CN107362842A - The cholera diagnosis micro fluidic device of quick removal is capable of in sample liquid driving with component - Google Patents
The cholera diagnosis micro fluidic device of quick removal is capable of in sample liquid driving with component Download PDFInfo
- Publication number
- CN107362842A CN107362842A CN201610356871.6A CN201610356871A CN107362842A CN 107362842 A CN107362842 A CN 107362842A CN 201610356871 A CN201610356871 A CN 201610356871A CN 107362842 A CN107362842 A CN 107362842A
- Authority
- CN
- China
- Prior art keywords
- micro
- pipeline
- cholera
- substrate
- fluidic chip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Dispersion Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Electrochemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The present invention relates to the cholera diagnosis micro fluidic device that a kind of sample liquid driving component is capable of quick removal, belong to analysis testing field.Serial problem be present if for making the substrate of cholera diagnosis micro-fluidic chip in dimethyl silicone polymer i.e. PDMS that is cheap and easily processing.This case main points are, the selected PDMS with ecosystem surface of substrate, and the chain ring type magnetic holding device for being equiped with miniature ultrasonic transducer units socket is positioned at the sample liquid stream terminal of the micro-fluidic chip its neighbor positions, sample liquid stream is induced to be flowed from its sample introduction end of the chip along its pipeline of the chip to the terminal direction with ultrasonic wave, micron silica granule filler is filled up in its pipeline of the chip, its function of the filler includes supporting pipeline inwall, and, with the set of hydrophily fluid channel come the hydrophobic pipeline of compensatory script, and, extruding, cover inner-walls of duct face and thereby reduce the chance that large biological molecule contacts with the internal face.
Description
Technical field
The present invention relates to the cholera diagnosis micro fluidic device that a kind of sample liquid driving component is capable of quick removal, belong to
Analyze testing field.
Background technology
The micro-fluidic cholera diagnostic techniques background of associated multi-channel, may refer to the patents of invention such as CN 200910150930.4
Application case.
Only for the microflow control technique overall general picture of itself, famous micro-fluidic expert Mr. Lin Ping Cheng may refer to not
The monograph " diagram Microfluid based Lab on a chip " gone out before long, the monograph is published via Science Press, and the monograph is for miniflow
Past of control technology, now, and, vision of the future etc. etc., suffer from detail, be deep into long opinion of detail
State.
So, the Important Problems of this case that to have a talk below concern.
The basic framework of micro-fluidic chip, including it is etched with the substrate of conduit and the cover plate to fit together therewith, institute
State the fluid course on substrate, before upper cover plate is assembled, it is apparent on see to be exactly some conduits, wait until lid is covered thereon
After piece, just really closure forms the fluid course, and the conduit inner surface of the conduit is together with the part that surround the conduit
Cover plate forms described fluid course together;So, it is clear that assemble the fluid course after completing, its inner surface face
Long-pending major part is the inner surface area of that conduit, and in other words, the state or property of the conduit inner surface are substantially determined
The integrality or property of the fluid course are determined;Therefore say, the inner surface state or interior of this conduit of the structure on substrate
Surface nature is key factor;In principle, any material that can be kept or keep its solid forms substantially, can be used
To make substrate and cover plate, such as, the material that can act as substrate and cover plate can be monocrystalline silicon piece, quartz plate, sheet glass, height
Polymers such as dimethyl silicone polymer, polymethyl methacrylate, makrolon etc.;Certainly, the selection of substrate and the choosing of cover plate
Material be able to can also be differed with identical;From the point of view of material consumption, manufacture difficulty and application popularization prospect etc. etc., these
Not small difference, the especially selection of that substrate between material be present, have a great influence.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, at this
It is extremely simple that conduit is made on the substrate of sample, and the lower cost for material, substrate is made with the polydimethyl siloxane material,
Conduit is being suppressed or etched thereon, and is being engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes,
It is a kind of more satisfactory selection seemingly;Certainly, patch material can also select to use cheap polydimethyl siloxane material:
So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, and making is extremely simple, seems and should also be as
It is extremely easy to popularize, promotes.
But thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that alphabetical PDMS is referred to of abridging, itself are a kind of strong
Strong hydrophobic material, conduit is built on this material, if without being operated for the modified of the conduit surface, then, it is whole
After body assembling is completed, that is, after covering cover plate, because the conduit its inner surface in structure occupies most fluid course
Inner surface, then, its strong hydrophobic property of the PDMS conduits inner surface, is deciding factor, it can be similar to water
The fine liquid stream of polar liquid of solution by becoming very difficult, its flow resistance is big, or even in general Micropump is all difficult to
Promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar;Therefore, existing
Among having technology, modification is modified particular for the conduit inner surface on the PDMS material, is necessary operation;So, this
The individual modified operation for PDMS conduit inner surfaces is pretty troublesomeThat falls nor this problem, forms serious technical puzzlement,
It is another problem:PDMS polymer molecules inside its body phase of this PDMS material substrate have to be diffused to the surface, moves automatically
The characteristic of shifting, the characteristic that this substrate body phase inside PDMS polymer molecules are diffused to the surface, migrated automatically, will cause by table
State after the modification of its inner surface of that conduit of face modifying and decorating can not maintain the sufficiently long time, and that is through surface
Holding time for conduit its inner surface state after modification is substantially only sufficient to the time needs for completing laboratory internal test experiments;
In other words, by surface is modified or the PDMS conduit inner surfaces of surface modification, after it is modified or institute's shape after modification is said
Into surface state can not be lasting, but soon automatically tend to or say become again surface be modified before surface state, compared with
The strong hydrophobic surface state of that script is returned in the short time, then, just think, such micro-fluidic chip can be big
Amount making, mass storage, it is widely popularized, answer is it is obvious that is, impossible.Conduit on this PDMS material, does not do
If surface modification, it can not pump and pass through similar to the fine liquid stream of polar solvent of the aqueous solution, chip also cannot just use;And such as
Fruit has been cooked surface modification, can not persistently keep its state after modifying again, or equally can not popularization and application.
According to the literature, there is a kind of expedient solution, be that a small amount of surface-active is added in sample to be tested solution
Agent, so as to which during test, dynamic, temporary transient effective surface hydrophilic layer is built temporarily in the PDMS conduits inner surface;
However, due to the amphipathic characteristic of surfactant, the introduced surfactant of the program is inevitable simultaneously also can be with sample solution
In tested composition occur combination so that tested composition can not be detected normally or it is detected ratio and dubiously dropped
It is low;The surfactant is possibly even fully wrapped around by tested composition with micelle form so that tested composition can not be detected or
Identification;Therefore, this kind adds the scheme of surfactant into sample solution, is far from a preferable solution.
So, how to accomplish that substrate can either be made using cheap PDMS material, and the conduit can be released
Inner surface decorating state can not persistently, chip can not largely make, largely lay in and then be widely popularized and such a make this area
The puzzlement that numerous professionals are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, it is stated that, its surface is strongly hydrophobic above, this strong hydrophobic
Material surface and also have another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and
And these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into it is gradually deep, until
It is heavy to be trapped within the body phase of PDMS substrates, in fact, this process, partly it is also due to PDMS material body phase interior polymer
Molecule, which has, to be diffused to the surface, caused by travel motion;Such case, it can also be explained from another angle, i.e. continue not
Disconnectedly from inside PDMS body phases to those polymer molecules of its diffusion into the surface, migration, its result moved, be little by little by that
It has been involved in a bit by the large biological molecule of adsorption within the body phase of PDMS substrates, briefly, these adsorbed biologies
Macromolecular is exactly to be swallowed up by PDMS substrate body phases;So, this PDMS substrates body phase swallows up the phenomenon of large biological molecule, its institute
Caused by influence, necessarily cause the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, the problem of PDMS substrates, is, its not only adsorption large biological molecule, and swallow up biological big point
Son, so, as the large biological molecule of experiment test object, its disappearance will not stop because surface saturation is adsorbed, and
It is, it is constantly adsorbed, also constantly swallowed up.
