CN107020156A - Driving liquid stream is easy to the AIDS diagnosis device of removal with component - Google Patents
Driving liquid stream is easy to the AIDS diagnosis device of removal with component Download PDFInfo
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- CN107020156A CN107020156A CN201610089762.2A CN201610089762A CN107020156A CN 107020156 A CN107020156 A CN 107020156A CN 201610089762 A CN201610089762 A CN 201610089762A CN 107020156 A CN107020156 A CN 107020156A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
- G01N33/56988—HIV or HTLV
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
- B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
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Abstract
The present invention relates to a kind of AIDS diagnosis device for driving liquid stream to be easy to removal with component, belong to analysis testing field.There is technology barrier with dimethyl silicone polymer i.e. PDMS that is cheap and easily processing to make the substrate of AIDS diagnosis micro-fluidic chip；Its surface of the material is strongly hydrophobic, and targetedly surface is modified or surface modification, and its effect is difficult to persistently, and this case aims to solve the problem that the serial relevant issues.This case main points are, the selected PDMS with ecosystem surface of substrate, and the elastic clip of miniature ultrasonic transducer units will be attached on its clamping limb with elastic force snap-in location in its neighbor positions of the test liquid stream terminal of the micro-fluidic chip, simultaneously, ultrasonic wave damper is installed at the sample introduction end of the micro-fluidic chip, reach ultrasonic intensity rapid decrement in short distance, so as to form interfacial tension difference at the two ends of the chip, test liquid stream is thereby facilitated to be flowed along originally hydrophobic capillary channel to the terminal direction.
Description
Technical field
The present invention relates to a kind of AIDS diagnosis device for driving liquid stream to be easy to removal with component, belong to analysis testing field.
Background technology
The micro-fluidic AIDS diagnosis technical background of associated multi-channel, may refer to the patent of invention such as CN 200910151219.0 Shen
Please case.
Only for the microflow control technique overall general picture of itself, it may refer to famous micro-fluidic expert Mr. Lin Ping Cheng and go out not long ago
Monograph " diagram Microfluid based Lab on a chip ", the monograph is published via Science Press, and the monograph is to microflow control technique
Past, now, and, in terms of vision of the future etc., suffer from detail, be deep into long discussion of detail.
So, the Important Problems of this case that to have a talk below concern.
The basic framework of micro-fluidic chip, including it is etched with the substrate of small fluid course and the cover plate fit together therewith,
Small fluid course on the substrate, assembling upper cover plate before, it is apparent on see to be exactly some micro-channels, wait until thereon
After covering cover plate, just real closure forms the small fluid course, and the conduit inner surface of the micro-channel is together with surround this
The part cover plate of micro-channel constitutes described small fluid course together；So, it is clear that this after assembling is completed is small
Fluid course, the major part of its inner surface area is the inner surface area of that micro-channel, in other words, in the micro-channel
The state or property on surface substantially determine the integrality or property of the small fluid course；Therefore say, this is built in base
The inner surface state or inner surface property of micro-channel on piece are key factors；In principle, it is any to keep or protecting substantially
The material of its solid forms is held, can be used to make substrate and cover plate, such as, the material that can act as substrate and cover plate can be with
It is monocrystalline silicon piece, quartz plate, sheet glass, high polymer such as dimethyl silicone polymer, polymethyl methacrylate, makrolon etc.
Deng；Certainly, the selection of substrate and the selection of cover plate be able to can also be differed with identical；From material consumption, manufacture difficulty and
From the point of view of in terms of application popularization prospect etc., between these materials exist not small difference, the especially selection of that substrate, influence compared with
Greatly.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, so
Substrate on make that micro-channel is extremely simple, and the lower cost for material makes substrate with the polydimethyl siloxane material,
Micro-channel is being suppressed or etched thereon, and is being engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes,
It is a kind of more satisfactory selection seemingly；Certainly, patch material can also select to use cheap polydimethyl siloxane material：
So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, makes extremely simple, seems and should also be as
It is extremely easy to popularize, promotes.
But, thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that the letter PDMS that abridges is referred to, itself are a kind of strong
Hydrophobic material, micro-channel is built on this material, if without being operated for the modified of the micro-channel surface, then,
After overall assembling is completed, that is, cover after cover plate, because the micro-channel its inner surface in structure occupies most liquid circulation
The inner surface in road, then, its strong hydrophobic property of the PDMS micro-channels inner surface, is deciding factor, it can cause class
Be similar to the aqueous solution the fine liquid stream of polar liquid by becoming very difficult, its flow resistance is big, or even general Micropump is all
It is difficult to promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar；
Therefore, among prior art, modification is modified particular for the micro-channel inner surface on the PDMS material, is necessary
Operation；So, this is pretty troublesome for the modified operation of PDMS micro-channel inner surfacesThat falls nor this problem, structure
Perplex into serious technical, be another problem：PDMS polymer molecules tool inside its body phase of this PDMS material substrate
There are PDMS polymer molecules inside the characteristic for diffusing to the surface, migrating automatically, this substrate body phase to diffuse to the surface, move automatically
The characteristic of shifting, will cause the state after modification by its inner surface of that micro-channel of surface modifying and decorating can not maintain foot
Enough long time, the holding time for micro-channel its inner surface state after that is surface-modified is substantially only sufficient to complete in laboratory
The time of portion's test experiments needs；In other words, by surface modification or the PDMS micro-channel inner surfaces of surface modification, its
The surface state that is formed can not be lasting after modification or after saying modification, but soon automatically tend to or say and become surface again and change
Surface state before property, returns to the strong hydrophobic surface state of that script in the shorter time, then, just think,
Such micro-fluidic chip can largely make, mass storage, be widely popularized, and answer is it is obvious that is, impossible.
