CN106405102A - Apparatus for aided detection of existence of transgenic protein in food - Google Patents
Apparatus for aided detection of existence of transgenic protein in food Download PDFInfo
- Publication number
- CN106405102A CN106405102A CN201610756975.6A CN201610756975A CN106405102A CN 106405102 A CN106405102 A CN 106405102A CN 201610756975 A CN201610756975 A CN 201610756975A CN 106405102 A CN106405102 A CN 106405102A
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- plate body
- porous plate
- polypropylene membrane
- membrane tube
- transgenic protein
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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- Urology & Nephrology (AREA)
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- Food Science & Technology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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- Bioinformatics & Computational Biology (AREA)
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Abstract
The invention discloses an apparatus for aided detection of the existence of a transgenic protein in a food. The apparatus comprises: a first porous plate body; a sample adding device comprising a second porous plate body, a cone and a plurality of sample adding channels, wherein the diameter of the cone gradually decreases from bottom to top, the free end of the cone forms a sample adding hole, the plurality of sample adding channels correspond to pores in the second porous plate one to one, one ends of the plurality of sample adding channels are connected with the sample adding holes, and the other ends the plurality of sample adding channels are connected with the pores in the second porous plate; and hollow fiber polypropylene membrane tubes arranged between the first porous plate body and the second porous plate body, wherein the inner wall of every hollow fiber polypropylene membrane tube is attached with an antibody specifically binding to a transgenic protein to be detected, the upper ends of the hollow fiber polypropylene membranes tubes are fixed in the pores of the second porous plate body, the lower ends of the hollow fiber polypropylene membrane tubes are fixed in the pores of the first porous plate body, and the inner wall of every hollow fiber polypropylene membrane tube is provided with a plurality of projections and recesses.
Description
Technical field
The present invention relates to the presence or absence of the device of transgenic protein in a kind of auxiliary detection food.
Background technology
By technique for gene engineering, one or more allogenic genes are transferred in certain specific organism, and make it
Effectively give expression to corresponding product (polypeptide or protein), this process is transgenic.Given birth to genetically modified organism for Raw material processing
The food producing is exactly genetically modified food.Vegetalitas genetically modified food, animality can be divided into according to the difference in genetically modified food source
Genetically modified food and microbes genetically modified food.Genetically modified food has the advantages that more：Crop yield can be increased；Can reduce
Production cost；The ability of crop insect pest, antiviral etc. can be strengthened；Improve agricultural product storage property.The safety of genetically modified organism is commented
Valency is the premise of genetically modified organism and products thereof marketization and commercialization, and the detection of transgene component in safety evaluation is one
Important content, setting up quick, easy, the accurate detection method of genetically modified organism is that safety evaluation and management lay the foundation.For
In transgenic plant and products thereof, the specific proteinses of external source destination gene expression carry out qualitative and detection by quantitative, and its result is relatively
More accurate, reliability is also more preferable, but the means of the current detection albumen such as Elisa and Western are all comparatively laborious, go out knot
Fruit is also slower.The detection speed improving transgene component in food is current urgent problem.And it is final after gene expression
Produce is protein, and the product travelling this gene function is also protein, therefore, with the presence or absence of transgenic in detection food
Protein is one of reliable and believable result.Application number：201510005351.6 the disclosed dress of Chinese patent document
Put although can be used in the quick detection of Protein in Food, but there is result between multiple parallel laboratory tests of a sample
Differ greatly, the insecure situation of individual samples result.
Content of the invention
An object of the present invention is to provide the device with the presence or absence of transgenic protein in a kind of auxiliary detection food, should
Device can quickly and easily binding specificity ground albumen, and using simple, efficiency high.
