CN203981687U - A kind of for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium - Google Patents
A kind of for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium Download PDFInfo
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- CN203981687U CN203981687U CN201420417515.7U CN201420417515U CN203981687U CN 203981687 U CN203981687 U CN 203981687U CN 201420417515 U CN201420417515 U CN 201420417515U CN 203981687 U CN203981687 U CN 203981687U
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- Prior art keywords
- heavy metal
- test paper
- paper strip
- colloidal gold
- metal cadmium
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- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 42
- 238000012360 testing method Methods 0.000 title claims abstract description 35
- 229910052793 cadmium Inorganic materials 0.000 title claims abstract description 33
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 title claims abstract description 33
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 238000003317 immunochromatography Methods 0.000 title claims abstract description 24
- 239000000020 Nitrocellulose Substances 0.000 claims abstract description 23
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 claims abstract description 23
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 23
- 239000012528 membrane Substances 0.000 claims abstract description 15
- 238000011161 development Methods 0.000 claims abstract description 12
- 239000003365 glass fiber Substances 0.000 claims abstract description 3
- 238000001514 detection method Methods 0.000 abstract description 17
- 229910052737 gold Inorganic materials 0.000 abstract description 9
- 239000010931 gold Substances 0.000 abstract description 9
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 238000012216 screening Methods 0.000 abstract description 5
- 238000003018 immunoassay Methods 0.000 abstract description 4
- 238000000034 method Methods 0.000 description 16
- 239000000427 antigen Substances 0.000 description 10
- 102000036639 antigens Human genes 0.000 description 10
- 108091007433 antigens Proteins 0.000 description 10
- 238000002965 ELISA Methods 0.000 description 5
- 239000000084 colloidal system Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 4
- 239000012491 analyte Substances 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 239000008293 association colloid Substances 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 231100000704 bioconcentration Toxicity 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000009616 inductively coupled plasma Methods 0.000 description 1
- 238000001095 inductively coupled plasma mass spectrometry Methods 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000001455 metallic ions Chemical class 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 238000012764 semi-quantitative analysis Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000005477 standard model Effects 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
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- Peptides Or Proteins (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model discloses a kind ofly for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, belong to heavy metal immunoassay field.For measuring a colloidal gold immuno-chromatography test paper strip for heavy metal cadmium, it comprises base plate, is disposed with from left to right sample pad, collaurum pad, color development area and suction zones on base plate; Collaurum pad is glass fibre element film Ahlstrom8964; Color development area comprises p-wire, control line and cellulose nitrate membrane carrier, and p-wire, control line, cellulose nitrate membrane carrier are the Millipore135 film that nitrocellulose filter is made.The utility model based on Cd monoclonal antibody for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, pass through competition law, qualitative/semiquantitative determination Gold Samples belongs to the content of cadmium, can in 3-5 minute, realize detection, detect quicker, detection sensitivity can reach μ g/L, easy and simple to handle, can realize the rapid screening of trace heavy metal cadmium in sample.
Description
Technical field
The invention belongs to heavy metal immunoassay field, more particularly, relate to a kind of colloidal gold immuno-chromatography test paper strip of the mensuration heavy metal cadmium based on monoclonal antibody.
Background technology
Heavy metal contaminants is the mankind to be threatened to one of maximum pollutant, has environmental persistence, bioconcentration and three causes the features such as effect, and its content distribution in environment, food, textile and the potential hazard that may exist receive much attention always.At present, traditional heavy metal detection method adopts the instrumental methods such as atomic absorption method, inductively coupled plasma emission spectrography, inductively coupled plasma mass spectrometry mostly.But although these detection methods of being undertaken by instrument have certain advantage aspect degree of accuracy, sensitivity, detecting instrument costliness, operative technique complexity, minute are longer, it is popularized and be subject to certain restrictions.For heavy metal pollution Fast Detection Technique, the enzyme that has that research is both at home and abroad more at present suppresses method, enzyme sensor method and colourimetry etc., but said method all has the shortcoming of oneself, is difficult to realize rapid screening.Therefore, setting up a kind of simple, quick, safe detection method controls and is very important with pollution the analysis and research of heavy metal.
The heavy metal immunodetection (ELISA) that development in recent years is got up, overcome the shortcoming of said method, utilize the specific reaction of antigen-antibody to realize the detection of the pollutant that can be used as antigen or antibody, the method detection sensitivity is high, high specificity, has been successfully applied to the detection of heavy metal cadmium, mercury, lead and aluminium at present.But ELISA method also has the defect of self, the detection that completes batch to be tested still at least needs 3h, can not reach the requirement of fast detecting.The same colloidal gold immuno-chromatography test paper strip method based on antigen-antibody reaction developing on immunodetection basis, the fine defect that makes up ELISA method, there is rapidly quick, sensitive and accurate, safe and simple, stable reagent and the feature such as with low cost, can in 10min, judge having or not of pollutant, also can carry out quantitative or semiquantitative mensuration, its susceptibility is substantially close compared with ELISA.
