CN107377017A - Micro flow control chip device using dimethyl silicone polymer as substrate material - Google Patents
Micro flow control chip device using dimethyl silicone polymer as substrate material Download PDFInfo
- Publication number
- CN107377017A CN107377017A CN201610355473.2A CN201610355473A CN107377017A CN 107377017 A CN107377017 A CN 107377017A CN 201610355473 A CN201610355473 A CN 201610355473A CN 107377017 A CN107377017 A CN 107377017A
- Authority
- CN
- China
- Prior art keywords
- micro
- substrate
- silicone polymer
- dimethyl silicone
- pipeline
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
- B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to a kind of micro flow control chip device using dimethyl silicone polymer as substrate material, belong to analysis testing field.The substrate of micro-fluidic chip is made using dimethyl silicone polymer i.e. PDMS that is cheap and easily processing, some problems be present.This case main points are, the selected PDMS with ecosystem surface of substrate, and fastening is positioned at the sample liquid stream terminal of the micro-fluidic chip its neighbor positions manually by the hinge-type fixture for being attached to miniature ultrasonic transducer units, sample liquid stream is induced to be flowed from its sample introduction end of the chip along its pipeline of the chip to the terminal direction with ultrasonic wave, micron silica granule filler is filled up in its pipeline of the chip, its function of the filler includes supporting pipeline inwall, and, with the set of hydrophily fluid channel come the hydrophobic pipeline of compensatory script, and, extruding, cover inner-walls of duct face and thereby reduce the chance that large biological molecule contacts with the internal face.
Description
Technical field
The present invention relates to a kind of micro flow control chip device using dimethyl silicone polymer as substrate material, belongs to analysis and surveys
Examination field.
Background technology
For the microflow control technique overall general picture of itself, famous micro-fluidic expert Mr. Lin Ping Cheng may refer to not
The monograph " diagram Microfluid based Lab on a chip " gone out before long, the monograph is published via Science Press, and the monograph is for miniflow
Past of control technology, now, and, vision of the future etc. etc., suffer from detail, be deep into long opinion of detail
State.
The field that micro-fluidic chip is often applied to include to containing large biological molecule sample solution carry out it is various analysis,
Detection.
The basic framework of micro-fluidic chip, including it is etched with the substrate of conduit and the cover plate to fit together therewith, institute
State the fluid course on substrate, before upper cover plate is assembled, it is apparent on see to be exactly some conduits, wait until lid is covered thereon
After piece, just really closure forms the fluid course, and the conduit inner surface of the conduit is together with the part that surround the conduit
Cover plate forms described fluid course together；So, it is clear that assemble the fluid course after completing, its inner surface face
Long-pending major part is the inner surface area of that conduit, and in other words, the state or property of the conduit inner surface are substantially determined
The integrality or property of the fluid course are determined；Therefore say, the inner surface state or interior of this conduit of the structure on substrate
Surface nature is key factor；In principle, any material that can be kept or keep its solid forms substantially, can be used
To make substrate and cover plate, such as, the material that can act as substrate and cover plate can be monocrystalline silicon piece, quartz plate, sheet glass, height
Polymers such as dimethyl silicone polymer, polymethyl methacrylate, makrolon etc.；Certainly, the selection of substrate and the choosing of cover plate
Material be able to can also be differed with identical；From the point of view of material consumption, manufacture difficulty and application popularization prospect etc. etc., these
Not small difference, the especially selection of that substrate between material be present, have a great influence.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, at this
It is extremely simple that conduit is made on the substrate of sample, and the lower cost for material, substrate is made with the polydimethyl siloxane material,
Conduit is being suppressed or etched thereon, and is being engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes,
It is a kind of more satisfactory selection seemingly；Certainly, patch material can also select to use cheap polydimethyl siloxane material：
So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, and making is extremely simple, seems and should also be as
It is extremely easy to popularize, promotes.
But thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that alphabetical PDMS is referred to of abridging, itself are a kind of strong
Strong hydrophobic material, conduit is built on this material, if without being operated for the modified of the conduit surface, then, it is whole
After body assembling is completed, that is, after covering cover plate, because the conduit its inner surface in structure occupies most fluid course
Inner surface, then, its strong hydrophobic property of the PDMS conduits inner surface, is deciding factor, it can be similar to water
The fine liquid stream of polar liquid of solution by becoming very difficult, its flow resistance is big, or even in general Micropump is all difficult to
Promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar；Therefore, existing
Among having technology, modification is modified particular for the conduit inner surface on the PDMS material, is necessary operation；So, this
The individual modified operation for PDMS conduit inner surfaces is pretty troublesomeThat falls nor this problem, forms serious technical puzzlement,
It is another problem：PDMS polymer molecules inside its body phase of this PDMS material substrate have to be diffused to the surface, moves automatically
The characteristic of shifting, the characteristic that this substrate body phase inside PDMS polymer molecules are diffused to the surface, migrated automatically, will cause by table
State after the modification of its inner surface of that conduit of face modifying and decorating can not maintain the sufficiently long time, and that is through surface
Holding time for conduit its inner surface state after modification is substantially only sufficient to the time needs for completing laboratory internal test experiments；
In other words, by surface is modified or the PDMS conduit inner surfaces of surface modification, after it is modified or institute's shape after modification is said
Into surface state can not be lasting, but soon automatically tend to or say become again surface be modified before surface state, compared with
The strong hydrophobic surface state of that script is returned in the short time, then, just think, such micro-fluidic chip can be big
Amount making, mass storage, it is widely popularized, answer is it is obvious that is, impossible.Conduit on this PDMS material, does not do
If surface modification, it can not pump and pass through similar to the fine liquid stream of polar solvent of the aqueous solution, chip also cannot just use；And such as
Fruit has been cooked surface modification, can not persistently keep its state after modifying again, or equally can not popularization and application.
