CN104090110A - Colloidal gold immunochromatography test strip for measuring heavy metal cadmium and preparation method thereof - Google Patents
Colloidal gold immunochromatography test strip for measuring heavy metal cadmium and preparation method thereof Download PDFInfo
- Publication number
- CN104090110A CN104090110A CN201410360880.3A CN201410360880A CN104090110A CN 104090110 A CN104090110 A CN 104090110A CN 201410360880 A CN201410360880 A CN 201410360880A CN 104090110 A CN104090110 A CN 104090110A
- Authority
- CN
- China
- Prior art keywords
- heavy metal
- colloidal gold
- test paper
- metal cadmium
- paper strip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 46
- 238000012360 testing method Methods 0.000 title claims abstract description 44
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 42
- 229910052793 cadmium Inorganic materials 0.000 title claims abstract description 37
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 238000003317 immunochromatography Methods 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000000020 Nitrocellulose Substances 0.000 claims description 25
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 claims description 25
- 229920001220 nitrocellulos Polymers 0.000 claims description 25
- 239000012528 membrane Substances 0.000 claims description 16
- 239000000427 antigen Substances 0.000 claims description 14
- 102000036639 antigens Human genes 0.000 claims description 14
- 108091007433 antigens Proteins 0.000 claims description 14
- 238000011161 development Methods 0.000 claims description 11
- 229910052737 gold Inorganic materials 0.000 claims description 11
- 239000010931 gold Substances 0.000 claims description 11
- 239000000084 colloidal system Substances 0.000 claims description 8
- 241001494479 Pecora Species 0.000 claims description 6
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 238000002372 labelling Methods 0.000 claims description 4
- 239000000969 carrier Substances 0.000 claims description 3
- 239000003365 glass fiber Substances 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 17
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 238000012216 screening Methods 0.000 abstract description 5
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 229910052751 metal Inorganic materials 0.000 abstract description 2
- 239000002184 metal Substances 0.000 abstract description 2
- 229920002678 cellulose Polymers 0.000 abstract 4
- 239000001913 cellulose Substances 0.000 abstract 4
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 abstract 3
- 229910017604 nitric acid Inorganic materials 0.000 abstract 3
- 239000011521 glass Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 238000000034 method Methods 0.000 description 16
- 238000002965 ELISA Methods 0.000 description 5
- 239000012491 analyte Substances 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 239000008293 association colloid Substances 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 231100000704 bioconcentration Toxicity 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000009616 inductively coupled plasma Methods 0.000 description 1
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 150000001455 metallic ions Chemical class 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 238000012764 semi-quantitative analysis Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000005477 standard model Effects 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a colloidal gold immunochromatography test strip for measuring heavy metal cadmium and a preparation method thereof, belonging to the field of heavy metal immunochromatography. The colloidal gold immunochromatography test strip for measuring the heavy metal cadmium comprises a base plate, wherein a sample pad, a colloidal gold combination pad, a developing region and a water absorption region are arranged on the base plate in sequence from left to right; the colloidal gold combination pad is a glass cellulose film Ahlstrom8964; the developing region comprises a test line, a control line and a nitric acid cellulose film carrier; the test line, the control line and the nitric acid cellulose film carrier are Millipore135 films made of nitric acid cellulose films. According to the colloidal gold immunochromatography test strip for measuring the heavy metal cadmium, the content of the metal cadmium in the sample can be qualitatively/semiquantitatively measured; detection can be realized within 3-5 minutes and is quick; the detection sensitivity can be up to microgram/L; the operation is simple and convenient; quick screening of the trace heavy metal cadmium in the sample can be realized.
Description
Technical field
The invention belongs to heavy metal immunoassay field, more particularly, relate to colloidal gold immuno-chromatography test paper strip of a kind of mensuration heavy metal cadmium based on monoclonal antibody and preparation method thereof.
