CN101587123A - Special micro-fluidic chip for cholera diagnosis with one-dimensional self-assembly magnetic bead chain electrodes - Google Patents
Special micro-fluidic chip for cholera diagnosis with one-dimensional self-assembly magnetic bead chain electrodes Download PDFInfo
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- CN101587123A CN101587123A CNA2009101509304A CN200910150930A CN101587123A CN 101587123 A CN101587123 A CN 101587123A CN A2009101509304 A CNA2009101509304 A CN A2009101509304A CN 200910150930 A CN200910150930 A CN 200910150930A CN 101587123 A CN101587123 A CN 101587123A
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Abstract
The invention relates to a special micro-fluidic chip for cholera diagnosis with one-dimensional self-assembly magnetic bead chain electrodes, belonging to the field of testing. Rapid diagnosis of fulminating infectious disease cholera with low cost is one of many expectations in the development of medical technology, and the invention provides a micro-fluidic chip for simultaneously detecting and rapidly diagnosing cholera by using multiple characteristic antibodies of cholera. The chip is provided with three micro liquid storage tanks, and the invention is characterized in that capillary passages in parallel configuration contain in the chip, the parallel configuration contains four micro-passages that are mutually in parallel, four magnetic bead chain working electrodes are respectively installed inside the four micro-passages, the surface substances of the four magnetic bead chain working electrodes are respectively four antibody substances that are cholera TP0821 antibody, cholera TP0319 antibody, cholera TP0624 antibody and cholera O139 mycoprotein antibody. The integrated structure and the appearances of specific magnetic bead chain working electrodes facilitate to improve the diagnosis efficiency of cholera diseases.
Description
Technical field
The present invention relates to a kind of special micro-fluidic chip for cholera diagnosis that has one-dimensional self-assembly magnetic bead chain electrodes, belong to field tests.
Background technology
Cholera is a kind of deadly infectious disease that is caused by comma bacillus, belongs to the international quarantine infectious disease, also is one of category A infectious disease cause of disease of China's law on the prevention and control of infectious diseases regulation.This disease morbidity is anxious, propagation is fast, coverage is wide, can cause that part and global eruption and prevalence are feature; It is the infectious disease of a kind of serious harm human health and life.Comma bacillus is to cause the cacatory pathogen of the infected, as the international quarantine infectious disease---the etiological diagnosis of cholera, to detect the O1 group cholera vibrio or the O139 group cholera vibrio is as the criterion.
For a long time, all be to adopt traditional method of inspection to the diagnosis of comma bacillus, i.e. morphology, physiological and biochemical property and serological Identification and bacteriophage typing etc., the fastest also wanted just can finish in tens hours.Time is long, sample size is big, accuracy rate is low, and once can only obtain a kind of pathogenic bacteria kind and quantity.In recent years, on traditional detection method basis, monoclonal antibody and Protocols in Molecular Biology are applied in the research of comma bacillus more and more, and the develop rapidly of dependence test method provides new strong means for the detection of comma bacillus.Wherein mainly contain: colloid gold label immunochromatography technique, real-time fluorescence PCR technology, gene sensor technology, IiT, enzyme linked immunological adsorption technology and nucleic acid sequence analysis technology etc.Yet in the said method, the separation of pathogen is wasted time and energy, inapplicable rapid batch diagnosis.Because need hatch, multiple steps such as wash-out, colour developing, generally need a few hours.Nucleic acid PCR detects Zhu Fazhong, and real-time fluorescence quantitative PCR susceptibility height, high specificity can shorten the detection window phase, but need expensive instrument and equipment, can't large tracts of land promote, and isolating nucleic acid and carry out the PCR reaction and also need a few hours, so can't fast detecting.Immunity colloidal gold test paper strip can obtain the result in several minutes, realize fast detecting, but generally can only reach the semiquantitative determination standard, and susceptibility is poor, and the cost height also is difficult to detect simultaneously multiple antigen.Though time-resolved fluorescence immunoassay method and chemiluminescence method that development in recent years is very fast are highly sensitive, still need loaded down with trivial details pre-treatment, and detection costs an arm and a leg.Wherein immuno analytical method is to realize the accurately important means of diagnosis of infectious disease, by measuring 2 all serum antibiotic " diagnosis albumen " (antigen/antibody is called for short DP) content after being ill, can obtain the kind and the quantity of pathogen.Immunoassay itself has very strong selectivity, to the early diagnosis of infectious disease, judges that all there is important meaning aspects such as curative effect and prognosis.Yet the detection of serious infectious diseases bacterium is very complicated, and traditional immunoassay only detects at single index usually.Because a kind of diagnosis albumen can appear at different germs, or different diagnosis albumen can occur simultaneously in same pathogenic bacteria, therefore, carry out multiple efficiently immune analysis method (SMIAs), from the angle of biological correlativity kind and the quantity of the various pathogenic bacteria of quantitative response truly; Aspects such as prevention and the multiple infectious disease of joint inspection, judgement disease carrying germ development degree, O﹠A result of treatment all have important medical value in early days.
