CN107167505A - The hypotype swine flu multi-channel testing device of dual drive coupling containing hydrophobic substrate - Google Patents

The hypotype swine flu multi-channel testing device of dual drive coupling containing hydrophobic substrate Download PDF

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CN107167505A
CN107167505A CN201610167662.7A CN201610167662A CN107167505A CN 107167505 A CN107167505 A CN 107167505A CN 201610167662 A CN201610167662 A CN 201610167662A CN 107167505 A CN107167505 A CN 107167505A
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micro
substrate
fluidic chip
ultrasonic wave
sample introduction
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李榕生
葛宇杰
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/11—Orthomyxoviridae, e.g. influenza virus

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  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Molecular Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Electrochemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The present invention relates to a kind of hypotype swine flu multi-channel testing device of the dual drive coupling containing hydrophobic substrate, belong to analysis testing field.The serial problem of substrate physical presence for making hypotype swine flu diagnosis micro-fluidic chip using dimethyl silicone polymer i.e. PDMS that is cheap and easily processing；This case is directed to the serial problem.This case main points are, the selected PDMS with ecosystem surface of substrate, and the chain ring type magnetic holding device for being equiped with miniature ultrasonic transducer units is socketed is positioned at the test liquid stream terminal of the micro-fluidic chip its neighbor positions, simultaneously, ultrasonic wave damper is installed at the sample introduction end of the micro-fluidic chip, reach ultrasonic intensity rapid decrement in short distance, so as to form interfacial tension difference at the two ends of the chip, difference offer is a kind of to drive the strength that test liquid stream flows along hydrophobic capillary channel to the terminal direction, the strength while with Micropump its mechanicalness pumping strength Collaboration for being included in structure.

Description

The hypotype swine flu multi-channel testing device of dual drive coupling containing hydrophobic substrate
Technical field
The present invention relates to a kind of hypotype swine flu multi-channel testing device of the dual drive coupling containing hydrophobic substrate, the device is to be based on Antigen/antibody specific reaction diagnoses the special purpose device of hypotype swine flu antigen, belongs to analysis testing field.
Background technology
Multichannel micro-fluidic hypotype swine flu diagnostic techniques background, may refer to the patent of invention such as CN 201110311127.1 Shen Please case.
Only for the microflow control technique overall general picture of itself, it may refer to famous micro-fluidic expert Mr. Lin Ping Cheng and go out not long ago Monograph " diagram Microfluid based Lab on a chip ", the monograph is published via Science Press, and the monograph is for microflow control technique Past, now, and, in terms of vision of the future etc., suffer from detail, be deep into long discussion of detail.
So, the Important Problems of this case that to have a talk below concern.
The basic framework of micro-fluidic chip, including it is etched with the substrate of small fluid course and the cover plate fit together therewith, Small fluid course on the substrate, assembling upper cover plate before, it is apparent on see to be exactly some micro-channels, wait until thereon After covering cover plate, just real closure forms the small fluid course, and the conduit inner surface of the micro-channel is together with surround this The part cover plate of micro-channel constitutes described small fluid course together；So, it is clear that this after assembling is completed is small Fluid course, the major part of its inner surface area is the inner surface area of that micro-channel, in other words, in the micro-channel The state or property on surface substantially determine the integrality or property of the small fluid course；Therefore say, this is built in base The inner surface state or inner surface property of micro-channel on piece are key factors；In principle, it is any to keep or protecting substantially The material of its solid forms is held, can be used to make substrate and cover plate, such as, the material that can act as substrate and cover plate can be with It is monocrystalline silicon piece, quartz plate, sheet glass, high polymer such as dimethyl silicone polymer, polymethyl methacrylate, makrolon etc. Deng；Certainly, the selection of substrate and the selection of cover plate be able to can also be differed with identical；From material consumption, manufacture difficulty and From the point of view of in terms of application popularization prospect etc., between these materials exist not small difference, the especially selection of that substrate, influence compared with Greatly.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, so Substrate on make that micro-channel is extremely simple, and the lower cost for material makes substrate with the polydimethyl siloxane material, Micro-channel is being suppressed or etched thereon, and is being engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes, It is a kind of more satisfactory selection seemingly；Certainly, patch material can also select to use cheap polydimethyl siloxane material： So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, makes extremely simple, seems and should also be as It is extremely easy to popularize, promotes.
But, thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that the letter PDMS that abridges is referred to, itself are a kind of strong Hydrophobic material, micro-channel is built on this material, if without being operated for the modified of the micro-channel surface, then, After overall assembling is completed, that is, cover after cover plate, because the micro-channel its inner surface in structure occupies most liquid circulation The inner surface in road, then, its strong hydrophobic property of the PDMS micro-channels inner surface, is deciding factor, it can cause class Be similar to the aqueous solution the fine liquid stream of polar liquid by becoming very difficult, its flow resistance is big, or even general Micropump is all It is difficult to promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar； Therefore, among prior art, modification is modified particular for the micro-channel inner surface on the PDMS material, is necessary Operation；So, this is pretty troublesome for the modified operation of PDMS micro-channel inner surfacesThat falls nor this problem, structure Perplex into serious technical, be another problem：PDMS polymer molecules tool inside its body phase of this PDMS material substrate There are PDMS polymer molecules inside the characteristic for diffusing to the surface, migrating automatically, this substrate body phase to diffuse to the surface, move automatically The characteristic of shifting, will cause the state after modification by its inner surface of that micro-channel of surface modifying and decorating can not maintain foot Enough long time, the holding time for micro-channel its inner surface state after that is surface-modified is substantially only sufficient to complete in laboratory The time of portion's test experiments needs；In other words, by surface modification or the PDMS micro-channel inner surfaces of surface modification, its The surface state that is formed can not be lasting after modification or after saying modification, but soon automatically tend to or say and become surface again and change Surface state before property, returns to the strong hydrophobic surface state of that script in the shorter time, then, just think, Such micro-fluidic chip can largely make, mass storage, be widely popularized, and answer is it is obvious that is, impossible. Micro-channel on this PDMS material, can not pump similar to the fine liquid stream of polar solvent of the aqueous solution if not doing surface modification Send and pass through, chip also cannot just be used；And if having done surface modification, the state after its modification can not be persistently kept again, also Being equally can not popularization and application.
