CN103675269A - Test strip card - Google Patents
Test strip card Download PDFInfo
- Publication number
- CN103675269A CN103675269A CN201310564945.1A CN201310564945A CN103675269A CN 103675269 A CN103675269 A CN 103675269A CN 201310564945 A CN201310564945 A CN 201310564945A CN 103675269 A CN103675269 A CN 103675269A
- Authority
- CN
- China
- Prior art keywords
- examination bar
- bar
- sample
- cartridge
- examination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
- G01N33/54388—Immunochromatographic test strips based on lateral flow
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Materials Engineering (AREA)
- Nanotechnology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the field of in-vitro diagnosis, and particularly relates to a test strip card which can filter samples and is provided with a storage medium (14) for storing a tested object standard curve, coefficient parameters or other detection information. The test strip card comprises a card box (3) and a test strip (2), wherein one end of the card box (3) is open to form a test strip slot (11), and the test strip (2) is in a card box (3) instant-plug structure; and a storage medium (14) is installed on the card box (3). When detecting a sample, one end of a sample suction pad (4) of the test strip (2) inserted into the card box (3) excessively protrudes out of the open end of the card box (3) so as to impregnate and absorb the sample, and an instrument with signal detection function acquires characteristic signals of a test strip (2) detection belt (7) and calculates the sample single component or multicomponent concentration in combination with tested object standard curve or coefficient parameters simultaneously read from the storage medium (14) by the instrument. The test strip card for detecting samples has is simple and quick, and has the characteristics of high sensitivity, objective result, high use flexibility and the like.
Description
Technical field
The invention belongs to in-vitro diagnosis field, be specifically related to a kind of energy self filtered sample, carry storage medium 14 standby storage checking matter typical curve or coefficient parameter etc. for detections information, combination there is instrument energy fast detecting sample one-component or the multi-component examination bar card of signal testing function.
Background technology
The immunity-chromatography test strip technology that the colloid gold test strip of take is main representative has now been widely used in the various aspects of immune detection.This technology is simple and quick, and a few minutes just can obtain result.But this technology exists following not enough:
(1) blood sample need to be isolated serum with instrument and equipment, can not detect whole blood sample.Because: 1. the red blood cell in whole blood sample can stop reactant oozing and moving smoothly on examination bar.2. red blood cell color (redness) is close or identical with collaurum Show Color (light red or aubergine), can interference detection results judgement.3. red blood cell has interference effect to many diagnostic assays, and as during: determinand detects, red blood cell may suppress the combination between being paired into minute of specificity structure; Red blood cell has enzymatic activity, and this enzymatic activity may produce by Interference Detection signal.Utility model patent ZL03217482.9 disclosed between a kind of collaurum conjugate at colloidal gold strip and nitrocellulose filter, add red blood cell filtering membrane technical scheme to attempt to overcome erythrocytic above-mentioned interference effect, not enough: these technical measures are not to start to overcome from " source " assembly of examination bar (inhaling sample pad 4), but red blood cell filtering membrane has been introduced between the label pad 6 and analyzing film 8 of colloidal gold strip (i.e. " between collaurum conjugate and the nitrocellulose filter " of test strips described in this patent), thisly from examination bar " downstream " assembly erythrocytic " improvement " scheme of filtering, can not effectively overcome red blood cell to label pad 6 encrusting substances (golden mark-antigen/antibody) and the interference of the association reaction that comes to carry out between the target checking matter of self-priming sample pad 4!
(2) can only qualitative detection sample, can not quantitatively detect sample, more can not realize sample polycomponent and quantitatively detect.The disclosed scheme of patent No. ZL03217482.9 like the prior art, realization still just sample is carried out to qualitative detection, can not carry out quantitatively detecting and the synchronous quantitatively detection of polycomponent to sample.
Adopting the quantitative checking matter concentration of checking matter standard items typical curve, is the main method of the quantitative sample concentration of current detection field.Standard curve making is technical strong, and process is numerous and diverse, be unable to do without professional and technical personnel.Message area is the storage medium of development recently, as RFID label (claiming again RFID tag), IC chip, magnetic code, bar code etc., for Information Access identification, has now been widely used in the fields such as computing machine, communication, electronics, business, communications and transportation control and management.RFID label, volume is little, and storage information capacity is large, and identification need not manual intervention, the desirable means of Information Access identification especially, but few people use it for biomedical detection field.
For overcoming the deficiencies in the prior art, the invention provides a kind of energy self filtered sample (when sample is whole blood, can filter red blood cell), adopt the examination bar card carry the information (not needing to make again checking matter typical curve when operating personnel detect sample) detections for such as the standby storage checking matter typical curve of storage medium 14 or coefficient parameter, just can realize fast the quantitative detection of sample single component or polycomponent in conjunction with the instrument with signal testing function.The present invention is for sample detection, has easy quick, highly sensitive, the feature such as result is objective.
