CN107649208A - Strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material - Google Patents
Strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material Download PDFInfo
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- CN107649208A CN107649208A CN201610627453.6A CN201610627453A CN107649208A CN 107649208 A CN107649208 A CN 107649208A CN 201610627453 A CN201610627453 A CN 201610627453A CN 107649208 A CN107649208 A CN 107649208A
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
- B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/11—Orthomyxoviridae, e.g. influenza virus
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Abstract
The present invention relates to a kind of strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material, belong to analysis testing field.Many obstacles be present using dimethyl silicone polymer i.e. PDMS that is cheap and easily processing come the substrate for making hypotype swine flu diagnosis micro-fluidic chip；Its surface of the PDMS material is strongly hydrophobic, and targetedly surface is modified or surface modification, its effect are difficult to persistently, and this case aims to solve the problem that the serial relevant issues.Emphasis is, the selected PDMS with ecosystem surface of substrate, and fastening is positioned at the sample liquid stream terminal of the micro-fluidic chip its neighbor positions manually by the hinge-type fixture for being attached to miniature ultrasonic transducer units, interfacial tension is reduced with ultrasonic wave, strong absorbability using PDMS to ultrasonic wave simultaneously, reach ultrasonic intensity rapid decrement in short distance, so as to form interfacial tension difference at the both ends of the chip, sample liquid stream is thereby facilitated to be flowed along originally hydrophobic capillary channel to the terminal direction.
Description
Technical field
The present invention relates to a kind of strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material, belong to point
Analyse testing field.
Background technology
Multichannel micro-fluidic hypotype swine flu diagnostic techniques background, it is special to may refer to the grade inventions of CN 201110311127.1
Sharp application case.
Only for the microflow control technique overall general picture of itself, famous micro-fluidic expert Mr. Lin Ping Cheng may refer to not
The monograph " diagram Microfluid based Lab on a chip " gone out before long, the monograph is published via Science Press, and the monograph is to micro-fluidic
Past of technology, now, and, vision of the future etc. etc., suffer from detail, be deep into long discussion of detail.
So, the Important Problems of this case that to have a talk below concern.
The basic framework of micro-fluidic chip, including be etched with the substrate of small fluid course and fit together therewith
Cover plate, the small fluid course on the substrate, before upper cover plate is assembled, it is apparent on see to be exactly some micro-channels, to wait until
After covering cover plate thereon, just really closure forms the small fluid course, the conduit inner surface of the micro-channel together with
The part cover plate that surround the micro-channel forms described small fluid course together；So, it is clear that after assembling completes
The small fluid course, the major part of its inner surface area is the inner surface area of that micro-channel, in other words, should
The state or property of micro-channel inner surface substantially determine the integrality or property of the small fluid course；Therefore say, this
The inner surface state or inner surface property of micro-channel of the individual structure on substrate are key factors；In principle, it is any can
The material of its solid forms is kept or kept substantially, can be used to make substrate and cover plate, such as, it can act as substrate and lid
The material of piece can be monocrystalline silicon piece, quartz plate, sheet glass, high polymer for example dimethyl silicone polymer, polymethyl methacrylate,
Makrolon etc.；Certainly, the selection of substrate and the selection of cover plate be able to can also be differed with identical；From material consumption, make
From the point of view of difficulty and application popularization prospect etc. etc., not small difference, the especially choosing of that substrate between these materials be present
Material, have a great influence.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, at this
It is extremely simple that micro-channel is made on the substrate of sample, and the lower cost for material, base is made with the polydimethyl siloxane material
Piece, micro-channel, and the cover plate phase made with the cheap material such as glass or polypropylene or other plastic sheets are being suppressed or etched thereon
Coordinate, be a kind of more satisfactory selection seemingly；Certainly, patch material can also select to use cheap dimethyl silicone polymer
Material：So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, and making is extremely simple, seems
It should be extremely easy to popularize, promote.
But thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that alphabetical PDMS is referred to of abridging, itself are a kind of strong
Strong hydrophobic material, micro-channel is built on this material, if without being operated for the modified of the micro-channel surface, that
, after overall assembling is completed, that is, after covering cover plate, because the micro-channel its inner surface in structure occupies most liquid
The inner surface of circulation road, then, its strong hydrophobic property of the PDMS micro-channels inner surface, is deciding factor, it can cause
Similar to the aqueous solution the fine liquid stream of polar liquid by becoming very difficult, its flow resistance is big, or even in general is micro-
Pump is all difficult to promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar；
Therefore, among prior art, modification is modified particular for the micro-channel inner surface on the PDMS material, is necessary behaviour
Make；So, this is pretty troublesome for the modified operation of PDMS micro-channel inner surfacesThat falls nor this problem, is formed tight
Weight technology puzzlement, be another problem：PDMS polymer molecules inside its body phase of this PDMS material substrate have automatic
The characteristic diffuse to the surface, migrated, the spy that this substrate body phase inside PDMS polymer molecules are diffused to the surface, migrated automatically
Property, the state after modification by its inner surface of that micro-channel of surface modifying and decorating will be caused can not to maintain long enough
Time, the holding time for micro-channel its inner surface state after that is surface-modified be substantially only sufficient to complete laboratory internal
The time of test experiments needs；In other words, by surface modification or the PDMS micro-channel inner surfaces of surface modification, it is modified
The surface state that is formed can not be lasting afterwards or after saying modification, but soon automatically tends to or say and become surface modification again
Surface state before, the strong hydrophobic surface state of that script is returned in the shorter time, then, just think, this
The micro-fluidic chip of sample can largely make, mass storage, be widely popularized, and answer is it is obvious that is, impossible.This
Micro-channel on PDMS material, if not doing surface modification, similar to the aqueous solution the fine liquid stream of polar solvent can not pump it is logical
Cross, chip also cannot just use；And if having done surface modification, its state after modifying can not be persistently kept again, or together
Sample can not popularization and application.
