CN107362840A

CN107362840A - Using the micro flow control chip device of PDMS material substrates

Info

Publication number: CN107362840A
Application number: CN201610356790.6A
Authority: CN
Inventors: 李榕生
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-05-13
Filing date: 2016-05-13
Publication date: 2017-11-21

Abstract

The present invention relates to a kind of micro flow control chip device using PDMS material substrates, belong to analysis testing field.The substrate of micro-fluidic chip, some problems of physical presence are made with dimethyl silicone polymer i.e. PDMS that is cheap and easily processing.This case main points are, the selected PDMS with ecosystem surface of substrate, and the chain ring type magnetic holding device for being equiped with miniature ultrasonic transducer units socket is positioned at the sample liquid stream terminal of the micro-fluidic chip its neighbor positions, sample liquid stream is induced to be flowed from its sample introduction end of the chip along its pipeline of the chip to the terminal direction with ultrasonic wave, micron silica granule filler is filled up in its pipeline of the chip, its function of the filler includes supporting pipeline inwall, and, with the set of hydrophily fluid channel come the hydrophobic pipeline of compensatory script, and, extruding, cover inner-walls of duct face and thereby reduce the chance that large biological molecule contacts with the internal face.

Description

Using the micro flow control chip device of PDMS material substrates

Technical field

The present invention relates to a kind of micro flow control chip device using PDMS material substrates, belong to analysis testing field.

Background technology

For the microflow control technique overall general picture of itself, it may refer to famous micro-fluidic expert Mr. Lin Ping Cheng and go out not long ago Monograph " diagram Microfluid based Lab on a chip ", the monograph is published via Science Press, and the monograph is for microflow control technique Past, now, and, vision of the future etc. etc., suffer from detail, be deep into long discussion of detail.

The field that micro-fluidic chip is often applied to includes carrying out various analyses, detection to the sample solution containing large biological molecule.

The basic framework of micro-fluidic chip, including it is etched with the substrate of conduit and the cover plate to fit together therewith, the substrate On fluid course, before upper cover plate is assembled, it is apparent on see to be exactly some conduits, wait until cover cover plate thereon after, Just really closure forms the fluid course, and the conduit inner surface of the conduit is together with the part cover plate structure that surround the conduit Into described fluid course；So, it is clear that assemble the fluid course after completing, the main portion of its inner surface area Point it is the inner surface area of that conduit, in other words, the state or property of the conduit inner surface substantially determine that the liquid stream is led to The integrality or property in road；Therefore say, the inner surface state or inner surface property of this conduit of the structure on substrate are crucial Factor；In principle, any material that can be kept or keep its solid forms substantially, can be used to make substrate and lid Piece, such as, the material that can act as substrate and cover plate can be for example poly- diformazan of monocrystalline silicon piece, quartz plate, sheet glass, high polymer Radical siloxane, polymethyl methacrylate, makrolon etc.；Certainly, the selection of substrate and the selection of cover plate can with identical, It can also differ；From the point of view of material consumption, manufacture difficulty and application popularization prospect etc. etc., exist between these materials Not small difference, the especially selection of that substrate, have a great influence.

In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, so Substrate on to make conduit extremely simple, and the lower cost for material, substrate is made with the polydimethyl siloxane material, Conduit is suppressed or etched thereon, and is engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes, seemingly It is a kind of more satisfactory selection；Certainly, patch material can also select to use cheap polydimethyl siloxane material：So, This substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, and making is extremely simple, seems and should also be as being extremely easy to Popularization, promote.

But thing is really not so simple.

First, this polydimethyl siloxane material, that is, the material that alphabetical PDMS is referred to of abridging, itself are a kind of strong Hydrophobic material, conduit is built on this material, if without being operated for the modified of the conduit surface, then, it is overall After assembling is completed, that is, after covering cover plate, because the conduit its inner surface in structure is occupied in most fluid course Surface, then, its strong hydrophobic property of the PDMS conduits inner surface, is deciding factor, and it can be similar to water-soluble The fine liquid stream of polar liquid of liquid by becoming very difficult, its flow resistance is big, or even in general Micropump is all difficult to promote, Certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar；Therefore, existing Among having technology, modification is modified particular for the conduit inner surface on the PDMS material, is necessary operation；So, This is pretty troublesome for the modified operation of PDMS conduit inner surfacesThat falls nor this problem, forms serious technical puzzlement , it is another problem：PDMS polymer molecules inside its body phase of this PDMS material substrate, which have from trend surface, to be expanded Dissipate, the characteristic of migration, the characteristic that PDMS polymer molecules are diffused to the surface, migrated automatically inside this substrate body phase, will make The sufficiently long time must can not be maintained by the state after the modification of its inner surface of that conduit of surface modifying and decorating, that Holding time for conduit its inner surface state after surface-modified is substantially only sufficient to the time for completing laboratory internal test experiments Need；In other words, by surface is modified or the PDMS conduit inner surfaces of surface modification, after it is modified or modification is said The surface state formed afterwards can not be lasting, but soon automatically tends to or say the surface state before becoming surface modification again, The strong hydrophobic surface state of that script is returned in the shorter time, then, just think, such micro-fluidic chip energy Enough a large amount of making, mass storages, it is widely popularized, answer is it is obvious that is, impossible.On this PDMS material Conduit, if not doing surface modification, it can not pump and pass through similar to the fine liquid stream of polar solvent of the aqueous solution, chip also just cannot Use；And if having done surface modification, its state after modifying can not be persistently kept again, or equally can not popularization and application.