According to the literature, there is a kind of expedient solution, be that a small amount of surface-active is added in sample to be tested solution
Agent, so as to which during test, dynamic, temporary transient effective surface hydrophilic layer is built temporarily in the PDMS conduits inner surface；
However, due to the amphipathic characteristic of surfactant, the introduced surfactant of the program is inevitable simultaneously also can be with sample solution
In tested composition occur combination so that tested composition can not be detected normally or it is detected ratio and dubiously dropped
It is low；The surfactant is possibly even fully wrapped around by tested composition with micelle form so that tested composition can not be detected or
Identification；Therefore, this kind adds the scheme of surfactant into sample solution, is far from a preferable solution.
So, how to accomplish that substrate can either be made using cheap PDMS material, and the conduit can be released
Inner surface decorating state can not persistently, chip can not largely make, largely lay in and then be widely popularized and such a make this area
The puzzlement that numerous professionals are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, it is stated that, its surface is strongly hydrophobic above, this strong hydrophobic
Material surface and also have another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and
And these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into it is gradually deep, until
It is heavy to be trapped within the body phase of PDMS substrates, in fact, this process, partly it is also due to PDMS material body phase interior polymer
Molecule, which has, to be diffused to the surface, caused by travel motion；Such case, it can also be explained from another angle, i.e. continue not
Disconnectedly from inside PDMS body phases to those polymer molecules of its diffusion into the surface, migration, its result moved, be little by little by that
It has been involved in a bit by the large biological molecule of adsorption within the body phase of PDMS substrates, briefly, these adsorbed biologies
Macromolecular is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up the phenomenon of large biological molecule, its institute
Caused by influence, necessarily cause the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, the problem of PDMS substrates, is, its not only adsorption large biological molecule, and swallow up biological big point
Son, so, as the large biological molecule of experiment test object, its disappearance will not stop because surface saturation is adsorbed, and
It is, it is constantly adsorbed, also constantly swallowed up.
On PDMS substrates, its body phase is constantly swallowed up and tests showing for associated biomolecule macromolecular in related experiment test process
As, it is to say that another kind, which is explained, substantial amounts of Minute pores in PDMS body phases be present, associated biomolecule macromolecular by after adsorption,
Depression enters these Minute pores, and then is swallowed up；However, inventor thinks, those can allow the sky of miniature scale
Qi leel squeezes into the Minute pores therebetween, not equal to saying that they also can directly allow the large biological molecule of relative large scale to enter
Enter, both difference on yardstick are huge, must not make sweeping generalizations.Explanation is bypassed, in any case, as dependence test analysis object
Large biological molecule is adsorbed by PDMS substrate conduits inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective
Existing phenomenon.
, can be from containment PDMS surfaces pair in order to prevent swallow up effect of this PDMS substrate bodies relative to large biological molecule
The absorption of large biological molecule addresses, and method is chemically modified modification aiming at the PDMS material surface, for
For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described channel portion, by chemistry
The conduit inner surface of modification, can contain its absorption to large biological molecule, and then avoid large biological molecule quilt
PDMS substrate body phases are swallowed up；But or that old problem, that is, the chemical modification on PDMS material surface is modified it
Surface state afterwards can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface, migrate automatically
Process, can soon by that by surface chemical modification be modified conduit inner surface state become again script it is strong it is hydrophobic simultaneously
And the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS substrates its
Conduit inner surface is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, and can is enough
Reach and contain the absorption process of the PDMS substrate conduit inner surfaces to large biological molecule for a long time, and then prevent PDMS substrate bodies relative
The effect of swallowing up of large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life,
It is exactly a very intractable problem.The problem equally makes this area numerous specially as another problem addressed above
Industry personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.Should
Also be present many year in problem, so far, also not yet properly settled.
