CN107185603A - Multichannel micro-fluidic device is used in a kind of strong hydrophobic cholera diagnosis of substrate of its chip - Google Patents
Multichannel micro-fluidic device is used in a kind of strong hydrophobic cholera diagnosis of substrate of its chip Download PDFInfo
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- CN107185603A CN107185603A CN201610176666.1A CN201610176666A CN107185603A CN 107185603 A CN107185603 A CN 107185603A CN 201610176666 A CN201610176666 A CN 201610176666A CN 107185603 A CN107185603 A CN 107185603A
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
- B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
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- G01N2333/183—Flaviviridae, e.g. pestivirus, mucosal disease virus, bovine viral diarrhoea virus, classical swine fever virus (hog cholera virus) or border disease virus
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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Abstract
The present invention relates to a kind of strong hydrophobic cholera diagnosis multichannel micro-fluidic device of substrate of its chip, belong to analysis testing field.The cheap and dimethyl silicone polymer i.e. PDMS that easily processes as being used for making the substrate of cholera diagnosis micro-fluidic chip if there is series problem；This case is intended to solve the serial problem totally.This case main points are, the selected PDMS with ecosystem surface of substrate, and fastening is positioned at the test liquid stream terminal of the micro-fluidic chip its neighbor positions manually by the hinge-type fixture for being attached to miniature ultrasonic transducer units, simultaneously, ultrasonic wave damper is installed at the sample introduction end of the micro-fluidic chip, reach ultrasonic intensity rapid decrement in short distance, so as to form interfacial tension difference at the two ends of the chip, difference offer is a kind of to drive the strength that test liquid stream flows along hydrophobic capillary channel to the terminal direction, the strength with Micropump its mechanicalness pumping strength for being included in structure while intercouple, Collaboration.
Description
Technical field
The present invention relates to a kind of strong hydrophobic cholera diagnosis multichannel micro-fluidic device of substrate of its chip, belong to analysis test neck
Domain.
Background technology
The micro-fluidic cholera diagnostic techniques background of associated multi-channel, may refer to the grade applications for a patent for invention of CN 200910150930.4
Case.
Only for the microflow control technique overall general picture of itself, it may refer to famous micro-fluidic expert Mr. Lin Ping Cheng and go out not long ago
Monograph " diagram Microfluid based Lab on a chip ", the monograph is published via Science Press, and the monograph is to microflow control technique
Past, now, and, in terms of vision of the future etc., suffer from detail, be deep into long discussion of detail.
So, the Important Problems of this case that to have a talk below concern.
The basic framework of micro-fluidic chip, including it is etched with the substrate of small fluid course and the cover plate fit together therewith,
Small fluid course on the substrate, assembling upper cover plate before, it is apparent on see to be exactly some micro-channels, wait until thereon
After covering cover plate, just real closure forms the small fluid course, and the conduit inner surface of the micro-channel is together with surround this
The part cover plate of micro-channel constitutes described small fluid course together；So, it is clear that this after assembling is completed is small
Fluid course, the major part of its inner surface area is the inner surface area of that micro-channel, in other words, in the micro-channel
The state or property on surface substantially determine the integrality or property of the small fluid course；Therefore say, this is built in base
The inner surface state or inner surface property of micro-channel on piece are key factors；In principle, it is any to keep or protecting substantially
The material of its solid forms is held, can be used to make substrate and cover plate, such as, the material that can act as substrate and cover plate can be with
It is monocrystalline silicon piece, quartz plate, sheet glass, high polymer such as dimethyl silicone polymer, polymethyl methacrylate, makrolon etc.
Deng；Certainly, the selection of substrate and the selection of cover plate be able to can also be differed with identical；From material consumption, manufacture difficulty and
From the point of view of in terms of application popularization prospect etc., between these materials exist not small difference, the especially selection of that substrate, influence compared with
Greatly.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, so
Substrate on make that micro-channel is extremely simple, and the lower cost for material makes substrate with the polydimethyl siloxane material,
Micro-channel is being suppressed or etched thereon, and is being engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes,
It is a kind of more satisfactory selection seemingly；Certainly, patch material can also select to use cheap polydimethyl siloxane material：
So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, makes extremely simple, seems and should also be as
It is extremely easy to popularize, promotes.
But, thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that the letter PDMS that abridges is referred to, itself are a kind of strong
Hydrophobic material, micro-channel is built on this material, if without being operated for the modified of the micro-channel surface, then,
After overall assembling is completed, that is, cover after cover plate, because the micro-channel its inner surface in structure occupies most liquid circulation
The inner surface in road, then, its strong hydrophobic property of the PDMS micro-channels inner surface, is deciding factor, it can cause class
Be similar to the aqueous solution the fine liquid stream of polar liquid by becoming very difficult, its flow resistance is big, or even general Micropump is all
It is difficult to promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar；
Therefore, among prior art, modification is modified particular for the micro-channel inner surface on the PDMS material, is necessary
Operation；So, this is pretty troublesome for the modified operation of PDMS micro-channel inner surfacesThat falls nor this problem, structure
Perplex into serious technical, be another problem：PDMS polymer molecules tool inside its body phase of this PDMS material substrate
There are PDMS polymer molecules inside the characteristic for diffusing to the surface, migrating automatically, this substrate body phase to diffuse to the surface, move automatically
The characteristic of shifting, will cause the state after modification by its inner surface of that micro-channel of surface modifying and decorating can not maintain foot
Enough long time, the holding time for micro-channel its inner surface state after that is surface-modified is substantially only sufficient to complete in laboratory
The time of portion's test experiments needs；In other words, by surface modification or the PDMS micro-channel inner surfaces of surface modification, its
The surface state that is formed can not be lasting after modification or after saying modification, but soon automatically tend to or say and become surface again and change
Surface state before property, returns to the strong hydrophobic surface state of that script in the shorter time, then, just think,
Such micro-fluidic chip can largely make, mass storage, be widely popularized, and answer is it is obvious that is, impossible.
Micro-channel on this PDMS material, can not pump similar to the fine liquid stream of polar solvent of the aqueous solution if not doing surface modification
Send and pass through, chip also cannot just be used；And if having done surface modification, the state after its modification can not be persistently kept again, also
Being equally can not popularization and application.