According to the literature, there is a kind of expedient solution, be that a small amount of surfactant is added in sample to be tested solution, with Just during test, dynamic, temporary transient effective surface hydrophilic layer is built temporarily in the PDMS conduits inner surface；However, Due to the amphipathic characteristic of surfactant, the introduced surfactant of the program is inevitable simultaneously also can be with being tested in sample solution Combination occurs for composition so that tested composition can not be detected normally or it is detected ratio and dubiously reduced；Live on the surface Property agent is possibly even fully wrapped around by tested composition with micelle form so that tested composition can not be detected or identify；Therefore, should Kind adds the scheme of surfactant into sample solution, is far from a preferable solution.

So, how to accomplish that substrate can either be made using cheap PDMS material, and the conduit inner surface can be released Decorating state can not persistently, chip can not largely make, largely lay in and then be widely popularized and such a make the numerous specialties in this area The puzzlement that personnel are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.

Be present many year in the highly difficult problem, so far, not yet properly settled.

Second, the PDMS material of non-surface modification, it is stated that, its surface is strongly hydrophobic above, this strong hydrophobic Material surface and also another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and And these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into it is gradually deep, directly It is trapped within the body phase of PDMS substrates to heavy, in fact, this process, is partly also due to inside PDMS material body phase Polymer molecule, which has, to be diffused to the surface, caused by travel motion；Such case, it can also be explained from another angle, i.e. Continuously from inside PDMS body phases to those polymer molecules of its diffusion into the surface, migration, its result moved, be by Gradually those are involved within the body phase of PDMS substrates by the large biological molecule of adsorption, briefly, these are inhaled Attached large biological molecule is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up large biological molecule Phenomenon, the influence caused by it, necessarily cause the severe deviations of all kinds of test data of experiment for being related to large biological molecule.

As described above, the problem of PDMS substrates, is, its not only adsorption large biological molecule, and swallow up large biological molecule, So, as the large biological molecule of experiment test object, its disappearance will not stop because surface saturation is adsorbed, but, no It is disconnected adsorbed, also constantly swallowed up.

On PDMS substrates in related experiment test process its body phase constantly swallow up test associated biomolecule macromolecular phenomenon, separately It is to say that one kind, which is explained, substantial amounts of Minute pores in PDMS body phases be present, and associated biomolecule macromolecular is by after adsorption, depression Into these Minute pores, and then swallowed up；However, inventor thinks, those can allow the air point of miniature scale Son squeezes into the Minute pores therebetween, not equal to saying that they also can directly allow the large biological molecule of relative large scale to enter, two Person's difference on yardstick is huge, must not make sweeping generalizations.Explanation is bypassed, in any case, the biology as dependence test analysis object Macromolecular is adsorbed by PDMS substrate conduits inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective deposits Phenomenon.

, can be from containment PDMS surfaces to life in order to prevent swallow up effect of this PDMS substrate bodies relative to large biological molecule The absorption of thing macromolecular addresses, and method is chemically modified modification aiming at the PDMS material surface, for For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described channel portion, by chemistry The conduit inner surface of modification, can contain its absorption to large biological molecule, and then avoid large biological molecule quilt PDMS substrate body phases are swallowed up；But or that old problem, that is, the chemical modification on PDMS material surface changes Surface state after property can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface automatically, The process of migration, soon it can become that conduit inner surface state being modified by surface chemical modification again script strong hydrophobic And the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS bases Its conduit inner surface of piece is always rapidly to strong hydrophobic surface state evolution.

So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, and can enough reaches Growth stage contains the absorption process of the PDMS substrate conduit inner surfaces to large biological molecule, and then prevents PDMS substrate bodies relative The effect of swallowing up of large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life, It is exactly a very intractable problem.The problem equally makes the numerous specialties in this area as another problem addressed above Personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.The hardly possible Also be present many year in topic, so far, also not yet properly settled.

