CN107115900A - For the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter - Google Patents
For the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter Download PDFInfo
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- CN107115900A CN107115900A CN201610116704.4A CN201610116704A CN107115900A CN 107115900 A CN107115900 A CN 107115900A CN 201610116704 A CN201610116704 A CN 201610116704A CN 107115900 A CN107115900 A CN 107115900A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
- B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
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- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micromachines (AREA)
Abstract
The present invention relates to a kind of micro flow control chip device of the double mode coupling driving for hydrophobic base sheet matter, belong to analysis testing field.The substrate of micro-fluidic chip is made using dimethyl silicone polymer i.e. PDMS that is cheap and easily processing, physical presence series problem, this case is directed to the serial problem.This case main points are, the selected PDMS with ecosystem surface of substrate, and its neighbor positions fixes installing miniature ultrasonic transducer units in the test liquid stream terminal of the micro-fluidic chip, simultaneously, ultrasonic wave damper is installed at the sample introduction end of the micro-fluidic chip, reach ultrasonic intensity rapid decrement in short distance, so as to form interfacial tension difference at the two ends of the chip, difference offer is a kind of to drive the strength that test liquid stream flows along hydrophobic capillary channel to the terminal direction, the strength with Micropump its mechanicalness pumping strength for being included in structure while mutually support, intercouple, mutually adjustment, Collaboration.
Description
Technical field
The present invention relates to a kind of micro flow control chip device of the double mode coupling driving for hydrophobic base sheet matter, belong to analysis test
Field.
Background technology
For the microflow control technique overall general picture of itself, it may refer to famous micro-fluidic expert Mr. Lin Ping Cheng and go out not long ago
Monograph " diagram Microfluid based Lab on a chip ", the monograph is published via Science Press, and the monograph is for microflow control technique
Past, now, and, in terms of vision of the future etc., suffer from detail, be deep into long discussion of detail.
The field that micro-fluidic chip is often applied to includes carrying out various analyses, detection to the sample solution containing large biological molecule.
The basic framework of micro-fluidic chip, including it is etched with the substrate of small fluid course and the cover plate fit together therewith,
Small fluid course on the substrate, assembling upper cover plate before, it is apparent on see to be exactly some micro-channels, wait until thereon
After covering cover plate, just real closure forms the small fluid course, and the conduit inner surface of the micro-channel is together with surround this
The part cover plate of micro-channel constitutes described small fluid course together；So, it is clear that this after assembling is completed is small
Fluid course, the major part of its inner surface area is the inner surface area of that micro-channel, in other words, in the micro-channel
The state or property on surface substantially determine the integrality or property of the small fluid course；Therefore say, this is built in base
The inner surface state or inner surface property of micro-channel on piece are key factors；In principle, it is any to keep or protecting substantially
The material of its solid forms is held, can be used to make substrate and cover plate, such as, the material that can act as substrate and cover plate can be with
It is monocrystalline silicon piece, quartz plate, sheet glass, high polymer such as dimethyl silicone polymer, polymethyl methacrylate, makrolon etc.
Deng；Certainly, the selection of substrate and the selection of cover plate be able to can also be differed with identical；From material consumption, manufacture difficulty and
From the point of view of in terms of application popularization prospect etc., between these materials exist not small difference, the especially selection of that substrate, influence compared with
Greatly.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, so
Substrate on make that micro-channel is extremely simple, and the lower cost for material makes substrate with the polydimethyl siloxane material,
Micro-channel is being suppressed or etched thereon, and is being engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes,
It is a kind of more satisfactory selection seemingly；Certainly, patch material can also select to use cheap polydimethyl siloxane material：
So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, makes extremely simple, seems and should also be as
It is extremely easy to popularize, promotes.
But, thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that the letter PDMS that abridges is referred to, itself are a kind of strong
Hydrophobic material, micro-channel is built on this material, if without being operated for the modified of the micro-channel surface, then,
After overall assembling is completed, that is, cover after cover plate, because the micro-channel its inner surface in structure occupies most liquid circulation
The inner surface in road, then, its strong hydrophobic property of the PDMS micro-channels inner surface, is deciding factor, it can cause class
Be similar to the aqueous solution the fine liquid stream of polar liquid by becoming very difficult, its flow resistance is big, or even general Micropump is all
It is difficult to promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar；
Therefore, among prior art, modification is modified particular for the micro-channel inner surface on the PDMS material, is necessary
Operation；So, this is pretty troublesome for the modified operation of PDMS micro-channel inner surfacesThat falls nor this problem, structure
Perplex into serious technical, be another problem：PDMS polymer molecules tool inside its body phase of this PDMS material substrate
There are PDMS polymer molecules inside the characteristic for diffusing to the surface, migrating automatically, this substrate body phase to diffuse to the surface, move automatically
The characteristic of shifting, will cause the state after modification by its inner surface of that micro-channel of surface modifying and decorating can not maintain foot
Enough long time, the holding time for micro-channel its inner surface state after that is surface-modified is substantially only sufficient to complete in laboratory
The time of portion's test experiments needs；In other words, by surface modification or the PDMS micro-channel inner surfaces of surface modification, its
The surface state that is formed can not be lasting after modification or after saying modification, but soon automatically tend to or say and become surface again and change
Surface state before property, returns to the strong hydrophobic surface state of that script in the shorter time, then, just think,
Such micro-fluidic chip can largely make, mass storage, be widely popularized, and answer is it is obvious that is, impossible.
Micro-channel on this PDMS material, can not pump similar to the fine liquid stream of polar solvent of the aqueous solution if not doing surface modification
Send and pass through, chip also cannot just be used；And if having done surface modification, the state after its modification can not be persistently kept again, also
Being equally can not popularization and application.
So, how to accomplish that substrate can either be made using cheap PDMS material, and table in the micro-channel can be released
Face decorating state can not persistently, chip can not largely make, largely lay in so that be widely popularized it is such a make this area it is numerous specially
The puzzlement that industry personnel are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, above it is stated that, its surface is strongly hydrophobic, this strong hydrophobic
Material surface and also another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and
And, these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into gradually deep, directly
It is trapped in heavy within the body phase of PDMS substrates, in fact, this process is partly also due to inside PDMS material body phase
Polymer molecule, which has, to be diffused to the surface, caused by travel motion；Such case, can also be explained from another angle, i.e.
Continuously from inside PDMS body phases to its diffusion into the surface, migration those polymer molecules, its result moved, be by
Gradually those are involved within the body phase of PDMS substrates by the large biological molecule of adsorption, briefly, these are inhaled
Attached large biological molecule is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up large biological molecule
Phenomenon, the influence caused by it necessarily causes the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, be the problem of PDMS substrates, its not only adsorption large biological molecule, and swallow up large biological molecule,
So, as the large biological molecule of experiment test object, its disappearance will not stop because the absorption of surface saturation, but, no
It is disconnected adsorbed, also constantly swallowed up.
On PDMS substrates in related experiment test process its body phase constantly swallow up test associated biomolecule macromolecular phenomenon, separately
It is to say that one kind, which is explained, there are substantial amounts of Minute pores in PDMS body phases, and associated biomolecule macromolecular is by after adsorption, depression
Into these Minute pores, and then swallowed up；However, inventor thinks, those can allow the air point of miniature scale
Son squeezes into the Minute pores therebetween, and being not equal to them also can directly allow that the large biological molecule of relative large scale enters, and two
Person's difference on yardstick is huge, must not make sweeping generalizations.Explanation is bypassed, in any case, the biology of object is analyzed as dependence test
Macromolecular is adsorbed by PDMS substrate micro-channels inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective
The phenomenon of presence.
, can be from containment PDMS surfaces to life in order to prevent this PDMS substrate bodies relative to the effect of swallowing up of large biological molecule
The absorption of thing macromolecular is addressed, and method is chemically modified modification aiming at the PDMS material surface, for
For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described micro-channel part, by changing
The micro-channel inner surface of modification is learned, its absorption to large biological molecule can be contained, and then avoid large biological molecule quilt
PDMS substrate body phases are swallowed up；But, or that old problem, that is, the chemical modification on PDMS material surface changes
Surface state after property can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface automatically,
The process of migration, soon can become that micro-channel inner surface state being modified by surface chemical modification again script strong and dredge
Water and the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS
Its micro-channel inner surface of substrate is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, can reach again
Growth stage contains absorption process of the PDMS substrate micro-channel inner surfaces to large biological molecule, and then prevents PDMS substrate body phases
The effect of swallowing up to large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life,
It is exactly a very intractable problem.The problem makes the numerous specialties in this area as another problem addressed above, equally
Personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.The hardly possible
Also be present many year in topic, so far, also not yet properly settled.
