CN101587124A - Micro-fluidic chip special for diagnosing syphilis by aid of organic conductor material technology - Google Patents
Micro-fluidic chip special for diagnosing syphilis by aid of organic conductor material technology Download PDFInfo
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Abstract
The invention relates to a micro-fluidic chip special for diagnosing syphilis by aid of organic conductor material technology, belonging to the field of analytical testing. One of the improved purposes of the related therapy is to rapidly and cheaply diagnose the syphilis; the invention provides a micro-fluidic chip aiming at the purpose. The technical proposal is that: the chip comprises four branch pipes which are mutually connected in parallel; four fibrous working electrodes are installed in the four branch pipes; the fibrous working electrodes are composed by fibrous electrodes and golden coating sensitive films adhered on the fibrous electrodes and embedded with syphilis-specific antigen; each golden coating sensitive film at surface of the four fibrous working electrodes is respectively embedded with four different syphilis-specific antigen substances; the four antigen substances are respectively syphilis-specific antigen TP0684, TP0453, TP0821 and TP0319. The body materials of the fibrous working electrodes are pyrolysis conductive macromolecule materials. The chip in the invention has low cost and multiple channels.
Description
Technical field
The present invention relates to a kind of controller used in syphilis diagnosis micro-fluidic chip special, belong to the analytical test field by means of the organic conductor material technology.
Background technology
Syphilis (Syphilis) be by microspironema pallidum (T.pallidum, a kind of sexually transmitted disease that TP) causes (Sexually transmitted disease, STD).Microspironema pallidum only infects the mankind under natural situation, and the people is the unique infection sources of syphilis, main trafficability characteristic contact transmission.Also can pass through the placenta vertical transmission in addition, cause fetal congenital syphilis.A few peoples also can infect through blood transfusion.Since the eighties, syphilis is revivable once more in China, according to national venereal disease control center statistics, China's syphilis incidence of disease annual growth reaches 52.7%, and the congenital syphilis and the neurolues incidence of disease increase obviously, the trend that rises is gradually also arranged in the whole world, syphilis not only become international serious society and public health problem, and be one of principal disease of harm population health.Therefore, select simple, fast, hypersensitivity and specificity diagnostic method and reagent, to the syphilis early diagnosis, find the infection sources, control syphilis is propagated significant.
Controller used in syphilis diagnosis method commonly used at present has etiological examination, non-syphilitic leptospira antigen serum test, the test of microspironema pallidum hemagglutination (TPHA), polymerase chain reaction (PCR) and TP quick detection test paper bar etc.The principle of etiological examination is to get the exudate of patient's infringement or the tissue fluid that lymphnode puncture obtains, observe spirochetal characteristic morphologic and mode of motion, in theory early syphilis there is higher recall rate, but the restriction (comprising prescription on individual diagnosis medication, high-quality fluorescent microscope, reagent and technology etc.) that is subjected to condition is also more, and therefore actual recall rate is not high.The principle of non-syphilitic leptospira antigen serum test is the serology primary dcreening operation test commonly used of current diagnosis syphilis, though in a lot of documents, all mention non-syphilitic leptospira antigen serum test simple and easy, quick, be easy to observations, the screening serum that is suitable for a large amount of crowds, but susceptibility, specificity are relatively poor, biologic fale-positive and false negative reaction appear sometimes, cause omission and flase drop easily, so be not suitable for the diagnosis of primary syphilis, congenital syphilis, neurolues.Though its susceptibility of TPHA and specificity height mainly are used to the syphilis confirmed diagnosis test, and observation of curative effect is had little significance.Though TP quick detection test paper bar has overcome defectives such as length detection time, reagent costliness, domestic temporarily do not have its sensitivity of play-by-play and specificity effect, so be not useable for confirmed diagnosis test.The PCR method is newer diagnostic method, and it can carry out early stage antidiastole to genital ulcer, distinguishes syphilis, genital herpes and chancroid.But the PCR method of current diagnosis syphilis still exist primer non-specific, to shortcomings such as susceptibility such as serum/whole blood are low.And when the skin damage begins healing, secretion minimizing, serological test positive reaction, adopt PCR method just improper.In addition, PCR method is subject to materials such as tissue in the sample and cell fragment and suppresses, and causes the PCR false negative result, so detect should not be as declaring more for PCR.