Micro-channel on this PDMS material, can not pump similar to the fine liquid stream of polar solvent of the aqueous solution if not doing surface modification
Send and pass through, chip also cannot just be used；And if having done surface modification, the state after its modification can not be persistently kept again, also
Being equally can not popularization and application.
So, how to accomplish that substrate can either be made using cheap PDMS material, and table in the micro-channel can be released
Face decorating state can not persistently, chip can not largely make, largely lay in so that be widely popularized it is such a make this area it is numerous specially
The puzzlement that industry personnel are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, above it is stated that, its surface is strongly hydrophobic, this strong hydrophobic
Material surface and also another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and
And, these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into gradually deep, directly
It is trapped in heavy within the body phase of PDMS substrates, in fact, this process is partly also due to inside PDMS material body phase
Polymer molecule, which has, to be diffused to the surface, caused by travel motion；Such case, can also be explained from another angle, i.e.
Continuously from inside PDMS body phases to its diffusion into the surface, migration those polymer molecules, its result moved, be by
Gradually those are involved within the body phase of PDMS substrates by the large biological molecule of adsorption, briefly, these are inhaled
Attached large biological molecule is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up large biological molecule
Phenomenon, the influence caused by it necessarily causes the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, be the problem of PDMS substrates, its not only adsorption large biological molecule, and swallow up large biological molecule,
So, as the large biological molecule of experiment test object, its disappearance will not stop because the absorption of surface saturation, but, no
It is disconnected adsorbed, also constantly swallowed up.
On PDMS substrates in related experiment test process its body phase constantly swallow up test associated biomolecule macromolecular phenomenon, separately
It is to say that one kind, which is explained, there are substantial amounts of Minute pores in PDMS body phases, and associated biomolecule macromolecular is by after adsorption, depression
Into these Minute pores, and then swallowed up；However, inventor thinks, those can allow the air point of miniature scale
Son squeezes into the Minute pores therebetween, and being not equal to them also can directly allow that the large biological molecule of relative large scale enters, and two
Person's difference on yardstick is huge, must not make sweeping generalizations.Explanation is bypassed, in any case, the biology of object is analyzed as dependence test
Macromolecular is adsorbed by PDMS substrate micro-channels inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective
The phenomenon of presence.
, can be from containment PDMS surfaces to life in order to prevent this PDMS substrate bodies relative to the effect of swallowing up of large biological molecule
The absorption of thing macromolecular is addressed, and method is chemically modified modification aiming at the PDMS material surface, for
For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described micro-channel part, by changing
The micro-channel inner surface of modification is learned, its absorption to large biological molecule can be contained, and then avoid large biological molecule quilt
PDMS substrate body phases are swallowed up；But, or that old problem, that is, the chemical modification on PDMS material surface changes
Surface state after property can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface automatically,
The process of migration, soon can become that micro-channel inner surface state being modified by surface chemical modification again script strong and dredge
Water and the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS
Its micro-channel inner surface of substrate is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, can reach again
Growth stage contains absorption process of the PDMS substrate micro-channel inner surfaces to large biological molecule, and then prevents PDMS substrate body phases
The effect of swallowing up to large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life,
It is exactly a very intractable problem.The problem makes the numerous specialties in this area as another problem addressed above, equally
Personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.The hardly possible
Also be present many year in topic, so far, also not yet properly settled.
The content of the invention
The technical problems to be solved by the invention are to provide a package solution, solve what is addressed above totally
Two problems, also, the solution is used into multichannel micro-fluidic chip field applied to AIDS diagnosis, form a kind of new
AIDS diagnosis Multichannel device.
The present invention solves the technical problem by following scheme, and the device that the program is provided is that a kind of driving liquid stream component is easy
In the AIDS diagnosis device of removal, the structure of the device includes multichannel micro-fluidic chip, the structure bag of the micro-fluidic chip
Include installing substrate and cover plate together bonded to each other, the substrate and cover plate are plate object or tablet, the substrate towards
The channel structure via mould pressing process or etching technics formation is contained in that face of the cover plate, and the substrate also contains and the channel structure
It is connected and pierces the window structure via the formation of mould pressing process, etching technics or simple drilling technology of the substrate, it is bonded to each other
The substrate being installed together has been built into the miniflow containing pipeline configuration and the liquid pool structure being attached thereto jointly with the cover plate
Chip is controlled, the locations of structures of the pipeline is located at the substrate and cover plate interface zone bonded to each other, and its side of the window is covered by this
Piece is blocked and opposite side is opened, and the locations of structures of the window is exactly the locations of structures of the liquid pool, and the quantity of the liquid pool is three,
Its locations of structures of two liquid pools therein is located at the sample introduction end of the micro-fluidic chip, and its locations of structures of a remaining liquid pool is located at should
The terminal that liquid flows in its chip when the actual sample introduction of micro-fluidic chip is tested, the terminal is located remotely from each other with the sample introduction end, the pipeline
One end via be similarly positioned in the manifold shape turnout of the interface zone bonded to each other respectively with two liquid pools positioned at sample introduction end
UNICOM, the other end of the pipeline and a remaining liquid pool UNICOM for the terminal positioned at the micro-fluidic chip, and, according to
Sequence is respectively installed in working electrode in the pipeline on diverse location and to electrode and reference electrode, and the working electrode is by leading
Conductive electrodes and the gold size sensitive membrane for having embedded AIDS specific antigen being attached on the conductive electrode are constituted, the pipeline