So, how to accomplish that substrate can either be made using cheap PDMS material, and the microflute can be released
Road inner surface decorating state can not persistently, chip can not largely make, largely lay in and then be widely popularized and such a make ability
The puzzlement that the numerous professionals in domain are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, it is stated that, its surface is strongly hydrophobic above, this strong hydrophobic
Material surface and also have another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and
And these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into it is gradually deep, until
It is heavy to be trapped within the body phase of PDMS substrates, in fact, this process, partly it is also due to PDMS material body phase interior polymer
Molecule, which has, to be diffused to the surface, caused by travel motion；Such case, it can also be explained from another angle, i.e. continue not
Disconnectedly from inside PDMS body phases to those polymer molecules of its diffusion into the surface, migration, its result moved, be little by little by that
It has been involved in a bit by the large biological molecule of adsorption within the body phase of PDMS substrates, briefly, these adsorbed biologies
Macromolecular is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up the phenomenon of large biological molecule, its institute
Caused by influence, necessarily cause the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, the problem of PDMS substrates, is, its not only adsorption large biological molecule, and swallow up biological big point
Son, so, as the large biological molecule of experiment test object, its disappearance will not stop because surface saturation is adsorbed, and
It is, it is constantly adsorbed, also constantly swallowed up.
On PDMS substrates, its body phase is constantly swallowed up and tests showing for associated biomolecule macromolecular in related experiment test process
As, it is to say that another kind, which is explained, substantial amounts of Minute pores in PDMS body phases be present, associated biomolecule macromolecular by after adsorption,
Depression enters these Minute pores, and then is swallowed up；However, inventor thinks, those can allow the sky of miniature scale
Qi leel squeezes into the Minute pores therebetween, not equal to saying that they also can directly allow the large biological molecule of relative large scale to enter
Enter, both difference on yardstick are huge, must not make sweeping generalizations.Explanation is bypassed, in any case, as dependence test analysis object
Large biological molecule is adsorbed by PDMS substrate micro-channels inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is person in charge of reception at ceremonies
Phenomenon existing for sight.
, can be from containment PDMS surfaces pair in order to prevent swallow up effect of this PDMS substrate bodies relative to large biological molecule
The absorption of large biological molecule addresses, and method is chemically modified modification aiming at the PDMS material surface, for
For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described micro-channel part, by changing
The micro-channel inner surface of modification is learned, its absorption to large biological molecule can be contained, and then avoid large biological molecule
Swallowed up by PDMS substrate body phases；But or that old problem, that is, the chemical modification on PDMS material surface is modified
Surface state afterwards can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface, move automatically
The process of shifting, it soon can become that micro-channel inner surface state being modified by surface chemical modification again script strong and dredge
Water and the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS bases
Its micro-channel inner surface of piece is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, and can is enough
Reach and contain the absorption process of the PDMS substrate micro-channel inner surfaces to large biological molecule for a long time, and then prevent PDMS substrate body phases
The effect of swallowing up to large biological molecule so that related chip manufactured goods be able to maintain that one it is prolonged enough, reasonably guarantee the quality
Phase, it is exactly a very intractable problem.The problem equally makes this area numerous as another problem addressed above
Professional is entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.
Also be present many year in the problem, so far, also not yet properly settled.
The content of the invention
The technical problem to be solved by the invention is to provide a package solution, solves totally above
Two problems addressed, also, the solution is applied to hypotype swine flu diagnosis multichannel micro-fluidic chip field, shape
Into a kind of new hypotype swine flu diagnosis Multichannel device.