On PDMS substrates, its body phase is constantly swallowed up and tests showing for associated biomolecule macromolecular in related experiment test process
As, it is to say that another kind, which is explained, substantial amounts of Minute pores in PDMS body phases be present, associated biomolecule macromolecular by after adsorption,
Depression enters these Minute pores, and then is swallowed up;However, inventor thinks, those can allow the sky of miniature scale
Qi leel squeezes into the Minute pores therebetween, not equal to saying that they also can directly allow the large biological molecule of relative large scale to enter
Enter, both difference on yardstick are huge, must not make sweeping generalizations.Explanation is bypassed, in any case, as dependence test analysis object
Large biological molecule is adsorbed by PDMS substrate conduits inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective
Existing phenomenon.
, can be from containment PDMS surfaces pair in order to prevent swallow up effect of this PDMS substrate bodies relative to large biological molecule
The absorption of large biological molecule addresses, and method is chemically modified modification aiming at the PDMS material surface, for
For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described channel portion, by chemistry
The conduit inner surface of modification, can contain its absorption to large biological molecule, and then avoid large biological molecule quilt
PDMS substrate body phases are swallowed up;But or that old problem, that is, the chemical modification on PDMS material surface is modified it
Surface state afterwards can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface, migrate automatically
Process, can soon by that by surface chemical modification be modified conduit inner surface state become again script it is strong it is hydrophobic simultaneously
And the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS substrates its
Conduit inner surface is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, and can is enough
Reach and contain the absorption process of the PDMS substrate conduit inner surfaces to large biological molecule for a long time, and then prevent PDMS substrate bodies relative
The effect of swallowing up of large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life,
It is exactly a very intractable problem.The problem equally makes this area numerous specially as another problem addressed above
Industry personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.Should
Also be present many year in problem, so far, also not yet properly settled.
Third, the polydimethyl siloxane material, namely PDMS material, the active force very little wherein between polymer molecule,
The polymer molecule of low polymerization degree inside its body phase is in constantly into the dynamic process of surface migration, therefore, includes PDMS
After it has been formed, the surface topography in its inner-walls of duct face of chip can be because of described low polymerization degree polymer point for the chip of substrate material
Son moves about, pours into the surface and change, and the change of its pattern is somewhat similarly to Rheological Deformation, trickling deformation or creep and become
Pattern caused by shape institute is possible changes;In the higher PDMS material of some low polymerization degree component ratios, this kind somewhat similar
Can be showed in the phenomenon of Rheological Deformation, trickling deformation or the deformation of creep must be than more significant;This kind include PDMS substrates chip its
The dynamic of inner-walls of duct surface topography after shaping changes property, namely the described property or stream that are somewhat similarly to Rheological Deformation
Dropping down the property of deformation or the property of the deformation of creep, the word of rheology one is applied mechanically in this case, integration, also referred to as the rheological equationm of state or rheology become
Shape property, the dynamic of corresponding inner-walls of duct face pattern change, and this case is also referred to as rheology;As described above, because of this kind of material
Expect certain Rheological Deformation property as described above that its own has, its manufactured goods actually can at leisure Rheological Deformation and cause
The manufactured goods total form generation changes;Influence caused by its Rheological Deformation property, it is to be not easy to feel in terms of macroscopic perspective
Examine, still, for the very fine micro-fluidic chip of its internal structure, the rheological equationm of state can but cause bigger shadow
Ring, the fluid course namely the pipeline therefore, during pre-production formed, because the wall of its tube chamber of pipeline is main
It is the wall of PDMS materials, so, the cross-sectional area of the pipeline actually can gradually become because of rheology over time
It is small, stated differently, since the reason for the rheology, the natively finer pipeline that is flowed through for liquid sample
Its tube chamber the more can become the more narrow, in the case of flow time is sufficiently long, or even the pipeline tube chamber can partly close or all
Ground closes, and then make it that sample fluid course is thoroughly blocked, and closes with being even also not reaching to its luminal part of pipeline
The degree closed or fully closed, its fluid passage that can be provided of that pipeline narrow because of rheology, then, in pipeline
Its inner surface originally be exactly strongly it is hydrophobic in the case of, aqueous sample liquid is just more difficult to by becoming more so because of rheology
Narrow micro-channel, then, the influence of substrate rheology how is resisted, maintains the basic framework of the pipeline not occur seriously to become for a long time
The problem of shape, also, keep the long-term unobstructed of the pipeline is exactly a urgent need to resolve.
The content of the invention
The technical problem to be solved by the invention is to provide a package solution, solves totally above
A series of problems addressed, also, the solution is applied to cholera diagnosis multichannel micro-fluidic chip field, form one
Multichannel micro-fluidic device is used in the new cholera diagnosis of kind.
The present invention solves the technical problem by following scheme, and the device that the program provides is that a kind of sample liquid driving is used
Component is capable of the cholera diagnosis micro fluidic device of quick removal, and the structure of the micro fluidic device includes multichannel micro-fluidic core
Piece, the structure of the micro-fluidic chip include being bonded to each other the substrate and cover plate of installing together, and the substrate and cover plate are plate
The conduit knot via mould pressing process or etching technics formation is contained in shape thing or tablet, that face towards the cover plate of the substrate
Structure, the substrate also containing be connected with the channel structure and pierce the substrate via mould pressing process, etching technics or simply beat
The window structure that hole technique is formed, it is bonded to each other the substrate being installed together and has been built into jointly with the cover plate and contain pipeline knot
The micro-fluidic chip of structure and the liquid pool structure being attached thereto, the locations of structures of the pipeline are located at the substrate and mutually pasted with the cover plate
The interface zone of conjunction, its side of the window is blocked by the cover plate and opposite side opens, and the locations of structures of the window is exactly the liquid
The locations of structures in pond, the quantity of the liquid pool is three, and its locations of structures of two liquid pools therein is located at the micro-fluidic chip
Sample introduction end, its locations of structures of a remaining liquid pool are located at liquid flowing in its chip when the actual sample introduction of the micro-fluidic chip is tested
Terminal, the terminal is located remotely from each other with the sample introduction end, and one end of the pipeline is via being similarly positioned in the interface zone being bonded to each other
Manifold shape turnout respectively with the Liang Ge liquid pools UNICOM positioned at sample introduction end, the other end of the pipeline and be located at the micro-fluidic chip
The terminal a remaining liquid pool UNICOM, and, be sequentially respectively installed in the work on diverse location in the pipeline
Electrode and to electrode and reference electrode, the working electrode is by conductive electrode and is attached on the conductive electrode
The gold size sensitive membrane for having embedded cholera specific antigen is formed, and the construction of the pipeline is in parallel construction, and described is in parallel construction
Pipeline is made up of four lateral parallel connections, and the quantity of the working electrode is four, the installation position of four working electrodes
Respectively in four laterals, and, the specificity in its top layer gold size sensitivity membrane structure of four working electrodes
Antigen is the four kinds of cholera antigenic substances that can be specifically bound to cholera antibody respectively, and four kinds of antigenic substances are cholera respectively
TP0821 antigens and cholera TP0319 antigens and cholera TP0624 antigens and cholera O139 antigens, the working electrode its
Material is that sheet or thread is presented in gold material or thermal decomposition conducting polymer material, the working electrode its pattern, and emphasis is,
