CN204964518U - Antibody interact's quick detection device - Google Patents
Antibody interact's quick detection device Download PDFInfo
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- CN204964518U CN204964518U CN201520704046.1U CN201520704046U CN204964518U CN 204964518 U CN204964518 U CN 204964518U CN 201520704046 U CN201520704046 U CN 201520704046U CN 204964518 U CN204964518 U CN 204964518U
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- antibody
- antibody response
- aseptic
- monitoring pipe
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Abstract
The utility model discloses an antibody interact's quick detection device, including cylindrical bracket and antibody response monitoring pipe, antibody reaction unit fixes in the inside of antibody response detecting tube body, and antibody reaction unit and aseptic drainage tube UNICOM, the inside at antibody response detecting tube body is fixed to antibody immunodetection magnetism post, antibody immunodetection magnetism post and aseptic rubber tube interface UNICOM. This antibody interact's quick detection device, multiunit antibody response monitoring pipe is parallelly connected to be arranged through setting up, the interact and the detection of can the concurrent operation different antibody and antigen, and antibody reaction unit is through aseptic drainage tube and antibody immunodetection magnetism post UNICOM, and set up the inside at antibody response detecting tube body, not only can prevent external environment's interference, and also can not the mutual interference between antibody response monitoring pipe and the antibody response monitoring pipe, consequently, the degree of accuracy of detection speed and detection can improve.
Description
Technical field
The utility model belongs to biological antibody detection technique field, is specifically related to the interactional device for fast detecting of a kind of antibody.
Background technology
Enzyme linked immunosorbent assay (being called for short ELISA), was widely used in immunology and biochemical field by since Engvall and Perlman report from 1971.Along with the continuous renewal of the updating of method, microplate reader, in the context of detection of antigen and antibody, ELISA be still current test in laboratory analyze in one of the most frequently used technology.The ultimate principle of ELISA method, after antigen and antibody molecule covalent bond, does not affect respective immunologic competence.By antigen coated, close, add primary antibodie, add ELIAS secondary antibody, the step such as substrate colour developing, judge the specificity of antigen and antibody response, by the depth determination antibody of color reaction or the concentration of antigen.This kind of chromogenic reaction carries out quantitative measurement by ELISA detector (microplate reader), makes the detection method that ELISA method becomes a kind of not only special but also responsive.But ELISA reaction, from antigen coated to substrate colour developing, need multiple step, needs within 4-6 hour, even spend the night in time.
Therefore, in order to solve the shortcoming of ELISA reaction time length, the low sensitivity issues usually occurred in immunochromatography can be solved again simultaneously, needing to design the interactional device for fast detecting of a kind of antibody and solving the above-mentioned problem mentioned.
Utility model content
The purpose of this utility model is to provide a kind of antibody interactional device for fast detecting, to solve the problem proposed in above-mentioned background technology.
For achieving the above object, the utility model provides following technical scheme: the interactional device for fast detecting of a kind of antibody, comprise cylindrical bracket and antibody response monitoring pipe, described antibody response detector tube assigns in the groove of cylindrical bracket, described antibody response detector tube comprises antibody response detector tube body, end cap, antibody response device, aseptic drainage tube, antibody mediated immunity detects magnetic post and aseptic rubber tube interface, described end cap and antibody response detector tube body hinged, described antibody response device is fixed on the inside of antibody response detector tube body, and antibody response device and aseptic drainage tube UNICOM, described aseptic drainage tube and antibody mediated immunity detect Ci Zhu UNICOM, described antibody mediated immunity detects magnetic post and is fixed in the inside of antibody response detector tube body, described antibody mediated immunity detects magnetic post and aseptic rubber tube interface UNICOM, described aseptic rubber tube interface is connected with aseptic rubber tube, described aseptic rubber tube is connected with peristaltic pump.
Preferably, the inside of described antibody mediated immunity detection magnetic post is filled with immunomagnetic beads.
Preferably, described antibody response monitoring pipe is set to five groups, and the antibody response monitoring pipe often in group equidistantly arranges on cylindrical bracket.
Preferably, multiple-way valve is provided with between described aseptic rubber tube and peristaltic pump.