So, how to accomplish that substrate can either be made using cheap PDMS material, and table in the micro-channel can be released
Face decorating state can not persistently, chip can not largely make, largely lay in so that be widely popularized it is such a make this area it is numerous specially
The puzzlement that industry personnel are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, above it is stated that, its surface is strongly hydrophobic, this strong hydrophobic
Material surface and also another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and
And, these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into gradually deep, directly
It is trapped in heavy within the body phase of PDMS substrates, in fact, this process is partly also due to inside PDMS material body phase
Polymer molecule, which has, to be diffused to the surface, caused by travel motion；Such case, can also be explained from another angle, i.e.
Continuously from inside PDMS body phases to its diffusion into the surface, migration those polymer molecules, its result moved, be by
Gradually those are involved within the body phase of PDMS substrates by the large biological molecule of adsorption, briefly, these are inhaled
Attached large biological molecule is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up large biological molecule
Phenomenon, the influence caused by it necessarily causes the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, be the problem of PDMS substrates, its not only adsorption large biological molecule, and swallow up large biological molecule,
So, as the large biological molecule of experiment test object, its disappearance will not stop because the absorption of surface saturation, but, no
It is disconnected adsorbed, also constantly swallowed up.
On PDMS substrates in related experiment test process its body phase constantly swallow up test associated biomolecule macromolecular phenomenon, separately
It is to say that one kind, which is explained, there are substantial amounts of Minute pores in PDMS body phases, and associated biomolecule macromolecular is by after adsorption, depression
Into these Minute pores, and then swallowed up；However, inventor thinks, those can allow the air point of miniature scale
Son squeezes into the Minute pores therebetween, and being not equal to them also can directly allow that the large biological molecule of relative large scale enters, and two
Person's difference on yardstick is huge, must not make sweeping generalizations.Explanation is bypassed, in any case, the biology of object is analyzed as dependence test
Macromolecular is adsorbed by PDMS substrate micro-channels inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective
The phenomenon of presence.
, can be from containment PDMS surfaces to life in order to prevent this PDMS substrate bodies relative to the effect of swallowing up of large biological molecule
The absorption of thing macromolecular is addressed, and method is chemically modified modification aiming at the PDMS material surface, for
For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described micro-channel part, by changing
The micro-channel inner surface of modification is learned, its absorption to large biological molecule can be contained, and then avoid large biological molecule quilt
PDMS substrate body phases are swallowed up；But, or that old problem, that is, the chemical modification on PDMS material surface changes
Surface state after property can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface automatically,
The process of migration, soon can become that micro-channel inner surface state being modified by surface chemical modification again script strong and dredge
Water and the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS
Its micro-channel inner surface of substrate is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, can reach again
Growth stage contains absorption process of the PDMS substrate micro-channel inner surfaces to large biological molecule, and then prevents PDMS substrate body phases
The effect of swallowing up to large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life,
It is exactly a very intractable problem.The problem makes the numerous specialties in this area as another problem addressed above, equally
Personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.The hardly possible
Also be present many year in topic, so far, also not yet properly settled.
Third, as described above, the PDMS micro-channel inner surfaces are strongly hydrophobic, and targetedly surface chemical modification or come to the surface
Learn modification to be difficult to again persistently, therefore, actually can only still belong to effective short in the surface state after the modification of its surface or surface modification
It is used within phase；If have passed through that ofer short duration period of validity, and still by force if use, due to surface shape
State already again close to hydrophobic state, is then certainly existed than larger flow resistance using usual Micropump driving test liquid stream,
So, just test liquid can only be forced to flow to target direction flowing by increasing Micropump pump power and pumping pressure, as described above,
This PDMS material is soft, pumps test liquid stream with too high, machinery pumping pressure, causing the substrate, it enters
Bubbling, expanded, distortion, deformation, also, this high-pressure situations occur for the micro-channel that sample end includes sample introduction end near zone
Under, also easily occur the stripping between substrate and cover plate, this feelings in the sample introduction end and its micro-channel of near zone and its periphery
Under shape, sample solution flows in the crack of the appearance between the substrate and cover plate that are formed after the entrance stripping everywhere, this reality
Border causes the damage of the micro-fluidic chip；Certainly, it is not in place if surface modification or surface modification script, also result in
There is said circumstances within the of short duration usual term of validity；It is above-mentioned in the case where carrying out liquid stream driving using additional Micropump merely
The problem exists all the time.If be modified or the pre action such as surface modification as described above, not doing any surface completely,
So, the above-mentioned problem will be even more serious, even without occur micro-channel bubbling described in sample introduction end and its near zone, it is expanded,
Between distortion, deformation and substrate and cover plate the problems such as peel off, merely because the flow resistance is excessive, using high pressure Micropump also not
Test liquid stream must can be driven to be marched forward towards terminal side.
The content of the invention
The technical problems to be solved by the invention are to provide a package solution, while solving three addressed above
The a series of problem actually mutually involved together of aspect, also, the solution is used more logical applied to cholera diagnosis
Road micro-fluidic chip field, forms a kind of new cholera diagnosis and uses multichannel micro-fluidic device.