Background technology
Heavy metal contaminants is the mankind to be threatened to one of maximum pollutant, has environmental persistence, bioconcentration and three causes the features such as effect, and its content distribution in environment, food, textile and the potential hazard that may exist receive much attention always.At present, traditional heavy metal detection method adopts the instrumental methods such as atomic absorption method, inductively coupled plasma emission spectrography, inductively coupled plasma mass spectrometry mostly.Yet although these detection methods of being undertaken by instrument have certain advantage aspect degree of accuracy, sensitivity, detecting instrument is expensive, operative technique is complicated, minute is longer, it is popularized and be subject to certain restrictions.For heavy metal pollution Fast Detection Technique, the enzyme that has that research is both at home and abroad more at present suppresses method, enzyme sensor method and colourimetry etc., but said method all has the shortcoming of oneself, is difficult to realize rapid screening.Therefore, setting up a kind of simple, quick, safe detection method controls and is very important with pollution the analysis and research of heavy metal.
The heavy metal immunodetection (ELISA) that development in recent years is got up, overcome the shortcoming of said method, utilize the specific reaction of antigen-antibody to realize the detection of the pollutant can be used as antigen or antibody, the method detection sensitivity is high, high specificity, has been successfully applied to the detection of heavy metal cadmium, mercury, lead and aluminium at present.But ELISA method also has the defect of self, the detection that completes batch to be tested still at least needs 3h, can not reach the requirement of fast detecting.The same colloidal gold immuno-chromatography test paper strip method based on antigen-antibody reaction developing on immunodetection basis, the fine defect that makes up ELISA method, there is rapidly quick, sensitive and accurate, safe and simple, stable reagent and the feature such as with low cost, can in 10min, judge having or not of pollutant, also can carry out quantitative or semiquantitative mensuration, its susceptibility is compared substantially close with ELISA.
Colloidal gold immuno-chromatography test paper strip method is applied to the detection of inorganic heavy metal ion, based on heavy metal monoclonal antibody, can realizes fast qualitative examination and half-quantitative detection.But metallic ion is small-molecule substance, neither there is immunogenicity, do not there is reactionogenicity, thereby need by selecting suitable bifunctional chelating agent chelating heavy metal ion and obtaining metal monoclonal antibody specific with carrier protein couplet complete antigen synthesis immune animal yet.Therefore, the preparation of high-titer, highly specific monoclonal antibody is the bottleneck of restriction immunoassay technology.At present, this laboratory successfully makes the monoclonal antibody of heavy metal cadmium, makes colloidal gold immuno-chromatography test paper strip method become possibility for the mensuration of heavy metal cadmium.
Summary of the invention
1, the technical matters that invention will solve
Being difficult to of for prior art, colloidal gold immuno-chromatography test paper strip method being applied to exist in the detection of inorganic heavy metal ion makes the problem of the monoclonal antibody of heavy metal cadmium, the invention provides a kind of for measuring colloidal gold immuno-chromatography test paper strip of heavy metal cadmium and preparation method thereof, in order to solve the qualitative and sxemiquantitative fast detecting of heavy metal cadmium in sample, this ELISA test strip is consuming time short, sensitive, dependable performance, simple to operate.
2, technical scheme
Object of the present invention is achieved through the following technical solutions.
For measuring a colloidal gold immuno-chromatography test paper strip for heavy metal cadmium, it comprises base plate, is disposed with from left to right sample pad, collaurum pad, color development area and suction zones on described base plate;
Described collaurum pad is glass fibre element film Ahlstrom8964;
Described color development area comprises p-wire, control line and cellulose nitrate membrane carrier, and described p-wire, control line, cellulose nitrate membrane carrier are the Millipore135 film that nitrocellulose filter is made.
Preferably, the width of described immuno-chromatographic test paper strip is 4mm.
Preferably, described base plate is PVC plate.
Described for measuring a preparation method for the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, the steps include:
(A) anti-Cd-ITCBE monoclonal antibody is combined to formation with nano-colloid gold grain with the nano colloid gold of antibody labeling, and is sprayed on and on base plate, forms collaurum pad;
(B) antigens c d-ITCBE-BSA is coated on the band that nitrocellulose filter Millipore135 delimit in advance, as p-wire (being T line);
(C) be coated on the band that nitrocellulose filter Millipore135 delimit in advance, as control line (being C line) sheep anti-mouse igg two is anti-;
(D) by order from left to right by sample pad, collaurum pad, be coated with antigen p-wire, be coated with two anti-control line and thieving papers and stick on successively on described base plate, MAX line is pasted in one end at collaurum pad, is assembled into colloidal gold immuno-chromatography test paper strip;
Described thieving paper forms cellulose nitrate membrane carrier;
Collaurum pad, be coated with antigen p-wire, be coated with two anti-control lines and cellulose nitrate membrane carriers form color development area, on this base plate, one end adjacent with cellulose nitrate membrane carrier is suction zones.