Third, the polydimethyl siloxane material, namely PDMS material, the active force very little wherein between polymer molecule, its The polymer molecule of low polymerization degree inside body phase is in constantly into the dynamic process of surface migration, therefore, includes PDMS bases After it has been formed, the surface topography in its inner-walls of duct face of chip can be because of described low polymerization degree polymer molecule not for the chip of sheet material Migrate disconnectedly, pour into the surface and change, the change of its pattern is somewhat similarly to Rheological Deformation, trickling deformation or deformation of creep institute Pattern changes caused by possible；In the higher PDMS material of some low polymerization degree component ratios, this kind is somewhat similarly to flow The phenomenon of deformation, trickling deformation or the deformation of creep can show must be than more significant；Its shaping of chip of this kind comprising PDMS substrates The dynamic of inner-walls of duct surface topography afterwards changes property, namely the described property for being somewhat similarly to Rheological Deformation or trickling deformation Property or the deformation of creep property, this case applies mechanically the word of rheology one, integration, also referred to as the rheological equationm of state or Rheological Deformation property, The dynamic of corresponding inner-walls of duct face pattern changes, and this case is also referred to as rheology；As described above, because this kind of material its own The certain Rheological Deformation property as described above having, its manufactured goods actually can at leisure Rheological Deformation and cause the manufactured goods whole Individual structural form changes；Influence caused by its Rheological Deformation property, in terms of macroscopic perspective be it is subtle, still, For the very fine micro-fluidic chip of its internal structure, the rheological equationm of state can but cause bigger influence, therefore, in advance The fluid course namely the pipeline formed when first making, because the wall of its tube chamber of pipeline is mainly PDMS materials Wall, so, the cross-sectional area of the pipeline can actually taper into because of rheology over time, in other words, Due to the rheology, can more it become more for natively finer its tube chamber of the pipeline that liquid sample flows through Narrow, in the case of flow time is sufficiently long, or even the tube chamber of the pipeline can partly be closed or fully closed, and then be caused Sample fluid course is thoroughly blocked, the journey for closing or fully closing with being even also not reaching to its luminal part of pipeline Degree, its fluid passage that can be provided of that pipeline narrow because of rheology, then, in pipeline, its inner surface is exactly originally strong It is strong it is hydrophobic in the case of, aqueous sample liquid is just more difficult to by becoming narrower micro-channel so because of rheology, then, The influence of substrate rheology how is resisted, maintains the basic framework of the pipeline that gross distortion does not occur for a long time, also, keep the pipeline Long-term unobstructed, the problem of being exactly a urgent need to resolve.

The content of the invention

The technical problem to be solved by the invention is to provide a package solution, while solves the system addressed above Row problem.

The present invention solves the technical problem by following scheme, and the device that the program provides is that one kind uses PDMS material substrates Micro flow control chip device, the structure of the device includes micro-fluidic chip, and the structure of the micro-fluidic chip includes being bonded to each other installing Substrate and cover plate together, its test liquid of the micro-fluidic chip flows into sample end and the terminal of the sample fluid flow is located remotely from each other, Between 3 centimetres and 10 centimetres, emphasis is that its material of the substrate is poly- diformazan for the distance between the sample introduction end and the terminal Radical siloxane material, its surface of the substrate are the surfaces of primary form, the surface of the primary form its be intended to refer to and do not pass through The surface of the primary form of any surface chemical modification or the material of any surface chemical modification, the structure of the device also include chain Ring type magnetic holding device, shares six links in its structure of the chain ring type magnetic holding device, the link in strip, shaft-like or bar-shaped, Each adjacent link is hinged to form chain link, the hexagonal cyclic structure of its appearance profile of the chain link, the hexagonal annular structure Its locations of structures of each drift angle is articulated structure position, two therein links for being in opposite side position of the hexagonal annular structure Its material is magnetic conductivity material, and its material of the remaining four processes of the hexagonal annular structure is non-magnetic material, described non-magnetic Property material refers to the material not responded strongly to externally-applied magnetic field, in a link among two links of the magnetic conductivity material Magnet exciting coil is wound with, the link for being wound with magnet exciting coil is respectively equiped with one in the both sides neighbor positions of its magnet exciting coil and led The pole shoe of magnetic material, two pole shoes are shared, two pole shoes are directed to the link of another magnetic conductivity material, and this is another Fixed chain for being equiped with miniature ultrasonic transducer units, relying on electromagnetism adhesive strength is attached on its surface of the link of individual magnetic conductivity material Ring type magnetic holding device is socketed the close position for being positioned at its terminal of the micro-fluidic chip, and, the transmission of higher-order of oscillation electric signal Cable, one end and the miniature ultrasonic transducer units of the higher-order of oscillation electric signal transmission cable link together；The chain ring type electromagnetism Fixture provides a function of facilitating the device to disassemble；Its major function of the miniature ultrasonic transducer units is real in micro-fluidic chip During the sample introduction test of border, the sample introduction end and the distance between the terminal and the miniature ultrasonic transducer units installation position difference are utilized Difference on different and its ultrasonic intensity for being experienced, its interfacial tension of sample introduction end described in induced synthesis and its boundary of the terminal Difference between the tension force of face, interfacial tension difference between the micro-fluidic chip both ends can the both ends of the micro-fluidic chip it Between form pressure gap, the pressure gap can drive sample solution to the end flow；The poly dimethyl that is soft and having elasticity Its function of the substrate of siloxanes material includes, with its property to the strong absorption of ultrasonic wave, absorbing ultrasonic wave strongly, and by This realizes the rapid decrement of ultrasonic intensity within micro-fluidic chip terminal to the limited short distance between the sample introduction end； And many silica dioxide granules, many silica dioxide granules are filled in the pipeline of the micro-fluidic chip, the pipe Its tube chamber of road is filled up by many silica dioxide granules, and its particle size range of the silica dioxide granule is between 10 microns and 200 Between micron, the silica dioxide granule is silica crystalline particles, silica glass granule or silica composition shared by it Inorganic glass particles of the percentage by weight more than 80%.

