CN107349978A - Additional driving liquid stream is easy to the hypotype swine flu detection means of removal with component - Google Patents
Additional driving liquid stream is easy to the hypotype swine flu detection means of removal with component Download PDFInfo
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- CN107349978A CN107349978A CN201610329080.4A CN201610329080A CN107349978A CN 107349978 A CN107349978 A CN 107349978A CN 201610329080 A CN201610329080 A CN 201610329080A CN 107349978 A CN107349978 A CN 107349978A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
- B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
Abstract
It is easy to the hypotype swine flu detection means of removal with component the present invention relates to a kind of additional driving liquid stream, belongs to analysis testing field.The substrate physical presence series problem of hypotype swine flu diagnosis micro-fluidic chip is made using dimethyl silicone polymer i.e. PDMS that is cheap and easily processing.This case main points are, the selected PDMS with ecosystem surface of substrate, and by the elastic clip that miniature ultrasonic transducer units are attached on its clamping limb with elastic force snap-in location in its neighbor positions of the sample liquid stream terminal of the micro-fluidic chip, sample liquid stream is induced to be flowed from its sample introduction end of the chip along its pipeline of the chip to the terminal direction with ultrasonic wave, micron silica granule filler is filled up in its pipeline of the chip, its function of the filler includes supporting pipeline inwall, and, with the set of hydrophily fluid channel come the hydrophobic pipeline of compensatory script, and, extruding, cover inner-walls of duct face and thereby reduce the chance that large biological molecule contacts with the internal face.
Description
Technical field
It is easy to the hypotype swine flu detection means of removal, the device with component the present invention relates to a kind of additional driving liquid stream
It is that the special purpose device of hypotype swine flu antigen is diagnosed based on antigen/antibody specific reaction, belongs to analysis testing field.
Background technology
Multichannel micro-fluidic hypotype swine flu diagnostic techniques background, may refer to the patents of invention such as CN201110311127.1
Application case.
Only for the microflow control technique overall general picture of itself, famous micro-fluidic expert Mr. Lin Ping Cheng may refer to not
The monograph " diagram Microfluid based Lab on a chip " gone out before long, the monograph is published via Science Press, and the monograph is to micro-fluidic
Past of technology, now, and, vision of the future etc. etc., suffer from detail, be deep into long discussion of detail.
So, the Important Problems of this case that to have a talk below concern.
The basic framework of micro-fluidic chip, including it is etched with the substrate of conduit and the cover plate to fit together therewith, institute
State the fluid course on substrate, before upper cover plate is assembled, it is apparent on see to be exactly some conduits, wait until lid is covered thereon
After piece, just really closure forms the fluid course, and the conduit inner surface of the conduit is together with the part that surround the conduit
Cover plate forms described fluid course together；So, it is clear that assemble the fluid course after completing, its inner surface face
Long-pending major part is the inner surface area of that conduit, and in other words, the state or property of the conduit inner surface are substantially determined
The integrality or property of the fluid course are determined；Therefore say, the inner surface state or interior of this conduit of the structure on substrate
Surface nature is key factor；In principle, any material that can be kept or keep its solid forms substantially, can be used
To make substrate and cover plate, such as, the material that can act as substrate and cover plate can be monocrystalline silicon piece, quartz plate, sheet glass, height
Polymers such as dimethyl silicone polymer, polymethyl methacrylate, makrolon etc.；Certainly, the selection of substrate and the choosing of cover plate
Material be able to can also be differed with identical；From the point of view of material consumption, manufacture difficulty and application popularization prospect etc. etc., these
Not small difference, the especially selection of that substrate between material be present, have a great influence.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, at this
It is extremely simple that conduit is made on the substrate of sample, and the lower cost for material, substrate is made with the polydimethyl siloxane material,
Conduit is being suppressed or etched thereon, and is being engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes,
It is a kind of more satisfactory selection seemingly；Certainly, patch material can also select to use cheap polydimethyl siloxane material：
So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, and making is extremely simple, seems and should also be as
It is extremely easy to popularize, promotes.
But thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that alphabetical PDMS is referred to of abridging, itself are a kind of strong
Strong hydrophobic material, conduit is built on this material, if without being operated for the modified of the conduit surface, then, it is whole
After body assembling is completed, that is, after covering cover plate, because the conduit its inner surface in structure occupies most fluid course
Inner surface, then, its strong hydrophobic property of the PDMS conduits inner surface, is deciding factor, it can be similar to water
The fine liquid stream of polar liquid of solution by becoming very difficult, its flow resistance is big, or even in general Micropump is all difficult to
Promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar；Therefore, existing
Among having technology, modification is modified particular for the conduit inner surface on the PDMS material, is necessary operation；So, this
The individual modified operation for PDMS conduit inner surfaces is pretty troublesomeThat falls nor this problem, forms serious technical puzzlement,
It is another problem：PDMS polymer molecules inside its body phase of this PDMS material substrate have to be diffused to the surface, moves automatically
The characteristic of shifting, the characteristic that this substrate body phase inside PDMS polymer molecules are diffused to the surface, migrated automatically, will cause by table
State after the modification of its inner surface of that conduit of face modifying and decorating can not maintain the sufficiently long time, and that is through surface
Holding time for conduit its inner surface state after modification is substantially only sufficient to the time needs for completing laboratory internal test experiments；
In other words, by surface is modified or the PDMS conduit inner surfaces of surface modification, after it is modified or institute's shape after modification is said
Into surface state can not be lasting, but soon automatically tend to or say become again surface be modified before surface state, compared with
The strong hydrophobic surface state of that script is returned in the short time, then, just think, such micro-fluidic chip can be big
Amount making, mass storage, it is widely popularized, answer is it is obvious that is, impossible.Conduit on this PDMS material, does not do
If surface modification, it can not pump and pass through similar to the fine liquid stream of polar solvent of the aqueous solution, chip also cannot just use；And such as
Fruit has been cooked surface modification, can not persistently keep its state after modifying again, or equally can not popularization and application.
According to the literature, there is a kind of expedient solution, be that a small amount of surface-active is added in sample to be tested solution
Agent, so as to which during test, dynamic, temporary transient effective surface hydrophilic layer is built temporarily in the PDMS conduits inner surface；
However, due to the amphipathic characteristic of surfactant, the introduced surfactant of the program is inevitable simultaneously also can be with sample solution
In tested composition occur combination so that tested composition can not be detected normally or it is detected ratio and dubiously dropped
It is low；The surfactant is possibly even fully wrapped around by tested composition with micelle form so that tested composition can not be detected or
Identification；Therefore, this kind adds the scheme of surfactant into sample solution, is far from a preferable solution.
