CN107670711A - For the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter - Google Patents
For the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter Download PDFInfo
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- CN107670711A CN107670711A CN201610688330.3A CN201610688330A CN107670711A CN 107670711 A CN107670711 A CN 107670711A CN 201610688330 A CN201610688330 A CN 201610688330A CN 107670711 A CN107670711 A CN 107670711A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
- B01L2300/0838—Capillaries
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
- B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
- B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
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Abstract
The present invention relates to a kind of micro flow control chip device of the double mode coupling driving for hydrophobic base sheet matter, belong to analysis testing field.The substrate of micro-fluidic chip, physical presence series problem are made using dimethyl silicone polymer i.e. PDMS that is cheap and easily processing, this case is directed to the serial problem.This case main points are, the selected PDMS with ecosystem surface of substrate, and its neighbor positions fixes installing miniature ultrasonic transducer units in the sample liquid stream terminal of the micro-fluidic chip, interfacial tension is reduced with ultrasonic wave, strong absorbability using PDMS to ultrasonic wave simultaneously, reach ultrasonic intensity rapid decrement in short distance, so as to form interfacial tension difference at the both ends of the chip, the difference provides a kind of strength driven sample liquid stream and flowed along hydrophobic capillary channel to the terminal direction, the strength with Micropump its mechanicalness pumping strength included in structure while mutually support, intercouple, mutually adjustment, Collaboration.
Description
Technical field
The present invention relates to a kind of micro flow control chip device of the double mode coupling driving for hydrophobic base sheet matter, belong to point
Analyse testing field.
Background technology
For the microflow control technique overall general picture of itself, famous micro-fluidic expert Mr. Lin Ping Cheng may refer to not
The monograph " diagram Microfluid based Lab on a chip " gone out before long, the monograph is published via Science Press, and the monograph is for miniflow
Past of control technology, now, and, vision of the future etc. etc., suffer from detail, be deep into long opinion of detail
State.
The field that micro-fluidic chip is often applied to include to containing large biological molecule sample solution carry out it is various analysis,
Detection.
The basic framework of micro-fluidic chip, including be etched with the substrate of small fluid course and fit together therewith
Cover plate, the small fluid course on the substrate, before upper cover plate is assembled, it is apparent on see to be exactly some micro-channels, to wait until
After covering cover plate thereon, just really closure forms the small fluid course, the conduit inner surface of the micro-channel together with
The part cover plate that surround the micro-channel forms described small fluid course together；So, it is clear that after assembling completes
The small fluid course, the major part of its inner surface area is the inner surface area of that micro-channel, in other words, should
The state or property of micro-channel inner surface substantially determine the integrality or property of the small fluid course；Therefore say, this
The inner surface state or inner surface property of micro-channel of the individual structure on substrate are key factors；In principle, it is any can
The material of its solid forms is kept or kept substantially, can be used to make substrate and cover plate, such as, it can act as substrate and lid
The material of piece can be monocrystalline silicon piece, quartz plate, sheet glass, high polymer for example dimethyl silicone polymer, polymethyl methacrylate,
Makrolon etc.；Certainly, the selection of substrate and the selection of cover plate be able to can also be differed with identical；From material consumption, make
From the point of view of difficulty and application popularization prospect etc. etc., not small difference, the especially choosing of that substrate between these materials be present
Material, have a great influence.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, at this
It is extremely simple that micro-channel is made on the substrate of sample, and the lower cost for material, base is made with the polydimethyl siloxane material
Piece, micro-channel, and the cover plate phase made with the cheap material such as glass or polypropylene or other plastic sheets are being suppressed or etched thereon
Coordinate, be a kind of more satisfactory selection seemingly；Certainly, patch material can also select to use cheap dimethyl silicone polymer
Material：So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, and making is extremely simple, seems
It should be extremely easy to popularize, promote.
But thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that alphabetical PDMS is referred to of abridging, itself are a kind of strong
Strong hydrophobic material, micro-channel is built on this material, if without being operated for the modified of the micro-channel surface, that
, after overall assembling is completed, that is, after covering cover plate, because the micro-channel its inner surface in structure occupies most liquid
The inner surface of circulation road, then, its strong hydrophobic property of the PDMS micro-channels inner surface, is deciding factor, it can cause
Similar to the aqueous solution the fine liquid stream of polar liquid by becoming very difficult, its flow resistance is big, or even in general is micro-
Pump is all difficult to promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar；
Therefore, among prior art, modification is modified particular for the micro-channel inner surface on the PDMS material, is necessary behaviour
Make：So, this is pretty troublesome for the modified operation of PDMS micro-channel inner surfacesThat falls nor this problem, is formed tight
Weight technology puzzlement, be another problem：PDMS polymer molecules inside its body phase of this PDMS material substrate have automatic
The characteristic diffuse to the surface, migrated, the spy that this substrate body phase inside PDMS polymer molecules are diffused to the surface, migrated automatically
Property, the state after modification by its inner surface of that micro-channel of surface modifying and decorating will be caused can not to maintain long enough
Time, the holding time for micro-channel its inner surface state after that is surface-modified be substantially only sufficient to complete laboratory internal
The time of test experiments needs；In other words, by surface modification or the PDMS micro-channel inner surfaces of surface modification, it is modified
The surface state that is formed can not be lasting afterwards or after saying modification, but soon automatically tends to or say and become surface modification again
Surface state before, the strong hydrophobic surface state of that script is returned in the shorter time, then, just think, this
The micro-fluidic chip of sample can largely make, mass storage, be widely popularized, and answer is it is obvious that is, impossible.This
Micro-channel on PDMS material, if not doing surface modification, similar to the aqueous solution the fine liquid stream of polar solvent can not pump it is logical
Cross, chip also cannot just use；And if having done surface modification, its state after modifying can not be persistently kept again, or together
Sample can not popularization and application.
So, how to accomplish that substrate can either be made using cheap PDMS material, and the microflute can be released
Road inner surface decorating state can not persistently, chip can not largely make, largely lay in and then be widely popularized and such a make ability
The puzzlement that the numerous professionals in domain are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, it is stated that, its surface is strongly hydrophobic above, this strong hydrophobic
Material surface and also have another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and
And these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into it is gradually deep, until
It is heavy to be trapped within the body phase of PDMS substrates, in fact, this process, partly it is also due to PDMS material body phase interior polymer
Molecule, which has, to be diffused to the surface, caused by travel motion；Such case, it can also be explained from another angle, i.e. continue not
Disconnectedly from inside PDMS body phases to those polymer molecules of its diffusion into the surface, migration, its result moved, be little by little by that
It has been involved in a bit by the large biological molecule of adsorption within the body phase of PDMS substrates, briefly, these adsorbed biologies
Macromolecular is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up the phenomenon of large biological molecule, its institute
Caused by influence, necessarily cause the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, the problem of PDMS substrates, is, its not only adsorption large biological molecule, and swallow up biological big point
Son, so, as the large biological molecule of experiment test object, its disappearance will not stop because surface saturation is adsorbed, and
It is, it is constantly adsorbed, also constantly swallowed up.
On PDMS substrates, its body phase is constantly swallowed up and tests showing for associated biomolecule macromolecular in related experiment test process
As, it is to say that another kind, which is explained, substantial amounts of Minute pores in PDMS body phases be present, associated biomolecule macromolecular by after adsorption,
Depression enters these Minute pores, and then is swallowed up；However, inventor thinks, those can allow the sky of miniature scale
Qi leel squeezes into the Minute pores therebetween, not equal to saying that they also can directly allow the large biological molecule of relative large scale to enter
Enter, both difference on yardstick are huge, must not make sweeping generalizations.Explanation is bypassed, in any case, as dependence test analysis object
Large biological molecule is adsorbed by PDMS substrate micro-channels inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is person in charge of reception at ceremonies
Phenomenon existing for sight.
