CN107649219A - Easily-disassembled hypotype swine flu multi-channel testing device comprising electromagnetism auxiliary part - Google Patents
Easily-disassembled hypotype swine flu multi-channel testing device comprising electromagnetism auxiliary part Download PDFInfo
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- CN107649219A CN107649219A CN201610628385.5A CN201610628385A CN107649219A CN 107649219 A CN107649219 A CN 107649219A CN 201610628385 A CN201610628385 A CN 201610628385A CN 107649219 A CN107649219 A CN 107649219A
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0609—Holders integrated in container to position an object
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
- B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
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Abstract
The present invention relates to a kind of easily-disassembled hypotype swine flu multi-channel testing device for including electromagnetism auxiliary part, belong to analysis testing field.The substrate physical presence series problem of hypotype swine flu diagnosis micro-fluidic chip is made using dimethyl silicone polymer i.e. PDMS that is cheap and easily processing；Its surface of the PDMS material is strongly hydrophobic, and targetedly surface is modified or surface modification, its effect are difficult to persistently, and this case aims to solve the problem that the serial relevant issues.This case main points are, the selected PDMS with ecosystem surface of substrate, and the chain ring type magnetic holding device for being equiped with miniature ultrasonic transducer units socket is positioned at the sample liquid stream terminal of the micro-fluidic chip its neighbor positions, interfacial tension is reduced with ultrasonic wave, strong absorbability using PDMS to ultrasonic wave simultaneously, reach ultrasonic intensity rapid decrement in short distance, so as to form interfacial tension difference at the both ends of the chip, thereby facilitate sample liquid stream to be flowed along capillary channel to the terminal direction.
Description
Technical field
The present invention relates to a kind of easily-disassembled hypotype swine flu multi-channel testing device for including electromagnetism auxiliary part, the dress
It is to diagnose the special purpose device of hypotype swine flu antigen based on antigen/antibody specific reaction to put, and belongs to analysis testing field.
Background technology
Multichannel micro-fluidic hypotype swine flu diagnostic techniques background, it is special to may refer to the grade inventions of CN 201110311127.1
Sharp application case.
Only for the microflow control technique overall general picture of itself, famous micro-fluidic expert Mr. Lin Ping Cheng may refer to not
The monograph " diagram Microfluid based Lab on a chip " gone out before long, the monograph is published via Science Press, and the monograph is for miniflow
Past of control technology, now, and, vision of the future etc. etc., suffer from detail, be deep into long opinion of detail
State.
So, the Important Problems of this case that to have a talk below concern.
The basic framework of micro-fluidic chip, including be etched with the substrate of small fluid course and fit together therewith
Cover plate, the small fluid course on the substrate, before upper cover plate is assembled, it is apparent on see to be exactly some micro-channels, to wait until
After covering cover plate thereon, just really closure forms the small fluid course, the conduit inner surface of the micro-channel together with
The part cover plate that surround the micro-channel forms described small fluid course together；So, it is clear that after assembling completes
The small fluid course, the major part of its inner surface area is the inner surface area of that micro-channel, in other words, should
The state or property of micro-channel inner surface substantially determine the integrality or property of the small fluid course；Therefore say, this
The inner surface state or inner surface property of micro-channel of the individual structure on substrate are key factors；In principle, it is any can
The material of its solid forms is kept or kept substantially, can be used to make substrate and cover plate, such as, it can act as substrate and lid
The material of piece can be monocrystalline silicon piece, quartz plate, sheet glass, high polymer for example dimethyl silicone polymer, polymethyl methacrylate,
Makrolon etc.；Certainly, the selection of substrate and the selection of cover plate be able to can also be differed with identical；From material consumption, make
From the point of view of difficulty and application popularization prospect etc. etc., not small difference, the especially choosing of that substrate between these materials be present
Material, have a great influence.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, at this
It is extremely simple that micro-channel is made on the substrate of sample, and the lower cost for material, base is made with the polydimethyl siloxane material
Piece, micro-channel, and the cover plate phase made with the cheap material such as glass or polypropylene or other plastic sheets are being suppressed or etched thereon
Coordinate, be a kind of more satisfactory selection seemingly；Certainly, patch material can also select to use cheap dimethyl silicone polymer
Material：So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, and making is extremely simple, seems
It should be extremely easy to popularize, promote.
But thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that alphabetical PDMS is referred to of abridging, itself are a kind of strong
Strong hydrophobic material, micro-channel is built on this material, if without being operated for the modified of the micro-channel surface, that
, after overall assembling is completed, that is, after covering cover plate, because the micro-channel its inner surface in structure occupies most liquid
The inner surface of circulation road, then, its strong hydrophobic property of the PDMS micro-channels inner surface, is deciding factor, it can cause
Similar to the aqueous solution the fine liquid stream of polar liquid by becoming very difficult, its flow resistance is big, or even in general is micro-
Pump is all difficult to promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar；
Therefore, among prior art, modification is modified particular for the micro-channel inner surface on the PDMS material, is necessary behaviour
Make；So, this is pretty troublesome for the modified operation of PDMS micro-channel inner surfacesThat falls nor this problem, is formed tight
Weight technology puzzlement, be another problem：PDMS polymer molecules inside its body phase of this PDMS material substrate have automatic
The characteristic diffuse to the surface, migrated, the spy that this substrate body phase inside PDMS polymer molecules are diffused to the surface, migrated automatically
Property, the state after modification by its inner surface of that micro-channel of surface modifying and decorating will be caused can not to maintain long enough
Time, the holding time for micro-channel its inner surface state after that is surface-modified be substantially only sufficient to complete laboratory internal
The time of test experiments needs；In other words, by surface modification or the PDMS micro-channel inner surfaces of surface modification, it is modified
The surface state that is formed can not be lasting afterwards or after saying modification, but soon automatically tends to or say and become surface modification again
Surface state before, the strong hydrophobic surface state of that script is returned in the shorter time, then, just think, this
The micro-fluidic chip of sample can largely make, mass storage, be widely popularized, and answer is it is obvious that is, impossible.This
Micro-channel on PDMS material, if not doing surface modification, similar to the aqueous solution the fine liquid stream of polar solvent can not pump it is logical
Cross, chip also cannot just use；And if having done surface modification, its state after modifying can not be persistently kept again, or together
Sample can not popularization and application.