Third, as described above, the PDMS micro-channel inner surfaces are strongly hydrophobic, and targetedly surface chemical modification or come to the surface
Learn modification to be difficult to again persistently, therefore, actually can only still belong to effective short in the surface state after the modification of its surface or surface modification
It is used within phase；If have passed through that ofer short duration period of validity, and still by force if use, due to surface shape
State already again close to hydrophobic state, is then certainly existed than larger flow resistance using usual Micropump driving test liquid stream,
So, just test liquid can only be forced to flow to target direction flowing by increasing Micropump pump power and pumping pressure, as described above,
This PDMS material is soft, pumps test liquid stream with too high, machinery pumping pressure, causing the substrate, it enters
Bubbling, expanded, distortion, deformation, also, this high-pressure situations occur for the micro-channel that sample end includes sample introduction end near zone
Under, also easily occur the stripping between substrate and cover plate, this feelings in the sample introduction end and its micro-channel of near zone and its periphery
Under shape, sample solution flows in the crack of the appearance between the substrate and cover plate that are formed after the entrance stripping everywhere, this reality
Border causes the damage of the micro-fluidic chip；Certainly, it is not in place if surface modification or surface modification script, also result in
There is said circumstances within the of short duration usual term of validity；It is above-mentioned in the case where carrying out liquid stream driving using additional Micropump merely
The problem exists all the time.If be modified or the pre action such as surface modification as described above, not doing any surface completely,
So, the above-mentioned problem will be even more serious, even without occur micro-channel bubbling described in sample introduction end and its near zone, it is expanded,
Between distortion, deformation and substrate and cover plate the problems such as peel off, merely because the flow resistance is excessive, using high pressure Micropump also not
Test liquid stream must can be driven to be marched forward towards terminal side.
The content of the invention
The technical problems to be solved by the invention are to provide a package solution, while solving three addressed above
The a series of problem actually mutually involved together of aspect.
The present invention solves the technical problem by following scheme, and the device that the program is provided is a kind of for hydrophobic base sheet matter
Double mode coupling driving micro flow control chip device, the structure of the device includes micro-fluidic chip, the structure of the micro-fluidic chip
Including the substrate and cover plate installing bonded to each other together, its test liquid of the micro-fluidic chip flows into sample end and the sample fluid flow
Terminal be located remotely from each other, the distance between the sample introduction end and the terminal are between 3 centimetres and 10 centimetres, and emphasis is, the base
Its material of piece is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, its meaning of the surface of the primary form
Think to refer to not by any surface chemical modification or the surface of the primary form of the material of any surface chemical modification, the dress
The structure put also includes miniature ultrasonic transducer units, and, higher-order of oscillation electric signal transmission cable, the higher-order of oscillation electric signal is passed
One end of transmission cable links together with the miniature ultrasonic transducer units, and the miniature ultrasonic transducer units are installed in the miniflow with attaching
Control the position of the cover plate of chip or the neighbouring terminal of substrate；Its major function of the miniature ultrasonic transducer units is in micro-fluidic core
During the test of piece actual sample introduction, the ultrasonic wave launched using it reduce sample solution with its inner passage of the micro-fluidic chip
Interfacial tension between wall, can be compatible, also, is changed using the sample introduction end and the terminal with the miniature ultrasonic
Difference in the distance between energy device installation position difference and its ultrasonic intensity experienced, sample introduction end described in induced synthesis
Interfacial tension difference meeting between difference between its interfacial tension and the terminal its interfacial tension, the micro-fluidic chip two ends
Pressure gap is formed between the two ends of the micro-fluidic chip, the pressure gap can drive sample solution to the end flow；
The miniature ultrasonic transducer units its functions also include with its ultrasonic wave launched check large biological molecule contained in sample its
Absorption on the micro-fluidic chip its inner passage inner surface, and then check the substrate of the dimethyl silicone polymer material its body phase
The effect of swallowing up to the large biological molecule；Softness simultaneously has its function of the substrate of the dimethyl silicone polymer material of elasticity including with it
To the property of the strong absorption of ultrasonic wave, ultrasonic wave is absorbed strongly, and thereby in micro-fluidic chip terminal to the sample introduction
The rapid decrement of ultrasonic intensity is realized within limited short distance between end；And, Micropump, the Micropump connects with the sample introduction end
Connect；The function of the Micropump is that the interfacial tension between the inwall of the sample solution and its inner passage of micro-fluidic chip is by this