So, how to accomplish that substrate can either be made using cheap PDMS material, and table in the micro-channel can be released Face decorating state can not persistently, chip can not largely make, largely lay in so that be widely popularized it is such a make this area it is numerous specially The puzzlement that industry personnel are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, above it is stated that, its surface is strongly hydrophobic, this strong hydrophobic Material surface and also another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and And, these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into gradually deep, directly It is trapped in heavy within the body phase of PDMS substrates, in fact, this process is partly also due to inside PDMS material body phase Polymer molecule, which has, to be diffused to the surface, caused by travel motion；Such case, can also be explained from another angle, i.e. Continuously from inside PDMS body phases to its diffusion into the surface, migration those polymer molecules, its result moved, be by Gradually those are involved within the body phase of PDMS substrates by the large biological molecule of adsorption, briefly, these are inhaled Attached large biological molecule is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up large biological molecule Phenomenon, the influence caused by it necessarily causes the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, be the problem of PDMS substrates, its not only adsorption large biological molecule, and swallow up large biological molecule, So, as the large biological molecule of experiment test object, its disappearance will not stop because the absorption of surface saturation, but, no It is disconnected adsorbed, also constantly swallowed up.
On PDMS substrates in related experiment test process its body phase constantly swallow up test associated biomolecule macromolecular phenomenon, separately It is to say that one kind, which is explained, there are substantial amounts of Minute pores in PDMS body phases, and associated biomolecule macromolecular is by after adsorption, depression Into these Minute pores, and then swallowed up；However, inventor thinks, those can allow the air point of miniature scale Son squeezes into the Minute pores therebetween, and being not equal to them also can directly allow that the large biological molecule of relative large scale enters, and two Person's difference on yardstick is huge, must not make sweeping generalizations.Explanation is bypassed, in any case, the biology of object is analyzed as dependence test Macromolecular is adsorbed by PDMS substrate micro-channels inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective The phenomenon of presence.
, can be from containment PDMS surfaces to life in order to prevent this PDMS substrate bodies relative to the effect of swallowing up of large biological molecule The absorption of thing macromolecular is addressed, and method is chemically modified modification aiming at the PDMS material surface, for For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described micro-channel part, by changing The micro-channel inner surface of modification is learned, its absorption to large biological molecule can be contained, and then avoid large biological molecule quilt PDMS substrate body phases are swallowed up；But, or that old problem, that is, the chemical modification on PDMS material surface changes Surface state after property can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface automatically, The process of migration, soon can become that micro-channel inner surface state being modified by surface chemical modification again script strong and dredge Water and the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS Its micro-channel inner surface of substrate is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, can reach again Growth stage contains absorption process of the PDMS substrate micro-channel inner surfaces to large biological molecule, and then prevents PDMS substrate body phases The effect of swallowing up to large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life, It is exactly a very intractable problem.The problem makes the numerous specialties in this area as another problem addressed above, equally Personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.The hardly possible Also be present many year in topic, so far, also not yet properly settled.
Third, as described above, the PDMS micro-channel inner surfaces are strongly hydrophobic, and targetedly surface chemical modification or come to the surface Learn modification to be difficult to again persistently, therefore, actually can only still belong to effective short in the surface state after the modification of its surface or surface modification It is used within phase；If have passed through that ofer short duration period of validity, and still by force if use, due to surface shape State already again close to hydrophobic state, is then certainly existed than larger flow resistance using usual Micropump driving test liquid stream, So, just test liquid can only be forced to flow to target direction flowing by increasing Micropump pump power and pumping pressure, as described above, This PDMS material is soft, pumps test liquid stream with too high, machinery pumping pressure, causing the substrate, it enters Bubbling, expanded, distortion, deformation, also, this high-pressure situations occur for the micro-channel that sample end includes sample introduction end near zone Under, also easily occur the stripping between substrate and cover plate, this feelings in the sample introduction end and its micro-channel of near zone and its periphery Under shape, sample solution flows in the crack of the appearance between the substrate and cover plate that are formed after the entrance stripping everywhere, this reality Border causes the damage of the micro-fluidic chip；Certainly, it is not in place if surface modification or surface modification script, also result in There is said circumstances within the of short duration usual term of validity；It is above-mentioned in the case where carrying out liquid stream driving using additional Micropump merely The problem exists all the time.If be modified or the pre action such as surface modification as described above, not doing any surface completely, So, the above-mentioned problem will be even more serious, even without occur micro-channel bubbling described in sample introduction end and its near zone, it is expanded, Between distortion, deformation and substrate and cover plate the problems such as peel off, merely because the flow resistance is excessive, using high pressure Micropump also not Test liquid stream must can be driven to be marched forward towards terminal side.
The content of the invention
The technical problems to be solved by the invention are to provide a package solution, while solving three addressed above The a series of problem actually mutually involved together of aspect, also, the solution is diagnosed applied to hypotype swine flu Multichannel micro-fluidic chip field is used, a kind of new hypotype swine flu diagnosis Multichannel device is formed.
The present invention solves the technical problem by following scheme, and the device that the program is provided is a kind of double drives containing hydrophobic substrate The hypotype swine flu multi-channel testing device of dynamic coupling, the structure of the device includes multichannel micro-fluidic chip, the micro-fluidic chip Structure include installing substrate and cover plate together bonded to each other, the substrate and cover plate are plate object or tablet, the base The channel structure via mould pressing process or etching technics formation is contained in that face towards the cover plate of piece, bonded to each other to be arranged on one The substrate risen has been built into the micro-fluidic chip containing pipeline configuration jointly with the cover plate, and the locations of structures of the pipeline is located at the base Piece and cover plate juncture area bonded to each other, the two ends of the pipeline connect with the sample introduction end of the micro-fluidic chip and terminal respectively Connect, the sample introduction end is the injection end of the micro-fluidic chip sample solution, when the terminal is the micro-fluidic chip actual sample introduction test its The terminal that sample solution flows in chip, the terminal is located remotely from each other with the sample introduction end, and the distance between the terminal and the sample introduction end are situated between Between 3 centimetres and 10 centimetres, working electrode is sequentially equiped with diverse location in the pipeline and to electrode and reference Electrode, the working electrode is resisted by conductive electrode and the hypotype swine flu specificity that embedded being attached on the conductive electrode The gold size sensitive membrane of body is constituted, and the construction of the pipeline is in parallel construction, the pipeline in parallel construction by three laterals simultaneously Connection is constituted, and the quantity of the working electrode is three, and the installation