So, how to accomplish that substrate can either be made using cheap PDMS material, and the conduit can be released
Inner surface decorating state can not persistently, chip can not largely make, largely lay in and then be widely popularized and such a make this area
The puzzlement that numerous professionals are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, it is stated that, its surface is strongly hydrophobic above, this strong hydrophobic
Material surface and also have another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and
And these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into it is gradually deep, until
It is heavy to be trapped within the body phase of PDMS substrates, in fact, this process, partly it is also due to PDMS material body phase interior polymer
Molecule, which has, to be diffused to the surface, caused by travel motion；Such case, it can also be explained from another angle, i.e. continue not
Disconnectedly from inside PDMS body phases to those polymer molecules of its diffusion into the surface, migration, its result moved, be little by little by that
It has been involved in a bit by the large biological molecule of adsorption within the body phase of PDMS substrates, briefly, these adsorbed biologies
Macromolecular is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up the phenomenon of large biological molecule, its institute
Caused by influence, necessarily cause the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, the problem of PDMS substrates, is, its not only adsorption large biological molecule, and swallow up biological big point
Son, so, as the large biological molecule of experiment test object, its disappearance will not stop because surface saturation is adsorbed, and
It is, it is constantly adsorbed, also constantly swallowed up.
On PDMS substrates, its body phase is constantly swallowed up and tests showing for associated biomolecule macromolecular in related experiment test process
As, it is to say that another kind, which is explained, substantial amounts of Minute pores in PDMS body phases be present, associated biomolecule macromolecular by after adsorption,
Depression enters these Minute pores, and then is swallowed up；However, inventor thinks, those can allow the sky of miniature scale
Qi leel squeezes into the Minute pores therebetween, not equal to saying that they also can directly allow the large biological molecule of relative large scale to enter
Enter, both difference on yardstick are huge, must not make sweeping generalizations.Explanation is bypassed, in any case, as dependence test analysis object
Large biological molecule is adsorbed by PDMS substrate conduits inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective
Existing phenomenon.
, can be from containment PDMS surfaces pair in order to prevent swallow up effect of this PDMS substrate bodies relative to large biological molecule
The absorption of large biological molecule addresses, and method is chemically modified modification aiming at the PDMS material surface, for
For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described channel portion, by chemistry
The conduit inner surface of modification, can contain its absorption to large biological molecule, and then avoid large biological molecule quilt
PDMS substrate body phases are swallowed up；But or that old problem, that is, the chemical modification on PDMS material surface is modified it
Surface state afterwards can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface, migrate automatically
Process, can soon by that by surface chemical modification be modified conduit inner surface state become again script it is strong it is hydrophobic simultaneously
And the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS substrates its
Conduit inner surface is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, and can is enough
Reach and contain the absorption process of the PDMS substrate conduit inner surfaces to large biological molecule for a long time, and then prevent PDMS substrate bodies relative
The effect of swallowing up of large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life,
It is exactly a very intractable problem.The problem equally makes this area numerous specially as another problem addressed above
Industry personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.Should
Also be present many year in problem, so far, also not yet properly settled.
Third, the polydimethyl siloxane material, namely PDMS material, the active force very little wherein between polymer molecule,
The polymer molecule of low polymerization degree inside its body phase is in constantly into the dynamic process of surface migration, therefore, includes PDMS
After it has been formed, the surface topography in its inner-walls of duct face of chip can be because of described low polymerization degree polymer point for the chip of substrate material
Son moves about, pours into the surface and change, and the change of its pattern is somewhat similarly to Rheological Deformation, trickling deformation or creep and become
Pattern caused by shape institute is possible changes；In the higher PDMS material of some low polymerization degree component ratios, this kind somewhat similar
Can be showed in the phenomenon of Rheological Deformation, trickling deformation or the deformation of creep must be than more significant；This kind include PDMS substrates chip its
The dynamic of inner-walls of duct surface topography after shaping changes property, namely the described property or stream that are somewhat similarly to Rheological Deformation
Dropping down the property of deformation or the property of the deformation of creep, the word of rheology one is applied mechanically in this case, integration, also referred to as the rheological equationm of state or rheology become
Shape property, the dynamic of corresponding inner-walls of duct face pattern change, and this case is also referred to as rheology；As described above, because of this kind of material
Expect certain Rheological Deformation property as described above that its own has, its manufactured goods actually can at leisure Rheological Deformation and cause
The manufactured goods total form generation changes；Influence caused by its Rheological Deformation property, it is to be not easy to feel in terms of macroscopic perspective
Examine, still, for the very fine micro-fluidic chip of its internal structure, the rheological equationm of state can but cause bigger shadow
Ring, the fluid course namely the pipeline therefore, during pre-production formed, because the wall of its tube chamber of pipeline is main
It is the wall of PDMS materials, so, the cross-sectional area of the pipeline actually can gradually become because of rheology over time
It is small, stated differently, since the reason for the rheology, the natively finer pipeline that is flowed through for liquid sample
Its tube chamber the more can become the more narrow, in the case of flow time is sufficiently long, or even the pipeline tube chamber can partly close or all
Ground closes, and then make it that sample fluid course is thoroughly blocked, and closes with being even also not reaching to its luminal part of pipeline
The degree closed or fully closed, its fluid passage that can be provided of that pipeline narrow because of rheology, then, in pipeline
Its inner surface originally be exactly strongly it is hydrophobic in the case of, aqueous sample liquid is just more difficult to by becoming more so because of rheology
Narrow micro-channel, then, the influence of substrate rheology how is resisted, maintains the basic framework of the pipeline not occur seriously to become for a long time
The problem of shape, also, keep the long-term unobstructed of the pipeline is exactly a urgent need to resolve.
The content of the invention
The technical problem to be solved by the invention is to provide a package solution, solves totally above
A series of problems addressed, also, the solution is applied to hypotype swine flu diagnosis multichannel micro-fluidic chip field,
Form a kind of new hypotype swine flu diagnosis Multichannel device.