Ul-trasonic irradiation and reduce, under the increased precondition of alternate compatibility, come and the ultrasound with the mechanicalness pumping strength of the Micropump
Interfacial tension difference its driving force brought between the two ends of ripple induction is supported mutually, adjusts mutually, is mutually coupled,
The strength that one driving test liquid flows to the terminal direction flowing is pooled in the way of Collaboration；The cover plate and substrate its
Thickness is between 1.0 millimeters and 5.0 millimeters；And, ultrasonic wave damper, its profile of the ultrasonic wave damper in it is block,
Tabular is bar-shaped；Its material of the ultrasonic wave damper is microcellular rubber, sponge rubber or microporous polyurethane elastomer；And, bullet
Power is pressed from both sides, and the elastic force accompanies two clamping limbs, and the medial surface of each clamping limb is relative with the medial surface of another clamping limb, wherein one
The medial surface of individual clamping limb is close to the sample introduction end of the micro-fluidic chip, and the medial surface of another clamping limb is close to the ultrasonic wave and disappeared
Subtract device, the elastic clip with its gripping strength by the sample introduction end of the micro-fluidic chip and the ultrasonic wave damper it is bonded to each other be clipped in one
Rise, the strong consumption to ultrasonic wave that the ultrasonic wave damper is brought with its material, further support in the micro-fluidic core
The piece terminal thereby further expands to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end
Difference between its interfacial tension of big described its interfacial tension of sample introduction end and the terminal.
The word of Micropump one art-recognized meanings of itself are known for the professional in micro-fluidic chip field.
The Micropump both can be the Micropump of external form；The Micropump can also be done into or say inside the embedded micro-fluidic chip
The Micropump of the built in version of its sample introduction end structure position or the sample introduction end Near-neighbor Structure position.
The Micropump such as miniature piezoelectric pump, miniature peristaltic pump or miniature air driven pump.
It is public only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field
Know.
Various sizes, variously-shaped ultrasonic transducer are commercially available；Commercially available miniature ultrasonic transducer units its sizes can be with
It is small to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface itself and
Speech, is known general technology for the professional in ultrasonic technology field.This case is not to this expansion superfluous words.
Only with regard to naked PDMS substrates itself micro-channel molding or lithographic technique for, be open-and-shut known technology.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the device can also include higher-order of oscillation electric signal generator；Its is another for the higher-order of oscillation electric signal transmission cable
One end can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, for the professional in ultrasonic technology field, be
It is simple and known；The higher-order of oscillation electric signal generator can be customized to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units be between 2 milliwatts and 9000 milliwatts it
Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with
Between 12MHz.
This case device can further include some annexes certainly, and the annex can for example be used cooperatively with micro-fluidic chip
Electrically or optically detection device.The equipment that the electricity, optics etc. are used cooperatively with micro-fluidic chip, may refer to described
Monograph.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, for example：Polypropylene, glass
Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, such as do
Realized into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and in the extremely short distance to ultrasonic wave
Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip
It is used as the cover plate using dimethyl silicone polymer, is also that this case technical scheme is allowed.
Described its thickness of cover plate and substrate can allow be any setting be easy to assembling thickness, the thickness of recommendation or say preferably
Thickness be between 1.0 millimeters and 5.0 millimeters.Less thickness is conducive to saving material.
Described its size of ultrasonic wave damper is not limited, and described its length of ultrasonic wave damper, width, thickness may each be according to need
The arbitrary size to be set.
It is known in field of rubber technology only for the word of microcellular rubber one art-recognized meanings of itself.
The microcellular rubber is commercially available.
It is known in field of rubber technology only for the word of sponge rubber one art-recognized meanings of itself.
The sponge rubber is commercially available.
It is known in technical field of macromolecules only for the word of microporous polyurethane elastomer one art-recognized meanings of itself.
The polyurethane micropore elastomer material is commercially available.
The microcellular rubber preferred microporous silicon rubber or micropore fluorubber.
It is known in field of rubber technology only for the word of micropore silicon rubber one art-recognized meanings of itself.
The microporous silicon Rubber market is on sale.
It is known in field of rubber technology only for the word of micropore fluorubber one art-recognized meanings of itself.
The micropore fluorubber is commercially available.
The word of elastic clip one art-recognized meanings of itself are known.
The elastic clip is commercially available.
