CN203069591U - Multi-item synchronous adjustable immunofiltration device - Google Patents
Multi-item synchronous adjustable immunofiltration device Download PDFInfo
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- CN203069591U CN203069591U CN 201320044658 CN201320044658U CN203069591U CN 203069591 U CN203069591 U CN 203069591U CN 201320044658 CN201320044658 CN 201320044658 CN 201320044658 U CN201320044658 U CN 201320044658U CN 203069591 U CN203069591 U CN 203069591U
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Abstract
The utility model discloses a multi-item synchronous adjustable immunofiltration device, and relates to the immunofiltration devices for medical examination. The multi-item synchronous adjustable immunofiltration device is provided with a filtering layer, a liquid storage tube, a bottom support, an infiltration membrane, a waste liquid absorption tube and a rubber ring, wherein the filtering layer is arranged on the liquid storage tube, the bottom of the filtering layer is filter paper, the bottom support is arranged at the lower part of the liquid storage tube, the center of the bottom support has a hole, the infiltration membrane is arranged at the lower part of the bottom support, the rubber ring is arranged under the infiltration membrane, the infiltration membrane is fixed by the bottom support and the rubber ring, the waste liquid absorption tube is filled with a water absorbent lining, the upper outside of the waste liquid absorption tube is provided with a screw thread matched with the screw thread at the lower inner side of the liquid storage tube, and a small speed regulation hole is formed above the side face of the waste liquid absorption tube. The multi-item synchronous adjustable immunofiltration device can realize high-throughput concentration of samples, can be detached and assembled voluntarily, is flexible and convenient to use, can regulate the infiltration speed as needed in the infiltration conditions, and can realize the aims of sample concentration, controllable flow rate, accurate quantification and multi-item synchronous detection. The multi-item synchronous adjustable immunofiltration device is beneficial for rapid and sensitive clinical large-scale tumor marker general investigation.
Description
Technical field
The utility model relates to the immunity percolation device for medical test, especially relates to a kind of multinomial synchronous adjustable control immunity percolation device.
Background technology
Existing immunity percolation device usually adopts antigen or the direct point sample of antibody in cellulose nitrate (NC) film, and back to be dried is standby as diafiltration membrane.Utilize the filterability of miillpore filter, make antigen-antibody reaction and washing on a special percolating device, the mode of oozing filtration membrane with liquid is finished rapidly.Usually can only detect a kind of target, be applicable to qualitative or semiquantitative detection, and accurately quantitatively limited.Because the liquid rate of percolation is very fast, and lacks the speed regulation device, therefore for antigen-antibody affinity and specific having relatively high expectations.Existing immunity percolation device mostly is the fixing cartridge design of structure, waste liquid absorption layer finite capacity, and the sensitivity that detects of the denier thing to be checked that concentrates for needs also has much room for improvement, and can not reach quick, accurate, fast, the simple requirement of immunology detection well.
Summary of the invention
The purpose of this utility model provides and can realize that the sample high flux concentrates, disassembly and assembly voluntarily, use flexibly, conveniently, percolation condition can be carried out rate of percolation as required and regulated, and realizes controlled, the accurate a kind of multinomial synchronous adjustable control immunity percolation device of quantitative and multinomial synchronous testing goal of sample concentration, flow velocity.
The utility model is provided with filtering layer, liquid storage pipe, collet, diafiltration membrane, waste liquid absorption tube and rubber band; Described filtering layer is located on the liquid storage pipe, the bottom of filtering layer is filter paper, collet is located at the liquid storage pipe bottom, the central authorities of collet are provided with the hole, and diafiltration membrane is located at the collet bottom, and rubber band is located at the diafiltration membrane below, diafiltration membrane is fixed by collet and rubber band, the inner suction wadding of filling of waste liquid absorption tube, the upper outer of waste liquid absorption tube are provided with the screw thread that matches with the inboard screw thread in liquid storage pipe below, are provided with the speed governing aperture above waste liquid absorption tube side.
Described filtering layer can adopt the bite type structure; The diameter of diafiltration membrane and the diameter of collet are preferably identical and be close to; The diameter of described rubber band is preferably identical with the diameter of diafiltration membrane.
