CN101625359A - Syphilis-diagnosis special multichannel micro-fluidic chip based on electrode modification technology - Google Patents
Syphilis-diagnosis special multichannel micro-fluidic chip based on electrode modification technology Download PDFInfo
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Abstract
The invention relates to a syphilis-diagnosis special multichannel micro-fluidic chip based on an electrode modification technology, belonging to the field of analytical testing. The quick and cheap diagnosis of syphilitic patients is one of the improved objectives of a relative diagnosis and treatment technology. The invention provides a micro-fluidic chip which points to the objective. The scheme is characterized in that pipelines with parallel structure are arranged in the chip, the parallel structure comprises four branched pipelines which are connected in parallel, four magnetic bead string working electrodes are respectively assembled in the four branched pipelines, each magnetic bead string working electrode comprises a magnetic bead string electrode and a gold size sensitive film, the gold size sensitive film is attached to the magnetic bead string electrode and is embedded with syphilitic specific antigens, and the gold size sensitive films arranged on the surfaces of the four magnetic bead string working electrodes are respectively embedded with four different syphilitic specific antigen materials of syphilitic specific antigens TP0684, TP 0453, TP 0821 and TP 0391. The micro-fluidic chip is beneficial to improving the syphilitic diagnostic efficiency.
Description
Technical field
The present invention relates to a kind of syphilis-diagnosis special multichannel micro-fluidic chip, belong to the analytical test field based on electrode modification technology.
Background technology
Syphilis (Syphilis) be by microspironema pallidum (T.pallidum, a kind of sexually transmitted disease that TP) causes (Sexually transmitted disease, STD).Microspironema pallidum only infects the mankind under natural situation, and the people is the unique infection sources of syphilis, main trafficability characteristic contact transmission.Also can pass through the placenta vertical transmission in addition, cause fetal congenital syphilis.A few peoples also can infect through blood transfusion.Since the eighties, syphilis is revivable once more in China, according to national venereal disease control center statistics, China's syphilis incidence of disease annual growth reaches 52.7%, and the congenital syphilis and the neurolues incidence of disease increase obviously, the trend that rises is gradually also arranged in the whole world, syphilis not only become international serious society and public health problem, and be one of principal disease of harm population health.Therefore, select simple, fast, hypersensitivity and specificity diagnostic method and reagent, to the syphilis early diagnosis, find the infection sources, control syphilis is propagated significant.
Controller used in syphilis diagnosis method commonly used at present has etiological examination, non-syphilitic leptospira antigen serum test, the test of microspironema pallidum hemagglutination (TPHA), polymerase chain reaction (PCR) and TP quick detection test paper bar etc.The principle of etiological examination is to get the exudate of patient's infringement or the tissue fluid that lymphnode puncture obtains, observe spirochetal characteristic morphologic and mode of motion, in theory early syphilis there is higher recall rate, but the restriction (comprising prescription on individual diagnosis medication, high-quality fluorescent microscope, reagent and technology etc.) that is subjected to condition is also more, and therefore actual recall rate is not high.The principle of non-syphilitic leptospira antigen serum test is the serology primary dcreening operation test commonly used of current diagnosis syphilis, though in a lot of documents, all mention non-syphilitic leptospira antigen serum test simple and easy, quick, be easy to observations, the screening serum that is suitable for a large amount of crowds, but susceptibility, specificity are relatively poor, biologic fale-positive and false negative reaction appear sometimes, cause omission and flase drop easily, so be not suitable for the diagnosis of primary syphilis, congenital syphilis, neurolues.Though its susceptibility of TPHA and specificity height mainly are used to the syphilis confirmed diagnosis test, and observation of curative effect is had little significance.Though TP quick detection test paper bar has overcome defectives such as length detection time, reagent costliness, domestic temporarily do not have its sensitivity of play-by-play and specificity effect, so be not useable for confirmed diagnosis test.The PCR method is newer diagnostic method, and it can carry out early stage antidiastole to genital ulcer, distinguishes syphilis, genital herpes and chancroid.But the PCR method of current diagnosis syphilis still exist primer non-specific, to shortcomings such as susceptibility such as serum/whole blood are low.And when the skin damage begins healing, secretion minimizing, serological test positive reaction, adopt PCR method just improper.In addition, PCR method is subject to materials such as tissue in the sample and cell fragment and suppresses, and causes the PCR false negative result, so detect should not be as declaring more for PCR.
