CN102478582A - Multi-channel micro-fluidic chip for simultaneously detecting various subtype swine influenza viruses - Google Patents
Multi-channel micro-fluidic chip for simultaneously detecting various subtype swine influenza viruses Download PDFInfo
- Publication number
- CN102478582A CN102478582A CN2011103111271A CN201110311127A CN102478582A CN 102478582 A CN102478582 A CN 102478582A CN 2011103111271 A CN2011103111271 A CN 2011103111271A CN 201110311127 A CN201110311127 A CN 201110311127A CN 102478582 A CN102478582 A CN 102478582A
- Authority
- CN
- China
- Prior art keywords
- fluidic chip
- swine influenza
- influenza virus
- micro
- electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000725681 Swine influenza virus Species 0.000 title claims abstract description 59
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000010931 gold Substances 0.000 claims abstract description 20
- 229910052737 gold Inorganic materials 0.000 claims abstract description 20
- 239000012528 membrane Substances 0.000 claims abstract description 19
- 239000000463 material Substances 0.000 claims description 23
- 239000000427 antigen Substances 0.000 claims description 12
- 102000036639 antigens Human genes 0.000 claims description 12
- 108091007433 antigens Proteins 0.000 claims description 12
- 239000011324 bead Substances 0.000 claims description 11
- 239000000758 substrate Substances 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 9
- 238000009434 installation Methods 0.000 claims description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 4
- 229910052742 iron Inorganic materials 0.000 claims description 4
- 201000010740 swine influenza Diseases 0.000 claims description 3
- 206010069767 H1N1 influenza Diseases 0.000 claims description 2
- 208000009620 Orthomyxoviridae Infections Diseases 0.000 claims description 2
- 241000625014 Vir Species 0.000 claims description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 2
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 2
- 238000003745 diagnosis Methods 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 7
- 238000004458 analytical method Methods 0.000 abstract description 4
- 238000012360 testing method Methods 0.000 abstract description 3
- 239000002344 surface layer Substances 0.000 abstract description 2
- 239000003292 glue Substances 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 description 12
- 239000010410 layer Substances 0.000 description 8
- 239000007788 liquid Substances 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 241000700605 Viruses Species 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 208000035473 Communicable disease Diseases 0.000 description 2
- 101710154606 Hemagglutinin Proteins 0.000 description 2
- 101000905241 Mus musculus Heart- and neural crest derivatives-expressed protein 1 Proteins 0.000 description 2
- 101001031591 Mus musculus Heart- and neural crest derivatives-expressed protein 2 Proteins 0.000 description 2
- 101710093908 Outer capsid protein VP4 Proteins 0.000 description 2
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 2
- 101710176177 Protein A56 Proteins 0.000 description 2
- 230000000890 antigenic effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 239000000185 hemagglutinin Substances 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- CERZMXAJYMMUDR-QBTAGHCHSA-N 5-amino-3,5-dideoxy-D-glycero-D-galacto-non-2-ulopyranosonic acid Chemical compound N[C@@H]1[C@@H](O)CC(O)(C(O)=O)O[C@H]1[C@H](O)[C@H](O)CO CERZMXAJYMMUDR-QBTAGHCHSA-N 0.000 description 1
- 101710128063 Carbohydrate oxidase Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000712431 Influenza A virus Species 0.000 description 1
- 241001500351 Influenzavirus A Species 0.000 description 1
- 102000029749 Microtubule Human genes 0.000 description 1
- 108091022875 Microtubule Proteins 0.000 description 1
- 102000005348 Neuraminidase Human genes 0.000 description 1
- 108010006232 Neuraminidase Proteins 0.000 description 1
- 241000712464 Orthomyxoviridae Species 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- SMEGJBVQLJJKKX-HOTMZDKISA-N [(2R,3S,4S,5R,6R)-5-acetyloxy-3,4,6-trihydroxyoxan-2-yl]methyl acetate Chemical compound CC(=O)OC[C@@H]1[C@H]([C@@H]([C@H]([C@@H](O1)O)OC(=O)C)O)O SMEGJBVQLJJKKX-HOTMZDKISA-N 0.000 description 1
- 229940081735 acetylcellulose Drugs 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000000748 compression moulding Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000835 electrochemical detection Methods 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000011796 hollow space material Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000037797 influenza A Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000003771 laboratory diagnosis Methods 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000004688 microtubule Anatomy 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- CERZMXAJYMMUDR-UHFFFAOYSA-N neuraminic acid Natural products NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO CERZMXAJYMMUDR-UHFFFAOYSA-N 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the field of analysis and test, in particular to a multichannel microfluidic chip for simultaneously detecting multiple subtype Swine Influenza Viruses (SIV). Rapid and inexpensive diagnosis of SIV is one of the goals of advances in related medical technology, and the present invention provides a diagnostic device directed to the above-mentioned goals. The key point of the scheme is that the device also comprises a pipeline which is in a parallel structure in the microfluidic chip, the parallel structure comprises three branch pipelines which are mutually connected in parallel, three beaded working electrodes are arranged in the three branch pipelines respectively, the beaded working electrodes are composed of conductive electrodes and gold glue sensitive membranes which are attached to the conductive electrodes and are embedded with SIV specific antibodies of different subtypes, the respective gold glue sensitive membranes on the surface layers of the three beaded working electrodes are respectively embedded with SIV specific antibody substances of three different subtypes, and the SIV specific antibody substances of the three different subtypes are respectively subtype H1N1、H3N2And H1N2。
Description
Technical field
The present invention relates to a kind of multichannel micro-fluidic chip that detects multiple hypotype swine influenza virus simultaneously, this swine influenza virus diagnostic device is based on the micro-fluidic chip special that the antigen/antibody specific reaction is diagnosed swine influenza virus antigen, belongs to the analytical test field.
Background technology
(Swineinfluenza vires SIV) belongs to orthomyxoviridae family's influenza A virus and belongs to (Influenza virus A) swine influenza virus, is the sub-thread minus-stranded rna virus, and its genome is made up of 8 independent segments that differ in size.The hypotype of Flu-A is to divide according to the different of hemagglutinin (Hemagglutinin) and neuraminic acid (Neuraminidase), and it can be divided into 15 kinds of N antigenic subtypes that different H antigenic subtypes is different with 9 kinds.Cause world pop SIV serotype hypotype to mainly contain 3 kinds at present: H
1N
1, H
3N
2And H
1N
2Be master's global first type H with the Mexico and the U.S. recently
1N
1Influenza break out more firm to research and the prevention and control of SIV.Therefore it is very urgent with the method for making a definite diagnosis SIV to develop quick diagnosis, aspect reducing the world economy loss and improving human hygiene and health, all has profound significance.
Present traditional swine flu diagnostic method; Be broadly divided into four kinds: the one, virus is separated, and virus is separated the standard of being regarded as laboratory diagnosis, but shortcoming is that institute's time-consuming is longer; And can only carry out in special laboratory, often can not prevent in time that the system measure provides foundation; The 2nd, detect viral nucleic acid, the most frequently used is reverse transcription PCR, many Auele Specific Primer is carried out multiple PCR if utilize, and can realize that different subtype SIV detects simultaneously, but influence factor is many, complicated operation, consuming time, needs the professional and technical personnel to operate simultaneously; The 3rd, detect virus protein, like fluorescence antibody method and SABC method, though the needs that can satisfy quick diagnosis of these class methods, its sensitivity is low, specificity is not strong; The 4th, utilize immunological method to detect the specific antibody in the serum, like EUSA (ELISA); Though higher sensitivity and specificity are arranged, a spot of false negative and false positive are also arranged.
