CN107081179A - The micro flow control chip device of substrate material is used as using dimethyl silicone polymer - Google Patents

The micro flow control chip device of substrate material is used as using dimethyl silicone polymer Download PDF

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Publication number
CN107081179A
CN107081179A CN201610100299.7A CN201610100299A CN107081179A CN 107081179 A CN107081179 A CN 107081179A CN 201610100299 A CN201610100299 A CN 201610100299A CN 107081179 A CN107081179 A CN 107081179A
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micro
fluidic chip
substrate
ultrasonic wave
silicone polymer
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李榕生
葛宇杰
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/12Specific details about materials
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/16Surface properties and coatings
    • B01L2300/161Control and use of surface tension forces, e.g. hydrophobic, hydrophilic

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micromachines (AREA)

Abstract

The present invention relates to a kind of micro flow control chip device for taking new fluid type of drive, belong to analysis testing field.The substrate of micro-fluidic chip is made using dimethyl silicone polymer i.e. PDMS that is cheap and easily processing, there are some problems;Its surface of the PDMS material is strongly hydrophobic, and targetedly surface is modified or surface modification, and its effect is difficult to persistently, and this case aims to solve the problem that the serial relevant issues.This case main points are, the selected PDMS with ecosystem surface of substrate, and fastening is positioned at the test liquid stream terminal of the micro-fluidic chip its neighbor positions manually by the hinge-type fixture for being attached to miniature ultrasonic transducer units, simultaneously, ultrasonic wave damper is installed at the sample introduction end of the micro-fluidic chip, reach ultrasonic intensity rapid decrement in short distance, and thus form interfacial tension difference at the two ends of the chip, thereby drive test liquid stream to be flowed along originally hydrophobic capillary channel to the terminal direction.

Description

The micro flow control chip device of substrate material is used as using dimethyl silicone polymer
Technical field
The present invention relates to a kind of micro flow control chip device using dimethyl silicone polymer as substrate material, belong to analysis test neck Domain.
Background technology
For the microflow control technique overall general picture of itself, it may refer to famous micro-fluidic expert Mr. Lin Ping Cheng and go out not long ago Monograph " diagram Microfluid based Lab on a chip ", the monograph is published via Science Press, and the monograph is for microflow control technique Past, now, and, in terms of vision of the future etc., suffer from detail, be deep into long discussion of detail.
The field that micro-fluidic chip is often applied to includes carrying out various analyses, detection to the sample solution containing large biological molecule.
The basic framework of micro-fluidic chip, including it is etched with the substrate of small fluid course and the cover plate fit together therewith, Small fluid course on the substrate, assembling upper cover plate before, it is apparent on see to be exactly some micro-channels, wait until thereon After covering cover plate, just real closure forms the small fluid course, and the conduit inner surface of the micro-channel is together with surround this The part cover plate of micro-channel constitutes described small fluid course together;So, it is clear that this after assembling is completed is small Fluid course, the major part of its inner surface area is the inner surface area of that micro-channel, in other words, in the micro-channel The state or property on surface substantially determine the integrality or property of the small fluid course;Therefore say, this is built in base The inner surface state or inner surface property of micro-channel on piece are key factors;In principle, it is any to keep or protecting substantially The material of its solid forms is held, can be used to make substrate and cover plate, such as, the material that can act as substrate and cover plate can be with It is monocrystalline silicon piece, quartz plate, sheet glass, high polymer such as dimethyl silicone polymer, polymethyl methacrylate, makrolon etc. Deng;Certainly, the selection of substrate and the selection of cover plate be able to can also be differed with identical;From material consumption, manufacture difficulty and From the point of view of in terms of application popularization prospect etc., between these materials exist not small difference, the especially selection of that substrate, influence compared with Greatly.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, so Substrate on make that micro-channel is extremely simple, and the lower cost for material makes substrate with the polydimethyl siloxane material, Micro-channel is being suppressed or etched thereon, and is being engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes, It is a kind of more satisfactory selection seemingly;Certainly, patch material can also select to use cheap polydimethyl siloxane material: So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, makes extremely simple, seems and should also be as It is extremely easy to popularize, promotes.
But, thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that the letter PDMS that abridges is referred to, itself are a kind of strong Hydrophobic material, micro-channel is built on this material, if without being operated for the modified of the micro-channel surface, then, After overall assembling is completed, that is, cover after cover plate, because the micro-channel its inner surface in structure occupies most liquid circulation The inner surface in road, then, its strong hydrophobic property of the PDMS micro-channels inner surface, is deciding factor, it can cause class Be similar to the aqueous solution the fine liquid stream of polar liquid by becoming very difficult, its flow resistance is big, or even general Micropump is all It is difficult to promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar; Therefore, among prior art, modification is modified particular for the micro-channel inner surface on the PDMS material, is necessary Operation;So, this is pretty troublesome for the modified operation of PDMS micro-channel inner surfacesThat falls nor this problem, structure Perplex into serious technical, be another problem:PDMS polymer molecules tool inside its body phase of this PDMS material substrate There are PDMS polymer molecules inside the characteristic for diffusing to the surface, migrating automatically, this substrate body phase to diffuse to the surface, move automatically The characteristic of shifting, will cause the state after modification by its inner surface of that micro-channel of surface modifying and decorating can not maintain foot Enough long time, the holding time for micro-channel its inner surface state after that is surface-modified is substantially only sufficient to complete in laboratory The time of portion's test experiments needs;In other words, by surface modification or the PDMS micro-channel inner surfaces of surface modification, its The surface state that is formed can not be lasting after modification or after saying modification, but soon automatically tend to or say and become surface again and change Surface state before property, returns to the strong hydrophobic surface state of that script in the shorter time, then, just think, Such micro-fluidic chip can largely make, mass storage, be widely popularized, and answer is it is obvious that is, impossible. Micro-channel on this PDMS material, can not pump similar to the fine liquid stream of polar solvent of the aqueous solution if not doing surface modification Send and pass through, chip also cannot just be used;And if having done surface modification, the state after its modification can not be persistently kept again, also Being equally can not popularization and application.
So, how to accomplish that substrate can either be made using cheap PDMS material, and table in the micro-channel can be released Face decorating state can not persistently, chip can not largely make, largely lay in so that be widely popularized it is such a make this area it is numerous specially The puzzlement that industry personnel are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, above it is stated that, its surface is strongly hydrophobic, this strong hydrophobic Material surface and also another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and And, these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into gradually deep, directly It is trapped in heavy within the body phase of PDMS substrates, in fact, this process is partly also due to inside PDMS material body phase Polymer molecule, which has, to be diffused to the surface, caused by travel motion;Such case, can also be explained from another angle, i.e. Continuously from inside PDMS body phases to its diffusion into the surface, migration those polymer molecules, its result moved, be by Gradually those are involved within the body phase of PDMS substrates by the large biological molecule of adsorption, briefly, these are inhaled Attached large biological molecule is exactly to be swallowed up by PDMS substrate body phases;So, this PDMS substrates body phase swallows up large biological molecule Phenomenon, the influence caused by it necessarily causes the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, be the problem of PDMS substrates, its not only adsorption large biological molecule, and swallow up large biological molecule, So, as the large biological molecule of experiment test object, its disappearance will not stop because the absorption of surface saturation, but, no It is disconnected adsorbed, also constantly swallowed up.
On PDMS substrates in related experiment test process its body phase constantly swallow up test associated biomolecule macromolecular phenomenon, separately It is to say that one kind, which is explained, there are substantial amounts of Minute pores in PDMS body phases, and associated biomolecule macromolecular is by after adsorption, depression Into these Minute pores, and then swallowed up;However, inventor thinks, those can allow the air point of miniature scale Son squeezes into the Minute pores therebetween, and being not equal to them also can directly allow that the large biological molecule of relative large scale enters, and two Person's difference on yardstick is huge, must not make sweeping generalizations.Explanation is bypassed, in any case, the biology of object is analyzed as dependence test Macromolecular is adsorbed by PDMS substrate micro-channels inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective The phenomenon of presence.
, can be from containment PDMS surfaces to life in order to prevent this PDMS substrate bodies relative to the effect of swallowing up of large biological molecule The absorption of thing macromolecular is addressed, and method is chemically modified modification aiming at the PDMS material surface, for For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described micro-channel part, by changing The micro-channel inner surface of modification is learned, its absorption to large biological molecule can be contained, and then avoid large biological molecule quilt PDMS substrate body phases are swallowed up;But, or that old problem, that is, the chemical modification on PDMS material surface changes Surface state after property can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface automatically, The process of migration, soon can become that micro-channel inner surface state being modified by surface chemical modification again script strong and dredge Water and the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS Its micro-channel inner surface of substrate is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, can reach again Growth stage contains absorption process of the PDMS substrate micro-channel inner surfaces to large biological molecule, and then prevents PDMS substrate body phases The effect of swallowing up to large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life, It is exactly a very intractable problem.The problem makes the numerous specialties in this area as another problem addressed above, equally Personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.The hardly possible Also be present many year in topic, so far, also not yet properly settled.