Detection methods such as non-syphilitic leptospira antigen serum test, TPHA and PCR and TP quick detection test paper bar all belong to immunoassay.Above immunoassay itself has very strong selectivity, to the early diagnosis of infectious disease, judges that all there is important meaning aspects such as curative effect and prognosis.Yet the detection of serious infectious diseases is very complicated, and traditional immunoassay only detects at monospecific antibody usually.Because a kind of diagnosis antibody can appear at different pathogenic bacteria, or different diagnosis antibody can occur simultaneously in same pathogenic bacteria, therefore, carry out multiple efficiently immune analysis method (SMIAs), the kind and the quantity of, quantitative response various pathogenic bacteria antibody qualitative truly from the angle of biological correlativity, in early days prevention and the multiple infectious disease of joint inspection, judge that aspects such as disease carrying germ development degree, O﹠A result of treatment all have important medical value.
Electrochemical immunosensor has unique advantages such as automaticity height, cheapness, easily preparation, rapid sensitive, cooperates immunoreactive efficiently single-mindedly, is fit to very much the analyser of the multiple immunoassay detection of structure serious infectious diseases.The micro-fluidic chip (lab-on-chip) that the title of " life science integrated circuit " is arranged is the desirable technique that makes up microminiaturized, integrated electrochemical immunosensor.The micro-fluidic chip technology be with sampling, pre-service, add reagent, reaction, separation, detection etc. be integrated in finish on the microchip one in front of the door along technology, have analysis speed fast, contain much information, reagent consumption less, advantage such as pollution is little, operation cost is low, instrument is easy to use.And be fit to very much suitability for industrialized production.On behalf of the 21 century analytical instrument, microflow control technique move towards microminiaturized, integrated developing direction.The micro-fluidic Amperometric Detection Coupled chip that the serious infectious diseases that are suitable for structure detect has following advantage: (1) is easy to integrated a plurality of sense channels and detecting electrode on chip by micro-processing technology, accomplishes that multiple target objects analyzes the technical feasibility height simultaneously.(2) by the micro-flowing injection technology, sample flows in pipeline and has guaranteed that electrode surface upgrades constantly, and it is easily contaminated better to have overcome electrode, causes false positive rate height, problem such as collimation difference as a result.(3) microfluidic sensing device upper pipeline diameter has only μ m level, and the reaction tank volume also has only μ L level, and required reagent dosage is few during analysis, and analyte arrives the diffusion length of electrode surface lacks, therefore can significantly reduce the incubation time, realizes the fast joint inspection target.(4) the microfluidic sensing device is many based on the optical detection system at present, and the expensive volume of instrument is big, fully integrated being difficult to, and also it is very high to detect cost, has limited its popularization.And adopting Electrochemical Detection in the sensor, volume is little, automaticity is high, cost is low.
At present, in the micro-fluidic chip technical field, utilize that the multiple specific antigen of syphilis detects simultaneously, the correlation technique and the method for the multiple syphilis antibody of quick diagnosis are not appeared in the newspapers as yet.
Summary of the invention
Technical matters to be solved by this invention is, in the so total technological frame of micro-fluidic chip technology, develop a kind of can utilize the multiple specific antigen of syphilis come to multiple syphilis antibody detect simultaneously, the micro-fluidic chip special and the detection method thereof of quick diagnosis.