Construction be in parallel construction, the pipeline in parallel construction is made up of four lateral parallel connections, the quantity of the working electrode
It is four, the installation position of four working electrodes is located in four laterals respectively, and, four working electrodes
Specific antigen in the sensitive membrane structure of its top layer gold size is that the four kinds of AIDS that can be specifically bound to aids antibody resist respectively
Original matter, four kinds of antigenic substances are AIDS specific antigen p24, gp41, gp120 and gp36, the work respectively
Its material of electrode is gold material or thermal decomposition conducting polymer material, and sheet or thread, weight is presented in its pattern of the working electrode
Point is that its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the primary form
Surface its be intended to refer to the primary form of the material not by any surface chemical modification or any surface chemical modification
Surface, the structure of the device also includes elastic clip, and two opposite clamping limb snap-in locations of the elastic clip are in the micro-fluidic core
Fixation is attached on the position of the neighbouring terminal of piece, a clamping limb at least in and is equiped with miniature ultrasonic transducing
Device, and, higher-order of oscillation electric signal transmission cable, one end and the miniature ultrasonic of the higher-order of oscillation electric signal transmission cable are changed
Energy device links together；The elastic clip facilitates the function that the device is disassembled there is provided one；Its is main for the miniature ultrasonic transducer units
Function is that in the test of micro-fluidic chip actual sample introduction, the ultrasonic wave launched using it reduces sample solution and the pipeline
Interfacial tension between inwall, can be compatible, also, utilizes the sample introduction end and the terminal and the miniature ultrasonic
Difference in the distance between transducer installation position difference and its ultrasonic intensity experienced, sample introduction described in induced synthesis
Hold the interfacial tension difference between the difference between its interfacial tension and the terminal its interfacial tension, the micro-fluidic chip two ends
Pressure gap can be formed between the two ends of the micro-fluidic chip, the pressure gap can drive sample solution to the terminal stream
It is dynamic；Its function of the miniature ultrasonic transducer units also includes checking large biological molecule contained in sample with its ultrasonic wave launched
Its absorption on the inner surface of pipeline, and then check its body phase of the substrate of the dimethyl silicone polymer material to the biology greatly point
The effect of swallowing up of son；Its function of the substrate of the dimethyl silicone polymer material is included with cover plate and working electrode and to electrode and ginseng
Together build the micro-fluidic chip than electrode, it is soft and have the substrate of the dimethyl silicone polymer material of elasticity its function and also include
With its property to the strong absorption of ultrasonic wave, ultrasonic wave is absorbed strongly, and thereby the micro-fluidic chip terminal to should
The rapid decrement of ultrasonic intensity is realized within limited short distance between sample introduction end；And, ultrasonic wave damper, the ultrasound
Its profile of ripple damper is in block, tabular or bar-shaped；Its material of the ultrasonic wave damper is microcellular rubber, sponge rubber or poly- ammonia
Ester micro-pore elastomer；And, another elastic clip, another elastic clip is with its gripping strength by the sample introduction of the micro-fluidic chip
End with the ultrasonic wave damper it is bonded to each other clip together, the ultrasonic wave damper with its material brought to ultrasonic energy
Strong consumption, further support within micro-fluidic chip terminal to the limited short distance between the sample introduction end reality
The rapid decrement of existing ultrasonic intensity, and thereby further expand described its interfacial tension of sample introduction end and its interfacial tension of the terminal
Between difference.
Described miniature ultrasonic transducer units can certainly be all installed on the elastic clip two clamping limb；But, only fill
If a miniature ultrasonic transducer units are dealt with and used enough.
The word of elastic clip one art-recognized meanings of itself are known.
Another elastic clip is naturally also elastic clip.
The elastic clip and another elastic clip refer respectively to the elastic clip on two different structure positions.
The gold size sensitive membrane is to be sufficiently mixed chitosan gold size solution and AIDS specific antigen solution uniformly, uses point sample
Instrument point sample is coated on specified structure position, and forms its drying and forming-film.AIDS disease-specific in the gold size sensitive membrane resists
Original is the AIDS antigen of horseradish peroxidase or glucose oxidase mark, and the gold size sensitive membrane has been included as fixing
Above-mentioned each AIDS specific antigen and introduce complementary medium therein, the complementary medium such as chitosan, acetate fiber
Element, gelatin is therein a kind of or their mixture.
The pipeline and the lateral and the manifold shape turnout in the microfluidic chip structure, its internal diameter chi
It is very little to may each be arbitrarily selected size, still, use the consideration in terms of prepare liquid sample and reduction reagent loss less for trying one's best,
The passage of capillary level, the capillary level are preferably selected in the pipeline and the lateral and the manifold shape turnout
Passage imply that its internal diameter passage suitable with the internal diameter of the capillary on ordinary meaning.Its inner passage of capillary it is transversal
Face shape can be arbitrary shape, the shape of cross section is for example circular, oval, square, rectangle, bar shaped, certainly
Can be arbitrarily exist bending it is linear, also, the capillary interior shape with the extension of pipeline, different parts
Shape of cross section can also allow to be different shapes.Only for the word of capillary one, its art-recognized meanings is known.
What is be related in structure is the electrode of microsize to electrode and reference electrode, and its electrode shape may each be any choosing
Fixed shape, the arbitrarily selected shape such as square piece shape, rectangular patch, strip or round sheet etc..It is described to electricity
The art-recognized meanings of pole and the reference electrode vocabulary of itself are known.
It is related to several liquid pools in this case microfluidic chip structure, the liquid pool is the pond shape or scrotiform structure for transitional liquid storage
Make, its shape of the inner chamber of each liquid pool may each be arbitrarily selected shape, the cavity shape such as cylindrical empty
Cavity-like, square column type cavity-like, oblong cavity shape or spherical hollow space shape etc..The size of the liquid pool can be arbitrarily selected
Size, still, in order to use prepare liquid sample and reduction reagent loss less as far as possible, the liquid pool is preferably capable of and capillary
The liquid pool of the microminiature of matching.