Third, the polydimethyl siloxane material, namely PDMS material, the active force very little wherein between polymer molecule,
The polymer molecule of low polymerization degree inside its body phase is in constantly into the dynamic process of surface migration, therefore, includes PDMS
After it has been formed, the surface topography in its inner-walls of duct face of chip can be because of described low polymerization degree polymer point for the chip of substrate material
Son moves about, pours into the surface and change, and the change of its pattern is somewhat similarly to Rheological Deformation, trickling deformation or creep and become
Pattern caused by shape institute is possible changes；In the higher PDMS material of some low polymerization degree component ratios, this kind somewhat similar
Can be showed in the phenomenon of Rheological Deformation, trickling deformation or the deformation of creep must be than more significant；This kind include PDMS substrates chip its
The dynamic of inner-walls of duct surface topography after shaping changes property, namely the described property or stream that are somewhat similarly to Rheological Deformation
Dropping down the property of deformation or the property of the deformation of creep, the word of rheology one is applied mechanically in this case, integration, also referred to as the rheological equationm of state or rheology become
Shape property, the dynamic of corresponding inner-walls of duct face pattern change, and this case is also referred to as rheology；As described above, because of this kind of material
Expect certain Rheological Deformation property as described above that its own has, its manufactured goods actually can at leisure Rheological Deformation and cause
The manufactured goods total form generation changes；Influence caused by its Rheological Deformation property, it is to be not easy to feel in terms of macroscopic perspective
Examine, still, for the very fine micro-fluidic chip of its internal structure, the rheological equationm of state can but cause bigger shadow
Ring, the fluid course namely the pipeline therefore, during pre-production formed, because the wall of its tube chamber of pipeline is main
It is the wall of PDMS materials, so, the cross-sectional area of the pipeline actually can gradually become because of rheology over time
It is small, stated differently, since the reason for the rheology, the natively finer pipeline that is flowed through for liquid sample
Its tube chamber the more can become the more narrow, in the case of flow time is sufficiently long, or even the pipeline tube chamber can partly close or all
Ground closes, and then make it that sample fluid course is thoroughly blocked, and closes with being even also not reaching to its luminal part of pipeline
The degree closed or fully closed, its fluid passage that can be provided of that pipeline narrow because of rheology, then, in pipeline
Its inner surface originally be exactly strongly it is hydrophobic in the case of, aqueous sample liquid is just more difficult to by becoming more so because of rheology
Narrow micro-channel, then, the influence of substrate rheology how is resisted, maintains the basic framework of the pipeline not occur seriously to become for a long time
The problem of shape, also, keep the long-term unobstructed of the pipeline is exactly a urgent need to resolve.
The content of the invention
The technical problem to be solved by the invention is to provide a package solution, while solve to address above
A series of problems.
The present invention solves the technical problem by following scheme, and the device that the program provides is one kind with poly dimethyl silicon
Micro flow control chip device of the oxygen alkane as substrate material, the structure of the device include micro-fluidic chip, the knot of the micro-fluidic chip
Structure includes being bonded to each other the substrate and cover plate of installing together, and its test liquid of the micro-fluidic chip flows into sample end and the sample liquid stream
The terminal of flowing is located remotely from each other, and between 3 centimetres and 10 centimetres, emphasis is to be somebody's turn to do for the distance between the sample introduction end and the terminal
Its material of substrate is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the surface of the primary form
It is intended to refer to not by any surface chemical modification or the table of the primary form of the material of any surface chemical modification
Face, the structure of the device also include hinge-type fixture, the hinge-type fixture its appearance profile likeness in form hinge, the hinge-type fixture by
Two hinges being mutually hinged and through a fastening screw of two hinges and one and the fastening screw
The nut of fastening and hand break loose manually that is used for for matching and being connected together with the fastening screw is formed, the hinge-type fixture
Two hinges are respectively mutually drawn close with its tip and clamp the micro-fluidic chip, and the tip is positioned at the neighbouring of the micro-fluidic chip
Fixation, which is attached, in the locations of structures of the terminal, in a hinge at least in is equiped with miniature ultrasonic transducing
Device, and, higher-order of oscillation electric signal transmission cable, one end and the miniature ultrasonic of the higher-order of oscillation electric signal transmission cable change
Energy device links together；The hinge-type fixture provides a function of facilitating the device to disassemble；The miniature ultrasonic transducer units
Its major function is in the actual sample introduction test of micro-fluidic chip, utilizes the sample introduction end and the terminal and the miniature ultrasonic
Difference in the distance between wave transducer installation position difference and its ultrasonic intensity experienced, described in induced synthesis
Difference between its interfacial tension of sample introduction end and the terminal its interfacial tension, the interface between the micro-fluidic chip both ends
Power difference can form pressure gap between the both ends of the micro-fluidic chip, and the pressure gap can drive sample solution to described
End flow；It is soft and have the substrate of the dimethyl silicone polymer material of elasticity its function and include with it to the strong suction of ultrasonic wave
The property of receipts, ultrasonic wave is absorbed strongly, and thereby in the micro-fluidic chip terminal to limited between the sample introduction end
Short distance within realize the rapid decrement of ultrasonic intensity；And many silica dioxide granules, many silica
Particles filled its tube chamber of the pipeline is filled up by many silica dioxide granules in the pipeline of the micro-fluidic chip, should
For its particle size range of silica dioxide granule between 10 microns and 200 microns, the silica dioxide granule is silica crystalline state
Grain, silica glass granule or silica composition its inorganic glass particles of shared percentage by weight more than 80%.
Its particle diameter of the silica dioxide granule can be that the basis between 10 microns and 200 microns is actually needed and any
The particle diameter specified, such as 10 microns, 30 microns, 70 microns, 100 microns, 150 microns or 200 microns of the particle diameter, etc..
