CN106556711A - For the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter - Google Patents

Abstract

The present invention relates to a kind of micro flow control chip device of the double mode coupling driving for hydrophobic base sheet matter, belongs to analysis field tests.The substrate of micro-fluidic chip is made using polydimethylsiloxane i.e. PDMS that is cheap and easily processing, a physical presence series difficult problem, this case are directed to the serial difficult problem.This case main points are, substrate selectes the PDMS with ecosystem surface, and test liquid stream terminal its neighbor positions in the micro-fluidic chip fix installing miniature ultrasonic transducer units, interfacial tension is reduced with ultrasound wave, strong absorbabilities of the PDMS to ultrasound wave is utilized simultaneously, reach ultrasonic intensity rapid decrement in the short distance, so as to form interfacial tension difference at the two ends of the chip, the difference provide it is a kind of drive test liquid stream along strength from hydrophobic capillary channel to terminal direction flowing, the strength and while its mechanicalness pumping strength is mutually supported with the Micropump that includes in structure, intercouple, mutually adjust, Collaboration.

Description

For the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter

Technical field

The present invention relates to a kind of micro flow control chip device of the double mode coupling driving for hydrophobic base sheet matter, belongs to analysis test Field.

Background technology

For the microflow control technique overall general picture of itself, may refer to famous micro-fluidic expert Mr. Lin Ping Cheng and go out not long ago Monograph " diagram Microfluid based Lab on a chip ", the monograph via Science Press publish, the monograph is for microflow control technique Past, now, and, vision of the future etc. aspect, suffer from detail, be deep into long discussion of detail.

The field that micro-fluidic chip is often applied to includes carrying out various analyses, detection to the sample solution containing biomacromolecule.

The basic framework of micro-fluidic chip, including the substrate for being etched with small fluid course and the cover plate for fitting together therewith, Small fluid course on the substrate, before assembling upper cover plate, it is apparent on see to be exactly some micro-channel, be when thereon After covering cover plate, just real closure forms the small fluid course, and the conduit inner surface of the micro-channel is together with surround this The part cover plate of micro-channel constitutes described small fluid course together；So, it is clear that this after assembling is completed is small Fluid course, the major part of its inner surface area is the inner surface area of that micro-channel, in other words, in the micro-channel The state or property on surface substantially determines the integrality or property of the small fluid course；Therefore say, this structure is in base The inner surface state or inner surface property of the micro-channel on piece is key factor；In principle, it is any to keep or protecting substantially The material of its solid forms is held, can be used to make substrate and cover plate, such as, the material that can act as substrate and cover plate can be with It is monocrystalline silicon piece, piezoid, sheet glass, high polymer such as polydimethylsiloxane, polymethyl methacrylate, Merlon etc. Deng；Certainly, the selection of substrate can be with identical with the selection of cover plate, it is also possible to differs；From material consumption, manufacture difficulty and Application popularization prospect etc. aspect from the point of view of, there is not little difference between these materials, especially the selection of that substrate, affect compared with Greatly.

In various substrate making materials, polydimethylsiloxane, i.e. PDMS, comparatively very easy molding, so Substrate on make that micro-channel is extremely simple, and the lower cost for material makes substrate with the polydimethyl siloxane material, Suppress or etch micro-channel thereon, and the cover plate made with glass or the cheap material such as polypropylene or other plastic sheets is engaged, It is a kind of more satisfactory selection seemingly；Certainly, patch material can also be selected using cheap polydimethyl siloxane material： So, scheme of this substrate selection for polydimethyl siloxane material, material are extremely cheap, make extremely simple, seem and should also be as It is extremely easy to popularize, promotes.

But, thing is really not so simple.