So, how to accomplish that substrate can either be made using cheap PDMS material, and the microflute can be released
Road inner surface decorating state can not persistently, chip can not largely make, largely lay in and then be widely popularized and such a make ability
The puzzlement that the numerous professionals in domain are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, it is stated that, its surface is strongly hydrophobic above, this strong hydrophobic
Material surface and also have another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and
And these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into it is gradually deep, until
It is heavy to be trapped within the body phase of PDMS substrates, in fact, this process, partly it is also due to PDMS material body phase interior polymer
Molecule, which has, to be diffused to the surface, caused by travel motion；Such case, it can also be explained from another angle, i.e. continue not
Disconnectedly from inside PDMS body phases to those polymer molecules of its diffusion into the surface, migration, its result moved, be little by little by that
It has been involved in a bit by the large biological molecule of adsorption within the body phase of PDMS substrates, briefly, these adsorbed biologies
Macromolecular is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up the phenomenon of large biological molecule, its institute
Caused by influence, necessarily cause the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, the problem of PDMS substrates, is, its not only adsorption large biological molecule, and swallow up biological big point
Son, so, as the large biological molecule of experiment test object, its disappearance will not stop because surface saturation is adsorbed, and
It is, it is constantly adsorbed, also constantly swallowed up.
On PDMS substrates, its body phase is constantly swallowed up and tests showing for associated biomolecule macromolecular in related experiment test process
As, it is to say that another kind, which is explained, substantial amounts of Minute pores in PDMS body phases be present, associated biomolecule macromolecular by after adsorption,
Depression enters these Minute pores, and then is swallowed up；However, inventor thinks, those can allow the sky of miniature scale
Qi leel squeezes into the Minute pores therebetween, not equal to saying that they also can directly allow the large biological molecule of relative large scale to enter
Enter, both difference on yardstick are huge, must not make sweeping generalizations.Explanation is bypassed, in any case, as dependence test analysis object
Large biological molecule is adsorbed by PDMS substrate micro-channels inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is person in charge of reception at ceremonies
Phenomenon existing for sight.
, can be from containment PDMS surfaces pair in order to prevent swallow up effect of this PDMS substrate bodies relative to large biological molecule
The absorption of large biological molecule addresses, and method is chemically modified modification aiming at the PDMS material surface, for
For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described micro-channel part, by changing
The micro-channel inner surface of modification is learned, its absorption to large biological molecule can be contained, and then avoid large biological molecule
Swallowed up by PDMS substrate body phases；But or that old problem, that is, the chemical modification on PDMS material surface is modified
Surface state afterwards can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface, move automatically
The process of shifting, it soon can become that micro-channel inner surface state being modified by surface chemical modification again script strong and dredge
Water and the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS bases
Its micro-channel inner surface of piece is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, and can is enough
Reach and contain the absorption process of the PDMS substrate micro-channel inner surfaces to large biological molecule for a long time, and then prevent PDMS substrate body phases
The effect of swallowing up to large biological molecule so that related chip manufactured goods be able to maintain that one it is prolonged enough, reasonably guarantee the quality
Phase, it is exactly a very intractable problem.The problem equally makes this area numerous as another problem addressed above
Professional is entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.
Also be present many year in the problem, so far, also not yet properly settled.
The content of the invention
The technical problem to be solved by the invention is to provide a package solution, solves totally above
Two problems addressed, also, the solution is applied to hypotype swine flu diagnosis multichannel micro-fluidic chip field, shape
Into a kind of new hypotype swine flu diagnosis Multichannel device.
The present invention solves the technical problem by following scheme, and the device that the program provides is that a kind of electromagnetism that includes aids in
The easily-disassembled hypotype swine flu multi-channel testing device of component, the structure of the device include multichannel micro-fluidic chip, and this is micro-
The structure of fluidic chip includes being bonded to each other the substrate and cover plate of installing together, and the substrate and cover plate are plate object or piece
The channel structure via mould pressing process or etching technics formation, the base are contained in shape thing, that face towards the cover plate of the substrate
Piece also containing be connected with the channel structure and pierce the substrate via mould pressing process, etching technics or simple drilling technology shape
Into window structure, be bonded to each other the substrate that is installed together and the cover plate be built into jointly containing pipeline configuration and with
Connected liquid pool structure micro-fluidic chip, the locations of structures of the pipeline be located at the interface that the substrate and the cover plate are bonded to each other
Region, its side of the window is blocked by the cover plate and opposite side opens, and the locations of structures of the window is exactly the structure of the liquid pool
Position, the quantity of the liquid pool is three, and its locations of structures of two liquid pools therein is located at the sample introduction end of the micro-fluidic chip, remaining
Under the liquid flowing in its chip when being located at the actual sample introduction test of the micro-fluidic chip of liquid pool its locations of structures terminal, should
Terminal is located remotely from each other with the sample introduction end, and one end of the pipeline is branched off via the manifold shape for being similarly positioned in the interface zone being bonded to each other
Road respectively with the Liang Ge liquid pools UNICOM positioned at sample introduction end, the other end of the pipeline with positioned at the micro-fluidic chip the terminal
A remaining liquid pool UNICOM, and, the working electrode that is sequentially respectively installed in the pipeline on diverse location and right
Electrode and reference electrode, the working electrode is by conductive electrode and is attached on the conductive electrode and has embedded hypotype
The gold size sensitive membrane of swine flu specific antibody is formed, and the construction of the pipeline is in parallel construction, the pipeline in parallel construction