Changed it is an advantage of the invention that installing miniature ultrasonic in its close position attaching of the terminal of the micro-fluidic chip
Can device, it is launched using the miniature ultrasonic transducer units low-power, the ultrasonic wave of high-frequency band so that without coming to the surface
The compatibility learned between strong hydrophobic its tube wall of micro-fluidic chip internal pipeline and the test object aqueous solution of modification significantly increases
Plus, this is test liquid stream by there is provided a realistic possibility；Meanwhile, using dimethyl silicone polymer substrate its to ultrasound
The strong absorbability of ripple, in shorter distance, it is, from the terminal to only several centimeters the sample introduction end
In the very short distance of yardstick, reach the rapid decrement of ultrasonic intensity, thereby cause institute at the two ends of the micro-fluidic chip
The difference of interfacial tension is stated, the difference of the interfacial tension between the two ends can cause a kind of driving force, and this kind is poor because of interfacial tension
Its function of driving force is to drive test liquid stream in strong hydrophobic capillary channel originally to the terminal side caused by different
To flowing；And the simultaneous Micropump in structure, its function is, leads in the sample solution and the micro-fluidic chip inside it
Interfacial tension between the inwall in road is reduced by the ul-trasonic irradiation, under the increased precondition of alternate compatibility, with the Micropump
The two ends that are induced come the ultrasonic wave of mechanicalness pumping strength between interfacial tension difference its driving force for being brought
Support mutually, adjust mutually, be mutually coupled, one driving test liquid is pooled in the way of Collaboration and flows to the terminal side
To the strength of flowing；The presence of the Micropump so that its ultrasonic wave emissive porwer of the miniature ultrasonic transducer units can be allowed to appropriateness
Reduction, this is particularly suitable for for the situation containing ultrasonic wave sensitive composition in detection object；And due to the ultrasonic transducer and its
The presence of radiated ultrasonic wave, it is possible to increase alternate compatibility, reduce interfacial tension, and provide the two ends interfacial tension difference its
The special driving force brought, then, in this case, its flow resistance in the micro-channel of test liquid stream significantly drops
Low, correspondingly, its running resistance of the Micropump is greatly reduced, so, and the Micropump just can be to carry out than relatively low pumping pressure
For the pumping work of the sample solution, because mechanical pumping pressure is greatly reduced, therefore, in such a situation,
Just be not susceptible to because sample introduction terminal tool pumping pressure it is excessive caused by the sample introduction end and its near zone the micro-channel drum
Between bubble, expansion, deformation, distortion and the region substrate and cover plate the problems such as stripping etc.；This case dual drive coupling running
Scheme and add for the sample fluid flow act it is handling, can allow for using ultrasonic intensity, ultrasonic frequency,
Micropump pump power, Micropump pumping pressure etc. multiple indexs include flowing forward to be directed to the flow rate of the flowing, flowing action
Dynamic or pause flowing or acceleration flowing etc. flowing action carries out multi-parameter and accurately manipulated；By this case dual drive coupling running side
Case, is repaiied entirely without that must carry out any surface chemistry to the substrate of the dimethyl silicone polymer material its micro-channel etc. relevant surfaces
Decorations or surface chemical modification, have altogether dispensed with the laborious procedures of the surface chemical modification or surface chemical modification；On the other hand, should
The ultrasonic wave of low-power, high-frequency band, additionally it is possible to contain the large biological molecule in sample in the literalness naked poly dimethyl silicon
Absorption on its inner surface of pipeline of oxyalkyl piece, and then contain its body phase of the dimethyl silicone polymer substrate to the large biological molecule
The effect of swallowing up；Because described suction-operated and the described effect of swallowing up are contained that therefore, dependence test result will more
Actual conditions can objectively be reflected.
This case its installation position is located at the ultrasonic wave damper at the sample introduction end, from its terminal of this case micro-fluidic chip to entering
Said on the sport technique segment that ultrasonic intensity rapid decrement is realized in the such short distance in sample end, the ultrasonic wave damper its function as
The size expansion device of micro-fluidic chip, in other words, the ultrasonic wave damper its function as significantly extend it is micro-fluidic
Its terminal of chip the distance between to sample introduction end, be the equal of from length of several centimetres of the extension of script to more than ten centimetres or
Twenties centimetres of length or longer length, so so that described terminal is strong to the ultrasonic wave between sample introduction end in short distance
The sport technique segment of degree rapid decrement can relatively easily be reached, also, the size expansion device namely the ultrasonic wave damper its
Installation and removal are all very convenient, it is important that it can also be reused.