Its particle diameter of the silica dioxide granule can be that the basis between 10 microns and 200 microns is actually needed and is arbitrarily designated Particle diameter, such as 10 microns, 30 microns, 70 microns, 100 microns, 150 microns or 200 microns of the particle diameter, etc..

The scope of further preferred its particle diameter of the silica dioxide granule is between 100 microns and 200 microns.

Its pattern of the silica dioxide granule is unlimited, and the silica dioxide granule for example can be spheric granules, rod-shaped particles, cuboid Shape particle or any amorphous granular.

The various silica dioxide granules manufacturing technology of itself is known technology.

Various forms, the silica dioxide granule market of all size are on sale.

The silica dioxide granule can also customize to specialized factory.

The preferred scope of its internal diameter of pipeline is between 500 microns and 1000 microns；It is but preferred compared to above-mentioned Internal diameter of the pipeline scope, more tiny internal diameter of the pipeline or thicker internal diameter of the pipeline are also what this case was allowed.

The word of magnet exciting coil one art-recognized meanings of itself are known.

The word of magnetic conductivity material one art-recognized meanings of itself are known；Belong to magnetic conductivity material material such as pure iron, Silicon steel, soft magnetic ferrite etc..

Non magnetic material described in this case refers to the material not responded strongly to externally-applied magnetic field, belongs to the non magnetic material Material such as metallic copper, metallic zinc, ormolu, metallic aluminium, almag etc..

The be hinged word art-recognized meanings of itself field in Machine Design is known.

It is public only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field Know.

Various sizes, variously-shaped ultrasonic transducer are commercially available；Commercially available miniature ultrasonic transducer units its sizes can be with It is small to arrive the magnitude only calculated with millimeter.

Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface itself and Speech, is known general technology for the professional in ultrasonic technology field.This case is not to this expansion superfluous words.

Only with regard to naked PDMS substrates itself conduit molding or lithographic technique for, be open-and-shut known technology；Equally Ground, the technology of hole-opening is even more known simple technique on naked PDMS substrates.This case is not also to this expansion superfluous words.

The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.

The structure of the device can also include higher-order of oscillation electric signal generator；Its is another for the higher-order of oscillation electric signal transmission cable One end can be connected with the higher-order of oscillation electric signal generator.

The involved higher-order of oscillation electric signal generator technology of itself, for the professional in ultrasonic technology field, it is It is simple and known；The higher-order of oscillation electric signal generator can customize to ultrasonic instrument specialized factory.

The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units be between 2 milliwatts and 9000 milliwatts it Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with Between 12MHz.

This case device can further include some annexes certainly, and the annex can be for example used cooperatively with micro-fluidic chip Electrically or optically detection device.The equipment that the electricity, optics etc. are used cooperatively with micro-fluidic chip, it may refer to described Monograph.

The cover plate in chip structure, its material can allow to be any electrical insulating property material, such as：Polypropylene, glass Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, for example do Into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and realized in the extremely short distance to ultrasonic wave Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip Using dimethyl silicone polymer it is used as the cover plate, and this case technical scheme is allowed.

Described its thickness of cover plate and substrate can allow be any setting be easy to assembling thickness, the thickness of recommendation or say preferably Thickness be between 1.0 millimeters and 5.0 millimeters.Less thickness is advantageous to save material.

It is an advantage of the invention that its close position relies on the socket of electromagnetism adhesive strength fixed in the terminal of the micro-fluidic chip Chain ring type magnetic holding device described in position, installation miniature ultrasonic is attached in the chain ring type magnetic holding device one magnetic conductivity link and is changed Energy device, with the ultrasonic wave of miniature ultrasonic transducer units transmitting low-power, high-frequency band, meanwhile, utilize dimethyl silicone polymer Its strong absorbability to ultrasonic wave of substrate, in shorter distance, it is, from the terminal to the sample introduction end it Between only several centimeters yardsticks very short distance in, reach the rapid decrement of ultrasonic intensity, thereby in the institute of the micro-fluidic chip State the difference that the interfacial tension is caused at both ends, and then, using the interfacial tension between the both ends difference its formed this two Pressure gap between end, driving sample liquid stream flow in the pipeline to the terminal direction.By this case liquid stream driving side Case, the equipment of traditional Micropump etc is altogether dispensed with；The micro-fluidic chip of Micropump is eliminated, its structure is more succinct, Its production process is less, and its cost of manufacture is lower, and this kind of succinct structure is more beneficial for the low cost application for reaching micro-fluidic chip.