It is made up of three lateral parallel connections, the quantity of the working electrode is three, the installation position difference of three working electrodes
In three laterals, and, the specific antibody in its top layer gold size sensitivity membrane structure of three working electrodes
It is the three kinds of hypotype swine flu antibody materials that can be specifically bound to hypotype swine flu antigen respectively, three kinds of antibody materials difference
It is hypotype swine flu specific antibody H1N1、H3N2And H1N2, its material of the working electrode is that gold material or thermal decomposition are conductive
Sheet or thread is presented in macromolecule material, its pattern of the working electrode, and emphasis is that its material of the substrate is polydimethylsiloxanes
Alkane material, its surface of the substrate are the surfaces of primary form, the surface of the primary form its be intended to refer to not by any
The surface of the primary form of surface chemical modification or the material of any surface chemical modification, the structure of the device also include chain link
Formula magnetic holding device, shares six links in its structure of the chain ring type magnetic holding device, the link in strip, shaft-like or bar-shaped,
Each adjacent link is hinged to form chain link, the hexagonal cyclic structure of its appearance profile of the chain link, the hexagonal annular structure
Each drift angle its locations of structures be articulated structure position, two therein of the hexagonal annular structure is in opposite side position
Its material of link is magnetic conductivity material, and its material of the remaining four processes of the hexagonal annular structure is non-magnetic material, institute
State the material that non magnetic material refers to not responding externally-applied magnetic field strongly, one among two links of the magnetic conductivity material
Magnet exciting coil is wound with individual link, the link for being wound with magnet exciting coil respectively fills in the both sides neighbor positions of its magnet exciting coil
Pole shoe provided with a magnetic conductivity material, two pole shoes are shared, two pole shoes are directed to another magnetic conductivity material
Link, attach on its surface of the link of another magnetic conductivity material it is fixed be equiped with miniature ultrasonic transducer units, rely on electromagnetism
The chain ring type magnetic holding device socket of adhesive strength is positioned at the close position of its terminal of the micro-fluidic chip, and, it is high
Frequency vibration swings electric signal transmission cable, and one end and the miniature ultrasonic transducer units of the higher-order of oscillation electric signal transmission cable are connected to
Together；The chain ring type magnetic holding device provides a function of facilitating the device to disassemble；Its is main for the miniature ultrasonic transducer units
Function is in the test of micro-fluidic chip actual sample introduction, and the ultrasonic wave launched using it reduces sample solution and the pipeline
Inwall between interfacial tension, can be compatible, also, utilize the sample introduction end and the terminal and the miniature ultrasonic
Difference in the distance between wave transducer installation position difference and its ultrasonic intensity experienced, described in induced synthesis
Difference between its interfacial tension of sample introduction end and the terminal its interfacial tension, the interface between the micro-fluidic chip both ends
Power difference can form pressure gap between the both ends of the micro-fluidic chip, and the pressure gap can drive sample solution to described
End flow；The ultrasonic wave that its function of the miniature ultrasonic transducer units also includes being launched with it checks life contained in sample
Its absorption on the inner surface of pipeline of thing macromolecular, and then check the substrate of the dimethyl silicone polymer material its body phase pair
The effect of swallowing up of the large biological molecule；Its function of the substrate of the dimethyl silicone polymer material includes and cover plate and working electrode
And the micro-fluidic chip is together built to electrode and reference electrode, the base of the dimethyl silicone polymer material that is soft and having elasticity
Its function of piece is also included with its property to the strong absorption of ultrasonic wave, and ultrasonic wave is absorbed strongly, and thereby in the miniflow
Control chip terminal is to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end, its structure bit
During setting in the test of the micro-fluidic chip actual sample introduction in its chip the terminal of liquid flowing a remaining liquid pool
Claim terminal liquid pool, is filled with some high hydroscopic resin particles in the terminal liquid pool, its opening end of the terminal liquid pool is by breathable microporous
Film is covered.
The word of magnet exciting coil one art-recognized meanings of itself are known.
The word of magnetic conductivity material one art-recognized meanings of itself are known；The material for belonging to magnetic conductivity material is for example pure
Iron, silicon steel, soft magnetic ferrite etc..
Non magnetic material described in this case refers to the material not responded strongly to externally-applied magnetic field, belongs to the non magnetic material
The material of matter such as metallic copper, metallic zinc, ormolu, metallic aluminium, almag etc..
The be hinged word art-recognized meanings of itself field in Machine Design is known.
The gold size sensitive membrane is to be sufficiently mixed chitosan gold size solution and hypotype swine flu specific antibody solution
It is even, with point sample instrument point sample or specified structure position is coated on, and form its drying and forming-film.Hypotype in the gold size sensitive membrane
Swine flu specific antibody be horseradish peroxidase or glucose oxidase mark hypotype swine flu antibody, the gold
Glue sensitive membrane has been included as fixing above-mentioned each hypotype swine flu specific antibody and introducing complementary medium therein, the auxiliary
Property medium such as chitosan, cellulose acetate, gelatin be therein a kind of or their mixture.
The pipeline and the lateral and the manifold shape turnout in the microfluidic chip structure, in it
Footpath size may each be arbitrarily selected size, still, for as far as possible less with prepare liquid sample and reduction reagent loss etc.
Consideration, the pipeline and the lateral and the manifold shape turnout preferably select capillary level passage, institute
The passage for stating capillary level implies that its internal diameter passage suitable with the internal diameter of the capillary on ordinary meaning.The capillary is in it
The shape of cross section of portion's passage can be arbitrary shape, and the shape of cross section is for example circular, oval, square, rectangle, bar
Shape, naturally it is also possible to it is the linear of bending arbitrarily be present, also, the interior shape of the capillary is with the extension of pipeline,
The shape of cross section of different parts can also allow to be different shapes.Only for the word of capillary one, its art-recognized meanings is public
Know.