First, this polydimethyl siloxane material, that is, the material referred to by the letter PDMS that abridges, itself are a kind of strong Hydrophobic material, builds micro-channel on this material, if not carrying out the modified operation for the micro-channel surface, then, Integral installation with after completing, that is, after covering cover plate, because of structure in the micro-channel its inner surface occupy most liquid circulation The inner surface in road, then, the PDMS micro-channel inner surface its strong hydrophobic property is deciding factor, and it can cause class Be similar to aqueous solution the fine liquid stream of polar liquid by becoming very difficult, its flow resistance is big, or even general Micropump is all It is difficult to promote, certainly, if cover plate is also selected using the PDMS material, then, problem is substantially identical, similar； Therefore, among prior art, modification is modified particular for the micro-channel inner surface on the PDMS material, is necessary Operation；So, this is pretty troublesome for the modified operation of PDMS micro-channel inner surfaciesThat falls nor this problem, structure Into serious technical puzzlement, it is another problem：PDMS polymer molecules tool inside this PDMS material substrate its body phase There is the characteristic for diffusing to the surface automatically, migrating, PDMS polymer molecules are diffused to the surface automatically, moved inside this substrate body phase The characteristic of shifting, by cause that micro-channel its inner surface through surface modifying and decorating it is modified after state can not maintain foot The enough long time, being held time of the micro-channel after that is surface-modified its inner surface state be substantially only sufficient to, and to complete experiment indoor The time of portion's test experiments needs；In other words, PDMS micro-channel inner surfacies of or surface modification modified through surface, its The apparent condition formed after modified or after saying modification can not be lasting, but soon automatically tends to or say become surface again and change Apparent condition before property, returns to the strong hydrophobic apparent condition of that script in the shorter time, then, just think, Such micro-fluidic chip can be made in a large number, mass storage, be widely popularized, and answer is it is obvious that is, impossible. Micro-channel on this PDMS material, if not doing surface modification, cannot pump similar to the fine liquid stream of polar solvent of aqueous solution Send and pass through, chip also cannot just be used；And if having done surface modification, the state after its modification cannot be persistently kept again, also Being equally cannot popularization and application.

So, how to accomplish substrate can either be made using cheap PDMS material, and can release table in the micro-channel Face decorating state cannot persistently, chip cannot make in a large number, deposit and then be widely popularized and such a make this area numerous specially in a large number The puzzlement that industry personnel are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not be despised.

Be present many days in the highly difficult problem, so far, not yet properly settled.

Second, the PDMS material of non-surface modification, above it is stated that, its surface is strongly hydrophobic, this strong hydrophobic Material surface and there is another problem, that is, biomacromolecule can be adsorbed in this strong hydrophobic PDMS surfaces, and And, the depression that these adsorbed biomacromolecules can also be further further on PDMS surfaces gradually falls into gradually deep, directly It is trapped within the body phase of PDMS substrates, in fact to heavy, this process is partly also due to inside PDMS material body phase Polymer molecule have diffuse to the surface, travel motion is caused；Such case, it is also possible to explain from another angle, i.e. Continuously from inside PDMS body phases to its diffusion into the surface, migration those polymer molecules, its motion result, be by Gradually those are involved within the body phase of PDMS substrates, briefly by the biomacromolecule of surface adsorption, these are inhaled Attached biomacromolecule is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up biomacromolecule Phenomenon, the impact caused by which, the severe deviations of all kinds of test data of experiment for necessarily causing to be related to biomacromolecule.

As described above, the problem of PDMS substrates is, its not only surface adsorption biomacromolecule, and swallow up biomacromolecule, So, as experiment test object biomacromolecule its disappear will not because surface saturation adsorb and stop, but, no It is disconnected adsorbed, also constantly swallowed up.

With regard to PDMS substrates in related experiment test process its body phase constantly swallow up test associated biomolecule macromole phenomenon, separately A kind of explanation is to say, there are substantial amounts of Minute pores in PDMS body phases, associated biomolecule macromole by after surface adsorption, depression Into these Minute pores, and then swallowed up；However, inventor thinks, those can allow the air point of miniature scale Son clamp-ons the Minute pores therebetween, is not equal to them and also can directly allow that the biomacromolecule of relative large scale is entered, and two Person's difference on yardstick is huge, must not make sweeping generalizations.Explanation is bypassed, and in any case, the biology of object is analyzed as dependence test Macromole is adsorbed by PDMS substrate micro-channel inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective The phenomenon of presence.

In order to prevent this PDMS substrate bodies relative to the effect of swallowing up of biomacromolecule, can be from containment PDMS surfaces opposite The absorption of thing macromole addressing, method be aiming at the PDMS material surface be chemically modified it is modified, for For PDMS is for the situation of substrate material, modified, Jing Guohua is chemically modified to the surface of described micro-channel part exactly The micro-channel inner surface of modification is learned, its absorption to biomacromolecule can be contained, and then avoided biomacromolecule quilt PDMS substrate body phases are swallowed up；But, or that old problem, that is, the chemical modification on PDMS material surface changes Apparent condition after property persistently cannot keep, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface automatically, The process of migration, soon can become that again script strong through the micro-channel inner surface state that surface chemical modification is modified and dredge The state of water and strong adsorption biomacromolecule, in other words, no matter how professionals in the field turn from side to side, the PDMS Substrate its micro-channel inner surface is always rapidly to strong hydrophobic surface state evolution.