Its scheme of this case and by the pipeline except electrode installation take up space in addition to all clearance spaces all use described in Silica dioxide granule fills up.Those are filled in the silica dioxide granule in pipeline, and this case is filled out to its overall abbreviation silica dioxide granule Fill thing, its function includes support, withstands the tube chamber of the pipeline its inwall so that the tube chamber of the pipeline from because of the rheology and Narrow, prevent its reduced cross-sectional area of the tube chamber of the pipeline or even close, so as to maintain the basic framework of the pipeline not send out for a long time Raw serious deformation.

Numerous silica dioxide granule entities are mutually banked up under this case framework together at random in the pipeline, the silica The function of grain filler, also includes with the hydrophilic surface of its silica dioxide granule, utilizes each adjacent silicon dioxide particle certainly Mutual formed complications are continuous and its inner surface that is mutually close together by water-wetted surface has the sky of hydrophilic nmature Gap, in the hydrophilic fluid channel of the pipeline Inner Constitution network morphology, the having all the time although this complications can be continuous The fluid channel of hydrophilic nmature, then, inside the tube chamber of the pipeline simultaneously and a plurality of fluid channel deposited its synthesis, The effect of integrated, cumulative superposition in other words, is the equal of the relatively fine hydrophilic capillary channel of caliber；The complications Its presence of continuous hydrophilic fluid channel, counteracts its surface of the PDMS substrates of the primary form to a large extent The flow resistance for liquid sample caused by strong hydrophobic property；In other words, because of liquid sample its account for maximum ratio into It is water to divide, and liquid sample is substantially exactly the aqueous solution, and therefore, the presence of the silica dioxide granule filler can be significantly Overcome with the unmodified PDMS substrates of hydrophobic property and surface its to the aqueous solution it is incompatible, repel and barrier effect, And then be greatly reduced belong to properties of Aqueous Solution sample liquid stream its be advanced through the resistance of pipeline.

Under this case framework, inside the tube chamber of the pipeline, the area of its lumen wall wall of former pipeline, because of the titanium dioxide The rigid of silicon grain filler ties up, on lumen wall wall still can also be exposed its face of the surface of part hydrophobic property Product is already dramatically reduced, and the area on its still exposed part surface for belonging to hydrophobic property does not have far smaller than The wall surface area of the hydrophobic property of the lumen wall wall in the presence of silica dioxide granule filler；So, at this Under the case framework, in its lumen of the pipeline, its remaining hydrophobic surface increases surface newly with hydrophilic part, that it is superimposed, comprehensive , summation, cumulative technique effect, be to form the surface for tending to be hydrophilic on the whole；In other words, in the pipeline Inside tube chamber, its interfacial tension between sample solution of the part surface of remaining hydrophobic property, plus that of hydrophilic nmature Newly-increased its interfacial tension between sample solution of surface in part, that it is superimposed, comprehensive, summation, cumulative technique effect, Be so that its solid-liquid interfacial tension inside the tube chamber of the pipeline synergy level off to its tube chamber inner surface of capillary glass tube with Interfacial tension between sample solution.

Therefore, exist in the pipeline under this case framework for having stated silica dioxide granule filler, while rely on this case sample Liquid stream drive scheme, it becomes possible to the driving to sample liquid stream is reached with relatively low ultrasonic power, with the examination of this case mechanism drives Sample liquid flows to the end flow.

On the other hand, due to its rheological equationms of state of the PDMS as substrate material, it is filled in the pipeline The silica dioxide granule filler, can gradually by constantly Rheological Deformation and to arest neighbors free space promote PDMS The pipeline wall of material more tightly wraps up, and this process eventually causes those part dioxies for pressing close to the lumen wall wall Silicon carbide particle is stuck in original place or is stuck in original place or embedding in-situ silica dioxide granule and remaining embedded in original place, the part The silica dioxide granule banked up with being mutually closely packed together each other incarceration together, due to this reason, the titanium dioxide Silicon grain filler just will not move in the pipeline easily, and silica dioxide granule therein, which is also substantially all, is locked in original Locate, therefore the stream framework of the micro-fluidic chip can be kept for a long time.