Its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the table of the primary form
Face its be intended to refer to the not no primary form of the material by any surface chemical modification or any surface chemical modification
Surface, the structure of the device also include chain ring type magnetic holding device, and six links, institute are shared in its structure of the chain ring type magnetic holding device
Link is stated in strip, shaft-like or bar-shaped, each adjacent link is hinged to form chain link, and its appearance profile of the chain link is hexagonal
Cyclic structure, its locations of structures of each drift angle of the hexagonal annular structure is articulated structure position, the hexagonal annular knot
Two therein of structure is magnetic conductivity material in the link of opposite side position its material, four rings of remainder of the hexagonal annular structure
It is non-magnetic material to save its material, and the non magnetic material refers to the material not responded strongly to externally-applied magnetic field, and this is led
Magnet exciting coil is wound with a link among two links of magnetic material, the link for being wound with magnet exciting coil is encouraged at it
The pole shoe of a magnetic conductivity material is respectively equiped with the both sides neighbor positions of magnetic coil, shares two pole shoes, two poles
Boots are directed to the link of another magnetic conductivity material, and fixation is attached on its surface of the link of another magnetic conductivity material and is equiped with
Miniature ultrasonic transducer units, the chain ring type magnetic holding device socket of electromagnetism adhesive strength is relied on to be positioned at its institute of the micro-fluidic chip
State the close position of terminal, and, higher-order of oscillation electric signal transmission cable, one end of the higher-order of oscillation electric signal transmission cable with
The miniature ultrasonic transducer units link together;The chain ring type magnetic holding device provides a function of facilitating the device to disassemble;
Its major function of the miniature ultrasonic transducer units is in the actual sample introduction test of micro-fluidic chip, utilizes the sample introduction end and institute
State on the distance between terminal and the miniature ultrasonic transducer units installation position difference and its ultrasonic intensity experienced
Difference, the difference between its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension, the micro-fluidic core
Interfacial tension difference between the piece both ends can form pressure gap between the both ends of the micro-fluidic chip, the pressure gap
Sample solution can be driven to the end flow;It is soft and have the substrate of the dimethyl silicone polymer material of elasticity its function bag
Include with its property to the strong absorption of ultrasonic wave, ultrasonic wave is absorbed strongly, and thereby in the micro-fluidic chip terminal
The rapid decrement of ultrasonic intensity is realized within to the limited short distance between the sample introduction end;And many silica
Particle, many silica dioxide granules are filled in the pipeline, and its tube chamber of the pipeline is by many silica
Grain is filled up, and for its particle size range of the silica dioxide granule between 10 microns and 200 microns, the silica dioxide granule is dioxy
Inorganic glass of its shared percentage by weight of SiClx crystalline particles, silica glass granule or silica composition more than 80%
Glass particle.
Its particle diameter of the silica dioxide granule can be that the basis between 10 microns and 200 microns is actually needed and any
The particle diameter specified, such as 10 microns, 30 microns, 70 microns, 100 microns, 150 microns or 200 microns of the particle diameter, etc..
The scope of further preferred its particle diameter of the silica dioxide granule is between 100 microns and 200 microns.
Its pattern of the silica dioxide granule is unlimited, and the silica dioxide granule for example can be spheric granules, rod-shaped particles, length
Cube shape particle or any amorphous granular.
The various silica dioxide granules manufacturing technology of itself is known technology.
Various forms, the silica dioxide granule market of all size are on sale.
The silica dioxide granule can also customize to specialized factory.
The preferred scope of its internal diameter of pipeline is between 500 microns and 1000 microns;It is but excellent compared to above-mentioned
The internal diameter of the pipeline scope of choosing, more tiny internal diameter of the pipeline or thicker internal diameter of the pipeline are also what this case was allowed.
The word of magnet exciting coil one art-recognized meanings of itself are known.
The word of magnetic conductivity material one art-recognized meanings of itself are known;The material for belonging to magnetic conductivity material is for example pure
Iron, silicon steel, soft magnetic ferrite etc..
Non magnetic material described in this case refers to the material not responded strongly to externally-applied magnetic field, belongs to the non magnetic material
The material of matter such as metallic copper, metallic zinc, ormolu, metallic aluminium, almag etc..
The be hinged word art-recognized meanings of itself field in Machine Design is known.
The gold size sensitive membrane is to be sufficiently mixed chitosan gold size solution and cholera specific antigen solution uniformly, is used a little
Sample instrument point sample is coated on specified structure position, and forms its drying and forming-film.Cholera specificity in the gold size sensitive membrane
Antigen is the cholera antigen of horseradish peroxidase or glucose oxidase mark, and the gold size sensitive membrane has been included as solid
Determine above-mentioned each cholera specific antigen and introduce complementary medium therein, the complementary medium such as chitosan, acetic acid are fine
Dimension is plain, gelatin is therein a kind of or their mixture.
The pipeline and the lateral and the manifold shape turnout in the microfluidic chip structure, in it
Footpath size may each be arbitrarily selected size, still, for as far as possible less with prepare liquid sample and reduction reagent loss etc.
Consideration, the pipeline and the lateral and the manifold shape turnout preferably select capillary level passage, institute
The passage for stating capillary level implies that its internal diameter passage suitable with the internal diameter of the capillary on ordinary meaning.The capillary is in it
The shape of cross section of portion's passage can be arbitrary shape, and the shape of cross section is for example circular, oval, square, rectangle, bar
Shape, naturally it is also possible to it is the linear of bending arbitrarily be present, also, the interior shape of the capillary is with the extension of pipeline,
The shape of cross section of different parts can also allow to be different shapes.Only for the word of capillary one, its art-recognized meanings is public
Know.
What is be related in structure is the electrode of microsize to electrode and reference electrode, and its electrode shape, which may each be, appoints
The selected shape of meaning, the arbitrarily selected shape such as square piece shape, rectangular patch, strip or round sheet etc..It is described
Art-recognized meanings to electrode and the reference electrode vocabulary of itself are known.
It is related to several liquid pools in this case microfluidic chip structure, the liquid pool is the pond shape or capsule for transitional liquid storage
Shape constructs, and its shape of the inner chamber of each liquid pool may each be arbitrarily selected shape, and the cavity shape is for example round
Cylindrical cavity shape, square column type cavity-like, oblong cavity shape or spherical hollow space shape etc..The size of the liquid pool can be any
Selected size, still, in order to as far as possible less with prepare liquid sample and reduce reagent loss, the liquid pool be preferably capable of with
The liquid pool of the microminiature of capillary matching.
Only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field,
It is known.
Various sizes, variously-shaped ultrasonic transducer are commercially available;Its size of commercially available miniature ultrasonic transducer units
It may diminish to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface its
It is known general technology for the professional in ultrasonic technology field for body.This case is not to this expansion superfluous words.
Only with regard to naked PDMS substrates itself conduit molding or lithographic technique for, be open-and-shut known technology;
Similarly, the technology of hole-opening is even more known simple technique on naked PDMS substrates.This case is not also to this expansion superfluous words.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the micro fluidic device can also include higher-order of oscillation electric signal generator;The higher-order of oscillation electric signal passes
Its other end of transmission cable can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, come for the professional in ultrasonic technology field
Say, be simple and known;The higher-order of oscillation electric signal generator can customize to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 5 milliwatts and 9000 milliwatts
Between;The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with
Between 12MHz.