Preferably, on described immuno-chromatographic test paper strip, the particle diameter of described nano-colloid gold grain is 10-20nm.
Preferably, described anti-Cd-ITCBE monoclonal antibody, the coated concentration of antibody is 5 X 10
4times dilute concentration.
Preferably, described immuno-chromatographic test paper strip coating antigen Cd-ITCBE-BSA, coated concentration is 5 X 10
3times dilute concentration, sheep anti-mouse igg two anti-coated concentration are 5 X 10
3times dilute concentration.
3, beneficial effect
Than prior art, beneficial effect of the present invention is:
(1) the present invention is based on Cd monoclonal antibody for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, pass through competition law, qualitative/semiquantitative determination Gold Samples belongs to the content of cadmium, can in 3-5 minute, realize detection, detect faster, detection sensitivity can reach μ g/L, easy and simple to handle, sensitive reliable, can realize the rapid screening of trace heavy metal cadmium in sample;
(2) the present invention adopts the colloidal gold immuno-chromatography test paper strip of the monoclonal antibody based on anti-Cd-ITCBE to carry out fast qualitative, sxemiquantitative test to heavy metal cadmium in sample, can be widely used in analytical test and rapid screening containing the aqueous sample of heavy metal cadmium;
(3), in prior art, the preparation of high-titer, highly specific monoclonal antibody is the bottleneck of restriction immunoassay technology.At present, the present invention successfully makes the monoclonal antibody of heavy metal cadmium, makes colloidal gold immuno-chromatography test paper strip method become possibility for the mensuration of heavy metal cadmium.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
In figure: 1, sample pad; 2, collaurum pad; 3, p-wire; 4, control line; 5, color development area; 6, cellulose nitrate membrane carrier; 7, suction zones.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in detail.
Embodiment 1
As shown in Figure 1, measure the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, its main body is base plate, is disposed with from left to right sample pad 1, collaurum pad 2, color development area 5 and suction zones 7 on base plate.Color development area 5 comprises p-wire 3, control line 4 and cellulose nitrate membrane carrier 6, the Millipore135 film that described p-wire 3, control line 4, cellulose nitrate membrane carrier 6 are made for nitrocellulose filter.The width of immuno-chromatographic test paper strip is 4mm.Base plate is PVC plate.
The preparation method of this test paper, step is as follows:
(A) will resist Cd-ITCBE monoclonal antibody and nano-colloid gold grain (mean grain size 10-20nm) in conjunction with the nano colloid gold forming with antibody labeling, and be sprayed on and on base plate, form collaurum pad 2; Wherein, anti-Cd-ITCBE monoclonal antibody, the coated concentration of antibody is 5 X 10
4times dilute concentration.
(B) antigens c d-ITCBE-BSA is coated on the band that nitrocellulose filter Millipore135 delimit in advance, as p-wire 3; Wherein, described immuno-chromatographic test paper strip coating antigen Cd-ITCBE-BSA, coated concentration is 5 X 10
3times dilute concentration.
(C) be coated on the band that nitrocellulose filter Millipore135 delimit in advance, as control line 4 sheep anti-mouse igg two is anti-; Wherein, sheep anti-mouse igg two anti-coated concentration are 5 X 10
3times dilute concentration.
(D) by order from left to right by sample pad 1, collaurum pad 2, be coated with antigen p-wire 3, be coated with two anti-control line 4 and cellulose nitrate membrane carriers 6 and stick on successively on described base plate, MAX line is pasted in one end at collaurum pad 2, is assembled into colloidal gold immuno-chromatography test paper strip;
Cellulose nitrate membrane carrier 6 is.On this base plate, one end adjacent with cellulose nitrate membrane carrier 6 is suction zones 7.