The utlity model has following advantage:
The utility model is disassembly and assembly voluntarily, use flexible, convenient.Percolation condition can be regulated as required, realizes controlled, the accurate purpose of quantitative and multinomial synchronous detection of sample concentration, flow velocity.Be of value to quick, sensitive clinical scale tumor markers generaI investigation.
Description of drawings
Fig. 1 is that the structure of the utility model embodiment is formed synoptic diagram.
Fig. 2 is that the diafiltration membrane of the utility model embodiment is overlooked mode chart.
In Fig. 1 and 2, respectively be labeled as: 1. filtering layer, 2. liquid storage pipe, 3. collet, 4. diafiltration membrane, 5. speed governing aperture, 6. waste liquid absorption tube, 7. rubber band, 8. suction wadding, 9. the quantitative mark thing one, 10. quantitative mark thing two, 11. surveyed areas one, 12. surveyed area two, 13. surveyed area three, 14. surveyed areas four.
Embodiment
The utility model is described in further detail below in conjunction with drawings and Examples.
Referring to Fig. 1 and 2, the utility model embodiment is provided with filtering layer 1, liquid storage pipe 2, collet 3, diafiltration membrane 4, waste liquid absorption tube 6 and rubber band 7; Described filtering layer 1 is located on the liquid storage pipe 2, the bottom of filtering layer 1 is filter paper, collet 3 is located at liquid storage pipe 2 bottoms, the central authorities of collet 3 are provided with the hole, and diafiltration membrane 4 is located at collet 3 bottoms, and rubber band 7 is located at diafiltration membrane 4 belows, diafiltration membrane 4 is fixing by collet 3 and rubber band 7, the inner suction waddings 8 of filling of waste liquid absorption tube 6, the upper outer of waste liquid absorption tube 6 are provided with the screw thread that the screw thread with inboard, liquid storage pipe 2 below matches, and are provided with speed governing aperture 5 above waste liquid absorption tube 6 sides.
Described filtering layer 1 is located on the liquid storage pipe 2, and the bottom of filtering layer 1 is filter paper, and sample enters liquid storage pipe 2 after by filter paper, can remove bulky grain and collosol substance, avoids stopping up diafiltration membrane.Liquid storage pipe 2 can hold the liquid of 200 μ L, can realize the abundant mixing of sample and dilution in liquid storage pipe, is conducive to sample and fully contacts with the even of diafiltration membrane 4, reduces error.Liquid storage pipe 2 bottoms are the porose collets 3 of central authorities, and collet 3 bottoms are diafiltration membrane 4, and diafiltration membrane 4 has same diameter with collet 3 and is close to.Because collet 3 central authorities are porose, diafiltration membrane 4 can expose its antigen-antibody reaction zone.One rubber-like rubber band 7 is arranged below diafiltration membrane 4, and rubber band 7 diameters are identical with diafiltration membrane.Make rubber band 7 be close to diafiltration membrane 4, diafiltration membrane 4 is fixing by the rubber band 7 of collet 3 and its below.The waste liquid absorption tube 6 inner suction waddings 8 of filling, waste liquid absorption tube 6 upper outer are helicitic texture, can match in the helicitic texture of inboard, liquid storage pipe 2 below, and rotatable fixing.Just suction wadding 8 closely can be contacted with diafiltration membrane 4 after fixing.Because the existence of India rubber circle 7 can make liquid storage pipe 2 and waste liquid absorption tube 6 secure seal, forms a confined space.Above waste liquid absorption tube 6 sides, be provided with a speed governing aperture 5, when speed governing aperture 5 is closed, liquid can make liquid storage pipe 2 and the confined space internal gas pressure that waste liquid absorption tube 6 forms increase in infitration process, hinder under the liquid and ooze, thereby the infiltration rate degree slows down under the liquid because the existence of 8 pairs of liquid suction of suction wadding makes.When speed governing aperture 5 was opened, confined space was open, and draught head disappears, and the infiltration rate degree speeds under the liquid, therefore can regulate the flow velocity of liquid by the opening of speed governing