Detection methods such as non-syphilitic leptospira antigen serum test, TPHA and PCR and TP quick detection test paper bar all belong to immunoassay.Above immunoassay itself has very strong selectivity, to the early diagnosis of infectious disease, judges that all there is important meaning aspects such as curative effect and prognosis.Yet the detection of serious infectious diseases is very complicated, and traditional immunoassay only detects at monospecific antibody usually.Because a kind of diagnosis antibody can appear at different pathogenic bacteria, or different diagnosis antibody can occur simultaneously in same pathogenic bacteria, therefore, carry out multiple efficiently immune analysis method (SMIAs), the kind and the quantity of, quantitative response various pathogenic bacteria antibody qualitative truly from the angle of biological correlativity, in early days prevention and the multiple infectious disease of joint inspection, judge that aspects such as disease carrying germ development degree, O﹠A result of treatment all have important medical value.
Electrochemical immunosensor has unique advantages such as automaticity height, cheapness, easily preparation, rapid sensitive, cooperates immunoreactive efficiently single-mindedly, is fit to very much the analyser of the multiple immunoassay detection of structure serious infectious diseases.The micro-fluidic chip (lab-on-chip) that the title of " life science integrated circuit " is arranged is the desirable technique that makes up microminiaturized, integrated electrochemical immunosensor.The micro-fluidic chip technology be with sampling, pre-service, add reagent, reaction, separation, detection etc. be integrated in finish on the microchip one in front of the door along technology, have analysis speed fast, contain much information, reagent consumption less, advantage such as pollution is little, instrument is easy to use.And be fit to very much suitability for industrialized production.On behalf of the 21 century analytical instrument, microflow control technique move towards microminiaturized, integrated developing direction.The micro-fluidic Amperometric Detection Coupled chip that the serious infectious diseases that are suitable for structure detect has following advantage: (1) is easy to integrated a plurality of sense channels and detecting electrode on chip by micro-processing technology, accomplishes that multiple target objects analyzes the technical feasibility height simultaneously.(2) by the micro-flowing injection technology, sample flows in pipeline and has guaranteed that electrode surface upgrades constantly, and it is easily contaminated better to have overcome electrode, causes false positive rate height, problem such as collimation difference as a result.(3) microfluidic sensing device upper pipeline diameter has only μ m level, and the reaction tank volume also has only μ L level, and required reagent dosage is few during analysis, and analyte arrives the diffusion length of electrode surface lacks, therefore can significantly reduce the incubation time, realizes the fast joint inspection target.(4) the microfluidic sensing device is many based on the optical detection system at present, and the expensive volume of instrument is big, fully integrated being difficult to, and also it is very high to detect cost, has limited its popularization.And adopting Electrochemical Detection in the sensor technology, volume is little, automaticity is high.
At present, in the micro-fluidic chip technical field, utilize that the multiple specific antigen of syphilis detects simultaneously, the correlation technique and the method for the multiple syphilis antibody of quick diagnosis are not appeared in the newspapers as yet; On the other hand, the gold of costliness that adopt are used as the testing sensor core parts stock of working electrode just more in this field prior art, the use of this expensive metal material, increased the manufacturing cost of related chip, the known danger of detecting in view of chip that relevant infectious disease had, there is unsafe factor in the relevant disease detecting with the gold recovery operation in the micro-fluidic chip, relevant unsafe factor can increase the difficulty of gold recovery operation, increase the cost of gold reclaimer operation, and then may be because of the actual consideration of gain and loss balance aspect economically, cause the loss of precious resource gold, obviously, this technological deficiency also remains to be eliminated.
Summary of the invention
Technical matters to be solved by this invention is, in the so total technological frame of micro-fluidic chip technology, develop a kind of cheapness can utilize the multiple specific antigen of syphilis come to multiple syphilis antibody detect simultaneously, the micro-fluidic chip special and the detection method thereof of quick diagnosis.
The present invention solve the technical problem by following scheme, the device that this scheme provides is a kind of controller used in syphilis diagnosis micro-fluidic chip special by means of the organic conductor material technology, the structure of this micro-fluidic chip comprises that applying is installed in two plate objects together, described two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip, on the position of the mutual applying between described two plate objects, be equiped with pipeline, and, three pond shape things, one end of pipeline via the manifold shape fluid passage respectively with wherein two pond shape thing UNICOMs, the other end of pipeline and a remaining pond shape thing UNICOM, and, be installed in the described pipeline working electrode on the diverse location in regular turn respectively and to electrode and contrast electrode, described working electrode by conductive electrode and be attached to embedding on the described conductive electrode gold size sensitive membrane of syphilis specific antigen constitute, this case special feature is, the structure of described pipeline is structure in parallel, the described pipeline that is structure in parallel is made of four lateral parallel connections, and, the quantity of described working electrode is four, the installation position of these four working electrodes lays respectively in described four laterals, and, specific antigen material in its top layer gold size sensitive membrane structure of these four working electrodes is respectively four kinds of syphilis antigenic substances to the combination of syphilis antibody energy specificity, these four kinds of antigenic substances are respectively syphilis specific antigen TP0684, TP0453, TP0821 and TP0319, and, its pattern of each working electrode all is fibrous, and it is thermal decomposition conducting polymer material that described pattern is its body material of fibrous working electrode.