The scope of further preferred its particle diameter of the silica dioxide granule is between 100 microns and 200 microns.
Its pattern of the silica dioxide granule is unlimited, and the silica dioxide granule for example can be spheric granules, rod-shaped particles, length
Cube shape particle or any amorphous granular.
The various silica dioxide granules manufacturing technology of itself is known technology.
Various forms, the silica dioxide granule market of all size are on sale.
The silica dioxide granule can also customize to specialized factory.
The preferred scope of its internal diameter of pipeline is between 500 microns and 1000 microns；It is but excellent compared to above-mentioned
The internal diameter of the pipeline scope of choosing, more tiny internal diameter of the pipeline or thicker internal diameter of the pipeline are also what this case was allowed.
Described miniature ultrasonic transducer units can certainly be all installed in two hinges；But only install one
Individual miniature ultrasonic transducer units are dealt with enough to be used.
The word of hinge one art-recognized meanings of itself are known.
Only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field,
It is known.
Various sizes, variously-shaped ultrasonic transducer are commercially available；Its size of commercially available miniature ultrasonic transducer units
It may diminish to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface its
It is known general technology for the professional in ultrasonic technology field for body.This case is not to this expansion superfluous words.
Only with regard to naked PDMS substrates itself conduit molding or lithographic technique for, be open-and-shut known technology；
Similarly, the technology of hole-opening is even more known simple technique on naked PDMS substrates.This case is not also to this expansion superfluous words.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the device can also include higher-order of oscillation electric signal generator；The higher-order of oscillation electric signal transmission cable
Its other end can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, come for the professional in ultrasonic technology field
Say, be simple and known；The higher-order of oscillation electric signal generator can customize to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 2 milliwatts and 9000 milliwatts
Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with
Between 12MHz.
This case device can further include some annexes certainly, and the annex can for example coordinate with micro-fluidic chip
The electrically or optically detection device used.The equipment that the electricity, optics etc. are used cooperatively with micro-fluidic chip, may refer to
The monograph.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, such as：Polypropylene,
Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, for example do
Into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and realized in the extremely short distance to ultrasonic wave
Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip
Using dimethyl silicone polymer it is used as the cover plate, and this case technical scheme is allowed.
Described its thickness of cover plate and substrate can allow be any setting the thickness for being easy to assembling, the thickness of recommendation or say
Preferable thickness is between 1.0 millimeters and 5.0 millimeters.Less thickness is advantageous to save material.
It is an advantage of the invention that its close position positions the hinge-type folder in the terminal of the micro-fluidic chip
Tool, installation miniature ultrasonic transducer units are attached in the hinge-type fixture its hinge, are launched with the miniature ultrasonic transducer units low
The ultrasonic wave of power, high-frequency band, meanwhile, using its strong absorbability to ultrasonic wave of dimethyl silicone polymer substrate,
In shorter distance, it is, in from the terminal to the very short distance of only several centimeters yardsticks the sample introduction end,
Reach the rapid decrement of ultrasonic intensity, the difference of the interfacial tension thereby caused at the both ends of the micro-fluidic chip,
And then using the pressure gap between its both ends for being formed of the difference of the interfacial tension between the both ends, drive test liquid
Stream flows in the pipeline to the terminal direction.By this case liquid stream drive scheme, altogether dispense with traditional
The equipment of Micropump etc；The micro-fluidic chip of Micropump is eliminated, its structure is more succinct, and its production process is less, its cost of manufacture
Lower, this kind of succinct structure is more beneficial for the low cost application for reaching micro-fluidic chip.
Its scheme of this case and by the pipeline except electrode installation take up space in addition to all clearance spaces whole
Filled up with the silica dioxide granule.Those are filled in the silica dioxide granule in pipeline, and this case is to its overall abbreviation titanium dioxide
Silicon grain filler, its function include support, withstand the tube chamber of the pipeline its inwall so that the tube chamber of the pipeline is from because of institute
State rheology and narrow, prevent its reduced cross-sectional area of the tube chamber of the pipeline or even close, so as to maintain the base of the pipeline for a long time
Serious deformation does not occur for this framework.
Numerous silica dioxide granule entities are mutually banked up under this case framework together at random in the pipeline, the titanium dioxide
The function of silicon grain filler, also include certainly with the hydrophilic surface of its silica dioxide granule, utilize each adjacent titanium dioxide
Mutual formed complications are continuous and its inner surface that is mutually close together by water-wetted surface of silicon grain has hydrophilic
The space of property, in the hydrophilic fluid channel of the pipeline Inner Constitution network morphology, although this complications can be continuous
All the time the fluid channel with hydrophilic nmature, then, inside the tube chamber of the pipeline simultaneously and a plurality of miniflow deposited
The effect of its synthesis of road, integrated, cumulative superposition in other words, is the equal of the relatively fine hydrophilic capillary of caliber
Passage；Its presence of the continuous hydrophilic fluid channel of the complications, the PDMS of the primary form is counteracted to a large extent
The flow resistance for liquid sample caused by the strong hydrophobic property in its surface of substrate；In other words, because of liquid sample, it is accounted for
The composition of maximum ratio is water, and liquid sample is substantially exactly the aqueous solution, therefore, the presence of the silica dioxide granule filler,
Can significantly overcome with the unmodified PDMS substrates of hydrophobic property and surface its to the aqueous solution it is incompatible, repel and
Barrier effect, so be greatly reduced belong to properties of Aqueous Solution sample liquid stream its be advanced through the resistance of pipeline.