The particle size range of its particle of this case silica dioxide granule filler is between 10 microns and 200 microns, compared to general For organic molecule, general large biological molecule, the particle diameter of its particle of this case silica dioxide granule filler can be rated as it is huge, by In its huge particle diameter, and the inner surface of the surface of its polarity and the pipeline of strong hydrophobic PDMS materials can not be compatible Or say and mutually melt, then, the huge silica dioxide granule of this kind of particle diameter can not be by the interior table of the pipeline of PDMS materials Face is adsorbed, and can not more be swallowed up by the inner surface of the pipeline of PDMS materials, the huge titanium dioxide of this kind of particle diameter Silicon grain surely not be fully sunk in easily or sink to completely PDMS materials its among intrinsic many Minute pores, and And because above it is stated that the rheology the reason for, the silica dioxide granule filler can be stuck in original place in other words it is embedding In original place without moving easily.

Inside the tube chamber of the pipeline, its lumen wall wall of former pipeline is entirely the surface of hydrophobic property originally, as described above, Under this case framework, rigid because of the silica dioxide granule filler ties up, still can also be exposed on lumen wall wall Its area of the surface of part hydrophobic property be already dramatically reduced, its still exposed part for belonging to hydrophobic property The area on surface be far smaller than no silica dioxide granule filler in the presence of the lumen wall wall dredge The wall surface area of aqueous nature；The reason for because of the rheology, it is close to those silica dioxide granules of lumen wall wall, gradually Ground is partially embedded into or is partly absorbed among the lumen wall wall, its direction pipe of the silica dioxide granule among the state The part surface of cavity wall wall is with having occurred and that the lumen wall wall of matching deformation is brought into close contact, then, in tube chamber The part wall that the matching is deformed and is brought into close contact with silica particles of having occurred and that in wall wall is actually It can not be contacted with flowing through the sample solution of pipeline, that is to say, that the wall of this part can not be to flowing through the examination of pipeline Sample solution large biological molecule therein and organic molecule produce suction-operated.Based on above-mentioned mechanism, then because filling up described Its presence of the silica dioxide granule filler of pipeline, the overwhelming majority of its lumen wall wall of the pipeline are tightly hidden Lid, these described walls tightly covered can not contact with flowing through the sample solution of the pipeline, be only remainder The hydrophobic surface for not being brought into close contact by its surface of silica dioxide granule, tightly covering of fraction may be connect with sample solution Touch, in other words, under this case framework, large biological molecule and organic molecule in sample solution its with hydrophobic PDMS walls Direct touch opportunity significantly reduce, thus, the lumen wall faces of PDMS materials its to large biological molecule and organic molecule Adsorptive hindrance and swallow up interference be greatly reduced；As described above, this case scheme is advantageous to exclude or weakens the absorption Interference is disturbed and swallowed up, is advantageous to be lifted the reliability of correlation analysis test data.

As described above, this case silica dioxide granule filler, its function actually includes being similar to the pipe for PDMS materials Road inner surface carries out the effect of chemical modification, and functioning as its this respect of this case is by the inner surface of pipeline of the PDMS materials By the surface modification of hydrophobic property into the surface of hydrophilic nmature, still, with general, usual PDMS surface chemical modifications its The hydrophilic chemical decorative layer retention time is short, hydrophilic effect can not keep that situation prolonged enough is different, and particle described in this case is huge Silica dioxide granule filler, its particle being stuck by rheology can not both be moved easily, and the particle of its huge particle diameter is more It can not be swallowed up easily by the inner-walls of duct of PDMS materials, therefore, its from this respect is similar to the technology of surface chemical modification Seen in effect, its technique effect of this case, be form it is permanent, can not erase, can not consume, not be corroded, not swallowed up , the hydrophilic surface reforming layer being not dissolved, it is just comparable to a kind of in PDMS base materials its relevant surfaces in effect The permanent hydrophilic modifying superficial layer of structure.

The technical scheme of this case dissolved totally address above to dimethyl silicone polymer substrate its apply related system Row technical barrier.Based on this case scheme, the very cheap polydimethyl siloxane material of this kind is just possible in the micro-fluidic chip Prepare, production, using etc. field play bigger effect.

Fixation is attached in the chain ring type magnetic holding device one magnetic conductivity link in this case structure and has installed the miniature ultrasonic Transducer, this architecture provides a function of facilitating the device to disassemble, in this way, the chain ring type magnetic holding device is led together with one The miniature ultrasonic transducer units attached in magnetic link just easily can be mutually disengaged with the micro-fluidic chip, then, the portion The component that dividing freely to depart from is reused many times in which just can circulate benignly；The architectural feature is advantageous to save making for the device Use cost.

Brief description of the drawings

Fig. 1 be this case device chain ring type magnetic holding device its with outside side view rough under the micro-fluidic chip socket state, The legend and indicate the relative position relation under the socket state between each component.

Fig. 2 is the outside side view under another viewing angle of this case device；Fig. 2 viewing angle and Fig. 1 sight Angle is examined to be mutually perpendicular to.