Described pipeline in the described microfluidic chip structure and described lateral and described manifold shape fluid passage, its internal diameter size all can be selected arbitrarily size, but, for as far as possible less with the consideration of aspects such as liquid sample to be measured and reduction reagent loss, described pipeline and described lateral and described manifold shape fluid passage are preferably all selected the passage of capillary level for use, the passage meaning of described capillary level is a capillary channel, and the internal diameter capillaceous on its internal diameter and the ordinary meaning is suitable.The shape of cross section of its inner passage of described kapillary can be a shape arbitrarily, described shape of cross section is for example circular, oval, square, rectangle, bar shaped, can certainly be have bending arbitrarily linear, and, described interior shape capillaceous is along with the extension of pipeline, and it is different shapes that the shape of cross section of different parts also can allow.Only with regard to kapillary one speech, its art-recognized meanings is known.
Described gold size sensitive membrane is that shitosan gold size solution and syphilis specific antigenic solution are fully mixed, and is sprayed on the structure position of setting, and its drying and forming-film is formed.Described gold size sensitive membrane has been included as fixing above-mentioned each syphilis specific antigen and has introduced wherein complementary medium, and described complementary medium is wherein a kind of of shitosan, cellulose acetate, gelatin or their potpourri for example.
What relate in the structure is minute sized electrode to electrode and contrast electrode, and its electrode shape all can be selected arbitrarily shape, and described selected arbitrarily shape is square shape sheet shape, rectangle sheet, strip or round sheet or the like for example.
Working electrode in this case chip is fibrous, the fibrous pattern of electrode shows that it can with the pipeline of described pipeline especially capillary level or to say minim channel adaptive well, based on this, this pattern working electrode can directly be placed in the position in the pipeline, and this design feature also helps the forced working electrode and the contacting between its peripheral measured object liquid stream of flowing through.
Relate to several pond shape things in this case microfluidic chip structure, described pond shape thing is pond shape or the scrotiform structure that is used for transitional liquid storage, its shape of inner chamber of each pond shape thing wherein all can be selected arbitrarily shape, and described cavity shape is cylindrical cavity shape, square column type cavity-like, oblong cavity shape or spherical hollow space shape or the like for example.The size of described pond shape thing can be arbitrarily selected size, still, for can be as far as possible less with liquid sample to be measured and reduce reagent loss, the hypomegetic pond shape thing that described pond shape thing preferably can mate with kapillary.
This case device can further include some annexes certainly, and described annex is multiple tracks electrochemical workstation and microfluidic pump or the like for example, and the art-recognized meanings of the art-recognized meanings of described multiple tracks electrochemical workstation and microfluidic pump is known.Each working electrode that relates in this case microfluidic chip structure and to electrode and contrast electrode etc., can get lines crossed via corresponding special use is respectively connecting with the corresponding interface of described multiple tracks electrochemical workstation.It is to be used for the private cable that each the corresponding interface with each described electrode and described multiple tracks electrochemical workstation is coupled to each other that described special use is being got lines crossed.Described microfluidic pump is exclusively used in and drives micro liquid and flow, described microfluidic pump can with the described pond of selected any one shape thing UNICOM as required.
The organism that general its formation element is carbon, hydrogen, oxygen, nitrogen can both form the polymer substance with conductive capability greatly through after the high-temperature heat treatment, but, take all factors into consideration many-sided factor, recommendation or to say preferred described thermal decomposition conducting polymer be the conductive material that is after heat treatment formed by polyimide or polyacrylonitrile.
Can from existing scientific and technical literature, seek about the technology of preparing of thermal decomposition conducting polymer.