The present invention solves the technical problem by following scheme, and the device that the program provides is a kind of additional driving liquid
Stream is easy to the hypotype swine flu detection means of removal with component, and the structure of the device includes multichannel micro-fluidic chip, the miniflow
The structure of control chip includes being bonded to each other the substrate and cover plate of installing together, and the substrate and cover plate are plate object or sheet
The channel structure via mould pressing process or etching technics formation, the substrate are contained in thing, that face towards the cover plate of the substrate
Also contain and be connected and pierce being formed via mould pressing process, etching technics or simple drilling technology for the substrate with the channel structure
Window structure, be bonded to each other the substrate that is installed together and the cover plate and be built into jointly containing pipeline configuration and therewith
The micro-fluidic chip of connected liquid pool structure, the locations of structures of the pipeline are located at the interface area that the substrate is bonded to each other with the cover plate
Domain, its side of the window is blocked by the cover plate and opposite side opens, and the locations of structures of the window is exactly the structure bit of the liquid pool
Put, the quantity of the liquid pool is three, and its locations of structures of two liquid pools therein is located at the sample introduction end of the micro-fluidic chip, remaining
The liquid flowing in its chip when being located at the actual sample introduction test of the micro-fluidic chip of liquid pool its locations of structures terminal, the end
End is located remotely from each other with the sample introduction end, and one end of the pipeline is via the manifold shape turnout for being similarly positioned in the interface zone being bonded to each other
Respectively with the Liang Ge liquid pools UNICOM positioned at sample introduction end, the other end of the pipeline and the terminal positioned at the micro-fluidic chip
A remaining liquid pool UNICOM, and, the working electrode that is sequentially respectively installed in the pipeline on diverse location and to electricity
Pole and reference electrode, the working electrode is by conductive electrode and is attached on the conductive electrode and has embedded hypotype pig
The gold size sensitive membrane of influenza-specific antibody is formed, and the construction of the pipeline is in parallel construction, the pipeline in parallel construction by
Three laterals are in parallel to be formed, and the quantity of the working electrode is three, the installation position difference position of three working electrodes
In in three laterals, and, the specific antibody point in its top layer gold size sensitivity membrane structure of three working electrodes
It is not the three kinds of hypotype swine flu antibody materials that can be specifically bound to hypotype swine flu antigen, three kinds of antibody materials are respectively
Hypotype swine flu specific antibody H1N1、H3N2And H1N2, its material of the working electrode is that gold material or thermal decomposition are conductive high
Sheet or thread is presented in molecule material, its pattern of the working electrode, and emphasis is that its material of the substrate is dimethyl silicone polymer
Material, its surface of the substrate are the surfaces of primary form, the surface of the primary form its be intended to refer to and do not pass through any table
The surface of the primary form of the material of face chemical modification or any surface chemical modification, the structure of the device also include elastic force
Folder, two opposite clamping limb snap-in locations of the elastic clip are in the position of the neighbouring terminal of the micro-fluidic chip, at least
Fixation is attached described in one of which on clamping limb and is equiped with miniature ultrasonic transducer units, and, higher-order of oscillation electric signal passes
Transmission cable, one end and the miniature ultrasonic transducer units of the higher-order of oscillation electric signal transmission cable link together；The elastic clip
Provide a function of facilitating the device to disassemble；Its major function of the miniature ultrasonic transducer units is actual in micro-fluidic chip
When sample introduction is tested, the sample introduction end and the distance between the terminal and the miniature ultrasonic transducer units installation position difference are utilized
Difference on different and its ultrasonic intensity for being experienced, its interfacial tension of sample introduction end described in induced synthesis and the terminal its
Difference between interfacial tension, interfacial tension difference between the micro-fluidic chip both ends can the micro-fluidic chip this two
Pressure gap is formed between end, the pressure gap can drive sample solution to the end flow；It is soft and have elasticity this is poly-
Its function of the substrate of dimethyl siloxane material is included with its property to the strong absorption of ultrasonic wave, and ultrasonic wave is inhaled strongly
Receive, and thereby realize ultrasonic intensity within micro-fluidic chip terminal to the limited short distance between the sample introduction end
Rapid decrement；And many silica dioxide granules, many silica dioxide granules are filled in the pipeline, the pipe
Its tube chamber of road is filled up by many silica dioxide granules, and its particle size range of the silica dioxide granule is between 10 microns and 200
Between micron, the silica dioxide granule is its institute of silica crystalline particles, silica glass granule or silica composition
Account for inorganic glass particles of the percentage by weight more than 80%.
Its particle diameter of the silica dioxide granule can be that the basis between 10 microns and 200 microns is actually needed and any
The particle diameter specified, such as 10 microns, 30 microns, 70 microns, 100 microns, 150 microns or 200 microns of the particle diameter, etc..
The scope of further preferred its particle diameter of the silica dioxide granule is between 100 microns and 200 microns.
Its pattern of the silica dioxide granule is unlimited, and the silica dioxide granule for example can be spheric granules, rod-shaped particles, length
Cube shape particle or any amorphous granular.
The various silica dioxide granules manufacturing technology of itself is known technology.
Various forms, the silica dioxide granule market of all size are on sale.
The silica dioxide granule can also customize to specialized factory.
The preferred scope of its internal diameter of pipeline is between 500 microns and 1000 microns；It is but excellent compared to above-mentioned
The internal diameter of the pipeline scope of choosing, more tiny internal diameter of the pipeline or thicker internal diameter of the pipeline are also what this case was allowed.
Described miniature ultrasonic transducer units can certainly be all installed on two clamping limbs of the elastic clip；But
Only one miniature ultrasonic transducer units of installing are dealt with enough uses.
The gold size sensitive membrane is to be sufficiently mixed chitosan gold size solution and hypotype swine flu specific antibody solution
It is even, with point sample instrument point sample or specified structure position is coated on, and form its drying and forming-film.Hypotype in the gold size sensitive membrane
Swine flu specific antibody be horseradish peroxidase or glucose oxidase mark hypotype swine flu antibody, the gold
Glue sensitive membrane has been included as fixing above-mentioned each hypotype swine flu specific antibody and introducing complementary medium therein, the auxiliary
Property medium such as chitosan, cellulose acetate, gelatin be therein a kind of or their mixture.
The pipeline and the lateral and the manifold shape turnout in the microfluidic chip structure, in it
Footpath size may each be arbitrarily selected size, still, for as far as possible less with prepare liquid sample and reduction reagent loss etc.