, can be from containment PDMS surfaces pair in order to prevent swallow up effect of this PDMS substrate bodies relative to large biological molecule
The absorption of large biological molecule addresses, and method is chemically modified modification aiming at the PDMS material surface, for
For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described micro-channel part, by changing
The micro-channel inner surface of modification is learned, its absorption to large biological molecule can be contained, and then avoid large biological molecule
Swallowed up by PDMS substrate body phases；But or that old problem, that is, the chemical modification on PDMS material surface is modified
Surface state afterwards can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface, move automatically
The process of shifting, it soon can become that micro-channel inner surface state being modified by surface chemical modification again script strong and dredge
Water and the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS bases
Its micro-channel inner surface of piece is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, and can is enough
Reach and contain the absorption process of the PDMS substrate micro-channel inner surfaces to large biological molecule for a long time, and then prevent PDMS substrate body phases
The effect of swallowing up to large biological molecule so that related chip manufactured goods be able to maintain that one it is prolonged enough, reasonably guarantee the quality
Phase, it is exactly a very intractable problem.The problem equally makes this area numerous as another problem addressed above
Professional is entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.
Also be present many year in the problem, so far, also not yet properly settled.
Third, as described above, the PDMS micro-channel inner surfaces are strongly hydrophobic, and targetedly surface chemical modification or surface
Chemical modification is difficult to persistently again, therefore, actually can only still belong to effective in the surface state after the modification of its surface or surface modification
It is short-term within use it；If having had been subjected to that ofer short duration period of validity, and still by force if use, due to table
Again close to hydrophobic state, bigger flowing is then already certainly existed using usual Micropump driving sample liquid stream for surface state
Resistance, so, just by increasing Micropump pump power and pumping pressure test liquid can only be forced to flow to target direction flowing, as above
Described, this PDMS material is soft, pumps sample liquid stream with too high, mechanical pumping pressure, will cause the substrate
Bubbling, expanded, distortion, deformation, also, this high pressure feelings occur for the micro-channel that its sample introduction end includes sample introduction end near zone
Under condition, the stripping between substrate and cover plate also easily occurs in the sample introduction end and its micro-channel of near zone and its periphery,
Under this situation, the crack for the appearance that sample solution will enter after the stripping between the substrate and cover plate formed and it is horizontal everywhere
Stream, this actually results in the damage of the micro-fluidic chip；It is certainly, not in place if surface modification or surface modification script,
It can cause said circumstances occur within the of short duration usual term of validity；The feelings of liquid stream driving are being carried out using additional Micropump merely
Under condition, the above-mentioned problem exists all the time.As described above, if any surface modification or surface modification etc. were not done completely
Pre action, then, the above-mentioned problem will be even more serious, is roused even without micro-channel described in sample introduction end and its near zone occurs
The problems such as being peeled off between bubble, expanded, distortion, deformation and substrate and cover plate, merely because the flow resistance is excessive, using high pressure
Micropump also may not can drive sample liquid stream to be marched forward towards terminal side.
The content of the invention
The technical problem to be solved by the invention is to provide a package solution, while solve to address above
Three aspect a series of problem actually mutually involved together.