So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, can reach again Growth stage contains the absorption process of the PDMS substrate micro-channel inner surfacies to biomacromolecule, and then prevents PDMS substrate body phases The effect of swallowing up to biomacromolecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life, It is exactly a very thorny difficult problem.The difficult problem makes the numerous specialties in this area as another difficult problem addressed above, equally Personnel are entangled with for a long time, perplex, and the difficult problem is equally the highly difficult problem that its obvious technology barrier can not be despised.The hardly possible Also be present many days in topic, so far, also not yet properly settled.

Third, as described above, the PDMS micro-channel inner surfacies are strongly hydrophobic, and targetedly surface chemical modification or come to the surface Learn modification to be again difficult to persistently, therefore, actually can only its surface is modified or surface modification after apparent condition still belong to effectively short It is used within phase；If have passed through that ofer short duration expiry date, and still by force if use, due to surface shape State already again near hydrophobic state, drives test liquid stream then to certainly exist than larger flow resistance using usual Micropump, So, test liquid just can only be forced by increasing Micropump pump power and pumping pressure to flow to target direction flowing, as described above, This PDMS material is soft, pumps test liquid stream with the pumping pressure of too high, machinery, and which enters will to cause the substrate Sample end includes that the micro-channel of sample introduction end near zone occurs bubbling, expanded, distortion, deformation, also, this high-pressure situations Under, the micro-channel and its periphery in the sample introduction end and its near zone is also susceptible to the stripping between substrate and cover plate, this feelings Under shape, the crack of the appearance between the substrate formed into after the stripping and cover plate is flow over by sample solution everywhere, this reality Border causes the damage of the micro-fluidic chip；Certainly, if the surface is modified or surface modification is not in place if originally, also result in There is said circumstances within of short duration usual effect duration；It is in the case where liquid stream driving is carried out using additional Micropump merely, above-mentioned The problem is present all the time.If as described above, do not did that any surface is modified completely or the pre action such as surface modification, So, the above-mentioned problem will be even more serious, even without occur sample introduction end and its micro-channel bubbling described near zone, it is expanded, Between distortion, deformation and substrate and cover plate the problems such as peel off, merely because the flow resistance is excessive, using high pressure Micropump also not Test liquid stream must be driven to march forward towards terminal side.

The content of the invention

The technical problem to be solved is to provide a package solution, while solving three for addressing above The a series of difficult problem for actually mutually involving together of aspect.