The content of the invention
The technical problems to be solved by the invention are to provide a package solution, while solving two addressed above Problem.
The present invention solves the technical problem by following scheme, and the device that the program is provided is one kind with dimethyl silicone polymer As the micro flow control chip device of substrate material, the structure of the device includes micro-fluidic chip, and the structure of the micro-fluidic chip includes The substrate and cover plate of installing bonded to each other together, its test liquid of the micro-fluidic chip flow into the end at sample end and the sample fluid flow End is located remotely from each other, and the distance between the sample introduction end and the terminal are between 3 centimetres and 10 centimetres, and emphasis is, the substrate its Material is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the surface of the primary form its be meant to Be the material not no by any surface chemical modification or any surface chemical modification primary form surface, the device Structure also includes hinge-type fixture, hinge-type fixture its appearance profile likeness in form hinge, and the hinge-type fixture is by being hinged one Rise two hinges and matched through a fastening screw of two hinges and one with the fastening screw and with the fastening The nut for being used for fastening and hand break loose manually that screw connects together is constituted, and two hinges of the hinge-type fixture are each with its end The tip is mutually drawn close and clamps the micro-fluidic chip, and the tip is positioned at the locations of structures of the neighbouring terminal of the micro-fluidic chip On, fixation is attached in a hinge at least in and is equiped with miniature ultrasonic transducer units, and, higher-order of oscillation telecommunication Number transmission cable, one end and the miniature ultrasonic transducer units of the higher-order of oscillation electric signal transmission cable link together;The hinge Formula fixture facilitates the function that the device is disassembled there is provided one;Its major function of the miniature ultrasonic transducer units is in micro-fluidic chip During the test of actual sample introduction, the ultrasonic wave launched using it reduces the inwall of sample solution and its inner passage of micro-fluidic chip Between interfacial tension, can be compatible, also, utilize the sample introduction end and the terminal and the miniature ultrasonic transducing Difference in the distance between device installation position difference and its ultrasonic intensity experienced, sample introduction end described in induced synthesis its Interfacial tension difference between difference between interfacial tension and the terminal its interfacial tension, the micro-fluidic chip two ends can be Pressure gap is formed between the two ends of the micro-fluidic chip, the pressure gap can drive sample solution to the end flow;Should Miniature ultrasonic transducer units its functions also include with its ultrasonic wave launched check large biological molecule contained in sample its Absorption on its inner passage inner surface of the micro-fluidic chip, and then check the substrate of the dimethyl silicone polymer material its body phase pair The effect of swallowing up of the large biological molecule;Softness simultaneously has its function of the substrate of the dimethyl silicone polymer material of elasticity including right with its The property that ultrasonic wave absorbs strongly, is absorbed strongly to ultrasonic wave, and thereby in micro-fluidic chip terminal to the sample introduction end Between limited short distance within realize the rapid decrement of ultrasonic intensity;And, ultrasonic wave damper, ultrasonic wave abatement Its profile of device is in block, tabular or bar-shaped;Its material of the ultrasonic wave damper is microcellular rubber, sponge rubber or microporous polyurethane Elastomer;And, elastic clip, the elastic force accompanies two clamping limbs, and the medial surface of each clamping limb is interior with another clamping limb Side is relative, and the medial surface of one of clamping limb is close to the sample introduction end of the micro-fluidic chip, the medial surface of another clamping limb It is close to the ultrasonic wave damper, the elastic clip is with its gripping strength by the sample introduction end of the micro-fluidic chip and the ultrasonic wave damper Clip together bonded to each otherly, the strong consumption to ultrasonic wave that the ultrasonic wave damper is brought with its material, further The fast express delivery of ultrasonic intensity is realized in support within micro-fluidic chip terminal to the limited short distance between the sample introduction end Subtract, and thereby further expand the difference between described its interfacial tension of sample introduction end and the terminal its interfacial tension.