The present invention solve the technical problem by following scheme, the device that this scheme provides is a kind of syphilis-diagnosis special multichannel micro-fluidic chip based on electrode modification technology, the structure of this micro-fluidic chip comprises that applying is installed in two plate objects together, described two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip, on the position of the mutual applying between described two plate objects, be equiped with pipeline, and, three pond shape things, one end of pipeline via the manifold shape fluid passage respectively with wherein two pond shape thing UNICOMs, the other end of pipeline and a remaining pond shape thing UNICOM, and, be installed in the described pipeline working electrode on the diverse location in regular turn respectively and to electrode and contrast electrode, described working electrode by conductive electrode and be attached to embedding on the described conductive electrode gold size sensitive membrane of syphilis specific antigen constitute, this case special feature is, the structure of described pipeline is structure in parallel, the described pipeline that is structure in parallel is made of four lateral parallel connections, and, the quantity of described working electrode is four, the installation position of these four working electrodes lays respectively in described four laterals, and, specific antigen material in its top layer gold size sensitive membrane structure of these four working electrodes is respectively four kinds of syphilis antigenic substances to the combination of syphilis antibody energy specificity, these four kinds of antigenic substances are respectively syphilis specific antigen TP0684, TP0453, TP0821 and TP0319, and its pattern of each working electrode all is beading.
Described pipeline in the described microfluidic chip structure and described lateral and described manifold shape fluid passage, its internal diameter size all can be selected arbitrarily size, but, for as far as possible less with the consideration of aspects such as liquid sample to be measured and reduction reagent loss, described pipeline and described lateral and described manifold shape fluid passage are preferably all selected the passage of capillary level for use, the passage meaning of described capillary level is a capillary channel, and the internal diameter capillaceous on its internal diameter and the ordinary meaning is suitable.The shape of cross section of its inner passage of described kapillary can be a shape arbitrarily, described shape of cross section is for example circular, oval, square, rectangle, bar shaped, can certainly be have bending arbitrarily linear, and, described interior shape capillaceous is along with the extension of pipeline, and it is different shapes that the shape of cross section of different parts also can allow.Only with regard to kapillary one speech, its art-recognized meanings is known.
Described gold size sensitive membrane is that shitosan gold size solution and syphilis specific antigenic solution are fully mixed, and is sprayed on the structure position of setting, and its drying and forming-film is formed.Described gold size sensitive membrane has been included as fixing above-mentioned each syphilis specific antigen and has introduced wherein complementary medium, and described complementary medium is wherein a kind of of shitosan, cellulose acetate, gelatin or their potpourri for example.
What relate in the structure is minute sized electrode to electrode and contrast electrode, and its electrode shape all can be selected arbitrarily shape, and described selected arbitrarily shape is square shape sheet shape, rectangle sheet, strip or round sheet or the like for example.
Working electrode in this case chip is beading, its beading electrode profile helps to expand significantly its effective working interface, and, further, the beading pattern also makes this working electrode have good flow-disturbing, and this helps the forced working electrode and the contacting between its peripheral measured object liquid stream of flowing through.
Relate to several pond shape things in this case microfluidic chip structure, described pond shape thing is pond shape or the scrotiform structure that is used for transitional liquid storage, its shape of inner chamber of each pond shape thing wherein all can be selected arbitrarily shape, and described cavity shape is cylindrical cavity shape, square column type cavity-like, oblong cavity shape or spherical hollow space shape or the like for example.The size of described pond shape thing can be arbitrarily selected size, still, for can be as far as possible less with liquid sample to be measured and reduce reagent loss, the hypomegetic pond shape thing that described pond shape thing preferably can mate with kapillary.
This case device can further include some annexes certainly, and described annex is multiple tracks electrochemical workstation and microfluidic pump or the like for example, and the art-recognized meanings of the art-recognized meanings of described multiple tracks electrochemical workstation and microfluidic pump is known.Each working electrode that relates in this case microfluidic chip structure and to electrode and contrast electrode etc., can get lines crossed via corresponding special use is respectively connecting with the corresponding interface of described multiple tracks electrochemical workstation.It is to be used for the private cable that each the corresponding interface with each described electrode and described multiple tracks electrochemical workstation is coupled to each other that described special use is being got lines crossed.Described microfluidic pump is exclusively used in and drives micro liquid and flow, described microfluidic pump can with the described pond of selected any one shape thing UNICOM as required.