Under this case framework, inside the tube chamber of the pipeline, the area of its lumen wall wall of former pipeline, because of described two
The rigid of silicon oxide particle filler ties up, the table of part hydrophobic property that still can also be exposed on lumen wall wall
Its area of face is already dramatically reduced, and the area on its still exposed part surface for belonging to hydrophobic property is much
Less than the wall surface area of the hydrophobic property of the lumen wall wall in the presence of no silica dioxide granule filler；
So, under this case framework, in its lumen of the pipeline, its remaining hydrophobic surface increases surface newly with hydrophilic part, and it is folded
Adding, comprehensive, summation, cumulative technique effect, is to form the surface for tending to be hydrophilic on the whole；In other words, in institute
State inside the tube chamber of pipeline, its interfacial tension between sample solution of the part surface of remaining hydrophobic property, plus parent
The part of aqueous nature increases its interfacial tension between sample solution of surface newly, that it is superimposed, comprehensive, summation, cumulative
Technique effect, be so that its solid-liquid interfacial tension inside the tube chamber of the pipeline synergy level off to capillary glass tube its
Interfacial tension between tube chamber inner surface and sample solution.
Therefore, exist in the pipeline under this case framework for having stated silica dioxide granule filler, while rely on this
Case sample liquid stream drive scheme, it becomes possible to the driving to sample liquid stream is reached with relatively low ultrasonic power, with this case mechanism
The test liquid is driven to flow to the end flow.
On the other hand, due to its rheological equationms of state of the PDMS as substrate material, it is filled in the pipeline
The interior silica dioxide granule filler, can gradually by constantly Rheological Deformation and to arest neighbors free space promote PDMS
The pipeline wall of material more tightly wraps up, and this process eventually causes those part two for pressing close to the lumen wall wall
Silicon oxide particle is stuck in original place or is stuck in original place or embedding in-situ silica dioxide granule and its embedded in original place, the part
Incarceration is together, described due to this reason each other for the remaining silica dioxide granule banked up with being mutually closely packed together
Silica dioxide granule filler just will not move in the pipeline easily, silica dioxide granule therein be also substantially all by
Original place is locked in, therefore the stream framework of the micro-fluidic chip can be kept for a long time.
The particle size range of its particle of this case silica dioxide granule filler between 10 microns and 200 microns, compared to
For general organic molecule, general large biological molecule, the particle diameter of its particle of this case silica dioxide granule filler can be rated as huge
Greatly, due to its huge particle diameter, and the inner surface of the surface of its polarity and the pipeline of strong hydrophobic PDMS materials can not
Mutually perhaps say and mutually melt, then, the huge silica dioxide granule of this kind of particle diameter can not be by the pipeline of PDMS materials
Surface is adsorbed, and can not more be swallowed up by the inner surface of the pipeline of PDMS materials, the huge titanium dioxide of this kind of particle diameter
Silicon grain surely not be fully sunk in easily or sink to completely PDMS materials its among intrinsic many Minute pores, and
Because above it is stated that the rheology the reason for, the silica dioxide granule filler can be stuck in original place in other words it is embedding
In original place without moving easily.
Inside the tube chamber of the pipeline, its lumen wall wall of former pipeline is entirely the surface of hydrophobic property originally, such as
Upper described, under this case framework, rigid because of the silica dioxide granule filler ties up, on lumen wall wall still also
Its area of the surface of part hydrophobic property that can be exposed is already dramatically reduced, and it is still exposed to belong to hydrophobicity
The area on the part surface of matter has been far smaller than the tube chamber in the presence of no silica dioxide granule filler
The wall surface area of the hydrophobic property of inwall wall；The reason for because of the rheology, it is close to those dioxies of lumen wall wall
Silicon carbide particle, little by little it is partially embedded into or is partly absorbed among the lumen wall wall, the dioxy among the state
Silicon carbide particle its towards the part surface of lumen wall wall with have occurred and that matching deformation the lumen wall wall it is tight
Closely connected conjunction, then, the matching is deformed and closely pasted with silica particles having occurred and that in lumen wall wall
The part wall of conjunction actually can not contact with flowing through the sample solution of pipeline, that is to say, that the wall of this part is
Through suction-operated can not be produced to the large biological molecule therein that flows through the sample solution of pipeline and organic molecule.Based on above-mentioned
Mechanism, then because fill up the pipeline the silica dioxide granule filler its presence, its lumen wall wall of the pipeline
The overwhelming majority in face is tightly covered, and these described walls tightly covered can not be with flowing through the pipeline
Sample solution contact, only remaining fraction be not brought into close contact by its surface of silica dioxide granule, tightly cover
Hydrophobic surface may be contacted with sample solution, in other words, under this case framework, large biological molecule in sample solution and have
Its direct touch opportunity with hydrophobic PDMS walls of machine molecule is significantly reduced, thus, the lumen wall faces of PDMS materials its
The adsorptive hindrance and interference of swallowing up of large biological molecule and organic molecule are greatly reduced；As described above, this case scheme
Be advantageous to exclude or weaken the adsorptive hindrance and swallow up interference, be advantageous to be lifted the reliability of correlation analysis test data.