The present invention solves the technical problem by following scheme, and the device that the program is provided is a kind of for hydrophobic base sheet matter Double mode coupling driving micro flow control chip device, the structure of the device includes micro-fluidic chip, the structure of the micro-fluidic chip Including the substrate installed together bonded to each other and cover plate, its test liquid stream sample introduction end of the micro-fluidic chip and the sample fluid flow Terminal be located remotely from each other, the distance between the sample introduction end and the terminal between 3 centimetres and 10 centimetres, it is important that, the base Piece its material is polydimethylsiloxane material, and its surface of the substrate is the surface of primary form, its meaning of surface of the primary form Think of refer to not through any surface chemical modification or any surface chemical modification the material primary form surface, the dress The structure put also includes miniature ultrasonic transducer units, and, higher-order of oscillation electric signal transmission cable, the higher-order of oscillation electric signal are passed One end of transmission cable is linked together with the miniature ultrasonic transducer units, and the miniature ultrasonic transducer units are installed in the miniflow with attaching The position of the neighbouring described terminal of the cover plate or substrate of control chip；The miniature ultrasonic transducer units its major functions is in micro-fluidic core When the actual sample introduction of piece is tested, the ultrasound wave launched using which is reducing in sample solution and its inner passage of the micro-fluidic chip Interfacial tension between wall so as to can be compatible, also, changed with the miniature ultrasonic using the sample introduction end and the terminal Difference that can be on the distance between device installation position difference and its ultrasonic intensity for being experienced, sample introduction end described in induced synthesis Difference between its interfacial tension and the terminal its interfacial tension, the interfacial tension difference meeting between the micro-fluidic chip two ends Pressure gap is formed between the two ends of the micro-fluidic chip, the pressure gap can drive sample solution to the end flow； The miniature ultrasonic transducer units its functions also include with its ultrasound wave launched check biomacromolecule contained in sample its Absorption on the micro-fluidic chip its inner passage inner surface, and then check substrate its body phase of the polydimethylsiloxane material The effect of swallowing up to the biomacromolecule；Substrate its function of the polydimethylsiloxane material that is soft and having elasticity is included with which Property to the strong absorption of ultrasound wave, is absorbed strongly to ultrasound wave, and thereby in the micro-fluidic chip terminal to the sample introduction The rapid decrement of ultrasonic intensity is realized within limited short distance between end；And, Micropump, the Micropump are connected with the sample introduction end Connect；The function of the Micropump is that the interfacial tension between the inwall of the sample solution and its inner passage of micro-fluidic chip is received should Ul-trasonic irradiation and reduce, under the precondition that the alternate compatibility increases, come and the ultrasound with the mechanicalness of Micropump pumping strength Interfacial tension difference its driving force brought between the two ends of ripple induction is supported mutually, adjusts mutually, is mutually coupled, One is pooled in the way of Collaboration drives test liquid to flow to the strength of the terminal direction flowing.

One word of Micropump art-recognized meanings of itself are known for the professional in micro-fluidic chip field.

The Micropump can both be the Micropump of external form；The Micropump can also be done into or say inside the embedded micro-fluidic chip The Micropump of the built in version of its sample introduction end structure position or the sample introduction end Near-neighbor Structure position.

For example miniature piezoelectric pump of the Micropump, miniature peristaltic pump or miniature air driven pump.

For one word of the ultrasonic transducer art-recognized meanings of itself of professional only with regard to to(for) ultrasonic technology field, it is public Know.

Various sizes, variously-shaped ultrasonic transducer are commercially available；Commercially available miniature ultrasonic transducer units its sizes can be with It is little to only with millimeter calculate magnitude.

Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface itself and Speech, for the professional in ultrasonic technology field, is known general technology.This case is not to this expansion superfluous words.

Only with regard to naked PDMS substrates itself micro-channel molding or lithographic technique for, be open-and-shut known technology.

The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.

The structure of the device can also include higher-order of oscillation electric signal generator；Which is another for the higher-order of oscillation electric signal transmission cable One end can be connected with the higher-order of oscillation electric signal generator.

The involved higher-order of oscillation electric signal generator technology of itself, for the professional in ultrasonic technology field, be It is simple and known；The higher-order of oscillation electric signal generator can be customized to ultrasonic instrument specialized factory.

The preferred scope of the miniature ultrasonic transducer units its specified ultrasonic emitting power be between 2 milliwatts and 9000 milliwatts it Between；The preferred scope of the frequency of the miniature ultrasonic transducer units its ultrasound wave operationally launched be between 100KHz with Between 12MHz.

This case device can further include some adnexaes certainly, and the adnexa for example can be used cooperatively with micro-fluidic chip Electrically or optically testing equipment.The equipment that the electricity, optics etc. are used cooperatively with micro-fluidic chip, may refer to described Monograph.

The cover plate in chip structure, its material can allow to be any electrical insulating property material, for example：Polypropylene, glass Glass, polymethyl methacrylate, polydimethylsiloxane, etc., in order to make smaller size of micro-fluidic chip, such as do Into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and realize to ultrasound wave in the extremely short distance Extremely fast decay, it may be preferred to polydimethylsiloxane is used as cover plate.Certainly, select on large-sized micro-fluidic chip It is used as the cover plate using polydimethylsiloxane, and this case technical scheme is allowed.

The cover plate and substrate its thickness can allow the thickness for being easy to assemble for being any setting, the thickness of recommendation or say preferably Thickness be between 1.0 millimeters and 5.0 millimeters.Less thickness is conducive to material-saving.