Immuno-chromatographic test paper strip based on above-mentioned system, in use, first, the standard solution of the Cd that contains variable concentrations and excessive EDTA chelating are formed to the standard model containing cadmium, drip in sample pad 1, by the capillarity of tunica fibrosa, sample flows through the collaurum pad 2 of self-control Cd-ITCBE labeling of monoclonal antibody.For p-wire 3 (T line), control line 4 (C line), by observing the variation of test strips color in color development area 5, qualitatively judge the content whether sample contains heavy metal cadmium in heavy metal cadmium and semi-quantitative analysis sample.
Sample is competed the monoclonal antibody-collaurum bond on association colloid gold pad 2 with the Cd-ITCBE-BSA coating antigen being coated on T line, Cd content proportion relation in the depth of T line color and sample, thereby sensing range and the sensitivity of definite colloidal gold immuno-chromatography test paper strip Fast Measurement heavy metal Cd; If analyte concentration is very high in sample, the middle Cd-EDTA of sample completely on collaurum pad 2 monoclonal antibody-colloidal gold composite be combined, so T line does not develop the color; If analyte is lower in sample, T line color is compared shallow with C line color; If, without analyte, T line color is identical with the C line color depth in sample; Thus, can set up the method for sxemiquantitative/qualitative Fast Measurement heavy metal Cd.
The present embodiment based on Cd monoclonal antibody for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, pass through competition law, qualitative/semiquantitative determination Gold Samples belongs to the content of cadmium, can in 3-5 minute, realize detection, detect quicker, detection sensitivity can reach μ g/L, can realize the rapid screening of trace heavy metal cadmium in sample.
Claims (7)
1. one kind for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, it is characterized in that, it comprises base plate, is disposed with from left to right sample pad (1), collaurum pad (2), color development area (5) and suction zones (7) on described base plate;
Described collaurum pad (2) is glass fibre element film Ahlstrom8964;
Described color development area (5) comprises p-wire (3), control line (4) and cellulose nitrate membrane carrier (6), the Millipore135 film that described p-wire (3), control line (4), cellulose nitrate membrane carrier (6) are made for nitrocellulose filter.
2. according to claim 1 a kind ofly it is characterized in that for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, the width of described immuno-chromatographic test paper strip is 4mm.
3. according to claim 2 a kind ofly it is characterized in that for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, described base plate is PVC plate.
4. claimed in claim 1 for measuring a preparation method for the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, the steps include:
(A) anti-Cd-ITCBE monoclonal antibody is combined with nano-colloid gold grain and forms the nano colloid gold of antibody labeling, and be sprayed on and on base plate, form collaurum pad (2);
(B) antigens c d-ITCBE-BSA is coated on the band that nitrocellulose filter Millipore135 delimit in advance, as p-wire (3);
(C) be coated on the band that nitrocellulose filter Millipore135 delimit in advance, as control line (4) sheep anti-mouse igg two is anti-;
(D) by order from left to right by sample pad (1), collaurum pad (2), be coated with antigen p-wire (3), be coated with two anti-control line (4) and thieving papers and stick on successively on described base plate, MAX line is pasted in one end at collaurum pad (2), is assembled into colloidal gold immuno-chromatography test paper strip;
Described thieving paper forms cellulose nitrate membrane carrier (6);
Collaurum pad (2), be coated with antigen p-wire (3), be coated with two anti-control lines (4) and cellulose nitrate membrane carriers (6) form color development area (5), on this base plate, one end adjacent with cellulose nitrate membrane carrier (6) is suction zones (7).
5. according to claim 4ly for measuring the preparation method of the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, it is characterized in that, on described immuno-chromatographic test paper strip, the particle diameter of described nano-colloid gold grain is 10-20nm.
According to described in claim 4 or 5 for measuring the preparation method of the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, it is characterized in that, described anti-Cd-ITCBE monoclonal antibody, the coated concentration of antibody is 5 X 10
4times dilute concentration.