Described miniature ultrasonic transducer units can certainly be all installed in two hinges;But, only installing one is micro- Type ultrasonic transducer is dealt with enough to be used.
The word of hinge one art-recognized meanings of itself are known.
It is public only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field Know.
Various sizes, variously-shaped ultrasonic transducer are commercially available;Commercially available miniature ultrasonic transducer units its sizes can be with It is small to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface itself and Speech, is known general technology for the professional in ultrasonic technology field.This case is not to this expansion superfluous words.
Only with regard to naked PDMS substrates itself micro-channel molding or lithographic technique for, be open-and-shut known technology;Together Sample, the technology of hole-opening is even more known simple technique on naked PDMS substrates.This case is not also to this expansion superfluous words.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the device can also include higher-order of oscillation electric signal generator;Its is another for the higher-order of oscillation electric signal transmission cable One end can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, for the professional in ultrasonic technology field, be It is simple and known;The higher-order of oscillation electric signal generator can be customized to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units be the milliwatt of Jie's hand 2 with 9000 milliwatts it Between;The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with Between 12MHz.
This case device can further include some annexes certainly, and the annex can for example be used cooperatively with micro-fluidic chip Electrically or optically detection device.The equipment that the electricity, optics etc. are used cooperatively with micro-fluidic chip, may refer to described Monograph.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, for example:Polypropylene, glass Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, such as do Realized into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and in the extremely short distance to ultrasonic wave Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip It is used as the cover plate using dimethyl silicone polymer, is also that this case technical scheme is allowed.
Described its thickness of cover plate and substrate can allow be any setting be easy to assembling thickness, the thickness of recommendation or say preferably Thickness be between 1.0 millimeters and 5.0 millimeters.Less thickness is conducive to saving material.
Described its size of ultrasonic wave damper is not limited, and described its length of ultrasonic wave damper, width, thickness may each be basis Need the arbitrary size of setting.
It is known in field of rubber technology only for the word of microcellular rubber one art-recognized meanings of itself.
The microcellular rubber is commercially available.
It is known in field of rubber technology only for the word of sponge rubber one art-recognized meanings of itself.
The sponge rubber is commercially available.
It is known in technical field of macromolecules only for the word of microporous polyurethane elastomer one art-recognized meanings of itself.
The polyurethane micropore elastomer material is commercially available.
The microcellular rubber preferred microporous silicon rubber or micropore fluorubber.
It is known in field of rubber technology only for the word of micropore silicon rubber one art-recognized meanings of itself.
The microporous silicon Rubber market is on sale.
It is known in field of rubber technology only for the word of micropore fluorubber one art-recognized meanings of itself.
The micropore fluorubber is commercially available.
The word of elastic clip one art-recognized meanings of itself are known.
The elastic clip is commercially available.
It is an advantage of the invention that its close position positions the hinge-type fixture in the terminal of the micro-fluidic chip, with The miniature ultrasonic transducer units installed, the low-power launched using it, high-frequency band are attached in its hinge of the hinge-type fixture Ultrasonic wave so that without surface chemical modification or surface chemical modification strong hydrophobic micro-fluidic chip internal pipeline its Compatibility between tube wall and the test object aqueous solution is significantly increased, and this passes through possible there is provided a reality for test liquid stream Property;Meanwhile, using its strong absorbability to ultrasonic wave of dimethyl silicone polymer substrate, in shorter distance, also Be, from the terminal to the very short distance of only several centimeters yardsticks the sample introduction end in, reach the quick of ultrasonic intensity Successively decrease, the difference of the interfacial tension is thereby caused at the two ends of the micro-fluidic chip, and then, using between the two ends Pressure gap between its two ends for being formed of the difference of interfacial tension, driving test liquid stream is strongly hydrophobic in such a script Capillary channel in the terminal direction flow.By this case liquid stream drive scheme, entirely without must be to the polydimethylsiloxanes The substrate and its internal pipeline of alkane material carry out any surface chemical modification or chemical modification, have altogether dispensed with the surface chemistry and have repaiied The laborious procedures of decorations or chemical modification;And altogether dispense with the equipment of traditional Micropump etc;On the other hand, this is low The ultrasonic wave of power, high-frequency band, additionally it is possible to contain the large biological molecule in sample in the literalness naked polydimethylsiloxanes Absorption on its inner surface of pipeline of alkyl piece, and then contain its body phase of the dimethyl silicone polymer substrate to the large biological molecule The effect of swallowing up;Because described suction-operated and the described effect of swallowing up effectively are contained, therefore, dependence test result will It is better able to objectively reflect actual conditions.