Its presence of the ultrasonic wave damper in this case device, further reduce to its pumping pressure of Micropump and pump power according to
Rely；Correspondingly, its running resistance of the Micropump is further greatly reduced, so, and the Micropump just can be with lower pumping
Pressure carries out the pumping work for the sample solution, because mechanical pumping pressure is further greatly reduced,
Therefore, in such a situation, just be less susceptible to occur because sample introduction terminal tool pumping pressure it is excessive caused by the sample introduction end and
The problems such as stripping between the micro-channel bubbling of its near zone, expansion, deformation, distortion and the region substrate and cover plate etc.；
The scheme of this case dual drive coupling running is further added for the test liquid due to its presence of the ultrasonic wave damper
The handling of flowing action is flowed, wherein, the factor that can adjust includes ultrasonic intensity, ultrasonic frequency, Micropump pump power, micro-
Pump pumps pressure, and, the selection and use of ultrasonic wave damper material, the selection of ultrasonic wave damper block size and use,
The selection of the size of its corresponding ultrasonic wave abatement ability of ultrasonic wave damper and use etc., this kind of many adjustable factor it is same
When exist, can more operation indexs, further, more it is multifactor adjustably for the flowing flow rate, stream
Action includes flow forward or pause flowing or accelerates flowing etc. flowing action to carry out the accurate manipulation under more parameters.
Based on this case scheme, repaiied completely without the surface chemistry carried out for the dimethyl silicone polymer substrate its relevant surfaces
Decorations or surface chemical modification operation, therefore, this surface chemical modification layer or surface chemical modification layer not need to exist,
So, its body phase interior polymer molecule of the dimethyl silicone polymer substrate is constantly diffused to the surface, migrated caused by it automatically
Damaging influence to surface chemical modification layer or surface chemical modification layer is also just not present.
The technical scheme of this case has dissolved its application related one to dimethyl silicone polymer substrate addressed above totally
Row technical barrier.Based on this case scheme, the polydimethyl siloxane material of this kind very cheap and easily processing and fabricating just has can
Can in the micro-fluidic chip its prepare, production, using etc. field play bigger effect.
Brief description of the drawings
Fig. 1 is its rough outside side view of this case device.
In figure, 1 is the substrate of dimethyl silicone polymer material, and 2 be cover plate, and 3 be higher-order of oscillation electric signal transmission cable, 4
It is miniature ultrasonic transducer units, 5 be the sample introduction end of the micro-fluidic chip, and 6 be the terminal of the micro-fluidic chip, 7
It is Micropump, 8 be infusion tube；Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends
Difference driving, the flow direction of its test liquid stream.
Embodiment
In this case that Fig. 1 is shown embodiment, the structure of the device includes micro-fluidic chip, the knot of the micro-fluidic chip
Structure includes the substrate 1 and cover plate 2 of installing bonded to each other together, and its test liquid of the micro-fluidic chip flows into sample end 5 and the sample
The terminal 6 of fluid flow is located remotely from each other, the distance between the sample introduction end 5 and the terminal 6 between 3 centimetres and 10 centimetres,
Emphasis is, its material of the substrate 1 is dimethyl silicone polymer material, and its surface of substrate 1 is the surface of primary form, the original
The surface of raw form its be intended to refer to not by any surface chemical modification or the original of the material of any surface chemical modification
The surface of raw form, the structure of the device also includes miniature ultrasonic transducer units 4, and, higher-order of oscillation electric signal transmission cable
3, one end of the higher-order of oscillation electric signal transmission cable 3 links together with the miniature ultrasonic transducer units 4, the miniature ultrasonic
Wave transducer 4 is installed in the position of the cover plate 2 of the micro-fluidic chip or the neighbouring terminal 6 of substrate 1 with attaching；This is micro-
Its major function of type ultrasonic transducer 4 is that in the test of micro-fluidic chip actual sample introduction, the ultrasonic wave launched using it is dropped
Interfacial tension between low sample solution and the inwall of its inner passage of micro-fluidic chip, can be compatible, also, utilizes