The present invention solves the technical problem by following scheme, and the device that the program provides is that a kind of strong hydrophobic PDMS makees
For the hypotype swine flu detection chip apparatus of substrate material, the structure of the device includes multichannel micro-fluidic chip, the miniflow
The structure of control chip includes being bonded to each other the substrate and cover plate of installing together, and the substrate and cover plate are plate object or sheet
The channel structure via mould pressing process or etching technics formation, the substrate are contained in thing, that face towards the cover plate of the substrate
Also contain and be connected and pierce being formed via mould pressing process, etching technics or simple drilling technology for the substrate with the channel structure
Window structure, be bonded to each other the substrate that is installed together and the cover plate and be built into jointly containing pipeline configuration and therewith
The micro-fluidic chip of connected liquid pool structure, the locations of structures of the pipeline are located at the interface area that the substrate is bonded to each other with the cover plate
Domain, its side of the window is blocked by the cover plate and opposite side opens, and the locations of structures of the window is exactly the structure bit of the liquid pool
Put, the quantity of the liquid pool is three, and its locations of structures of two liquid pools therein is located at the sample introduction end of the micro-fluidic chip, remaining
The liquid flowing in its chip when being located at the actual sample introduction test of the micro-fluidic chip of liquid pool its locations of structures terminal, the end
End is located remotely from each other with the sample introduction end, and one end of the pipeline is via the manifold shape turnout for being similarly positioned in the interface zone being bonded to each other
Respectively with the Liang Ge liquid pools UNICOM positioned at sample introduction end, the other end of the pipeline and the terminal positioned at the micro-fluidic chip
A remaining liquid pool UNICOM, and, the working electrode that is sequentially respectively installed in the pipeline on diverse location and to electricity
Pole and reference electrode, the working electrode is by conductive electrode and is attached on the conductive electrode and has embedded hypotype pig
The gold size sensitive membrane of influenza specific antigen is formed, and the construction of the pipeline is in parallel construction, the pipeline in parallel construction by
Three laterals are in parallel to be formed, and the quantity of the working electrode is three, the installation position difference position of three working electrodes
In in three laterals, and, the specific antigen point in its top layer gold size sensitivity membrane structure of three working electrodes
It is not the three kinds of hypotype swine flu antigenic substances that can be specifically bound to hypotype swine flu antibody, three kinds of antigenic substances are respectively
Hypotype swine flu specific antigen H1N1、H3N2And H1N2, its material of the working electrode is that gold material or thermal decomposition are conductive high
Sheet or thread is presented in molecule material, its pattern of the working electrode, and emphasis is that its material of the substrate is dimethyl silicone polymer
Material, its surface of the substrate are the surfaces of primary form, the surface of the primary form its be intended to refer to and do not pass through any table
The surface of the primary form of the material of face chemical modification or any surface chemical modification, the structure of the device also include hinge-type
Fixture, the hinge-type fixture its appearance profile likeness in form hinge, the hinge-type fixture by two hinges being mutually hinged with
And matched through a fastening screw of two hinges and one with the fastening screw and be socketed in one with the fastening screw
The nut of fastening and hand break loose manually that is used for risen is formed, and two hinges of the hinge-type fixture are respectively mutually drawn close with its tip
And the micro-fluidic chip is clamped, the tip is positioned in the locations of structures of the neighbouring terminal of the micro-fluidic chip, at least
Fixation is attached described in one of which in hinge and is equiped with miniature ultrasonic transducer units, and, the transmission of higher-order of oscillation electric signal
Cable, one end and the miniature ultrasonic transducer units of the higher-order of oscillation electric signal transmission cable link together；The hinge-type presss from both sides
Tool provides a function of facilitating the device to disassemble；Its major function of the miniature ultrasonic transducer units is real in micro-fluidic chip
During the sample introduction test of border, the ultrasonic wave launched using it is opened to reduce the interface between sample solution and the inwall of the pipeline
Power, can be compatible, also, using the sample introduction end and the terminal and the miniature ultrasonic transducer units installation position it
Between distance difference and the difference on its ultrasonic intensity experienced, its interfacial tension of sample introduction end described in induced synthesis with
Difference between its interfacial tension of the terminal, the interfacial tension difference between the micro-fluidic chip both ends can be micro-fluidic at this
Pressure gap is formed between the both ends of chip, the pressure gap can drive sample solution to the end flow；This is miniature super
The ultrasonic wave that acoustic wave transducer its function also includes being launched with it check large biological molecule contained in sample its described
Absorption on inner surface of pipeline, and then check the substrate of the dimethyl silicone polymer material its body phase and the large biological molecule is gulped down
Do not act on；Its function of the substrate of the dimethyl silicone polymer material is included with cover plate and working electrode and to electrode and reference electricity
Pole together builds the micro-fluidic chip, it is soft and have the substrate of the dimethyl silicone polymer material of elasticity its function also include with
Its property to the strong absorption of ultrasonic wave, ultrasonic wave is absorbed strongly, and thereby the micro-fluidic chip terminal to should
The rapid decrement of ultrasonic intensity is realized within limited short distance between sample introduction end, its locations of structures is located at the micro-fluidic core
A remaining liquid pool for the terminal of liquid flowing is also referred to as terminal liquid pool in its chip when the actual sample introduction of piece is tested, the terminal
Some high hydroscopic resin particles are filled with liquid pool, its opening end of the terminal liquid pool is covered by breathable microporous film.
Described miniature ultrasonic transducer units can certainly be all installed in two hinges；But only install one
Individual miniature ultrasonic transducer units are dealt with enough to be used.
The word of hinge one art-recognized meanings of itself are known.
The gold size sensitive membrane is to be sufficiently mixed chitosan gold size solution and hypotype swine flu specific antibody solution
It is even, with point sample instrument point sample or specified structure position is coated on, and form its drying and forming-film.Hypotype in the gold size sensitive membrane
Swine flu specific antibody be horseradish peroxidase or glucose oxidase mark hypotype swine flu antibody, the gold
Glue sensitive membrane has been included as fixing above-mentioned each hypotype swine flu specific antibody and introducing complementary medium therein, the auxiliary
Property medium such as chitosan, cellulose acetate, gelatin be therein a kind of or their mixture.
The pipeline and the lateral and the manifold shape turnout in the microfluidic chip structure, in it
Footpath size may each be arbitrarily selected size, still, for as far as possible less with prepare liquid sample and reduction reagent loss etc.
Consideration, the pipeline and the lateral and the manifold shape turnout preferably select capillary level passage, institute
The passage for stating capillary level implies that its internal diameter passage suitable with the internal diameter of the capillary on ordinary meaning.The capillary is in it
The shape of cross section of portion's passage can be arbitrary shape, and the shape of cross section is for example circular, oval, square, rectangle, bar
Shape, naturally it is also possible to it is the linear of bending arbitrarily be present, also, the interior shape of the capillary is with the extension of pipeline,
The shape of cross section of different parts can also allow to be different shapes.Only for the word of capillary one, its art-recognized meanings is public
Know.