Technique effect of the present utility model and advantage: the interactional device for fast detecting of this antibody, be arranged in parallel by arranging many group antibody response monitoring pipe, interaction and the detection of different antibodies and antigen can be operated simultaneously, and antibody response device detects Ci Zhu UNICOM by aseptic drainage tube and antibody mediated immunity, and be arranged on the inside of antibody response detector tube body, the interference of external environment can not only be prevented, and also can not interfere with each other between antibody response monitoring pipe and antibody response monitoring pipe, therefore, it is possible to improve the accuracy of detection speed and detection; Can not only effluent discharge by peristaltic pump, and the interactional detection speed of antibody can be accelerated; The utility model is applicable to all biomolecule that ELISA principle detects, and directly can observe the interaction between antigen-antibody or two biomacromolecules, only need 1-2 minute, namely judges response situation by naked eyes or instrument.For the problem that background technology is mentioned, the utility model has the features such as efficient, simple and direct, quick, convenient, applicable wide.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is vertical view of the present utility model;
Fig. 3 is antibody response monitoring pipe structural representation of the present utility model.
In figure: 1 cylindrical bracket, 2 antibody response monitoring pipe, 3 antibody response detector tube bodies, 4 end caps, 5 antibody response devices, 6 aseptic drainage tubes, 7 antibody mediated immunities detect magnetic post, 8 aseptic rubber tube interfaces, 9 aseptic rubber tubes, 10 peristaltic pumps.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, be clearly and completely described the technical scheme in the utility model embodiment, obviously, described embodiment is only the utility model part embodiment, instead of whole embodiments.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of the utility model protection.
The utility model provides the interactional device for fast detecting of a kind of antibody as Figure 1-3, comprise cylindrical bracket 1 and antibody response monitoring pipe 2, described antibody response detector tube 2 assigns in the groove of cylindrical bracket 1, described antibody response monitoring pipe 2 is set to five groups, and the antibody response monitoring pipe 2 often in group equidistantly arranges on cylindrical bracket 1, described antibody response detector tube 2 comprises antibody response detector tube body 3, end cap 4, antibody response device 5, aseptic drainage tube 6, antibody mediated immunity detects magnetic post 7 and aseptic rubber tube interface 8, the inside that described antibody mediated immunity detects magnetic post 7 is filled with immunomagnetic beads, described end cap 4 is hinged with antibody response detector tube body 3, described antibody response device 5 is fixed on the inside of antibody response detector tube body 3, and antibody response device 5 and aseptic drainage tube 6 UNICOM, described aseptic drainage tube 6 detects Ci Zhu 7 UNICOM with antibody mediated immunity, described antibody mediated immunity detects magnetic post 7 and is fixed in the inside of antibody response detector tube body 3, described antibody mediated immunity detects magnetic post 7 and aseptic rubber tube interface 8 UNICOM, described aseptic rubber tube interface 8 is connected with aseptic rubber tube 9, described aseptic rubber tube 9 is connected with peristaltic pump 10, multiple-way valve is provided with between described aseptic rubber tube 9 and peristaltic pump 10.
Principle of work: the principle of this device foundation is by the specific adsorption between immunomagnetic beads and magnet, and the covalent bond principle of antigen and antibody is made.Its immunomagnetic beads surface is containing amino, carboxyl, silica-based, Streptavidin, aldehyde radical or sulfydryl isoreactivity group.By one of them biomacromolecule and immunomagnetic beads coupling.Another one biomacromolecule and enzyme, fluorophor, chemiluminescent groups or on turn luminophore coupling.If two biomacromolecules directly or indirectly interact, the magnetic compound of tool can be formed.Thus adsorbed when flowing through magnet wire.Carrying out before detection analyzes, by the immunomagnetic beads coupling of first biomacromolecule and activation, conjugate according to its character can be placed on room temperature, 4 DEG C or-20 DEG C save backup.Then close unreacted avtive spot on immunomagnetic beads with confining liquid, then magnetic bead conjugate is mixed successively with the second biomacromolecule of material to be checked, mark, add in antibody response device 5.Device is connected with peristaltic pump 10, starts peristaltic pump 10, make liquid flow.Liquid will flow through antibody mediated immunity from antibody response device 5 and detect magnetic post 7.Detected by naked eyes, fluorescence detection device, chemiluminescence detecting or upper forwarding optical detection device.Positive reaction, should detect magnetic post 7 at antibody mediated immunity and present color, and distributes along antibody mediated immunity detection magnetic post 7.