Colloidal gold immuno-chromatography test paper strip method is applied to the detection of inorganic heavy metal ion, based on heavy metal monoclonal antibody, can realizes fast qualitative examination and half-quantitative detection.But metallic ion is small-molecule substance, neither there is immunogenicity, do not there is reactionogenicity, thereby need by selecting suitable bifunctional chelating agent chelating heavy metal ion and obtaining metal monoclonal antibody specific with carrier protein couplet complete antigen synthesis immune animal yet.Therefore, the preparation of high-titer, highly specific monoclonal antibody is the bottleneck of restriction immunoassay technology.At present, this laboratory successfully makes the monoclonal antibody of heavy metal cadmium, makes colloidal gold immuno-chromatography test paper strip method become possibility for the mensuration of heavy metal cadmium.
Summary of the invention
1, the technical matters that invention will solve
For prior art, colloidal gold immuno-chromatography test paper strip method is applied to the problem that is difficult to the monoclonal antibody that makes heavy metal cadmium existing in the detection of inorganic heavy metal ion, the invention provides a kind of for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, in order to solve the qualitative and sxemiquantitative fast detecting of heavy metal cadmium in sample, this ELISA test strip is consuming time short, sensitive, dependable performance, simple to operate.
2, technical scheme
Object of the present invention is achieved through the following technical solutions.
For measuring a colloidal gold immuno-chromatography test paper strip for heavy metal cadmium, it comprises base plate, is disposed with from left to right sample pad, collaurum pad, color development area and suction zones on described base plate;
Described collaurum pad is glass fibre element film Ahlstrom8964;
Described color development area comprises p-wire, control line and cellulose nitrate membrane carrier, and described p-wire, control line, cellulose nitrate membrane carrier is the Millipore135 film that nitrocellulose filter is made.
Preferably, the width of described immuno-chromatographic test paper strip is 4mm.
Preferably, described base plate is PVC plate.
Described for measuring the preparation method of colloidal gold immuno-chromatography test paper strip for heavy metal cadmium, the steps include:
(A) anti-Cd-ITCBE monoclonal antibody is combined to the nano colloid gold of formation with antibody labeling with nano-colloid gold grain, and is sprayed on and on base plate, forms collaurum pad;
(B) antigens c d-ITCBE-BSA is coated on the band that nitrocellulose filter Millipore135 delimit in advance, as p-wire (being T line);
(C) be coated on the band that nitrocellulose filter Millipore135 delimit in advance, as control line (being C line) anti-sheep anti-mouse igg two;
(D) by order from left to right by sample pad, collaurum pad, be coated with antigen p-wire, be coated with two anti-control line and thieving papers and stick on successively on described base plate, MAX line is pasted in one end at collaurum pad, is assembled into colloidal gold immuno-chromatography test paper strip;
Described thieving paper forms cellulose nitrate membrane carrier;
Collaurum pad, be coated with antigen p-wire, be coated with two anti-control lines and cellulose nitrate membrane carriers form color development area, on this base plate, one end adjacent with cellulose nitrate membrane carrier is suction zones.
Preferably, on described immuno-chromatographic test paper strip, the particle diameter of described nano-colloid gold grain is 10-20nm.
Preferably, described anti-Cd-ITCBE monoclonal antibody, the coated concentration of antibody is 5 X 10
4times dilute concentration.
Preferably, described immuno-chromatographic test paper strip coating antigen Cd-ITCBE-BSA, coated concentration is 5 X 10
3times dilute concentration, sheep anti-mouse igg two anti-coated concentration are 5 X 10
3times dilute concentration.
3, beneficial effect
Than prior art, beneficial effect of the present invention is:
(1) the present invention is based on Cd monoclonal antibody for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, pass through competition law, qualitative/semiquantitative determination Gold Samples belongs to the content of cadmium, can in 3-5 minute, realize detection, detect faster, detection sensitivity can reach μ g/L, easy and simple to handle, sensitive reliable, can realize the rapid screening of trace heavy metal cadmium in sample;
(2) the present invention adopts the colloidal gold immuno-chromatography test paper strip of the monoclonal antibody based on anti-Cd-ITCBE to carry out fast qualitative, sxemiquantitative test to heavy metal cadmium in sample, can be widely used in analytical test and rapid screening containing the aqueous sample of heavy metal cadmium;
(3), in prior art, the preparation of high-titer, highly specific monoclonal antibody is the bottleneck of restriction immunoassay technology.At present, the present invention successfully makes the monoclonal antibody of heavy metal cadmium, makes colloidal gold immuno-chromatography test paper strip method become possibility for the mensuration of heavy metal cadmium.
Brief description of the drawings
Fig. 1 is structural representation of the present invention.
In figure: 1, sample pad; 2, collaurum pad; 3, p-wire; 4, control line; 5, color development area; 6, cellulose nitrate membrane carrier; 7, suction zones.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in detail.
Embodiment 1
As shown in Figure 1, measure the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, its main body is base plate, is disposed with from left to right sample pad 1, collaurum pad 2, color development area 5 and suction zones 7 on base plate.Color development area 5 comprises p-wire 3, control line 4 and cellulose nitrate membrane carrier 6, the Millipore135 film that described p-wire 3, control line 4, cellulose nitrate membrane carrier 6 made for nitrocellulose filter.The width of immuno-chromatographic test paper strip is 4mm.Base plate is PVC plate.