What is be related in structure is the electrode of microsize to electrode and reference electrode, and its electrode shape, which may each be, appoints
The selected shape of meaning, the arbitrarily selected shape such as square piece shape, rectangular patch, strip or round sheet etc..It is described
Art-recognized meanings to electrode and the reference electrode vocabulary of itself are known.
It is related to several liquid pools in this case microfluidic chip structure, the liquid pool is the pond shape or capsule for transitional liquid storage
Shape constructs, and its shape of the inner chamber of each liquid pool may each be arbitrarily selected shape, and the cavity shape is for example round
Cylindrical cavity shape, square column type cavity-like, oblong cavity shape or spherical hollow space shape etc..The size of the liquid pool can be any
Selected size, still, in order to as far as possible less with prepare liquid sample and reduce reagent loss, the liquid pool be preferably capable of with
The liquid pool of the microminiature of capillary matching.
Only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field,
It is known.
Various sizes, variously-shaped ultrasonic transducer are commercially available；Its size of commercially available miniature ultrasonic transducer units
It may diminish to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface its
It is known general technology for the professional in ultrasonic technology field for body.This case is not to this expansion superfluous words.
Only with regard to naked PDMS substrates itself micro-channel molding or lithographic technique for, be open-and-shut known skill
Art；Similarly, the technology of hole-opening is even more known simple technique on naked PDMS substrates.This case is not also superfluous to this expansion
Speech.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the device can also include higher-order of oscillation electric signal generator；The higher-order of oscillation electric signal transmission cable
Its other end can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, come for the professional in ultrasonic technology field
Say, be simple and known；The higher-order of oscillation electric signal generator can customize to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 5 milliwatts and 9000 milliwatts
Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with
Between 12MHz.
Only for the word of high hydroscopic resin one art-recognized meanings of itself, come for the professional of chemical field
Say, be known.
The high hydroscopic resin is commercially available.
Only for the word of breathable microporous film one art-recognized meanings of itself, for the professional of technical field of membrane,
It is known.
The breathable microporous film is commercially available.
This case device can further include some annexes certainly, and the annex is such as multiple tracks electrochemical workstation
Deng the art-recognized meanings of the multiple tracks electrochemical workstation are known.The each work being related in this case microfluidic chip structure
Electrode and to electrode and reference electrode etc., special it can get lines crossed and the multiple tracks electrochemical workstation via corresponding respectively
The corresponding interface coupled.It is described that special to get lines crossed be for by each phase of each electrode and the multiple tracks electrochemical workstation
Answer the private cable that interface is coupled to each other.The micro-fluidic chip in this case device, its structure can also include micro-valve,
The quantity of the micro-valve is unlimited, and according to being actually needed, the micro-valve can be installed in any need in the microfluidic chip structure
Position to be mounted；The word of micro-valve one is for the professional of micro fluidic chip technical field, the art-recognized meanings of itself
It is known；The micro-valve itself manufacturing technology and the use of technology is also known；The component that the micro-valve is not required.
The diameter of the working electrode can allow to be that any setting is easily installed the suitable diameter used, still,
It is recommending or say preferable its scope of the diameter between 0.1 micron to 2000 microns；The length of the working electrode can
To allow to be that any setting is easily installed the length used, it is however recommended to or to say the preferable length its scope be 1
Micron is between 15000 microns.
It is installed in by spraying or point sample instrument point sample or the coating of other appropriate process described in the working electrode surface layer
Gold size sensitive membrane, its thicknesses of layers can allow be any setting treat sample measuring liquid occur electrical signals response thickness,
It is however recommended to thickness preferable thickness is between 10 nanometers and 200 nanometers in other words.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, such as：Polypropylene,
Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, for example do
Into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and realized in the extremely short distance to ultrasonic wave
Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip
Using dimethyl silicone polymer it is used as the cover plate, and this case technical scheme is allowed.
Positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal should
Air line distance between a remaining liquid pool can be arbitrarily selected suitable distance, still, the distance as needed
Preferred value is between 3 centimetres and 7 centimetres.
Described its thickness of cover plate and substrate can allow be any setting the thickness for being easy to assembling, the thickness of recommendation or say
Preferable thickness is between 1.0 millimeters and 5.0 millimeters.Less thickness is advantageous to save material.
The application method of this case micro-fluidic chip：
Itd is proposed first based on this case and the first public new liquid stream driving principle, its application of this case micro-fluidic chip are transported
Among work, the new liquid stream driving method is determined completely without involving any additional Micropump.
The interfacial tension difference that this case is formed between micro-fluidic chip both ends caused by the ultrasonic wave,
Driving liquid stream flows in the capillary channel of the triple channel micro-fluidic chip, right respectively using multi-channel electrochemical analyzer device
Three kinds of hypotype swine flu diagnostic antigens are detected.
Corresponding three kinds of hypotypes swine flu antibody is detected with three kinds of hypotype swine flu antigens, and with three kinds of hypotype swine flus
Antibody detects corresponding three kinds of hypotypes swine flu antigen, similarly, can diagnose corresponding hypotype swine flu；Its foundation
Principle be necessarily antigen, antibody and to be deposited in hypotype swine flu patient, and mutual Reversible binding forms reversible combination
Thing, therefore, detected antibody with antigen and thereby diagnosed corresponding hypotype swine flu, with going to detect antigen with antibody and diagnosing phase
The hypotype swine flu answered, can reach diagnostic purpose.Certainly, its corresponding electrode sensitive decorative layer of different means is in technology
Go up and differ.