Because the surface chemical modification or surface chemistry that need not carry out for the dimethyl silicone polymer substrate its relevant surfaces change Property operation, therefore, this surface chemical modification layer or surface chemical modification layer not need exist, then, the poly- diformazan Its body phase interior polymer molecule of radical siloxane substrate constantly diffuses to the surface automatically, migrate caused by it to the surface chemistry The damaging influence of decorative layer or surface chemical modification layer is also just not present.
This case its installation position is located at the ultrasonic wave damper at the sample introduction end, from its terminal of this case micro-fluidic chip to Said on the sport technique segment that ultrasonic intensity rapid decrement is realized in the such short distance in sample introduction end, its effect of the ultrasonic wave damper is suitable In the size expansion device of micro-fluidic chip, in other words, the ultrasonic wave damper its function as and significantly extend miniflow Its terminal of chip is controlled the distance between to sample introduction end, is the equal of from several centimetres of extension of script to more than ten centimetres of length Or twenties centimetres of length or longer length, so so that described terminal to the ultrasonic wave between sample introduction end in short distance The sport technique segment of intensity rapid decrement can relatively easily be reached, also, the size expansion device namely the ultrasonic wave damper Its installation and removal is all very convenient, it is important that it can also be reused.
The technical scheme of this case has dissolved its application related one to dimethyl silicone polymer substrate addressed above totally Row technical barrier.Based on this case scheme, this kind very cheap polydimethyl siloxane material is just possible in the micro-fluidic chip Prepare, production, using etc. field play bigger effect.
The miniature ultrasonic transducer units have been installed in fixation in the hinge-type fixture its hinge in this case structure, and the structure is provided One facilitates the function that the device is disassembled, in this way, the hinge-type fixture is together with appended miniature ultrasonic transducer units in its hinge Just easily it can be mutually disengaged with the micro-fluidic chip, then, the component that the part can freely depart from just can circulate benignly ground Reuse many times;The architectural feature is conducive to saving the use cost of the device.
Brief description of the drawings
Fig. 1 is its rough outside side view of this case micro flow control chip device.
In figure, 1 is hinge-type fixture, and 2 indicate linkwork positions, and 3 be fastening screw, and 4 be higher-order of oscillation telecommunication Number transmission cable, 5 be miniature ultrasonic transducer units, and 6,11 be two different hinges of locations of structures respectively, and 7 be cover plate, 8 It is substrate, 9 be sample introduction end, and 10 be terminal, and 12 be nut;The hinge-type clamp structure in legend is only the legend of signal Structure, actual its structure of the hinge-type fixture is not limited to the legend hinge-type clamp structure;It is micro- that arrow in legend indicates this Fluidic chip its in actual motion, by pressure at two ends difference drive, the flow direction of its test liquid stream.
The ultrasonic wave damper is depicted without in legend;Also the elastic clip is depicted without in legend.