Electrochemical immunosensor has automaticity height, cheapness, is prone to unique advantages such as preparation, rapid sensitive, cooperates immunoreactive efficiently single-mindedly, is fit to very much the analyser of the multiple immunoassay detection of structure serious infectious diseases.The micro-fluidic chip (lab-on-chip) that the title of " life science integrated circuit " is arranged is the desirable technique that makes up microminiaturized, integrated electrochemical immunosensor.The micro-fluidic chip technology be with sampling, pre-service, add reagent, reaction, separation, detection etc. be integrated in accomplish on the microchip one in front of the door along technology, have analysis speed fast, contain much information, reagent consumption less, advantage such as pollution is little, operation cost is low, instrument is easy to use.And be fit to very much suitability for industrialized production.On behalf of the 21 century analytical instrument, microflow control technique move towards microminiaturized, integrated developing direction.The micro-fluidic Amperometric Detection Coupled chip that the serious infectious diseases that are suitable for structure detect has following advantage: (1) is easy to integrated a plurality of sense channels and detecting electrode on chip through micro-processing technology, accomplishes that multiple target objects analyzes simultaneously, and technical feasibility is high.(2) through micro-flowing injection technology, sample flows in pipeline and has guaranteed that electrode surface upgrades constantly, has better overcome electrode and has been prone to contaminatedly, causes high, the problem such as collimation difference as a result of false positive rate.(3) microfluidic sensing device upper pipeline diameter has only μ m level, and the reaction tank volume also has only μ L level, and required reagent dosage is few during analysis, and analyte arrives the diffusion length of electrode surface lacks, therefore can significantly reduce the incubation time, realizes the fast joint inspection target.(4) the microfluidic sensing device is many based on the optical detection system at present, and the expensive volume of instrument is big, fully integrated being difficult to, and also it is very high to detect cost, has limited its popularization.And adopting Electrochemical Detection in the sensor, volume is little, automaticity is high, cost is low.
At present, in the micro-fluidic chip technical field, utilize that the specific antibody of the multiple hypotype of swine influenza virus (SIV) detects simultaneously, the correlation technique and the method for the multiple hypotype SIV antigen of quick diagnosis are not appeared in the newspapers as yet.
Summary of the invention
Technical matters to be solved by this invention is; In the so total technological frame of micro-fluidic chip technology, develop a kind of specific antibody that can utilize many kinds of hypotypes of SIV come to corresponding SIV antigen detect simultaneously, the micro-fluidic chip special of quick diagnosis.
The present invention solve the technical problem through following scheme, and the device that this scheme provides is a kind of multichannel micro-fluidic chip that detects multiple hypotype SIV simultaneously, and the structure of this micro-fluidic chip comprises that applying is installed in two plate objects together; Said two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip; On the position of the mutual applying between said two plate objects, be equiped with pipeline, and, three pond shape things; One end of pipeline via the manifold shape fluid passage respectively with wherein two pond shape thing UNICOMs; The other end of pipeline and a remaining pond shape thing UNICOM, and, be installed in the said pipeline working electrode on the diverse location in regular turn respectively and to electrode and contrast electrode; Said working electrode by conductive electrode and be attached to embedding on the said conductive electrode gold size sensitive membrane of SIV specific antibody constitute; This case special feature is that the structure of said pipeline is the parallel connection structure, and the said pipeline that is the parallel connection structure is made up of three lateral parallel connections; And; The quantity of said working electrode is three, and the installation position of these three working electrodes lays respectively in said three laterals, and; Specific antibody in its top layer gold size sensitive membrane structure of these three working electrodes is respectively three kinds of different subtype SIV antibody materials that different subtype SIV antigen ability specificity is combined, and the specific antibody material of these three kinds of different subtypes is respectively H
1N
1, H
3N
2And H
1N
2, and its pattern of each working electrode all is beading.
Said gold size sensitive membrane is that shitosan gold size solution and SIV specific anti liquid solution are fully mixed, and its drying and forming-film is formed.Different subtype SIV in the said gold size sensitive membrane is the SIV antibody of horseradish peroxidase or glucose carbohydrate oxidase mark; Said gold size sensitive membrane has been included as fixing above-mentioned each SIV specific antibody and has introduced complementary medium wherein, and said complementary medium is wherein a kind of of shitosan, acetyl cellulose, gelatin or their potpourri for example.
Said pipeline in the said microfluidic chip structure and said lateral and said manifold shape fluid passage; Its internal diameter size all can be selected arbitrarily size; But less with the consideration of aspects such as liquid appearance to be measured and reduction reagent loss, said pipeline and said lateral and said manifold shape fluid passage are preferably all selected the passage of capillary level for use from as far as possible; The passage meaning of said capillary level is a capillary channel, and the internal diameter capillaceous on its internal diameter and the ordinary meaning is suitable.The shape of cross section of its inner passage of said kapillary can be a shape arbitrarily; Said shape of cross section is for example circular, oval, square, rectangle, bar shaped; Can certainly be have bending arbitrarily linear; And said interior shape capillaceous is along with the extension of pipeline, and it is different shapes that the shape of cross section of different parts also can allow.Only with regard to kapillary one speech, its art-recognized meanings is known.
What relate in the structure is minute sized electrode to electrode and contrast electrode, and its electrode shape all can be selected arbitrarily shape, and said selected arbitrarily shape is square shape sheet shape, rectangle sheet, strip or round sheet or the like for example.
Working electrode in this case chip is beading; Its beading electrode profile helps to expand significantly its effective working interface; And; Further, the beading pattern also makes this working electrode have good flow-disturbing property, and this helps the forced working electrode and the contacting between its peripheral measured object flow of flowing through.
Relate to several pond shape things in this case microfluidic chip structure; Said pond shape thing is pond shape or the scrotiform structure that is used for transitional liquid storage; Its shape of inner chamber of each pond shape thing wherein all can be selected arbitrarily shape, and said cavity shape is cylindrical cavity shape, square column type cavity-like, oblong cavity shape or spherical hollow space shape or the like for example.The size of said pond shape thing can be arbitrarily selected size, still, for can be as far as possible less with liquid appearance to be measured and reduce reagent loss, the hypomegetic pond shape thing that said pond shape thing preferably can mate with kapillary.
This case device can further include some annexes certainly, and said annex is multiple tracks electrochemical workstation and microfluidic pump or the like for example, and the art-recognized meanings of the art-recognized meanings of said multiple tracks electrochemical workstation and microfluidic pump is known.Each beading working electrode that relates in this case microfluidic chip structure and to electrode and contrast electrode etc., can get lines crossed via corresponding special use is respectively connecting with the corresponding interface of said multiple tracks electrochemical workstation.It is to be used for the private cable that each the corresponding interface with each said electrode and said multiple tracks electrochemical workstation is coupled to each other that said special use is being got lines crossed.Said microfluidic pump is exclusively used in and drives micro liquid and flow, said microfluidic pump can with selected any said pond shape thing UNICOM as required.