O1, the joint inspection of O139 comma bacillus need be obtained TP0821, TP0319, TP0624 and cholera vibrio O 139 mycoprotein, mannose sensitive haemagglutination several diagnosis protein contents such as (rMSHA) simultaneously, analysis-by-synthesis just can obtain, wherein, the detection of preceding four kinds of protein contents has more diagnostic significance.
Described diagnosis albumen TP0821, TP0319, TP0624, cholera vibrio O 139 mycoprotein, and, corresponding TP0821 antibody, TP0319 antibody, TP0624 antibody, O139 mycoprotein antibody, its art-recognized meanings is known in cholera diagnosis and treatment technical field.
The micro-fluidic chip technology be with sampling, pre-service, add carry out on the microchip that reagent, reaction, separation, detection etc. are integrated in more than square centimeters one in front of the door along technology, have analysis speed fast, contain much information, reagent consumption less, advantage such as pollutions is little, operation cost is low, instrument is easy and detection speed and sensitivity is all good.Microflow control technique is being represented the developing direction of 21 century analytical instrument.At present mainly contain uv absorption detection, laser-Induced Fluorescence Detection, electricity and lead detections, volt-ampere detection etc. based on the detection technique of micro-fluidic chip.Wherein uv absorption detect and electricity to lead detection more common, but its sensitivity is lower; Laser-Induced Fluorescence Detection and volt-ampere detection sensitivity are higher, but determination object is few, has limited their popularization.And adopting Electrochemical Detection in the sensor, volume is little, automaticity is high, cost is low, is very suitable for on-the site analysis.
At present, in the micro-fluidic chip technical field, utilize that cholera various features antibody detects simultaneously, the technology of quick diagnosis cholera is not appeared in the newspapers as yet.
Summary of the invention
Technical matters to be solved by this invention is, in the so total technological frame of micro-fluidic chip technology, develop a kind of micro-fluidic chip special that is applied to quick diagnosis deadly infectious disease cholera that can utilize cholera various features antibody to detect simultaneously.
The present invention solve the technical problem by following scheme, the device that this scheme provides is a kind of special micro-fluidic chip for cholera diagnosis that has one-dimensional self-assembly magnetic bead chain electrodes, the structure of this micro-fluidic chip comprises that applying is installed in two plate objects together, described two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip, on the position of the mutual applying between described two plate objects, be equiped with capillary channel, and, three little liquid storage tanks, one end of capillary channel via Y font three UNICOM roads respectively with the little liquid storage tank of wherein two UNICOM, the other end of capillary channel and a remaining little liquid storage tank UNICOM, and, be installed in the described capillary channel working electrode on the diverse location in regular turn respectively and to electrode and contrast electrode, described working electrode is made of conductive electrode and the antibody materials top layer that is coated on the described conductive electrode, this case special feature is, the structure of described capillary channel is structure in parallel, the described capillary channel that is structure in parallel is made of four minim channel parallel connections, and, the quantity of described working electrode is four, the installation position of these four working electrodes lays respectively in described four minim channels, and, antibody materials in these four its surface structures of working electrode is respectively four kinds of antibody materials, these four kinds of antibody materials are respectively cholera TP0821 antibody and cholera TP0319 antibody and cholera TP0624 antibody and cholera O139 mycoprotein antibody, and its pattern of each working electrode all is beading.Described antibody materials is included as fixing described each cholera antibody and introduces wherein complementary medium, described complementary medium for example sulfydryl butanediamine copper is modified medium, described conductive electrode can at first utilize described complementary medium to carry out finishing and handle, coat the antibody materials top layer afterwards thereon, antibody materials top layer and described complementary medium one integrated mass.