What is be related in structure is the electrode of microsize to electrode and reference electrode, and its electrode shape, which may each be, appoints
The selected shape of meaning, the arbitrarily selected shape such as square piece shape, rectangular patch, strip or round sheet etc..It is described
Art-recognized meanings to electrode and the reference electrode vocabulary of itself are known.
It is related to several liquid pools in this case microfluidic chip structure, the liquid pool is the pond shape or capsule for transitional liquid storage
Shape constructs, and its shape of the inner chamber of each liquid pool may each be arbitrarily selected shape, and the cavity shape is for example round
Cylindrical cavity shape, square column type cavity-like, oblong cavity shape or spherical hollow space shape etc..The size of the liquid pool can be any
Selected size, still, in order to as far as possible less with prepare liquid sample and reduce reagent loss, the liquid pool be preferably capable of with
The liquid pool of the microminiature of capillary matching.
Only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field,
It is known.
Various sizes, variously-shaped ultrasonic transducer are commercially available；Its size of commercially available miniature ultrasonic transducer units
It may diminish to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface its
It is known general technology for the professional in ultrasonic technology field for body.This case is not to this expansion superfluous words.
Only with regard to naked PDMS substrates itself micro-channel molding or lithographic technique for, be open-and-shut known skill
Art；Similarly, the technology of hole-opening is even more known simple technique on naked PDMS substrates.This case is not also superfluous to this expansion
Speech.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the device can also include higher-order of oscillation electric signal generator；The higher-order of oscillation electric signal transmission cable
Its other end can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, come for the professional in ultrasonic technology field
Say, be simple and known；The higher-order of oscillation electric signal generator can customize to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 5 milliwatts and 9000 milliwatts
Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with
Between 12MHz.
Only for the word of high hydroscopic resin one art-recognized meanings of itself, come for the professional of chemical field
Say, be known.
The high hydroscopic resin is commercially available.
Only for the word of breathable microporous film one art-recognized meanings of itself, for the professional of technical field of membrane,
It is known.
The breathable microporous film is commercially available.
This case device can further include some annexes certainly, and the annex is such as multiple tracks electrochemical workstation
Deng the art-recognized meanings of the multiple tracks electrochemical workstation are known.The each work being related in this case microfluidic chip structure
Electrode and to electrode and reference electrode etc., special it can get lines crossed and the multiple tracks electrochemical workstation via corresponding respectively
The corresponding interface coupled.It is described that special to get lines crossed be for by each phase of each electrode and the multiple tracks electrochemical workstation
Answer the private cable that interface is coupled to each other.The micro-fluidic chip in this case device, its structure can also include micro-valve,
The quantity of the micro-valve is unlimited, and according to being actually needed, the micro-valve can be installed in any need in the microfluidic chip structure
Position to be mounted；The word of micro-valve one is for the professional of micro fluidic chip technical field, the art-recognized meanings of itself
It is known；The micro-valve itself manufacturing technology and the use of technology is also known；The component that the micro-valve is not required.
The diameter of the working electrode can allow to be that any setting is easily installed the suitable diameter used, still,
It is recommending or say preferable its scope of the diameter between 0.1 micron to 2000 microns；The length of the working electrode can
To allow to be that any setting is easily installed the length used, it is however recommended to or to say the preferable length its scope be 1
Micron is between 15000 microns.
It is installed in by spraying or point sample instrument point sample or the coating of other appropriate process described in the working electrode surface layer
Gold size sensitive membrane, its thicknesses of layers can allow be any setting treat sample measuring liquid occur electrical signals response thickness,
It is however recommended to thickness preferable thickness is between 10 nanometers and 200 nanometers in other words.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, such as：Polypropylene,
Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, for example do
Into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and realized in the extremely short distance to ultrasonic wave
Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip
Using dimethyl silicone polymer it is used as the cover plate, and this case technical scheme is allowed.
Positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal should
Air line distance between a remaining liquid pool can be arbitrarily selected suitable distance, still, the distance as needed
Preferred value is between 3 centimetres and 7 centimetres.
Described its thickness of cover plate and substrate can allow be any setting the thickness for being easy to assembling, the thickness of recommendation or say
Preferable thickness is between 1.0 millimeters and 5.0 millimeters.Less thickness is advantageous to save material.
The application method of this case micro-fluidic chip：
Itd is proposed first based on this case and the first public new liquid stream driving principle, its application of this case micro-fluidic chip are transported
Among work, the new liquid stream driving method is determined completely without involving any additional Micropump.
The interfacial tension difference that this case is formed between micro-fluidic chip both ends caused by the ultrasonic wave,
Driving liquid stream flows in the capillary channel of the triple channel micro-fluidic chip, right respectively using multi-channel electrochemical analyzer device
Three kinds of hypotype swine flu diagnostic antigens are detected.
The specific detection of this case micro-fluidic chip is as follows using step：
1st, blood serum sample liquid is added in micro-pipe road, under ultrasonic wave driving, various hypotype swine flu antigen molecules
The hypotype swine flu of the corresponding horseradish peroxidase-labeled embedded by gold size sensitive membrane on electrode surface in each passage is special
Property antibody capture.
2nd, the hypotype swine flu specific antibody of horseradish peroxidase-labeled resists with the hypotype swine flu in blood serum sample
Original shape is into immune complex.
3rd, using multi-channel electrochemical analyzer, the electron mediators such as catechol are added, it is above-mentioned using amperometric detection
Curent change caused by reaction, it is derived from the species and content of various analytes.
4th, result is subjected to comprehensive analysis, comprehensive diagnos is carried out to hypotype swine flu antigen.