Said pattern is catenate working electrode, both can be the beading conductive electrode of integrated compression moulding, also can be the beading conductive electrode of moulding by casting; It is the magnetic bead string structure of electric conductivity that its structure of said working electrode can certainly allow; Under this situation; It is the tri-iron tetroxide material with conductive capability that said pattern is its material of catenate working electrode; And it is to add the accurate one-dimensional electric property electrode that is spliced each other under the magnetic field condition guiding by the magnetic bead of many tri-iron tetroxide materials that said pattern is catenate working electrode.
Said pattern is catenate working electrode, the working electrode of promptly said magnetic bead string structure, and the magnetic bead diameter in its structure is not limit; The diameter of said magnetic bead can be the size of setting as required arbitrarily; But the preferred diameter range of said magnetic bead is between 20 nanometer to 2000 nanometers, and; It is the number of setting as required arbitrarily that the number of the magnetic bead that is stitched together each other that is contained in the structure of each said working electrode can allow; Said number is not limit, and still, the preferred value of the number of the magnetic bead of a said working electrode of formation is between 100 to 100000.
Seeing that each said beading working electrode occupation space width is less; This working electrode can allow directly to be placed in the capillary channel; Narrow relatively fluid passage more helps representing the disturbance ability to peripheral working fluid of beading electrode; And, also help the potentiality of its effective working interface given full play of.
Be installed in the said gold size sensitive membrane of said working electrode surface layer through spraying or the coating of point sample instrument point sample or other appropriate process; It is the thickness of any sample measuring liquid treated generation electrical signals response of setting that its thicknesses of layers can allow; But the in other words conj.or perhaps preferred thickness of the thickness of recommendation is between 10 nanometers and 200 nanometers.
It is any electrical insulating property material that said cover plate and substrate in the chip structure, its material can allow, for example: and polypropylene, glass, or the like, preferred material is a polymethylmethacrylate.
It is the width value that can reach any setting of dependence test purpose that said cover plate in the structure and its width of substrate can allow, and still, preferred said width value is all between 2 centimetres and 4 centimetres; It is the length value of setting arbitrarily that can reach the dependence test purpose that the length value of said cover plate and substrate can allow too, and still, preferred length value or the length value of saying recommendation are between 3 centimetres and 6 centimetres.
It is the thickness of setting arbitrarily of being convenient to the device assembling that its thickness of said cover plate and substrate can allow, the thickness of recommendation or to say preferred thickness be between 0.5 millimeter and 5 millimeters.Less thickness helps saving material.
The method of application of this case micro-fluidic chip:
Employing adds Micropump and drives flow steady flow in the capillary channel of triple channel micro-fluidic chip, utilizes the triple channel electrochemical analytical instrument respectively different subtype SIV diagnostic antigen to be detected.
The concrete detection of this case micro-fluidic chip uses step following:
1, in the microtubule road, add blood serum sample liquid, adding under the Micropump driving, various different subtype SIV antigen molecules are caught by the SIV specific antibody of the corresponding horseradish peroxidase-labeled of gold size sensitive membrane embedding on the electrode surface in each passage.
2, the corresponding SIV antigen in the SIV specific antibody of horseradish peroxidase-labeled and the blood serum sample forms immune complex.
3, adopt multi-channel electrochemical analyzer, add electron mediators such as catechol, adopt the ampere method to detect the electric current variation that above-mentioned reaction causes, obtain the kind and the content of various analytes thus.
4, the result is carried out analysis-by-synthesis, different subtype SIV antigen is diagnosed.
Advantage of the present invention is; At a physical device is integrated three beading working electrodes that are coated with the swine influenza virus specific antibody material of three kinds of different subtypes respectively on the micro-fluidic chip; These three beading working electrodes detect to the characteristic antigen of three kinds of different subtype SIV respectively; This case micro-fluidic chip is a kind of micro-fluidic chip that can utilize multiple different subtype SIV specific antibody to detect simultaneously, and the design feature of its integrated structure and special beading working electrode pattern help to improve the swine influenza virus diagnosis efficiency, reduce diagnostic fees usefulness.
Description of drawings
Fig. 1 is this case micro-fluidic chip embodiment organigram; What showed is the form of the perspective under the depression angle of this example structure; Do not depict said annex among the figure, and, the detailed pattern of said beading working electrode is not amplified among the figure yet and describe, show.
Among the figure; 1,2,8 is respectively three pond shape things that installation position is different; The 3rd, the manifold shape fluid passage; 4,9,12 is respectively different but three laterals of the parallelly connected UNICOM of formation parallel with one another structure of installation position, the 5th, and the specific antibody material that is installed in its top layer gold size sensitive membrane structure in the lateral 4 is hypotype H
1N
1The beading working electrode of swine influenza virus specific antibody, the 10th, the specific antibody material that is installed in its top layer gold size sensitive membrane structure in the lateral 9 is hypotype H
3N
2The beading working electrode of swine influenza virus specific antibody, the 11st, the specific antibody material that is installed in its top layer gold size sensitive membrane structure in the lateral 12 is hypotype H
1N
2The beading working electrode of swine influenza virus specific antibody, the 6th, to electrode, the 7th, contrast electrode.
Embodiment
In this case embodiment that Fig. 1 showed; This device also is that the structure of micro-fluidic chip comprises that applying is installed in two plate objects together; Two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip, on the position of the mutual applying between two plate objects, are equiped with pipeline, and; Three pond shape things; These three pond shape things are respectively pond shape thing 1, pond shape thing 2 and pond shape thing 8, an end of said pipeline via manifold shape fluid passage 3 respectively with pond shape thing 1 and pond shape thing 2 UNICOMs, the other end of said pipeline and remaining pond shape thing 10 UNICOMs; And; Be installed in the said pipeline beading working electrode on the diverse location in regular turn respectively and to electrode 6 and contrast electrode 7, the beading working electrode by conductive electrode and be attached to embedding on the said conductive electrode gold size sensitive membrane of swine influenza virus specific antibody antibody constitute, the structure of said pipeline is the parallel connection structure; This pipeline that is the parallel connection structure is made up of three lateral parallel connections; These three laterals are respectively lateral 4 and lateral 9 and lateral 12, and the quantity of beading working electrode is three, and these three beading working electrodes are respectively beading working electrode 5 and beading working electrode 10 and beading working electrode 11; Wherein, beading working electrode 5 is that the specific antibody material in its top layer gold size sensitive membrane structure that is installed in the lateral 4 is swine influenza virus specific antibody H
1N
1The beading working electrode, beading working electrode 10 is that the specific antibody material in its top layer gold size sensitive membrane structure that is installed in the lateral 9 is swine influenza virus specific antibody H
3N
2The beading working electrode, beading working electrode 11 is that the specific antibody material in its top layer gold size sensitive membrane structure that is installed in the lateral 12 is swine influenza virus specific antibody H
1N
2The beading working electrode.Do not draw among Fig. 1 as associate members such as the microfluidic pump of annex and multiple tracks electrochemical workstations.Each pond shape thing in this routine structure can be based on pressing any way UNICOM with the microfluidic pump as annex.Each beading working electrode in this routine structure and to electrode and contrast electrode can be respectively via special-purpose separately cable or get lines crossed respectively and to connect with the corresponding cable interface or the interface of getting lines crossed as the multiple tracks electrochemical workstation of annex.As if be necessary to do a bit extra supplementary notes here: antibody and antigen be relative to each other but different materials, the mutual relationship between them is likened more popularly, like being one to overlap lock and the relation of key in the lockset.