aperture 5.Liquid storage pipe 2 can be realized both freely separation with the helicitic texture between the waste liquid absorption tube 6 and assemble.When the sample of needs detection contains denier thing to be checked, need concentrate sample, and the method that realizes sample concentration is to increase sample by the total amount of diafiltration membrane 4, the increase of total amount can produce a large amount of waste liquids, its waste liquid receptivity of existing percolating device is very limited, and the utility model is because therefore the renewable replacing of waste liquid absorption tube 6 can realize that the high flux of sample concentrates.After the immunity percolation reaction is finished, need analyze the result, existing immunity percolation device is applicable to qualitative or semi-quantitative analysis, the utility model immunity percolation device is owing to can freely dismantle, after finishing, reaction liquid storage pipe 2 and inner diafiltration membrane 4 thereof can be separated with waste liquid absorption tube 6, in liquid storage pipe 2, add enzyme reaction substrate, place Chemiluminescence Apparatus, the ratio that adds sampling point fluorescence intensity and quantitative mark thing fluorescence intensity by the observation surveyed area carries out quantitative test, the zymochemistry luminescence method has higher sensitivity and stable linear relation, therefore can carry out accurately quantitatively.Carry out qualitative or half-quantitative detection as needs, can observe by gold mark method, this method is simple to operate, does not need detecting instrument, can be applicable to the needs of community hospital's disorder in screening directly by visual inspection.
In Fig. 2, mark 9 is that quantitative mark thing one, 10 is that quantitative mark thing two, 11 is that surveyed area one, 12 is that surveyed area two, 13 is that surveyed area three, 14 is surveyed area four.
Below provide the quantitative model operation instructions:
1) reagent and material:
1. confining liquid: the cleansing solution that contains 0.2%BSA and 0.05% Tween-20.
2. cleansing solution: 50mol/L, pH7.2PBS.
3. capture antibody (capture antibody).
4. in conjunction with detection antibody (Biotin-antibody) dilution (1:5000) of biotin.
5. the horseradish peroxidase of labelled streptavidin (Streptavidin-HRP) dilution (1:5000).
6.ECL developer solution.
7.NC film.
2) method of operating:
1. carry out the point sample of capture antibody (capture antibody) and dry stand-by with diafiltration membrane (NC film) 4 activation, and in the fixed area of NC film.
2. liquid storage pipe 2 is inverted, and the NC film behind the point sample is placed on the collet 3, put into rubber band 7 fix N C films again, at last with waste liquid absorption tube 6 and liquid storage pipe 2 spiral fastenings, make the NC film be firmly secured between waste liquid absorption tube 6 and the liquid storage pipe 2 assembly working of finishing device.
3. 200 μ l confining liquids are added liquid storage by filtering layer, in 2, treat that confining liquid infiltrates waste liquid absorption tube 6.
4. sample to be measured is diluted in the 0.2%BSA solution of 200 μ l, adds liquid storage pipe 2 behind the mixing, treat that sample solution infiltrates waste liquid absorption tube 6.
5. 200 μ l Biotin-antibody dilutions are added in the liquid storage pipe 2, treat that the Biotin-antibody dilution infiltrates waste liquid absorption tube 6.
6. 200 μ l cleansing solutions are added in the liquid storage pipe 2, treat that cleansing solution infiltrates waste liquid absorption tube 6.
7. 200 μ l Streptavidin-HRP dilutions are added in the liquid storage pipe 2, treat that the Streptavidin-HRP dilution infiltrates waste liquid absorption tube 6.
8. 200 μ l cleansing solutions are added in the liquid storage pipe 2, treat that cleansing solution infiltrates waste liquid absorption tube 6.
9. liquid storage pipe 2 is separated with waste liquid absorption tube 6, and 100 μ l ECL developer solutions are added liquid storage pipe 2.
10. liquid storage pipe 2 is put into the chemiluminescence imaging system, take, the ratio that adds sampling point fluorescence intensity and quantitative mark thing fluorescence intensity by the observation surveyed area carries out quantitative test.