Embodiment
In this case that Fig. 1 is shown embodiment, the structure of the device includes micro-fluidic chip, the knot of the micro-fluidic chip Structure includes the substrate 8 and cover plate 7 of installing bonded to each other together, and its test liquid of the micro-fluidic chip flows into sample end 9 and the sample The terminal 10 of fluid flow is located remotely from each other, the distance between the sample introduction end 9 and the terminal 10 between 3 centimetres with 10 centimetres it Between, emphasis is, its material of the substrate 8 is dimethyl silicone polymer material, and its surface of substrate 8 is the surface of primary form, The surface of the primary form its be intended to refer to the material not no by any surface chemical modification or any surface chemical modification Primary form surface, the structure of the device also includes hinge-type fixture 1, and its appearance profile likeness in form of hinge-type fixture 1 is closed Page, the hinge-type fixture 1 by two hinges 6,11 for being mutually hinged and through two hinges 6,11 one Individual fastening screw 3 and one matched with the fastening screw 3 and fastened manually with being used for of connecting together of the fastening screw 3 and The nut 12 of hand break loose is constituted, and the nut 12 is employed in this example is easier manually operated butterfly nut, butterfly Nut is commercially available, and two hinges 6,11 of the hinge-type fixture 1 are respectively mutually drawn close with its tip and clamp the micro-fluidic core Piece, the tip is positioned in the locations of structures of the neighbouring terminal 10 of the micro-fluidic chip, an institute at least in State attaching fixation in hinge to be equiped with miniature ultrasonic transducer units, this example, the miniature ultrasonic transducer units 5 are to attach to be fixed on In hinge 6, and, higher-order of oscillation electric signal transmission cable 4, one end of the higher-order of oscillation electric signal transmission cable 4 is micro- with this Type ultrasonic transducer 5 links together;The hinge-type fixture 1 facilitates the function that the device is disassembled there is provided one;This is miniature Its major function of ultrasonic transducer 5 is that in the test of micro-fluidic chip actual sample introduction, the ultrasonic wave launched using it is reduced Interfacial tension between sample solution and the inwall of its inner passage of micro-fluidic chip, can be compatible, also, utilizes institute State the distance between sample introduction end 9 and the terminal 10 and the installation position of miniature ultrasonic transducer units 5 difference and it is felt The difference on ultrasonic intensity being subject to, its interfacial tension of sample introduction end 9 described in induced synthesis and described its interfacial tension of terminal 10 Between difference, interfacial tension difference between the micro-fluidic chip two ends 9,10 can the two ends 9 of the micro-fluidic chip, Pressure gap is formed between 10, the pressure gap can drive sample solution to be flowed to the direction of terminal 10;The miniature ultrasonic Transducer 5 its function also includes checking large biological molecule contained in sample with its ultrasonic wave launched that it is micro-fluidic at this Absorption on its inner passage inner surface of chip, and then check the substrate 8 of the dimethyl silicone polymer material its body phase to the biology The effect of swallowing up of macromolecular;It is soft and have the substrate 8 of the dimethyl silicone polymer material of elasticity its function and include with it to ultrasound The property that ripple absorbs strongly, is absorbed strongly to ultrasonic wave, and thereby in the micro-fluidic chip, the terminal 10 arrives the sample introduction end The rapid decrement of ultrasonic intensity is realized within limited short distance between 9;And, ultrasonic wave damper, the ultrasonic wave disappears Subtract its profile of device in block, tabular or bar-shaped;Its material of the ultrasonic wave damper is that microcellular rubber, sponge rubber or polyurethane are micro- Hole elastomer;And, elastic clip, the elastic force accompanies two clamping limbs, medial surface and another clamping limb of each clamping limb Medial surface is relative, and the medial surface of one of clamping limb is close to the sample introduction end 9 of the micro-fluidic chip, another clamping limb it is interior The ultrasonic wave damper is close in side, and the elastic clip is with its gripping strength by the sample introduction end 9 of the micro-fluidic chip and the ultrasonic wave Damper is clipped together bonded to each otherly, the strong consumption to ultrasonic wave that the ultrasonic wave damper is brought with its material, Further support to realize that ultrasonic wave is strong within micro-fluidic chip terminal 10 to the limited short distance between the sample introduction end 9 The rapid decrement of degree, and thereby further expand between described its interfacial tension of sample introduction end 9 and the terminal 10 its interfacial tension Difference.
It is depicted without being also depicted without the elastic clip in the ultrasonic wave damper, legend in legend.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its test liquid The flow direction of stream.
Fig. 1 is depicted without the associate members such as the higher-order of oscillation electric signal generator.
Described its main body of hinge-type fixture can simply be changed a social system using commercially available hinge;It can also be customized to hardware processing factory.
Involved miniature ultrasonic transducer units 5 are commercially available;It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal transmission cable 4 is commercially available;Can also be to ultrasonic transducer producer or cable special manufacturer Family's customization.
Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available;It can also be customized to relevant manufacturers.
Its inner passage of the micro-fluidic chip involved by this case is the pipeline of hydrophobic capillary form.