The present invention solves the technical problem by following scheme, and the device that the program is provided is that a kind of substrate of its chip is dredged by force
The cholera diagnosis multichannel micro-fluidic device of water, the structure of the device includes multichannel micro-fluidic chip, the micro-fluidic chip
Structure includes the substrate and cover plate of installing bonded to each other together, and the substrate and cover plate are plate object or tablet, the substrate
That face towards the cover plate contain via mould pressing process or etching technics formation channel structure, it is bonded to each other to be installed together
The substrate and the cover plate be built into the micro-fluidic chip containing pipeline configuration jointly, the locations of structures of the pipeline is located at the substrate
The juncture area bonded to each other with the cover plate, the two ends of the pipeline are connected with the sample introduction end of the micro-fluidic chip and terminal respectively,
The sample introduction end is the injection end of the micro-fluidic chip sample solution, and the terminal is its chip when the actual sample introduction of the micro-fluidic chip is tested
The terminal of interior sample solution flowing, the terminal is located remotely from each other with the sample introduction end, and the distance between the terminal and the sample introduction end are between 3
Centimetre and 10 centimetres between, working electrode is sequentially equiped with diverse location in the pipeline and to electrode and reference electrode,
The working electrode is quick by conductive electrode and the gold size for having embedded cholera specific antibody being attached on the conductive electrode
Feel film to constitute, the construction of the pipeline is in parallel construction, and the pipeline in parallel construction is made up of four lateral parallel connections, institute
The quantity for stating working electrode is four, and the installation position of four working electrodes is located in four laterals respectively, and,
Specific antigen in the sensitive membrane structure of its top layer gold size of four working electrodes can be specifically bound to cholera antibody respectively
Four kinds of cholera antigenic substances, four kinds of antigenic substances are cholera TP0821 antigens and cholera TP0319 antigens and suddenly respectively
Random TP0624 antigens and cholera O139 antigens, its material of the working electrode are gold material or thermal decomposition conducting polymer
Sheet or thread is presented in material, its pattern of the working electrode, and emphasis is that its material of the substrate is dimethyl silicone polymer material,
Its surface of the substrate is the surface of primary form, the surface of the primary form its be intended to refer to not repair by any surface chemistry
The surface of the primary form of decorations or the material of any surface chemical modification, the structure of the device also includes hinge-type fixture, the conjunction
Page fixture its appearance profile likeness in form hinge, the hinge-type fixture is by two hinges being mutually hinged and through this two
One fastening screw of hinge and one match and tight manually with being used for of connecting together of the fastening screw with the fastening screw
Gu and the nut of hand break loose is constituted, two hinges of the hinge-type fixture are respectively mutually drawn close with its tip and clamp the micro-fluidic core
Piece, the tip is positioned in the locations of structures of the neighbouring terminal of the micro-fluidic chip, a page at least in
Fixation is attached on piece and is equiped with miniature ultrasonic transducer units, and, higher-order of oscillation electric signal transmission cable, the higher-order of oscillation telecommunication
One end of number transmission cable links together with the miniature ultrasonic transducer units；The hinge-type fixture facilitates the device there is provided one
The function of disassembling；Its major function of the miniature ultrasonic transducer units is, in the actual sample introduction test of micro-fluidic chip, to be sent out using it
The ultrasonic wave penetrated reduces the interfacial tension between sample solution and the inwall of its inner passage of micro-fluidic chip, can phase
Hold, also, using the distance between the sample introduction end and the terminal and miniature ultrasonic transducer units installation position difference with
And its difference on the ultrasonic intensity experienced, its interfacial tension of sample introduction end described in induced synthesis and its interface of the terminal
Interfacial tension difference between difference between power, the micro-fluidic chip two ends can between the two ends of the micro-fluidic chip shape
Into pressure gap, the pressure gap can drive sample solution to the end flow；Its function of the miniature ultrasonic transducer units is also wrapped
Include the ultrasonic wave launched with it and check in sample contained its table in its inner passage of the micro-fluidic chip of large biological molecule
Absorption on face, and then check swallow up effect of its body phase of the substrate of the dimethyl silicone polymer material to the large biological molecule；It is soft
The substrate of the dimethyl silicone polymer material its function that is soft and having elasticity is included with its property to the strong absorption of ultrasonic wave, to super
Sound wave is absorbed strongly, and is thereby realized within micro-fluidic chip terminal to the limited short distance between the sample introduction end
The rapid decrement of ultrasonic intensity；And, Micropump, the Micropump is connected with the sample introduction end；The function of the Micropump is, in the sample
Interfacial tension between solution and the inwall of its inner passage of micro-fluidic chip is reduced by the ul-trasonic irradiation, alternate compatibility
Under increased precondition, the interface come with the mechanicalness pumping strength of the Micropump between the two ends of ultrasonic wave induction
Its driving force brought of power difference is supported mutually, adjusts mutually, is mutually coupled, and one is pooled in the way of Collaboration
Driving test liquid flows to the strength of the terminal direction flowing；Described its thickness of cover plate and substrate is between 1.0 millimeters and 5.0 millis
Between rice；And, ultrasonic wave damper, its profile of ultrasonic wave damper is in block, tabular or bar-shaped；The ultrasonic wave is cut down
Its material of device is microcellular rubber, sponge rubber or microporous polyurethane elastomer；And, elastic clip, the elastic force accompanies two clampings
Arm, the medial surface of each clamping limb is relative with the medial surface of another clamping limb, and the medial surface of one of clamping limb is close to this
The sample introduction end of micro-fluidic chip, the medial surface of another clamping limb is close to the ultrasonic wave damper, and the elastic clip is with its chucking power
Amount by the sample introduction end of the micro-fluidic chip and the ultrasonic wave damper it is bonded to each other clip together, the ultrasonic wave damper is with its material
The strong consumption to ultrasonic wave that matter is brought, is further supported in the micro-fluidic chip terminal between the sample introduction end
The rapid decrement of ultrasonic intensity is realized within limited short distance, and thereby further expand described its interfacial tension of sample introduction end with
Difference between its interfacial tension of the terminal.
Described miniature ultrasonic transducer units can certainly be all installed in two hinges；But, only installing one is micro-
Type ultrasonic transducer is dealt with enough to be used.
The word of hinge one art-recognized meanings of itself are known.
The word of Micropump one art-recognized meanings of itself are known for the professional in micro-fluidic chip field.
The Micropump such as miniature piezoelectric pump, miniature peristaltic pump or miniature air driven pump.
The Micropump both can be the Micropump of external form；The Micropump can also be done into or say inside the embedded micro-fluidic chip
The Micropump of the built in version of its sample introduction end structure position or the sample introduction end Near-neighbor Structure position.
The gold size sensitive membrane is to be sufficiently mixed chitosan gold size solution and cholera specific antigen solution uniformly, uses point sample instrument
Point sample is coated on specified structure position, and forms its drying and forming-film.Cholera specific antigen in the gold size sensitive membrane is equal
The cholera antigen marked for horseradish peroxidase or glucose oxidase, the gold size sensitive membrane has been included as fixing above-mentioned each
Cholera specific antigen and introduce complementary medium therein, the complementary medium such as chitosan, cellulose acetate, gelatin
Therein a kind of or their mixture.
The pipeline in the microfluidic chip structure includes the lateral, and its internal diameter size may each be arbitrarily selected
Size, still, for less with the consideration in terms of prepare liquid sample and reduction reagent loss, the pipeline includes described as far as possible
Lateral preferably selects the passage of capillary level, and the passage of the capillary level implies that its internal diameter and the capillary on ordinary meaning
The suitable passage of the internal diameter of pipe.The shape of cross section of its inner passage of capillary can be arbitrary shape, the cross section
Shape is for example circular, oval, square, rectangle, bar shaped, naturally it is also possible to be arbitrarily exist bending it is linear, also,
The interior shape of the capillary is with the extension of pipeline, and the shape of cross section of different parts can also allow to be different shapes.
Only for the word of capillary one, its art-recognized meanings is known.