It is an advantage of the invention that the terminal its close position in the micro-fluidic chip is installed miniature ultrasonic with attaching and is changed Can device, which is launched using the miniature ultrasonic transducer units low-power, the ultrasound wave of high-frequency band so that without coming to the surface The compatibility learned between modified strong hydrophobic its tube wall of micro-fluidic chip internal pipeline and test object aqueous solution significantly increases Plus, this is test liquid stream by there is provided a realistic possibility；Meanwhile, using polydimethylsiloxane substrate its to ultrasound The strong absorbability of ripple, in shorter distance, it is, from the terminal to the only several centimeters the sample introduction end In the very short distance of yardstick, the rapid decrement of ultrasonic intensity is reached, thereby institute is caused at the two ends of the micro-fluidic chip The difference of interfacial tension is stated, the difference of the interfacial tension between the two ends can cause a kind of driving force, and this kind is poor because of interfacial tension Different and caused driving force its function is to drive test liquid stream in strong hydrophobic capillary channel originally to the terminal side To flowing；And the simultaneous Micropump in structure, its function is, leads to inside which in the sample solution and the micro-fluidic chip Interfacial tension between the inwall in road receive the ul-trasonic irradiation and reduce, the alternate compatibility increase precondition under, with the Micropump Mechanicalness pumping strength come the interfacial tension difference between the two ends of the ultrasound wave induction driving force brought by which Support mutually, adjust mutually, be mutually coupled, one is pooled in the way of Collaboration and drives test liquid to flow to the terminal side To the strength of flowing；The presence of the Micropump so that the miniature ultrasonic transducer units its ultrasonic emitting intensity can be allowed to appropriateness Reduce, this is particularly suitable for for the situation containing ultrasound wave sensitive composition in detection object；And due to the ultrasonic transducer and its The presence of radiated ultrasound wave, it is possible to increase the alternate compatibility, reduces interfacial tension, and provide two ends interfacial tensions difference its The special driving force brought, then, in this case, test liquid stream its flow resistance in the micro-channel significantly drops Low, correspondingly, the Micropump its running resistance is greatly reduced, and so, the Micropump just can be carrying out than relatively low pumping pressure For the pumping work of the sample solution, as mechanical pumping pressure is greatly reduced, therefore, in such a situation, The micro-channel drum because of the excessive and caused sample introduction end of sample introduction terminal tool pumping pressure and its near zone is not susceptible to just Between bubble, expansion, deformation, distortion and the region substrate and cover plate the problems such as stripping etc.；This case Dual Drive coupling running Scheme and increased for the handling of the sample fluid flow action, can allow for using ultrasonic intensity, ultrasonic frequency, Micropump pump power, Micropump pump pressure etc. multiple indexs to include flowing forward for the flow rate of the flowing, flowing action Dynamic or time-out flowing or acceleration flowing etc. flowing action carries out multiparameter and accurately manipulates；By this case, the Dual Drive couples running side Case, repaiies entirely without carrying out any surface chemistry to substrate its micro-channel of the polydimethylsiloxane material etc. relevant surfaces Decorations or surface chemical modification, have altogether dispensed with the laborious procedures of the surface chemical modification or surface chemical modification；On the other hand, should The ultrasound wave of low-power, high-frequency band, additionally it is possible to contain the biomacromolecule in sample in the literalness naked poly dimethyl silicon Absorption on oxyalkyl piece its inner surface of pipeline, and then contain the polydimethylsiloxane substrate its body phase to the biomacromolecule The effect of swallowing up；As described adsorption and the described effect of swallowing up are contained, therefore, dependence test result will more Practical situation can objectively be reflected.

Based on this case scheme, repair completely without the surface chemistry carried out for the polydimethylsiloxane substrate its relevant surfaces Decorations or surface chemical modification operation, therefore, this surface chemical modification layer or surface chemical modification layer not need presence, So, its body phase interior polymer molecule of the polydimethylsiloxane substrate constantly diffuse to the surface automatically, migrate its institute caused by The damaging influence of the surface chemical modification layer or surface chemical modification layer is not also just present.

The technical scheme of this case has dissolved its application related one to polydimethylsiloxane substrate addressed above totally Row technical barrier.Based on this case scheme, the polydimethyl siloxane material of this kind very cheap and easily processing and fabricating just has can Can in the micro-fluidic chip, which be prepared, production, application etc. field play bigger effect.

Description of the drawings

Fig. 1 is its rough outside side view of this case device.