Consideration, the pipeline and the lateral and the manifold shape turnout preferably select capillary level passage, institute
The passage for stating capillary level implies that its internal diameter passage suitable with the internal diameter of the capillary on ordinary meaning.The capillary is in it
The shape of cross section of portion's passage can be arbitrary shape, and the shape of cross section is for example circular, oval, square, rectangle, bar
Shape, naturally it is also possible to it is the linear of bending arbitrarily be present, also, the interior shape of the capillary is with the extension of pipeline,
The shape of cross section of different parts can also allow to be different shapes.Only for the word of capillary one, its art-recognized meanings is public
Know.
What is be related in structure is the electrode of microsize to electrode and reference electrode, and its electrode shape, which may each be, appoints
The selected shape of meaning, the arbitrarily selected shape such as square piece shape, rectangular patch, strip or round sheet etc..It is described
Art-recognized meanings to electrode and the reference electrode vocabulary of itself are known.
It is related to several liquid pools in this case microfluidic chip structure, the liquid pool is the pond shape or capsule for transitional liquid storage
Shape constructs, and its shape of the inner chamber of each liquid pool may each be arbitrarily selected shape, and the cavity shape is for example round
Cylindrical cavity shape, square column type cavity-like, oblong cavity shape or spherical hollow space shape etc..The size of the liquid pool can be any
Selected size, still, in order to as far as possible less with prepare liquid sample and reduce reagent loss, the liquid pool be preferably capable of with
The liquid pool of the microminiature of capillary matching.
Only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field,
It is known.
Various sizes, variously-shaped ultrasonic transducer are commercially available；Its size of commercially available miniature ultrasonic transducer units
It may diminish to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface its
It is known general technology for the professional in ultrasonic technology field for body.This case is not to this expansion superfluous words.
The word of elastic clip one art-recognized meanings of itself are known.
Only with regard to naked PDMS substrates itself conduit molding or lithographic technique for, be open-and-shut known technology；
Similarly, the technology of hole-opening is even more known simple technique on naked PDMS substrates.This case is not also to this expansion superfluous words.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the device can also include higher-order of oscillation electric signal generator；The higher-order of oscillation electric signal transmission cable
Its other end can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, come for the professional in ultrasonic technology field
Say, be simple and known；The higher-order of oscillation electric signal generator can customize to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 5 milliwatts and 5000 milliwatts
Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with
Between 12MHz.
This case device can further include some annexes certainly, and the annex is such as multiple tracks electrochemical workstation
Deng the art-recognized meanings of the multiple tracks electrochemical workstation are known.The each work being related in this case microfluidic chip structure
Electrode and to electrode and reference electrode etc., special it can get lines crossed and the multiple tracks electrochemical workstation via corresponding respectively
The corresponding interface coupled.It is described that special to get lines crossed be for by each phase of each electrode and the multiple tracks electrochemical workstation
Answer the private cable that interface is coupled to each other.The micro-fluidic chip in this case device, its structure can also include micro-valve,
The quantity of the micro-valve is unlimited, and according to being actually needed, the micro-valve can be installed in any need in the microfluidic chip structure
Position to be mounted；The word of micro-valve one is for the professional of micro fluidic chip technical field, the art-recognized meanings of itself
It is known；The micro-valve itself manufacturing technology and the use of technology is also known；The component that the micro-valve is not required.
The diameter of the working electrode can allow to be that any setting is easily installed the suitable diameter used, still,
It is recommending or say preferable its scope of the diameter between 0.1 micron to 2000 microns；The length of the working electrode can
To allow to be that any setting is easily installed the length used, it is however recommended to or to say the preferable length its scope be 1
Micron is between 15000 microns.
It is installed in by spraying or point sample instrument point sample or the coating of other appropriate process described in the working electrode surface layer
Gold size sensitive membrane, its thicknesses of layers can allow be any setting treat sample measuring liquid occur electrical signals response thickness,
It is however recommended to thickness preferable thickness is between 10 nanometers and 200 nanometers in other words.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, such as：Polypropylene,
Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, for example do
Into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and realized in the extremely short distance to ultrasonic wave
Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip
Using dimethyl silicone polymer it is used as the cover plate, and this case technical scheme is allowed.
Positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal should
Air line distance between a remaining liquid pool can be arbitrarily selected suitable distance, still, the distance as needed
Preferred value is between 3 centimetres and 7 centimetres.
Described its thickness of cover plate and substrate can allow be any setting the thickness for being easy to assembling, the thickness of recommendation or say
Preferable thickness is between 1.0 millimeters and 5.0 millimeters.Less thickness is advantageous to save material.
The word of elastic clip one art-recognized meanings of itself are known.
The elastic clip is commercially available.
The application method of this case micro-fluidic chip：
Itd is proposed first based on this case and the first public new liquid stream driving principle, its application of this case micro-fluidic chip are transported
Among work, the new liquid stream driving method is determined completely without involving any additional Micropump.
The interfacial tension difference that this case is formed between micro-fluidic chip both ends caused by the ultrasonic wave,
Driving liquid stream flows in the capillary channel of the triple channel micro-fluidic chip, right respectively using multi-channel electrochemical analyzer device
Three kinds of hypotype swine flu diagnostic antigens are detected.
The specific detection of this case micro-fluidic chip is as follows using step：
1st, blood serum sample liquid is added in micro-pipe road, under ultrasonic wave driving, various hypotype swine flu antigen molecules
The hypotype swine flu of the corresponding horseradish peroxidase-labeled embedded by gold size sensitive membrane on electrode surface in each passage is special
Property antibody capture.
2nd, the hypotype swine flu specific antibody of horseradish peroxidase-labeled resists with the hypotype swine flu in blood serum sample
Original shape is into immune complex.
3rd, using multi-channel electrochemical analyzer, the electron mediators such as catechol are added, it is above-mentioned using amperometric detection
Curent change caused by reaction, it is derived from the species and content of various analytes.
4th, result is subjected to comprehensive analysis, comprehensive diagnos is carried out to hypotype swine flu antigen.