The conductive material that is after heat treatment formed by polyimide or polyacrylonitrile is called the structural conductive macromolecular material again, this material has the structure that is similar to graphite, electric conductivity is good, and, this conductive material stable in properties, the chemistry performance is inertia relatively, chemical property with the comparatively inertia that is similar to the element gold, be the described fibrous working electrode of body material so the quality that can compare favourably with the working electrode that with the gold is the body material has also just been arranged with this material, and, comparatively speaking, cheap significantly, its inner crucial test member adopts the described chip of this cheap high-performance material so cost to decrease.Described inertia refers between electrode body material and the electrode sensitive entry material and the possible exposed part that is not covered fully by the electrode sensitive entry material of electrode body is difficult for causing the chemical action that goes bad when treating to contact between the sample measuring liquid.
It is the suitable filament diameter of setting arbitrarily of being convenient to install and use that the filament diameter of described fibrous working electrode can allow, still, recommendation or say preferred described its scope of filament diameter between 0.1 micron to 2000 microns; It is the filament length of setting arbitrarily of being convenient to install and use that the filament length of described fibrous working electrode can allow, still, recommendation or to say preferred described its scope of filament length be between 1 micron to 150000 microns.
Be installed in the described gold size sensitive membrane of the filament superficial layer of described fibrous working electrode by spraying or the coating of point sample instrument point sample or other appropriate process, it is the thickness of any sample measuring liquid treated generation electrical signals response of setting that its thicknesses of layers can allow, but the in other words conj.or perhaps preferred thickness of the thickness of recommendation is between 10 nanometers and 200 nanometers.
It is any electrical insulating property material that described cover plate and substrate in the chip structure, its material can allow, for example: and polypropylene, glass, or the like, preferred material is a polymethylmethacrylate.
It is the width value that can reach any setting of dependence test purpose that described cover plate in the structure and its width of substrate can allow, and still, preferred described width value is all between 2 centimetres and 4 centimetres; It is the length value of setting arbitrarily that can reach the dependence test purpose that the length value of described cover plate and substrate can allow too, and still, preferred length value or the length value of saying recommendation are between 3 centimetres and 6 centimetres.
It is the thickness of setting arbitrarily of being convenient to the device assembling that its thickness of described cover plate and substrate can allow, the thickness of recommendation or to say preferred thickness be between 0.5 millimeter and 5 millimeters.Less thickness helps saving material.
Described fibrous working electrode both can be the working electrode that its filament is the vertical bar form, described working electrode can certainly be the working electrode that its filament is bending shape, even, described working electrode can also be its filament working electrode of form in the shape of a spiral; That recommend or to say preferred working electrode be its filament working electrode of form in the shape of a spiral, that is to say, preferred described fibrous working electrode is that its fibrous electrodes body disc revolves the working electrode of spread spirality attitude, and its pattern of the working electrode of this spiral is similar to spiral spring.The working electrode of described spiral helps effective contact interface of expansion electrode and determinand liquid stream significantly, improve detection sensitivity, and, the working electrode of spiral is because of its mechanical pattern, naturally possessed certain distortion adaptive faculty under external force, this design feature makes the working electrode of described spiral more can tolerate the fluid impact effect from determinand liquid stream accidental or pulsation, and is unlikely to rupture easily, damage.
The using method of this case micro-fluidic chip:
Employing adds Micropump and drives the steady flow in the capillary channel of four-way micro-fluidic chip of liquid stream, utilizes the four-way electrochemical analytical instrument respectively four kinds of controller used in syphilis diagnosis antibody to be detected.
The concrete detection of this case micro-fluidic chip uses step as follows:
1, add blood serum sample liquid in the microtubule road, adding under the Micropump driving, various syphilis antibody molecules are by the corresponding syphilis specific antigen capture of gold size sensitive membrane embedding on the electrode surface in each passage.
2, the ELIAS secondary antibody that adds horseradish peroxidase-labeled forms immune complex by sandwich method desmoenzyme mark " two is anti-".
3, adopt the hyperchannel electroanalyzer, add electron mediators such as catechol, adopt ampere method detection " two is anti-" to go up enzyme and cause H
2O
2The electric current of reduction changes, and obtains the kind and the content of each analyte thus.
4, the result is carried out analysis-by-synthesis, syphilis antibody is carried out comprehensive diagnos.
Be necessary that doing one here seems unnecessary supplementary notes: antibody and antigen be relative to each other but different materials, the mutual relationship between them, metaphor more popularly is like being the lock in the cover lockset and the relation of key.