Described pattern is catenate working electrode, both can be the beading conductive electrode of integrated compression moulding, also can be the beading conductive electrode of moulding by casting; It is the magnetic bead string structure of electric conductivity that its structure of described working electrode can certainly allow, under this situation, it is the tri-iron tetroxide material with conductive capability that described pattern is its material of catenate working electrode, and it is to add the accurate one-dimensional electric electrode that is spliced mutually under the magnetic field condition guiding by the magnetic bead of many tri-iron tetroxide materials that described pattern is catenate working electrode.
Described pattern is catenate working electrode, it is the working electrode of described magnetic bead string structure, magnetic bead diameter in its structure is not limit, the diameter of described magnetic bead can be the size of setting as required arbitrarily, but, the preferred diameter range of described magnetic bead is between 20 nanometer to 2000 nanometers, and, it is the number of setting as required arbitrarily that the number of the magnetic bead that is stitched together mutually that is contained in the structure of each described working electrode can allow, described number is not limit, but the preferred value of the number of the magnetic bead of a described working electrode of formation is between 100 to 100000.
In view of each described beading working electrode occupation space width less, this working electrode can allow directly to be placed in the capillary channel, narrow relatively fluid passage more helps representing the disturbance ability to peripheral working fluid of beading electrode, and, also help the potentiality of its effective working interface given full play of.
Be installed in the described gold size sensitive membrane of described working electrode surface layer by spraying or the coating of point sample instrument point sample or other appropriate process, it is the thickness of any sample measuring liquid treated generation electrical signals response of setting that its thicknesses of layers can allow, but the in other words conj.or perhaps preferred thickness of the thickness of recommendation is between 10 nanometers and 200 nanometers.
The using method of this case micro-fluidic chip:
Employing adds Micropump and drives the steady flow in the capillary channel of four-way micro-fluidic chip of liquid stream, utilizes the four-way electrochemical analytical instrument respectively four kinds of controller used in syphilis diagnosis antibody to be detected.
The concrete detection of this case micro-fluidic chip uses step as follows:
1, add blood serum sample liquid in the microtubule road, adding under the Micropump driving, various syphilis antibody molecules are by the corresponding syphilis specific antigen capture of gold size sensitive membrane embedding on the electrode surface in each passage.
2, the ELIAS secondary antibody that adds horseradish peroxidase-labeled forms immune complex by sandwich method desmoenzyme mark " two is anti-".
3, adopt the hyperchannel electroanalyzer, add electron mediators such as catechol, adopt ampere method detection " two is anti-" to go up enzyme and cause H
2O
2The electric current of reduction changes, and obtains the kind and the content of each analyte thus.
4, the result is carried out analysis-by-synthesis, syphilis antibody is carried out comprehensive diagnos.
Be necessary that doing one here seems unnecessary supplementary notes: antibody and antigen be relative to each other but different materials, the mutual relationship between them, metaphor more popularly is like being the lock in the cover lockset and the relation of key.
Advantage of the present invention is, integrated four beading working electrodes that are coated with four kinds of syphilis specific antigenic substances respectively on a micro-fluidic chip, these four beading working electrodes are respectively at four kinds of feature diagnosis antibody that detect syphilis, this case micro-fluidic chip is a kind of controller used in syphilis diagnosis micro-fluidic chip special that can utilize the multiple specific antigen of syphilis to detect simultaneously, the design feature of its integrated structure has determined the use of this chip to help to accelerate controller used in syphilis diagnosis speed, improve accuracy rate of diagnosis, reduce the controller used in syphilis diagnosis expense, its beading pattern of the working electrode in the chip structure also helps the forced working electrode to contact with chemistry with detected sample liquid stream impact flow each other.
Description of drawings
Fig. 1 is this case micro-fluidic chip embodiment organigram, what showed is the form of the perspective under the depression angle of this example structure, do not depict described annex among the figure, detailed the describing of yet the CONSTRUCTED SPECIFICATION of described beading working electrode not being amplified among the figure.