position of three working electrodes is located at three branched pipes respectively In road, and, the specific antibody in the sensitive membrane structure of its top layer gold size of three working electrodes is anti-to hypotype swine flu respectively Three kinds of hypotype swine flu antibody materials of proper energy specific binding, three kinds of antibody materials are hypotype swine flu specific antibody respectively H1N1、H3N2And H1N2, its material of the working electrode is gold material or thermal decomposition conducting polymer material, the working electrode Sheet or thread is presented in its pattern, and emphasis is that its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is former The surface of raw form, the surface of the primary form its be intended to refer to not pass through any surface chemical modification or any surface chemistry The surface of the primary form of the modified material, the structure of the device also includes chain ring type magnetic holding device, the chain ring type magnetic holding device Have six links in its structure, the link is in strip, shaft-like or bar-shaped, and each adjacent link is hinged to form chain link, The hexagonal cyclic structure of its appearance profile of the chain link, its locations of structures of each drift angle of the hexagonal annular structure is to be hinged knot Structure position, in the link of opposite side position, its material is magnetic conductivity material, the hexagon to two therein of the hexagonal annular structure Its material of the remaining four processes of cyclic structure is non-magnetic material, and the non magnetic material refers to not strong to externally-applied magnetic field Magnet exciting coil is wound with a link among the material of strong response, two links of the magnetic conductivity material, this is wound with excitation The link of coil is respectively equiped with the pole shoe of a magnetic conductivity material in the both sides neighbor positions of its magnet exciting coil, has described in two Pole shoe, two pole shoes are directed to paste on the link of another magnetic conductivity material, its surface of the link of another magnetic conductivity material Attached fixation is equiped with miniature ultrasonic transducer units, relies on the chain ring type magnetic holding device socket of electromagnetism adhesive strength to be positioned at the miniflow The close position of its terminal of chip is controlled, and, higher-order of oscillation electric signal transmission cable, higher-order of oscillation electric signal transmission electricity One end of cable links together with the miniature ultrasonic transducer units；The chain ring type magnetic holding device facilitates the device to disassemble there is provided one Function；Its major function of the miniature ultrasonic transducer units is, in the actual sample introduction test of micro-fluidic chip, to be launched using it Ultrasonic wave reduces the interfacial tension between sample solution and the inwall of its inner passage of micro-fluidic chip, can be compatible, Also, using the distance between the sample introduction end and the terminal and miniature ultrasonic transducer units installation position difference and its The difference on ultrasonic intensity experienced, its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension it Between difference, the interfacial tension difference between the micro-fluidic chip two ends can form pressure between the two ends of the micro-fluidic chip Power difference, the pressure gap can drive sample solution to the end flow；The miniature ultrasonic transducer units its functions also include with Its ultrasonic wave launched check in sample contained large biological molecule its on the micro-fluidic chip its inner passage inner surface Absorption, and then check swallow up effect of its body phase of the substrate of the dimethyl silicone polymer material to the large biological molecule；Softness is simultaneously Have the substrate of the dimethyl silicone polymer material its function of elasticity and include with its property to the strong absorption of ultrasonic wave, to ultrasonic wave Absorbed strongly, and ultrasound is thereby realized within micro-fluidic chip terminal to the limited short distance between the sample introduction end The rapid decrement of intensity of wave；And, Micropump, the Micropump is connected with the sample introduction end；The function of the Micropump is, in the sample solution Interfacial tension between the inwall of its inner passage of micro-fluidic chip is reduced by the ul-trasonic irradiation, the increase of alternate compatibility Precondition under, the interfacial tension come with the mechanicalness of Micropump pumping strength between the two ends that are induced the ultrasonic wave is poor Its different driving force brought is supported mutually, adjusts mutually, is mutually coupled, and one driving is pooled in the way of Collaboration Test liquid flows to the strength of the terminal direction flowing；Described its thickness of cover plate and substrate between 1.0 millimeters with 5.0 millimeters it Between；And, ultrasonic wave damper, its profile of ultrasonic wave damper is in block, tabular or bar-shaped；The ultrasonic wave damper its Material is microcellular rubber, sponge rubber or microporous polyurethane elastomer；And, elastic clip, the elastic force accompanies two clamping limbs, The medial surface of each clamping limb is relative with the medial surface of another clamping limb, and the medial surface of one of clamping limb is close to the miniflow The sample introduction end of chip is controlled, the medial surface of another clamping limb is close to the ultrasonic wave damper, and the elastic clip will with its gripping strength The sample introduction end of the micro-fluidic chip and the ultrasonic wave damper are clipped together bonded to each otherly, and the ultrasonic wave damper is with its material institute The strong consumption to ultrasonic wave brought, is further supported in the micro-fluidic chip terminal to limited between the sample introduction end Short distance within realize the rapid decrement of ultrasonic intensity, and thereby further expand described its interfacial tension of sample introduction end with it is described Difference between its interfacial tension of terminal.
The word of Micropump one art-recognized meanings of itself are known for the professional in micro-fluidic chip field.
The Micropump such as miniature piezoelectric pump, miniature peristaltic pump, miniature air driven pump, etc..
The Micropump both can be the Micropump of external form；The Micropump can also be done into or say inside the embedded micro-fluidic chip The Micropump of the built in version of its sample introduction end structure position or the sample introduction end Near-neighbor Structure position.
The word of magnet exciting coil one art-recognized meanings of itself are known.
The word of magnetic conductivity material one art-recognized meanings of itself are known；Belong to magnetic conductivity material material such as pure iron, Silicon steel, soft magnetic ferrite etc..
Non magnetic material described in this case refers to the material not responded strongly to externally-applied magnetic field, belongs to the non magnetic material Material such as metallic copper, metallic zinc, ormolu, metallic aluminium, almag etc..
The word art-recognized meanings of itself field in Machine Design that is hinged is known.
The gold size sensitive membrane is to be sufficiently mixed chitosan gold size solution and hypotype swine flu specific antibody solution uniformly, is used Point sample instrument point sample is coated on specified structure position, and forms its drying and forming-film.Hypotype swine flu in the gold size sensitive membrane Specific antibody is the hypotype swine flu antibody of horseradish peroxidase or glucose oxidase mark, the gold size sensitive membrane It has been included as fixing above-mentioned each hypotype swine flu specific antibody and introducing complementary medium therein, the complementary medium is for example Chitosan, cellulose acetate, gelatin be therein a kind of or their mixture.
The pipeline in the microfluidic chip structure includes the lateral, and its internal diameter size may each be arbitrarily selected Size, still, for less with the consideration in terms of prepare liquid sample and reduction reagent loss, the pipeline includes described as far as possible Lateral preferably selects the passage of capillary level, and the passage of the capillary level implies that its internal diameter and the capillary on ordinary meaning The suitable passage of the internal diameter of pipe.The shape of cross section of its inner passage of capillary can be arbitrary shape, the cross section Shape is for example circular, oval, square, rectangle, bar shaped, naturally it is also possible to be arbitrarily exist bending it is linear, also, The interior shape of the capillary is with the extension of pipeline, and the shape of cross section of different parts can also allow to be different shapes. Only for the word of capillary one, its art-recognized meanings is known.
