CN103645314A - Test strip card capable of automatically filtering sample - Google Patents
Test strip card capable of automatically filtering sample Download PDFInfo
- Publication number
- CN103645314A CN103645314A CN201310625354.0A CN201310625354A CN103645314A CN 103645314 A CN103645314 A CN 103645314A CN 201310625354 A CN201310625354 A CN 201310625354A CN 103645314 A CN103645314 A CN 103645314A
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- China
- Prior art keywords
- examination bar
- sample
- bar
- pad
- detection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
Abstract
The invention belongs to the field of in vitro diagnosis, and particularly relates to a test strip card capable of automatically filtering a sample. The test strip card comprises a card box (3) and a test strip (2) in the card box (3). A filter pad (12) used for filtering the sample is connected between a sample pad (4) of the test strip (2) and a label pad (5). When a sample to be tested is blood, the test strip (2) in the test strip card is capable of automatically filtering red blood cells in the blood, and can be directly detected by using whole blood.
Description
Technical field
The invention belongs to in-vitro diagnosis field, the examination bar card of energy automatic fitration sample while being specifically related to a kind of sample detection.
Background technology
Blood is the conventional sample that clinical diagnosis detects.Clinically, detect blood sample and often need to use hydro-extractor, centrifuge tube etc. separated hemorrhage clear, with serum, detect.Because the red blood cell in blood has interference effect to many diagnostic assays: during determinand detects, red blood cell may suppress the combination between being paired into minute of specificity structure; Red blood cell has enzymatic activity, and this kind of enzymatic activity likely can produce by Interference Detection signal; During immunity-chromatography test strip is analyzed, red blood cell can stop the fluid sample of participating in reaction to ooze and move smoothly on examination bar.When detecting employing colloid gold test strip, red blood cell color (redness) is close or identical with collaurum Show Color (light red or aubergine), can interference detection results judgement.Scheme that utility model patent ZL03217482.9 has disclosed a kind of " the simple and direct quick disease diagnosis test strips of improvement ", introduces red blood cell filtering membrane to attempt to overcome the detection interference effect of red blood cell to colloidal gold strip between its collaurum conjugate at colloidal gold strip and nitrocellulose filter.The weak point of this patent is: these technical measures are not to start to overcome from examination bar " source " assembly (sample pad 4), it is that red blood cell filtering membrane is introduced between the label pad 5 and analyzing film 8 of colloidal gold strip (i.e. " between collaurum conjugate and the nitrocellulose filter " of test strips described in this patent), thisly from examination bar " downstream " assembly, filter erythrocytic " improvement " scheme and can not effectively overcome the interference of red blood cell to the association reaction carrying out between label pad 5 encrusting substances (golden mark-antigen/antibody) and the target checking matter from sample pad 4!
For overcoming the deficiencies in the prior art, the invention provides a kind of examination bar card that tries bar 2 in it can automatic fitration samples when sample detection.When testing sample is blood, the effective red blood cell in filtering blood of examination bar 2, just can directly detect with whole blood in described examination bar card.
Summary of the invention
Technical solution of the present invention is as follows:
Of the present invention can automatic fitration the examination bar card of sample, comprise the examination bar 2 of cartridge 3 and Qi Nei.Examination bar 2 comprises that overlap joint is fixed on sample pad 4, filter pad 12, label pad 5, analyzing film 8, the adsorptive pads 9 on end liner 1 in turn.Analyzing film 8 has to detect is with 6 and quality control band 7.The upper box face of cartridge 3 is having well 10 corresponding to examination bar sample pad 4 positions.The upper box face of cartridge 3 is having detection window 11 corresponding to examination bar analyzing film 8 positions.
The filter pad 12 of described examination bar 2 comprises hemofiltration film.
The a certain target checking matter that the label pad 5 of described examination bar 2 is coated with single labelled substance markers detects relevant single special molecular, or: each target checking matter that is coated with different label correspondence markings detects the potpourri of relevant special molecular.
The detection of described examination bar 2 is with 6 can be one, also can be many.Detection is coated with target checking matter with 6 and detects another relevant special molecular.
The quality control band 7 of described examination bar 2 is coated with Quality Control thing.Described Quality Control thing comprises that two is anti-.
The label pad 5 of examination bar 2 of the present invention is glass fibre membrane; The analyzing film 8 of described examination bar 2 is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner 1 of described examination bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
Examination bar card of the present invention according to the detection of examination bar in card with 6 and quality control band 7 colour developing situations or by instrument (as Ultraviolet Detector, fluorescence detector, magnetic signal detector etc.) the inspection test-strips with signal testing function detect with 6 and the signal of quality control band 7 realize the single or polycomponent fast detecting of sample.