Among the figure, 1,2,10 is respectively three pond shape things that installation position is different, the 3rd, the manifold shape fluid passage, 4,7,11,14 is respectively different but four laterals of formation parallel with one another UNICOM in parallel structure of installation position, the 5th, the specific antigen material that is installed in its top layer gold size sensitive membrane structure in the lateral 4 is the beading working electrode of syphilis specific antigen TP0684, the 6th, the specific antigen material that is installed in its top layer gold size sensitive membrane structure in the lateral 7 is the beading working electrode of syphilis specific antigen TP0453, the 12nd, the specific antigen material that is installed in its top layer gold size sensitive membrane structure in the lateral 11 is the beading working electrode of syphilis specific antigen TP0821, the 13rd, the specific antigen material that is installed in its top layer gold size sensitive membrane structure in the lateral 14 is the beading working electrode of syphilis specific antigen TP0319, the 8th, to electrode, the 9th, contrast electrode.
Embodiment
In this case embodiment that Fig. 1 showed, the structure of this micro-fluidic chip comprises that applying is installed in two plate objects together, two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip, on the position of the mutual applying between two plate objects, be equiped with pipeline, and, three pond shape things, these three pond shape things are respectively pond shape things 1, pond shape thing 2 and pond shape thing 10, one end of described pipeline via manifold shape fluid passage 3 respectively with pond shape thing 1 and pond shape thing 2 UNICOMs, the other end of described pipeline and remaining pond shape thing 10 UNICOMs, and, be installed in the described pipeline working electrode on the diverse location in regular turn respectively and to electrode 8 and contrast electrode 9, working electrode by conductive electrode and be attached to embedding on the described conductive electrode gold size sensitive membrane of syphilis specific antigen constitute, the structure of described pipeline is structure in parallel, this pipeline that is structure in parallel is made of four lateral parallel connections, these four laterals are respectively lateral 4 and lateral 7 and lateral 11 and lateral 14, and, the quantity of beading working electrode is four, these four beading working electrodes are respectively beading working electrode 5 and beading working electrode 6 and beading working electrode 12 and beading working electrode 13, wherein, beading working electrode 5 is that the specific antigen material in its top layer gold size sensitive membrane structure that is installed in the lateral 4 is the beading working electrode of syphilis specific antigen TP0684, beading working electrode 6 is that the specific antigen material in its top layer gold size sensitive membrane structure that is installed in the lateral 7 is the beading working electrode of syphilis specific antigen TP0453, beading working electrode 12 is that the specific antigen material in its top layer gold size sensitive membrane structure that is installed in the lateral 11 is the beading working electrode of syphilis specific antigen TP0821, and beading working electrode 13 is that the specific antigen material in its top layer gold size sensitive membrane structure that is installed in the lateral 14 is the beading working electrode of syphilis specific antigen TP0319.Do not draw among Fig. 1 as associate members such as the microfluidic pump of annex and multiple tracks electrochemical workstations, do not have the CONSTRUCTED SPECIFICATION of detailed displaying beading working electrode yet.Each pond shape thing in this routine structure can be as required with as the microfluidic pump of annex UNICOM by any means.Each working electrode in this routine structure and to electrode and contrast electrode can be respectively via special-purpose separately cable or say get lines crossed respectively with as the corresponding cable interface of the multiple tracks electrochemical workstation of annex or say the interface of getting lines crossed and connect.
Claims (5)
1. based on the syphilis-diagnosis special multichannel micro-fluidic chip of electrode modification technology, the structure of this micro-fluidic chip comprises that applying is installed in two plate objects together, described two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip, on the position of the mutual applying between described two plate objects, be equiped with pipeline, and, three pond shape things, one end of pipeline via the manifold shape fluid passage respectively with wherein two pond shape thing UNICOMs, the other end of pipeline and a remaining pond shape thing UNICOM, and, be installed in the described pipeline working electrode on the diverse location in regular turn respectively and to electrode and contrast electrode, described working electrode by conductive electrode and be attached to embedding on the described conductive electrode gold size sensitive membrane of syphilis specific antigen constitute, it is characterized in that, the structure of described pipeline is structure in parallel, the described pipeline that is structure in parallel is made of four lateral parallel connections, and, the quantity of described working electrode is four, the installation position of these four working electrodes lays respectively in described four laterals, and, specific antigen material in its top layer gold size sensitive membrane structure of these four working electrodes is respectively four kinds of syphilis antigenic substances to the combination of syphilis antibody energy specificity, these four kinds of antigenic substances are respectively syphilis specific antigen TP0684, TP0453, TP0821 and TP0319, and its pattern of each working electrode all is beading.