Claims (8)
1. (structure of this micro-fluidic chip comprises that applying is installed in two plate objects together for Swineinfluenza vires, multichannel micro-fluidic chip SIV) to detect multiple hypotype swine influenza virus simultaneously; Said two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip; On the position of the mutual applying between said two plate objects, be equiped with pipeline, and, three pond shape things; One end of pipeline via the manifold shape fluid passage respectively with wherein two pond shape thing UNICOMs; The other end of pipeline and a remaining pond shape thing UNICOM, and, be installed in the said pipeline working electrode on the diverse location in regular turn respectively and to electrode and contrast electrode; Said working electrode by conductive electrode and be attached to embedding on the said conductive electrode gold size sensitive membrane of different subtype swine influenza virus antibody constitute; It is characterized in that the structure of said pipeline is the parallel connection structure, the said pipeline that is the parallel connection structure is made up of three lateral parallel connections; And; The quantity of said working electrode is three, and the installation position of these three working electrodes lays respectively in said three laterals, and; Specific antibody in its top layer gold size sensitive membrane structure of these three working electrodes is respectively three kinds of corresponding swine influenza virus antibody materials that different subtype swine influenza virus antigen ability specificity is combined, and these three kinds of antibody materials are respectively swine influenza virus specific antibody H
1N
1, H
3N
2And H
1N
2, and its pattern of each working electrode all is beading.
2. the multichannel micro-fluidic chip that detects multiple hypotype swine influenza virus simultaneously according to claim 1 is characterized in that, said pipeline and said lateral and said manifold shape fluid passage are capillary channel.
3. the multichannel micro-fluidic chip that detects multiple hypotype swine influenza virus simultaneously according to claim 1; It is characterized in that; It is the tri-iron tetroxide material with conductive capability that said pattern is its material of catenate working electrode; And it is to add the accurate one-dimensional electric property electrode that is spliced each other under the magnetic field condition guiding by the magnetic bead of many tri-iron tetroxide materials that said pattern is catenate working electrode.
4. the multichannel micro-fluidic chip that detects multiple hypotype swine influenza virus simultaneously according to claim 3; It is characterized in that; The diameter of said magnetic bead is between 20 nanometer to 2000 nanometers; And the number of the magnetic bead that is stitched together each other that is contained in the structure of each said working electrode is between 100 to 100000.
5. the multichannel micro-fluidic chip that detects multiple hypotype swine influenza virus simultaneously according to claim 1 is characterized in that, the thickness of said gold size sensitive membrane is between 10 nanometers and 200 nanometers.
6. the multichannel micro-fluidic chip that detects multiple hypotype swine influenza virus simultaneously according to claim 1 is characterized in that, the said cover plate in the structure and its material of substrate are polymethylmethacrylate.
7. the multichannel micro-fluidic chip that detects multiple hypotype swine influenza virus simultaneously according to claim 1 is characterized in that, all between 2 centimetres and 4 centimetres, its length is all between 3 centimetres and 6 centimetres for the said cover plate in the structure and its width of substrate.
8. the multichannel micro-fluidic chip that detects multiple hypotype swine influenza virus simultaneously according to claim 1 is characterized in that, the said cover plate in the structure and its thickness of substrate are all between 0.5 millimeter and 5 millimeters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103111271A CN102478582A (en) | 2011-10-08 | 2011-10-08 | Multi-channel micro-fluidic chip for simultaneously detecting various subtype swine influenza viruses |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103111271A CN102478582A (en) | 2011-10-08 | 2011-10-08 | Multi-channel micro-fluidic chip for simultaneously detecting various subtype swine influenza viruses |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102478582A true CN102478582A (en) | 2012-05-30 |
Family
ID=46091294
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011103111271A Pending CN102478582A (en) | 2011-10-08 | 2011-10-08 | Multi-channel micro-fluidic chip for simultaneously detecting various subtype swine influenza viruses |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102478582A (en) |
Cited By (80)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104483360A (en) * | 2014-12-08 | 2015-04-01 | 中国科学院烟台海岸带研究所 | Potential paper chip and preparation and application thereof |
| CN105879932A (en) * | 2015-01-18 | 2016-08-24 | 宁波大学 | Multi-channel micro-fluidic chip device for simultaneously detecting various kinds of subtype swine flu |
| CN105879934A (en) * | 2015-01-18 | 2016-08-24 | 宁波大学 | Multi-channel micro-fluidic chip device for simultaneous detection of swine flu of multiple subtypes |
| CN106153702A (en) * | 2015-04-23 | 2016-11-23 | 宁波大学 | Coupling Dual Drive detects the micro flow control chip device of multiple hypotype swine flue simultaneously |
| CN106153955A (en) * | 2015-03-18 | 2016-11-23 | 宁波大学 | It is prone to the hypotype swine flue detection device applying cheap material disassembled |
| CN106153697A (en) * | 2015-04-23 | 2016-11-23 | 宁波大学 | Multichannel and the cholera diagnosis micro flow control chip device of Dual Drive coupling running |
| CN106153695A (en) * | 2015-03-31 | 2016-11-23 | 宁波大学 | Comprise the AIDS diagnosis multichannel micro-fluidic chip device of hydrophobic substrate |
| CN106153694A (en) * | 2015-03-31 | 2016-11-23 | 宁波大学 | The hypotype swine flue detection chip apparatus using PDMS to be substrate material |
| CN106153701A (en) * | 2015-04-23 | 2016-11-23 | 宁波大学 | Detect multiple hypotype swine flue Dual Drive CGCM micro flow control chip device |
| CN106153706A (en) * | 2015-03-31 | 2016-11-23 | 宁波大学 | Using hydrophobic material as the AIDS diagnosis device of its substrate of micro-fluidic chip |
| CN106153898A (en) * | 2015-03-11 | 2016-11-23 | 宁波大学 | The additional hypotype swine flue detection device driving liquid stream component to be prone to removal |
| CN106153888A (en) * | 2015-03-11 | 2016-11-23 | 宁波大学 | The hypotype swine flue detection device that fluid drives component can remove rapidly |
| CN106290526A (en) * | 2015-05-11 | 2017-01-04 | 宁波大学 | The hypotype swine flue detection device of the Dual Drive CGCM conveniently disassembled |
| CN106290935A (en) * | 2015-06-11 | 2017-01-04 | 宁波大学 | A kind of hypotype swine flue detection micro flow control chip device using strong hydrophobic substrate |
| CN106290929A (en) * | 2015-05-11 | 2017-01-04 | 宁波大学 | The hypotype swine flue detection device of the Dual Drive CGCM that can disassemble rapidly |