It is an advantage of the invention that in elastic force described in the terminal of the micro-fluidic chip its close position snap-in location
Press from both sides, attached on the clamping limb of the elastic clip and miniature ultrasonic transducer units are installed, launch low work(with the miniature ultrasonic transducer units
The ultrasonic wave of rate, high-frequency band, meanwhile, using its strong absorbability to ultrasonic wave of dimethyl silicone polymer substrate, than
In shorter distance, it is, in from the terminal to the very short distance of only several centimeters yardsticks the sample introduction end, reach
Into the rapid decrement of ultrasonic intensity, the difference of the interfacial tension is thereby caused at the both ends of the micro-fluidic chip, is entered
And using the pressure gap between its both ends for being formed of the difference of the interfacial tension between the both ends, drive sample liquid stream
Flowed in the pipeline to the terminal direction.By this case liquid stream drive scheme, altogether dispense with traditional micro-
The equipment of pump etc；The micro-fluidic chip of Micropump is eliminated, its structure is more succinct, and its production process is less, and its cost of manufacture is more
Low, this kind of succinct structure is more beneficial for the low cost application for reaching micro-fluidic chip.
Its scheme of this case and by the pipeline except electrode installation take up space in addition to all clearance spaces whole
Filled up with the silica dioxide granule.Those are filled in the silica dioxide granule in pipeline, and this case is to its overall abbreviation titanium dioxide
Silicon grain filler, its function include support, withstand the tube chamber of the pipeline its inwall so that the tube chamber of the pipeline is from because of institute
State rheology and narrow, prevent its reduced cross-sectional area of the tube chamber of the pipeline or even close, so as to maintain the base of the pipeline for a long time
Serious deformation does not occur for this framework.
Numerous silica dioxide granule entities are mutually banked up under this case framework together at random in the pipeline, the titanium dioxide
The function of silicon grain filler, also include certainly with the hydrophilic surface of its silica dioxide granule, utilize each adjacent titanium dioxide
Mutual formed complications are continuous and its inner surface that is mutually close together by water-wetted surface of silicon grain has hydrophilic
The space of property, in the hydrophilic fluid channel of the pipeline Inner Constitution network morphology, although this complications can be continuous
All the time the fluid channel with hydrophilic nmature, then, inside the tube chamber of the pipeline simultaneously and a plurality of miniflow deposited
The effect of its synthesis of road, integrated, cumulative superposition in other words, is the equal of the relatively fine hydrophilic capillary of caliber
Passage；Its presence of the continuous hydrophilic fluid channel of the complications, the PDMS of the primary form is counteracted to a large extent
The flow resistance for liquid sample caused by the strong hydrophobic property in its surface of substrate；In other words, because of liquid sample, it is accounted for
The composition of maximum ratio is water, and liquid sample is substantially exactly the aqueous solution, therefore, the presence of the silica dioxide granule filler,
Can significantly overcome with the unmodified PDMS substrates of hydrophobic property and surface its to the aqueous solution it is incompatible, repel and
Barrier effect, so be greatly reduced belong to properties of Aqueous Solution sample liquid stream its be advanced through the resistance of pipeline.
Under this case framework, inside the tube chamber of the pipeline, the area of its lumen wall wall of former pipeline, because of described two
The rigid of silicon oxide particle filler ties up, the table of part hydrophobic property that still can also be exposed on lumen wall wall
Its area of face is already dramatically reduced, and the area on its still exposed part surface for belonging to hydrophobic property is much
Less than the wall surface area of the hydrophobic property of the lumen wall wall in the presence of no silica dioxide granule filler；
So, under this case framework, in its lumen of the pipeline, its remaining hydrophobic surface increases surface newly with hydrophilic part, and it is folded
Adding, comprehensive, summation, cumulative technique effect, is to form the surface for tending to be hydrophilic on the whole；In other words, in institute
State inside the tube chamber of pipeline, its interfacial tension between sample solution of the part surface of remaining hydrophobic property, plus parent
The part of aqueous nature increases its interfacial tension between sample solution of surface newly, that it is superimposed, comprehensive, summation, cumulative
Technique effect, be so that its solid-liquid interfacial tension inside the tube chamber of the pipeline synergy level off to capillary glass tube its
Interfacial tension between tube chamber inner surface and sample solution.
Therefore, exist in the pipeline under this case framework for having stated silica dioxide granule filler, while rely on this
Case sample liquid stream drive scheme, it becomes possible to the driving to sample liquid stream is reached with relatively low ultrasonic power, with this case mechanism
The test liquid is driven to flow to the end flow.
On the other hand, due to its rheological equationms of state of the PDMS as substrate material, it is filled in the pipeline
The interior silica dioxide granule filler, can gradually by constantly Rheological Deformation and to arest neighbors free space promote PDMS
The pipeline wall of material more tightly wraps up, and this process eventually causes those part two for pressing close to the lumen wall wall
Silicon oxide particle is stuck in original place or is stuck in original place or embedding in-situ silica dioxide granule and its embedded in original place, the part
Incarceration is together, described due to this reason each other for the remaining silica dioxide granule banked up with being mutually closely packed together
Silica dioxide granule filler just will not move in the pipeline easily, silica dioxide granule therein be also substantially all by
Original place is locked in, therefore the stream framework of the micro-fluidic chip can be kept for a long time.
The particle size range of its particle of this case silica dioxide granule filler between 10 microns and 200 microns, compared to
For general organic molecule, general large biological molecule, the particle diameter of its particle of this case silica dioxide granule filler can be rated as huge
Greatly, due to its huge particle diameter, and the inner surface of the surface of its polarity and the pipeline of strong hydrophobic PDMS materials can not
Mutually perhaps say and mutually melt, then, the huge silica dioxide granule of this kind of particle diameter can not be by the pipeline of PDMS materials
Surface is adsorbed, and can not more be swallowed up by the inner surface of the pipeline of PDMS materials, the huge titanium dioxide of this kind of particle diameter
Silicon grain surely not be fully sunk in easily or sink to completely PDMS materials its among intrinsic many Minute pores, and
Because above it is stated that the rheology the reason for, the silica dioxide granule filler can be stuck in original place in other words it is embedding
In original place without moving easily.