The present invention provide technical scheme be：
With the presence or absence of the device of transgenic protein in a kind of auxiliary detection food, including：
First porous plate body, it is horizontally disposed with；
Sample adding device, it include the second porous plate body, be arranged on described second porous plate body and formed one house
The cone in space and a plurality of Loading channel, described second porous plate body is be arranged in parallel with described first porous plate body, and is located at
The lower section of described first porous plate body, the diameter of described cone is gradually reduced from down to up and its free end forms well,
Described a plurality of Loading channel corresponds setting with the hole on described second porous plate body, and one end of described a plurality of Loading channel
All connect with described well, and the other end of described a plurality of Loading channel all with described second porous plate body on a pair of hole 1
Should connect；
Doughnut polypropylene membrane tube, it is arranged between described first porous plate body and described second porous plate body, institute
Be attached with the inwall stating doughnut polypropylene membrane tube with transgenic protein to be detected specific binding antibody, described in
The upper end of hollow fiber polypropylene membrane tube is fixed in the hole of described second porous plate body, and its lower end is fixed on described first porous
In hole on plate body；
Wherein, the inwall of described doughnut polypropylene membrane tube is provided with many places projection and depression.
Preferably, described auxiliary detects that described hollow is fine with the presence or absence of in the device of transgenic protein in food
Between dimension polypropylene membrane tube and described first porous plate body, there is an angle α, wherein, 90 ° of 70 °≤α ＜.
Preferably, described auxiliary detects that described hollow is fine with the presence or absence of in the device of transgenic protein in food
Between dimension polypropylene membrane tube and described first porous plate body, there is an angle β, wherein, 90 ° of 70 °≤β ＜.
Preferably, described auxiliary detects that described hollow is fine with the presence or absence of in the device of transgenic protein in food
Polypropylene membrane tube is curvilinear is fixed between described first porous plate body and described second porous plate body for dimension.
Preferably, described auxiliary detects in the device that whether there is transgenic protein in food, more than described first
Orifice plate body and described second porous plate body are all rectangle, and the hole of described first porous plate body and described second porous plate body is all each
Self-align is point-blank.
Preferably, described auxiliary detects in the device that whether there is transgenic protein in food, more than described first
Orifice plate body and described second porous plate body are all rounded, and the hole of described first porous plate body and described second porous plate body is all respective
Along the arrangement of its circumferencial direction.
Beneficial effects of the present invention：
In the detection means that the present invention provides, the first antibody of transgenic protein is attached to doughnut polypropylene screen
On, thus whether there is transgenic egg in raw material by making the total protein of raw material to be detected flow through this detection means and detect
White matter, and by syringe, protein fluid is added in the detection means of the present invention it is easy to operate, assembly of the invention
Can effectively and rapidly detect whether there is transgene protein.Meanwhile, it is provided with a plurality of Loading channel in the well of the present invention,
Can plus a sample sample can be made with several parallel laboratory tests, the diameter of each well is all identical, and all with add
Sample hole connects, the amount of sample so flowing in every doughnut polypropylene membrane tube is the same, it is to avoid a sample
Carry out the operating error that an experiment brings.Simultaneously it is also possible to once be loaded the multiple parallel laboratory tests that can carry out a sample,
Do not need multi-pass operation, easy and simple to handle, improve work efficiency.Make testing result relatively reliable credible.
There are projection and depression in the surface of doughnut polypropylene screen, to increase its surface area, and its contact with total protein
Area, meanwhile, its being set between the first porous plate body and the second porous plate body tilts, and it is curvilinear, is having
Increased length in the space of limit so as to may associated proteins more, as such, it is desirable to the amount of sample protein matter can reduce,
Even if a small amount of gross protein also can detect.Reduce the demand to total protein quality.
Meanwhile, assembly of the invention also makes waste liquid be easy to collect, will not be to environmental pollution.
The method of the present invention is quick, accurate for the detection of transgene protein, efficiency high.
Brief description
Fig. 1 is the device in auxiliary detection food in the one of embodiment of the present invention with the presence or absence of transgenic protein
Structural representation during the hole closing of the first porous plate body；
Fig. 2 is the device in auxiliary detection food in the one of embodiment of the present invention with the presence or absence of transgenic protein
The structural representation when hole of the first porous plate body is opened.
Specific embodiment
The present invention is described in further detail below in conjunction with the accompanying drawings, to make those skilled in the art with reference to description literary composition
Word can be implemented according to this.