This case device can further include some annexes certainly, and the annex is such as multiple tracks electrochemical workstation
Deng the art-recognized meanings of the multiple tracks electrochemical workstation are known.The each work being related in this case microfluidic chip structure
Electrode and to electrode and reference electrode etc., special it can get lines crossed and the multiple tracks electrochemical workstation via corresponding respectively
The corresponding interface coupled.It is described that special to get lines crossed be for by each phase of each electrode and the multiple tracks electrochemical workstation
Answer the private cable that interface is coupled to each other.The micro-fluidic chip in this case device, its structure can also include micro-valve,
The quantity of the micro-valve is unlimited, and according to being actually needed, the micro-valve can be installed in any need in the microfluidic chip structure
Position to be mounted;The word of micro-valve one is for the professional of micro fluidic chip technical field, the art-recognized meanings of itself
It is known;The micro-valve itself manufacturing technology and the use of technology is also known;The component that the micro-valve is not required.
The diameter of the working electrode can allow to be that any setting is easily installed the suitable diameter used, still,
It is recommending or say preferable its scope of the diameter between 0.1 micron to 2000 microns;The length of the working electrode can
To allow to be that any setting is easily installed the length used, it is however recommended to or to say the preferable length its scope be 1
Micron is between 15000 microns.
It is installed in by spraying or point sample instrument point sample or the coating of other appropriate process described in the working electrode surface layer
Gold size sensitive membrane, its thicknesses of layers can allow be any setting treat sample measuring liquid occur electrical signals response thickness,
It is however recommended to thickness preferable thickness is between 10 nanometers and 200 nanometers in other words.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, such as:Polypropylene,
Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, for example do
Into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and realized in the extremely short distance to ultrasonic wave
Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip
Using dimethyl silicone polymer it is used as the cover plate, and this case technical scheme is allowed.
Positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal should
Air line distance between a remaining liquid pool can be arbitrarily selected suitable distance, still, the distance as needed
Preferred value is between 3 centimetres and 7 centimetres.
Described its thickness of cover plate and substrate can allow be any setting the thickness for being easy to assembling, the thickness of recommendation or say
Preferable thickness is between 1.0 millimeters and 5.0 millimeters.Less thickness is advantageous to save material.
The application method of this case micro-fluidic chip:
Itd is proposed first based on this case and the first public new liquid stream driving principle, its application of this case micro-fluidic chip are transported
Among work, the new liquid stream driving method is determined completely without involving any additional Micropump.
The interfacial tension difference that this case is formed between micro-fluidic chip both ends caused by the ultrasonic wave,
Driving liquid stream flows in the capillary channel of the four-way micro-fluidic chip, right respectively using four-way electrochemical analytical instrument
Four kinds of cholera antibody are detected.
Corresponding four kinds of cholera antibody is detected with four kinds of cholera antigens, with detecting corresponding four with four kinds of cholera antibody
Kind cholera antigen, similarly, can cholera diagnosis;The principle of its foundation is, necessarily antigen, antibody in cholera patient
And deposit, and mutual Reversible binding forms reversible conjugate, therefore, antibody and thereby cholera diagnosis is detected with antigen, with
Antibody goes to detect antigen and cholera diagnosis, can reach diagnostic purpose.Certainly, its corresponding electrode sensitive of different means is repaiied
Adorn layer technically and differ.
The specific detection of this case micro-fluidic chip is as follows using step:
1st, blood serum sample liquid is added in micro-pipe road, under ultrasonic wave driving, various cholera antibody molecules are by each logical
The cholera specific antigen for the corresponding horseradish peroxidase-labeled that gold size sensitive membrane embeds captures on electrode surface in road.
2nd, the cholera specific antigen of horseradish peroxidase-labeled is formed immune multiple with the cholera antibody in blood serum sample
Compound.
3rd, using multi-channel electrochemical analyzer, the electron mediators such as catechol are added, it is above-mentioned using amperometric detection
Curent change caused by reaction, it is derived from the species and content of various analytes.
4th, result is subjected to comprehensive analysis, comprehensive diagnos is carried out to cholera antibody.
It is an advantage of the invention that its close position relies on electromagnetism adhesive strength set in the terminal of the micro-fluidic chip
The described chain ring type magnetic holding device of positioning is connect, it is miniature super to attach installation in the chain ring type magnetic holding device one magnetic conductivity link
Acoustic wave transducer, with the ultrasonic wave of miniature ultrasonic transducer units transmitting low-power, high-frequency band, meanwhile, utilize poly dimethyl
Its strong absorbability to ultrasonic wave of siloxanes substrate, in shorter distance, it is, from the terminal to it is described enter
In the very short distance of only several centimeters yardsticks between sample end, reach the rapid decrement of ultrasonic intensity, it is thereby micro-fluidic at this
The difference of the interfacial tension is caused at the both ends of chip, and then, utilize its institute of the difference of the interfacial tension between the both ends
Pressure gap between the both ends formed, driving sample liquid stream flow in the pipeline to the terminal direction.By this
Case liquid stream drive scheme, has altogether dispensed with the equipment of traditional Micropump etc;The micro-fluidic chip of Micropump is eliminated, its
Structure is more succinct, and its production process is less, and its cost of manufacture is lower, and this kind of succinct structure is more beneficial for reaching micro-fluidic chip
Low cost application.
Its scheme of this case and by the pipeline except electrode installation take up space in addition to all clearance spaces whole
Filled up with the silica dioxide granule.Those are filled in the silica dioxide granule in pipeline, and this case is to its overall abbreviation titanium dioxide
Silicon grain filler, its function include support, withstand the tube chamber of the pipeline its inwall so that the tube chamber of the pipeline is from because of institute
State rheology and narrow, prevent its reduced cross-sectional area of the tube chamber of the pipeline or even close, so as to maintain the base of the pipeline for a long time
Serious deformation does not occur for this framework.
Numerous silica dioxide granule entities are mutually banked up under this case framework together at random in the pipeline, the titanium dioxide
The function of silicon grain filler, also include certainly with the hydrophilic surface of its silica dioxide granule, utilize each adjacent titanium dioxide
Mutual formed complications are continuous and its inner surface that is mutually close together by water-wetted surface of silicon grain has hydrophilic
The space of property, in the hydrophilic fluid channel of the pipeline Inner Constitution network morphology, although this complications can be continuous
All the time the fluid channel with hydrophilic nmature, then, inside the tube chamber of the pipeline simultaneously and a plurality of miniflow deposited
The effect of its synthesis of road, integrated, cumulative superposition in other words, is the equal of the relatively fine hydrophilic capillary of caliber
Passage;Its presence of the continuous hydrophilic fluid channel of the complications, the PDMS of the primary form is counteracted to a large extent
The flow resistance for liquid sample caused by the strong hydrophobic property in its surface of substrate;In other words, because of liquid sample, it is accounted for
The composition of maximum ratio is water, and liquid sample is substantially exactly the aqueous solution, therefore, the presence of the silica dioxide granule filler,
Can significantly overcome with the unmodified PDMS substrates of hydrophobic property and surface its to the aqueous solution it is incompatible, repel and
Barrier effect, so be greatly reduced belong to properties of Aqueous Solution sample liquid stream its be advanced through the resistance of pipeline.