During sample detection, fluid sample is applied in the well 10 of examination bar card.The checking matter fluid sample that sample pad 4 obtains by well 10 is under the pulling of adsorptive pads 9 that is positioned at examination bar 2 other ends, the capillary siphoning effect that utilizes miillpore filter slowly to examination bar 2 rear ends, ooze and move (by filter pad 12, arrive first and reach label pad 5, with after the production association reaction of label pad 5, continue to ooze move on to the detection that reaches analyzing film 8 be with 6 and quality control band 7) and complete examination bar and react.Ooze and move past in journey, the filter pad district 12 of fluid sample after being positioned at examination bar " source " assembly (sample pad 4) is filtered processing, to avoid the chaff interference in sample to cause interference to the examination bar detection reaction that is about to carry out after filter pad 12.When testing sample is whole blood sample, examination bar filter pad 12 can stop red blood cell in blood by and only allow its serum filter, filter pad 12 play separation of serum effectiveness (red blood cell not only can stop sample in examination oozing and moving smoothly on bar 2, and, when sample detection adopts colloid gold test strip, erythrocytic red color is understood redness or close observation and the judgement that can affect testing result of aubergine color showing because of the colloid gold label thing with trying on bar, thereby affects authenticity and the reliability of testing result).
The sample to be checked of examination bar card of the present invention can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., and non-clinical sample comprises the sample of food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
The present invention has following beneficial effect:
(1), when described examination bar card is for sample detection, can carry out filtration treatment to sample.When testing sample is blood sample, in examination bar card the filter pad 12 of examination bar 2 can effectively stop red blood cell by and can only allow its serum filter, it is separated hemorrhage clear that blood sample does not need with centrifugation apparatus etc., with whole blood, just can directly detect.
(2) can realize sample polycomponent fast detecting.Each target checking matter that tries the coated different label correspondence markings of label pad 5 of bar 2 in described examination bar card detects the potpourri of relevant special molecular, the detection of examination bar 2 is with 6 during for many, and examination bar card of the present invention can be realized sample polycomponent and detect simultaneously and rapidly.
(3) in described examination bar card, reagent and material do not relate to active inactivation, can preserve for a long time in room temperature.
Accompanying drawing explanation
Figure l: the plan structure figure of examination bar card of the present invention (showing that in it, one of examination strip adoption detects band)
Fig. 2: the side-looking structural drawing of examination bar in the cartridge of examination bar card of the present invention (showing that one of examination strip adoption detects band)
Fig. 3: the plan structure figure of examination bar card of the present invention (showing that in it, many of examination strip adoptions detect band)
Fig. 4: the side-looking structural drawing of examination bar in the cartridge of examination bar card of the present invention (showing that many of examination strip adoptions detect band)
Sequence number is expressed as follows:
1, end liner, 2, examination bar, 3, cartridge, 4, sample pad, 5, label pad, 6, detect band, 7, quality control band, 8, analyzing film, 9, adsorptive pads, 10, well, 11, detection window, 12, filter pad.
Embodiment
Below in conjunction with Fig. 1-4, further illustrate the present invention, those skilled in the art should be with this embodiment and accompanying drawings thereof and are limited the scope of the invention.
In Fig. 1-4, the examination bar card of described energy automatic fitration sample comprises the examination bar 2 of cartridge 3 and Qi Nei.Described examination bar 2 comprises that overlap joint is fixed on sample pad 4, filter pad 12, label pad 5, analyzing film 8, the adsorptive pads 9 on end liner 1 in turn.Analyzing film 8 has to detect is with 6 and quality control band 7.The upper box face of cartridge 3 is having well 10 corresponding to examination bar sample pad 4 positions.The upper box face of cartridge 3 is having detection window 11 corresponding to examination bar analyzing film 8 positions.
The filter pad 12 of described examination bar 2 comprises hemofiltration film.
The a certain target checking matter that the label pad 5 of described examination bar 2 is coated with single labelled substance markers detects relevant single special molecular, or: each target checking matter that is coated with different label correspondence markings detects the potpourri of relevant special molecular.
The detection of described examination bar 2 is with 6 can be one (shown in Fig. 1 and Fig. 2), can be also many (shown in Fig. 3 and Fig. 4).Detection is coated with target checking matter with 6 and detects another relevant special molecular.
The quality control band 7 of described examination bar 2 is coated with Quality Control thing.Described Quality Control thing comprises that two is anti-.