In figure, 1 is chain ring type magnetic holding device, and 2 be the terminal of the micro-fluidic chip, and 3 be the described of the micro-fluidic chip Sample introduction end, 4 be the substrate of dimethyl silicone polymer material, and 5 be cover plate, and 6 be miniature ultrasonic transducer units, and 7 be high frequency vibrating Swing electric signal transmission cable, 8,15 be the link of two magnetic conductivity materials positioned at different structure position respectively, two magnetic conductions Property material link end-to-end relation each other in the structure shown here, 9,22 be two pole shoes positioned at different structure position respectively, 10, 12nd, 14,17,19,21 be six pin joints in six diverse locations respectively, and 11,13,18,20 be place respectively In the link of four non magnetic materials of four diverse locations, 16 be magnet exciting coil；The chain ring type electromagnetic clamp in legend is signed an undertaking Structure is only the legend structure of signal, and the actual chain ring type magnetic holding device structure is not limited to the legend chain ring type magnetic holding device structure；Figure Example in arrow indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, the flowing of its sample liquid stream Direction.

Embodiment

In this case that Fig. 1 is shown embodiment, the structure of the device includes micro-fluidic chip, the knot of the micro-fluidic chip Structure includes being bonded to each other the substrate 4 and cover plate 5 of installing together, and its test liquid of the micro-fluidic chip flows into sample end 3 and the sample The terminal 2 of fluid flow is located remotely from each other, the distance between the sample introduction end 3 and the terminal 2 between 3 centimetres and 10 centimetres, Emphasis is, its material of the substrate 4 is dimethyl silicone polymer material, and its surface of substrate 4 is the surface of primary form, the original The surface of raw form its be intended to refer to not by any surface chemical modification or the original of the material of any surface chemical modification The surface of raw form, the structure of the device also include chain ring type magnetic holding device 1, shared in its structure of the chain ring type magnetic holding device 1 Six links 8,11,13,15,18,20, the link 8,11,13,15,18,20 in strip, it is shaft-like or Bar-shaped, each adjacent link is hinged to form chain link, the hexagonal cyclic structure of its appearance profile of the chain link, the hexagonal annular Its locations of structures of each drift angle 10,12,14,17,19,21 of structure is articulated structure position, the hexagonal annular knot Two therein of structure is magnetic conductivity material in the link 8,15 of opposite side position its material, the remainder four of the hexagonal annular structure Individual its material of link 11,13,18,20 is non-magnetic material, and the non magnetic material refers to not strong to externally-applied magnetic field The material of strong response, magnet exciting coil 16 is wound with a link 15 among two links of the magnetic conductivity material, the winding The link 15 for having magnet exciting coil 16 is respectively equiped with the pole of a magnetic conductivity material in the both sides neighbor positions of its magnet exciting coil 16 Boots, two pole shoes 9,22 are shared, two pole shoes 9,22 are directed to the link 8 of another magnetic conductivity material, and this is another Attach to fix on its surface of the link 8 of one magnetic conductivity material and be equiped with miniature ultrasonic transducer units 6, rely on electromagnetism adhesive strength The chain ring type magnetic holding device 1 socket be positioned at the close position of its terminal 2 of the micro-fluidic chip, and, the higher-order of oscillation Electric signal transmission cable 7, one end of the higher-order of oscillation electric signal transmission cable 7 are connected to one with the miniature ultrasonic transducer units 6 Rise；The chain ring type magnetic holding device 1 provides a function of facilitating the device to disassemble；Its is main for the miniature ultrasonic transducer units 6 Function is in the actual sample introduction test of micro-fluidic chip, is changed using the sample introduction end 3 and the terminal 2 with the miniature ultrasonic Difference in the distance between the energy installation position of device 6 difference and its ultrasonic intensity experienced, sample introduction described in induced synthesis Hold the difference between 3 its interfacial tension and the terminal 2 its interfacial tension, the boundary between the micro-fluidic chip both ends 2,3 Face tension difference can form pressure gap between the both ends 2,3 of the micro-fluidic chip, and the pressure gap can drive sample molten Liquid flows to the direction of terminal 2；Softness simultaneously has its function of the substrate 4 of the dimethyl silicone polymer material of elasticity including with it To the property of the strong absorption of ultrasonic wave, ultrasonic wave is absorbed strongly, and thereby in the micro-fluidic chip, the terminal 2 is entered to this The rapid decrement of ultrasonic intensity is realized within limited short distance between sample end 3；And many silica dioxide granules, Many silica dioxide granules are filled in the pipeline of the micro-fluidic chip, and its tube chamber of the pipeline is by many silica Particle is filled up, and between 10 microns and 200 microns, the silica dioxide granule is its particle size range of the silica dioxide granule Its shared percentage by weight of silica crystalline particles, silica glass granule or silica composition is inorganic more than 80% Glass particle.

Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its test liquid The flow direction of stream.