Claims (6)

1. using dimethyl silicone polymer as the micro flow control chip device of substrate material, the structure of the device includes micro-fluidic chip, The structure of the micro-fluidic chip includes the substrate and cover plate of installing bonded to each other together, and its test liquid of the micro-fluidic chip flows into sample The terminal with the sample fluid flow is held to be located remotely from each other, the distance between the sample introduction end and the terminal are between 3 centimetres and 10 centimetres Between, it is characterised in that its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, The surface of the primary form its be intended to refer to the material not no by any surface chemical modification or any surface chemical modification Primary form surface, the structure of the device also includes hinge-type fixture, hinge-type fixture its appearance profile likeness in form hinge, The hinge-type fixture is by two hinges being mutually hinged and through a fastening screw of two hinges and one The nut for being used for fastening and hand break loose manually for matching and being connected together with the fastening screw with the fastening screw is constituted, the conjunction Two hinges of page fixture are respectively mutually drawn close with its tip and clamp the micro-fluidic chip, and the tip is positioned at the micro-fluidic core Fixation, which is attached, in the locations of structures of the neighbouring terminal of piece, in a hinge at least in is equiped with miniature ultrasonic Transducer, and, higher-order of oscillation electric signal transmission cable, one end and the miniature ultrasonic of the higher-order of oscillation electric signal transmission cable Wave transducer links together;The hinge-type fixture facilitates the function that the device is disassembled there is provided one;The miniature ultrasonic transducing Its major function of device be in the test of micro-fluidic chip actual sample introduction, the ultrasonic wave launched using it reduce sample solution with should Interfacial tension between the inwall of its inner passage of micro-fluidic chip, can be compatible, also, using the sample introduction end and In the distance between the terminal and the miniature ultrasonic transducer units installation position difference and its ultrasonic intensity experienced Difference, the difference between its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension, the micro-fluidic chip Interfacial tension difference between the two ends can form pressure gap between the two ends of the micro-fluidic chip, and the pressure gap can drive Sample solution is moved to the end flow;Its function of the miniature ultrasonic transducer units also includes checking examination with its ultrasonic wave launched Its absorption on the micro-fluidic chip its inner passage inner surface of contained large biological molecule in sample, and then check the poly- diformazan Swallow up effect of its body phase of the substrate of radical siloxane material to the large biological molecule;The dimethyl silicone polymer that is soft and having elasticity The substrate of material its function includes, with its property to the strong absorption of ultrasonic wave, ultrasonic wave being absorbed strongly, and thereby at this The micro-fluidic chip terminal is to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end;And, Ultrasonic wave damper, its profile of ultrasonic wave damper is in block, tabular or bar-shaped;Its material of the ultrasonic wave damper is micropore Rubber, sponge rubber or microporous polyurethane elastomer;And, elastic clip, the elastic force accompanies two clamping limbs, each clamping limb Medial surface it is relative with the medial surface of another clamping limb, the medial surface of one of clamping limb is close to entering for the micro-fluidic chip Sample end, the medial surface of another clamping limb is close to the ultrasonic wave damper, and the elastic clip is with its gripping strength by the micro-fluidic core The sample introduction end of piece and the ultrasonic wave damper are clipped together bonded to each otherly, the ultrasonic wave damper with its material brought to super The strong consumption of sound wave, further support micro-fluidic chip terminal to the limited short distance between the sample introduction end it The rapid decrement of ultrasonic intensity is inside realized, and thereby further expands described its interfacial tension of sample introduction end and its interface of the terminal Difference between tension force.
2. the micro flow control chip device according to claim 1 using dimethyl silicone polymer as substrate material, its feature exists In its material of the cover plate is dimethyl silicone polymer material.
3. the micro flow control chip device according to claim 1 using dimethyl silicone polymer as substrate material, its feature exists In described its thickness of cover plate and substrate is between 1.0 millimeters and 5.0 millimeters.
4. the micro flow control chip device according to claim 1 using dimethyl silicone polymer as substrate material, its feature exists Also include higher-order of oscillation electric signal generator in the structure of, the device, its other end of the higher-order of oscillation electric signal transmission cable with The higher-order of oscillation electric signal generator is connected.
5. the micro flow control chip device according to claim 1 using dimethyl silicone polymer as substrate material, its feature exists In its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 2 milliwatts and 9000 milliwatts, the miniature ultrasonic The frequency of its ultrasonic wave operationally launched of wave transducer is between 100KHz and 12MHz.
6. the micro flow control chip device according to claim 1 using dimethyl silicone polymer as substrate material, its feature exists In the microcellular rubber is micropore silicon rubber or micropore fluorubber.
CN201610100299.7A 2016-02-15 2016-02-15 The micro flow control chip device of substrate material is used as using dimethyl silicone polymer Pending CN107081179A (en)

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Application publication date: 20170822