The preparation method of this test paper, step is as follows:
(A) will resist Cd-ITCBE monoclonal antibody and nano-colloid gold grain (mean grain size 10-20nm) in conjunction with the nano colloid gold forming with antibody labeling, and be sprayed on and on base plate, form collaurum pad 2; Wherein, anti-Cd-ITCBE monoclonal antibody, the coated concentration of antibody is 5 X 10
4times dilute concentration.
(B) antigens c d-ITCBE-BSA is coated on the band that nitrocellulose filter Millipore135 delimit in advance, as p-wire 3; Wherein, described immuno-chromatographic test paper strip coating antigen Cd-ITCBE-BSA, coated concentration is 5 X 10
3times dilute concentration.
(C) be coated on the band that nitrocellulose filter Millipore135 delimit in advance, as control line 4 anti-sheep anti-mouse igg two; Wherein, sheep anti-mouse igg two anti-coated concentration are 5 X 10
3times dilute concentration.
(D) by order from left to right by sample pad 1, collaurum pad 2, be coated with antigen p-wire 3, be coated with two anti-control line 4 and cellulose nitrate membrane carriers 6 and stick on successively on described base plate, MAX line is pasted in one end at collaurum pad 2, is assembled into colloidal gold immuno-chromatography test paper strip;
Cellulose nitrate membrane carrier 6 is.On this base plate, one end adjacent with cellulose nitrate membrane carrier 6 is suction zones 7.
Based on the immuno-chromatographic test paper strip of above-mentioned system, in use, first, the standard solution of the Cd that contains variable concentrations and excessive EDTA chelating are formed to the standard model containing cadmium, drip in sample pad 1, by the capillarity of tunica fibrosa, sample flows through the collaurum pad 2 of self-control Cd-ITCBE labeling of monoclonal antibody.For p-wire 3 (T line), control line 4 (C line), by observe the variation of test strips color in color development area 5, qualitatively judge the content whether sample contains heavy metal cadmium in heavy metal cadmium and semi-quantitative analysis sample.
Sample is competed the monoclonal antibody-collaurum bond on association colloid gold pad 2 with the Cd-ITCBE-BSA coating antigen being coated on T line, Cd content proportion relation in the depth of T line color and sample, thereby sensing range and the sensitivity of definite colloidal gold immuno-chromatography test paper strip Fast Measurement heavy metal Cd; If analyte concentration is very high in sample, the middle Cd-EDTA of sample completely on collaurum pad 2 monoclonal antibody-colloidal gold composite be combined, therefore T line does not develop the color; If analyte is lower in sample, T line color is compared shallow with C line color; If, without analyte, T line color is identical with the C line color depth in sample; Thus, can set up the method for sxemiquantitative/qualitative Fast Measurement heavy metal Cd.
The present embodiment based on Cd monoclonal antibody for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, pass through competition law, qualitative/semiquantitative determination Gold Samples belongs to the content of cadmium, can in 3-5 minute, realize detection, detect quicker, detection sensitivity can reach μ g/L, can realize the rapid screening of trace heavy metal cadmium in sample.
Claims (3)
1. one kind for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, it is characterized in that, it comprises base plate, is disposed with from left to right sample pad (1), collaurum pad (2), color development area (5) and suction zones (7) on described base plate;
Described collaurum pad (2) is glass fibre element film Ahlstrom8964;
Described color development area (5) comprises p-wire (3), control line (4) and cellulose nitrate membrane carrier (6), the Millipore135 film that described p-wire (3), control line (4), cellulose nitrate membrane carrier (6) made for nitrocellulose filter.
2. according to claim 1 a kind ofly it is characterized in that for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, the width of described immuno-chromatographic test paper strip is 4mm.
3. according to claim 2 a kind ofly it is characterized in that for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium, described base plate is PVC plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420417515.7U CN203981687U (en) | 2014-07-25 | 2014-07-25 | A kind of for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420417515.7U CN203981687U (en) | 2014-07-25 | 2014-07-25 | A kind of for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium |
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| Publication Number | Publication Date |
|---|---|
| CN203981687U true CN203981687U (en) | 2014-12-03 |
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| CN201420417515.7U Expired - Fee Related CN203981687U (en) | 2014-07-25 | 2014-07-25 | A kind of for measuring the colloidal gold immuno-chromatography test paper strip of heavy metal cadmium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104090110A (en) * | 2014-07-25 | 2014-10-08 | 江苏省检验检疫科学技术研究院 | Colloidal gold immunochromatography test strip for measuring heavy metal cadmium and preparation method thereof |
-
2014
- 2014-07-25 CN CN201420417515.7U patent/CN203981687U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104090110A (en) * | 2014-07-25 | 2014-10-08 | 江苏省检验检疫科学技术研究院 | Colloidal gold immunochromatography test strip for measuring heavy metal cadmium and preparation method thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141203 Termination date: 20150725 |
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| EXPY | Termination of patent right or utility model |