What is be related in structure is the electrode of microsize to electrode and reference electrode, and its electrode shape may each be any choosing
Fixed shape, the arbitrarily selected shape such as square piece shape, rectangular patch, strip or round sheet etc..It is described to electricity
The art-recognized meanings of pole and the reference electrode vocabulary of itself are known.
It is public only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field
Know.
Various sizes, variously-shaped ultrasonic transducer are commercially available；Commercially available miniature ultrasonic transducer units its sizes can be with
It is small to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface itself and
Speech, is known general technology for the professional in ultrasonic technology field.This case is not to this expansion superfluous words.
Only with regard to naked PDMS substrates itself micro-channel molding or lithographic technique for, be open-and-shut known technology.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the micro fluidic device can also include higher-order of oscillation electric signal generator；The higher-order of oscillation electric signal transmission electricity
Its other end of cable can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, for the professional in ultrasonic technology field, be
It is simple and known；The higher-order of oscillation electric signal generator can be customized to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units be between 5 milliwatts and 9000 milliwatts it
Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with
Between 12MHz.
This case device can further include some annexes, the annex such as multiple tracks electrochemical workstation etc., institute certainly
The art-recognized meanings for stating multiple tracks electrochemical workstation are known.Each working electrode for being related in this case microfluidic chip structure and
, can be respectively via corresponding special the corresponding interface got lines crossed with the multiple tracks electrochemical workstation to electrode and reference electrode etc.
Coupled.It is described that special to get lines crossed be for each the corresponding interface of each electrode and the multiple tracks electrochemical workstation is carried out into phase
The private cable being mutually coupled with.The micro-fluidic chip in this case device, its structure can also include micro-valve, the number of the micro-valve
Amount is not limited, according to actual needs, and the micro-valve can be installed in any required position installed in the microfluidic chip structure；
The word of micro-valve one is for the professional of micro fluidic chip technical field, and the art-recognized meanings of itself are known；The micro-valve
The manufacturing technology of itself and the use of technology is also known；The component that the micro-valve is not required.
The diameter of the working electrode can allow to be that any setting is easily installed the suitable diameter used, it is however recommended to
Or say preferred its scope of the diameter between 0.1 micron to 2000 microns；The length of the working electrode can permit
Permitted to be that any setting is easily installed the length used, it is however recommended to or to say preferred its scope of the length be at 1 micron
To between 15000 microns.
The gold size for being installed in the working electrode surface layer by spraying or point sample instrument point sample or the coating of other appropriate process is quick
Feel film, its thicknesses of layers can allow be any setting treat sample measuring liquid occur electrical signals response thickness, still, push away
Preferred thickness is between 10 nanometers and 200 nanometers in other words for the thickness recommended.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, for example：Polypropylene, glass
Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, such as do
Realized into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and in the extremely short distance to ultrasonic wave
Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip
It is used as the cover plate using dimethyl silicone polymer, is also that this case technical scheme is allowed.
Described its thickness of cover plate and substrate can allow be any setting be easy to assembling thickness, the thickness of recommendation or say preferably
Thickness be between 1.0 millimeters and 5.0 millimeters.Less thickness is conducive to saving material.
Described its size of ultrasonic wave damper is not limited, and described its length of ultrasonic wave damper, width, thickness may each be according to need
The arbitrary size to be set.
It is known in field of rubber technology only for the word of microcellular rubber one art-recognized meanings of itself.
The microcellular rubber is commercially available.
It is known in field of rubber technology only for the word of sponge rubber one art-recognized meanings of itself.
The sponge rubber is commercially available.
It is known in technical field of macromolecules only for the word of microporous polyurethane elastomer one art-recognized meanings of itself.
The polyurethane micropore elastomer material is commercially available.
The microcellular rubber preferred microporous silicon rubber or micropore fluorubber.
It is known in field of rubber technology only for the word of micropore silicon rubber one art-recognized meanings of itself.
The microporous silicon Rubber market is on sale.
It is known in field of rubber technology only for the word of micropore fluorubber one art-recognized meanings of itself.
The micropore fluorubber is commercially available.
The word of elastic clip one art-recognized meanings of itself are known.
The elastic clip is commercially available.
The application method of this case micro-fluidic chip：
This case is flowed with dual drive coupling operating mode driving liquid stream in the capillary channel of the four-way micro-fluidic chip,
Four kinds of cholera antibody are detected respectively using four-way electrochemical analytical instrument.
Corresponding four kinds of cholera antibody, four kinds of cholera corresponding with being detected with four kinds of cholera antibody are detected with four kinds of cholera antigens
Antigen, similarly, can cholera diagnosis；The principle of its foundation is, necessarily antigen, antibody and is deposited in cholera patient,
And mutual Reversible binding, reversible conjugate is formed, therefore, antibody and thereby cholera diagnosis are detected with antigen, and with antibody
Detecting antigen and cholera diagnosis are removed, diagnostic purpose can be reached.Certainly, its corresponding electrode sensitive decorative layer of different means
Technically and differ.
The specific detection of this case micro-fluidic chip is as follows using step：
1st, blood serum sample liquid is added in micro-pipe road, under dual drive coupling operating mode driving, various cholera antibody point
The cholera specific antigen for the corresponding horseradish peroxidase-labeled that son is embedded by gold size sensitive membrane on electrode surface in each passage
Capture.
2nd, the cholera antibody formation immune complex in the cholera specific antigen and blood serum sample of horseradish peroxidase-labeled.
3rd, using multi-channel electrochemical analyzer, the electron mediators such as catechol are added, using the above-mentioned reaction of amperometric detection
Caused curent change, is derived from the species and content of various analytes.
4th, result is subjected to comprehensive analysis, comprehensive diagnos is carried out to cholera antibody.