What is be related in structure is the electrode of microsize to electrode and reference electrode, and its electrode shape may each be any choosing Fixed shape, the arbitrarily selected shape such as square piece shape, rectangular patch, strip or round sheet etc..It is described to electricity The art-recognized meanings of pole and the reference electrode vocabulary of itself are known.
It is public only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field Know.
Various sizes, variously-shaped ultrasonic transducer are commercially available；Commercially available miniature ultrasonic transducer units its sizes can be with It is small to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface itself and Speech, is known general technology for the professional in ultrasonic technology field.This case is not to this expansion superfluous words.
Only with regard to naked PDMS substrates itself micro-channel molding or lithographic technique for, be open-and-shut known technology.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the device can also include higher-order of oscillation electric signal generator；Its is another for the higher-order of oscillation electric signal transmission cable One end can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, for the professional in ultrasonic technology field, be It is simple and known；The higher-order of oscillation electric signal generator can be customized to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units be between 5 milliwatts and 9000 milliwatts it Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with Between 12MHz.
This case device can further include some annexes, the annex such as multiple tracks electrochemical workstation etc., institute certainly The art-recognized meanings for stating multiple tracks electrochemical workstation are known.Each working electrode for being related in this case microfluidic chip structure and , can be respectively via corresponding special the corresponding interface got lines crossed with the multiple tracks electrochemical workstation to electrode and reference electrode etc. Coupled.It is described that special to get lines crossed be for each the corresponding interface of each electrode and the multiple tracks electrochemical workstation is carried out into phase The private cable being mutually coupled with.The micro-fluidic chip in this case device, its structure can also include micro-valve, the number of the micro-valve Amount is not limited, according to actual needs, and the micro-valve can be installed in any required position installed in the microfluidic chip structure； The word of micro-valve one is for the professional of micro fluidic chip technical field, and the art-recognized meanings of itself are known；The micro-valve The manufacturing technology of itself and the use of technology is also known；The component that the micro-valve is not required.
The diameter of the working electrode can allow to be that any setting is easily installed the suitable diameter used, it is however recommended to Or say preferred its scope of the diameter between 0.1 micron to 2000 microns；The length of the working electrode can permit Permitted to be that any setting is easily installed the length used, it is however recommended to or to say preferred its scope of the length be at 1 micron To between 15000 microns.
The gold size for being installed in the working electrode surface layer by spraying or point sample instrument point sample or the coating of other appropriate process is quick Feel film, its thicknesses of layers can allow be any setting treat sample measuring liquid occur electrical signals response thickness, still, push away Preferred thickness is between 10 nanometers and 200 nanometers in other words for the thickness recommended.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, for example：Polypropylene, glass Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, such as do Realized into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and in the extremely short distance to ultrasonic wave Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip It is used as the cover plate using dimethyl silicone polymer, is also that this case technical scheme is allowed.
Described its thickness of cover plate and substrate can allow be any setting be easy to assembling thickness, the thickness of recommendation or say preferably Thickness be between 1.0 millimeters and 5.0 millimeters.Less thickness is conducive to saving material.
Described its size of ultrasonic wave damper is not limited, and described its length of ultrasonic wave damper, width, thickness may each be according to need The arbitrary size to be set.
It is known in field of rubber technology only for the word of microcellular rubber one art-recognized meanings of itself.
The microcellular rubber is commercially available.
It is known in field of rubber technology only for the word of sponge rubber one art-recognized meanings of itself.
The sponge rubber is commercially available.
It is known in technical field of macromolecules only for the word of microporous polyurethane elastomer one art-recognized meanings of itself.
The polyurethane micropore elastomer material is commercially available.
The microcellular rubber preferred microporous silicon rubber or micropore fluorubber.
It is known in field of rubber technology only for the word of micropore silicon rubber one art-recognized meanings of itself.
The microporous silicon Rubber market is on sale.
It is known in field of rubber technology only for the word of micropore fluorubber one art-recognized meanings of itself.
The micropore fluorubber is commercially available.
The word of elastic clip one art-recognized meanings of itself are known.
The elastic clip is commercially available.
The application method of this case micro-fluidic chip：
This case is flowed with dual drive coupling operating mode driving liquid stream in the capillary channel of the triple channel micro-fluidic chip, Three kinds of hypotype swine flu diagnostic antigens are detected respectively using multi-channel electrochemical analyzer device.
The specific detection of this case micro-fluidic chip is as follows using step：
1st, blood serum sample liquid is added in micro-pipe road, under dual drive coupling operating mode driving, various hypotype swine flus The hypotype pig for the corresponding horseradish peroxidase-labeled that antigen molecule is embedded by gold size sensitive membrane on electrode surface in each passage is flowed Feel specific antibody capture.
2nd, the hypotype swine flu antigen in the hypotype swine flu specific antibody and blood serum sample of horseradish peroxidase-labeled is formed Immune complex.
3rd, using multi-channel electrochemical analyzer, the electron mediators such as catechol are added, using the above-mentioned reaction of amperometric detection Caused curent change, is derived from the species and content of various analytes.
4th, result is subjected to comprehensive analysis, comprehensive diagnos is carried out to hypotype swine flu antigen.