| CN106290931A (en) * | 2015-05-26 | 2017-01-04 | 宁波大学 | The hypotype swine flue detection Multichannel device of substrate is made with cheap hydrophobic material |
| CN106290930A (en) * | 2015-05-26 | 2017-01-04 | 宁波大学 | The hypotype swine flue multi-channel testing device of the Dual Drive coupling containing hydrophobic substrate |
| CN106556708A (en) * | 2015-09-24 | 2017-04-05 | 宁波大学 | The micro flow control chip device containing hydrophobic substrate of two kinds of type of drive coupling runnings |
| CN106556709A (en) * | 2015-09-24 | 2017-04-05 | 宁波大学 | The micro flow control chip device comprising hydrophobic substrate of coupling Dual Drive pattern |
| CN106568953A (en) * | 2015-10-12 | 2017-04-19 | 宁波大学 | Microfluidic chip device for detecting multiple tumor markers based on 15-channel joint detection |
| CN106568952A (en) * | 2015-10-12 | 2017-04-19 | 宁波大学 | Micro-fluidic chip device for simultaneously detecting women's various typical tumor markers |
| CN106610399A (en) * | 2015-10-26 | 2017-05-03 | 宁波大学 | Integrated structure using dual-drive coupled tumor marker combined detection chip apparatus |
| CN106610429A (en) * | 2015-10-26 | 2017-05-03 | 宁波大学 | Dual-drive tumor marker joint inspection chip device with PDMS as substrate |
| CN106680348A (en) * | 2015-11-07 | 2017-05-17 | 宁波大学 | Easy-to-assemble chip device for joint detection of male typical tumor markers |
| CN106680349A (en) * | 2015-11-07 | 2017-05-17 | 宁波大学 | Typical tumor marker combined detection chip device for female medical screening |
| CN106814119A (en) * | 2015-11-30 | 2017-06-09 | 宁波大学 | Physical examination examination male's classifiable tumor mark micro flow control chip device |
| CN106984365A (en) * | 2016-01-20 | 2017-07-28 | 李榕生 | The multichannel micro-fluidic chip device that a variety of hypotype swine flus are detected simultaneously |
| CN106984367A (en) * | 2016-01-20 | 2017-07-28 | 李榕生 | Interphase interface characteristic is relied on to reach the micro flow control chip device of sample sap flow process |
| CN106990242A (en) * | 2016-01-20 | 2017-07-28 | 李榕生 | The multichannel micro-fluidic chip device of a variety of hypotype swine flus is detected simultaneously |
| CN107020158A (en) * | 2016-02-01 | 2017-08-08 | 葛宇杰 | The hypotype swine flu detection device that fluid driving can be removed rapidly with component |
| CN107020160A (en) * | 2016-02-01 | 2017-08-08 | 葛宇杰 | Additional driving liquid stream is easy to the hypotype swine flu detection means of removal with component |
| CN107045060A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | Easily-disassembled hypotype swine flu multi-channel testing device comprising electromagnetism auxiliary part |
| CN107042127A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | The cholera diagnosis micro fluidic device of quick removal is capable of in sample liquid driving with component |
| CN107042126A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | Using the micro flow control chip device of PDMS material substrates |
| CN107045011A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | It is a kind of to be not only easily installed but also be easy to the cholera diagnosis Multichannel device disassembled |
| CN107045059A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | It is easy to the hypotype swine flu detection device for applying cheap material disassembled |
| CN107081179A (en) * | 2016-02-15 | 2017-08-22 | 葛宇杰 | The micro flow control chip device of substrate material is used as using dimethyl silicone polymer |
| CN107085101A (en) * | 2016-02-15 | 2017-08-22 | 葛宇杰 | Strong hydrophobic PDMS as substrate material hypotype swine flu detection chip apparatus |
| CN107085016A (en) * | 2016-02-15 | 2017-08-22 | 葛宇杰 | PDMS is used for the hypotype swine flu detection chip apparatus of substrate material |
| CN107115904A (en) * | 2016-02-25 | 2017-09-01 | 葛宇杰 | Coupling dual drive detects the micro flow control chip device of a variety of hypotype swine flus simultaneously |
| CN107115900A (en) * | 2016-02-25 | 2017-09-01 | 葛宇杰 | For the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter |
| CN107115903A (en) * | 2016-02-25 | 2017-09-01 | 葛宇杰 | Detect a variety of hypotype swine flu dual drive CGCM micro flow control chip devices |
| CN107138185A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | The controller used in syphilis diagnosis micro fluidic device of the dual drive CGCM of easily-disassembled framework |
| CN107138184A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | Driving is with the easily-disassembled and recyclable cholera diagnosis device of component |
| CN107138191A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | The hypotype swine flu detection device for the dual drive CGCM that can be disassembled rapidly |
| CN107138192A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | A kind of easily-disassembled cholera diagnosis micro fluidic device of dual drive CGCM |
| CN107138188A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | The hypotype swine flu detection means for the dual drive CGCM conveniently disassembled |
| CN107167505A (en) * | 2016-03-08 | 2017-09-15 | 葛宇杰 | The hypotype swine flu multi-channel testing device of dual drive coupling containing hydrophobic substrate |
| CN107167599A (en) * | 2016-03-08 | 2017-09-15 | 葛宇杰 | Make the hypotype swine flu detection Multichannel device of substrate with cheap hydrophobic material |
| CN107159322A (en) * | 2016-03-08 | 2017-09-15 | 葛宇杰 | The micro flow control chip device of the substrate containing PDMS based on dual drive CGCM |
| CN107185603A (en) * | 2016-03-15 | 2017-09-22 | 李榕生 | Multichannel micro-fluidic device is used in a kind of strong hydrophobic cholera diagnosis of substrate of its chip |
| CN107185606A (en) * | 2016-03-15 | 2017-09-22 | 李榕生 | A kind of hypotype swine flu detection micro flow control chip device using strong hydrophobic substrate |
| CN107185609A (en) * | 2016-03-15 | 2017-09-22 | 李榕生 | A kind of hypotype swine flu detection micro flow control chip device comprising hydrophobic substrate |
| CN107185607A (en) * | 2016-03-15 | 2017-09-22 | 李榕生 | The micro flow control chip device containing hydrophobic substrate of two kinds of type of drive coupling runnings |
| CN107185608A (en) * | 2016-03-15 | 2017-09-22 | 李榕生 | A kind of substrate material is PDMS cholera diagnosis micro flow control chip device |
| CN107238705A (en) * | 2016-03-29 | 2017-10-10 | 李榕生 | Tumor markers is generally investigated with ten Five-channel micro flow control chip devices |
| CN107335483A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | The multichannel micro-fluidic chip device of a variety of hypotype swine flus is detected simultaneously |
| CN107335484A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | The firmly modified micro flow control chip device of its transmission pipeline of sample liquid stream |
| CN107340391A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | The multichannel micro-fluidic chip device that a variety of hypotype swine flus detect simultaneously |
| CN107344126A (en) * | 2016-05-05 | 2017-11-14 | 李榕生 | The multichannel micro-fluidic chip device that a variety of hypotype swine flus detect simultaneously |