What relate in the structure is minute sized electrode to electrode and contrast electrode, and its electrode shape all can be selected arbitrarily shape, and described selected arbitrarily shape is square shape sheet shape, rectangle sheet, strip or round sheet or the like for example.
Working electrode in this case chip is beading, its beading electrode profile helps to expand significantly its effective working interface, and, further, the beading pattern also makes this working electrode have good flow-disturbing, and this helps the forced working electrode and the contacting between its peripheral measured object liquid stream of flowing through.
Relate to several little liquid storage tanks in this case microfluidic chip structure, described little liquid storage tank is little pond or the micro-capsule that is used for transitional liquid storage, its shape of miniature inner chamber of the little liquid storage tank of wherein each all can be arbitrarily selected shape, for example miniature cylindrical cavity shape of described cavity shape, miniature square column type cavity-like, miniature oblong cavity shape or miniature spherical hollow space shape or the like.This case device can further include some annexes certainly, and described annex is multiple tracks electrochemical workstation and microfluidic pump or the like for example, and the art-recognized meanings of the art-recognized meanings of described multiple tracks electrochemical workstation and microfluidic pump is known.Each working electrode that relates in this case microfluidic chip structure and to electrode and contrast electrode etc., can get lines crossed via corresponding special use is respectively connecting with the corresponding interface of described multiple tracks electrochemical workstation.It is to be used for the private cable that each the corresponding interface with each described electrode and described multiple tracks electrochemical workstation is coupled to each other that described special use is being got lines crossed.Described microfluidic pump is used to drive micro liquid and flows, described microfluidic pump can with the described little liquid storage tank of selected any one as required UNICOM.
Described pattern is catenate working electrode, both can be the beading conductive electrode of integrated compression moulding, also can be the beading conductive electrode of moulding by casting; It is the magnetic bead string structure of electric conductivity that its structure of described working electrode can certainly allow, under this situation, it is the tri-iron tetroxide material with conductive capability that described pattern is its material of catenate working electrode, and it is to add the accurate one-dimensional electric electrode that is spliced mutually under the magnetic field condition guiding by the magnetic bead of many tri-iron tetroxide materials that described pattern is catenate working electrode.
Described pattern is catenate working electrode, it is the working electrode of described magnetic bead string structure, magnetic bead diameter in its structure is not limit, the diameter of described magnetic bead can be the size of setting as required arbitrarily, but, the preferred diameter range of described magnetic bead is between 20 nanometer to 2000 nanometers, and, it is the number of setting as required arbitrarily that the number of the magnetic bead that is stitched together mutually that is contained in the structure of each described working electrode can allow, described number is not limit, but the preferred value of the number of the magnetic bead of a described working electrode of formation is between 100 to 100000.
In view of each described beading working electrode occupation space width less, this working electrode can allow directly to be placed in the capillary channel, narrow relatively fluid passage more helps representing the disturbance ability to peripheral working fluid of beading electrode, and, also help the potentiality of its effective working interface given full play of.