The present invention provides the device in a kind of auxiliary detection food with the presence or absence of transgenic protein, including：
First porous plate body 1, it is horizontally disposed with；
Sample adding device, it include the second porous plate body 2, be arranged on described second porous plate body and formed one house
The cone 3 in space and a plurality of Loading channel 4, described second porous plate body is be arranged in parallel with described first porous plate body, and position
In the lower section of described first porous plate body, the diameter of described cone is gradually reduced from down to up and its free end forms well
5, described a plurality of Loading channel corresponds setting with the hole on described second porous plate body, and the one of described a plurality of Loading channel
End all connect with described well, and the other end of described a plurality of Loading channel all with described second porous plate body on hole one by one
Corresponding connection；
Doughnut polypropylene membrane tube 6, it is arranged between described first porous plate body and described second porous plate body, institute
Be attached with the inwall stating doughnut polypropylene membrane tube with transgenic protein to be detected specific binding antibody, described in
The upper end of hollow fiber polypropylene membrane tube is fixed in the hole of described second porous plate body, and its lower end is fixed on described first porous
In hole on plate body；
Wherein, the inwall of described doughnut polypropylene membrane tube is provided with many places projection and depression.
In one of embodiment of the present invention, preferably, described doughnut polypropylene membrane tube and described first
There is between porous plate body an angle α, wherein, 90 ° of 70 °≤α ＜.
In one of embodiment of the present invention, preferably, described doughnut polypropylene membrane tube and described first
There is between porous plate body an angle β, wherein, 90 ° of 70 °≤β ＜.
In one of embodiment of the present invention, preferably, described doughnut polypropylene membrane tube is curvilinear solid
Between described first porous plate body and described second porous plate body.
In one of embodiment of the present invention, preferably, described first porous plate body and described second porous plate
Body is all rectangle, and the hole of described first porous plate body and described second porous plate body all each arranges point-blank.
In one of embodiment of the present invention, preferably, described first porous plate body and described second porous plate
Body is all rounded, and the hole of described first porous plate body and described second porous plate body all each arranges along its circumferencial direction
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in description and embodiment
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Realize other modification, therefore under the general concept being limited without departing substantially from claim and equivalency range, the present invention does not limit
In specific details with shown here as the legend with description.
Claims (6)
1. whether there is the device of transgenic protein it is characterised in that including in a kind of auxiliary detection food：
First porous plate body, it is horizontally disposed with；
Sample adding device, it includes the second porous plate body, is arranged on described second porous plate body and a formed accommodation space
Cone and a plurality of Loading channel, described second porous plate body be arranged in parallel with described first porous plate body, and is located at described
The lower section of the first porous plate body, the diameter of described cone is gradually reduced from down to up and its free end forms well, described
Hole on a plurality of Loading channel and described second porous plate body corresponds setting, and one end of described a plurality of Loading channel all with
Described well connection, and the other end of described a plurality of Loading channel is all corresponded even with the hole on described second porous plate body
Logical；
Doughnut polypropylene membrane tube, it is arranged between described first porous plate body and described second porous plate body, described in
Antibody with transgenic protein to be detected specific binding is attached with the inwall of hollow fiber polypropylene membrane tube, described hollow is fine
The upper end of dimension polypropylene membrane tube is fixed in the hole of described second porous plate body, and its lower end is fixed on described first porous plate body
On hole in；
Wherein, the inwall of described doughnut polypropylene membrane tube is provided with many places projection and depression.
2. in auxiliary detection food as claimed in claim 1 with the presence or absence of transgenic protein device it is characterised in that institute
State, between doughnut polypropylene membrane tube and described first porous plate body, there is an angle α, wherein, 90 ° of 70 °≤α ＜.
3. in auxiliary detection food as claimed in claim 1 with the presence or absence of transgenic protein device it is characterised in that institute
State, between doughnut polypropylene membrane tube and described first porous plate body, there is an angle β, wherein, 90 ° of 70 °≤β ＜.
4. in auxiliary detection food as claimed in claim 1 with the presence or absence of transgenic protein device it is characterised in that institute
State that doughnut polypropylene membrane tube is curvilinear to be fixed between described first porous plate body and described second porous plate body.