The specific detection of this case micro-fluidic chip is as follows using step：
1st, blood serum sample liquid is added in micro-pipe road, under ultrasonic wave driving, various hypotype swine flu antibody molecules
The hypotype swine flu of the corresponding horseradish peroxidase-labeled embedded by gold size sensitive membrane on electrode surface in each passage is special
Property antigen capture.
2nd, the hypotype swine flu specific antigen of horseradish peroxidase-labeled resists with the hypotype swine flu in blood serum sample
Body forms immune complex.
3rd, using multi-channel electrochemical analyzer, the electron mediators such as catechol are added, it is above-mentioned using amperometric detection
Curent change caused by reaction, it is derived from the species and content of various analytes.
4th, result is subjected to comprehensive analysis, comprehensive diagnos is carried out to hypotype swine flu antibody.
It is an advantage of the invention that its close position positions the hinge-type folder in the terminal of the micro-fluidic chip
Tool, the miniature ultrasonic transducer units installed with institute's attaching in the hinge-type fixture its hinge, the low-power launched using it, height
The ultrasonic wave of frequent section so that without in strong hydrophobic micro-fluidic chip of surface chemical modification or surface chemical modification
Compatibility between its tube wall of portion's pipeline and the test object aqueous solution is significantly increased, and this is sample liquid stream by providing one
Realistic possibility；Meanwhile using its strong absorbability to ultrasonic wave of dimethyl silicone polymer substrate, in shorter distance
It is interior, it is, in from the terminal to the very short distance of only several centimeters yardsticks the sample introduction end, it is strong to reach ultrasonic wave
The rapid decrement of degree, the difference of the interfacial tension is thereby caused at the both ends of the micro-fluidic chip, and then, using this two
Pressure gap between its both ends for being formed of the difference of interfacial tension between end, driving sample liquid stream is in such a original
Flowed in this strong hydrophobic capillary channel to the terminal direction.By this case liquid stream drive scheme, entirely without must be to this
The substrate and its internal pipeline of dimethyl silicone polymer material carry out any surface chemical modification or chemical modification, altogether dispense with
The laborious procedures of the surface chemical modification or chemical modification；And setting for traditional Micropump etc is altogether dispensed with
It is standby；On the other hand, the ultrasonic wave of the low-power, high-frequency band, additionally it is possible to contain the large biological molecule in sample at this without modification
Naked its inner surface of pipeline of dimethyl silicone polymer substrate on absorption, and then contain its body of the dimethyl silicone polymer substrate
The effect of swallowing up of relatively described large biological molecule；The Reversible binding thing of the antigen, antibody and antigen and antibody is all certainly
Belong to the type of described large biological molecule；Because described suction-operated and the described effect of swallowing up effectively are contained,
Therefore, dependence test result will be better able to objectively reflect actual conditions；The effect of the low-power, high-frequency band ultrasonic wave,
Certainly also include facilitating the Reversible binding between antigen, antibody react it is quick reach, this causes dependence test operates can be with
Completed than faster speed.
Due to surface chemical modification or chemistry for its relevant surfaces of the dimethyl silicone polymer substrate need not be carried out
Modified operation, therefore, this surface chemical modification layer or chemically modified layer not need to exist, then, the poly dimethyl
Its body phase interior polymer molecule of siloxanes substrate constantly diffuses to the surface automatically, migrate caused by it to the surface chemistry
The damaging influence of decorative layer or chemically modified layer is also just not present.
It is related that the technical scheme of this case has dissolved its application to dimethyl silicone polymer substrate addressed above totally
A series of technical barriers.Based on this case scheme, the very cheap polydimethyl siloxane material of this kind is just possible to micro- at this
It is prepared by fluidic chip, production, using etc. field play bigger effect.
The miniature ultrasonic transducer units have been installed in fixation in the hinge-type fixture its hinge in this case structure, the structure
A function of facilitating the device to disassemble is provided, in this way, the hinge-type fixture is together with miniature ultrasonic appended in its hinge
Transducer just easily can be mutually disengaged with the micro-fluidic chip, then, the component that the part can freely depart from just can be good
Property is re-used cyclically many times；The architectural feature is advantageous to save the use cost of the device.
Brief description of the drawings
Fig. 1 is the schematic diagram of this case device embodiment chip part construction, it is shown that the vertical view of this structure
The structural form of chip internal pipeline and each electrode and each related liquid pool under angle, the hinge-type is not depicted in the figure
The miniature ultrasonic transducer units set up on fixture and its hinge and other annexes.
Fig. 2 is its rough outside side view of this case device.