It is an advantage of the invention that its close position relies on the socket of electromagnetism adhesive strength fixed in the terminal of the micro-fluidic chip Position described in chain ring type magnetic holding device, with the chain ring type magnetic holding device one magnetic conductivity link attaching installation miniature ultrasonic Wave transducer, the low-power launched using it, the ultrasonic wave of high-frequency band so that without surface chemical modification or come to the surface The compatibility learned between strong hydrophobic its tube wall of micro-fluidic chip internal pipeline and the test object aqueous solution of modification significantly increases Plus, this is test liquid stream by there is provided a realistic possibility；Meanwhile, using dimethyl silicone polymer substrate its to ultrasound The strong absorbability of ripple, in shorter distance, it is, from the terminal to only several centimeters the sample introduction end In the very short distance of yardstick, reach the rapid decrement of ultrasonic intensity, thereby cause institute at the two ends of the micro-fluidic chip The difference of interfacial tension is stated, the difference of the interfacial tension between the two ends can cause a kind of driving force, and this kind is poor because of interfacial tension Its function of driving force is to drive test liquid stream in strong hydrophobic capillary channel originally to the terminal side caused by different To flowing；And the simultaneous Micropump in structure, its function is, leads in the sample solution and the micro-fluidic chip inside it Interfacial tension between the inwall in road is reduced by the ul-trasonic irradiation, under the increased precondition of alternate compatibility, with the Micropump The two ends that are induced come the ultrasonic wave of mechanicalness pumping strength between interfacial tension difference its driving force for being brought Support mutually, adjust mutually, be mutually coupled, one driving test liquid is pooled in the way of Collaboration and flows to the terminal side To the strength of flowing；The presence of the Micropump so that its ultrasonic wave emissive porwer of the miniature ultrasonic transducer units can be allowed to appropriateness Reduction, this is particularly suitable for for the situation containing ultrasonic wave sensitive composition in detection object；And due to the ultrasonic transducer and its The presence of radiated ultrasonic wave, it is possible to increase alternate compatibility, reduce interfacial tension, and provide the two ends interfacial tension difference its The special driving force brought, then, in this case, its flow resistance in the micro-channel of test liquid stream significantly drops Low, correspondingly, its running resistance of the Micropump is greatly reduced, so, and the Micropump just can be to carry out than relatively low pumping pressure For the pumping work of the sample solution, because mechanical pumping pressure is greatly reduced, therefore, in such a situation, Just be not susceptible to because sample introduction terminal tool pumping pressure it is excessive caused by the sample introduction end and its near zone the micro-channel drum Between bubble, expansion, deformation, distortion and the region substrate and cover plate the problems such as stripping etc.；This case dual drive coupling running Scheme and add for the sample fluid flow act it is handling, can allow for using ultrasonic intensity, ultrasonic frequency, Micropump pump power, Micropump pumping pressure etc. multiple indexs include flowing forward to be directed to the flow rate of the flowing, flowing action Dynamic or pause flowing or acceleration flowing etc. flowing action carries out multi-parameter and accurately manipulated；By this case dual drive coupling running Scheme, entirely without must the substrate of the dimethyl silicone polymer material its micro-channel etc. relevant surfaces be carried out with any surface chemistry Modification or surface chemical modification, have altogether dispensed with the laborious procedures of the surface chemical modification or surface chemical modification；On the other hand, The ultrasonic wave of the low-power, high-frequency band, additionally it is possible to contain the large biological molecule in sample in the literalness naked poly dimethyl Absorption on its inner surface of pipeline of siloxanes substrate, and then contain its body phase of dimethyl silicone polymer substrate to described biological big point The effect of swallowing up of son；The Reversible binding thing of the antigen, antibody and antigen and antibody is all to belong to big point described of biology certainly The type of son；Because described suction-operated and the described effect of swallowing up effectively are contained, therefore, dependence test result will It is better able to objectively reflect actual conditions；The effect of the low-power, high-frequency band ultrasonic wave, certainly also include facilitate antigen, The quick of Reversible binding reaction between antibody is reached, and this causes dependence test operation can be with than the completion of faster speed.
As described above, the presence of the Micropump so that its ultrasonic wave emissive porwer of the miniature ultrasonic transducer units can be allowed to fit Degree reduction, then, the feature helps to protect its sensitive coating of the working electrode, is allowed to from ultrasound injury.
Based on this case scheme, repaiied completely without the surface chemistry carried out for the dimethyl silicone polymer substrate its relevant surfaces Decorations or surface chemical modification operation, therefore, this surface chemical modification layer or surface chemical modification layer not need to exist, So, its body phase interior polymer molecule of the dimethyl silicone polymer substrate is constantly diffused to the surface, migrated caused by it automatically Damaging influence to surface chemical modification layer or surface chemical modification layer is also just not present.
This case its installation position is located at the ultrasonic wave damper at the sample introduction end, from its terminal of this case micro-fluidic chip to entering Said on the sport technique segment that ultrasonic intensity rapid decrement is realized in the such short distance in sample end, the ultrasonic wave damper its function as The size expansion device of micro-fluidic chip, in other words, the ultrasonic wave damper its function as significantly extend it is micro-fluidic Its terminal of chip the distance between to sample introduction end, be the equal of from length of several centimetres of the extension of script to more than ten centimetres or Twenties centimetres of length or longer length, so so that described terminal is strong to the ultrasonic wave between sample introduction end in short distance The sport technique segment of degree rapid decrement can relatively easily be reached, also, the size expansion device namely the ultrasonic wave damper its Installation and removal are all very convenient, it is important that it can also be reused.
Its presence of the ultrasonic wave damper in this case device, further reduce to its pumping pressure of Micropump and pump power according to Rely；Correspondingly, its running resistance of the Micropump is further greatly reduced, so, and the Micropump just can be with lower pumping Pressure carries out the pumping work for the sample solution, because mechanical pumping pressure is further greatly reduced, Therefore, in such a situation, just be less susceptible to occur because sample introduction terminal tool pumping pressure it is excessive caused by the sample introduction end and The problems such as stripping between the micro-channel bubbling of its near zone, expansion, deformation, distortion and the region substrate and cover plate etc.； The scheme of this case dual drive coupling running is further added for the test liquid due to its presence of the ultrasonic wave damper The handling of flowing action is flowed, wherein, the factor that can adjust includes ultrasonic intensity, ultrasonic frequency, Micropump pump power, micro- Pump pumps pressure, and, the selection and use of ultrasonic wave damper material, the selection of ultrasonic wave damper block size and use, The selection of the size of its corresponding ultrasonic wave abatement ability of ultrasonic wave damper and use etc., this kind of many adjustable factor it is same When exist, can more operation indexs, further, more it is multifactor adjustably for the flowing flow rate, stream Action includes flow forward or pause flowing or accelerates flowing etc. flowing action to carry out the accurate manipulation under more parameters.
The technical scheme of this case has dissolved its application related one to dimethyl silicone polymer substrate addressed above totally Row technical barrier.Based on this case scheme, the polydimethyl siloxane material of this kind very cheap and easily processing and fabricating just has can Can in the micro-fluidic chip its prepare, production, using etc. field play bigger effect.
Fixation is attached in the chain ring type magnetic holding device one magnetic conductivity link in this case structure and has installed the miniature ultrasonic Transducer, the function that the device is disassembled is facilitated this architecture provides one, in this way, the chain ring type magnetic holding device is led together with one The miniature ultrasonic transducer units attached in magnetic link just easily can be mutually disengaged with the micro-fluidic chip, then, should The component that part can freely depart from is reused many times in which just can circulate benignly；The architectural feature is conducive to saving the device Use cost.
Brief description of the drawings
Fig. 1 be this case device chain ring type magnetic holding device its with outside side view rough under the micro-fluidic chip socket state, The legend and indicate the relative position relation under the socket state between each component.
Fig. 2 is the outside side view under another viewing angle of this case device；Fig. 2 viewing angle and Fig. 1 sight Angle is examined to be mutually perpendicular to；The components such as infusion tube and Micropump are depicted without in Fig. 2.