Inside the tube chamber of the pipeline, its lumen wall wall of former pipeline is entirely the surface of hydrophobic property originally, such as
Upper described, under this case framework, rigid because of the silica dioxide granule filler ties up, on lumen wall wall still also
Its area of the surface of part hydrophobic property that can be exposed is already dramatically reduced, and it is still exposed to belong to hydrophobicity
The area on the part surface of matter has been far smaller than the tube chamber in the presence of no silica dioxide granule filler
The wall surface area of the hydrophobic property of inwall wall；The reason for because of the rheology, it is close to those dioxies of lumen wall wall
Silicon carbide particle, little by little it is partially embedded into or is partly absorbed among the lumen wall wall, the dioxy among the state
Silicon carbide particle its towards the part surface of lumen wall wall with have occurred and that matching deformation the lumen wall wall it is tight
Closely connected conjunction, then, the matching is deformed and closely pasted with silica particles having occurred and that in lumen wall wall
The part wall of conjunction actually can not contact with flowing through the sample solution of pipeline, that is to say, that the wall of this part is
Through suction-operated can not be produced to the large biological molecule therein that flows through the sample solution of pipeline and organic molecule.Based on above-mentioned
Mechanism, then because fill up the pipeline the silica dioxide granule filler its presence, its lumen wall wall of the pipeline
The overwhelming majority in face is tightly covered, and these described walls tightly covered can not be with flowing through the pipeline
Sample solution contact, only remaining fraction be not brought into close contact by its surface of silica dioxide granule, tightly cover
Hydrophobic surface may be contacted with sample solution, in other words, under this case framework, large biological molecule in sample solution and have
Its direct touch opportunity with hydrophobic PDMS walls of machine molecule is significantly reduced, thus, the lumen wall faces of PDMS materials its
The adsorptive hindrance and interference of swallowing up of large biological molecule and organic molecule are greatly reduced；As described above, this case scheme
Be advantageous to exclude or weaken the adsorptive hindrance and swallow up interference, be advantageous to be lifted the reliability of correlation analysis test data.
As described above, this case silica dioxide granule filler, its function actually includes being similar to being directed to PDMS materials
Inner surface of pipeline carry out chemical modification effect, functioning as its this respect of this case is by the pipeline of the PDMS materials
Surface by the surface modification of hydrophobic property into the surface of hydrophilic nmature, still, with general, usual PDMS surface chemical modifications
Its hydrophilic chemical decorative layer retention time is short, hydrophilic effect can not keep situation prolonged enough different, particle described in this case
Huge silica dioxide granule filler, its particle being stuck by rheology can not both be moved easily, its huge particle diameter
Particle can not more be swallowed up easily by the inner-walls of duct of PDMS materials, and therefore, its from this respect is similar to surface chemical modification
Technique effect on see, its technique effect of this case, be form it is permanent, can not erase, can not consume, not be corroded, not by
Hydrophilic surface reforming layer swallowing up, being not dissolved, it is just comparable to one kind in its correlation table of PDMS base materials in effect
The permanent hydrophilic modifying superficial layer built on face.
It is related that the technical scheme of this case has dissolved its application to dimethyl silicone polymer substrate addressed above totally
A series of technical barriers.Based on this case scheme, the very cheap polydimethyl siloxane material of this kind is just possible to micro- at this
It is prepared by fluidic chip, production, using etc. field play bigger effect.
The miniature ultrasonic transducer units have been installed in fixation on the elastic clip its clamping limb in this case structure, and the structure carries
A function of facilitating the device to disassemble is supplied, in this way, the elastic clip just can together with miniature ultrasonic transducer units appended thereon
It is enough to be easily mutually disengaged with the micro-fluidic chip, then, the component that the part can freely depart from just can circulate benignly it is heavy
It is multiple to use many times；The architectural feature is advantageous to save the use cost of the device.
Brief description of the drawings
Fig. 1 is the schematic diagram of this case device embodiment chip part construction, it is shown that the vertical view of this structure
The structural form of chip internal pipeline and each electrode and each related liquid pool under angle, the elastic clip is not depicted in the figure
And the miniature ultrasonic transducer units set up thereon and other annexes.
Fig. 2 is its rough outside side view of this case device.
In figure, 1,2,8 be the different liquid pool of three installation positions respectively, and 3 be manifold shape turnout, and 4,9,12 be installing respectively
Position is different but three laterals parallel with one another for forming UNICOM's structure in parallel, and 5 be its top layer being installed in lateral 4
Specific antibody material in gold size sensitivity membrane structure is hypotype swine flu specific antibody H1N1Working electrode, 10 be installing
The specific antibody material in its top layer gold size sensitivity membrane structure in lateral 9 is hypotype swine flu specific antibody
H3N2Working electrode, 11 be the specific antibody material in its top layer gold size sensitivity membrane structure being installed in lateral 12
For hypotype swine flu specific antibody H1N2Working electrode, 6 be to electrode, and 7 be reference electrode, and 13 be elastic clip, 14,19 points
It is not two clamping limbs of elastic clip, 15 be the terminal of the micro-fluidic chip, and 16 be the sample introduction of the micro-fluidic chip
End, 17 be the substrate of dimethyl silicone polymer material, and 18 be cover plate, and 20 be miniature ultrasonic transducer units, and 21 be higher-order of oscillation electricity
Signal transmission cable, 22 be tension spring；The spring clamp structure in legend is only the legend structure of signal, actual elastic clip knot
Structure is not limited to the legend spring clamp structure；Arrow in legend indicate the micro-fluidic chip its in actual motion, by two
End pressure difference driving, the flow direction of its sample liquid stream.