2. the syphilis-diagnosis special multichannel micro-fluidic chip based on electrode modification technology according to claim 1 is characterized in that, described pipeline and described lateral and described manifold shape fluid passage are capillary channel.
3. the syphilis-diagnosis special multichannel micro-fluidic chip based on electrode modification technology according to claim 1, it is characterized in that, it is the tri-iron tetroxide material with conductive capability that described pattern is its material of catenate working electrode, and it is to add the accurate one-dimensional electric electrode that is spliced mutually under the magnetic field condition guiding by the magnetic bead of many tri-iron tetroxide materials that described pattern is catenate working electrode.
4. the syphilis-diagnosis special multichannel micro-fluidic chip based on electrode modification technology according to claim 3, it is characterized in that, the diameter of described magnetic bead is between 20 nanometer to 2000 nanometers, and the number of the magnetic bead that is stitched together mutually that is contained in the structure of each described working electrode is between 100 to 100000.
5. the syphilis-diagnosis special multichannel micro-fluidic chip based on electrode modification technology according to claim 1 is characterized in that, the thickness of described gold size sensitive membrane is between 10 nanometers and 200 nanometers.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel micro-fluidic chip for simultaneously detecting various subtype swine influenza viruses |
| CN104748656A (en) * | 2015-03-20 | 2015-07-01 | 江苏大学 | Micro-fluidic magnetic bead detection method and device based on giant magneto-resistance |
| CN106153931A (en) * | 2015-03-11 | 2016-11-23 | 宁波大学 | The controller used in syphilis diagnosis micro fluidic device that liquid stream driving adapter is easily-disassembled |
| CN106153930A (en) * | 2015-03-11 | 2016-11-23 | 宁波大学 | The controller used in syphilis diagnosis device that a kind of sample driving adapter can fast remove |
| CN107121477A (en) * | 2016-02-25 | 2017-09-01 | 葛宇杰 | The controller used in syphilis diagnosis micro flow control chip device of operation principles is coupled based on dual drive |
| CN107335483A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | The multichannel micro-fluidic chip device of a variety of hypotype swine flus is detected simultaneously |
| CN107335482A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | Cheap controller used in syphilis diagnosis micro fluidic device based on rigid interface modification theory |
| CN107340392A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | Using the controller used in syphilis diagnosis of hard modifying interface transmission liquid stream with cheap micro fluidic device |
| CN107367537A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | The controller used in syphilis diagnosis device that sample driving can be removed fast with function element |
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2009
- 2009-06-19 CN CN200910151217A patent/CN101625359A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel micro-fluidic chip for simultaneously detecting various subtype swine influenza viruses |
| CN106153931A (en) * | 2015-03-11 | 2016-11-23 | 宁波大学 | The controller used in syphilis diagnosis micro fluidic device that liquid stream driving adapter is easily-disassembled |
| CN106153930A (en) * | 2015-03-11 | 2016-11-23 | 宁波大学 | The controller used in syphilis diagnosis device that a kind of sample driving adapter can fast remove |
| CN104748656A (en) * | 2015-03-20 | 2015-07-01 | 江苏大学 | Micro-fluidic magnetic bead detection method and device based on giant magneto-resistance |
| CN107121477A (en) * | 2016-02-25 | 2017-09-01 | 葛宇杰 | The controller used in syphilis diagnosis micro flow control chip device of operation principles is coupled based on dual drive |
| CN107335483A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | The multichannel micro-fluidic chip device of a variety of hypotype swine flus is detected simultaneously |
| CN107335482A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | Cheap controller used in syphilis diagnosis micro fluidic device based on rigid interface modification theory |
| CN107340392A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | Using the controller used in syphilis diagnosis of hard modifying interface transmission liquid stream with cheap micro fluidic device |
| CN107367537A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | The controller used in syphilis diagnosis device that sample driving can be removed fast with function element |
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Application publication date: 20100113 |