| CN107345959A (en) * | 2016-05-05 | 2017-11-14 | 李榕生 | The multichannel micro-fluidic chip device of a variety of hypotype swine flus is detected simultaneously |
| CN107344127A (en) * | 2016-05-05 | 2017-11-14 | 李榕生 | Interphase interface characteristic is relied on to reach the micro flow control chip device of sample sap flow process |
| CN107349980A (en) * | 2016-05-10 | 2017-11-17 | 李榕生 | The hypotype swine flu detection device that fluid driving can be removed rapidly with component |
| CN107349981A (en) * | 2016-05-10 | 2017-11-17 | 李榕生 | A kind of micro flow control chip device for taking new fluid type of drive |
| CN107349978A (en) * | 2016-05-10 | 2017-11-17 | 李榕生 | Additional driving liquid stream is easy to the hypotype swine flu detection means of removal with component |
| CN107362842A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | The cholera diagnosis micro fluidic device of quick removal is capable of in sample liquid driving with component |
| CN107362841A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | Easily-disassembled hypotype swine flu multi-channel testing device comprising electromagnetism auxiliary part |
| CN107362837A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | Be easy to disassemble applies the hypotype swine flu detection device of cheap material |
| CN107362840A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | Using the micro flow control chip device of PDMS material substrates |
| CN107377015A (en) * | 2016-05-17 | 2017-11-24 | 李榕生 | Use hypotype swine flu detection chip apparatus of the PDMS for substrate material |
| CN107377017A (en) * | 2016-05-17 | 2017-11-24 | 李榕生 | Micro flow control chip device using dimethyl silicone polymer as substrate material |
| CN107377016A (en) * | 2016-05-17 | 2017-11-24 | 李榕生 | Strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material |
| CN107486267A (en) * | 2016-06-13 | 2017-12-19 | 李榕生 | The multichannel micro-fluidic chip device that a variety of hypotype swine flus detect simultaneously |
| CN107656054A (en) * | 2016-07-26 | 2018-02-02 | 洪小女 | The hypotype swine flu detection device that fluid driving can be removed rapidly with component |
| CN107649219A (en) * | 2016-07-26 | 2018-02-02 | 宋岳 | Easily-disassembled hypotype swine flu multi-channel testing device comprising electromagnetism auxiliary part |
| CN107649203A (en) * | 2016-07-26 | 2018-02-02 | 洪小女 | Additional driving liquid stream is easy to the hypotype swine flu detection means of removal with component |
| CN107656052A (en) * | 2016-07-26 | 2018-02-02 | 宋岳 | Be easy to disassemble applies the hypotype swine flu detection device of cheap material |
| CN107649208A (en) * | 2016-07-26 | 2018-02-02 | 宋岳 | Strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material |
| CN107649194A (en) * | 2016-07-26 | 2018-02-02 | 宋岳 | Use hypotype swine flu detection chip apparatus of the PDMS for substrate material |
| CN110082527A (en) * | 2019-04-30 | 2019-08-02 | 杭州艾替捷英科技有限公司 | African swine fever virus chemical luminescence reagent kit |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101581725A (en) * | 2009-06-19 | 2009-11-18 | 宁波大学 | Multichannel micro-fluidic chip specially used for AIDS diagnosis and comprising quasi-one-dimensional sensitive electrodes |
| CN101581724A (en) * | 2009-06-19 | 2009-11-18 | 宁波大学 | Special multichannel syphilis diagnosis device combined with quasi-one-dimensional specific antigen modified electrodes |
| CN101587123A (en) * | 2009-06-19 | 2009-11-25 | 宁波大学 | Special micro-fluidic chip for cholera diagnosis with one-dimensional self-assembly magnetic bead chain electrodes |
| CN101587124A (en) * | 2009-07-12 | 2009-11-25 | 宁波大学 | Micro-fluidic chip special for diagnosing syphilis by aid of organic conductor material technology |
| CN101625359A (en) * | 2009-06-19 | 2010-01-13 | 宁波大学 | Syphilis-diagnosis special multichannel micro-fluidic chip based on electrode modification technology |
| CN101661038A (en) * | 2009-07-12 | 2010-03-03 | 宁波大学 | Special multi-channel micro-flow controller used in syphilis diagnosis and using low-priced conducting material |
| CN201421455Y (en) * | 2008-12-30 | 2010-03-10 | 宁波大学 | Special micro-fluidic chip for syphilis diagnosis |
| CN201449391U (en) * | 2008-12-30 | 2010-05-05 | 宁波大学 | Syphilis diagnosing device containing electrodes covered by gold colloid antigenic sensitive films |
-
2011
- 2011-10-08 CN CN2011103111271A patent/CN102478582A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201421455Y (en) * | 2008-12-30 | 2010-03-10 | 宁波大学 | Special micro-fluidic chip for syphilis diagnosis |
| CN201449391U (en) * | 2008-12-30 | 2010-05-05 | 宁波大学 | Syphilis diagnosing device containing electrodes covered by gold colloid antigenic sensitive films |
| CN101581725A (en) * | 2009-06-19 | 2009-11-18 | 宁波大学 | Multichannel micro-fluidic chip specially used for AIDS diagnosis and comprising quasi-one-dimensional sensitive electrodes |
| CN101581724A (en) * | 2009-06-19 | 2009-11-18 | 宁波大学 | Special multichannel syphilis diagnosis device combined with quasi-one-dimensional specific antigen modified electrodes |
| CN101587123A (en) * | 2009-06-19 | 2009-11-25 | 宁波大学 | Special micro-fluidic chip for cholera diagnosis with one-dimensional self-assembly magnetic bead chain electrodes |
| CN101625359A (en) * | 2009-06-19 | 2010-01-13 | 宁波大学 | Syphilis-diagnosis special multichannel micro-fluidic chip based on electrode modification technology |
| CN101587124A (en) * | 2009-07-12 | 2009-11-25 | 宁波大学 | Micro-fluidic chip special for diagnosing syphilis by aid of organic conductor material technology |
| CN101661038A (en) * | 2009-07-12 | 2010-03-03 | 宁波大学 | Special multi-channel micro-flow controller used in syphilis diagnosis and using low-priced conducting material |
Non-Patent Citations (3)
| Title |
|---|
| 《河南农业大学硕士学位论文》 20061231 段廷云 SIVH1N1亚型河南株的分离鉴定、HA基因的分离鉴定...... 全文 1-8 , * |
| 展小过等: "表达H3N2亚型猪流感病毒HA基因重组腺病毒对小鼠免疫原性的研究", 《中国农业科学》, vol. 43, no. 6, 31 December 2010 (2010-12-31), pages 1235 - 1241 * |
| 段廷云: "SIVH1N1亚型河南株的分离鉴定、HA基因的分离鉴定……", 《河南农业大学硕士学位论文》, 31 December 2006 (2006-12-31) * |
Cited By (80)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104483360A (en) * | 2014-12-08 | 2015-04-01 | 中国科学院烟台海岸带研究所 | Potential paper chip and preparation and application thereof |
| CN105879932A (en) * | 2015-01-18 | 2016-08-24 | 宁波大学 | Multi-channel micro-fluidic chip device for simultaneously detecting various kinds of subtype swine flu |
| CN105879934A (en) * | 2015-01-18 | 2016-08-24 | 宁波大学 | Multi-channel micro-fluidic chip device for simultaneous detection of swine flu of multiple subtypes |
| CN106153888A (en) * | 2015-03-11 | 2016-11-23 | 宁波大学 | The hypotype swine flue detection device that fluid drives component can remove rapidly |
| CN106153898A (en) * | 2015-03-11 | 2016-11-23 | 宁波大学 | The additional hypotype swine flue detection device driving liquid stream component to be prone to removal |
| CN106153955A (en) * | 2015-03-18 | 2016-11-23 | 宁波大学 | It is prone to the hypotype swine flue detection device applying cheap material disassembled |