Embodiment
In this case that Fig. 1 and Fig. 2 are shown embodiment, the structure of the device includes multichannel micro-fluidic chip, should
The structure of micro-fluidic chip includes being bonded to each other the substrate 17 and cover plate 18 of installing together, and the substrate 17 and cover plate 18 are
What is formed via mould pressing process or etching technics contained in plate object or tablet, that face towards the cover plate 18 of the substrate 17
Channel structure, the substrate 17 also containing be connected with the channel structure and pierce the substrate 17 via mould pressing process, etch work
The window structure that skill or simple drilling technology are formed, is bonded to each other the substrate 17 being installed together and is built jointly with the cover plate 18
Into the micro-fluidic chip containing pipeline configuration and the liquid pool structure being attached thereto, the locations of structures of the pipeline is located at the substrate
17 interface zones being bonded to each other with the cover plate 18, its side of the window is blocked by the cover plate 18 and opposite side opens, the window
Locations of structures be exactly the liquid pool locations of structures, the quantity of the liquid pool is three, three liquid pools be respectively liquid pool 1,
Liquid pool 2, liquid pool 8, two liquid pools therein are the sample introduction end 16 that liquid pool 1 and liquid pool 2 its locations of structures are located at the micro-fluidic chip,
A remaining liquid pool is liquid flowing in its chip when liquid pool 8 its locations of structures is located at the micro-fluidic chip actual sample introduction test
Terminal 15, the terminal 15 is located remotely from each other with the sample introduction end 16, and one end of the pipeline is via being similarly positioned in the boundary being bonded to each other
The manifold shape turnout 3 in face region is liquid pool 1 and the UNICOM of liquid pool 2 with two liquid pools positioned at sample introduction end 16 respectively, the pipeline
The other end and a remaining liquid pool for the terminal 15 positioned at the micro-fluidic chip are the UNICOM of liquid pool 8, and, sequentially divide
The working electrode that is not installed in the pipeline on diverse location and to electrode and reference electrode, the working electrode is by conduction
Property electrode and be attached on the conductive electrode embedded hypotype swine flu specific antibody gold size sensitive membrane form, should
The construction of pipeline is in parallel construction, and the pipeline in parallel construction is made up of three lateral parallel connections, the working electrode
Quantity be three, the installation positions of three working electrodes respectively in three laterals, and, three works
The specific antibody made in electrode its top layer gold size sensitivity membrane structure can be specifically bound to hypotype swine flu antigen respectively
Three kinds of hypotype swine flu antibody materials, three kinds of antibody materials are hypotype swine flu specific antibody H respectively1N1、H3N2And H1N2,
For specific expansion, 5 be that specific antibody material in its top layer gold size sensitivity membrane structure being installed in lateral 4 is Asia
Type swine flu specific antibody H1N1Working electrode, 10 be in its top layer gold size sensitivity membrane structure being installed in lateral 9
Specific antibody material be hypotype swine flu specific antibody H3N2Working electrode, 11 are installed in lateral 12
Specific antibody material in its top layer gold size sensitivity membrane structure is hypotype swine flu specific antibody H1N2Working electrode, institute
It is gold material or thermal decomposition conducting polymer material to state working electrode its material, its pattern of the working electrode present sheet or
Thread, emphasis is, its material of the substrate 17 is dimethyl silicone polymer material, and its surface of substrate 17 is the table of primary form
Face, the surface of the primary form its be intended to refer to not by any surface chemical modification or any surface chemical modification should
The surface of the primary form of material, the structure of the device also include elastic clip 13, two opposite clamping limbs of the elastic clip 13
14th, 19 snap-in locations are in the position of the neighbouring terminal 15 of the micro-fluidic chip, a clamping limb at least in
Attach to fix i.e. on clamping limb 14 or clamping limb 19 and be equiped with miniature ultrasonic transducer units 20, in this example, the miniature ultrasonic changes
Energy device 20 is to attach to be installed on clamping limb 19, and, higher-order of oscillation electric signal transmission cable 21, the higher-order of oscillation telecommunication
One end of number transmission cable 21 links together with the miniature ultrasonic transducer units 20；The elastic clip 13 provides a convenience should
The function that device is disassembled；Its major function of the miniature ultrasonic transducer units 20 is the profit in micro-fluidic chip actual sample introduction test
With the sample introduction end 16 and the distance between the terminal 15 and the installation position of miniature ultrasonic transducer units 20 difference and
Difference on its ultrasonic intensity experienced, its interfacial tension of sample introduction end 16 described in induced synthesis and described its boundary of terminal 15
Difference between the tension force of face, the interfacial tension difference between the micro-fluidic chip both ends can be at the both ends of the micro-fluidic chip
Between form pressure gap, the pressure gap can drive sample solution to be flowed to the terminal 15；It is soft and have elasticity this is poly-
Its function of the substrate 17 of dimethyl siloxane material is included with its property to the strong absorption of ultrasonic wave, ultrasonic wave is carried out strong
Absorb, and thereby realize ultrasonic wave within micro-fluidic chip terminal 15 to the limited short distance between the sample introduction end 16
The rapid decrement of intensity；And many silica dioxide granules, many silica dioxide granules are filled in the pipeline,
Its tube chamber of the pipeline is filled up by many silica dioxide granules, and its particle size range of the silica dioxide granule is between 10 microns
Between 200 microns, the silica dioxide granule is silica crystalline particles, silica glass granule or silica composition
Its inorganic glass particles of shared percentage by weight more than 80%.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its try
The flow direction of sample liquid stream.
Miniature ultrasonic transducer units 20 and high frequency vibrating that the elastic clip 13 is depicted without in Fig. 1 and is set up thereon
Swing electric signal transmission cable 21；Also, the higher-order of oscillation electric signal generator and multiple tracks electricity are depicted without in Fig. 1 and Fig. 2
The associate members such as chem workstation.
Involved miniature ultrasonic transducer units 20 are commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal transmission cable 21 is commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also determine to relevant manufacturers
System.
Each working electrode in this example structure and can be respectively via each special electricity to electrode and reference electrode
Cable or say is being got lines crossed respectively with the corresponding cable interface of the multiple tracks electrochemical workstation as annex or saying interface connection of getting lines crossed.
Involved elastic clip 13 is commercially available；The structural form of elastic clip 13 in legend is only an example, this case elastic force
The structural form of folder is not limited to the structure type in the legend；Can meet that this case organization plan uses is commercially available alternative
Spring clamp structure and moulding and size etc., quality is various, and specific elastic clip pattern can be selected as needed.