As described above, this case silica dioxide granule filler, its function actually includes being similar to being directed to PDMS materials
Inner surface of pipeline carry out chemical modification effect, functioning as its this respect of this case is by the pipeline of the PDMS materials
Surface by the surface modification of hydrophobic property into the surface of hydrophilic nmature, still, with general, usual PDMS surface chemical modifications
Its hydrophilic chemical decorative layer retention time is short, hydrophilic effect can not keep situation prolonged enough different, particle described in this case
Huge silica dioxide granule filler, its particle being stuck by rheology can not both be moved easily, its huge particle diameter
Particle can not more be swallowed up easily by the inner-walls of duct of PDMS materials, and therefore, its from this respect is similar to surface chemical modification
Technique effect on see, its technique effect of this case, be form it is permanent, can not erase, can not consume, not be corroded, not by
Hydrophilic surface reforming layer swallowing up, being not dissolved, it is just comparable to one kind in its correlation table of PDMS base materials in effect
The permanent hydrophilic modifying superficial layer built on face.
It is related that the technical scheme of this case has dissolved its application to dimethyl silicone polymer substrate addressed above totally
A series of technical barriers.Based on this case scheme, the very cheap polydimethyl siloxane material of this kind is just possible to micro- at this
It is prepared by fluidic chip, production, using etc. field play bigger effect.
The miniature ultrasonic transducer units have been installed in fixation in the hinge-type fixture its hinge in this case structure, the structure
A function of facilitating the device to disassemble is provided, in this way, the hinge-type fixture is together with miniature ultrasonic appended in its hinge
Transducer just easily can be mutually disengaged with the micro-fluidic chip, then, the component that the part can freely depart from just can be good
Property is re-used cyclically many times；The architectural feature is advantageous to save the use cost of the device.
Brief description of the drawings
Fig. 1 is its rough outside side view of this case micro flow control chip device.
In figure, 1 is hinge-type fixture, and 2 sign linkwork positions, 3 be fastening screw, and 4 be higher-order of oscillation telecommunication
Number transmission cable, 5 be miniature ultrasonic transducer units, and 6,11 be two different hinges of locations of structures respectively, and 7 be cover plate, and 8 be base
Piece, 9 be sample introduction end, and 10 be terminal, and 12 be nut；The hinge-type clamp structure in legend is only the legend structure of signal, real
Border its structure of the hinge-type fixture is not limited to the legend hinge-type clamp structure；Arrow in legend indicates the micro-fluidic core
Piece its in actual motion, by pressure at two ends difference drive, the flow direction of its sample liquid stream.
Embodiment
In this case that Fig. 1 is shown embodiment, the structure of the device includes micro-fluidic chip, the micro-fluidic chip
Structure includes being bonded to each other the substrate 8 and cover plate 7 of installing together, and its test liquid of the micro-fluidic chip flows into sample end 9 and the examination
The terminal 10 of sample fluid flow is located remotely from each other, the distance between the sample introduction end 9 and the terminal 10 between 3 centimetres with 10 centimetres it
Between, emphasis is, its material of the substrate 8 is dimethyl silicone polymer material, and its surface of substrate 8 is the surface of primary form, should
The surface of primary form its be intended to refer to not by the material of any surface chemical modification or any surface chemical modification
Primary form surface, the structure of the device also includes hinge-type fixture 1, and its appearance profile likeness in form of hinge-type fixture 1 is closed
Page, the hinge-type fixture 1 are tight by two hinges 6,11 for being mutually hinged and through one of two hinges 6,11
Gu screw 3 and one match with the fastening screw 3 and are used to fasten manually and manually with what the fastening screw 3 connected together
The nut 12 released is formed, and the nut 12 employs in this example is easier manually operated butterfly nut, butterfly nut city
Field is on sale, and two hinges 6,11 of the hinge-type fixture 1 are respectively mutually drawn close with its tip and clamp the micro-fluidic chip, the end
The tip is positioned in the locations of structures of the neighbouring terminal 10 of the micro-fluidic chip, is pasted in a hinge at least in
Attached fixation is equiped with miniature ultrasonic transducer units, and in this example, the miniature ultrasonic transducer units 5 are to attach to be fixed in hinge 6, with
And higher-order of oscillation electric signal transmission cable 4, one end of the higher-order of oscillation electric signal transmission cable 4 and the miniature ultrasonic transducing
Device 5 links together；The hinge-type fixture 1 provides a function of facilitating the device to disassemble；The miniature ultrasonic transducer units 5
Its major function be in the test of micro-fluidic chip actual sample introduction, it is miniature with this using the sample introduction end 9 and the terminal 10
Difference in the distance between the installation position of ultrasonic transducer 5 difference and its ultrasonic intensity experienced, induce shape
Difference between described its interfacial tension of sample introduction end 9 and the terminal 10 its interfacial tension, the micro-fluidic chip both ends 9,
Interfacial tension difference between 10 can form pressure gap between the both ends 9,10 of the micro-fluidic chip, the pressure gap meeting
Driving sample solution flows to the direction of terminal 10；The substrate 8 of the dimethyl silicone polymer material that is soft and having elasticity its
Function includes, with its property to the strong absorption of ultrasonic wave, absorbing ultrasonic wave strongly, and thereby in the micro-fluidic chip
The terminal 10 is to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end 9；It is and many
Silica dioxide granule, many silica dioxide granules are filled in the pipeline of the micro-fluidic chip, its tube chamber quilt of the pipeline
Many silica dioxide granules are filled up, and its particle size range of the silica dioxide granule, should between 10 microns and 200 microns
Silica dioxide granule is silica crystalline particles, silica glass granule or silica composition its shared percentage by weight
Inorganic glass particles more than 80%.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its try
The flow direction of sample liquid stream.