It is an advantage of the invention that its close position positions the hinge-type fixture in the terminal of the micro-fluidic chip, with
The miniature ultrasonic transducer units installed, the low-power launched using it, high-frequency band are attached in its hinge of the hinge-type fixture
Ultrasonic wave so that without surface chemical modification or surface chemical modification strong hydrophobic micro-fluidic chip internal pipeline its
Compatibility between tube wall and the test object aqueous solution is significantly increased, and this passes through possible there is provided a reality for test liquid stream
Property；Meanwhile, using its strong absorbability to ultrasonic wave of dimethyl silicone polymer substrate, in shorter distance, also
Be, from the terminal to the very short distance of only several centimeters yardsticks the sample introduction end in, reach the quick of ultrasonic intensity
Successively decrease, interfacial tension between the difference of the interfacial tension, the two ends is thereby caused at the two ends of the micro-fluidic chip
Difference can cause a kind of driving force, this kind because caused by interfacial tension difference its function of driving force be to drive test liquid stream to exist
Originally flowed in strong hydrophobic capillary channel to the terminal direction；And the simultaneous Micropump in structure, its function
It is that the interfacial tension between the inwall of the sample solution and its inner passage of micro-fluidic chip is dropped by the ul-trasonic irradiation
Under low, the alternate increased precondition of compatibility, described two induced with the ultrasonic wave are come with the mechanicalness pumping strength of the Micropump
Interfacial tension difference its driving force brought between end is supported mutually, adjusts mutually, is mutually coupled, with Collaboration
Mode pools the strength that one driving test liquid flows to the terminal direction flowing；The presence of the Micropump so that the miniature ultrasonic
Its ultrasonic wave emissive porwer of wave transducer can be allowed to appropriate reduction, and this in detection object for containing ultrasonic wave sensitive composition
Situation is particularly suitable for；And by the presence of the ultrasonic transducer and its radiated ultrasonic wave, it is possible to increase alternate compatibility, drop
Low interfacial tension, and the two ends interfacial tension difference special driving force that it is brought is provided, then, in this case,
Its flow resistance in the micro-channel of test liquid stream is greatly reduced, and correspondingly, its running resistance of the Micropump is greatly reduced, so,
The Micropump just can be to carry out the pumping work for the sample solution than relatively low pumping pressure, due to mechanical pumping
Pressure is greatly reduced, therefore, in such a situation, be just not susceptible to because sample introduction terminal tool pumping pressure it is excessive caused by
Between the micro-channel bubbling of the sample introduction end and its near zone, expansion, deformation, distortion and the region substrate and cover plate
The problems such as stripping etc.；This case dual drive couples the scheme of running and adds the manipulation acted for the sample fluid flow
Property, it can allow for coming using the multiple indexs of ultrasonic intensity, ultrasonic frequency, Micropump pump power, Micropump pumping pressure etc.
Flow rate, flowing action for the flowing include flow forward or suspend flowing or accelerate flowing etc. flowing action progress many
Parameter is accurately manipulated；By the scheme of this case dual drive coupling running, entirely without must be to the base of the dimethyl silicone polymer material
Any surface chemical modification of its micro-channel of piece etc. relevant surfaces progress or surface chemical modification, have altogether dispensed with the surface chemistry
Modification or the laborious procedures of surface chemical modification；On the other hand, the ultrasonic wave of the low-power, high-frequency band, additionally it is possible to containment examination
The absorption of large biological molecule in sample on literalness naked its inner surface of pipeline of dimethyl silicone polymer substrate, and then contain
The swallow up effect of its body phase of dimethyl silicone polymer substrate to the large biological molecule；The antigen, antibody and antigen are with resisting
The Reversible binding thing of body is all the type for belonging to described large biological molecule certainly；Due to described suction-operated and described gulp down
Do not act on and effectively being contained, therefore, dependence test result will be better able to objectively reflect actual conditions；The low-power, height
The effect of frequent section ultrasonic wave, also include facilitating the Reversible binding between antigen, antibody react certainly quickly reaches, and this causes
Dependence test operation can be with than the completion of faster speed.
As described above, the presence of the Micropump so that its ultrasonic wave emissive porwer of the miniature ultrasonic transducer units can be allowed to fit
Degree reduction, then, the feature helps to protect its sensitive coating of the working electrode, is allowed to from ultrasound injury.
Based on this case scheme, repaiied completely without the surface chemistry carried out for the dimethyl silicone polymer substrate its relevant surfaces
Decorations or surface chemical modification operation, therefore, this surface chemical modification layer or surface chemical modification layer not need to exist,
So, its body phase interior polymer molecule of the dimethyl silicone polymer substrate is constantly diffused to the surface, migrated caused by it automatically
Damaging influence to surface chemical modification layer or surface chemical modification layer is also just not present.
This case its installation position is located at the ultrasonic wave damper at the sample introduction end, from its terminal of this case micro-fluidic chip to entering
Said on the sport technique segment that ultrasonic intensity rapid decrement is realized in the such short distance in sample end, the ultrasonic wave damper its function as
The size expansion device of micro-fluidic chip, in other words, the ultrasonic wave damper its function as significantly extend it is micro-fluidic
Its terminal of chip the distance between to sample introduction end, be the equal of from length of several centimetres of the extension of script to more than ten centimetres or
Twenties centimetres of length or longer length, so so that described terminal is strong to the ultrasonic wave between sample introduction end in short distance
The sport technique segment of degree rapid decrement can relatively easily be reached, also, the size expansion device namely the ultrasonic wave damper its
Installation and removal are all very convenient, it is important that it can also be reused.
Its presence of the ultrasonic wave damper in this case device, further reduce to its pumping pressure of Micropump and pump power according to
Rely；Correspondingly, its running resistance of the Micropump is further greatly reduced, so, and the Micropump just can be with lower pumping
Pressure carries out the pumping work for the sample solution, because mechanical pumping pressure is further greatly reduced,
Therefore, in such a situation, just be less susceptible to occur because sample introduction terminal tool pumping pressure it is excessive caused by the sample introduction end and
The problems such as stripping between the micro-channel bubbling of its near zone, expansion, deformation, distortion and the region substrate and cover plate etc.；
The scheme of this case dual drive coupling running is further added for the test liquid due to its presence of the ultrasonic wave damper
The handling of flowing action is flowed, wherein, the factor that can adjust includes ultrasonic intensity, ultrasonic frequency, Micropump pump power, micro-
Pump pumps pressure, and, the selection and use of ultrasonic wave damper material, the selection of ultrasonic wave damper block size and use,
The selection of the size of its corresponding ultrasonic wave abatement ability of ultrasonic wave damper and use etc., this kind of many adjustable factor it is same
When exist, can more operation indexs, further, more it is multifactor adjustably for the flowing flow rate, stream
Action includes flow forward or pause flowing or accelerates flowing etc. flowing action to carry out the accurate manipulation under more parameters.
The technical scheme of this case has dissolved its application related one to dimethyl silicone polymer substrate addressed above totally
Row technical barrier.Based on this case scheme, the polydimethyl siloxane material of this kind very cheap and easily processing and fabricating just has can
Can in the micro-fluidic chip its prepare, production, using etc. field play bigger effect.