In figure, 1,2,8 be the different liquid pool of three installation positions respectively, and 3 be manifold shape turnout, and 4,9,12 be installing respectively
Position is different but three laterals parallel with one another for forming UNICOM's structure in parallel, and 5 be its top layer being installed in lateral 4
Specific antigenic substance in gold size sensitivity membrane structure is hypotype swine flu specific antigen H1N1Working electrode, 10 be installing
The specific antigenic substance in its top layer gold size sensitivity membrane structure in lateral 9 is hypotype swine flu specific antigen
H3N2Working electrode, 11 be the specific antigenic substance in its top layer gold size sensitivity membrane structure being installed in lateral 12
For hypotype swine flu specific antigen H1N2Working electrode, 6 be to electrode, and 7 be reference electrode, and 13 be hinge-type fixture, 14 marks
Show linkwork position, 15 be fastening screw, and 16 be higher-order of oscillation electric signal transmission cable, and 17 be miniature ultrasonic transducing
Device, 18,23 be two different hinges of locations of structures respectively, and 19 be cover plate, and 20 be substrate, and 21 be sample introduction end, and 22 be terminal, 24
It is nut；The hinge-type clamp structure in legend is only the legend structure of signal, and actual its structure of the hinge-type fixture is unlimited
In the legend hinge-type clamp structure；Arrow in legend indicate the micro-fluidic chip its in actual motion, by both ends
Pressure differential drives, the flow direction of its sample liquid stream.
Embodiment
In this case that Fig. 1 and Fig. 2 are shown embodiment, the structure of the device includes multichannel micro-fluidic chip, should
The structure of micro-fluidic chip includes being bonded to each other the substrate 20 and cover plate 19 of installing together, and the substrate 20 and cover plate 19 are
What is formed via mould pressing process or etching technics contained in plate object or tablet, that face towards the cover plate 19 of the substrate 20
Channel structure, the substrate 20 also containing be connected with the channel structure and pierce the substrate 20 via mould pressing process, etch work
The window structure that skill or simple drilling technology are formed, is bonded to each other the substrate 20 being installed together and is built jointly with the cover plate 19
Into the micro-fluidic chip containing pipeline configuration and the liquid pool structure being attached thereto, the locations of structures of the pipeline is located at the substrate
20 interface zones being bonded to each other with the cover plate 19, its side of the window is blocked by the cover plate 19 and opposite side opens, the window
Locations of structures be exactly the liquid pool locations of structures, the quantity of the liquid pool is three, three liquid pools be respectively liquid pool 1,
Liquid pool 2, liquid pool 8, two liquid pools therein are the sample introduction end 21 that liquid pool 1 and liquid pool 2 its locations of structures are located at the micro-fluidic chip,
A remaining liquid pool is liquid flowing in its chip when liquid pool 8 its locations of structures is located at the micro-fluidic chip actual sample introduction test
Terminal 22, the terminal 22 is located remotely from each other with the sample introduction end 21, and one end of the pipeline is via being similarly positioned in the boundary being bonded to each other
The manifold shape turnout 3 in face region is liquid pool 1 and the UNICOM of liquid pool 2 with two liquid pools positioned at sample introduction end 21 respectively, the pipeline
The other end and a remaining liquid pool for the terminal 22 positioned at the micro-fluidic chip are the UNICOM of liquid pool 8, and, sequentially divide
The working electrode that is not installed in the pipeline on diverse location and to electrode 6 and reference electrode 7, the working electrode is by leading
Conductive electrodes and the gold size sensitive membrane for having embedded hypotype swine flu specific antigen being attached on the conductive electrode are formed,
The construction of the pipeline is in parallel construction, and the pipeline in parallel construction is made up of three lateral parallel connections, the work electricity
The quantity of pole is three, and the installation position of three working electrodes is located in three laterals respectively, and, this three
Specific antigen in its top layer gold size sensitivity membrane structure of working electrode is that hypotype swine flu antibody can be specifically bound respectively
Three kinds of hypotype swine flu antigenic substances, three kinds of antigenic substances are hypotype swine flu specific antigen H respectively1N1、H3N2And
H1N2, for specific expansion, 5 be the specific antigenic substance in its top layer gold size sensitivity membrane structure being installed in lateral 4
For hypotype swine flu specific antigen H1N1Working electrode, 10 be its top layer gold size sensitive membrane knot being installed in lateral 9
Specific antigenic substance in structure is hypotype swine flu specific antigen H3N2Working electrode, 11 be to be installed in lateral 12
Specific antigenic substance in its interior top layer gold size sensitivity membrane structure is hypotype swine flu specific antigen H1N2Work electricity
Pole, its material of the working electrode are gold material or thermal decomposition conducting polymer material, and its pattern of the working electrode is presented
Sheet is thread, and emphasis is, its material of the substrate 20 is dimethyl silicone polymer material, and its surface of substrate 20 is primary form
Surface, the surface of the primary form its be intended to refer to and do not pass through any surface chemical modification or any surface chemical modification
The material primary form surface, the structure of the device also includes hinge-type fixture 13, its profile of the hinge-type fixture 13
Profile is similar to hinge, and the hinge-type fixture 13 is by two hinges 18,23 for being mutually hinged and through two hinges
18th, 23 fastening screw 15 and the use for matching with the fastening screw 15 and being connected together with the fastening screw 15
Formed in the nut 24 of manual fastening and hand break loose, the nut 24 in legend is a kind of butterfly nut, this kind of butterfly nut
It is relatively convenient in practical operation, two hinges 18,23 of the hinge-type fixture 13 are respectively mutually drawn close with its tip and to clamp this micro-
Fluidic chip, the tip are positioned in the locations of structures of the neighbouring terminal 22 of the micro-fluidic chip, at least in
One hinge is to attach to fix in hinge 18 or hinge 23 to be equiped with miniature ultrasonic transducer units 17, and in this example, this is miniature
Ultrasonic transducer 17 is to attach to be installed in hinge 18, and, higher-order of oscillation electric signal transmission cable 16, higher-order of oscillation electricity
One end of signal transmission cable 16 links together with the miniature ultrasonic transducer units 17；The hinge-type fixture 13 provides one