In figure, 1 is chain ring type magnetic holding device, and 2 be the terminal of the micro-fluidic chip, and 3 be the described of the micro-fluidic chip Sample introduction end, 4 be the substrate of dimethyl silicone polymer material, and 5 be cover plate, and 6 be miniature ultrasonic transducer units, and 7 be high frequency vibrating Electric signal transmission cable is swung, 8,15 be the link of two magnetic conductivity materials positioned at different structure position, two magnetic conductions respectively Property material link end-to-end relation each other in the structure shown here, 9,22 be two pole shoes positioned at different structure position respectively, 10, 12nd, 14,17,19,21 be six pin joints in six diverse locations respectively, and 11,13,18,20 be place respectively In the link of four non magnetic materials of four diverse locations, 16 be magnet exciting coil, and 23 be infusion tube, and 24 be Micropump；Figure The chain ring type magnetic holding device structure in example is only the legend structure of signal, and the actual chain ring type magnetic holding device structure is not limited to the figure Example chain ring type magnetic holding device structure；Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends Difference driving, the flow direction of its test liquid stream.The ultrasonic wave damper is depicted without in legend；Also the bullet is depicted without in legend Power is pressed from both sides.
Embodiment
In this case that Fig. 1 and Fig. 2 are shown embodiment, the structure of the device includes multichannel micro-fluidic chip, and this is micro- The structure of fluidic chip includes the substrate 4 and cover plate 5 of installing bonded to each other together, and the substrate 4 and cover plate 5 are tabular Contain the conduit knot via mould pressing process or etching technics formation in thing or tablet, that face towards the cover plate 5 of the substrate 4 Structure, substrate 4 bonded to each other being installed together has been built into the micro-fluidic chip containing pipeline configuration jointly with the cover plate 5, The locations of structures of the pipeline is located at the substrate 4 and the cover plate 5 juncture area bonded to each other, and the two ends of the pipeline are micro- with this respectively The sample introduction end 3 of fluidic chip and terminal 2 are connected, and the sample introduction end 3 is the injection end of the micro-fluidic chip sample solution, the end End 2 is the terminal of sample solution flowing in its chip when the actual sample introduction of the micro-fluidic chip is tested, the terminal 2 and the sample introduction end 3 It is located remotely from each other, the distance between the terminal 2 and the sample introduction end 3 are different in the pipeline between 3 centimetres and 10 centimetres Working electrode is sequentially equiped with position and to electrode and reference electrode, the working electrode is by conductive electrode and attaching The gold size sensitive membrane for having embedded hypotype swine flu specific antibody on the conductive electrode is constituted, and the construction of the pipeline is in parallel connection Construction, the pipeline in parallel construction is made up of three lateral parallel connections, and the quantity of the working electrode is three, and this three The installation position of individual working electrode is located in three laterals respectively, and, its top layer gold size of three working electrodes is quick The specific antibody felt in membrane structure is the three kinds of hypotype swine flu antibody things that can be specifically bound to hypotype swine flu antigen respectively Matter, three kinds of antibody materials are hypotype swine flu specific antibody H respectively1N1、H3N2And H1N2, its material of the working electrode is Sheet or thread is presented in gold material or thermal decomposition conducting polymer material, the working electrode its pattern, and emphasis is, the substrate 4 its material are dimethyl silicone polymer materials, and its surface of substrate 4 is the surface of primary form, the surface of the primary form its It is intended to refer to not by any surface chemical modification or the surface of the primary form of the material of any surface chemical modification, should The structure of device also include having in chain ring type magnetic holding device 1, its structure of the chain ring type magnetic holding device 1 six links 8,11, 13rd, 15,18,20, the link 8,11,13,15,18,20 is in strip, shaft-like or bar-shaped, each adjacent link It is hinged to form chain link, the hexagonal cyclic structure of its appearance profile of the chain link, each drift angle of the hexagonal annular structure 10th, 12,14,17,19,21 its locations of structures are articulated structure position, at two therein of the hexagonal annular structure In the link 8,15 of opposite side position its material be magnetic conductivity material, the remaining four processes 11 of the hexagonal annular structure, 13, 18th, 20 its material are non-magnetic materials, and the non magnetic material refers to the material not responded strongly to externally-applied magnetic field, Magnet exciting coil 16 is wound with a link 15 among two links of the magnetic conductivity material, this is wound with magnet exciting coil 16 Link 15 pole shoe of a magnetic conductivity material is respectively equiped with the both sides neighbor positions of its magnet exciting coil 16, have two The pole shoe 9,22, two pole shoes 9,22 are directed to the link 8 of another magnetic conductivity material, another magnetic conductivity material The fixed chain ring type electricity for being equiped with miniature ultrasonic transducer units 6, relying on electromagnetism adhesive strength is attached on its surface of the link 8 of matter Magnetic holding device 1 is socketed the close position for being positioned at its terminal 2 of the micro-fluidic chip, and, higher-order of oscillation electric signal transmission electricity Cable 7, one end of the higher-order of oscillation electric signal transmission cable 7 links together with the miniature ultrasonic transducer units 6；The chain ring type Magnetic holding device 1 facilitates the function that the device is disassembled there is provided one；Its major function of the miniature ultrasonic transducer units 6 is in miniflow When controlling the actual sample introduction of chip and testing, the ultrasonic wave launched using it reduces sample solution and its inner passage of the micro-fluidic chip Inwall between interfacial tension, can be compatible, also, using the sample introduction end 3 and the terminal 2 it is miniature with this Difference in the distance between the installation position of ultrasonic transducer 6 difference and its ultrasonic intensity experienced, induced synthesis Difference between its interfacial tension of described its interfacial tension of sample introduction end 3 and the terminal 2, the micro-fluidic chip two ends 2,3 Between interfacial tension difference can form pressure gap between the two ends 2,3 of the micro-fluidic chip, the pressure gap can drive Dynamic sample solution flows to the direction of terminal 2；Its function of the miniature ultrasonic transducer units 6 also includes the ultrasound launched with it Ripple checks in sample contained large biological molecule its absorption on the micro-fluidic chip its inner passage inner surface, and then checks Swallow up effect of its body phase of the substrate 4 of the dimethyl silicone polymer material to the large biological molecule；It is soft and have elasticity this poly- two The substrate 4 of methylsiloxane material its function includes, with its property to the strong absorption of ultrasonic wave, ultrasonic wave being inhaled strongly Receive, and thereby realize ultrasonic intensity within micro-fluidic chip terminal 2 to the limited short distance between the sample introduction end 3 Rapid decrement；And, Micropump 24, the Micropump 24 is connected with the sample introduction end 3；The function of the Micropump 24 is, in the examination Interfacial tension between sample solution and the inwall of its inner passage of micro-fluidic chip reduced by the ul-trasonic irradiation, alternate compatible Property increased precondition under, the two ends 2,3 for induce with the ultrasonic wave with the mechanicalness of the Micropump 24 pumping strength Between interfacial tension difference its driving force brought support mutually, adjust mutually, be mutually coupled, with the side of Collaboration Formula pools the strength that one driving test liquid flows to the direction of terminal 2 flowing；Its thickness of the cover plate 5 and substrate 4 is situated between Between 1.0 millimeters and 5.0 millimeters；And, ultrasonic wave damper, its profile of the ultrasonic wave damper in block, tabular or It is bar-shaped；Its material of the ultrasonic wave damper is microcellular rubber, sponge rubber or microporous polyurethane elastomer；And, elastic clip, The elastic force accompanies two clamping limbs, and the medial surface of each clamping limb is relative with the medial surface of another clamping limb, one of clamping The medial surface of arm is close to the sample introduction end 3 of the micro-fluidic chip, and the medial surface of another clamping limb is close to the ultrasonic wave damper, The elastic clip with its gripping strength by the sample introduction end 3 of the micro-fluidic chip and the ultrasonic wave damper it is bonded to each other clip together, The strong consumption to ultrasonic wave that the ultrasonic wave damper is brought with its material, further supporting should in the micro-fluidic chip Terminal 2 thereby further expands to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end 3 Difference between its interfacial tension of big described its interfacial tension of sample introduction end 3 and the terminal 2.
