in the magnet of biochip framework 1 one sides.
Embodiment 2
As accompanying drawing 2, the utility model, for the biochip of quick magnetic immunoassay, comprises biochip framework 1, and is set in turn in sample cell 2, kapillary 3 and adsorptive pads 4 on framework, and be arranged at the magnetic devices 5 of frame underneath.Sample cell 2 is to be formed the groove with a constant volume at framework upper surface by photoetching; Kapillary 3 is the microfluidic channels that formed by glass that connect sample cell and adsorptive pads, and in the present embodiment, kapillary 3 is curve construction.Adsorptive pads 4 is filter paper.Kapillary 3 can be drawn to adsorptive pads 4 by capillary force by the fluid sample in sample cell 2, and then adsorptive pads 4 can accelerate again the sample flow in sample cell.Magnetic devices 5 in the present embodiment is for being placed in the magnet of biochip framework 1 below.
Embodiment 3
As accompanying drawing 3, the utility model, for the biochip of quick magnetic immunoassay, comprises biochip framework 1, and is set in turn in sample cell 2, kapillary 3 and adsorptive pads 4 on framework, and be arranged at the magnetic devices 5 of frame underneath.Sample cell 2 is to form the groove with a constant volume by being etched in framework upper surface; Kapillary 3 is the microfluidic channels that formed by glass that connect sample cell and adsorptive pads, and in the present embodiment, kapillary 3 is the kapillary group that three capillaries form, and can be used for measuring three parameters simultaneously.Adsorptive pads 4 is filter paper.Kapillary 3 can be drawn to adsorptive pads 4 by capillary force by the fluid sample in sample cell 2, and then adsorptive pads 4 can accelerate again the sample flow in sample cell.Magnetic devices 5 in the present embodiment is for being placed in the magnet of biochip framework 1 below.
Claims (10)
1. for the quick biochip of magnetic immunoassay, it is characterized in that, comprise biochip framework, and be set in turn in sample cell, kapillary, the adsorptive pads of framework top, and being arranged at the magnetic devices outside framework, described two ends capillaceous connect respectively sample cell and adsorptive pads.
2. the described biochip for quick magnetic immunoassay as claimed in claim 1, is characterized in that, described sample cell is the groove with a constant volume of framework upper surface.
3. the described biochip for quick magnetic immunoassay as claimed in claim 1, is characterized in that, described kapillary is straight line kapillary.
4. the described biochip for quick magnetic immunoassay as claimed in claim 1, is characterized in that, described kapillary is curve kapillary.
5. the described biochip for quick magnetic immunoassay as claimed in claim 1, is characterized in that, described kapillary is the kapillary groups that many capillaries form side by side.
6. the described biochip for quick magnetic immunoassay as claimed in claim 1, is characterized in that, the constituent material of described adsorptive pads is selected from cellulose filter paper.
7. the described biochip for quick magnetic immunoassay as claimed in claim 1, is characterized in that, the constituent material of described adsorptive pads is selected from artificial synthetic water-absorbing resins.
8. the described biochip for quick magnetic immunoassay as claimed in claim 1, is characterized in that, the constituent material of described adsorptive pads is selected from polyoxyethylene series of products.
9. the described biochip for quick magnetic immunoassay as claimed in claim 1, is characterized in that, described magnetic devices is the magnet that is placed in frame underneath.
10. the described biochip for quick magnetic immunoassay as claimed in claim 1, is characterized in that, described magnetic devices is the magnet that is placed in framework one side.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420137862.4U CN203949924U (en) | 2014-03-25 | 2014-03-25 | For the biochip of quick magnetic immunoassay |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420137862.4U CN203949924U (en) | 2014-03-25 | 2014-03-25 | For the biochip of quick magnetic immunoassay |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203949924U true CN203949924U (en) | 2014-11-19 |
Family
ID=51891953
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420137862.4U Expired - Lifetime CN203949924U (en) | 2014-03-25 | 2014-03-25 | For the biochip of quick magnetic immunoassay |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203949924U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104880562A (en) * | 2015-04-08 | 2015-09-02 | 上海盛复源生物医药有限公司 | L-FABP (Liver-Fatty Acid Binding Protein) rapid detection reagent and preparation method thereof |
| CN106053455A (en) * | 2016-05-30 | 2016-10-26 | 江苏微全芯生物科技有限公司 | Thread chip and processing technique and processing device thereof |
| CN109521196A (en) * | 2018-12-27 | 2019-03-26 | 正元盛邦(天津)生物科技有限公司 | A kind of magnetic test strips of chlamydia pneumoniae (cp) detection and corresponding paper box |
-
2014
- 2014-03-25 CN CN201420137862.4U patent/CN203949924U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104880562A (en) * | 2015-04-08 | 2015-09-02 | 上海盛复源生物医药有限公司 | L-FABP (Liver-Fatty Acid Binding Protein) rapid detection reagent and preparation method thereof |
| CN106053455A (en) * | 2016-05-30 | 2016-10-26 | 江苏微全芯生物科技有限公司 | Thread chip and processing technique and processing device thereof |
| CN109521196A (en) * | 2018-12-27 | 2019-03-26 | 正元盛邦(天津)生物科技有限公司 | A kind of magnetic test strips of chlamydia pneumoniae (cp) detection and corresponding paper box |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 201321, Lane 3399, Lane 6, Kang Xin Road, Shanghai, Pudong New Area Patentee after: SHANGHAI ZHICHENG BIOLOGICAL TECHNOLOGY CO.,LTD. Address before: 201321, Lane 3399, Lane 6, Kang Xin Road, Shanghai, Pudong New Area Patentee before: Shanghai Zhicheng Biological Technology Co.,Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20141119 |