Under this case framework, inside the tube chamber of the pipeline, the area of its lumen wall wall of former pipeline, because of described two
The rigid of silicon oxide particle filler ties up, the table of part hydrophobic property that still can also be exposed on lumen wall wall
Its area of face is already dramatically reduced, and the area on its still exposed part surface for belonging to hydrophobic property is much
Less than the wall surface area of the hydrophobic property of the lumen wall wall in the presence of no silica dioxide granule filler;
So, under this case framework, in its lumen of the pipeline, its remaining hydrophobic surface increases surface newly with hydrophilic part, and it is folded
Adding, comprehensive, summation, cumulative technique effect, is to form the surface for tending to be hydrophilic on the whole;In other words, in institute
State inside the tube chamber of pipeline, its interfacial tension between sample solution of the part surface of remaining hydrophobic property, plus parent
The part of aqueous nature increases its interfacial tension between sample solution of surface newly, that it is superimposed, comprehensive, summation, cumulative
Technique effect, be so that its solid-liquid interfacial tension inside the tube chamber of the pipeline synergy level off to capillary glass tube its
Interfacial tension between tube chamber inner surface and sample solution.
Therefore, exist in the pipeline under this case framework for having stated silica dioxide granule filler, while rely on this
Case sample liquid stream drive scheme, it becomes possible to the driving to sample liquid stream is reached with relatively low ultrasonic power, with this case mechanism
The test liquid is driven to flow to the end flow.
On the other hand, due to its rheological equationms of state of the PDMS as substrate material, it is filled in the pipeline
The interior silica dioxide granule filler, can gradually by constantly Rheological Deformation and to arest neighbors free space promote PDMS
The pipeline wall of material more tightly wraps up, and this process eventually causes those part two for pressing close to the lumen wall wall
Silicon oxide particle is stuck in original place or is stuck in original place or embedding in-situ silica dioxide granule and its embedded in original place, the part
Incarceration is together, described due to this reason each other for the remaining silica dioxide granule banked up with being mutually closely packed together
Silica dioxide granule filler just will not move in the pipeline easily, silica dioxide granule therein be also substantially all by
Original place is locked in, therefore the stream framework of the micro-fluidic chip can be kept for a long time.
The particle size range of its particle of this case silica dioxide granule filler between 10 microns and 200 microns, compared to
For general organic molecule, general large biological molecule, the particle diameter of its particle of this case silica dioxide granule filler can be rated as huge
Greatly, due to its huge particle diameter, and the inner surface of the surface of its polarity and the pipeline of strong hydrophobic PDMS materials can not
Mutually perhaps say and mutually melt, then, the huge silica dioxide granule of this kind of particle diameter can not be by the pipeline of PDMS materials
Surface is adsorbed, and can not more be swallowed up by the inner surface of the pipeline of PDMS materials, the huge titanium dioxide of this kind of particle diameter
Silicon grain surely not be fully sunk in easily or sink to completely PDMS materials its among intrinsic many Minute pores, and
Because above it is stated that the rheology the reason for, the silica dioxide granule filler can be stuck in original place in other words it is embedding
In original place without moving easily.
Inside the tube chamber of the pipeline, its lumen wall wall of former pipeline is entirely the surface of hydrophobic property originally, such as
Upper described, under this case framework, rigid because of the silica dioxide granule filler ties up, on lumen wall wall still also
Its area of the surface of part hydrophobic property that can be exposed is already dramatically reduced, and it is still exposed to belong to hydrophobicity
The area on the part surface of matter has been far smaller than the tube chamber in the presence of no silica dioxide granule filler
The wall surface area of the hydrophobic property of inwall wall;The reason for because of the rheology, it is close to those dioxies of lumen wall wall
Silicon carbide particle, little by little it is partially embedded into or is partly absorbed among the lumen wall wall, the dioxy among the state
Silicon carbide particle its towards the part surface of lumen wall wall with have occurred and that matching deformation the lumen wall wall it is tight
Closely connected conjunction, then, the matching is deformed and closely pasted with silica particles having occurred and that in lumen wall wall
The part wall of conjunction actually can not contact with flowing through the sample solution of pipeline, that is to say, that the wall of this part is
Through suction-operated can not be produced to the large biological molecule therein that flows through the sample solution of pipeline and organic molecule.Based on above-mentioned
Mechanism, then because fill up the pipeline the silica dioxide granule filler its presence, its lumen wall wall of the pipeline
The overwhelming majority in face is tightly covered, and these described walls tightly covered can not be with flowing through the pipeline
Sample solution contact, only remaining fraction be not brought into close contact by its surface of silica dioxide granule, tightly cover
Hydrophobic surface may be contacted with sample solution, in other words, under this case framework, large biological molecule in sample solution and have
Its direct touch opportunity with hydrophobic PDMS walls of machine molecule is significantly reduced, thus, the lumen wall faces of PDMS materials its
The adsorptive hindrance and interference of swallowing up of large biological molecule and organic molecule are greatly reduced;As described above, this case scheme
Be advantageous to exclude or weaken the adsorptive hindrance and swallow up interference, be advantageous to be lifted the reliability of correlation analysis test data.
As described above, this case silica dioxide granule filler, its function actually includes being similar to being directed to PDMS materials
Inner surface of pipeline carry out chemical modification effect, functioning as its this respect of this case is by the pipeline of the PDMS materials
Surface by the surface modification of hydrophobic property into the surface of hydrophilic nmature, still, with general, usual PDMS surface chemical modifications
Its hydrophilic chemical decorative layer retention time is short, hydrophilic effect can not keep situation prolonged enough different, particle described in this case
Huge silica dioxide granule filler, its particle being stuck by rheology can not both be moved easily, its huge particle diameter
Particle can not more be swallowed up easily by the inner-walls of duct of PDMS materials, and therefore, its from this respect is similar to surface chemical modification
Technique effect on see, its technique effect of this case, be form it is permanent, can not erase, can not consume, not be corroded, not by
Hydrophilic surface reforming layer swallowing up, being not dissolved, it is just comparable to one kind in its correlation table of PDMS base materials in effect
The permanent hydrophilic modifying superficial layer built on face.
It is related that the technical scheme of this case has dissolved its application to dimethyl silicone polymer substrate addressed above totally
A series of technical barriers.Based on this case scheme, the very cheap polydimethyl siloxane material of this kind is just possible to micro- at this
It is prepared by fluidic chip, production, using etc. field play bigger effect.
Attached in the chain ring type magnetic holding device one magnetic conductivity link in this case structure it is fixed installed it is described miniature
Ultrasonic transducer, this architecture provides a function of facilitating the device to disassemble, in this way, the chain ring type magnetic holding device is together with it
The miniature ultrasonic transducer units attached in one magnetic conductivity link just easily can be mutually disengaged with the micro-fluidic chip, that
, the component that the part can freely depart from reuses many times in which just can circulate benignly;The architectural feature is advantageous to save
The use cost of the device.
Brief description of the drawings
Fig. 1 is the schematic diagram of this case device embodiment chip part construction, it is shown that the vertical view of this structure
The structural form of chip internal pipeline and each electrode and each related liquid pool under angle, the chain ring type is not depicted in the figure
Magnetic holding device and the miniature ultrasonic transducer units set up thereon and other annexes.
Fig. 2 be this case device chain ring type magnetic holding device its with appearance side rough under the micro-fluidic chip socket state
View, the legend and indicates the relative position relation under the socket state between each component.
Fig. 3 is the outside side view under another viewing angle of this case device;Fig. 3 viewing angle and Fig. 2's
Viewing angle is mutually perpendicular to.