The miniature ultrasonic transducer units have been installed in fixation in the hinge-type fixture its hinge in this case structure, and the structure is provided
One facilitates the function that the device is disassembled, in this way, the hinge-type fixture is together with appended miniature ultrasonic transducer units in its hinge
Just easily it can be mutually disengaged with the micro-fluidic chip, then, the component that the part can freely depart from just can circulate benignly ground
Reuse many times；The architectural feature is conducive to saving the use cost of the device.
Brief description of the drawings
Fig. 1 is its rough outside side view of this case micro flow control chip device.
In figure, 1 is hinge-type fixture, and 2 indicate linkwork positions, and 3 be fastening screw, and 4 be higher-order of oscillation telecommunication
Number transmission cable, 5 be miniature ultrasonic transducer units, and 6,11 be two different hinges of locations of structures respectively, and 7 be cover plate, 8
It is substrate, 9 be sample introduction end, and 10 be terminal, and 12 be nut, and 13 be infusion tube, and 14 be Micropump；The conjunction in legend
Page clamp structure is only the legend structure of signal, and actual its structure of the hinge-type fixture is not limited to the legend hinge-type fixture knot
Structure；Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its test liquid stream
Flow direction.The ultrasonic wave damper is depicted without in legend；Also the elastic clip is depicted without in legend.
Embodiment
In this case that Fig. 1 is shown embodiment, the structure of the device includes multichannel micro-fluidic chip, the micro-fluidic core
The structure of piece includes the substrate 8 and cover plate 7 of installing bonded to each other together, and the substrate 8 and cover plate 7 are plate object or piece
Contain the channel structure via mould pressing process or etching technics formation, phase in shape thing, that face towards the cover plate 7 of the substrate 8
The substrate 8 being mutually jointly mounted to together has been built into the micro-fluidic chip containing pipeline configuration jointly with the cover plate 7, the pipeline
Locations of structures be located at the substrate 8 and the cover plate 7 juncture area bonded to each other, the two ends of the pipeline respectively with the micro-fluidic core
The sample introduction end 9 of piece and terminal 10 are connected, and the sample introduction end 9 is the injection end of the micro-fluidic chip sample solution, the terminal 10
It is the terminal of sample solution flowing in its chip when the actual sample introduction of the micro-fluidic chip is tested, the terminal 10 and the phase of sample introduction end 9
Mutually remote, the distance between the terminal 10 and the sample introduction end 9 are between 3 centimetres and 10 centimetres, the different positions in the pipeline
Put and be sequentially equiped with working electrode and to electrode and reference electrode, the working electrode is by conductive electrode and is attached to
The gold size sensitive membrane for having embedded cholera specific antibody on the conductive electrode is constituted, and the construction of the pipeline is in parallel construction, institute
State and be made up of in the pipeline of parallel construction four lateral parallel connections, the quantity of the working electrode is four, this four work electricity
The installation position of pole is located in four laterals respectively, and, the sensitive membrane structure of its top layer gold size of four working electrodes
In specific antigen be the four kinds of cholera antigenic substances that can be specifically bound to cholera antibody respectively, four kinds of antigenic substances difference
It is cholera TP0821 antigens and cholera TP0319 antigens and cholera TP0624 antigens and cholera O139 antigens, institute
Stating working electrode, its material is gold material or thermally decomposes conducting polymer material, and sheet or silk is presented in its pattern of the working electrode
Shape, emphasis is, its material of the substrate 8 is dimethyl silicone polymer material, and its surface of substrate 8 is the surface of primary form,
The surface of the primary form its be intended to refer to the material not no by any surface chemical modification or any surface chemical modification
Primary form surface, the structure of the device also includes hinge-type fixture 1, and its appearance profile likeness in form of hinge-type fixture 1 is closed
Page, the hinge-type fixture 1 by two hinges 6,11 for being mutually hinged and through two hinges 6,11 one
Individual fastening screw 3 and one matched with the fastening screw 3 and fastened manually with being used for of connecting together of the fastening screw 3 and
The nut 12 of hand break loose is constituted, and the nut 12 is employed in this example is easier manually operated butterfly nut, butterfly
Nut is commercially available, and two hinges 6,11 of the hinge-type fixture 1 are respectively mutually drawn close with its tip and clamp the micro-fluidic core
Piece, the tip is positioned in the locations of structures of the neighbouring terminal 10 of the micro-fluidic chip, an institute at least in
State attaching fixation in hinge to be equiped with miniature ultrasonic transducer units, this example, the miniature ultrasonic transducer units 5 are to attach to be fixed on
In hinge 6, and, higher-order of oscillation electric signal transmission cable 4, one end of the higher-order of oscillation electric signal transmission cable 4 is micro- with this
Type ultrasonic transducer 5 links together；The hinge-type fixture 1 facilitates the function that the device is disassembled there is provided one；This is miniature
Its major function of ultrasonic transducer 5 is that in the test of micro-fluidic chip actual sample introduction, the ultrasonic wave launched using it is reduced
Interfacial tension between sample solution and the inwall of its inner passage of micro-fluidic chip, can be compatible, also, utilizes institute
State the distance between sample introduction end 9 and the terminal 10 and the installation position of miniature ultrasonic transducer units 5 difference and it is felt
The difference on ultrasonic intensity being subject to, its interfacial tension of sample introduction end 9 described in induced synthesis and described its interfacial tension of terminal 10
Between difference, interfacial tension difference between the micro-fluidic chip two ends 9,10 can the two ends 9 of the micro-fluidic chip,
Pressure gap is formed between 10, the pressure gap can drive sample solution to be flowed to the direction of terminal 10；The miniature ultrasonic
Transducer 5 its function also includes checking large biological molecule contained in sample with its ultrasonic wave launched that it is micro-fluidic at this
Absorption on its inner passage inner surface of chip, and then check the substrate 8 of the dimethyl silicone polymer material its body phase to the biology
The effect of swallowing up of macromolecular；It is soft and have the substrate 8 of the dimethyl silicone polymer material of elasticity its function and include with it to ultrasound
The property that ripple absorbs strongly, is absorbed strongly to ultrasonic wave, and thereby in the micro-fluidic chip, the terminal 10 arrives the sample introduction end
The rapid decrement of ultrasonic intensity is realized within limited short distance between 9；And, Micropump 14, the Micropump 14 enters with this
Sample end 9 is connected；The function of the Micropump 14 is, between the inwall of the sample solution and its inner passage of micro-fluidic chip
Interfacial tension is reduced by the ul-trasonic irradiation, under the increased precondition of alternate compatibility, with the mechanicalness pump of the Micropump 14
Interfacial tension difference its driving force brought between the two ends 9,10 that strength induces come the ultrasonic wave is sent to prop up mutually
Hold, adjust mutually, be mutually coupled, one driving test liquid is pooled in the way of Collaboration and flows to the direction of terminal 10
The strength of flowing；Its thickness of the cover plate 7 and substrate 8 is between 1.0 millimeters and 5.0 millimeters；And, ultrasonic wave disappears
Subtract device, its profile of ultrasonic wave damper is in block, tabular or bar-shaped；Its material of the ultrasonic wave damper is microcellular rubber, sea
Continuous rubber or microporous polyurethane elastomer；And, elastic clip, the elastic force accompanies two clamping limbs, the medial surface of each clamping limb
Relative with the medial surface of another clamping limb, the medial surface of one of clamping limb is close to the sample introduction end 9 of the micro-fluidic chip,
The medial surface of another clamping limb is close to the ultrasonic wave damper, the elastic clip entering the micro-fluidic chip with its gripping strength
Sample end 9 and the ultrasonic wave damper are clipped together bonded to each otherly, the ultrasonic wave damper with its material brought to ultrasonic wave
Strong consumption, further support the limited short distance arrived in the micro-fluidic chip terminal 10 between the sample introduction end 9
Within realize the rapid decrement of ultrasonic intensity, and thereby further expand described its interfacial tension of sample introduction end 9 and the terminal
Difference between 10 its interfacial tension.