Can described four kinds of cholera antibody materials be coated with the surface that is installed in described four beading conductive electrodes respectively by spraying or point sample instrument point sample or other appropriate process, form described antibody materials top layer.It is the thickness of any sample measuring liquid treated generation electrical signals response of setting that the thickness on described antibody materials top layer can allow, and still, the in other words conj.or perhaps preferred thickness of the thickness of recommendation is between 2 nanometers and 100 nanometers.
It is any electrical insulating property material that described cover plate and substrate in the chip structure, its material can allow, for example: and polypropylene, glass, or the like, preferred material is a polymethylmethacrylate.
It is the width value that can reach any setting of dependence test purpose that described cover plate in the structure and its width of substrate can allow, and still, preferred described width value is all between 2 centimetres and 4 centimetres; It is the length value of setting arbitrarily that can reach the dependence test purpose that the length value of described cover plate and substrate can allow too, and still, preferred length value or the length value of saying recommendation are between 3 centimetres and 6 centimetres.
The concrete detection of this case micro-fluidic chip uses step as follows:
1, add sample liquid in the microtubule road, adding under the Micropump driving, various antigen molecules are caught by electrode surface corresponding antibodies in each passage, form immune complex by sandwich method desmoenzyme mark " two is anti-".
2, adopt the hyperchannel electroanalyzer, add electron mediators such as catechol, adopt ampere method detection " two is anti-" to go up enzyme and cause H
2O
2The electric current of reduction changes, and obtains various analyte content thus.
3, the result is carried out analysis-by-synthesis, cholera is diagnosed.
Advantage of the present invention is, integrated four working electrodes that are coated with four kinds of feature antibody materials top layers respectively on a micro-fluidic chip, these four working electrodes are respectively at four kinds of feature diagnosis albumen that detect cholera, this case micro-fluidic chip is a kind of micro-fluidic chip special that is applied to quick diagnosis deadly infectious disease cholera that can utilize cholera various features antibody to detect simultaneously, the design feature of its integrated structure and the specific shape of working electrode have determined the use of this chip to help to reduce sample liquid consumption, help accelerating the cholera diagnosis efficiency, help to reduce the cholera diagnostic fees and use.
Description of drawings
Fig. 1 is this case micro-fluidic chip embodiment structural configuration synoptic diagram, and what showed is the form of the perspective under the depression angle of this example structure, does not have to amplify in detail the pattern of describing described beading working electrode among the figure, does not also have the described annex of depicted in greater detail among the figure.
Among the figure, 1,2,10 is respectively three little liquid storage tanks that installation position is different, the 3rd, Y font three UNICOM roads, 4,7,11,14 is respectively different but four minim channels of formation parallel with one another UNICOM in parallel structure of installation position, the 5th, be installed in the beading working electrode in the minim channel 4 with cholera TP0821 antibody top layer, the 6th, be installed in the beading working electrode in the minim channel 7 with cholera TP0319 antibody top layer, the 12nd, be installed in the beading working electrode in the minim channel 11 with cholera TP0624 antibody top layer, the 13rd, be installed in the beading working electrode in the minim channel 14 with cholera O139 mycoprotein antibody top layer, the 8th, to electrode, the 9th, contrast electrode.