In figure, 1,2,10 be the different liquid pool of three installation positions respectively, and 3 be manifold shape turnout, and 4,7,11,14 are respectively
Installation position is different but four laterals parallel with one another for forming UNICOM's structure in parallel, and 5 be its that be installed in lateral 4
Specific antigenic substance in the gold size sensitivity membrane structure of top layer is the working electrode of cholera TP0821 antigens, and 6 be to be installed in branch
The specific antigenic substance in its top layer gold size sensitivity membrane structure in pipeline 7 is the working electrode of cholera TP0319 antigens, 12
It is that specific antigenic substance in its top layer gold size sensitivity membrane structure being installed in lateral 11 is cholera TP0624 antigens
Working electrode, 13 be specific antigenic substance in its top layer gold size sensitivity membrane structure being installed in lateral 14 for suddenly
The working electrode of random O139 antigens, 8 be to electrode, and 9 be reference electrode, and 15 be chain ring type magnetic holding device, and 16 be the micro-fluidic core
The terminal of piece, 17 be the sample introduction end of the micro-fluidic chip, and 18 be the substrate of dimethyl silicone polymer material, and 19 be lid
Piece, 20 be miniature ultrasonic transducer units, and 21 be higher-order of oscillation electric signal transmission cable, and 22,29 be to be located at different structure position respectively
The link for the two magnetic conductivity materials put, the links of two magnetic conductivity materials end-to-end relation each other in the structure shown here, 23,32 points
It is not two pole shoes positioned at different structure position, 24,26,28,31,34,36 be six in six diverse locations respectively
Pin joint, 25,27,33,35 be the link of four non magnetic materials in four diverse locations respectively, and 30 be magnet exciting coil;
The chain ring type magnetic holding device structure in legend is only the legend structure of signal, and the actual chain ring type magnetic holding device structure is not limited to
The legend chain ring type magnetic holding device structure;Arrow in legend indicate the micro-fluidic chip its in actual motion, by two
End pressure difference driving, the flow direction of its sample liquid stream.
Embodiment
In this case that Fig. 1, Fig. 2 and Fig. 3 are shown embodiment, the structure of the micro fluidic device includes multichannel miniflow
Chip is controlled, the structure of the micro-fluidic chip includes being bonded to each other the substrate 18 and cover plate 19 of installing together, the He of substrate 18
Cover plate 19 is plate object or tablet, and that face towards the cover plate 19 of the substrate 18 is contained via mould pressing process or etching
Technique formed channel structure, the substrate 18 also containing be connected with the channel structure and pierce the substrate via stamper
The window structure that skill, etching technics or simple drilling technology are formed, it is bonded to each other the substrate 18 and the cover plate being installed together
19 have been built into the micro-fluidic chip containing pipeline configuration and the liquid pool structure being attached thereto jointly, the liquid pool be liquid pool 1,
Liquid pool 2, liquid pool 10, the locations of structures of the pipeline are located at the interface zone that the substrate 18 is bonded to each other with the cover plate 19, the window
Its side is blocked by the cover plate 19 and opposite side opens, and the locations of structures of the window is exactly the liquid pool 1, liquid pool 2, liquid pool 10
Locations of structures, the quantity of the liquid pool is three, two liquid pools therein be liquid pool 1 and liquid pool 2 its locations of structures to be located at this micro-
The sample introduction end 17 of fluidic chip, a remaining liquid pool are that liquid pool 10 its locations of structures is located at the actual sample introduction of the micro-fluidic chip and surveyed
The terminal 16 that liquid flows in its chip during examination, the terminal 16 are located remotely from each other with the sample introduction end 17, and one end of the pipeline is via same
Sample be located at the manifold shape turnout 3 of the interface zone being bonded to each other respectively with liquid pool 1 and the liquid pool 2 positioned at sample introduction end 17
Logical, the other end of the pipeline and a remaining liquid pool for the terminal 16 positioned at the micro-fluidic chip are liquid pool 10
It is logical, and, the working electrode that is sequentially respectively installed in the pipeline on diverse location and to electrode 8 and reference electrode 9, institute
It is quick by conductive electrode and the gold size for having embedded cholera specific antigen being attached on the conductive electrode to state working electrode
Feel film to form, the construction of the pipeline is in parallel construction, and the pipeline in parallel construction is made up of four lateral parallel connections, institute
The quantity for stating working electrode is four, and the installation position of four working electrodes in four laterals, is somebody's turn to do respectively
Four working electrodes are working electrode 5, working electrode 6, working electrode 12, working electrode 13 respectively, and, this four work electricity
Specific antigen extremely in top layer gold size sensitivity membrane structure is that the four kinds of cholera that can be specifically bound to cholera antibody resist respectively
Original matter, four kinds of antigenic substances are cholera TP0821 antigens and cholera TP0319 antigens and cholera TP0624 antigens respectively
And cholera O139 antigens, for specifically deploying, 5 be in its top layer gold size sensitivity membrane structure being installed in lateral 4
Specific antigenic substance be cholera TP0821 antigens working electrode, 6 be that its top layer gold size being installed in lateral 7 is quick
Feel the working electrode that the specific antigenic substance in membrane structure is cholera TP0319 antigens, 12 are installed in lateral 11
Specific antigenic substance in its top layer gold size sensitivity membrane structure is the working electrode of cholera TP0624 antigens, and 13 be to be installed in point
The specific antigenic substance in its top layer gold size sensitivity membrane structure in branch pipe(tube) 14 is the working electrode of cholera O139 antigens, institute
It is gold material or thermal decomposition conducting polymer to state working electrode 5, working electrode 6, working electrode 12, working electrode 13 its material
Material, the working electrode 5, working electrode 6, working electrode 12, its pattern presentation sheet or thread of working electrode 13, emphasis
It is that its material of the substrate 18 is dimethyl silicone polymer material, its surface of substrate 18 is the surface of primary form, the primary shape
The surface of state its be intended to refer to the primary of the not no material by any surface chemical modification or any surface chemical modification
The surface of form, the structure of the device also include chain ring type magnetic holding device 15, are shared in its structure of the chain ring type magnetic holding device 15
Six links 22,25,27,29,33,35, the link 22,25,27,29,33,35 are each adjacent in strip, shaft-like or bar-shaped
Link is hinged to form chain link, the hexagonal cyclic structure of its appearance profile of the chain link, the hexagonal annular structure it is each
Drift angle is that drift angle 24,26,28,31,34,36 its locations of structures is articulated structure position, and the hexagonal annular structure is therein
Two links in opposite side position are that link 22,29 its material is magnetic conductivity material, the remainder four of the hexagonal annular structure
Individual link be link 25,27,33,35, its material be non-magnetic material, the non magnetic material refers to not having to externally-applied magnetic field
There is the material responded strongly, two links of the magnetic conductivity material are that a link among link 22,29 twines in link 29
Magnet exciting coil 30 is wound with, the link 29 for being wound with magnet exciting coil 30 is respectively installed in the both sides neighbor positions of its magnet exciting coil 30
There is the pole shoe of a magnetic conductivity material, share two pole shoes, the pole shoe is pole shoe 23 and pole shoe 32, and two pole shoes are
Pole shoe 23 and pole shoe 32 are directed to the link 22 of another magnetic conductivity material, its surface of the link 22 of another magnetic conductivity material
Upper attaching is fixed to be equiped with miniature ultrasonic transducer units 20, relies on the chain ring type magnetic holding device 15 socket of electromagnetism adhesive strength fixed
The close position of position its terminal 16 in the micro-fluidic chip, and, higher-order of oscillation electric signal transmission cable 21, the high frequency vibrating
The one end and the miniature ultrasonic transducer units 20 for swinging electric signal transmission cable 21 link together;The chain ring type magnetic holding device 15 carries
A function of facilitating the device to disassemble is supplied;Its major function of the miniature ultrasonic transducer units 20 is actual in micro-fluidic chip
When sample introduction is tested, using between the sample introduction end 17 and the terminal 16 and the installation position of miniature ultrasonic transducer units 20
Difference in distance difference and its ultrasonic intensity experienced, its interfacial tension of sample introduction end 17 and institute described in induced synthesis
The difference between terminal 16 its interfacial tension is stated, the micro-fluidic chip both ends are the interface between sample introduction end 17 and terminal 16
Power difference can be to form pressure gap between sample introduction end 17 and terminal 16 at the both ends of the micro-fluidic chip, the pressure gap meeting
Driving sample solution flows to the direction of terminal 16;The substrate 18 of the dimethyl silicone polymer material that is soft and having elasticity its
Function is also included with its property to the strong absorption of ultrasonic wave, and ultrasonic wave is absorbed strongly, and thereby in the micro-fluidic core
The piece terminal 16 is to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end 17;And permitted
More silica dioxide granules, many silica dioxide granules are filled in the pipeline, and its tube chamber of the pipeline is by many
Silica dioxide granule filled up, its particle size range of the silica dioxide granule is between 10 microns and 200 microns, the titanium dioxide
Silicon grain is silica crystalline particles, silica glass granule or silica composition its shared percentage by weight 80%
Inorganic glass particles above.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its try
The flow direction of sample liquid stream.