Claims (10)
1. additional driving liquid stream is easy to the hypotype swine flu detection means of removal with component, the structure of the device includes multichannel
Micro-fluidic chip, the structure of the micro-fluidic chip include being bonded to each other the substrate and cover plate of installing together, the substrate and lid
Piece is plate object or tablet, and that face towards the cover plate of the substrate is contained to be formed via mould pressing process or etching technics
Channel structure, the substrate also containing be connected with the channel structure and pierce the substrate via mould pressing process, etching technics
Or the window structure that simple drilling technology is formed, it is bonded to each other the substrate being installed together and has been built into jointly with the cover plate and contains
The micro-fluidic chip of liquid pool structure for having pipeline configuration and being attached thereto, the locations of structures of the pipeline are located at the substrate and the lid
The interface zone that piece is bonded to each other, its side of the window is blocked by the cover plate and opposite side opens, and the locations of structures of the window is just
It is the locations of structures of the liquid pool, the quantity of the liquid pool is three, and its locations of structures of two liquid pools therein is located at the miniflow
The sample introduction end of chip is controlled, its locations of structures of a remaining liquid pool is located at when the actual sample introduction of the micro-fluidic chip is tested in its chip
The terminal of liquid flowing, the terminal is located remotely from each other with the sample introduction end, and one end of the pipeline is via being similarly positioned in what this was bonded to each other
The manifold shape turnout of interface zone is micro- with being located at this with the Liang Ge liquid pools UNICOM positioned at sample introduction end, the other end of the pipeline respectively
A remaining liquid pool UNICOM for the terminal of fluidic chip, and, sequentially it is respectively installed in diverse location in the pipeline
On working electrode and to electrode and reference electrode, the working electrode is by conductive electrode and is attached to the electric conductivity
The gold size sensitive membrane for having embedded hypotype swine flu specific antibody on electrode is formed, and the construction of the pipeline is in parallel construction, institute
State and be made up of in the pipeline of parallel construction three lateral parallel connections, the quantity of the working electrode is three, this three work
The installation position of electrode is located in three laterals respectively, and, its top layer gold size sensitive membrane of three working electrodes
Specific antibody in structure is the three kinds of hypotype swine flu antibody materials that can be specifically bound to hypotype swine flu antigen respectively,
Three kinds of antibody materials are hypotype swine flu specific antibody H respectively1N1、H3N2And H1N2, its material of the working electrode is yellow
Sheet or thread is presented in golden material or thermal decomposition conducting polymer material, its pattern of the working electrode, it is characterised in that the base
Its material of piece is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the surface of the primary form its
The surface of the primary form for the material for not passing through any surface chemical modification or any surface chemical modification is intended to refer to,
The structure of the device also includes elastic clip, the neighbour of two opposite clamping limb snap-in locations of the elastic clip in the micro-fluidic chip
The position of the nearly terminal, fixation is attached on a clamping limb at least in and is equiped with miniature ultrasonic transducer units,
And higher-order of oscillation electric signal transmission cable, one end of the higher-order of oscillation electric signal transmission cable and the miniature ultrasonic transducing
Device links together；The elastic clip provides a function of facilitating the device to disassemble；Its is main for the miniature ultrasonic transducer units
Function is in the actual sample introduction test of micro-fluidic chip, utilizes the sample introduction end and the terminal and the miniature ultrasonic transducing
Difference in the distance between device installation position difference and its ultrasonic intensity experienced, sample introduction end described in induced synthesis
Difference between its interfacial tension and the terminal its interfacial tension, the interfacial tension difference between the micro-fluidic chip both ends
Pressure gap can be formed between the both ends of the micro-fluidic chip, the pressure gap can drive sample solution to the terminal stream
It is dynamic；Softness simultaneously has its function of the substrate of the dimethyl silicone polymer material of elasticity including with its property to the strong absorption of ultrasonic wave
Matter, ultrasonic wave is absorbed strongly, and thereby in micro-fluidic chip terminal to the limited short distance between the sample introduction end
The rapid decrement of ultrasonic intensity is realized within；And many silica dioxide granules, many silica dioxide granules are filled out
Fill in the pipeline, its tube chamber of the pipeline is filled up by many silica dioxide granules, its grain of the silica dioxide granule
For footpath scope between 10 microns and 200 microns, the silica dioxide granule is silica crystalline particles, silica glass
Grain or silica composition its inorganic glass particles of shared percentage by weight more than 80%.
2. additional driving liquid stream according to claim 1 is easy to the hypotype swine flu detection means of removal with component, its
It is characterised by, the pipeline and the lateral and the manifold shape turnout are capillary channel.
3. additional driving liquid stream according to claim 1 is easy to the hypotype swine flu detection means of removal with component, its
It is characterised by, the thermal decomposition conducting polymer is the electric conductivity formed by polyimides or polyacrylonitrile after anoxybiotic is heat-treated
Material.
4. additional driving liquid stream according to claim 1 is easy to the hypotype swine flu detection means of removal with component, its
Be characterised by, the width or diameter of the working electrode between 0.1 micron to 2000 microns, and, the working electrode
Length between 1 micron to 15000 microns.
5. additional driving liquid stream according to claim 1 is easy to the hypotype swine flu detection means of removal with component, its
It is characterised by, the thickness of the gold size sensitive membrane is between 10 nanometers and 200 nanometers.
6. additional driving liquid stream according to claim 1 is easy to the hypotype swine flu detection means of removal with component, its
It is characterised by, the cover plate its material in structure is dimethyl silicone polymer material.
7. additional driving liquid stream according to claim 1 is easy to the hypotype swine flu detection means of removal with component, its
Be characterised by, positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal should
Air line distance between a remaining liquid pool is between 3 centimetres and 7 centimetres；Described its thickness of cover plate and substrate in structure
Between 1.0 millimeters and 5.0 millimeters.
8. additional driving liquid stream according to claim 1 is easy to the hypotype swine flu detection means of removal with component, its
It is characterised by, its particle size range of the silica dioxide granule is between 100 microns and 200 microns, its inside diameter ranges of the pipeline
Between 500 microns and 1000 microns.
9. additional driving liquid stream according to claim 1 is easy to the hypotype swine flu detection means of removal with component, its
Be characterised by, the structure of the device also includes higher-order of oscillation electric signal generator, the higher-order of oscillation electric signal transmission cable its
The other end is connected with the higher-order of oscillation electric signal generator.
10. additional driving liquid stream according to claim 1 is easy to the hypotype swine flu detection means of removal with component, its
It is characterised by, for its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units between 5 milliwatts and 5000 milliwatts, this is miniature
The frequency of its ultrasonic wave operationally launched of ultrasonic transducer is between 100KHz and 12MHz.
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| CN201610329080.4A CN107349978A (en) | 2016-05-10 | 2016-05-10 | Additional driving liquid stream is easy to the hypotype swine flu detection means of removal with component |
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| CN201610329080.4A CN107349978A (en) | 2016-05-10 | 2016-05-10 | Additional driving liquid stream is easy to the hypotype swine flu detection means of removal with component |
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Cited By (1)
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| CN114247493A (en) * | 2020-10-20 | 2022-03-29 | 深圳铭毅基因科技有限公司 | Fixing device for chip for chemical reaction |
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| CN107349980A (en) | The hypotype swine flu detection device that fluid driving can be removed rapidly with component | |
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| CN107754916A (en) | The multichannel micro-fluidic chip device of a variety of hypotype swine flus is detected simultaneously | |
| CN107754902A (en) | Its substrate of chip uses the cholera diagnosis micro fluidic device of strong hydrophobic material | |
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Application publication date: 20171117 |