It is public only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field
Know.
Various sizes, variously-shaped ultrasonic transducer are commercially available；Commercially available miniature ultrasonic transducer units its sizes can be with
It is small to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface itself and
Speech, is known general technology for the professional in ultrasonic technology field.This case is not to this expansion superfluous words.
Only with regard to naked PDMS substrates itself micro-channel molding or lithographic technique for, be open-and-shut known technology；Together
Sample, the technology of hole-opening is even more known simple technique on naked PDMS substrates.This case is not also to this expansion superfluous words.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the device can also include higher-order of oscillation electric signal generator；Its is another for the higher-order of oscillation electric signal transmission cable
One end can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, for the professional in ultrasonic technology field, be
It is simple and known；The higher-order of oscillation electric signal generator can be customized to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units be between 5 milliwatts and 5000 milliwatts it
Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with
Between 12MHz.
This case device can further include some annexes, the annex such as multiple tracks electrochemical workstation etc., institute certainly
The art-recognized meanings for stating multiple tracks electrochemical workstation are known.Each working electrode for being related in this case microfluidic chip structure and
, can be respectively via corresponding special the corresponding interface got lines crossed with the multiple tracks electrochemical workstation to electrode and reference electrode etc.
Coupled.It is described that special to get lines crossed be for each the corresponding interface of each electrode and the multiple tracks electrochemical workstation is carried out into phase
The private cable being mutually coupled with.The micro-fluidic chip in this case device, its structure can also include micro-valve, the number of the micro-valve
Amount is not limited, according to actual needs, and the micro-valve can be installed in any required position installed in the microfluidic chip structure；
The word of micro-valve one is for the professional of micro fluidic chip technical field, and the art-recognized meanings of itself are known；The micro-valve
The manufacturing technology of itself and the use of technology is also known；The component that the micro-valve is not required.
The diameter of the working electrode can allow to be that any setting is easily installed the suitable diameter used, it is however recommended to
Or say preferred its scope of the diameter between 0.1 micron to 2000 microns；The length of the working electrode can permit
Permitted to be that any setting is easily installed the length used, it is however recommended to or to say preferred its scope of the length be at 1 micron
To between 15000 microns.
The gold size for being installed in the working electrode surface layer by spraying or point sample instrument point sample or the coating of other appropriate process is quick
Feel film, its thicknesses of layers can allow be any setting treat sample measuring liquid occur electrical signals response thickness, still, push away
Preferred thickness is between 10 nanometers and 200 nanometers in other words for the thickness recommended.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, for example：Polypropylene, glass
Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, such as do
Realized into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and in the extremely short distance to ultrasonic wave
Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip
It is used as the cover plate using dimethyl silicone polymer, is also that this case technical scheme is allowed.
Positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal this is remaining
Air line distance between one liquid pool can be arbitrarily selected suitable distance as needed, and still, the preferred value of the distance is
Between 3 centimetres and 7 centimetres.
Described its thickness of cover plate and substrate can allow be any setting be easy to assembling thickness, the thickness of recommendation or say preferably
Thickness be between 1.0 millimeters and 5.0 millimeters.Less thickness is conducive to saving material.
Described its size of ultrasonic wave damper is not limited, and described its length of ultrasonic wave damper, width, thickness may each be basis
Need the arbitrary size of setting.
It is known in field of rubber technology only for the word of microcellular rubber one art-recognized meanings of itself.
The microcellular rubber is commercially available.
It is known in field of rubber technology only for the word of sponge rubber one art-recognized meanings of itself.
The sponge rubber is commercially available.
It is known in technical field of macromolecules only for the word of microporous polyurethane elastomer one art-recognized meanings of itself.
The polyurethane micropore elastomer material is commercially available.
The microcellular rubber preferred microporous silicon rubber or micropore fluorubber.
It is known in field of rubber technology only for the word of micropore silicon rubber one art-recognized meanings of itself.
The microporous silicon Rubber market is on sale.
It is known in field of rubber technology only for the word of micropore fluorubber one art-recognized meanings of itself.
The micropore fluorubber is commercially available.
Another described elastic clip, naturally also elastic clip；The word of elastic clip one art-recognized meanings of itself are known.
Various elastic clip market is all on sale.
The application method of this case micro-fluidic chip：
Proposed first based on this case and the first public New stream driving principle, its application running of this case micro-fluidic chip
In, the New stream driving method is determined completely without involving any additional Micropump.
The interfacial tension difference of this case to be formed between micro-fluidic chip two ends caused by the ultrasonic wave, drives liquid
Stream flows in the capillary channel of the four-way micro-fluidic chip, using four-way electrochemical analytical instrument respectively to four kinds of AIDS
Sick antibody is detected.
Corresponding four kinds of aids antibodies are detected with four kinds of AIDS antigens, with detecting corresponding four with four kinds of aids antibodies
AIDS antigen is planted, similarly, can diagnosis of aids；The principle of its foundation is necessarily resisted in AIDS patient
Former, antibody is simultaneously deposited, and mutually Reversible binding, is formed reversible conjugate, therefore, antibody is detected with antigen and is thereby diagnosed
AIDS, with removing detecting antigen and diagnosis of aids with antibody, can reach diagnostic purpose.Certainly, its phase of different means
The electrode sensitive decorative layer answered technically and is differed.
The specific detection of this case micro-fluidic chip is as follows using step：
1st, blood serum sample liquid is added in micro-pipe road, under ultrasonic wave driving, various aids antibody molecules are by each passage
The AIDS specific antigen capture for the corresponding horseradish peroxidase-labeled that gold size sensitive membrane is embedded on middle electrode surface.