The chain ring type magnetic holding device 15 is depicted without in Fig. 1 and attaches fixation in one magnetic conductivity material link
Miniature ultrasonic transducer units 20 and higher-order of oscillation electric signal transmission cable 21 of installing;Also, in Fig. 1, Fig. 2 and Fig. 3
It is depicted without the associate members such as the higher-order of oscillation electric signal generator and multiple tracks electrochemical workstation.
Involved magnetic conductive material and involved nonmagnetic substance market are on sale.
Involved miniature ultrasonic transducer units 20 are commercially available;It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal transmission cable 21 is commercially available;Can also be special to ultrasonic transducer producer or cable
Industry producer customizes.
Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available;It can also determine to relevant manufacturers
System.
Each working electrode in this example structure and can be respectively via each special electricity to electrode and reference electrode
Cable or say is being got lines crossed respectively with the corresponding cable interface of the multiple tracks electrochemical workstation as annex or saying interface connection of getting lines crossed.
This case device is that a variety of corresponding cholera antibody are detected with a variety of cholera antigens, thereby cholera diagnosis.
Claims (10)
1. sample liquid driving component is capable of the cholera diagnosis micro fluidic device of quick removal, the structure bag of the micro fluidic device
Multichannel micro-fluidic chip is included, the structure of the micro-fluidic chip includes being bonded to each other the substrate and cover plate of installing together, described
Substrate and cover plate are plate object or tablet, and that face towards the cover plate of the substrate is contained via mould pressing process or etching
Technique formed channel structure, the substrate also containing be connected with the channel structure and pierce the substrate via mould pressing process,
The window structure that etching technics or simple drilling technology are formed, it is bonded to each other the substrate and the common structure of the cover plate being installed together
The micro-fluidic chip containing pipeline configuration and the liquid pool structure being attached thereto is built up, the locations of structures of the pipeline is located at the base
The interface zone that piece is bonded to each other with the cover plate, its side of the window is blocked by the cover plate and opposite side opens, the knot of the window
Structure position is exactly the locations of structures of the liquid pool, and the quantity of the liquid pool is three, its structure bit set of two liquid pools therein
In the sample introduction end of the micro-fluidic chip, when its locations of structures of a remaining liquid pool is located at the test of the micro-fluidic chip actual sample introduction
The terminal that liquid flows in its chip, the terminal is located remotely from each other with the sample introduction end, and one end of the pipeline is via being similarly positioned in the phase
The manifold shape turnout for the interface zone being mutually bonded respectively with the Liang Ge liquid pools UNICOM positioned at sample introduction end, the other end of the pipeline with
Positioned at a remaining liquid pool UNICOM for the terminal of the micro-fluidic chip, and, sequentially it is respectively installed in the pipeline
Working electrode on diverse location and to electrode and reference electrode, the working electrode is by conductive electrode and is attached to
The gold size sensitive membrane for having embedded cholera specific antibody on the conductive electrode is formed, and the construction of the pipeline is in parallel construction,
The pipeline in parallel construction is made up of four lateral parallel connections, and the quantity of the working electrode is four, four works
The installation position for making electrode is located in four laterals respectively, and, its top layer gold size of four working electrodes is sensitive
Specific antigen in membrane structure is the four kinds of cholera antigenic substances that can be specifically bound to cholera antibody respectively, four kinds of antigens
Material is cholera TP0821 antigens and cholera TP0319 antigens and cholera TP0624 antigens and cholera O139 antigens respectively,
Its material of the working electrode is gold material or thermal decomposition conducting polymer material, and sheet is presented in its pattern of the working electrode
It is or thread, it is characterised in that its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is the table of primary form
Face, the surface of the primary form its be intended to refer to not by any surface chemical modification or any surface chemical modification should
The surface of the primary form of material, the structure of the device also include chain ring type magnetic holding device, its structure of the chain ring type magnetic holding device
In share six links, the link is in strip, shaft-like or bar-shaped, and each adjacent link is hinged to form chain link, the chain link
The hexagonal cyclic structure of its appearance profile, its locations of structures of each drift angle of the hexagonal annular structure is articulated structure position
Put, two therein of the hexagonal annular structure is magnetic conductivity material in the link of opposite side position its material, the hexagonal rings
Its material of the remaining four processes of shape structure is non-magnetic material, and the non magnetic material refers to not strong to externally-applied magnetic field
The material of strong response, magnet exciting coil is wound with a link among two links of the magnetic conductivity material, this, which is wound with, encourages
The link of magnetic coil is respectively equiped with the pole shoe of a magnetic conductivity material in the both sides neighbor positions of its magnet exciting coil, shares two
The pole shoe, two pole shoes are directed to the link of another magnetic conductivity material, its table of the link of another magnetic conductivity material
The fixed chain ring type magnetic holding device socket positioning for being equiped with miniature ultrasonic transducer units, relying on electromagnetism adhesive strength is attached on face
In the close position of its terminal of the micro-fluidic chip, and, higher-order of oscillation electric signal transmission cable, the higher-order of oscillation telecommunication
One end of number transmission cable links together with the miniature ultrasonic transducer units;The chain ring type magnetic holding device provides a convenience
The function that the device is disassembled;Its major function of the miniature ultrasonic transducer units is the profit in micro-fluidic chip actual sample introduction test
With the distance between the sample introduction end and the terminal and miniature ultrasonic transducer units installation position difference and its felt
Between the difference on ultrasonic intensity being subject to, its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension
Difference, the interfacial tension difference between the micro-fluidic chip both ends can form pressure between the both ends of the micro-fluidic chip
Power difference, the pressure gap can drive sample solution to the end flow;The dimethyl silicone polymer that is soft and having elasticity
Its function of the substrate of material is included with its property to the strong absorption of ultrasonic wave, and ultrasonic wave is absorbed strongly, and thereby exists
The micro-fluidic chip terminal is to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end;With
And many silica dioxide granules, many silica dioxide granules are filled in the pipeline, its tube chamber of the pipeline is by this
Many silica dioxide granules are filled up, and its particle size range of the silica dioxide granule is between 10 microns and 200 microns, and this two
Silicon oxide particle is that silica crystalline particles, silica glass granule or silica composition its shared percentage by weight exists
More than 80% inorganic glass particles.