In figure, 1 is the substrate of polydimethylsiloxane material, and 2 is cover plate, and 3 is higher-order of oscillation electric signal transmission cable, 4 It is miniature ultrasonic transducer units, 5 is the sample introduction end of the micro-fluidic chip, 6 is the terminal of the micro-fluidic chip, 7 It is Micropump, 8 is infusion tube；Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends Difference drives, the flow direction of its test liquid stream.

Specific embodiment

In this case that Fig. 1 is shown embodiment, the structure of the device includes micro-fluidic chip, the knot of the micro-fluidic chip Structure includes the substrate 1 installed together bonded to each other and cover plate 2, its test liquid stream sample introduction end 5 of the micro-fluidic chip and the sample The terminal 6 of fluid flow is located remotely from each other, the distance between the sample introduction end 5 and the terminal 6 between 3 centimetres and 10 centimetres, It is important that, the substrate 1 its material is polydimethylsiloxane material, and its surface of substrate 1 is the surface of primary form, the original The surface of raw form its be intended to refer to not through any surface chemical modification or the original of the material of any surface chemical modification The surface of raw form, the structure of the device also include miniature ultrasonic transducer units 4, and, higher-order of oscillation electric signal transmission cable 3, one end of the higher-order of oscillation electric signal transmission cable 3 is linked together with the miniature ultrasonic transducer units 4, the miniature ultrasonic Wave transducer 4 is installed in the position of the neighbouring described terminal 6 of the cover plate 2 or substrate 1 of the micro-fluidic chip with attaching；This is micro- Type ultrasonic transducer 4 its major function is that, when the actual sample introduction of micro-fluidic chip is tested, the ultrasound wave launched using which is dropping Interfacial tension between the inwall of low sample solution and its inner passage of micro-fluidic chip so as to can be compatible, also, utilize The distance between the sample introduction end 5 and the terminal 6 and 4 installation position of miniature ultrasonic transducer units difference and its felt The difference on ultrasonic intensity being subject to, its interfacial tension of sample introduction end 5 described in induced synthesis and the terminal 6 its interfacial tension it Between difference, the interfacial tension difference between the micro-fluidic chip two ends 5,6 can be at the two ends 5,6 of the micro-fluidic chip Between form pressure gap, the pressure gap can drive sample solution to 6 direction of the terminal flow；The miniature ultrasonic transducing Device 4 its function also include with its ultrasound wave launched check biomacromolecule contained in sample its in the micro-fluidic chip Absorption on its inner passage inner surface, and then check its body phase of substrate 1 of the polydimethylsiloxane material to the biology greatly point The effect of swallowing up of son；Substrate 1 its function of the polydimethylsiloxane material that is soft and having elasticity includes strong to ultrasound wave with which The property of strong absorption, is absorbed strongly to ultrasound wave, and thereby between micro-fluidic chip terminal 6 to the sample introduction end 5 Limited short distance within realize the rapid decrement of ultrasonic intensity；And, Micropump 7, the Micropump 7 are connected with the sample introduction end 5 Connect；The function of the Micropump 7 is that the interfacial tension between the inwall of the sample solution and its inner passage of micro-fluidic chip is received The ul-trasonic irradiation and reduce, under the precondition that the alternate compatibility increases, come and this with the mechanicalness of the Micropump 7 pumping strength Interfacial tension difference its driving force brought between the two ends 5,6 of ultrasound wave induction supported mutually, mutually adjustment, It is mutually coupled, one is pooled in the way of Collaboration and drives test liquid to flow to the strength of 6 direction of terminal flowing.

Arrow in legend indicate the micro-fluidic chip its in actual motion, by two ends 5,6 pressure differentials drive, its examination The flow direction of sample liquid stream.

Fig. 1 is depicted without the associate members such as the higher-order of oscillation electric signal generator.

Involved miniature ultrasonic transducer units 4 are commercially available；Can also customize to ultrasonic transducer producer.

Involved higher-order of oscillation electric signal transmission cable 3 is commercially available；Can also customize to ultrasonic transducer producer.

Involved higher-order of oscillation electric signal generator market has the product for being close to needs commercially available；Can also customize to relevant manufacturers.

Involved Micropump 7 can be customized to specialized factory.

Its inner passage of the micro-fluidic chip involved by this case is the pipeline of hydrophobic capillary tube form.