The label pad 5 of described examination bar 2 is glass fibre membrane; The analyzing film 8 of described examination bar 2 is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner 1 of described examination bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
Described examination bar 2 is any examination bar, and it includes but not limited to quanta dot mark test bar, colloid gold label examination bar, colloidal selenium marked examination bar, up-converting phosphor substance markers examination bar, nano rare earth fluorescence complex mark test bar, nano magnetic particle mark test bar, time resolution chromatography examination bar, chemiluminescence examination bar.
Examination bar card of the present invention according to the detection of examination bar in card with 6 and quality control band 7 colour developing situations or by instrument (as Ultraviolet Detector, fluorescence detector, magnetic signal detector etc.) the inspection test-strips with signal testing function detect with 6 and the signal of quality control band 7 realize the single or polycomponent fast detecting of sample.
The sample to be checked of described examination bar card can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., and non-clinical sample comprises the sample of food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
It is pointed out that examination bar card of the present invention can also have other to improve, and also can expand and replace with biochip (comprising antigen-antibody chip, protein chip, nucleic acid chip, micro-fluidic chip) etc. as the examination bar in cartridge 3.Therefore, every to any other technical scheme that is equal to replacement or equivalent transformation formation of examination bar card employing of the present invention, all drop in the protection domain of the claims in the present invention.
Claims (6)
1. the examination bar card of an energy automatic fitration sample, comprise cartridge (3) and the examination bar (2) in it, examination bar (2) comprises that overlap joint is fixed on the sample pad (4) on end liner (1) in turn, label pad (5), analyzing film (8), adsorptive pads (9), analyzing film (8) has the band of detection (6) and quality control band (7), detecting band (6) is one or more, the upper box face of cartridge (3) is having well (10) corresponding to examination bar sample pad (4) position, the upper box face of cartridge (3) is having detection window (11) corresponding to examination bar analyzing film (8) position, it is characterized in that: wherein, between the sample pad of described examination bar (4) and label pad (5), be also connected with a filter pad for filtered sample (12).
2. the examination bar card of energy automatic fitration sample according to claim 1, is characterized in that: the filter pad of described examination bar (12) comprises hemofiltration film.
According to claim 1 can automatic fitration the examination bar card of sample, it is characterized in that:
The a certain target checking matter that the label pad (5) of described examination bar (2) is coated with single labelled substance markers detects relevant single special molecular, or: each target checking matter that is coated with different label correspondence markings detects the potpourri of relevant special molecular;
The detection band (6) of described examination bar (2) is coated with target checking matter and detects another relevant special molecular;
The quality control band (7) of described examination bar (2) is coated with and comprises two anti-Quality Control things.
According to claim 1 can automatic fitration the examination bar card of sample, it is characterized in that:
The label pad (5) of described examination bar (2) is glass fibre membrane, and the analyzing film (8) of described examination bar (2) is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films;
The end liner (1) of described examination bar (2) is polyester or plastic plate;
Described cartridge (3) is that polyester, plastics or rigid paper material are made.
According to claim 1 can automatic fitration the examination bar card of sample, it is characterized in that: wherein said examination bar (2) is any examination bar, it comprises quanta dot mark test bar, colloid gold label examination bar, colloidal selenium marked examination bar, up-converting phosphor substance markers examination bar, nano rare earth fluorescence complex mark test bar, nano magnetic particle mark test bar, time resolution chromatography examination bar, chemiluminescence examination bar.