5. in auxiliary detection food as claimed in claim 1 with the presence or absence of transgenic protein device it is characterised in that institute
State the first porous plate body and described second porous plate body is all rectangle, described first porous plate body and described second porous plate body
Hole all each arrange point-blank.
6. in auxiliary detection food as claimed in claim 1 with the presence or absence of transgenic protein device it is characterised in that institute
State the first porous plate body and described second porous plate body is all rounded, described first porous plate body and described second porous plate body
Hole all each arranges along its circumferencial direction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610756975.6A CN106405102A (en) | 2016-08-29 | 2016-08-29 | Apparatus for aided detection of existence of transgenic protein in food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610756975.6A CN106405102A (en) | 2016-08-29 | 2016-08-29 | Apparatus for aided detection of existence of transgenic protein in food |
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| Publication Number | Publication Date |
|---|---|
| CN106405102A true CN106405102A (en) | 2017-02-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610756975.6A Pending CN106405102A (en) | 2016-08-29 | 2016-08-29 | Apparatus for aided detection of existence of transgenic protein in food |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106324258A (en) * | 2016-08-29 | 2017-01-11 | 黎志春 | Auxiliary method for detecting whether genetically modified protein exists in food |
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| CN103226127A (en) * | 2013-03-27 | 2013-07-31 | 清华大学 | Multi-channel micro-fluidic chip and mass spectrum combined device |
| CN103913489A (en) * | 2013-01-09 | 2014-07-09 | 北京怡成生物电子技术有限公司 | Micro biochip for real-time detection of substances in body fluids |
| CN203881776U (en) * | 2013-12-19 | 2014-10-15 | 南京卡迪奥密生物技术有限公司 | Multifunctional workstation for automatically detecting outflow of cholesterol and other analytes from cells at high throughput |
| CN104569432A (en) * | 2015-01-06 | 2015-04-29 | 通标标准技术服务有限公司 | Method and device for detecting transgenic ingredients in food |
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| CN205241660U (en) * | 2015-12-25 | 2016-05-18 | 合肥安为康医学检验有限公司 | Glutamate -pyruvate transaminase detecting device |
| CN205333651U (en) * | 2015-12-25 | 2016-06-22 | 合肥安为康医学检验有限公司 | Detection apparatus for glucose content in blood |
| CN106324258A (en) * | 2016-08-29 | 2017-01-11 | 黎志春 | Auxiliary method for detecting whether genetically modified protein exists in food |
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2016
- 2016-08-29 CN CN201610756975.6A patent/CN106405102A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103913489A (en) * | 2013-01-09 | 2014-07-09 | 北京怡成生物电子技术有限公司 | Micro biochip for real-time detection of substances in body fluids |
| CN103226127A (en) * | 2013-03-27 | 2013-07-31 | 清华大学 | Multi-channel micro-fluidic chip and mass spectrum combined device |
| CN203881776U (en) * | 2013-12-19 | 2014-10-15 | 南京卡迪奥密生物技术有限公司 | Multifunctional workstation for automatically detecting outflow of cholesterol and other analytes from cells at high throughput |
| CN104569432A (en) * | 2015-01-06 | 2015-04-29 | 通标标准技术服务有限公司 | Method and device for detecting transgenic ingredients in food |
| CN204964518U (en) * | 2015-09-11 | 2016-01-13 | 上海迈博斯生物医药科技有限公司 | Antibody interact's quick detection device |
| CN205229166U (en) * | 2015-12-25 | 2016-05-11 | 合肥安为康医学检验有限公司 | Hemoglobin detection device |
| CN205241660U (en) * | 2015-12-25 | 2016-05-18 | 合肥安为康医学检验有限公司 | Glutamate -pyruvate transaminase detecting device |
| CN205333651U (en) * | 2015-12-25 | 2016-06-22 | 合肥安为康医学检验有限公司 | Detection apparatus for glucose content in blood |
| CN106324258A (en) * | 2016-08-29 | 2017-01-11 | 黎志春 | Auxiliary method for detecting whether genetically modified protein exists in food |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106324258A (en) * | 2016-08-29 | 2017-01-11 | 黎志春 | Auxiliary method for detecting whether genetically modified protein exists in food |
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Application publication date: 20170215 |