7. according to claim 4ly for measuring the preparation method of the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, it is characterized in that, described immuno-chromatographic test paper strip coating antigen Cd-ITCBE-BSA, coated concentration is 5 X 10
3times dilute concentration, sheep anti-mouse igg two anti-coated concentration are 5 X 10
3times dilute concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410360880.3A CN104090110A (en) | 2014-07-25 | 2014-07-25 | Colloidal gold immunochromatography test strip for measuring heavy metal cadmium and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410360880.3A CN104090110A (en) | 2014-07-25 | 2014-07-25 | Colloidal gold immunochromatography test strip for measuring heavy metal cadmium and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104090110A true CN104090110A (en) | 2014-10-08 |
Family
ID=51637842
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410360880.3A Pending CN104090110A (en) | 2014-07-25 | 2014-07-25 | Colloidal gold immunochromatography test strip for measuring heavy metal cadmium and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104090110A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110632294A (en) * | 2019-09-26 | 2019-12-31 | 北京丹大生物技术有限公司 | Kit for rapidly detecting cadmium content in sample |
| CN111220806A (en) * | 2020-03-09 | 2020-06-02 | 上海雄图生物科技有限公司 | Method for manufacturing ultrasensitive rapid time-resolved fluorescence immunochromatographic test strip |
| CN113030466A (en) * | 2021-03-04 | 2021-06-25 | 浙江省农业科学院 | Colloidal gold immunochromatographic test strip for detecting heavy metal cadmium in soil and preparation method thereof |
| CN113607951A (en) * | 2021-07-29 | 2021-11-05 | 上海师范大学 | Immunomacrosphere chromatography test strip for rapidly detecting heavy metal cadmium and preparation method thereof |
| CN113791209B (en) * | 2021-08-12 | 2024-01-26 | 重庆工商大学 | Immunochromatography test strip without hook effect and preparation method thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101016337A (en) * | 2007-03-02 | 2007-08-15 | 南京农业大学 | Preparing method of heavy metal cadmium monoclonal antibody |
| CN101139396A (en) * | 2007-08-08 | 2008-03-12 | 华南理工大学 | Cadmium chelate complex monoclonal antibody and preparation method and usage thereof |
| CN101240023A (en) * | 2008-03-14 | 2008-08-13 | 南京大学 | Preparation of heavy metal cadmium polyclonal antibody and method for measuring enzyme linked immunity absorption |
| CN101451998A (en) * | 2008-12-30 | 2009-06-10 | 暨南大学 | Cadmium ion colloidal gold immune chromatography rapid detecting test paper strip, preparation method and application |
| CN101576563A (en) * | 2009-06-19 | 2009-11-11 | 暨南大学 | Test paper for rapidly detecting immunochromatography of cadmium ion colloidal gold and preparation method and application thereof |
| CN201773104U (en) * | 2010-09-07 | 2011-03-23 | 北京欧凯纳斯科技有限公司 | Detecting card for detecting various heavy metals |
| US20130309656A1 (en) * | 2012-05-17 | 2013-11-21 | David C. Davis | Antibody detection method and device for a saliva sample from a non-human animal |
| CN203981687U (en) * | 2014-07-25 | 2014-12-03 | 江苏省检验检疫科学技术研究院 | A kind of for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium |
-
2014
- 2014-07-25 CN CN201410360880.3A patent/CN104090110A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101016337A (en) * | 2007-03-02 | 2007-08-15 | 南京农业大学 | Preparing method of heavy metal cadmium monoclonal antibody |
| CN101139396A (en) * | 2007-08-08 | 2008-03-12 | 华南理工大学 | Cadmium chelate complex monoclonal antibody and preparation method and usage thereof |
| CN101240023A (en) * | 2008-03-14 | 2008-08-13 | 南京大学 | Preparation of heavy metal cadmium polyclonal antibody and method for measuring enzyme linked immunity absorption |
| CN101451998A (en) * | 2008-12-30 | 2009-06-10 | 暨南大学 | Cadmium ion colloidal gold immune chromatography rapid detecting test paper strip, preparation method and application |
| CN101576563A (en) * | 2009-06-19 | 2009-11-11 | 暨南大学 | Test paper for rapidly detecting immunochromatography of cadmium ion colloidal gold and preparation method and application thereof |
| CN201773104U (en) * | 2010-09-07 | 2011-03-23 | 北京欧凯纳斯科技有限公司 | Detecting card for detecting various heavy metals |