One kind as claimed in claim 1 can automatic fitration the examination bar card of sample, it is characterized in that: sample to be checked is from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease, and non-clinical sample comprises that food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detect, the sample of illicit drugs inspection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310625354.0A CN103645314A (en) | 2013-11-29 | 2013-11-29 | Test strip card capable of automatically filtering sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310625354.0A CN103645314A (en) | 2013-11-29 | 2013-11-29 | Test strip card capable of automatically filtering sample |
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| Publication Number | Publication Date |
|---|---|
| CN103645314A true CN103645314A (en) | 2014-03-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310625354.0A Pending CN103645314A (en) | 2013-11-29 | 2013-11-29 | Test strip card capable of automatically filtering sample |
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| CN (1) | CN103645314A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104142392A (en) * | 2014-08-18 | 2014-11-12 | 深圳市领治医学科技有限公司 | Trace sample test paper |
| WO2015070701A1 (en) * | 2013-11-13 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
| WO2015070699A1 (en) * | 2013-11-12 | 2015-05-21 | 成都领御生物技术有限公司 | Quantum dot-labeled test strip card |
| WO2015070702A1 (en) * | 2013-11-13 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
| CN104950089A (en) * | 2014-03-25 | 2015-09-30 | 兰州红虫生物工程有限责任公司 | Rapid melamine detection test paper strip |
| CN107179405A (en) * | 2017-07-05 | 2017-09-19 | 卢氏实验室公司 | A kind of related specific antigen detection means of the heart disease of quantitative information and its detection method |
| CN108845151A (en) * | 2018-05-29 | 2018-11-20 | 郑州左安检测科技有限公司 | A kind of test strips and its preparation method and application method detecting crystal methamphetamine |
| CN108957017A (en) * | 2018-05-29 | 2018-12-07 | 郑州左安检测科技有限公司 | A kind of test strips and its preparation method and application method detecting Benzodiazepine |
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| JPS61181963A (en) * | 1985-02-06 | 1986-08-14 | Akio Tomota | Separation of component in red blood cell |
| CN2629040Y (en) * | 2003-05-26 | 2004-07-28 | 漳州市格林沃生物技术有限公司 | Improved simple and fast epidemic diseases diagnostic test paper strap |
| CN101187665A (en) * | 2007-12-27 | 2008-05-28 | 北京博晖创新光电技术股份有限公司 | Enterovirus immunofluorescence chromatographic assay test paper and its preparation method |
| CN202583196U (en) * | 2012-05-15 | 2012-12-05 | 南京基蛋生物医药有限公司 | Novel colloidal gold whole blood detection card |
| CN202583195U (en) * | 2012-05-15 | 2012-12-05 | 南京基蛋生物医药有限公司 | Novel detecting test paper for colloidal gold |
| CN103091486A (en) * | 2011-11-01 | 2013-05-08 | 马义才 | Test strip detection system |
| CN203252974U (en) * | 2013-05-08 | 2013-10-30 | 四川南格尔生物医学股份有限公司 | Membrane-filtering type plasma separation cup |
-
2013
- 2013-11-29 CN CN201310625354.0A patent/CN103645314A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61181963A (en) * | 1985-02-06 | 1986-08-14 | Akio Tomota | Separation of component in red blood cell |
| CN2629040Y (en) * | 2003-05-26 | 2004-07-28 | 漳州市格林沃生物技术有限公司 | Improved simple and fast epidemic diseases diagnostic test paper strap |
| CN101187665A (en) * | 2007-12-27 | 2008-05-28 | 北京博晖创新光电技术股份有限公司 | Enterovirus immunofluorescence chromatographic assay test paper and its preparation method |
| CN103091486A (en) * | 2011-11-01 | 2013-05-08 | 马义才 | Test strip detection system |
| CN202583196U (en) * | 2012-05-15 | 2012-12-05 | 南京基蛋生物医药有限公司 | Novel colloidal gold whole blood detection card |
| CN202583195U (en) * | 2012-05-15 | 2012-12-05 | 南京基蛋生物医药有限公司 | Novel detecting test paper for colloidal gold |
| CN203252974U (en) * | 2013-05-08 | 2013-10-30 | 四川南格尔生物医学股份有限公司 | Membrane-filtering type plasma separation cup |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015070699A1 (en) * | 2013-11-12 | 2015-05-21 | 成都领御生物技术有限公司 | Quantum dot-labeled test strip card |
| WO2015070701A1 (en) * | 2013-11-13 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
| WO2015070702A1 (en) * | 2013-11-13 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
| CN104950089A (en) * | 2014-03-25 | 2015-09-30 | 兰州红虫生物工程有限责任公司 | Rapid melamine detection test paper strip |
| CN104142392A (en) * | 2014-08-18 | 2014-11-12 | 深圳市领治医学科技有限公司 | Trace sample test paper |
| CN104142392B (en) * | 2014-08-18 | 2015-12-09 | 深圳市领治医学科技有限公司 | A kind of trace sample Test paper |
| CN107179405A (en) * | 2017-07-05 | 2017-09-19 | 卢氏实验室公司 | A kind of related specific antigen detection means of the heart disease of quantitative information and its detection method |
| CN108845151A (en) * | 2018-05-29 | 2018-11-20 | 郑州左安检测科技有限公司 | A kind of test strips and its preparation method and application method detecting crystal methamphetamine |
| CN108957017A (en) * | 2018-05-29 | 2018-12-07 | 郑州左安检测科技有限公司 | A kind of test strips and its preparation method and application method detecting Benzodiazepine |
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Application publication date: 20140319 |