2nd, the aids antibody in the AIDS specific antigen and blood serum sample of horseradish peroxidase-labeled forms immune compound
Thing.
3rd, using multi-channel electrochemical analyzer, the electron mediators such as catechol are added, using the above-mentioned reaction of amperometric detection
Caused curent change, is derived from the species and content of various analytes.
4th, result is subjected to comprehensive analysis, comprehensive diagnos is carried out to aids antibody.
It is an advantage of the invention that in elastic clip described in the terminal of the micro-fluidic chip its close position snap-in location, with
The miniature ultrasonic transducer units installed, the low-power launched using it, high-frequency band are attached on the clamping limb of the elastic clip
Ultrasonic wave so that without its pipe of strong hydrophobic micro-fluidic chip internal pipeline of surface chemical modification or surface chemical modification
Compatibility between wall and the test object aqueous solution is significantly increased, and this is test liquid stream by there is provided a realistic possibility；
Meanwhile, using its strong absorbability to ultrasonic wave of dimethyl silicone polymer substrate, in shorter distance, it is,
In from the terminal to the very short distance of only several centimeters yardsticks the sample introduction end, reach the fast express delivery of ultrasonic intensity
Subtract, the difference of the interfacial tension is thereby caused at the two ends of the micro-fluidic chip, and then, utilize the boundary between the two ends
Pressure gap between its two ends for being formed of the difference of face tension force, driving test liquid stream is strong hydrophobic in such a script
Flowed in capillary channel to the terminal direction.By this case liquid stream drive scheme, entirely without must be to the dimethyl silicone polymer
The substrate and its internal pipeline of material carry out any surface chemical modification or chemical modification, have altogether dispensed with the surface chemical modification
Or the laborious procedures of chemical modification；And altogether dispense with the equipment of traditional Micropump etc；On the other hand, the low work(
The ultrasonic wave of rate, high-frequency band, additionally it is possible to contain the large biological molecule in sample in the literalness naked dimethyl silicone polymer
Absorption on its inner surface of pipeline of substrate, and then contain that its body phase of the dimethyl silicone polymer substrate is gulped down to the large biological molecule
Do not act on；The Reversible binding thing of the antigen, antibody and antigen and antibody is all the class for belonging to described large biological molecule certainly
Type；Because described suction-operated and the described effect of swallowing up effectively are contained that therefore, dependence test result more will can
It is enough objectively to reflect actual conditions；The effect of the low-power, high-frequency band ultrasonic wave, certainly also include facilitate antigen, antibody it
Between Reversible binding reaction quick reaching, this causes dependence test operation can be to be completed than faster speed.
Due to the surface chemical modification for the dimethyl silicone polymer substrate its relevant surfaces or chemical modification behaviour need not be carried out
Make, therefore, this surface chemical modification layer or chemically modified layer not need to exist, then, the dimethyl silicone polymer
Its body phase interior polymer molecule of substrate constantly diffuses to the surface automatically, migrate caused by it to surface chemical modification layer or
The damaging influence of chemically modified layer is also just not present.
This case its installation position is located at the ultrasonic wave damper at the sample introduction end, from its terminal of this case micro-fluidic chip to
Said on the sport technique segment that ultrasonic intensity rapid decrement is realized in the such short distance in sample introduction end, its effect of the ultrasonic wave damper is suitable
In the size expansion device of micro-fluidic chip, in other words, the ultrasonic wave damper its function as and significantly extend miniflow
Its terminal of chip is controlled the distance between to sample introduction end, is the equal of from several centimetres of extension of script to more than ten centimetres of length
Or twenties centimetres of length or longer length, so so that described terminal to the ultrasonic wave between sample introduction end in short distance
The sport technique segment of intensity rapid decrement can relatively easily be reached, also, the size expansion device namely the ultrasonic wave damper
Demolition, installation are convenient, it is important that it can also be reused.
The technical scheme of this case has dissolved its application related one to dimethyl silicone polymer substrate addressed above totally
Row technical barrier.Based on this case scheme, this kind very cheap polydimethyl siloxane material is just possible in the micro-fluidic chip
Prepare, production, using etc. field play bigger effect.
The miniature ultrasonic transducer units have been installed in fixation on the elastic clip its clamping limb in this case structure, this architecture provides
One facilitates the function that the device is disassembled, in this way, the elastic clip just can facilitate together with miniature ultrasonic transducer units appended thereon
Ground is mutually disengaged with the micro-fluidic chip, then, the component that the part can freely depart from is reused in which just can circulate benignly to be permitted
Repeatedly；The architectural feature is conducive to saving the use cost of the device.
Brief description of the drawings
Fig. 1 is the schematic diagram of this case device embodiment chip part construction, it is shown that the depression angle of this structure
Under chip internal pipeline and each electrode and each related liquid pool structural form, do not depict in the figure elastic clip and its
On the miniature ultrasonic transducer units set up and other annexes.
Fig. 2 is its rough outside side view of this case device.