Embodiment
In this case embodiment that Fig. 1 showed, the structure of this micro-fluidic chip comprises that applying is installed in two plate objects together, two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip, on the position of the mutual applying between two plate objects, be equiped with capillary channel, and, three little liquid storage tanks, these three little liquid storage tanks are respectively little liquid storage tanks 1, little liquid storage tank 2 and little liquid storage tank 10, one end of capillary channel via Y font three UNICOM roads 3 respectively with little liquid storage tank 1 and little liquid storage tank 2 UNICOMs, the other end of capillary channel and remaining little liquid storage tank 10 UNICOMs, and, be installed in the described capillary channel working electrode on the diverse location in regular turn respectively and to electrode 8 and contrast electrode 9, the beading working electrode is made of conductive electrode and the antibody materials top layer that is coated on the conductive electrode, the structure of capillary channel is structure in parallel, this capillary channel that is structure in parallel is made of four minim channel parallel connections, these four minim channels are respectively minim channel 4 and minim channel 7 and minim channel 11 and minim channel 14, and, the quantity of beading working electrode is four, these four beading working electrodes are respectively beading working electrode 5 and beading working electrode 6 and beading working electrode 12 and beading working electrode 13, wherein, beading working electrode 5 is the beading working electrodes with cholera TP0821 antibody top layer that are installed in the minim channel 4, beading working electrode 6 is the beading working electrodes with cholera TP0319 antibody top layer that are installed in the minim channel 7, beading working electrode 12 is the beading working electrodes with cholera TP0624 antibody top layer that are installed in the minim channel 11, and beading working electrode 13 is the beading working electrodes with cholera O139 mycoprotein antibody top layer that are installed in the minim channel 14.Do not draw among Fig. 1 as associate members such as the microfluidic pump of annex and multiple tracks electrochemical workstations.Each beading working electrode in this routine structure and to electrode and contrast electrode can be respectively via special-purpose separately cable or say get lines crossed respectively with as the corresponding cable interface of the multiple tracks electrochemical workstation of annex or say the interface of getting lines crossed and connect.Each little liquid storage tank in this routine structure can be as required with as the microfluidic pump of annex UNICOM by any means.
Claims (6)
1. the special micro-fluidic chip for cholera diagnosis that has one-dimensional self-assembly magnetic bead chain electrodes, the structure of this micro-fluidic chip comprises that applying is installed in two plate objects together, described two plate objects are respectively the cover plate of micro-fluidic chip and the substrate of micro-fluidic chip, on the position of the mutual applying between described two plate objects, be equiped with capillary channel, and, three little liquid storage tanks, one end of capillary channel via Y font three UNICOM roads respectively with the little liquid storage tank of wherein two UNICOM, the other end of capillary channel and a remaining little liquid storage tank UNICOM, and, be installed in the described capillary channel working electrode on the diverse location in regular turn respectively and to electrode and contrast electrode, described working electrode is made of conductive electrode and the antibody materials top layer that is coated on the described conductive electrode, it is characterized in that, the structure of described capillary channel is structure in parallel, the described capillary channel that is structure in parallel is made of four minim channel parallel connections, and, the quantity of described working electrode is four, the installation position of these four working electrodes lays respectively in described four minim channels, and, antibody materials in these four its surface structures of working electrode is respectively four kinds of antibody materials, these four kinds of antibody materials are respectively cholera TP0821 antibody and cholera TP0319 antibody and cholera TP0624 antibody and cholera O139 mycoprotein antibody, and its pattern of each working electrode all is beading.
2. the special micro-fluidic chip for cholera diagnosis that has one-dimensional self-assembly magnetic bead chain electrodes according to claim 1, it is characterized in that, it is the tri-iron tetroxide material with conductive capability that described pattern is its material of catenate working electrode, and it is to add the accurate one-dimensional electric electrode that is spliced mutually under the magnetic field condition guiding by the magnetic bead of many tri-iron tetroxide materials that described pattern is catenate working electrode.
3. the special micro-fluidic chip for cholera diagnosis that has one-dimensional self-assembly magnetic bead chain electrodes according to claim 2, it is characterized in that, the diameter of described magnetic bead is between 20 nanometer to 2000 nanometers, and the number of the magnetic bead that is stitched together mutually that is contained in the structure of each described working electrode is between 100 to 100000.
4. the special micro-fluidic chip for cholera diagnosis that has one-dimensional self-assembly magnetic bead chain electrodes according to claim 1 is characterized in that, the thickness on described antibody materials top layer is between 2 nanometers and 100 nanometers.
5. the special micro-fluidic chip for cholera diagnosis that has one-dimensional self-assembly magnetic bead chain electrodes according to claim 1 is characterized in that, the described cover plate in the structure and its material of substrate are polymethylmethacrylate.