Fig. 1 is depicted without the associate members such as the higher-order of oscillation electric signal generator.
Described its main body of hinge-type fixture can simply be changed a social system using commercially available hinge；Can also be to hardware processing factory
Customization.
Involved miniature ultrasonic transducer units 5 are commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal transmission cable 4 is commercially available；Can also be special to ultrasonic transducer producer or cable
Industry producer customizes.
Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also determine to relevant manufacturers
System.
Claims (6)
1. using dimethyl silicone polymer as the micro flow control chip device of substrate material, the structure of the device includes micro-fluidic core
Piece, the structure of the micro-fluidic chip include being bonded to each other the substrate and cover plate of installing together, its test liquid of the micro-fluidic chip
Flow into sample end and the terminal of the sample fluid flow be located remotely from each other, the distance between the sample introduction end and the terminal between 3 centimetres with
Between 10 centimetres, it is characterised in that its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is primary form
Surface, the surface of the primary form its be intended to refer to and do not pass through any surface chemical modification or any surface chemical modification
The material primary form surface, the structure of the device also includes hinge-type fixture, its appearance profile of the hinge-type fixture
Hinge is similar to, the hinge-type fixture is by two hinges being mutually hinged and a fastening spiral shell through two hinges
Silk and one matchs with the fastening screw and are used for manual fastening and hand break loose with what the fastening screw connected together
Nut is formed, and two hinges of the hinge-type fixture are respectively mutually drawn close with its tip and clamp the micro-fluidic chip, the tip
It is positioned in the locations of structures of the neighbouring terminal of the micro-fluidic chip, is attached in a hinge at least in solid
Surely miniature ultrasonic transducer units are equiped with, and, higher-order of oscillation electric signal transmission cable, the higher-order of oscillation electric signal transmission cable
One end linked together with the miniature ultrasonic transducer units；The hinge-type fixture provides the work(for facilitating the device to disassemble
Energy；Its major function of the miniature ultrasonic transducer units be in the test of micro-fluidic chip actual sample introduction, using the sample introduction end with
And the distance between the terminal and the miniature ultrasonic transducer units installation position difference and its ultrasonic wave experienced it is strong
Difference between difference on degree, its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension, the miniflow
Interfacial tension difference between the control chip both ends can form pressure gap between the both ends of the micro-fluidic chip, the pressure
Difference can drive sample solution to the end flow；It is soft and have the substrate of the dimethyl silicone polymer material of elasticity its work(
It can include with its property to the strong absorption of ultrasonic wave, ultrasonic wave is absorbed strongly, and thereby should in the micro-fluidic chip
Terminal is to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end；And many dioxies
Silicon carbide particle, many silica dioxide granules are filled in the pipeline of the micro-fluidic chip, and its tube chamber of the pipeline is permitted by this
More silica dioxide granules are filled up, and its particle size range of the silica dioxide granule is between 10 microns and 200 microns, the dioxy
Silicon carbide particle is that silica crystalline particles, silica glass granule or silica composition its shared percentage by weight exists
More than 80% inorganic glass particles.
2. the micro flow control chip device according to claim 1 using dimethyl silicone polymer as substrate material, its feature
It is, its material of the cover plate is dimethyl silicone polymer material.
3. the micro flow control chip device according to claim 1 using dimethyl silicone polymer as substrate material, its feature
It is, described its thickness of cover plate and substrate is between 1.0 millimeters and 5.0 millimeters.
4. the micro flow control chip device according to claim 1 using dimethyl silicone polymer as substrate material, its feature
It is, the structure of the device also includes higher-order of oscillation electric signal generator, and its is another for the higher-order of oscillation electric signal transmission cable
End is connected with the higher-order of oscillation electric signal generator.