The present invention solves the technical problem by following scheme, and the device that the program provides is that one kind is directed to hydrophobic substrate
The micro flow control chip device of the double mode coupling driving of material, the structure of the device include micro-fluidic chip, the micro-fluidic chip
Structure include being bonded to each other installing substrate and cover plate together, its test liquid of the micro-fluidic chip flows into sample end and the sample
The terminal of fluid flow is located remotely from each other, and the distance between the sample introduction end and the terminal are between 3 centimetres and 10 centimetres, the terminal
Linked together with terminal liquid pool, the profile of its structure of the terminal liquid pool is in pond shape, pit shape or cup-shaped, and the terminal liquid pool is used for
The sample solution that terminal is flow to via the micro-fluidic chip its internal pipeline is received, emphasis is that its material of the substrate is poly- two
Methylsiloxane material, its surface of the substrate are the surfaces of primary form, the surface of the primary form its be intended to refer to and do not have
By any surface chemical modification or the surface of the primary form of the material of any surface chemical modification, the structure of the device is also
Including miniature ultrasonic transducer units, and, higher-order of oscillation electric signal transmission cable, the one of the higher-order of oscillation electric signal transmission cable
End links together with the miniature ultrasonic transducer units, and the miniature ultrasonic transducer units are installed in the micro-fluidic chip with attaching
The position of the neighbouring terminal of cover plate or substrate；Its major function of the miniature ultrasonic transducer units is actual in micro-fluidic chip
When sample introduction is tested, the ultrasonic wave launched using it reduce the inwall of sample solution and its inner passage of micro-fluidic chip it
Between interfacial tension, can be compatible, also, utilize the sample introduction end and the terminal and the miniature ultrasonic transducer units
Difference in the distance between installation position difference and its ultrasonic intensity experienced, sample introduction end described in induced synthesis its
Difference between interfacial tension and the terminal its interfacial tension, the interfacial tension difference meeting between the micro-fluidic chip both ends
Pressure gap is formed between the both ends of the micro-fluidic chip, the pressure gap can drive sample solution to the terminal stream
It is dynamic；The ultrasonic wave that its function of the miniature ultrasonic transducer units also includes being launched with it checks big point of biology contained in sample
Its absorption on the micro-fluidic chip its inner passage inner surface of son, and then check the substrate of the dimethyl silicone polymer material
Swallow up effect of its body phase to the large biological molecule；It is soft and have the substrate of the dimethyl silicone polymer material of elasticity its function
Including with its property to the strong absorption of ultrasonic wave, being absorbed strongly to ultrasonic wave, and thereby at the micro-fluidic chip end
Hold the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end；And Micropump, the Micropump with
The sample introduction end connects；The function of the Micropump is, between the inwall of the sample solution and its inner passage of micro-fluidic chip
Interfacial tension is reduced, under the increased precondition of alternate compatibility by the ul-trasonic irradiation, is pumped with the mechanicalness of the Micropump
Interfacial tension difference its caused driving force between the both ends that strength induces come the ultrasonic wave is supported mutually, mutually
Mutually adjust, be mutually coupled, the power that one driving test liquid flows to the terminal direction flowing is pooled in a manner of Collaboration
Amount, some high hydroscopic resin particles are filled with the terminal liquid pool, its opening end of the terminal liquid pool is covered by breathable microporous film.
The word of Micropump one art-recognized meanings of itself are known for the professional in micro-fluidic chip field.
The Micropump both can be the Micropump of external form；The Micropump can also do into or say the embedded micro-fluidic chip
The Micropump of the built in version of its internal sample introduction end structure position or the sample introduction end Near-neighbor Structure position.
For example miniature piezoelectric pump of the Micropump, miniature peristaltic pump or miniature air driven pump.
Only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field,
It is known.
Various sizes, variously-shaped ultrasonic transducer are commercially available；Its size of commercially available miniature ultrasonic transducer units
It may diminish to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface its
It is known general technology for the professional in ultrasonic technology field for body.This case is not to this expansion superfluous words.
Only with regard to naked PDMS substrates itself micro-channel molding or lithographic technique for, be open-and-shut known skill
Art.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the device can also include higher-order of oscillation electric signal generator；The higher-order of oscillation electric signal transmission cable
Its other end can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, come for the professional in ultrasonic technology field
Say, be simple and known；The higher-order of oscillation electric signal generator can customize to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 2 milliwatts and 9000 milliwatts
Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with
Between 12MHz.