| CN106153695A (en) * | 2015-03-31 | 2016-11-23 | 宁波大学 | Comprise the AIDS diagnosis multichannel micro-fluidic chip device of hydrophobic substrate |
| CN106153694A (en) * | 2015-03-31 | 2016-11-23 | 宁波大学 | The hypotype swine flue detection chip apparatus using PDMS to be substrate material |
| CN106153706A (en) * | 2015-03-31 | 2016-11-23 | 宁波大学 | Using hydrophobic material as the AIDS diagnosis device of its substrate of micro-fluidic chip |
| CN106153702A (en) * | 2015-04-23 | 2016-11-23 | 宁波大学 | Coupling Dual Drive detects the micro flow control chip device of multiple hypotype swine flue simultaneously |
| CN106153697A (en) * | 2015-04-23 | 2016-11-23 | 宁波大学 | Multichannel and the cholera diagnosis micro flow control chip device of Dual Drive coupling running |
| CN106153701A (en) * | 2015-04-23 | 2016-11-23 | 宁波大学 | Detect multiple hypotype swine flue Dual Drive CGCM micro flow control chip device |
| CN106290526A (en) * | 2015-05-11 | 2017-01-04 | 宁波大学 | The hypotype swine flue detection device of the Dual Drive CGCM conveniently disassembled |
| CN106290929A (en) * | 2015-05-11 | 2017-01-04 | 宁波大学 | The hypotype swine flue detection device of the Dual Drive CGCM that can disassemble rapidly |
| CN106290931A (en) * | 2015-05-26 | 2017-01-04 | 宁波大学 | The hypotype swine flue detection Multichannel device of substrate is made with cheap hydrophobic material |
| CN106290930A (en) * | 2015-05-26 | 2017-01-04 | 宁波大学 | The hypotype swine flue multi-channel testing device of the Dual Drive coupling containing hydrophobic substrate |
| CN106290935A (en) * | 2015-06-11 | 2017-01-04 | 宁波大学 | A kind of hypotype swine flue detection micro flow control chip device using strong hydrophobic substrate |
| CN106556709A (en) * | 2015-09-24 | 2017-04-05 | 宁波大学 | The micro flow control chip device comprising hydrophobic substrate of coupling Dual Drive pattern |
| CN106556708A (en) * | 2015-09-24 | 2017-04-05 | 宁波大学 | The micro flow control chip device containing hydrophobic substrate of two kinds of type of drive coupling runnings |
| CN106568953A (en) * | 2015-10-12 | 2017-04-19 | 宁波大学 | Microfluidic chip device for detecting multiple tumor markers based on 15-channel joint detection |
| CN106568952A (en) * | 2015-10-12 | 2017-04-19 | 宁波大学 | Micro-fluidic chip device for simultaneously detecting women's various typical tumor markers |
| CN106610399A (en) * | 2015-10-26 | 2017-05-03 | 宁波大学 | Integrated structure using dual-drive coupled tumor marker combined detection chip apparatus |
| CN106610429A (en) * | 2015-10-26 | 2017-05-03 | 宁波大学 | Dual-drive tumor marker joint inspection chip device with PDMS as substrate |
| CN106680348A (en) * | 2015-11-07 | 2017-05-17 | 宁波大学 | Easy-to-assemble chip device for joint detection of male typical tumor markers |
| CN106680349A (en) * | 2015-11-07 | 2017-05-17 | 宁波大学 | Typical tumor marker combined detection chip device for female medical screening |
| CN106814119A (en) * | 2015-11-30 | 2017-06-09 | 宁波大学 | Physical examination examination male's classifiable tumor mark micro flow control chip device |
| CN106990242A (en) * | 2016-01-20 | 2017-07-28 | 李榕生 | The multichannel micro-fluidic chip device of a variety of hypotype swine flus is detected simultaneously |
| CN106984367A (en) * | 2016-01-20 | 2017-07-28 | 李榕生 | Interphase interface characteristic is relied on to reach the micro flow control chip device of sample sap flow process |
| CN106984365A (en) * | 2016-01-20 | 2017-07-28 | 李榕生 | The multichannel micro-fluidic chip device that a variety of hypotype swine flus are detected simultaneously |
| CN107020158A (en) * | 2016-02-01 | 2017-08-08 | 葛宇杰 | The hypotype swine flu detection device that fluid driving can be removed rapidly with component |
| CN107020160A (en) * | 2016-02-01 | 2017-08-08 | 葛宇杰 | Additional driving liquid stream is easy to the hypotype swine flu detection means of removal with component |
| CN107045060A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | Easily-disassembled hypotype swine flu multi-channel testing device comprising electromagnetism auxiliary part |
| CN107042127A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | The cholera diagnosis micro fluidic device of quick removal is capable of in sample liquid driving with component |
| CN107042126A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | Using the micro flow control chip device of PDMS material substrates |
| CN107045011A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | It is a kind of to be not only easily installed but also be easy to the cholera diagnosis Multichannel device disassembled |
| CN107045059A (en) * | 2016-02-05 | 2017-08-15 | 李榕生 | It is easy to the hypotype swine flu detection device for applying cheap material disassembled |
| CN107081179A (en) * | 2016-02-15 | 2017-08-22 | 葛宇杰 | The micro flow control chip device of substrate material is used as using dimethyl silicone polymer |
| CN107085101A (en) * | 2016-02-15 | 2017-08-22 | 葛宇杰 | Strong hydrophobic PDMS as substrate material hypotype swine flu detection chip apparatus |
| CN107085016A (en) * | 2016-02-15 | 2017-08-22 | 葛宇杰 | PDMS is used for the hypotype swine flu detection chip apparatus of substrate material |
| CN107115900A (en) * | 2016-02-25 | 2017-09-01 | 葛宇杰 | For the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter |
| CN107115904A (en) * | 2016-02-25 | 2017-09-01 | 葛宇杰 | Coupling dual drive detects the micro flow control chip device of a variety of hypotype swine flus simultaneously |
| CN107115903A (en) * | 2016-02-25 | 2017-09-01 | 葛宇杰 | Detect a variety of hypotype swine flu dual drive CGCM micro flow control chip devices |
| CN107138185A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | The controller used in syphilis diagnosis micro fluidic device of the dual drive CGCM of easily-disassembled framework |
| CN107138184A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | Driving is with the easily-disassembled and recyclable cholera diagnosis device of component |
| CN107138191A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | The hypotype swine flu detection device for the dual drive CGCM that can be disassembled rapidly |
| CN107138192A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | A kind of easily-disassembled cholera diagnosis micro fluidic device of dual drive CGCM |
| CN107138188A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | The hypotype swine flu detection means for the dual drive CGCM conveniently disassembled |
| CN107167505A (en) * | 2016-03-08 | 2017-09-15 | 葛宇杰 | The hypotype swine flu multi-channel testing device of dual drive coupling containing hydrophobic substrate |
| CN107167599A (en) * | 2016-03-08 | 2017-09-15 | 葛宇杰 | Make the hypotype swine flu detection Multichannel device of substrate with cheap hydrophobic material |
| CN107159322A (en) * | 2016-03-08 | 2017-09-15 | 葛宇杰 | The micro flow control chip device of the substrate containing PDMS based on dual drive CGCM |
| CN107185603A (en) * | 2016-03-15 | 2017-09-22 | 李榕生 | Multichannel micro-fluidic device is used in a kind of strong hydrophobic cholera diagnosis of substrate of its chip |
| CN107185606A (en) * | 2016-03-15 | 2017-09-22 | 李榕生 | A kind of hypotype swine flu detection micro flow control chip device using strong hydrophobic substrate |