5. the micro flow control chip device according to claim 1 using dimethyl silicone polymer as substrate material, its feature
It is, its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 2 milliwatts and 9000 milliwatts, the miniature ultrasonic
The frequency of its ultrasonic wave operationally launched of wave transducer is between 100KHz and 12MHz.
6. the micro flow control chip device according to claim 1 using dimethyl silicone polymer as substrate material, its feature
Be, its particle size range of the silica dioxide granule between 100 microns and 200 microns, its inside diameter ranges of the pipeline between
Between 500 microns and 1000 microns.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610355473.2A CN107377017A (en) | 2016-05-17 | 2016-05-17 | Micro flow control chip device using dimethyl silicone polymer as substrate material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610355473.2A CN107377017A (en) | 2016-05-17 | 2016-05-17 | Micro flow control chip device using dimethyl silicone polymer as substrate material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107377017A true CN107377017A (en) | 2017-11-24 |
Family
ID=60338145
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610355473.2A Withdrawn CN107377017A (en) | 2016-05-17 | 2016-05-17 | Micro flow control chip device using dimethyl silicone polymer as substrate material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107377017A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
| CN102645429A (en) * | 2012-04-23 | 2012-08-22 | 宁波大学 | Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device |
| CN102645426A (en) * | 2012-03-08 | 2012-08-22 | 宁波大学 | Easy-to-assemble electrogenerated chemiluminescence (ECL) glassy carbon electrode containing high-frequency oscillation mechanism |
| CN103285949A (en) * | 2013-05-27 | 2013-09-11 | 苏州扬清芯片科技有限公司 | Micro-fluidic serum extracting chip |
-
2016
- 2016-05-17 CN CN201610355473.2A patent/CN107377017A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
| CN102645426A (en) * | 2012-03-08 | 2012-08-22 | 宁波大学 | Easy-to-assemble electrogenerated chemiluminescence (ECL) glassy carbon electrode containing high-frequency oscillation mechanism |
| CN102645429A (en) * | 2012-04-23 | 2012-08-22 | 宁波大学 | Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device |
| CN103285949A (en) * | 2013-05-27 | 2013-09-11 | 苏州扬清芯片科技有限公司 | Micro-fluidic serum extracting chip |
Non-Patent Citations (1)
| Title |
|---|
| JAMES FRIEND等: "Microscale acoustofluidics--Microfluidics driven via acoustics and ultrasonics", 《REVIEWS OF MODERN PHYSICS》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107754917A (en) | A kind of micro flow control chip device for taking new fluid type of drive | |
| CN107377017A (en) | Micro flow control chip device using dimethyl silicone polymer as substrate material | |
| CN107754915A (en) | Interfacial characteristics is relied on to carry out the cheap micro fluidic device of cholera diagnosis of liquid stream driving | |
| CN107754904A (en) | Micro flow control chip device using dimethyl silicone polymer as substrate material | |
| CN107754874A (en) | The strong hydrophobic controller used in syphilis diagnosis Multichannel device of its substrate of micro-fluidic chip | |
| CN107457012A (en) | For the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter | |
| CN107519950A (en) | A kind of micro flow control chip device for taking new fluid type of drive | |
| CN107754903A (en) | Interphase interface characteristic is relied on to reach the micro flow control chip device of sample sap flow process | |
| CN107349981A (en) | A kind of micro flow control chip device for taking new fluid type of drive | |
| CN107552105A (en) | Micro flow control chip device using dimethyl silicone polymer as substrate material | |
| CN107349978A (en) | Additional driving liquid stream is easy to the hypotype swine flu detection means of removal with component | |
| CN107552113A (en) | A kind of substrate material is PDMS cholera diagnosis micro flow control chip device | |
| CN107754878A (en) | Using the controller used in syphilis diagnosis of interfacial characteristics transmission liquid stream with cheap micro fluidic device | |
| CN107754909A (en) | The special cheap AIDS diagnosis micro fluidic device of fluid type of drive | |
| CN107344125A (en) | The special cheap AIDS diagnosis micro fluidic device of fluid type of drive | |
| CN107754906A (en) | Strong hypotype swine flu detection chip apparatus of the hydrophobic PDMS as substrate material | |
| CN107486258A (en) | Couple the micro flow control chip device for including hydrophobic substrate of dual drive pattern | |
| CN107552104A (en) | The micro flow control chip device containing hydrophobic substrate of two kinds of type of drive coupling runnings | |
| CN107344127A (en) | Interphase interface characteristic is relied on to reach the micro flow control chip device of sample sap flow process | |
| CN107335484A (en) | The firmly modified micro flow control chip device of its transmission pipeline of sample liquid stream | |
| CN107377012A (en) | Substrate material is PDMS cholera diagnosis multi-channel chip device | |
| CN107754877A (en) | Substrate material is PDMS cholera diagnosis multi-channel chip device | |
| CN107754902A (en) | Its substrate of chip uses the cholera diagnosis micro fluidic device of strong hydrophobic material | |
| CN107754887A (en) | Using the micro flow control chip device of PDMS material substrates | |
| CN107754899A (en) | Selected strong controller used in syphilis diagnosis chip apparatus of the hydrophobic PDMS as substrate material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20171124 |