The distance between the sample introduction end 5 and the terminal 6 and the installation position of miniature ultrasonic transducer units 4 difference and its felt
The difference on ultrasonic intensity being subject to, its interfacial tension of sample introduction end 5 described in induced synthesis and described its interfacial tension of terminal 6 it
Between difference, the interfacial tension difference between the micro-fluidic chip two ends 5,6 can be at the two ends 5,6 of the micro-fluidic chip
Between form pressure gap, the pressure gap can drive sample solution to be flowed to the direction of terminal 6；The miniature ultrasonic transducing
Device 4 its function also include with its ultrasonic wave launched check large biological molecule contained in sample its in the micro-fluidic chip
Absorption on its inner passage inner surface, so check its body phase of the substrate 1 of the dimethyl silicone polymer material to the biology greatly point
The effect of swallowing up of son；Softness simultaneously has its function of the substrate 1 of the dimethyl silicone polymer material of elasticity including strong to ultrasonic wave with it
The property of strong absorption, is absorbed strongly to ultrasonic wave, and thereby between micro-fluidic chip terminal 6 to the sample introduction end 5
Limited short distance within realize the rapid decrement of ultrasonic intensity；And, Micropump 7, the Micropump 7 connects with the sample introduction end 5
Connect；The function of the Micropump 7 is, the interfacial tension between the inwall of the sample solution and its inner passage of micro-fluidic chip by
The ul-trasonic irradiation and reduce, under the increased precondition of alternate compatibility, come and this with the mechanicalness pumping strength of the Micropump 7
Interfacial tension difference its driving force brought between the two ends 5,6 of ultrasonic wave induction supports mutually, mutually adjustment,
It is mutually coupled, the strength that one driving test liquid flows to the direction of terminal 6 flowing is pooled in the way of Collaboration；It is described
Cover plate 2 and substrate 1 its thickness are between 1.0 millimeters and 5.0 millimeters；And, ultrasonic wave damper, the ultrasonic wave disappears
Subtract its profile of device in block, tabular or bar-shaped；Its material of the ultrasonic wave damper is that microcellular rubber, sponge rubber or polyurethane are micro-
Hole elastomer；And, elastic clip, the elastic force accompanies two clamping limbs, medial surface and another clamping limb of each clamping limb
Medial surface is relative, and the medial surface of one of clamping limb is close to the sample introduction end 5 of the micro-fluidic chip, another clamping limb it is interior
The ultrasonic wave damper is close in side, and the elastic clip is with its gripping strength by the sample introduction end 5 of the micro-fluidic chip and the ultrasonic wave
Damper is clipped together bonded to each otherly, the strong consumption to ultrasonic wave that the ultrasonic wave damper is brought with its material,
Further support to realize ultrasonic intensity within micro-fluidic chip terminal 6 to the limited short distance between the sample introduction end 5
Rapid decrement, and thereby further expand the difference between described its interfacial tension of sample introduction end 5 and the terminal 6 its interfacial tension
It is different.
The ultrasonic wave damper is depicted without in legend；Also the elastic clip is depicted without in legend.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by two ends 5,6 pressure differentials drive, its try
The flow direction of sample liquid stream.
Fig. 1 is depicted without the associate members such as the higher-order of oscillation electric signal generator.
Involved miniature ultrasonic transducer units 4 are commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal transmission cable 3 is commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also be customized to relevant manufacturers.
Involved Micropump 7 can be customized to specialized factory.
Its connected mode with various micro-fluidic chip of various Micropump is known.
Its inner passage of the micro-fluidic chip involved by this case is the pipeline of hydrophobic capillary form.
The Micropump both can be the Micropump of external form；The Micropump can also be done into or say inside the embedded micro-fluidic chip
The Micropump of the built in version of its sample introduction end structure position or the sample introduction end Near-neighbor Structure position, its basic structure key element and external form
Micropump it is identical.Micropump in legend is the Micropump of external form.