2. sample liquid driving component according to claim 1 is capable of the cholera diagnosis micro fluidic device of quick removal,
Characterized in that, the pipeline and the lateral and the manifold shape turnout are capillary channel.
3. sample liquid driving component according to claim 1 is capable of the cholera diagnosis micro fluidic device of quick removal,
Characterized in that, the thermal decomposition conducting polymer is the conduction formed by polyimides or polyacrylonitrile after anoxybiotic is heat-treated
Property material.
4. sample liquid driving component according to claim 1 is capable of the cholera diagnosis micro fluidic device of quick removal,
Characterized in that, the width or diameter of the working electrode be between 0.1 micron to 2000 microns, and, the work electricity
The length of pole is between 1 micron to 15000 microns.
5. sample liquid driving component according to claim 1 is capable of the cholera diagnosis micro fluidic device of quick removal,
Characterized in that, the thickness of the gold size sensitive membrane is between 10 nanometers and 200 nanometers.
6. sample liquid driving component according to claim 1 is capable of the cholera diagnosis micro fluidic device of quick removal,
Characterized in that, the cover plate its material in structure is dimethyl silicone polymer material.
7. sample liquid driving component according to claim 1 is capable of the cholera diagnosis micro fluidic device of quick removal,
Characterized in that, positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal
Air line distance between a remaining liquid pool is between 3 centimetres and 7 centimetres;It is thick for the cover plate and substrate in structure
Degree is between 1.0 millimeters and 5.0 millimeters.
8. sample liquid driving component according to claim 1 is capable of the cholera diagnosis micro fluidic device of quick removal,
Characterized in that, its particle size range of the silica dioxide granule is between 100 microns and 200 microns, its internal diameter model of the pipeline
Enclose between 500 microns and 1000 microns.
9. sample liquid driving component according to claim 1 is capable of the cholera diagnosis micro fluidic device of quick removal,
Characterized in that, the structure of the micro fluidic device also includes higher-order of oscillation electric signal generator, the higher-order of oscillation electric signal passes
Its other end of transmission cable is connected with the higher-order of oscillation electric signal generator.
10. sample liquid driving component according to claim 1 is capable of the cholera diagnosis micro fluidic device of quick removal,
Characterized in that, its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units, between 5 milliwatts and 9000 milliwatts, this is micro-
The frequency of its ultrasonic wave operationally launched of type ultrasonic transducer is between 100KHz and 12MHz.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610356871.6A CN107362842A (en) | 2016-05-13 | 2016-05-13 | The cholera diagnosis micro fluidic device of quick removal is capable of in sample liquid driving with component |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610356871.6A CN107362842A (en) | 2016-05-13 | 2016-05-13 | The cholera diagnosis micro fluidic device of quick removal is capable of in sample liquid driving with component |
Publications (1)
Publication Number | Publication Date |
---|---|
CN107362842A true CN107362842A (en) | 2017-11-21 |
Family
ID=60304723
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610356871.6A Withdrawn CN107362842A (en) | 2016-05-13 | 2016-05-13 | The cholera diagnosis micro fluidic device of quick removal is capable of in sample liquid driving with component |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107362842A (en) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
CN102645429A (en) * | 2012-04-23 | 2012-08-22 | 宁波大学 | Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device |
CN103285949A (en) * | 2013-05-27 | 2013-09-11 | 苏州扬清芯片科技有限公司 | Micro-fluidic serum extracting chip |
-
2016
- 2016-05-13 CN CN201610356871.6A patent/CN107362842A/en not_active Withdrawn
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
CN102645429A (en) * | 2012-04-23 | 2012-08-22 | 宁波大学 | Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device |
CN103285949A (en) * | 2013-05-27 | 2013-09-11 | 苏州扬清芯片科技有限公司 | Micro-fluidic serum extracting chip |
Non-Patent Citations (1)
Title |
---|
JAMES FRIEND等: "Microscale acoustofluidics: microfluidics driven via acoustics and ultrasonics", 《REVIEWS OF MODERN PHYSICS》 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107754915A (en) | Interfacial characteristics is relied on to carry out the cheap micro fluidic device of cholera diagnosis of liquid stream driving | |
CN107362839A (en) | Additional liquid sample driving is with the easily-disassembled AIDS diagnosis device of building blocks of function | |
CN107754878A (en) | Using the controller used in syphilis diagnosis of interfacial characteristics transmission liquid stream with cheap micro fluidic device | |
CN107362842A (en) | The cholera diagnosis micro fluidic device of quick removal is capable of in sample liquid driving with component | |
CN107754901A (en) | The cholera diagnosis micro fluidic device of quick removal is capable of in sample liquid driving with component | |
CN107537594A (en) | Easily-disassembled hypotype swine flu multi-channel testing device comprising electromagnetism auxiliary part | |
CN107754894A (en) | The controller used in syphilis diagnosis device that sample driving can be removed fast with function element | |
CN107754900A (en) | The readily removable controller used in syphilis diagnosis micro fluidic device removed of liquid stream driving building blocks of function | |
CN107754875A (en) | It is a kind of to be not only easily installed but also be easy to the cholera diagnosis Multichannel device disassembled | |
CN107362836A (en) | It is a kind of to be not only easily installed but also be easy to the cholera diagnosis Multichannel device disassembled | |
CN107754871A (en) | Be easy to disassemble applies the hypotype swine flu detection device of cheap material | |
CN107754905A (en) | The driving of sample liquid is easy to the AIDS diagnosis device of removal with component | |
CN107362837A (en) | Be easy to disassemble applies the hypotype swine flu detection device of cheap material | |
CN107754897A (en) | Additional liquid sample driving is with the easily-disassembled AIDS diagnosis device of building blocks of function | |
CN107537596A (en) | The cholera diagnosis micro fluidic device of quick removal is capable of in sample liquid driving with component | |
CN107754879A (en) | The special cheap cholera diagnosis micro fluidic device of sap flow process mode | |
CN107362841A (en) | Easily-disassembled hypotype swine flu multi-channel testing device comprising electromagnetism auxiliary part | |
CN107754881A (en) | Easily-disassembled hypotype swine flu multi-channel testing device comprising electromagnetism auxiliary part | |
CN107537593A (en) | The readily removable controller used in syphilis diagnosis micro fluidic device removed of liquid stream driving building blocks of function | |
CN107349975A (en) | The easily-disassembled cholera diagnosis device of liquid stream driving is carried out by means of interfacial characteristics | |
CN107345963A (en) | Using the controller used in syphilis diagnosis of interfacial characteristics transmission liquid stream with cheap micro fluidic device | |
CN107345960A (en) | Interfacial characteristics is relied on to carry out the cheap micro fluidic device of cholera diagnosis of liquid stream driving | |
CN107754911A (en) | The easily-disassembled cholera diagnosis device of liquid stream driving is carried out by means of interfacial characteristics | |
CN107349977A (en) | The cholera diagnosis micro fluidic device that can be fast removed for the annex for driving liquid stream | |
CN107349976A (en) | The controller used in syphilis diagnosis device that a kind of sample driving can be removed fast with adapter |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WW01 | Invention patent application withdrawn after publication | ||
WW01 | Invention patent application withdrawn after publication |
Application publication date: 20171121 |