Advantage of the present invention is, integrated four fibrous working electrodes that are coated with four kinds of syphilis specific antigenic substances respectively on a micro-fluidic chip, these four fibrous working electrodes are respectively at four kinds of feature diagnosis antibody that detect syphilis, this case micro-fluidic chip is a kind of controller used in syphilis diagnosis micro-fluidic chip special that can utilize the multiple specific antigen of syphilis to detect simultaneously, and the design feature of its integrated structure has determined the use of this chip to help to accelerate controller used in syphilis diagnosis speed, improves accuracy rate of diagnosis, reduced the controller used in syphilis diagnosis expense; Its fibrous pattern of working electrode in the chip structure show this kind working electrode be easy to chip in pipeline adaptive mutually; Under the situation of selecting described spiral working electrode for use, its electrode shape also helps the forced working electrode to contact with detected sample liquid stream chemistry each other, and simultaneously, this form electrode further has the mechanical tolerance of the acute impact of anti-current body; It is thermal decomposition conducting polymer material that the described pattern of this case is its body material of fibrous working electrode, the thermal decomposition conducting polymer composite is cheap, preparation technology is simple, be easy to produce in large quantity, compared to being the preparation technology of the general micro-fluidic chip of electrode material with the gold, this case helps to reduce the chip manufacturing cost significantly.
Description of drawings
Fig. 1 is this case micro-fluidic chip embodiment organigram, what showed is the form of the perspective under the depression angle of this example structure, do not depict described annex among the figure, detailed the describing of yet the CONSTRUCTED SPECIFICATION of described fibrous working electrode not being amplified among the figure.
Fig. 2 is a common spiral spring mentioned in this article, and this figure exhibition is listed in this, in order that conveniently understand the fibrous working electrode of the described spiral of this case, the two likeness in form.
Among the figure, 1,2,10 is respectively three pond shape things that installation position is different, the 3rd, the manifold shape fluid passage, 4,7,11,14 is respectively different but four laterals of formation parallel with one another UNICOM in parallel structure of installation position, the 5th, the specific antigen material that is installed in its top layer gold size sensitive membrane structure in the lateral 4 is the fibrous working electrode of syphilis specific antigen TP0684, the 6th, the specific antigen material that is installed in its top layer gold size sensitive membrane structure in the lateral 7 is the fibrous working electrode of syphilis specific antigen TP0453, the 12nd, the specific antigen material that is installed in its top layer gold size sensitive membrane structure in the lateral 11 is the fibrous working electrode of syphilis specific antigen TP0821, the 13rd, the specific antigen material that is installed in its top layer gold size sensitive membrane structure in the lateral 14 is the fibrous working electrode of syphilis specific antigen TP0319, the 8th, to electrode, the 9th, contrast electrode.
Embodiment
In this case embodiment that Fig. 1 showed, the structure of this micro-fluidic chip comprises that applying is installed in two plate objects together, two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip, on the position of the mutual applying between two plate objects, be equiped with pipeline, and, three pond shape things, these three pond shape things are respectively pond shape things 1, pond shape thing 2 and pond shape thing 10, one end of described pipeline via manifold shape fluid passage 3 respectively with pond shape thing 1 and pond shape thing 2 UNICOMs, the other end of described pipeline and remaining pond shape thing 10 UNICOMs, and, be installed in the described pipeline working electrode on the diverse location in regular turn respectively and to electrode 8 and contrast electrode 9, working electrode by conductive electrode and be attached to embedding on the described conductive electrode gold size sensitive membrane of syphilis specific antigen constitute, the structure of described pipeline is structure in parallel, this pipeline that is structure in parallel is made of four lateral parallel connections, these four laterals are respectively lateral 4 and lateral 7 and lateral 11 and lateral 14, and, the quantity of fibrous working electrode is four, these four fibrous working electrodes are respectively fibrous working electrode 5 and fibrous working electrode 6 and fibrous working electrode 12 and fibrous working electrode 13, wherein, fibrous working electrode 5 is that the specific antigen material in its top layer gold size sensitive membrane structure that is installed in the lateral 4 is the fibrous working electrode of syphilis specific antigen TP0684, fibrous working electrode 6 is that the specific antigen material in its top layer gold size sensitive membrane structure that is installed in the lateral 7 is the fibrous working electrode of syphilis specific antigen TP0453, fibrous working electrode 12 is that the specific antigen material in its top layer gold size sensitive membrane structure that is installed in the lateral 11 is the fibrous working electrode of syphilis specific antigen TP0821, fibrous working electrode 13 is that the specific antigen material in its top layer gold size sensitive membrane structure that is installed in the lateral 14 is the fibrous working electrode of syphilis specific antigen TP0319, and the body material of fibrous working electrode 5 and fibrous working electrode 6 and fibrous working electrode 12 and fibrous working electrode 13 all is thermal decomposition conducting polymer materials.