6. the special micro-fluidic chip for cholera diagnosis that has one-dimensional self-assembly magnetic bead chain electrodes according to claim 1, it is characterized in that, all between 2 centimetres and 4 centimetres, its length is all between 3 centimetres and 6 centimetres for described cover plate in the structure and its width of substrate.
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Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
| CN102580800A (en) * | 2012-03-01 | 2012-07-18 | 华东理工大学 | Method for designing and preparing electrochemical detection-microfluidic multichannel chip based on self-assembled monolayer technology |
| CN105891472A (en) * | 2015-01-18 | 2016-08-24 | 宁波大学 | Cheap cholera diagnosis micro-fluidic device with specific fluid flow transmission mode |
| CN105891471A (en) * | 2015-01-18 | 2016-08-24 | 宁波大学 | Cholera-diagnosis-used cheap microflow control device for conducting liquid flow driving through interface characteristics |
| CN106153932A (en) * | 2015-03-11 | 2016-11-23 | 宁波大学 | For the cholera diagnosis micro fluidic device driving the adnexa of liquid stream fast to remove |
| CN106290851A (en) * | 2015-05-11 | 2017-01-04 | 宁波大学 | A kind of easily-disassembled cholera diagnosis micro fluidic device of Dual Drive CGCM |
| CN107121545A (en) * | 2016-02-25 | 2017-09-01 | 葛宇杰 | Multichannel micro-fluidic chip device is used in the cholera diagnosis of dual drive coupling operating mode |
| CN107213926A (en) * | 2016-03-22 | 2017-09-29 | 李榕生 | The micro flow control chip device of ten Five-channel joint-detection Diagnostic Value of Several Serum Tumor Markers |
| CN107213923A (en) * | 2016-03-22 | 2017-09-29 | 李榕生 | Typical six kinds of tumor markers joint-detection micro flow control chip devices |
| CN107213925A (en) * | 2016-03-22 | 2017-09-29 | 李榕生 | Joint-detection male's classifiable tumor mark micro flow control chip device |
| CN107213924A (en) * | 2016-03-22 | 2017-09-29 | 李榕生 | The micro flow control chip device of a variety of classifiable tumor marks of women is detected simultaneously |
| CN107335478A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | Hard modifying interface is relied on to reduce the cheap micro fluidic device of cholera diagnosis of flow resistance |
| CN107335480A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | The tortuous cheap cholera diagnosis micro fluidic device of sap flow process route |
| CN107335486A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | The classifiable tumor markers in detecting micro flow control chip device that male is applicable |
| CN107340393A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | Six kinds of classifiable tumor mark multichannels while detection micro flow control chip device |
| CN107345932A (en) * | 2016-05-05 | 2017-11-14 | 李榕生 | The special cheap cholera diagnosis micro fluidic device of sap flow process mode |
| CN107349977A (en) * | 2016-05-10 | 2017-11-17 | 李榕生 | The cholera diagnosis micro fluidic device that can be fast removed for the annex for driving liquid stream |
| CN107486268A (en) * | 2016-06-13 | 2017-12-19 | 李榕生 | Six kinds of classifiable tumor mark multichannels while detection micro flow control chip device |
| CN107486262A (en) * | 2016-06-13 | 2017-12-19 | 李榕生 | General type is used for the micro flow control chip device for detecting Diagnostic Value of Several Serum Tumor Markers simultaneously |
| CN107486269A (en) * | 2016-06-13 | 2017-12-19 | 李榕生 | The classifiable tumor markers in detecting micro flow control chip device that women is applicable |
| CN107486266A (en) * | 2016-06-13 | 2017-12-19 | 李榕生 | The classifiable tumor markers in detecting micro flow control chip device that male is applicable |
| CN108236972A (en) * | 2018-02-26 | 2018-07-03 | 北京华科泰生物技术有限公司 | It is a kind of to be used to early diagnose joint-detection micro-fluidic chip of injury of kidney and its preparation method and application |