Below provide qualitative or sxemiquantitative pattern operation instructions:
1) reagent and material:
1. confining liquid: the cleansing solution that contains 0.2%BSA and 0.05% Tween-20.
2. cleansing solution: 50mol/L, pH7.2PBS.
3. capture antibody (capture antibody).
4. the detection antibody of nano gold mark (golden labeling antibody).
5.NC film.
2) method of operating:
1. carry out the point sample of capture antibody (capture antibody) and dry stand-by with diafiltration membrane (NC film) 4 activation, and in the fixed area of NC film.
2. liquid storage pipe 2 is inverted, and the NC film behind the point sample is placed on the collet 3, put into rubber band 7 fix N C films again, at last with waste liquid absorption tube 6 and liquid storage pipe 2 spiral fastenings, make the NC film be firmly secured between waste liquid absorption tube and the liquid storage pipe assembly working of finishing device.
3. 200 μ l confining liquids are added in the liquid storage pipe by filtering layer, treat that confining liquid infiltrates the waste liquid absorption tube.
4. sample to be measured is diluted in the 0.2%BSA solution of 200 μ l, adds liquid storage pipe behind the mixing, treat that sample solution infiltrates the waste liquid absorption tube.
5. the detection antibody (golden labeling antibody) with nano gold mark adds in the liquid storage pipe, treats that golden labeling antibody infiltrates the waste liquid absorption tube.
6. 200 μ l cleansing solutions are added in the liquid storage pipe, treat that cleansing solution infiltrates the waste liquid absorption tube.
7. observations is positive findings during the shown in red round dot of surveyed area.
Claims (3)
1. a multinomial synchronous adjustable control immunity percolation device is characterized in that being provided with filtering layer, liquid storage pipe, collet, diafiltration membrane, waste liquid absorption tube and rubber band; Described filtering layer is located on the liquid storage pipe, the bottom of filtering layer is filter paper, collet is located at the liquid storage pipe bottom, the central authorities of collet are provided with the hole, and diafiltration membrane is located at the collet bottom, and rubber band is located at the diafiltration membrane below, diafiltration membrane is fixed by collet and rubber band, the inner suction wadding of filling of waste liquid absorption tube, the upper outer of waste liquid absorption tube are provided with the screw thread that matches with the inboard screw thread in liquid storage pipe below, are provided with the speed governing aperture above waste liquid absorption tube side.
2. a kind of multinomial synchronous adjustable control immunity percolation device according to claim 1, the diameter that it is characterized in that described diafiltration membrane is identical with the diameter of collet and be close to.
3. a kind of multinomial synchronous adjustable control immunity percolation device according to claim 1 is characterized in that the diameter of described rubber band is identical with the diameter of diafiltration membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320044658 CN203069591U (en) | 2013-01-28 | 2013-01-28 | Multi-item synchronous adjustable immunofiltration device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320044658 CN203069591U (en) | 2013-01-28 | 2013-01-28 | Multi-item synchronous adjustable immunofiltration device |
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| Publication Number | Publication Date |
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| CN203069591U true CN203069591U (en) | 2013-07-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201320044658 Expired - Fee Related CN203069591U (en) | 2013-01-28 | 2013-01-28 | Multi-item synchronous adjustable immunofiltration device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105223061A (en) * | 2015-11-06 | 2016-01-06 | 长春市博坤生物科技有限公司 | A kind of biological liquid sample gathers enrichment method and device |
| CN110376373A (en) * | 2019-08-23 | 2019-10-25 | 王爱芳 | A kind of colloidal gold accelerates reaction unit and detection method |
-
2013
- 2013-01-28 CN CN 201320044658 patent/CN203069591U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105223061A (en) * | 2015-11-06 | 2016-01-06 | 长春市博坤生物科技有限公司 | A kind of biological liquid sample gathers enrichment method and device |
| CN110376373A (en) * | 2019-08-23 | 2019-10-25 | 王爱芳 | A kind of colloidal gold accelerates reaction unit and detection method |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130717 Termination date: 20220128 |
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| CF01 | Termination of patent right due to non-payment of annual fee |