In figure, 1,2,10 be the different liquid pool of three installation positions respectively, and 3 be manifold shape turnout, 4,7,11,14
It is that installation position is different but four laterals parallel with one another for forming UNICOM's structure in parallel respectively, 5 be to be installed in lateral 4
The working electrode that specific antigenic substance in the interior sensitive membrane structure of its top layer gold size is AIDS specific antigen p24,6 are
Specific antigenic substance in the sensitive membrane structure of its top layer gold size being installed in lateral 7 is AIDS specific antigen
Gp41 working electrode, 12 be the specific antigen thing in the sensitive membrane structure of its top layer gold size being installed in lateral 11
Matter is AIDS specific antigen gp120 working electrode, and 13 be that its top layer gold size being installed in lateral 14 is sensitive
The working electrode that specific antigenic substance in membrane structure is AIDS specific antigen gp36,8 be that, to electrode, 9 be reference
Electrode, 15 be elastic clip, and 16,21 be two clamping limbs of elastic clip respectively, and 17 be the terminal of the micro-fluidic chip,
18 be the sample introduction end of the micro-fluidic chip, and 19 be the substrate of dimethyl silicone polymer material, and 20 be cover plate, and 22 be micro-
Type ultrasonic transducer, 23 be higher-order of oscillation electric signal transmission cable, and 24 be tension spring；The spring clamp structure in legend
Only it is the legend structure of signal, actual spring clamp structure is not limited to the legend spring clamp structure；Arrow sign in legend should
Micro-fluidic chip its in actual motion, by pressure at two ends difference drive, the flow direction of its test liquid stream.It is depicted without in legend
Also another elastic clip is depicted without in the ultrasonic wave damper, legend.
Embodiment
In this case that Fig. 1 and Fig. 2 are shown embodiment, the structure of the device includes multichannel micro-fluidic chip, and this is micro-
The structure of fluidic chip includes the substrate 19 and cover plate 20 of installing bonded to each other together, and the substrate 19 and cover plate 20 are
Contain what is formed via mould pressing process or etching technics in plate object or tablet, that face towards the cover plate 20 of the substrate 19
Channel structure, the substrate 19 also containing be connected with the channel structure and pierce the substrate via mould pressing process, etching technics
Or the window structure of simple drilling technology formation, substrate 19 bonded to each other being installed together is built into jointly with the cover plate 20
Micro-fluidic chip containing pipeline configuration and the liquid pool structure being attached thereto, the liquid pool is liquid pool 1, liquid pool 2, liquid pool
10, the locations of structures of the pipeline is located at the substrate 19 and the cover plate 20 interface zone bonded to each other, and its side of the window is by this
Cover plate 20 is blocked and opposite side is opened, and the locations of structures of the window is exactly the structure position of the liquid pool 1, liquid pool 2, liquid pool 10
Put, the quantity of the liquid pool is three, two liquid pools therein are liquid pool 1 and its locations of structures of liquid pool 2 positioned at the micro-fluidic core
The sample introduction end 18 of piece, a remaining liquid pool is that liquid pool 10 its locations of structures is located at when the actual sample introduction of the micro-fluidic chip is tested it
The terminal 17 that liquid flows in chip, the terminal 17 is located remotely from each other with the sample introduction end 18, and one end of the pipeline is via same position
In the manifold shape turnout 3 of the interface zone bonded to each other respectively with the liquid pool 1 positioned at sample introduction end 18 and the UNICOM of liquid pool 2,
A remaining liquid pool for the terminal 17 of the other end of the pipeline with being located at the micro-fluidic chip is the UNICOM of liquid pool 10, with
And, the working electrode that is sequentially respectively installed in the pipeline on diverse location and to electrode 8 and reference electrode 9, the work
Make electrode by conductive electrode and the gold size sensitive membrane for having embedded AIDS specific antigen being attached on the conductive electrode
Constitute, the construction of the pipeline is in parallel construction, and the pipeline in parallel construction is made up of four lateral parallel connections, the work
The quantity for making electrode is four, and the installation position of four working electrodes is located in four laterals respectively, four works
It is working electrode 5, working electrode 6, working electrode 12, working electrode 13 respectively to make electrode, and, four working electrodes
Specific antigen in the sensitive membrane structure of its top layer gold size is that the four kinds of AIDS that can be specifically bound to aids antibody resist respectively
Original matter, four kinds of antigenic substances are AIDS specific antigen p24, gp41, gp120 and gp36 respectively, are specifically opened up
Come and say, 5 be that specific antigenic substance in the sensitive membrane structure of its top layer gold size being installed in lateral 4 is special for AIDS
Specific Antigen p24 working electrode, 6 be that specificity in the sensitive membrane structure of its top layer gold size being installed in lateral 7 resists
Original matter is AIDS specific antigen gp41 working electrode, and 12 be that its top layer gold size being installed in lateral 11 is quick
Feel the working electrode that the specific antigenic substance in membrane structure is AIDS specific antigen gp120,13 be to be installed in branched pipe
The specific antigenic substance in the sensitive membrane structure of its top layer gold size in road 14 is electric for AIDS specific antigen gp36 work
Pole, the working electrode 5, working electrode 6, working electrode 12, working electrode 13 its material are gold material or thermal decomposition
Conducting polymer material, the working electrode 5, working electrode 6, working electrode 12, its pattern presentation piece of working electrode 13
Shape is thread, and emphasis is, its material of the substrate 19 is dimethyl silicone polymer material, and its surface of substrate 19 is primary shape
The surface of state, the surface of the primary form its be intended to refer to not pass through any surface chemical modification or any surface chemical modification
The material primary form surface, the structure of the device also includes elastic clip 15, two opposite folders of the elastic clip 15
Gripping arm 16,21 snap-in locations are in the position of the neighbouring terminal 17 of the micro-fluidic chip, described in one at least in
Attach fixation on clamping limb to be equiped with miniature ultrasonic transducer units 22, legend, the miniature ultrasonic transducer units 22 are to attach admittedly
Surely on installing one of which clamping limb 21, and, higher-order of oscillation electric signal transmission cable 23, the higher-order of oscillation electric signal