| US20130309656A1 (en) * | 2012-05-17 | 2013-11-21 | David C. Davis | Antibody detection method and device for a saliva sample from a non-human animal |
| CN203981687U (en) * | 2014-07-25 | 2014-12-03 | 江苏省检验检疫科学技术研究院 | A kind of for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium |
Non-Patent Citations (3)
| Title |
|---|
| 向军俭，等: "胶体金免疫层析法快速检测水样品中的镉离子", 《中国生物制品学杂志》 * |
| 吴凡: "重金属镉胶体金免疫层析快速测定方法及凹凸棒土对其吸附去除研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 王津: "重金属镉免疫检测方法研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110632294A (en) * | 2019-09-26 | 2019-12-31 | 北京丹大生物技术有限公司 | Kit for rapidly detecting cadmium content in sample |
| CN111220806A (en) * | 2020-03-09 | 2020-06-02 | 上海雄图生物科技有限公司 | Method for manufacturing ultrasensitive rapid time-resolved fluorescence immunochromatographic test strip |
| CN113030466A (en) * | 2021-03-04 | 2021-06-25 | 浙江省农业科学院 | Colloidal gold immunochromatographic test strip for detecting heavy metal cadmium in soil and preparation method thereof |
| CN113607951A (en) * | 2021-07-29 | 2021-11-05 | 上海师范大学 | Immunomacrosphere chromatography test strip for rapidly detecting heavy metal cadmium and preparation method thereof |
| CN113791209B (en) * | 2021-08-12 | 2024-01-26 | 重庆工商大学 | Immunochromatography test strip without hook effect and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106872420B (en) | Kit and method for time-resolved fluorescence quantitative detection of microalbuminuria | |
| Fang et al. | Barcode lateral flow immunochromatographic strip for prostate acid phosphatase determination | |
| JP5132664B2 (en) | Immunochromatographic method | |
| Li et al. | Development of a 3D origami multiplex electrochemical immunodevice using a nanoporous silver-paper electrode and metal ion functionalized nanoporous gold–chitosan | |
| CN104090110A (en) | Colloidal gold immunochromatography test strip for measuring heavy metal cadmium and preparation method thereof | |
| Lin et al. | Washing-free centrifugal microchip fluorescence immunoassay for rapid and point-of-care detection of protein | |
| WO2021065300A1 (en) | Immunological test method and jig for condensation | |
| JP2008522169A (en) | Lateral flow test device provides improved test result effectiveness | |
| EP2558204B1 (en) | Assay apparatus incorporating a microfluidic channel and assay method | |
| CN111033237B (en) | Sandwich assay for measuring analytes, including high concentrations of analytes, using decreasing signal portions of dose-response curves | |
| CN110927395A (en) | Triple test strip for detecting methamphetamine, morphine and ketamine as well as preparation method and application method thereof | |
| CN103954753A (en) | Quantitative determination method of immune chromatography test strip | |
| CN111684280A (en) | Lateral flow assay and method for detecting high concentrations of analytes | |
| JP2006119044A5 (en) | ||
| CN113567668A (en) | Preparation and application of fluorescent immunochromatographic test strip for exosome quantification | |
| Zhang et al. | SERS based immunochromatographic assay for rapid and quantitative determination of bisphenol A | |
| CN203981687U (en) | A kind of for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium | |
| CN117031022A (en) | Kit and method for detecting plasmin by fluorescence immunochromatography | |
| CN102819691B (en) | Modeling method for biochemical reaction process of immunochromatography test strip | |
| CN113113079B (en) | Method for identifying hook effect in quantitative immunochromatography test | |
| CN204287198U (en) | Omnidistance C reactive protein immunochromatographiassay assay quantitative detection test paper | |
| US20140011190A1 (en) | Method for performing a rapid test | |
| Lee et al. | Pump-free glass-based capillary microfluidic immuno-assay chip for electrochemical detection of prostate-specific antigen | |
| CN110927381A (en) | Test strip for detecting 6-monoacetylmorphine and preparation method and application method thereof | |
| CN110927396A (en) | Duplex test strip for detecting methamphetamine and morphine as well as preparation method and application method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20141008 |