Do not draw among Fig. 1 as associate members such as the microfluidic pump of annex and multiple tracks electrochemical workstations, do not have the CONSTRUCTED SPECIFICATION of the fibrous working electrode of detailed displaying yet.
Each pond shape thing in this routine structure can be as required with as the microfluidic pump of annex UNICOM by any means.
Each working electrode in this routine structure and to electrode and contrast electrode can be respectively via special-purpose separately cable or say get lines crossed respectively with as the corresponding cable interface of the multiple tracks electrochemical workstation of annex or say the interface of getting lines crossed and connect.
Claims (9)
1. by means of the controller used in syphilis diagnosis micro-fluidic chip special of organic conductor material technology, the structure of this micro-fluidic chip comprises that applying is installed in two plate objects together, described two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip, on the position of the mutual applying between described two plate objects, be equiped with pipeline, and, three pond shape things, one end of pipeline via the manifold shape fluid passage respectively with wherein two pond shape thing UNICOMs, the other end of pipeline and a remaining pond shape thing UNICOM, and, be installed in the described pipeline working electrode on the diverse location in regular turn respectively and to electrode and contrast electrode, described working electrode by conductive electrode and be attached to embedding on the described conductive electrode gold size sensitive membrane of syphilis specific antigen constitute, it is characterized in that, the structure of described pipeline is structure in parallel, the described pipeline that is structure in parallel is made of four lateral parallel connections, and, the quantity of described working electrode is four, the installation position of these four working electrodes lays respectively in described four laterals, and, specific antigen material in its top layer gold size sensitive membrane structure of these four working electrodes is respectively four kinds of syphilis antigenic substances to the combination of syphilis antibody energy specificity, these four kinds of antigenic substances are respectively syphilis specific antigen TP0684, TP0453, TP0821 and TP0319, and, its pattern of each working electrode all is fibrous, and it is thermal decomposition conducting polymer material that described pattern is its body material of fibrous working electrode.
2. the controller used in syphilis diagnosis micro-fluidic chip special by means of the organic conductor material technology according to claim 1 is characterized in that, described pipeline and described lateral and described manifold shape fluid passage are capillary channel.
3. the controller used in syphilis diagnosis micro-fluidic chip special by means of the organic conductor material technology according to claim 1 is characterized in that described thermal decomposition conducting polymer is the conductive material that is after heat treatment formed by polyimide or polyacrylonitrile.
4. the controller used in syphilis diagnosis micro-fluidic chip special by means of the organic conductor material technology according to claim 3, it is characterized in that, the filament diameter of described fibrous working electrode is between 0.1 micron to 2000 microns, and the filament length of described fibrous working electrode is between 1 micron to 150000 microns.
5. the controller used in syphilis diagnosis micro-fluidic chip special by means of the organic conductor material technology according to claim 1 is characterized in that the thickness of described gold size sensitive membrane is between 10 nanometers and 200 nanometers.
6. the controller used in syphilis diagnosis micro-fluidic chip special by means of the organic conductor material technology according to claim 1 is characterized in that, the described cover plate in the structure and its material of substrate are polymethylmethacrylate.
7. the controller used in syphilis diagnosis micro-fluidic chip special by means of the organic conductor material technology according to claim 1, it is characterized in that, all between 2 centimetres and 4 centimetres, its length is all between 3 centimetres and 6 centimetres for described cover plate in the structure and its width of substrate.
8. the controller used in syphilis diagnosis micro-fluidic chip special by means of the organic conductor material technology according to claim 1 is characterized in that, the described cover plate in the structure and its thickness of substrate are all between 0.5 millimeter and 5 millimeters.
9. the controller used in syphilis diagnosis micro-fluidic chip special by means of the organic conductor material technology according to claim 1, it is characterized in that, described fibrous working electrode is that its fibrous electrodes body disc revolves the working electrode of spread spirality attitude, and its pattern of the working electrode of this spiral is similar to spiral spring.
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| CN107413396A (en) * | 2016-05-23 | 2017-12-01 | 李榕生 | The classifiable tumor markers in detecting micro flow control chip device that male is applicable |
| CN107413400A (en) * | 2016-05-23 | 2017-12-01 | 李榕生 | The classifiable tumor markers in detecting micro flow control chip device that women is applicable |
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| CN107649201A (en) * | 2016-07-26 | 2018-02-02 | 宋岳 | The controller used in syphilis diagnosis device that sample driving can be removed fast with function element |
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