One end of transmission cable 23 links together with the miniature ultrasonic transducer units 22；The elastic clip 15 provides a convenience should
The function that device is disassembled；Its major function of the miniature ultrasonic transducer units 22 is the profit in micro-fluidic chip actual sample introduction test
The ultrasonic wave launched with it reduces the interfacial tension between sample solution and the inwall of the pipeline, can be compatible, and
And, utilize the distance between the sample introduction end 18 and the terminal 17 and the installation position of miniature ultrasonic transducer units 22 difference
Difference on different and its ultrasonic intensity for being experienced, its interfacial tension of sample introduction end 18 described in induced synthesis and the terminal
Difference between 17 its interfacial tension, micro-fluidic chip two ends are that the interfacial tension between sample introduction end 18 and terminal 17 is poor
Different can be to form pressure gap between sample introduction end 18 and terminal 17 at the two ends of the micro-fluidic chip, and the pressure gap can drive
Dynamic sample solution flows to the direction of terminal 17；Its function of the miniature ultrasonic transducer units 22 also includes what is launched with it
Ultrasonic wave checks in sample contained large biological molecule its absorption on the inner surface of pipeline, and then checks the poly dimethyl
Swallow up effect of its body phase of the substrate 19 of siloxanes material to the large biological molecule；The substrate of the dimethyl silicone polymer material
19 its function include and cover plate 20 and working electrode 5, working electrode 6, working electrode 12, working electrode 13 and to electrode 8
It is soft and have the substrate of the dimethyl silicone polymer material of elasticity its function and reference electrode 9 together builds the micro-fluidic chip
Also include with its property to the strong absorption of ultrasonic wave, ultrasonic wave is absorbed strongly, and thereby at the micro-fluidic chip end
End 17 is to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end 18；And, ultrasonic wave
Damper, its profile of ultrasonic wave damper is in block, tabular or bar-shaped；Its material of the ultrasonic wave damper be microcellular rubber,
Sponge rubber or microporous polyurethane elastomer；And, another elastic clip, another elastic clip is micro- by this with its gripping strength
The sample introduction end 18 of fluidic chip and the ultrasonic wave damper are clipped together bonded to each otherly, and the ultrasonic wave damper is with its material institute
The strong consumption to ultrasonic energy brought, further supports that the terminal 17 arrives the sample introduction end 18 in the micro-fluidic chip
Between limited short distance within realize the rapid decrement of ultrasonic intensity, and thereby further expand the sample introduction end 18 its
Difference between interfacial tension and the terminal 17 its interfacial tension.It is depicted without in legend in the ultrasonic wave damper, legend
Also it is depicted without another elastic clip.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its test liquid
The flow direction of stream.
Miniature ultrasonic transducer units 22 and the higher-order of oscillation that the elastic clip 15 is depicted without in Fig. 1 and is set up thereon
Electric signal transmission cable 23；Also, the higher-order of oscillation electric signal generator and multiple tracks electricity are depicted without in Fig. 1 and Fig. 2
The associate members such as chem workstation.
Involved elastic clip 15 is commercially available.Be available for commercially available alternative spring clamp structure that this case structure is used and moulding and
Size etc., quality is various, and specific elastic clip pattern can be selected as needed.
Involved miniature ultrasonic transducer units 22 are commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal transmission cable 23 is commercially available；Can also be to ultrasonic transducer producer or cable special manufacturer
Family's customization.
Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also be customized to relevant manufacturers.
Each working electrode in this example structure and electrode and reference electrode via each special cable or can be said respectively
Get lines crossed respectively with the corresponding cable interface of the multiple tracks electrochemical workstation as annex or saying interface connection of getting lines crossed.
This case device is that a variety of corresponding aids antibodies are detected with a variety of AIDS antigens, thereby diagnosis of aids.
Claims (10)
1. driving liquid stream is easy to the AIDS diagnosis device of removal with component, the structure of the device includes multichannel micro-fluidic chip,
The structure of the micro-fluidic chip includes installing substrate and cover plate together bonded to each other, the substrate and cover plate be plate object or
Contain the channel structure via mould pressing process or etching technics formation, the base in tablet, that face towards the cover plate of the substrate
Piece also contains and is connected and pierces being formed via mould pressing process, etching technics or simple drilling technology for the substrate with the channel structure
Window structure, substrate bonded to each other being installed together and the cover plate have been built into containing pipeline configuration and phase therewith jointly
The micro-fluidic chip of liquid pool structure even, the locations of structures of the pipeline is located at the substrate and cover plate interface zone bonded to each other,
Its side of the window is blocked by the cover plate and opposite side is opened, and the locations of structures of the window is exactly the locations of structures of the liquid pool, institute
The quantity for stating liquid pool is three, and its locations of structures of two liquid pools therein is located at the sample introduction end of the micro-fluidic chip, remaining one
Its locations of structures of liquid pool is located at the terminal of liquid flowing in its chip when the actual sample introduction of the micro-fluidic chip is tested, and the terminal is entered with this
Sample end is located remotely from each other, one end of the pipeline via be similarly positioned in the manifold shape turnout of the interface zone bonded to each other respectively with positioned at
The Liang Ge liquid pools UNICOM at sample introduction end, this remaining one of the terminal of the other end of the pipeline with being located at the micro-fluidic chip
Liquid pool UNICOM, and, the working electrode that is sequentially respectively installed in the pipeline on diverse location and to electrode and reference electrode,
The working electrode is by conductive electrode and the gold size for having embedded AIDS specific antigen being attached on the conductive electrode
Sensitive membrane is constituted, and the construction of the pipeline is in parallel construction, and the pipeline in parallel construction is made up of four lateral parallel connections,
The quantity of the working electrode is four, and the installation position of four working electrodes is located in four laterals respectively, with