Only for the word of high hydroscopic resin one art-recognized meanings of itself, come for the professional of chemical field
Say, be known.
The high hydroscopic resin is commercially available.
Only for the word of breathable microporous film one art-recognized meanings of itself, for the professional of technical field of membrane,
It is known.
The breathable microporous film is commercially available.
This case device can further include some annexes certainly, and the annex can for example coordinate with micro-fluidic chip
The electrically or optically detection device used.The equipment that the electricity, optics etc. are used cooperatively with micro-fluidic chip, may refer to
The monograph.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, such as：Polypropylene,
Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, for example do
Into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and realized in the extremely short distance to ultrasonic wave
Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip
Using dimethyl silicone polymer it is used as the cover plate, and this case technical scheme is allowed.
Described its thickness of cover plate and substrate can allow be any setting the thickness for being easy to assembling, the thickness of recommendation or say
Preferable thickness is between 1.0 millimeters and 5.0 millimeters.Less thickness is advantageous to save material.
It is an advantage of the invention that its close position installs miniature ultrasonic with attaching in the terminal of the micro-fluidic chip
Wave transducer, it is launched using the miniature ultrasonic transducer units low-power, the ultrasonic wave of high-frequency band so that without table
Compatibility between strong hydrophobic its tube wall of micro-fluidic chip internal pipeline and the test object aqueous solution of face chemical modification
It is significantly increased, this is sample liquid stream by providing a realistic possibility；Meanwhile using dimethyl silicone polymer substrate its
To the strong absorbability of ultrasonic wave, in shorter distance, it is, from the terminal to the sample introduction end only
In the very short distance of several centimeters of yardsticks, reach the rapid decrement of ultrasonic intensity, thereby described the two of the micro-fluidic chip
The difference of the interfacial tension is caused at end, and the difference of the interfacial tension between the both ends can cause a kind of driving force, this kind because
Its function of driving force is to drive sample liquid stream in originally strongly hydrophobic capillary channel caused by interfacial tension difference
Flowed to the terminal direction；It is micro-fluidic with this in the sample solution and the simultaneous Micropump in structure, its function are
Interfacial tension between the inwall of its inner passage of chip is reduced, the increased premise bar of alternate compatibility by the ul-trasonic irradiation
Under part, its institute of interfacial tension difference for being come with the mechanicalness of Micropump pumping strength between the both ends of ultrasonic wave induction
The driving force brought is supported mutually, adjusts mutually, is mutually coupled, and one driving test liquid is pooled in a manner of Collaboration
Flow to the strength of the terminal direction flowing；The presence of the Micropump so that the miniature ultrasonic transducer units its ultrasonic wave transmitting is strong
Degree can be allowed to appropriate reduction, and this is particularly suitable for for the situation containing ultrasonic wave sensitive composition in detection object；And due to
The presence of the ultrasonic transducer and its radiated ultrasonic wave, it is possible to increase alternate compatibility, reduce interfacial tension, and provide and be somebody's turn to do
Both ends interfacial tension difference special driving force caused by it, then, in this case, sample liquid stream its in the micro-channel
Interior flow resistance is greatly reduced, and correspondingly, its running resistance of the Micropump is greatly reduced, and so, the Micropump just can be to compare
Low pumping pressure carries out the pumping work for the sample solution, because mechanical pumping pressure is greatly reduced, because
This, in such a situation, be just not susceptible to caused by sample introduction terminal tool pumping pressure is excessive the sample introduction end and its
The problems such as stripping between the micro-channel bubbling of near zone, expansion, deformation, distortion and the region substrate and cover plate etc.；
This case dual drive couples the scheme of running and added for the handling of sample fluid flow action, can allow for making
With the multiple indexs of ultrasonic intensity, ultrasonic frequency, Micropump pump power, Micropump pumping pressure etc. come the stream for the flowing
Dynamic speed, flowing action include flow forward or pause flowing or acceleration flowing etc. flowing action carry out multi-parameter and accurately grasped
Control；By this case dual drive coupling operation scheme, entirely without must be to its micro-channel of the substrate of the dimethyl silicone polymer material
Etc. relevant surfaces carry out any surface chemical modification or surface chemical modification, altogether dispensed with the surface chemical modification or table
The laborious procedures of face chemical modification；On the other hand, the ultrasonic wave of the low-power, high-frequency band, additionally it is possible to contain the life in sample
Absorption of the thing macromolecular on literalness naked its inner surface of pipeline of dimethyl silicone polymer substrate, so contain this poly- two
Swallow up effect of its body phase of methylsiloxane substrate to the large biological molecule；Due to described suction-operated and described gulp down
Do not act on and being contained, therefore, dependence test result will be better able to objectively reflect actual conditions.