The ultrasonic wave damper is depicted without in legend；Also the elastic clip is depicted without in legend.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by two ends 2,3 pressure differentials drive, its try The flow direction of sample liquid stream.
The associate members such as the higher-order of oscillation electric signal generator are depicted without in Fig. 1 and Fig. 2.
Involved magnetic conductive material and involved nonmagnetic substance market are on sale.
Involved miniature ultrasonic transducer units 6 are commercially available；It can also be customized to ultrasonic transducer producer.
The Micropump 24 can be customized to specialized factory.
Its connected mode with various micro-fluidic chip of various Micropump is known.
Involved higher-order of oscillation electric signal transmission cable 7 is commercially available；Can also be to ultrasonic transducer producer or cable special manufacturer Family's customization.
Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also be customized to relevant manufacturers
Its inner passage of the micro-fluidic chip involved by this case is the pipeline of hydrophobic capillary form.
The word of Micropump one art-recognized meanings of itself are known for the professional in micro-fluidic chip field.
The Micropump such as miniature piezoelectric pump, miniature peristaltic pump, miniature air driven pump, etc..
The Micropump both can be the Micropump of external form；The Micropump can also be done into or say inside the embedded micro-fluidic chip The Micropump of the built in version of its sample introduction end structure position or the sample introduction end Near-neighbor Structure position, its basic structure key element and external form Micropump it is identical.Micropump in legend is the Micropump of external form.
Each working electrode in this example structure and electrode and reference electrode via each special cable or can be said respectively Get lines crossed respectively with the corresponding cable interface of the multiple tracks electrochemical workstation as annex or saying interface connection of getting lines crossed.
The structural form of the chain ring type magnetic holding device in legend is only an example, the knot of chain ring type magnetic holding device described in this case Configuration state is not limited to the structure type in the legend.

Claims (10)

1. the hypotype swine flu multi-channel testing device that the dual drive containing hydrophobic substrate is coupled, the structure of the device is micro- including multichannel Fluidic chip, the structure of the micro-fluidic chip includes the substrate and cover plate of installing bonded to each other together, and the substrate and cover plate are equal For plate object or tablet, the conduit via mould pressing process or etching technics formation is contained in that face towards the cover plate of the substrate Structure, substrate bonded to each other being installed together has been built into the micro-fluidic chip containing pipeline configuration jointly with the cover plate, should The locations of structures of pipeline is located at the substrate and cover plate juncture area bonded to each other, the two ends of the pipeline respectively with the micro-fluidic core The sample introduction end of piece and terminal connection, the sample introduction end is the injection end of the micro-fluidic chip sample solution, and the terminal is that this is micro-fluidic During the test of chip actual sample introduction in its chip sample solution flowing terminal, the terminal is located remotely from each other with the sample introduction end, the terminal and The distance between the sample introduction end is sequentially equiped with work electricity between 3 centimetres and 10 centimetres in the pipeline on diverse location Pole and to electrode and reference electrode, the working electrode is by conductive electrode and the embedding being attached on the conductive electrode The gold size sensitive membrane of hypotype swine flu specific antibody is constituted, and the construction of the pipeline is in parallel construction, described in parallel construction Pipeline is made up of three lateral parallel connections, and the quantity of the working electrode is three, the installation position point of three working electrodes Not Wei Yu in three laterals, and, the specific antibody in the sensitive membrane structure of its top layer gold size of three working electrodes It is the three kinds of hypotype swine flu antibody materials that can be specifically bound to hypotype swine flu antigen respectively, three kinds of antibody materials are respectively Hypotype swine flu specific antibody H1N1、H3N2And H1N2, its material of the working electrode is gold material or thermal decomposition conductive polymer Sheet or thread is presented in sub- material, its pattern of the working electrode, it is characterised in that its material of the substrate is polydimethylsiloxanes Alkane material, its surface of the substrate is the surface of primary form, the surface of the primary form its be intended to refer to not pass through any table The surface of the primary form of the material of face chemical modification or any surface chemical modification, the structure of the device also includes chain ring type electricity Six links are had in magnetic holding device, its structure of the chain ring type magnetic holding device, the link is in strip, shaft-like or bar-shaped, each phase Adjacent link is hinged to form chain link, the hexagonal cyclic structure of its appearance profile of the chain link, the hexagonal annular structure it is each Its locations of structures of drift angle is articulated structure position, and two therein of the hexagonal annular structure is in its material of the link of opposite side position Matter is magnetic conductivity material, and its material of the remaining four processes of the hexagonal annular structure is non-magnetic material, the non magnetic material Matter refers to winding in a link among the material for not responding externally-applied magnetic field strongly, two links of the magnetic conductivity material There is magnet exciting coil, this is wound with the link of magnet exciting coil and a magnetic conductivity is respectively equiped with the both sides neighbor positions of its magnet exciting coil The pole shoe of material, has two pole shoes, and two pole shoes are directed to the link of another magnetic conductivity material, this another lead Fixed chain ring type for being equiped with miniature ultrasonic transducer units, relying on electromagnetism adhesive strength is attached on its surface of the link of magnetic material Magnetic holding device is socketed the close position for being positioned at its terminal of the micro-fluidic chip, and, higher-order of oscillation electric signal transmission cable, One end of the higher-order of oscillation electric signal transmission cable links together with the miniature ultrasonic transducer units；The chain ring type magnetic holding device is carried A function of facilitating the device to disassemble is supplied；Its major function of the miniature ultrasonic transducer units is in the actual sample introduction of micro-fluidic chip During test, the ultrasonic wave launched using it reduces the boundary between sample solution and the inwall of its inner passage of micro-fluidic chip Face tension force, can be compatible, also, installs position with the miniature ultrasonic transducer units using the sample