It is an advantage of the invention that its close position relies on electromagnetism adhesive strength set in the terminal of the micro-fluidic chip
The described chain ring type magnetic holding device of positioning is connect, is installed with institute's attaching in the chain ring type magnetic holding device one magnetic conductivity link micro-
Type ultrasonic transducer, the low-power launched using it, the ultrasonic wave of high-frequency band so that without surface chemical modification or
It is compatible between strong hydrophobic its tube wall of micro-fluidic chip internal pipeline and the test object aqueous solution of surface chemical modification
Property be significantly increased, this is sample liquid stream by providing a realistic possibility；Meanwhile utilize dimethyl silicone polymer substrate
Its strong absorbability to ultrasonic wave, in shorter distance, it is, from the terminal to the sample introduction end
In the very short distance of only several centimeters yardsticks, reach the rapid decrement of ultrasonic intensity, thereby described in the micro-fluidic chip
The difference of the interfacial tension is caused at both ends, and then, using the interfacial tension between the both ends difference its formed this two
Pressure gap between end, driving sample liquid stream is in the strong hydrophobic capillary channel of such a script to the terminal side
To flowing.By this case liquid stream drive scheme, entirely without must be to the substrate and its internal pipeline of the dimethyl silicone polymer material
Any surface chemical modification or chemical modification are carried out, has altogether dispensed with the trouble journey of the surface chemical modification or chemical modification
Sequence；And the equipment of traditional Micropump etc is altogether dispensed with；On the other hand, the low-power, the ultrasound of high-frequency band
Ripple, additionally it is possible to contain large biological molecule in sample in literalness naked its inner surface of pipeline of dimethyl silicone polymer substrate
On absorption, and then contain the swallow up effect of its body phase of dimethyl silicone polymer substrate to the large biological molecule；It is described anti-
Former, antibody and the Reversible binding thing of antigen and antibody are all the type for belonging to described large biological molecule certainly；Due to described
Suction-operated and the described effect of swallowing up effectively contained that therefore, dependence test result will be better able to objectively anti-
Reflect actual conditions；The effect of the low-power, high-frequency band ultrasonic wave, also include facilitating the reversible knot between antigen, antibody certainly
Close the quick of reaction to reach, this causes dependence test operation can be with than the completion of faster speed.
Due to surface chemical modification or chemistry for its relevant surfaces of the dimethyl silicone polymer substrate need not be carried out
Modified operation, therefore, this surface chemical modification layer or chemically modified layer not need to exist, then, the poly dimethyl
Its body phase interior polymer molecule of siloxanes substrate constantly diffuses to the surface automatically, migrate caused by it to the surface chemistry
The damaging influence of decorative layer or chemically modified layer is also just not present.
It is related that the technical scheme of this case has dissolved its application to dimethyl silicone polymer substrate addressed above totally
A series of technical barriers.Based on this case scheme, the very cheap polydimethyl siloxane material of this kind is just possible to micro- at this
It is prepared by fluidic chip, production, using etc. field play bigger effect.
Attached in the chain ring type magnetic holding device one magnetic conductivity link in this case structure it is fixed installed it is described miniature
Ultrasonic transducer, this architecture provides a function of facilitating the device to disassemble, in this way, the chain ring type magnetic holding device is together with it
The miniature ultrasonic transducer units attached in one magnetic conductivity link just easily can be mutually disengaged with the micro-fluidic chip, that
, the component that the part can freely depart from reuses many times in which just can circulate benignly；The architectural feature is advantageous to save
The use cost of the device.
Brief description of the drawings
Fig. 1 is the schematic diagram of this case device embodiment chip part construction, it is shown that the vertical view of this structure
The structural form of chip internal pipeline and each electrode and each related liquid pool under angle, the chain ring type is not depicted in the figure
Magnetic holding device and the miniature ultrasonic transducer units set up thereon and other annexes.
Fig. 2 be this case device chain ring type magnetic holding device its with appearance side rough under the micro-fluidic chip socket state
View, the legend and indicates the relative position relation under the socket state between each component.
Fig. 3 is the outside side view under another viewing angle of this case device；Fig. 3 viewing angle and Fig. 2's
Viewing angle is mutually perpendicular to.
In figure, 1,2,8 be the different liquid pool of three installation positions respectively, and 3 be manifold shape turnout, and 4,9,12 be installing respectively
Position is different but three laterals parallel with one another for forming UNICOM's structure in parallel, and 5 be its top layer being installed in lateral 4
Specific antibody material in gold size sensitivity membrane structure is hypotype swine flu specific antibody H1N1Working electrode, 10 be installing
The specific antibody material in its top layer gold size sensitivity membrane structure in lateral 9 is hypotype swine flu specific antibody
H3N2Working electrode, 11 be the specific antibody material in its top layer gold size sensitivity membrane structure being installed in lateral 12
For hypotype swine flu specific antibody H1N2Working electrode, 6 be to electrode, and 7 be reference electrode, and 13 be chain ring type magnetic holding device,
14 be the terminal of the micro-fluidic chip, and 15 be the sample introduction end of the micro-fluidic chip, and 16 be dimethyl silicone polymer material
The substrate of matter, 17 be cover plate, and 18 be miniature ultrasonic transducer units, and 19 be higher-order of oscillation electric signal transmission cable, and 20,27 are respectively
Positioned at the link of two magnetic conductivity materials of different structure position, the link of two magnetic conductivity materials is right each other in the structure shown here
Frontier juncture system, 21,34 be two pole shoes positioned at different structure position respectively, and 22,24,26,29,31,33 be to be in six respectively
Six pin joints of diverse location, 23,25,30,32 be the link of four non magnetic materials in four diverse locations respectively,
28 be magnet exciting coil；The chain ring type magnetic holding device structure in legend is only the legend structure of signal, actually the chain ring type electromagnetism
Clamp structure is not limited to the legend chain ring type magnetic holding device structure；Arrow in legend indicate the micro-fluidic chip its in reality
When border is run, driven by pressure at two ends difference, the flow direction of its sample liquid stream.