The associate members such as the higher-order of oscillation electric signal generator are depicted without in Fig. 1 and Fig. 2.

Involved magnetic conductive material and involved nonmagnetic substance market are on sale.

Involved miniature ultrasonic transducer units 6 are commercially available；It can also be customized to ultrasonic transducer producer.

Involved higher-order of oscillation electric signal transmission cable 7 is commercially available；Can also be to ultrasonic transducer producer or cable special manufacturer Family's customization.

Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also be customized to relevant manufacturers.

Claims

1. using the micro flow control chip device of PDMS material substrates, the structure of the device includes micro-fluidic chip, the micro-fluidic chip Structure include being bonded to each other installing substrate and cover plate together, its test liquid of the micro-fluidic chip flows into sample end and the test liquid The terminal of stream flowing is located remotely from each other, and the distance between the sample introduction end and the terminal are between 3 centimetres and 10 centimetres, its feature It is, its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the primary form Surface its be intended to refer to the primary form of the not material by any surface chemical modification or any surface chemical modification Surface, the structure of the device also includes chain ring type magnetic holding device, and six links are shared in its structure of the chain ring type magnetic holding device, The link is in strip, shaft-like or bar-shaped, and each adjacent link is hinged to form chain link, and its appearance profile of the chain link is in six sides Shape cyclic structure, its locations of structures of each drift angle of the hexagonal annular structure is articulated structure position, the hexagonal annular knot Two therein of structure is magnetic conductivity material in the link of opposite side position its material, the remaining four processes of the hexagonal annular structure Its material is non-magnetic material, and the non magnetic material refers to the material not responded strongly to externally-applied magnetic field, the magnetic conductivity Magnet exciting coil is wound with a link among two links of material, this is wound with the link of magnet exciting coil in its magnet exciting coil Both sides neighbor positions on be respectively equiped with the pole shoe of a magnetic conductivity material, share two pole shoes, two pole shoes are directed to The link of another magnetic conductivity material, fixation is attached on its surface of the link of another magnetic conductivity material and is equiped with miniature ultrasonic Transducer, the chain ring type magnetic holding device socket of electromagnetism adhesive strength is relied on to be positioned at the neighbouring of the micro-fluidic chip its terminal Position, and, higher-order of oscillation electric signal transmission cable, one end and the miniature ultrasonic of the higher-order of oscillation electric signal transmission cable Transducer links together；The chain ring type magnetic holding device provides a function of facilitating the device to disassemble；The miniature ultrasonic changes Can device its major function be in the actual sample introduction test of micro-fluidic chip, it is miniature super with this using the sample introduction end and the terminal Difference in the distance between acoustic wave transducer installation position difference and its ultrasonic intensity experienced, described in induced synthesis Difference between its interfacial tension of sample introduction end and the terminal its interfacial tension, the interfacial tension between the micro-fluidic chip both ends Difference can form pressure gap between the both ends of the micro-fluidic chip, and the pressure gap can drive sample solution to the terminal Flowing；Softness simultaneously has its function of the substrate of the dimethyl silicone polymer material of elasticity including with its property to the strong absorption of ultrasonic wave Matter, ultrasonic wave is absorbed strongly, and thereby in micro-fluidic chip terminal to the limited short distance between the sample introduction end Within realize the rapid decrement of ultrasonic intensity；And many silica dioxide granules, many silica dioxide granule fillings In the pipeline of the micro-fluidic chip, its tube chamber of the pipeline is filled up by many silica dioxide granules, the silica For its particle size range of grain between 10 microns and 200 microns, the silica dioxide granule is silica crystalline particles, titanium dioxide Silica glass particle or silica composition its inorganic glass particles of shared percentage by weight more than 80%.

2. the micro flow control chip device according to claim 1 using PDMS material substrates, it is characterised in that the cover plate Its material is dimethyl silicone polymer material.

3. the micro flow control chip device according to claim 1 using PDMS material substrates, it is characterised in that the cover plate And substrate its thickness is between 1.0 millimeters and 5.0 millimeters.

4. the micro flow control chip device according to claim 1 using PDMS material substrates, it is characterised in that the device Structure also includes higher-order of oscillation electric signal generator, its other end of the higher-order of oscillation electric signal transmission cable and higher-order of oscillation electricity Signal generator connects.

5. the micro flow control chip device according to claim 1 using PDMS material substrates, it is characterised in that this is miniature super Its specified ultrasonic wave transmission power of acoustic wave transducer between 2 milliwatts and 9000 milliwatts, the miniature ultrasonic transducer units its The frequency for the ultrasonic wave launched during operation is between 100KHz and 12MHz.

6. the micro flow control chip device according to claim 1 using PDMS material substrates, it is characterised in that the titanium dioxide Its particle size range of silicon grain is between 100 microns and 200 microns, and its inside diameter ranges of the pipeline are between 500 microns and 1000 Between micron.