| CN107185609A (en) * | 2016-03-15 | 2017-09-22 | 李榕生 | A kind of hypotype swine flu detection micro flow control chip device comprising hydrophobic substrate |
| CN107185607A (en) * | 2016-03-15 | 2017-09-22 | 李榕生 | The micro flow control chip device containing hydrophobic substrate of two kinds of type of drive coupling runnings |
| CN107185608A (en) * | 2016-03-15 | 2017-09-22 | 李榕生 | A kind of substrate material is PDMS cholera diagnosis micro flow control chip device |
| CN107238705A (en) * | 2016-03-29 | 2017-10-10 | 李榕生 | Tumor markers is generally investigated with ten Five-channel micro flow control chip devices |
| CN107335483A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | The multichannel micro-fluidic chip device of a variety of hypotype swine flus is detected simultaneously |
| CN107335484A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | The firmly modified micro flow control chip device of its transmission pipeline of sample liquid stream |
| CN107340391A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | The multichannel micro-fluidic chip device that a variety of hypotype swine flus detect simultaneously |
| CN107345959A (en) * | 2016-05-05 | 2017-11-14 | 李榕生 | The multichannel micro-fluidic chip device of a variety of hypotype swine flus is detected simultaneously |
| CN107344126A (en) * | 2016-05-05 | 2017-11-14 | 李榕生 | The multichannel micro-fluidic chip device that a variety of hypotype swine flus detect simultaneously |
| CN107344127A (en) * | 2016-05-05 | 2017-11-14 | 李榕生 | Interphase interface characteristic is relied on to reach the micro flow control chip device of sample sap flow process |
| CN107349980A (en) * | 2016-05-10 | 2017-11-17 | 李榕生 | The hypotype swine flu detection device that fluid driving can be removed rapidly with component |
| CN107349981A (en) * | 2016-05-10 | 2017-11-17 | 李榕生 | A kind of micro flow control chip device for taking new fluid type of drive |
| CN107349978A (en) * | 2016-05-10 | 2017-11-17 | 李榕生 | Additional driving liquid stream is easy to the hypotype swine flu detection means of removal with component |
| CN107362842A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | The cholera diagnosis micro fluidic device of quick removal is capable of in sample liquid driving with component |
| CN107362841A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | Easily-disassembled hypotype swine flu multi-channel testing device comprising electromagnetism auxiliary part |
| CN107362837A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | Be easy to disassemble applies the hypotype swine flu detection device of cheap material |
| CN107362840A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | Using the micro flow control chip device of PDMS material substrates |
| CN107377017A (en) * | 2016-05-17 | 2017-11-24 | 李榕生 | Micro flow control chip device using dimethyl silicone polymer as substrate material |
| CN107377015A (en) * | 2016-05-17 | 2017-11-24 | 李榕生 | Use hypotype swine flu detection chip apparatus of the PDMS for substrate material |
| CN107377016A (en) * | 2016-05-17 | 2017-11-24 | 李榕生 | Strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material |
| CN107486267A (en) * | 2016-06-13 | 2017-12-19 | 李榕生 | The multichannel micro-fluidic chip device that a variety of hypotype swine flus detect simultaneously |
| CN107656054A (en) * | 2016-07-26 | 2018-02-02 | 洪小女 | The hypotype swine flu detection device that fluid driving can be removed rapidly with component |
| CN107649219A (en) * | 2016-07-26 | 2018-02-02 | 宋岳 | Easily-disassembled hypotype swine flu multi-channel testing device comprising electromagnetism auxiliary part |
| CN107649203A (en) * | 2016-07-26 | 2018-02-02 | 洪小女 | Additional driving liquid stream is easy to the hypotype swine flu detection means of removal with component |
| CN107656052A (en) * | 2016-07-26 | 2018-02-02 | 宋岳 | Be easy to disassemble applies the hypotype swine flu detection device of cheap material |
| CN107649208A (en) * | 2016-07-26 | 2018-02-02 | 宋岳 | Strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material |
| CN107649194A (en) * | 2016-07-26 | 2018-02-02 | 宋岳 | Use hypotype swine flu detection chip apparatus of the PDMS for substrate material |
| CN110082527A (en) * | 2019-04-30 | 2019-08-02 | 杭州艾替捷英科技有限公司 | African swine fever virus chemical luminescence reagent kit |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102478582A (en) | Multi-channel micro-fluidic chip for simultaneously detecting various subtype swine influenza viruses | |
| CN101661038B (en) | Special multi-channel micro-flow controller using low-priced conducting material for syphilis diagnosis | |
| JP6013519B2 (en) | Microfluidic device based on integrated electrochemical immunoassay and its substrate | |
| CN101620227B (en) | Multi-channel chip for cholera diagnosis based on structural conductive macromolecular material technology | |
| CN101620228B (en) | Special AIDS diagnosis device combining and utilizing high polymer anoxybiotic pyrolysis product | |
| Farshchi et al. | Microfluidic biosensing of circulating tumor cells (CTCs): Recent progress and challenges in efficient diagnosis of cancer | |
| Henares et al. | Current development in microfluidic immunosensing chip | |
| US8614056B2 (en) | Microfluidic method for measurement or detection involving cells or biomolecules | |
| CN101587123A (en) | Special micro-fluidic chip for cholera diagnosis with one-dimensional self-assembly magnetic bead chain electrodes | |
| CN101581725A (en) | Multichannel micro-fluidic chip specially used for AIDS diagnosis and comprising quasi-one-dimensional sensitive electrodes | |
| CN101587124A (en) | Micro-fluidic chip special for diagnosing syphilis by aid of organic conductor material technology | |
| CN202066861U (en) | Special microfluidic chip used for diagnosing lung cancer rapidly | |
| CN101581724A (en) | Special multichannel syphilis diagnosis device combined with quasi-one-dimensional specific antigen modified electrodes | |
| CN101625359A (en) | Syphilis-diagnosis special multichannel micro-fluidic chip based on electrode modification technology | |
| US9079179B2 (en) | Microfluidic device comprising sensor | |
| Fu et al. | Microfabricated Renewable Beads-Trapping/Releasing Flow Cell for Rapid Antigen− Antibody Reaction in Chemiluminescent Immunoassay | |
| CN202083692U (en) | Microfluidic chip specially used for gastric cancer diagnosis | |
| JP2014530355A5 (en) | ||
| Yoo et al. | Bead packing and release using flexible polydimethylsiloxane membrane for semi‐continuous biosensing | |
| CN202066860U (en) | Pancreatic cancer diagnostic device with composite nano-particle modified electrode | |
| JP2011145309A (en) | Channel reaction method and channel reactor | |
| Xie | Di Chen and Shujing Lin, Microfluidic Electrochemical Detection Techniques of Cancer Biomarkers | |
| CN106814184A (en) | The tumor markers joint-detection six-channel microfluidic chip device simplified | |
| Dey | Microfluidics for early-stage cancer detection | |
| Caetano et al. | Microfluidic Devices with Electrochemical Detection Towards Covid-19 Detection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120530 |