Last it is noted that the foregoing is only preferred embodiment of the present utility model; be not limited to the utility model; although be described in detail the utility model with reference to previous embodiment; for a person skilled in the art; it still can be modified to the technical scheme described in foregoing embodiments; or equivalent replacement is carried out to wherein portion of techniques feature; all within spirit of the present utility model and principle; any amendment of doing, equivalent replacement, improvement etc., all should be included within protection domain of the present utility model.
Claims (4)
1. the interactional device for fast detecting of antibody, comprise cylindrical bracket (1) and antibody response monitoring pipe (2), described antibody response detector tube (2) assigns in the groove of cylindrical bracket (1), described antibody response detector tube (2) comprises antibody response detector tube body (3), end cap (4), antibody response device (5), aseptic drainage tube (6), antibody mediated immunity detects magnetic post (7) and aseptic rubber tube interface (8), it is characterized in that: described end cap (4) is hinged with antibody response detector tube body (3), described antibody response device (5) is fixed on the inside of antibody response detector tube body (3), and antibody response device (5) and aseptic drainage tube (6) UNICOM, described aseptic drainage tube (6) and antibody mediated immunity detect magnetic post (7) UNICOM, described antibody mediated immunity detects magnetic post (7) and is fixed in the inside of antibody response detector tube body (3), described antibody mediated immunity detects magnetic post (7) and aseptic rubber tube interface (8) UNICOM, described aseptic rubber tube interface (8) is connected with aseptic rubber tube (9), described aseptic rubber tube (9) is connected with peristaltic pump (10).
2. the interactional device for fast detecting of a kind of antibody according to claim 1, is characterized in that: the inside that described antibody mediated immunity detects magnetic post (7) is filled with immunomagnetic beads.
3. the interactional device for fast detecting of a kind of antibody according to claim 1, it is characterized in that: described antibody response monitoring pipe (2) is set to five groups, and the antibody response monitoring pipe (2) often in group equidistantly arranges on cylindrical bracket (1).
4. the interactional device for fast detecting of a kind of antibody according to claim 1, is characterized in that: be provided with multiple-way valve between described aseptic rubber tube (9) and peristaltic pump (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520704046.1U CN204964518U (en) | 2015-09-11 | 2015-09-11 | Antibody interact's quick detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520704046.1U CN204964518U (en) | 2015-09-11 | 2015-09-11 | Antibody interact's quick detection device |
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| CN204964518U true CN204964518U (en) | 2016-01-13 |
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| CN201520704046.1U Expired - Fee Related CN204964518U (en) | 2015-09-11 | 2015-09-11 | Antibody interact's quick detection device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106324258A (en) * | 2016-08-29 | 2017-01-11 | 黎志春 | Auxiliary method for detecting whether genetically modified protein exists in food |
| CN106405102A (en) * | 2016-08-29 | 2017-02-15 | 黎志春 | Apparatus for aided detection of existence of transgenic protein in food |
| CN106501349A (en) * | 2017-01-05 | 2017-03-15 | 中国科学院化学研究所 | A kind of power spectral method in situ based on optics atom magnetometer |
-
2015
- 2015-09-11 CN CN201520704046.1U patent/CN204964518U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106324258A (en) * | 2016-08-29 | 2017-01-11 | 黎志春 | Auxiliary method for detecting whether genetically modified protein exists in food |
| CN106405102A (en) * | 2016-08-29 | 2017-02-15 | 黎志春 | Apparatus for aided detection of existence of transgenic protein in food |
| CN106501349A (en) * | 2017-01-05 | 2017-03-15 | 中国科学院化学研究所 | A kind of power spectral method in situ based on optics atom magnetometer |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: SHANGHAI MABSPACE BIOSCIENCES CO., LTD. Document name: Notification to Pay the Fees |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160113 Termination date: 20170911 |