The function of facilitating the device to disassemble；Its major function of the miniature ultrasonic transducer units 17 is in the actual sample introduction test of micro-fluidic chip
When, the ultrasonic wave launched using it reduces the interfacial tension between sample solution and the inwall of the pipeline, can
It is compatible, also, using between the sample introduction end 21 and the terminal 22 and the installation position of miniature ultrasonic transducer units 17
Difference in distance difference and its ultrasonic intensity experienced, its interfacial tension of sample introduction end 21 and institute described in induced synthesis
State the difference between terminal 22 its interfacial tension, the interfacial tension difference between the micro-fluidic chip both ends can be micro-fluidic at this
Pressure gap is formed between the both ends of chip, the pressure gap can drive sample solution to be flowed to the terminal 22；This is miniature
The ultrasonic wave that ultrasonic transducer 17 its function also includes being launched with it check large biological molecule contained in sample its
Absorption on the inner surface of pipeline, and then check its body phase of the substrate 20 of the dimethyl silicone polymer material and the biology is divided greatly
The effect of swallowing up of son；The substrate 20 of the dimethyl silicone polymer material its function include with cover plate 19 and working electrode 5,10,
11 and the micro-fluidic chip is together built to electrode 6 and reference electrode 7, soft dimethyl silicone polymer material for simultaneously having elasticity
Substrate 20 its function also include with its property to the strong absorption of ultrasonic wave, ultrasonic wave is absorbed strongly, and thereby exists
The micro-fluidic chip terminal 22 is to the fast express delivery that ultrasonic intensity is realized within the limited short distance between the sample introduction end 21
Subtract, its locations of structures is located at the described remaining of the terminal that liquid flows in its chip when the actual sample introduction of the micro-fluidic chip is tested
One liquid pool is also referred to as terminal liquid pool, is filled with some high hydroscopic resin particles in the terminal liquid pool, its opening end of the terminal liquid pool
Covered by breathable microporous film.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its try
The flow direction of sample liquid stream.
The miniature ultrasonic transducer units set up in the hinge-type fixture 13 and one hinge are depicted without in Fig. 1
17 and higher-order of oscillation electric signal transmission cable 16；Also, the higher-order of oscillation electric signal hair is depicted without in Fig. 1 and Fig. 2
The raw associate member such as device and multiple tracks electrochemical workstation.
Involved miniature ultrasonic transducer units 17 are commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal transmission cable 16 is commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also determine to relevant manufacturers
System.
Each working electrode in this example structure and can be respectively via each special electricity to electrode and reference electrode
Cable or say is being got lines crossed respectively with the corresponding cable interface of the multiple tracks electrochemical workstation as annex or saying interface connection of getting lines crossed.
Involved hinge-type fixture 13 itself can carry out the simple cutting transformation of the way using commercially available hinge.Hinge in legend
The structural form of formula fixture 13 is only an example, and the structural form of this case hinge-type fixture is not limited to the structure shape in the legend
State.
Capillary on usual expression and significance refers to hydrophilic capillary glass tube；Capillary channel involved by this case is
The pipeline of hydrophobic capillary form.
Claims (10)
- Hypotype swine flu detection chip apparatus of the hydrophobic PDMS of the last 1. as substrate material, the structure of the device include multichannel Micro-fluidic chip, the structure of the micro-fluidic chip include being bonded to each other the substrate and cover plate of installing together, the substrate and lid Piece is plate object or tablet, and that face towards the cover plate of the substrate is contained to be formed via mould pressing process or etching technics Channel structure, the substrate also containing be connected with the channel structure and pierce the substrate via mould pressing process, etching technics Or the window structure that simple drilling technology is formed, it is bonded to each other the substrate being installed together and has been built into jointly with the cover plate and contains The micro-fluidic chip of liquid pool structure for having pipeline configuration and being attached thereto, the locations of structures of the pipeline are located at the substrate and the lid The interface zone that piece is bonded to each other, its side of the window is blocked by the cover plate and opposite side opens, and the locations of structures of the window is just It is the locations of structures of the liquid pool, the quantity of the liquid pool is three, and its locations of structures of two liquid pools therein is located at the miniflow The sample introduction end of chip is controlled, its locations of structures of a remaining liquid pool is located at when the actual sample introduction of the micro-fluidic chip is tested in its chip The terminal of liquid flowing, the terminal is located remotely from each other with the sample introduction end, and one end of the pipeline is via being similarly positioned in what this was bonded to each other The manifold shape turnout of interface zone is micro- with being located at this with the Liang Ge liquid pools UNICOM positioned at sample introduction end, the other end of the pipeline respectively A remaining liquid pool UNICOM for the terminal of fluidic chip, and, sequentially it is respectively installed in diverse location in the pipeline On working electrode and to electrode and reference electrode, the working electrode is by conductive electrode and is attached to the electric conductivity The gold size sensitive membrane for having embedded hypotype swine flu specific antigen on electrode is formed, and the construction of the pipeline is in parallel construction, institute State and be made up of in the pipeline of parallel construction three lateral parallel connections, the quantity of the working electrode is three, this three work The installation position of electrode is located in three laterals respectively, and, its top layer gold size sensitive membrane of three working electrodes Specific antigen in structure is the three kinds of hypotype swine flu antigenic substances that can be specifically bound to hypotype swine flu antibody respectively, Three kinds of antigenic substances are hypotype swine flu specific antigen H respectively1N1、H3N2And H1N2, its material of the working electrode is yellow Sheet or thread is presented in golden material or thermal decomposition conducting