The ultrasonic wave damper is depicted without in legend；Also the elastic clip is depicted without in legend.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by two ends 9,10 pressure differentials drive, its
The flow direction of test liquid stream.
Fig. 1 is depicted without the associate members such as the higher-order of oscillation electric signal generator.
The Micropump 14 can be customized to specialized factory.
Its connected mode with various micro-fluidic chip of various Micropump is known.
Described its main body of hinge-type fixture can simply be changed a social system using commercially available hinge；It can also be customized to hardware processing factory.
Involved miniature ultrasonic transducer units 5 are commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal transmission cable 4 is commercially available；Can also be to ultrasonic transducer producer or cable special manufacturer
Family's customization.
Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also be customized to relevant manufacturers.
Its inner passage of the micro-fluidic chip involved by this case is the pipeline of hydrophobic capillary form.
The Micropump both can be the Micropump of external form；The Micropump can also be done into or say inside the embedded micro-fluidic chip
The Micropump of the built in version of its sample introduction end structure position or the sample introduction end Near-neighbor Structure position, its basic structure key element and external form
Micropump it is identical.Micropump in legend is the Micropump of external form.
Each working electrode in this example structure and electrode and reference electrode via each special cable or can be said respectively
Get lines crossed respectively with the corresponding cable interface of the multiple tracks electrochemical workstation as annex or saying interface connection of getting lines crossed.
This case device is that a variety of corresponding cholera antibody are detected with a variety of cholera antigens, thereby cholera diagnosis.
Claims (10)
1. a kind of strong hydrophobic cholera diagnosis multichannel micro-fluidic device of substrate of its chip, the structure of the device includes multichannel
Micro-fluidic chip, the structure of the micro-fluidic chip includes the substrate and cover plate of installing bonded to each other together, the substrate and cover plate
It is plate object or tablet, the groove via mould pressing process or etching technics formation is contained in that face towards the cover plate of the substrate
Road structure, substrate bonded to each other being installed together has been built into the micro-fluidic chip containing pipeline configuration jointly with the cover plate,
The locations of structures of the pipeline is located at the substrate and cover plate juncture area bonded to each other, and the two ends of the pipeline are micro-fluidic with this respectively
The sample introduction end of chip and terminal connection, the sample introduction end is the injection end of the micro-fluidic chip sample solution, and the terminal is the miniflow
The terminal of sample solution flowing in its chip when the actual sample introduction of chip is tested is controlled, the terminal is located remotely from each other with the sample introduction end, the terminal
With the distance between the sample introduction end between 3 centimetres and 10 centimetres, work is sequentially equiped with diverse location in the pipeline
Electrode and to electrode and reference electrode, the working electrode is by conductive electrode and the bag being attached on the conductive electrode
The gold size sensitive membrane for having buried cholera specific antibody is constituted, and the construction of the pipeline is in parallel construction, the pipeline in parallel construction
It is made up of four lateral parallel connections, the quantity of the working electrode is four, the installation position difference position of four working electrodes
In in four laterals, and, the specific antigen difference in the sensitive membrane structure of its top layer gold size of four working electrodes
It is the four kinds of cholera antigenic substances that can be specifically bound to cholera antibody, four kinds of antigenic substances are cholera TP0821 antigens respectively
And cholera TP0319 antigens and cholera TP0624 antigens and cholera O139 antigens, its material of the working electrode is
Sheet or thread is presented in gold material or thermal decomposition conducting polymer material, its pattern of the working electrode, it is characterised in that should
Its material of substrate is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the surface of the primary form its
It is intended to refer to not by any surface chemical modification or the surface of the primary form of the material of any surface chemical modification, should
The structure of device also includes hinge-type fixture, hinge-type fixture its appearance profile likeness in form hinge, and the hinge-type fixture is by mutually cutting with scissors
Two hinges being connected together and matched through a fastening screw of two hinges and one with the fastening screw and with
The nut for being used for fastening and hand break loose manually that the fastening screw connects together is constituted, and two hinges of the hinge-type fixture are each
Mutually drawn close with its tip and clamp the micro-fluidic chip, the tip is positioned at the knot of the neighbouring terminal of the micro-fluidic chip
Fixation, which is attached, on structure position, in a hinge at least in is equiped with miniature ultrasonic transducer units, and, high frequency vibrating
Electric signal transmission cable is swung, one end and the miniature ultrasonic transducer units of the higher-order of oscillation electric signal transmission cable link together；
The hinge-type fixture facilitates the function that the device is disassembled there is provided one；Its major function of the miniature ultrasonic transducer units is in miniflow
When controlling the actual sample introduction of chip and testing, the ultrasonic wave launched using it reduces sample solution and its inner passage of the micro-fluidic chip
Inwall between interfacial tension, can be compatible, also, utilize the sample introduction end and the terminal and the miniature ultrasonic
Difference in the distance between wave transducer installation position difference and its ultrasonic intensity experienced, enters described in induced synthesis
Interfacial tension between difference between its interfacial tension of sample end and the terminal its interfacial tension, the micro-fluidic chip two ends is poor
Different to form pressure gap between the two ends of the micro-fluidic chip, the pressure gap can drive sample solution to the terminal stream
It is dynamic；Its function of the miniature ultrasonic transducer units also includes checking large biological molecule contained in sample with its ultrasonic wave launched