| CN110947436A (en) * | 2019-12-10 | 2020-04-03 | 清华大学 | Electrochemical detection device based on self-assembly technology and micro-fluidic chip technology |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
| CN102580800A (en) * | 2012-03-01 | 2012-07-18 | 华东理工大学 | Method for designing and preparing electrochemical detection-microfluidic multichannel chip based on self-assembled monolayer technology |
| CN105891472A (en) * | 2015-01-18 | 2016-08-24 | 宁波大学 | Cheap cholera diagnosis micro-fluidic device with specific fluid flow transmission mode |
| CN105891471A (en) * | 2015-01-18 | 2016-08-24 | 宁波大学 | Cholera-diagnosis-used cheap microflow control device for conducting liquid flow driving through interface characteristics |
| CN106153932A (en) * | 2015-03-11 | 2016-11-23 | 宁波大学 | For the cholera diagnosis micro fluidic device driving the adnexa of liquid stream fast to remove |
| CN106290851A (en) * | 2015-05-11 | 2017-01-04 | 宁波大学 | A kind of easily-disassembled cholera diagnosis micro fluidic device of Dual Drive CGCM |
| CN107121545A (en) * | 2016-02-25 | 2017-09-01 | 葛宇杰 | Multichannel micro-fluidic chip device is used in the cholera diagnosis of dual drive coupling operating mode |
| CN107213926A (en) * | 2016-03-22 | 2017-09-29 | 李榕生 | The micro flow control chip device of ten Five-channel joint-detection Diagnostic Value of Several Serum Tumor Markers |
| CN107213923A (en) * | 2016-03-22 | 2017-09-29 | 李榕生 | Typical six kinds of tumor markers joint-detection micro flow control chip devices |
| CN107213925A (en) * | 2016-03-22 | 2017-09-29 | 李榕生 | Joint-detection male's classifiable tumor mark micro flow control chip device |
| CN107213924A (en) * | 2016-03-22 | 2017-09-29 | 李榕生 | The micro flow control chip device of a variety of classifiable tumor marks of women is detected simultaneously |
| CN107335478A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | Hard modifying interface is relied on to reduce the cheap micro fluidic device of cholera diagnosis of flow resistance |
| CN107335480A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | The tortuous cheap cholera diagnosis micro fluidic device of sap flow process route |
| CN107335486A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | The classifiable tumor markers in detecting micro flow control chip device that male is applicable |
| CN107340393A (en) * | 2016-05-03 | 2017-11-10 | 宁波大学 | Six kinds of classifiable tumor mark multichannels while detection micro flow control chip device |
| CN107345932A (en) * | 2016-05-05 | 2017-11-14 | 李榕生 | The special cheap cholera diagnosis micro fluidic device of sap flow process mode |
| CN107349977A (en) * | 2016-05-10 | 2017-11-17 | 李榕生 | The cholera diagnosis micro fluidic device that can be fast removed for the annex for driving liquid stream |
| CN107486268A (en) * | 2016-06-13 | 2017-12-19 | 李榕生 | Six kinds of classifiable tumor mark multichannels while detection micro flow control chip device |
| CN107486262A (en) * | 2016-06-13 | 2017-12-19 | 李榕生 | General type is used for the micro flow control chip device for detecting Diagnostic Value of Several Serum Tumor Markers simultaneously |
| CN107486269A (en) * | 2016-06-13 | 2017-12-19 | 李榕生 | The classifiable tumor markers in detecting micro flow control chip device that women is applicable |
| CN107486266A (en) * | 2016-06-13 | 2017-12-19 | 李榕生 | The classifiable tumor markers in detecting micro flow control chip device that male is applicable |
| CN108236972A (en) * | 2018-02-26 | 2018-07-03 | 北京华科泰生物技术有限公司 | It is a kind of to be used to early diagnose joint-detection micro-fluidic chip of injury of kidney and its preparation method and application |
| CN110947436A (en) * | 2019-12-10 | 2020-04-03 | 清华大学 | Electrochemical detection device based on self-assembly technology and micro-fluidic chip technology |
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