Based on this case scheme, completely without carrying out coming to the surface for its relevant surfaces of dimethyl silicone polymer substrate
Modification or surface chemical modification operation are learned, therefore, this surface chemical modification layer or surface chemical modification layer not need
In the presence of, then, its body phase interior polymer molecule of the dimethyl silicone polymer substrate constantly diffuses to the surface, migrates its institute automatically
It is caused that the damaging influence of the surface chemical modification layer or surface chemical modification layer is also just not present.
It is related that the technical scheme of this case has dissolved its application to dimethyl silicone polymer substrate addressed above totally
A series of technical barriers.Based on this case scheme, this kind be very cheap and the easily dimethyl silicone polymer material of processing and fabricating
Material be just possible in the micro-fluidic chip its prepare, production, using etc. field play bigger effect.
Brief description of the drawings
Fig. 1 is its rough outside side view of this case device.
In figure, 1 is the substrate of dimethyl silicone polymer material, and 2 be cover plate, and 3 be higher-order of oscillation electric signal transmission cable, 4
It is miniature ultrasonic transducer units, 5 be the sample introduction end of the micro-fluidic chip, and 6 be the terminal of the micro-fluidic chip, and 7 are
Micropump, 8 be infusion tube；Arrow in legend indicate the micro-fluidic chip its in actual motion, it is poor by pressure at two ends
Driving, the flow direction of its sample liquid stream.
Embodiment
In this case that Fig. 1 is shown embodiment, the structure of the device includes micro-fluidic chip, the micro-fluidic chip
Structure includes being bonded to each other the substrate 1 and cover plate 2 of installing together, and its test liquid of the micro-fluidic chip flows into sample end 5 and the examination
The terminal 6 of sample fluid flow is located remotely from each other, the distance between the sample introduction end 5 and the terminal 6 between 3 centimetres and 10 centimetres,
The terminal 6 links together with terminal liquid pool, and the profile of its structure of the terminal liquid pool is in pond shape, pit shape or cup-shaped, the terminal
Liquid pool is used to receive the sample solution for flowing to terminal 6 via the micro-fluidic chip its internal pipeline, and emphasis is, the substrate 1 its
Material is dimethyl silicone polymer material, and its surface of substrate 1 is the surface of primary form, its meaning of the surface of the primary form
Refer to not by any surface chemical modification or the surface of the primary form of the material of any surface chemical modification, the dress
The structure put also includes miniature ultrasonic transducer units 4, and, higher-order of oscillation electric signal transmission cable 3, the higher-order of oscillation electric signal
One end of transmission cable 3 links together with the miniature ultrasonic transducer units 4, and the miniature ultrasonic transducer units 4 attach ground installing
The micro-fluidic chip cover plate 2 or substrate 1 the neighbouring terminal 6 position；Its main work(of the miniature ultrasonic transducer units 4
Can be in the test of micro-fluidic chip actual sample introduction, the ultrasonic wave launched using it reduces sample solution and the micro-fluidic core
Interfacial tension between the inwall of its inner passage of piece, can be compatible, also, utilizes the sample introduction end 5 and the end