Its absorption on the micro-fluidic chip its inner passage inner surface, and then check the substrate of the dimethyl silicone polymer material its body
With respect to the effect of swallowing up of the large biological molecule；It is soft and have the substrate of the dimethyl silicone polymer material of elasticity its function include with
Its property to the strong absorption of ultrasonic wave, is absorbed strongly to ultrasonic wave, and thereby in the micro-fluidic chip, the terminal is entered to this
The rapid decrement of ultrasonic intensity is realized within limited short distance between sample end；And, Micropump, the Micropump and the sample introduction end
Connection；The function of the Micropump is, the interfacial tension between the inwall of the sample solution and its inner passage of micro-fluidic chip by
The ul-trasonic irradiation and reduce, under the increased precondition of alternate compatibility, come super with this with the mechanicalness pumping strength of the Micropump
Interfacial tension difference its driving force brought between the two ends of sound wave induction supports mutually, adjustment, mutual coupling mutually
Close, the strength that one driving test liquid flows to the terminal direction flowing is pooled in the way of Collaboration；The cover plate and base
Its thickness of piece is between 1.0 millimeters and 5.0 millimeters；And, ultrasonic wave damper, its profile of the ultrasonic wave damper is in
Block, tabular or bar-shaped；Its material of the ultrasonic wave damper is microcellular rubber, sponge rubber or microporous polyurethane elastomer；With
And, elastic clip, the elastic force accompanies two clamping limbs, and the medial surface of each clamping limb is relative with the medial surface of another clamping limb,
The medial surface of one of clamping limb is close to the sample introduction end of the micro-fluidic chip, and the medial surface of another clamping limb is close to this and surpassed
Sound wave damper, the elastic clip is bonded to each other by the sample introduction end of the micro-fluidic chip and the ultrasonic wave damper with its gripping strength
Clip together, the strong consumption to ultrasonic wave that the ultrasonic wave damper is brought with its material, further support micro- at this
The fluidic chip terminal is thereby entered to realizing the rapid decrement of ultrasonic intensity within the limited short distance between the sample introduction end
One step expands the difference between described its interfacial tension of sample introduction end and the terminal its interfacial tension.
2. multichannel micro-fluidic device is used in a kind of strong hydrophobic cholera diagnosis of the substrate of its chip according to claim 1, its
It is characterised by, it is capillary channel that the pipeline, which includes the lateral,.
3. multichannel micro-fluidic device is used in a kind of strong hydrophobic cholera diagnosis of the substrate of its chip according to claim 1, its
Be characterised by, the thermal decomposition conducting polymer be by polyimides or polyacrylonitrile be heat-treated through anoxybiotic after the electric conductivity material that is formed
Material.
4. multichannel micro-fluidic device is used in a kind of strong hydrophobic cholera diagnosis of the substrate of its chip according to claim 1, its
Be characterised by, the width or diameter of the working electrode between 0.1 micron to 2000 microns, and, work electricity
The length of pole is between 1 micron to 15000 microns.
5. multichannel micro-fluidic device is used in a kind of strong hydrophobic cholera diagnosis of the substrate of its chip according to claim 1, its
It is characterised by, the thickness of the gold size sensitive membrane is between 10 nanometers and 200 nanometers.
6. multichannel micro-fluidic device is used in a kind of strong hydrophobic cholera diagnosis of the substrate of its chip according to claim 1, its
It is characterised by, its material of the cover plate in structure is dimethyl silicone polymer material.
7. multichannel micro-fluidic device is used in a kind of strong hydrophobic cholera diagnosis of the substrate of its chip according to claim 1, its
It is characterised by, the Micropump is miniature piezoelectric pump, miniature peristaltic pump or miniature air driven pump.
8. multichannel micro-fluidic device is used in a kind of strong hydrophobic cholera diagnosis of the substrate of its chip according to claim 1, its
It is characterised by, the microcellular rubber is micropore silicon rubber or micropore fluorubber.
9. multichannel micro-fluidic device is used in a kind of strong hydrophobic cholera diagnosis of the substrate of its chip according to claim 1, its
It is characterised by, the structure of the micro fluidic device also includes higher-order of oscillation electric signal generator, the higher-order of oscillation electric signal transmission electricity
Its other end of cable is connected with the higher-order of oscillation electric signal generator.
10. multichannel micro-fluidic device is used in a kind of strong hydrophobic cholera diagnosis of the substrate of its chip according to claim 1, its
It is characterised by, its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 5 milliwatts and 9000 milliwatts, and this is micro-
The frequency of its ultrasonic wave operationally launched of type ultrasonic transducer is between 100KHz and 12MHz.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101620227A (en) * | 2009-07-12 | 2010-01-06 | 宁波大学 | Multi-channel chip for cholera diagnosis based on structural conductive macromolecular material technology |
| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
| CN102645429A (en) * | 2012-04-23 | 2012-08-22 | 宁波大学 | Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device |
| CN102645428A (en) * | 2012-03-08 | 2012-08-22 | 宁波大学 | Electrochemiluminescence analysis and test system with easy-to-dismount additional functional mechanism |
| CN107138192A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | A kind of easily-disassembled cholera diagnosis micro fluidic device of dual drive CGCM |
-
2016
- 2016-03-15 CN CN201610176666.1A patent/CN107185603A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101620227A (en) * | 2009-07-12 | 2010-01-06 | 宁波大学 | Multi-channel chip for cholera diagnosis based on structural conductive macromolecular material technology |
| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
| CN102645428A (en) * | 2012-03-08 | 2012-08-22 | 宁波大学 | Electrochemiluminescence analysis and test system with easy-to-dismount additional functional mechanism |
| CN102645429A (en) * | 2012-04-23 | 2012-08-22 | 宁波大学 | Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device |
| CN107138192A (en) * | 2016-03-01 | 2017-09-08 | 李榕生 | A kind of easily-disassembled cholera diagnosis micro fluidic device of dual drive CGCM |
Non-Patent Citations (1)
| Title |
|---|
| JAMES FRIEND等: "Microscale acoustofluidics: Microfluidics driven via acoustics and ultrasonics", 《REVIEWS OF MODERN PHYSICS》 * |
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Application publication date: 20170922 |