And, the specific antigen in the sensitive membrane structure of its top layer gold size of four working electrodes is respectively can specificity knot to aids antibody
Close four kinds of AIDS antigenic substances, four kinds of antigenic substances be respectively AIDS specific antigen p24, gp41, gp120 and
Gp36, its material of the working electrode is gold material or thermal decomposition conducting polymer material, and its pattern of the working electrode is presented
Sheet is thread, it is characterised in that its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is primary form
Surface, the surface of the primary form its be intended to refer to not by any surface chemical modification or any surface chemical modification
The surface of the primary form of the material, the structure of the device also includes elastic clip, two opposite clamping limb occlusions of the elastic clip
The position of the neighbouring terminal of the micro-fluidic chip is positioned at, fixed installing is attached on a clamping limb at least in
There are miniature ultrasonic transducer units, and, higher-order of oscillation electric signal transmission cable, one end of the higher-order of oscillation electric signal transmission cable
Linked together with the miniature ultrasonic transducer units；The elastic clip facilitates the function that the device is disassembled there is provided one；This is miniature super
Its major function of acoustic wave transducer is that in the test of micro-fluidic chip actual sample introduction, the ultrasonic wave launched using it reduces sample
Interfacial tension between the inwall of solution and the pipeline, can be compatible, also, utilizes the sample introduction end and the end
Difference in the distance between end and the miniature ultrasonic transducer units installation position difference and its ultrasonic intensity experienced,
Difference between its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension, the micro-fluidic chip two ends it
Between interfacial tension difference can form pressure gap between the two ends of the micro-fluidic chip, the pressure gap can drive sample molten
Liquid is to the end flow；Its function of the miniature ultrasonic transducer units is also contained in sample including being checked with its ultrasonic wave launched
Its absorption on the inner surface of pipeline of some large biological molecules, and then check the substrate of the dimethyl silicone polymer material its body
With respect to the effect of swallowing up of the large biological molecule；Its function of the substrate of the dimethyl silicone polymer material includes and cover plate and work electricity
Pole and the micro-fluidic chip is together built to electrode and reference electrode, the base of the dimethyl silicone polymer material that is soft and having elasticity
Piece its function also includes, with its property to the strong absorption of ultrasonic wave, ultrasonic wave being absorbed strongly, and thereby micro-fluidic at this
The chip terminal is to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end；And, ultrasonic wave
Damper, its profile of ultrasonic wave damper is in block, tabular or bar-shaped；Its material of the ultrasonic wave damper be microcellular rubber,
Sponge rubber or microporous polyurethane elastomer；And, another elastic clip, another elastic clip is micro- by this with its gripping strength
The sample introduction end of fluidic chip and the ultrasonic wave damper are clipped together bonded to each otherly, and the ultrasonic wave damper is brought with its material
The strong consumption to ultrasonic energy, further support in the micro-fluidic chip terminal to limited between the sample introduction end
Short distance within realize the rapid decrement of ultrasonic intensity, and thereby further expand described its interfacial tension of sample introduction end with it is described
Difference between its interfacial tension of terminal.
2. driving liquid stream according to claim 1 is easy to the AIDS diagnosis device of removal with component, it is characterised in that
The pipeline and the lateral and the manifold shape turnout are capillary channel.
3. driving liquid stream according to claim 1 is easy to the AIDS diagnosis device of removal with component, it is characterised in that
The thermal decomposition conducting polymer be by polyimides or polyacrylonitrile be heat-treated through anoxybiotic after the conductive material that is formed.
4. driving liquid stream according to claim 1 is easy to the AIDS diagnosis device of removal with component, it is characterised in that
The width or diameter of the working electrode between 0.1 micron to 2000 microns, and, the length of the working electrode is 1
Micron is between 15000 microns.
5. driving liquid stream according to claim 1 is easy to the AIDS diagnosis device of removal with component, it is characterised in that
The thickness of the gold size sensitive membrane is between 10 nanometers and 200 nanometers.
6. driving liquid stream according to claim 1 is easy to the AIDS diagnosis device of removal with component, it is characterised in that
Its material of the cover plate in structure is dimethyl silicone polymer material.
7. driving liquid stream according to claim 1 is easy to the AIDS diagnosis device of removal with component, it is characterised in that
Positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at this remaining one of the micro-fluidic chip terminal
Air line distance between liquid pool is between 3 centimetres and 7 centimetres；The cover plate in structure and substrate its thickness are between 1.0
Between millimeter and 5.0 millimeters.
8. driving liquid stream according to claim 1 is easy to the AIDS diagnosis device of removal with component, it is characterised in that
The microcellular rubber is micropore silicon rubber or micropore fluorubber.
9. driving liquid stream according to claim 1 is easy to the AIDS diagnosis device of removal with component, it is characterised in that
The structure of the device also includes higher-order of oscillation electric signal generator, its other end of the higher-order of oscillation electric signal transmission cable and the height
Frequency vibration swings the connection of electric signal generator.
10. driving liquid stream according to claim 1 is easy to the AIDS diagnosis device of removal with component, it is characterised in that
Its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 5 milliwatts and 5000 milliwatts, and the miniature ultrasonic is changed
The frequency of its ultrasonic wave operationally launched of energy device is between 100KHz and 12MHz.
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| CN201610089762.2A CN107020156A (en) | 2016-02-01 | 2016-02-01 | Driving liquid stream is easy to the AIDS diagnosis device of removal with component |
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| CN201610089762.2A CN107020156A (en) | 2016-02-01 | 2016-02-01 | Driving liquid stream is easy to the AIDS diagnosis device of removal with component |
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| CN110560186A (en) * | 2019-08-28 | 2019-12-13 | 国家纳米科学中心 | method for synthesizing biological membrane nano particles by using micro-fluidic chip and micro-fluidic chip |
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Application publication date: 20170808 |