Claims (5)
1. the micro flow control chip device of the double mode coupling driving for hydrophobic base sheet matter, the structure of the device includes micro-fluidic core
Piece, the structure of the micro-fluidic chip includes the substrate and cover plate of installing bonded to each other together, its test liquid stream of the micro-fluidic chip
Sample introduction end and the terminal of the sample fluid flow are located remotely from each other, and the distance between the sample introduction end and the terminal are between 3 centimetres and 10
Between centimetre, it is characterised in that its material of the substrate is dimethyl silicone polymer material, its surface of the substrate is primary form
Surface, the surface of the primary form its be intended to refer to not by any surface chemical modification or any surface chemical modification should
The surface of the primary form of material, the structure of the device also includes miniature ultrasonic transducer units, and, higher-order of oscillation electric signal is passed
Transmission cable, one end and the miniature ultrasonic transducer units of the higher-order of oscillation electric signal transmission cable link together, the miniature ultrasonic
Wave transducer is installed in the position of the cover plate of the micro-fluidic chip or the neighbouring terminal of substrate with attaching；The miniature ultrasonic is changed
Can device its major function be in the actual sample introduction test of micro-fluidic chip, the ultrasonic wave launched using it reduce sample solution and
Interfacial tension between the inwall of its inner passage of micro-fluidic chip, can be compatible, also, using the sample introduction end with
And the distance between the terminal and the miniature ultrasonic transducer units installation position difference and its ultrasonic intensity experienced
On difference, the difference between its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension, the micro-fluidic core
Interfacial tension difference between the piece two ends can form pressure gap between the two ends of the micro-fluidic chip, the pressure gap meeting
Sample solution is driven to the end flow；Its function of the miniature ultrasonic transducer units also includes checking with its ultrasonic wave launched
Its absorption on the micro-fluidic chip its inner passage inner surface of contained large biological molecule in sample, so check this poly- two
Swallow up effect of its body phase of the substrate of methylsiloxane material to the large biological molecule；The polydimethylsiloxanes that are soft and having elasticity
The substrate of alkane material its function includes, with its property to the strong absorption of ultrasonic wave, ultrasonic wave being absorbed strongly, and thereby exists
The micro-fluidic chip terminal is to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end；With
And, Micropump, the Micropump is connected with the sample introduction end；The function of the Micropump is, in the sample solution and the micro-fluidic chip inside it
Interfacial tension between the inwall of passage is reduced by the ul-trasonic irradiation, under the increased precondition of alternate compatibility, micro- with this
Its driving force for being brought of interfacial tension difference between the two ends that are induced come the ultrasonic wave of mechanicalness pumping strength of pump
Amount is supported mutually, adjusts mutually, is mutually coupled, and one driving test liquid is pooled in the way of Collaboration and flows to the terminal
The strength of direction flowing；Described its thickness of cover plate and substrate is between 1.0 millimeters and 5.0 millimeters；And, ultrasonic wave disappears
Subtract device, its profile of ultrasonic wave damper is in block, tabular or bar-shaped；Its material of the ultrasonic wave damper is microcellular rubber, sea
Continuous rubber or microporous polyurethane elastomer；And, elastic clip, the elastic force accompanies two clamping limbs, the medial surface of each clamping limb
Relative with the medial surface of another clamping limb, the medial surface of one of clamping limb is close to the sample introduction end of the micro-fluidic chip, separately
The medial surface of one clamping limb is close to the ultrasonic wave damper, and the elastic clip is with its gripping strength by the sample introduction of the micro-fluidic chip
End with the ultrasonic wave damper it is bonded to each other clip together, the ultrasonic wave damper with its material brought to the strong of ultrasonic wave
Strong consumption, further supports that realization is super within micro-fluidic chip terminal to the limited short distance between the sample introduction end
The rapid decrement of intensity of acoustic wave, and thereby further expand between described its interfacial tension of sample introduction end and the terminal its interfacial tension
Difference.
2. the micro flow control chip device of the double mode coupling driving according to claim 1 for hydrophobic base sheet matter, it is special
Levy and be, its material of the cover plate is dimethyl silicone polymer material.
3. the micro flow control chip device of the double mode coupling driving according to claim 1 for hydrophobic base sheet matter, it is special
Levy and be, the microcellular rubber is micropore silicon rubber or micropore fluorubber.
4. the micro flow control chip device of the double mode coupling driving according to claim 1 for hydrophobic base sheet matter, it is special
Levy and be, the structure of the device also includes higher-order of oscillation electric signal generator, its is another for the higher-order of oscillation electric signal transmission cable
End is connected with the higher-order of oscillation electric signal generator.
5. the micro flow control chip device of the double mode coupling driving according to claim 1 for hydrophobic base sheet matter, it is special
Levy and be, its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 2 milliwatts and 9000 milliwatts, and this is miniature
The frequency of its ultrasonic wave operationally launched of ultrasonic transducer is between 100KHz and 12MHz.
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| CN201610116704.4A CN107115900A (en) | 2016-02-25 | 2016-02-25 | For the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter |
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| CN201610116704.4A CN107115900A (en) | 2016-02-25 | 2016-02-25 | For the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter |
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