Embodiment
In this case that Fig. 1, Fig. 2 and Fig. 3 are shown embodiment, the structure of the device includes multichannel micro-fluidic core
Piece, the structure of the micro-fluidic chip include being bonded to each other the substrate 16 and cover plate 17 of installing together, the substrate 16 and cover plate
17 be plate object or tablet, and that face towards the cover plate 17 of the substrate 16 is contained via mould pressing process or etching technics
The channel structure of formation, the substrate 16 also containing be connected with the channel structure and pierce the substrate 16 via mould pressing process,
The window structure that etching technics or simple drilling technology are formed, is bonded to each other the substrate 16 being installed together and the cover plate 17 is common
With the micro-fluidic chip containing pipeline configuration and the liquid pool structure being attached thereto has been built into, the locations of structures of the pipeline is located at
The interface zone that the substrate 16 is bonded to each other with the cover plate 17, its side of the window is blocked by the cover plate 17 and opposite side opens,
The locations of structures of the window is exactly the locations of structures of the liquid pool, and the quantity of the liquid pool is three, and three liquid pools are respectively
Liquid pool 1, liquid pool 2, liquid pool 8, two liquid pools therein are that liquid pool 1 and liquid pool 2 its locations of structures are located at entering for the micro-fluidic chip
Sample end 15, a remaining liquid pool are when liquid pool 8 its locations of structures is located at the micro-fluidic chip actual sample introduction test in its chip
The terminal 14 of liquid flowing, the terminal 14 are located remotely from each other with the sample introduction end 15, and one end of the pipeline is mutual via this is similarly positioned in
The manifold shape turnout 3 of the interface zone of fitting is liquid pool 1 and the UNICOM of liquid pool 2 with two liquid pools positioned at sample introduction end 15 respectively,
The other end of the pipeline and a remaining liquid pool for the terminal 14 positioned at the micro-fluidic chip are the UNICOM of liquid pool 8, with
And sequentially it is respectively installed in working electrode in the pipeline on diverse location and to electrode and reference electrode, the work
Electrode is quick by conductive electrode and the gold size for having embedded hypotype swine flu specific antibody being attached on the conductive electrode
Feel film to form, the construction of the pipeline is in parallel construction, and the pipeline in parallel construction is made up of three lateral parallel connections, institute
The quantity for stating working electrode is three, and the installation position of three working electrodes is located in three laterals respectively, with
And the specific antibody in three working electrodes its top layer gold size sensitivity membrane structures be respectively can be special to hypotype swine flu antigen
Three kinds of hypotype swine flu antibody materials that the opposite sex combines, three kinds of antibody materials are hypotype swine flu specific antibody H respectively1N1、
H3N2And H1N2, for specific expansion, 5 be that specificity in its top layer gold size sensitivity membrane structure being installed in lateral 4 resists
Body material is hypotype swine flu specific antibody H1N1Working electrode, 10 be that its top layer gold size being installed in lateral 9 is quick
The specific antibody material felt in membrane structure is hypotype swine flu specific antibody H3N2Working electrode, 11 be to be installed in branch
The specific antibody material in its top layer gold size sensitivity membrane structure in pipeline 12 is hypotype swine flu specific antibody H1N2Work
Make electrode, its material of the working electrode is gold material or thermally decomposes conducting polymer material, its pattern of the working electrode
Sheet or thread is presented, emphasis is, its material of the substrate 16 is dimethyl silicone polymer material, and its surface of substrate 16 is primary
The surface of form, the surface of the primary form its be intended to refer to and do not pass through any surface chemical modification or any surface chemistry
The surface of the primary form of the modified material, the structure of the device also include chain ring type magnetic holding device 13, the chain ring type electromagnetism
Six links are shared in its structure of fixture 13, six links are link 20,23,25,27,30,32 respectively, the link 20,
23rd, 25,27,30,32 in strip, shaft-like or bar-shaped, and each adjacent link is hinged to form chain link, its appearance profile of the chain link
Hexagonal cyclic structure, its locations of structures of each drift angle 22,24,26,29,31,33 of the hexagonal annular structure are hinge
Locations of structures is connect, its material is magnetic conductivity material to two therein of the hexagonal annular structure in the link 20,27 of opposite side position
Matter, its material of the remaining four processes 23,25,30,32 of the hexagonal annular structure is non-magnetic material, the non magnetic material
Matter refers to the material not responded strongly to externally-applied magnetic field, a ring among two links 20,27 of the magnetic conductivity material
Magnet exciting coil 28 is wound with section 27, this is wound with both sides neighbor positions of the link 27 in its magnet exciting coil 28 of magnet exciting coil 28
On be respectively equiped with the pole shoe of a magnetic conductivity material, share two pole shoes, the pole shoe is pole shoe 21 and pole shoe 34 respectively,
Two pole shoes 21,34 are directed to the link 20 of another magnetic conductivity material, its table of the link 20 of another magnetic conductivity material
Attach to fix on face and be equiped with miniature ultrasonic transducer units 18, rely on the chain ring type magnetic holding device 13 of electromagnetism adhesive strength to be socketed
The close position of its terminal 14 of the micro-fluidic chip is positioned at, and, higher-order of oscillation electric signal transmission cable 19, the high frequency
One end of vibration electric signal transmission cable 19 links together with the miniature ultrasonic transducer units 18；The chain ring type magnetic holding device 13
Provide a function of facilitating the device to disassemble；Its major function of the miniature ultrasonic transducer units 18 is real in micro-fluidic chip
During the sample introduction test of border, the ultrasonic wave launched using it is opened to reduce the interface between sample solution and the inwall of the pipeline
Power, can be compatible, also, is installed using the sample introduction end 15 and the terminal 14 with the miniature ultrasonic transducer units 18
Difference in the distance between position difference and its ultrasonic intensity experienced, its boundary of sample introduction end 15 described in induced synthesis
Difference between face tension force and the terminal 14 its interfacial tension, the interfacial tension difference meeting between the micro-fluidic chip both ends
Pressure gap is formed between the both ends of the micro-fluidic chip, the pressure gap can drive sample solution to be flowed to the terminal 14
It is dynamic；Its function of miniature ultrasonic transducer units 18 also ultrasonic wave including being launched with it checks biology contained in sample greatly
Its absorption on the inner surface of pipeline of molecule, and then check the substrate 16 of the dimethyl silicone polymer material its body phase to this
The effect of swallowing up of large biological molecule；Its function of the substrate 16 of the dimethyl silicone polymer material includes and cover plate 17 and work electricity
Pole 5,10,11 and the micro-fluidic chip is together built to electrode 6 and reference electrode 7, soft poly dimethyl silicon for simultaneously having elasticity
Its function of the substrate 16 of oxygen alkane material is also included with its property to the strong absorption of ultrasonic wave, and ultrasonic wave is absorbed strongly,
And thereby realize ultrasonic intensity within micro-fluidic chip terminal 14 to the limited short distance between the sample introduction end 15
Rapid decrement, when its locations of structures is located at the test of the micro-fluidic chip actual sample introduction in its chip the terminal of liquid flowing institute
State a remaining liquid pool and be also referred to as terminal liquid pool, some high hydroscopic resin particles, the terminal liquid pool are filled with the terminal liquid pool
Its opening end is covered by breathable microporous film.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its try
The flow direction of sample liquid stream.