introduction end and the terminal The distance between put the difference on difference and its ultrasonic intensity experienced, its interfacial tension of sample introduction end described in induced synthesis With the difference between the terminal its interfacial tension, the interfacial tension difference between the micro-fluidic chip two ends can be micro-fluidic at this Pressure gap is formed between the two ends of chip, the pressure gap can drive sample solution to the end flow；The miniature ultrasonic Wave transducer its function also includes checking large biological molecule contained in sample with its ultrasonic wave launched that it is micro-fluidic at this Absorption on its inner passage inner surface of chip, and then it is big to the biology to check its body phase of the substrate of the dimethyl silicone polymer material The effect of swallowing up of molecule；Softness simultaneously has its function of the substrate of the dimethyl silicone polymer material of elasticity including strong to ultrasonic wave with it The property of strong absorption, is absorbed strongly to ultrasonic wave, and thereby in micro-fluidic chip terminal to having between the sample introduction end The rapid decrement of ultrasonic intensity is realized within the short distance of limit；And, Micropump, the Micropump is connected with the sample introduction end；The Micropump Function be that the interfacial tension between the inwall of the sample solution and its inner passage of micro-fluidic chip is by the ul-trasonic irradiation And reduce, under the increased precondition of alternate compatibility, the institute induced with the ultrasonic wave is come with the mechanicalness pumping strength of the Micropump State the interfacial tension difference between two ends its driving force brought to support mutually, adjust mutually, be mutually coupled, to cooperate with fortune The mode of work pools the strength that one driving test liquid flows to the terminal direction flowing；Described its thickness of cover plate and substrate is situated between Between 1.0 millimeters and 5.0 millimeters；And, ultrasonic wave damper, its profile of the ultrasonic wave damper in block, tabular or It is bar-shaped；Its material of the ultrasonic wave damper is microcellular rubber, sponge rubber or microporous polyurethane elastomer；And, elastic clip, The elastic force accompanies two clamping limbs, and the medial surface of each clamping limb is relative with the medial surface of another clamping limb, one of clamping The medial surface of arm is close to the sample introduction end of the micro-fluidic chip, and the medial surface of another clamping limb is close to the ultrasonic wave damper, The elastic clip with its gripping strength by the sample introduction end of the micro-fluidic chip and the ultrasonic wave damper it is bonded to each other clip together, should The strong consumption to ultrasonic wave that ultrasonic wave damper is brought with its material, is further supported at the micro-fluidic chip end The rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end is held, and is thereby further expanded described Difference between its interfacial tension of sample introduction end and the terminal its interfacial tension.
2. the hypotype swine flu multi-channel testing device of the dual drive coupling according to claim 1 containing hydrophobic substrate, it is special Levy and be, it is capillary channel that the pipeline, which includes the lateral,.
3. the hypotype swine flu multi-channel testing device of the dual drive coupling according to claim 1 containing hydrophobic substrate, it is special Levy and be, the thermal decomposition conducting polymer be by polyimides or polyacrylonitrile be heat-treated through anoxybiotic after the electric conductivity material that is formed Material.
4. the hypotype swine flu multi-channel testing device of the dual drive coupling according to claim 1 containing hydrophobic substrate, it is special Levy and be, the width or diameter of the working electrode between 0.1 micron to 2000 microns, and, the working electrode Length between 1 micron to 15000 microns.
5. the hypotype swine flu multi-channel testing device of the dual drive coupling according to claim 1 containing hydrophobic substrate, it is special Levy and be, the thickness of the gold size sensitive membrane is between 10 nanometers and 200 nanometers.
6. the hypotype swine flu multi-channel testing device of the dual drive coupling according to claim 1 containing hydrophobic substrate, it is special Levy and be, its material of the cover plate in structure is dimethyl silicone polymer material.
7. the hypotype swine flu multi-channel testing device of the dual drive coupling according to claim 1 containing hydrophobic substrate, it is special Levy and be, the Micropump is miniature piezoelectric pump, miniature peristaltic pump or miniature air driven pump.
8. the hypotype swine flu multi-channel testing device of the dual drive coupling according to claim 1 containing hydrophobic substrate, it is special Levy and be, the microcellular rubber is micropore silicon rubber or micropore fluorubber.
9. the hypotype swine flu multi-channel testing device of the dual drive coupling according to claim 1 containing hydrophobic substrate, it is special Levy and be, the structure of the device also includes higher-order of oscillation electric signal generator, its is another for the higher-order of oscillation electric signal transmission cable End is connected with the higher-order of oscillation electric signal generator.
10. the hypotype swine flu multi-channel testing device of the dual drive coupling according to claim 1 containing hydrophobic substrate, it is special Levy and be, its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 5 milliwatts and 9000 milliwatts, and this is miniature The frequency of its ultrasonic wave operationally launched of ultrasonic transducer is between 100KHz and 12MHz.
CN201610167662.7A 2016-03-08 2016-03-08 The hypotype swine flu multi-channel testing device of dual drive coupling containing hydrophobic substrate Withdrawn CN107167505A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102478582A (en) * 2011-10-08 2012-05-30 宁波大学 Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously
CN102645429A (en) * 2012-04-23 2012-08-22 宁波大学 Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device
CN204583216U (en) * 2015-03-19 2015-08-26 华南理工大学 A kind of micro-fluidic chip of microfluid spontaneous vasomotion and priming device
CN107138188A (en) * 2016-03-01 2017-09-08 李榕生 The hypotype swine flu detection means for the dual drive CGCM conveniently disassembled

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102478582A (en) * 2011-10-08 2012-05-30 宁波大学 Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously
CN102645429A (en) * 2012-04-23 2012-08-22 宁波大学 Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device
CN204583216U (en) * 2015-03-19 2015-08-26 华南理工大学 A kind of micro-fluidic chip of microfluid spontaneous vasomotion and priming device
CN107138188A (en) * 2016-03-01 2017-09-08 李榕生 The hypotype swine flu detection means for the dual drive CGCM conveniently disassembled

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
F. J. NAVARRO-BRULL等: "Guidelines for the design of efficient sono-microreactors", 《GREEN PROCESS SYNTH》 *
JAMES FRIEND ET AL.: "Microscale acoustofluidics: microfluidics driven via acoustics and ultrasonics", 《REVIEWS OF MODERN PHYSICS》 *

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Application publication date: 20170915