Hold on the distance between 6 and the installation position of miniature ultrasonic transducer units 4 difference and its ultrasonic intensity experienced
Difference between difference, its interfacial tension of sample introduction end 5 described in induced synthesis and the terminal 6 its interfacial tension, the micro-fluidic core
Interfacial tension difference between the piece both ends 5,6 can form pressure gap between the both ends 5,6 of the micro-fluidic chip, the pressure
Power difference can drive sample solution to be flowed to the direction of terminal 6；Its function of the miniature ultrasonic transducer units 4 also includes with its institute
The ultrasonic wave of transmitting check in sample contained large biological molecule its on the micro-fluidic chip its inner passage inner surface
Absorption, and then check swallow up effect of its body phase of the substrate 1 of the dimethyl silicone polymer material to the large biological molecule；Softness is simultaneously
Its function of the substrate 1 of the dimethyl silicone polymer material of tool elasticity is included with its property to the strong absorption of ultrasonic wave, to super
Sound wave is absorbed strongly, and thereby in the micro-fluidic chip, the terminal 6 is arrived within the limited short distance between the sample introduction end 5
Realize the rapid decrement of ultrasonic intensity；And Micropump 7, the Micropump 7 are connected with the sample introduction end 5；The function of the Micropump 7 is,
Interfacial tension between the sample solution and the inwall of its inner passage of micro-fluidic chip is reduced, phase by the ul-trasonic irradiation
Between under the increased precondition of compatibility, the both ends with ultrasonic wave induction are come with the mechanicalness pumping strength of the Micropump 7
5th, interfacial tension difference its caused driving force between 6 is supported mutually, adjusts mutually, is mutually coupled, with Collaboration
Mode pool the strength that one driving test liquid flows to the direction of terminal 6 flowing, in the terminal liquid pool filled with
High hydroscopic resin particle, its opening end of the terminal liquid pool are covered by breathable microporous film.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by both ends 5,6 pressure differentials drive, its
The flow direction of sample liquid stream.
Fig. 1 is depicted without the associate members such as the higher-order of oscillation electric signal generator.
Involved miniature ultrasonic transducer units 4 are commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal transmission cable 3 is commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also determine to relevant manufacturers
System.
Involved Micropump 7 can customize to specialized factory.
Its inner passage of the micro-fluidic chip involved by this case is the pipeline of hydrophobic capillary form.
The Micropump both can be the Micropump of external form；The Micropump can also do into or say the embedded micro-fluidic chip
The Micropump of the built in version of its internal sample introduction end structure position or the sample introduction end Near-neighbor Structure position.Micropump in legend is external
The Micropump of form；The Micropump can certainly do into or say sample introduction end or its neighbour knot inside the embedded microfluidic chip structure
The Micropump of the built in version of structure position, its basic structure key element are identical with the Micropump of external form.