It is depicted without attaching installing in the chain ring type magnetic holding device 13 and one magnetic conductivity material link 20 in Fig. 1
The miniature ultrasonic transducer units 18 and higher-order of oscillation electric signal transmission cable 19, and, the grade component of magnet exciting coil 28；And
And it is attached that the higher-order of oscillation electric signal generator and multiple tracks electrochemical workstation etc. are depicted without in Fig. 1, Fig. 2 and Fig. 3
Part.
Involved miniature ultrasonic transducer units 18 are commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal transmission cable 19 is commercially available；It can also be customized to ultrasonic transducer producer.
Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also determine to relevant manufacturers
System.
Each working electrode in this example structure and can be respectively via each special electricity to electrode and reference electrode
Cable or say is being got lines crossed respectively with the corresponding cable interface of the multiple tracks electrochemical workstation as annex or saying interface connection of getting lines crossed.
The structural form of the chain ring type magnetic holding device 13 in legend is only an example, chain ring type electromagnetic clamp described in this case
The structural form of tool is not limited to the structure type in the legend.
Claims (10)
1. including the easily-disassembled hypotype swine flu multi-channel testing device of electromagnetism auxiliary part, the structure of the device includes leading to more
Road micro-fluidic chip, the structure of the micro-fluidic chip include being bonded to each other the substrate and cover plate of installing together, the substrate and
Cover plate is plate object or tablet, and that face towards the cover plate of the substrate is contained via mould pressing process or etching technics shape
Into channel structure, the substrate also containing be connected with the channel structure and pierce the substrate via mould pressing process, etch work
The window structure that skill or simple drilling technology are formed, it is bonded to each other the substrate being installed together and is built into jointly with the cover plate
Micro-fluidic chip containing pipeline configuration and the liquid pool structure being attached thereto, the locations of structures of the pipeline are located at the substrate with being somebody's turn to do
The interface zone that cover plate is bonded to each other, its side of the window is blocked by the cover plate and opposite side opens, the locations of structures of the window
It is exactly the locations of structures of the liquid pool, the quantity of the liquid pool is three, and it is micro- that its locations of structures of two liquid pools therein is located at this
The sample introduction end of fluidic chip, its locations of structures of a remaining liquid pool are located at its chip when the actual sample introduction of the micro-fluidic chip is tested
The terminal of interior liquid flowing, the terminal are located remotely from each other with the sample introduction end, and one end of the pipeline is bonded to each other via this is similarly positioned in
Interface zone manifold shape turnout respectively with the Liang Ge liquid pools UNICOM positioned at sample introduction end, the other end of the pipeline with positioned at should
A remaining liquid pool UNICOM for the terminal of micro-fluidic chip, and, sequentially it is respectively installed in different positions in the pipeline
The working electrode put and to electrode and reference electrode, the working electrode is by conductive electrode and is attached to the conduction
Property electrode on embedded hypotype swine flu specific antibody gold size sensitive membrane form, the construction of the pipeline is in parallel construction,
The pipeline in parallel construction is made up of three lateral parallel connections, and the quantity of the working electrode is three, three works
The installation position for making electrode is located in three laterals respectively, and, its top layer gold size of three working electrodes is sensitive
Specific antibody in membrane structure is the three kinds of hypotype swine flu antibody things that can be specifically bound to hypotype swine flu antigen respectively
Matter, three kinds of antibody materials are hypotype swine flu specific antibody H respectively1N1、H3N2And H1N2, its material of the working electrode is
Sheet or thread is presented in gold material or thermal decomposition conducting polymer material, its pattern of the working electrode, it is characterised in that should
Its material of substrate is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the surface of the primary form
It is intended to refer to not by any surface chemical modification or the table of the primary form of the material of any surface chemical modification
Face, the structure of the device also include chain ring type magnetic holding device, and six links are shared in its structure of the chain ring type magnetic holding device, described
Link is in strip, shaft-like or bar-shaped, and each adjacent link is hinged to form chain link, the hexagonal ring of its appearance profile of the chain link
Shape structure, its locations of structures of each drift angle of the hexagonal annular structure is articulated structure position, the hexagonal annular structure
Two therein are magnetic conductivity materials in the link of opposite side position its material, the remaining four processes of the hexagonal annular structure
Its material is non-magnetic material, and the non magnetic material refers to the material not responded strongly to externally-applied magnetic field, the magnetic conduction
Magnet exciting coil is wound with a link among two links of property material, this is wound with the link of magnet exciting coil in its excitation
The pole shoe of a magnetic conductivity material is respectively equiped with the both sides neighbor positions of coil, shares two pole shoes, two pole shoes
Be directed to the link of another magnetic conductivity material, attached on its surface of the link of another magnetic conductivity material it is fixed be equiped with it is micro-
Type ultrasonic transducer, the chain ring type magnetic holding device socket of electromagnetism adhesive strength is relied on to be positioned at the micro-fluidic chip described in it
The close position of terminal, and, higher-order of oscillation electric signal transmission cable, one end of the higher-order of oscillation electric signal transmission cable is with being somebody's turn to do