The Micropump can both be the Micropump of external form；The Micropump can also be done into or say inside the embedded micro-fluidic chip The Micropump of the built in version of its sample introduction end structure position or the sample introduction end Near-neighbor Structure position.Micropump in legend is external form Micropump；The Micropump can certainly do into or say sample introduction end or its Near-neighbor Structure position inside the embedded microfluidic chip structure The Micropump of the built in version, its basic structure key element are identical with the Micropump of external form.

Claims (5)

1. the micro flow control chip device of the double mode coupling driving of hydrophobic base sheet matter is directed to, and the structure of the device includes micro-fluidic core Piece, the structure of the micro-fluidic chip include installing bonded to each other substrate together and cover plate, the micro-fluidic chip its test liquid stream Sample introduction end is located remotely from each other with the terminal of the sample fluid flow, and the distance between the sample introduction end and the terminal are between 3 centimetres and 10 Between centimetre, it is characterised in that the substrate its material is polydimethylsiloxane material, and its surface of the substrate is primary form Surface, the surface of the primary form its be intended to refer to not through any surface chemical modification or any surface chemical modification should The surface of the primary form of material, the structure of the device also include miniature ultrasonic transducer units, and, higher-order of oscillation electric signal is passed Transmission cable, one end and the miniature ultrasonic transducer units of the higher-order of oscillation electric signal transmission cable link together, the miniature ultrasonic Wave transducer is installed in the position of the neighbouring described terminal of the cover plate or substrate of the micro-fluidic chip with attaching；The miniature ultrasonic is changed Can device its major function be when the actual sample introduction of micro-fluidic chip is tested, the ultrasound wave launched using which reduce sample solution and Interfacial tension between the inwall of its inner passage of micro-fluidic chip so as to can be compatible, also, using the sample introduction end with And the distance between the terminal and miniature ultrasonic transducer units installation position difference and its ultrasonic intensity for being experienced On difference, the difference between its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension, the micro-fluidic core Interfacial tension difference between the piece two ends can form pressure gap between the two ends of the micro-fluidic chip, the pressure gap meeting Sample solution is driven to the end flow；The miniature ultrasonic transducer units its functions also includes checking with its ultrasound wave launched Its absorption on the micro-fluidic chip its inner passage inner surface of contained biomacromolecule in sample, so check this poly- two Swallow up effect of its body phase of the substrate of methylsiloxane material to the biomacromolecule；The polydimethylsiloxanes that are soft and having elasticity The substrate of alkane material its function includes, with its property to the strong absorption of ultrasound wave, absorbing strongly ultrasound wave, and thereby existing The micro-fluidic chip terminal realizes the rapid decrement of ultrasonic intensity within the limited short distance between the sample introduction end；With And, Micropump, the Micropump are connected with the sample introduction end；The function of the Micropump is, in the sample solution and the micro-fluidic chip inside which Interfacial tension between the inwall of passage receive the ul-trasonic irradiation and reduce, the alternate compatibility increase precondition under, it is micro- with this The mechanicalness pumping strength of pump come the interfacial tension difference between the two ends of the ultrasound wave induction driving force brought by which Amount is supported mutually, adjusts mutually, is mutually coupled, and pools one and drive test liquid to flow to the terminal in the way of Collaboration The strength of direction flowing.

2. the micro flow control chip device of the double mode coupling driving for hydrophobic base sheet matter according to claim 1, which is special Levy and be, its material of the cover plate is polydimethylsiloxane material.

3. the micro flow control chip device of the double mode coupling driving for hydrophobic base sheet matter according to claim 1, which is special Levy and be, the cover plate and substrate its thickness are between 1.0 millimeters and 5.0 millimeters.

4. the micro flow control chip device of the double mode coupling driving for hydrophobic base sheet matter according to claim 1, which is special Levy and be, the structure of the device also includes higher-order of oscillation electric signal generator, which is another for the higher-order of oscillation electric signal transmission cable End is connected with the higher-order of oscillation electric signal generator.

5. the micro flow control chip device of the double mode coupling driving for hydrophobic base sheet matter according to claim 1, which is special Levy and be, between 2 milliwatts and 9000 milliwatts, this is miniature for the miniature ultrasonic transducer units its specified ultrasonic emitting power The frequency of ultrasonic transducer its ultrasound wave operationally launched is between 100KHz and 12MHz.