Claims (5)
1. the micro flow control chip device of the double mode coupling driving for hydrophobic base sheet matter, the structure of the device is including micro-fluidic
Chip, the structure of the micro-fluidic chip include being bonded to each other the substrate and cover plate of installing together, its sample of the micro-fluidic chip
Liquid stream sample introduction end and the terminal of the sample fluid flow are located remotely from each other, and the distance between the sample introduction end and the terminal are between 3 centimetres
Between 10 centimetres, the terminal links together with terminal liquid pool, the profile of its structure of the terminal liquid pool in pond shape, pit shape or
Cup-shaped, the terminal liquid pool are used to receive the sample solution for flowing to terminal via the micro-fluidic chip its internal pipeline, its feature
It is, its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the primary form
Surface its be intended to refer to the primary shape of the not material by any surface chemical modification or any surface chemical modification
The surface of state, the structure of the device also include miniature ultrasonic transducer units, and, higher-order of oscillation electric signal transmission cable, the height
Frequency vibration swings one end of electric signal transmission cable and the miniature ultrasonic transducer units link together, miniature ultrasonic transducer units patch
The position of the cover plate of the micro-fluidic chip or the neighbouring terminal of substrate is installed in attachedly；Its master of the miniature ultrasonic transducer units
It is in the test of micro-fluidic chip actual sample introduction to want function, and the ultrasonic wave launched using it reduces sample solution and the miniflow
The interfacial tension between the inwall of chip its inner passage is controlled, can be compatible, also, utilize the sample introduction end and described
In the distance between terminal and the miniature ultrasonic transducer units installation position difference and its ultrasonic intensity experienced
Difference between difference, its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension, the micro-fluidic chip
Interfacial tension difference between the both ends can form pressure gap between the both ends of the micro-fluidic chip, the pressure gap meeting
Sample solution is driven to the end flow；The ultrasonic wave that its function of the miniature ultrasonic transducer units also includes being launched with it is held back
Only its absorption on the micro-fluidic chip its inner passage inner surface of large biological molecule contained in sample, and then check this
Swallow up effect of its body phase of the substrate of dimethyl silicone polymer material to the large biological molecule；The poly- diformazan that is soft and having elasticity
Its function of the substrate of radical siloxane material includes, with its property to the strong absorption of ultrasonic wave, absorbing ultrasonic wave strongly,
And thereby realize the fast of ultrasonic intensity within micro-fluidic chip terminal to the limited short distance between the sample introduction end
Quick depletion；And Micropump, the Micropump are connected with the sample introduction end；The function of the Micropump is, in the sample solution and the micro-fluidic core
Interfacial tension between the inwall of its inner passage of piece is reduced, the increased precondition of alternate compatibility by the ul-trasonic irradiation
Under, interfacial tension difference its band for being come with the mechanicalness of Micropump pumping strength between the both ends of ultrasonic wave induction
The driving force come is supported mutually, adjusts mutually, is mutually coupled, and one driving sample liquid stream is pooled in a manner of Collaboration
The strength flowed to the terminal direction, be filled with some high hydroscopic resin particles in the terminal liquid pool, the terminal liquid pool its open
Mouth end is covered by breathable microporous film.
2. the micro flow control chip device of the double mode coupling driving according to claim 1 for hydrophobic base sheet matter, its
It is characterised by, its material of the cover plate is dimethyl silicone polymer material.
3. the micro flow control chip device of the double mode coupling driving according to claim 1 for hydrophobic base sheet matter, its
It is characterised by, described its thickness of cover plate and substrate is between 1.0 millimeters and 5.0 millimeters.
4. the micro flow control chip device of the double mode coupling driving according to claim 1 for hydrophobic base sheet matter, its
Be characterised by, the structure of the device also includes higher-order of oscillation electric signal generator, the higher-order of oscillation electric signal transmission cable its
The other end is connected with the higher-order of oscillation electric signal generator.
5. the micro flow control chip device of the double mode coupling driving according to claim 1 for hydrophobic base sheet matter, its
It is characterised by, for its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units between 2 milliwatts and 9000 milliwatts, this is miniature
The frequency of its ultrasonic wave operationally launched of ultrasonic transducer is between 100KHz and 12MHz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610688330.3A CN107670711A (en) | 2016-08-02 | 2016-08-02 | For the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter |
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| CN201610688330.3A CN107670711A (en) | 2016-08-02 | 2016-08-02 | For the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112058323A (en) * | 2019-06-10 | 2020-12-11 | 京东方科技集团股份有限公司 | Microfluidic substrate, microfluidic chip and micro total analysis system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112058323A (en) * | 2019-06-10 | 2020-12-11 | 京东方科技集团股份有限公司 | Microfluidic substrate, microfluidic chip and micro total analysis system |
| US11938481B2 (en) | 2019-06-10 | 2024-03-26 | Boe Technology Group Co., Ltd. | Microfluidic substrate, microfluidic chip and micro total analysis system |
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Application publication date: 20180209 |