polymer material, its pattern of the working electrode, it is characterised in that the base Its material of piece is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the surface of the primary form its The surface of the primary form for the material for not passing through any surface chemical modification or any surface chemical modification is intended to refer to, The structure of the device also includes hinge-type fixture, and hinge-type fixture its appearance profile likeness in form hinge, the hinge-type fixture is by phase Mutually hinged two hinges and through a fastening screw of two hinges and one and the fastening screw The nut of fastening and hand break loose manually that is used for matched somebody with somebody and connected together with the fastening screw is formed, and the two of the hinge-type fixture Individual hinge is respectively mutually drawn close with its tip and clamps the micro-fluidic chip, and the tip is positioned at the neighbouring institute of the micro-fluidic chip State in the locations of structures of terminal, fixation attached in a hinge at least in and is equiped with miniature ultrasonic transducer units, And higher-order of oscillation electric signal transmission cable, one end of the higher-order of oscillation electric signal transmission cable and the miniature ultrasonic transducing Device links together；The hinge-type fixture provides a function of facilitating the device to disassemble；The miniature ultrasonic transducer units its Major function be in the test of micro-fluidic chip actual sample introduction, the ultrasonic wave launched using it reduce sample solution with it is described Interfacial tension between the inwall of pipeline, can be compatible, also, miniature with this using the sample introduction end and the terminal Difference in the distance between ultrasonic transducer installation position difference and its ultrasonic intensity experienced, induced synthesis Difference between described its interfacial tension of sample introduction end and the terminal its interfacial tension, the boundary between the micro-fluidic chip both ends Face tension difference can form pressure gap between the both ends of the micro-fluidic chip, the pressure gap can drive sample solution to The end flow；The ultrasonic wave that its function of the miniature ultrasonic transducer units also includes being launched with it is checked contained in sample Large biological molecule its absorption on the inner surface of pipeline, and then check the substrate of the dimethyl silicone polymer material its body With respect to the effect of swallowing up of the large biological molecule；Its function of the substrate of the dimethyl silicone polymer material includes and cover plate and work Electrode and the micro-fluidic chip is together built to electrode and reference electrode, soft dimethyl silicone polymer material for simultaneously having elasticity Substrate its function also include with its property to the strong absorption of ultrasonic wave, ultrasonic wave is absorbed strongly, and thereby at this To the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end, it is tied the micro-fluidic chip terminal Structure position is located at a remaining liquid for the terminal of liquid flowing in its chip when the actual sample introduction of the micro-fluidic chip is tested Pond is also referred to as terminal liquid pool, is filled with some high hydroscopic resin particles in the terminal liquid pool, and its opening end of the terminal liquid pool is breathed freely Microporous barrier is covered.
- 2. strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material according to claim 1, its It is characterised by, the pipeline and the lateral and the manifold shape turnout are capillary channel.
- 3. strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material according to claim 1, its It is characterised by, the thermal decomposition conducting polymer is the electric conductivity formed by polyimides or polyacrylonitrile after anoxybiotic is heat-treated Material.
- 4. strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material according to claim 1, its Be characterised by, the width or diameter of the working electrode between 0.1 micron to 2000 microns, and, the working electrode Length between 1 micron to 15000 microns.
- 5. strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material according to claim 1, its It is characterised by, the thickness of the gold size sensitive membrane is between 10 nanometers and 200 nanometers.
- 6. strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material according to claim 1, its It is characterised by, the cover plate its material in structure is dimethyl silicone polymer material.
- 7. strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material according to claim 1, its Be characterised by, positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal should Air line distance between a remaining liquid pool is between 3 centimetres and 7 centimetres.
- 8. strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material according to claim 1, its It is characterised by, described its thickness of cover plate and substrate in structure is between 1.0 millimeters and 5.0 millimeters.
- 9. strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material according to claim 1, its Be characterised by, the structure of the device also includes higher-order of oscillation electric signal generator, the higher-order of oscillation electric signal transmission cable its The other end is connected with the higher-order of oscillation electric signal generator.
- 10. strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material according to claim 1, its It is characterised by, for its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units between 5 milliwatts and 9000 milliwatts, this is miniature The frequency of its ultrasonic wave operationally launched of ultrasonic transducer is between 100KHz and 12MHz.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610627453.6A CN107649208A (en) | 2016-07-26 | 2016-07-26 | Strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610627453.6A CN107649208A (en) | 2016-07-26 | 2016-07-26 | Strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material |
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| CN101817495A (en) * | 2010-03-25 | 2010-09-01 | 湖南大学 | Micro fluid control chip and preparation method and application thereof |
| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
| CN102645429A (en) * | 2012-04-23 | 2012-08-22 | 宁波大学 | Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device |
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2016
- 2016-07-26 CN CN201610627453.6A patent/CN107649208A/en not_active Withdrawn
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|---|---|---|---|---|
| CN101817495A (en) * | 2010-03-25 | 2010-09-01 | 湖南大学 | Micro fluid control chip and preparation method and application thereof |
| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
| CN102645429A (en) * | 2012-04-23 | 2012-08-22 | 宁波大学 | Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device |
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