Miniature ultrasonic transducer units link together；The chain ring type magnetic holding device provides a function of facilitating the device to disassemble；Should
Its major function of miniature ultrasonic transducer units be in the test of micro-fluidic chip actual sample introduction, the ultrasonic wave launched using it come
Reduce the interfacial tension between the inwall of sample solution and the pipeline, can be compatible, also, using the sample introduction end with
And the distance between the terminal and the miniature ultrasonic transducer units installation position difference and its ultrasonic wave experienced it is strong
Difference between difference on degree, its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension, the miniflow
Interfacial tension difference between the control chip both ends can form pressure gap between the both ends of the micro-fluidic chip, the pressure
Difference can drive sample solution to the end flow；Its function of the miniature ultrasonic transducer units also includes surpassing with what it was launched
Sound wave checks in sample contained its absorption on the inner surface of pipeline of large biological molecule, and then checks the poly dimethyl
Swallow up effect of its body phase of the substrate of siloxanes material to the large biological molecule；The substrate of the dimethyl silicone polymer material its
Function includes with cover plate and working electrode and electrode and reference electrode is together built the micro-fluidic chip, and softness simultaneously has elasticity
Its function of the substrate of the dimethyl silicone polymer material is also included with its property to the strong absorption of ultrasonic wave, and ultrasonic wave is carried out
It is strong to absorb, and thereby realize ultrasonic wave within micro-fluidic chip terminal to the limited short distance between the sample introduction end
The rapid decrement of intensity, its locations of structures are located at the terminal of liquid flowing in its chip when the actual sample introduction of the micro-fluidic chip is tested
A remaining liquid pool be also referred to as terminal liquid pool, is filled with some high hydroscopic resin particles, the terminal in the terminal liquid pool
Its opening end of liquid pool is covered by breathable microporous film.
2. the easily-disassembled hypotype swine flu multi-channel testing device according to claim 1 for including electromagnetism auxiliary part,
Characterized in that, the pipeline and the lateral and the manifold shape turnout are capillary channel.
3. the easily-disassembled hypotype swine flu multi-channel testing device according to claim 1 for including electromagnetism auxiliary part,
Characterized in that, the thermal decomposition conducting polymer is the conduction formed by polyimides or polyacrylonitrile after anoxybiotic is heat-treated
Property material.
4. the easily-disassembled hypotype swine flu multi-channel testing device according to claim 1 for including electromagnetism auxiliary part,
Characterized in that, the width or diameter of the working electrode be between 0.1 micron to 2000 microns, and, the work electricity
The length of pole is between 1 micron to 15000 microns.
5. the easily-disassembled hypotype swine flu multi-channel testing device according to claim 1 for including electromagnetism auxiliary part,
Characterized in that, the thickness of the gold size sensitive membrane is between 10 nanometers and 200 nanometers.
6. the easily-disassembled hypotype swine flu multi-channel testing device according to claim 1 for including electromagnetism auxiliary part,
Characterized in that, the cover plate its material in structure is dimethyl silicone polymer material.
7. the easily-disassembled hypotype swine flu multi-channel testing device according to claim 1 for including electromagnetism auxiliary part,
Characterized in that, positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal
Air line distance between a remaining liquid pool is between 3 centimetres and 7 centimetres.
8. the easily-disassembled hypotype swine flu multi-channel testing device according to claim 1 for including electromagnetism auxiliary part,
Characterized in that, described its thickness of cover plate and substrate in structure is between 1.0 millimeters and 5.0 millimeters.
9. the easily-disassembled hypotype swine flu multi-channel testing device according to claim 1 for including electromagnetism auxiliary part,
Characterized in that, the structure of the device also includes higher-order of oscillation electric signal generator, the higher-order of oscillation electric signal transmission cable
Its other end is connected with the higher-order of oscillation electric signal generator.
10. the easily-disassembled hypotype swine flu multi-channel testing device according to claim 1 for including electromagnetism auxiliary part,
Characterized in that, its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units, between 5 milliwatts and 9000 milliwatts, this is micro-
The frequency of its ultrasonic wave operationally launched of type ultrasonic transducer is between 100KHz and 12MHz.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610628385.5A CN107649219A (en) | 2016-07-26 | 2016-07-26 | Easily-disassembled hypotype swine flu multi-channel testing device comprising electromagnetism auxiliary part |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610628385.5A CN107649219A (en) | 2016-07-26 | 2016-07-26 | Easily-disassembled hypotype swine flu multi-channel testing device comprising electromagnetism auxiliary part |
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| Publication Number | Publication Date |
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| CN107649219A true CN107649219A (en) | 2018-02-02 |
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| CN101817495A (en) * | 2010-03-25 | 2010-09-01 | 湖南大学 | Micro fluid control chip and preparation method and application thereof |
| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
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2016
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|---|---|---|---|---|
| CN101817495A (en) * | 2010-03-25 | 2010-09-01 | 湖南大学 | Micro fluid control chip and preparation method and application thereof |
| CN102478582A (en) * | 2011-10-08 | 2012-05-30 | 宁波大学 | Multi-channel microfluidic chip capable of detecting various subtype swine influenza viruses simultaneously |
| CN102645429A (en) * | 2012-04-23 | 2012-08-22 | 宁波大学 | Self-cleaning and vibration wave energy digestion link containing electrogenerated chemiluminescence analyzing and detecting device |
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Application publication date: 20180202 |
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