Summary of the invention
Technical solution of the present invention is as follows:
Examination bar card of the present invention comprises cartridge 3 and examination bar 2.Examination bar 2 comprises that overlap joint is fixed on suction sample pad 4, filter pad 5, label pad 6, analyzing film 8, the adsorptive pads 9 on end liner 1 in turn.Analyzing film 8 has to detect is with 7.Detection is with 7 to be one or more.The upper box face of cartridge 3 is having detection window 12 corresponding to examination bar analyzing film 8 positions.One storage medium 14 is installed in cartridge 3.One end of cartridge 3 forms examination bar slot mouth 11 together with the upper box face opening of this cartridge end, to facilitate examination bar 2 to insert now in cartridge 3.What examination bar 2 was cartridge 3 is inserting structure from formula.During sample detection, examination bar 2 its suction sample pad 4 one end interference that are inserted in cartridge 3 are stretched out cartridge 3 openends with dipping absorption sample.
The filter pad 5 of described examination bar 2 comprises hemofiltration film.
The a certain target checking matter that the label pad 6 of described examination bar 2 is coated with single labelled substance markers detects relevant single special molecular, or: each target checking matter that is coated with different label correspondence markings detects the potpourri of relevant special molecular.
The detection of described examination bar 2 is coated with a certain target checking matter with 7 and detects another relevant special molecular, or: be coated with the potpourri that each target checking matter detects another relevant special molecular.
The checking matter typical curve that described storage medium 14 stores has various ways available, its include but not limited to checking matter standard items series concentration with
a detect bandbetween corresponding relation curve.Described
a detect bandbe defined as the detection band signal intensity that checking matter standard items series concentration records.
After the adsorptive pads 9 of examination bar 2 of the present invention, can also be overlapped with an examination bar reaction end indicating label 10.Correspondingly, these examination bar reaction end indicating label 10 corresponding positions of its cartridge 3 have view window 13.Described examination bar reaction end indicating label 10 includes but not limited to that color change interval is the pH test paper of 5-9.
The label pad 6 of examination bar 2 of the present invention is glass fibre membrane; The analyzing film 8 of described examination bar 2 is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner 1 of described examination bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
Examination bar of the present invention 2 is the disposable articles for use that make, the storage medium 14 in described cartridge 3 and cartridge 3 for batch examination bar 2 supporting make articles for use.
When examination bar of the present invention is stuck in for sample detection, examination bar 2 is current to be inserted in cartridge 3, is inserted into the interference external part dipping imbitition sample of the examination bar 2 in cartridge 3.Fluid sample is under the pulling of adsorptive pads 9 that is positioned at examination bar 2 other ends, the capillary siphoning effect that utilizes miillpore filter is slowly oozed and is moved (by filter pad 5, arrived first and reached label pad 6, with after the production association reaction of label pad 6, continue to ooze move on to the detection that reaches analyzing film 8 and be with 7) and completes examination bar and react to examination bar 2 rear ends.Ooze and move past in journey, the filter pad district 5 of fluid sample after being positioned at examination bar " source " assembly (inhaling sample pad 4) is filtered processing, to avoid the chaff interference in sample to cause interference to the examination bar detection reaction that is about to carry out after filter pad 5.A typical example is, when testing sample is whole blood sample, examination bar filter pad 5 can stop red blood cell in blood by and can only allow its serum filter, filter pad 5 play separation of serum effectiveness (red blood cell not only can stop sample in examination oozing and moving smoothly on bar 2, and, when sample detection adopts colloid gold test strip, redness or close authenticity and the reliability that affects testing result of aubergine color that erythrocytic red color can show because of the colloid gold label thing with trying on bar: while trying bar 2 for sample qualitative detection, can affect observation and the judgement of testing result, when examination bar 2 quantitatively detects for sample, can affect detecting instrument and accurately record checking matter actual signal intensity, thereby it is quantitative to affect sample concentration).
Examination bar of the present invention is stuck in examination bar checking matter and completes after the reaction of examination bar, and the detection that is gathered examination bar 2 in examination bar card by the instrument (as reading bar instrument, fluorescence detector, magnetic signal detector etc.) with signal testing function is read the checking matter typical curve that comes or coefficient parameter simultaneously and calculated and obtain sample single component or polycomponent concentration from storage medium 14 with 7 characteristic signals and in conjunction with this instrument.
The sample to be checked of examination bar card of the present invention can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., and non-clinical sample comprises the sample of food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
Compared with prior art, the present invention has following beneficial effect:
(1) described examination bar card when the sample detection, can carry out filtration treatment to sample, has reduced the interference effect that sample detection affects composition.As: when testing sample is whole blood sample, with whole blood, just can directly detect.And existing Lateral Flow Strip is while detecting blood sample, need to first isolate serum with instrument and equipments such as hydro-extractor, centrifuge tubes to detect again, can not detect whole blood sample.
(2) can Quantitative detection sample component.On examination bar card of the present invention, storage medium 14 is installed, this storage medium 14 stores the detection information such as checking matter typical curve or coefficient parameter.When testing sample completes on bar after chromatography reaction in examination, by have the detection that tries bar 2 in the instrument collection examination bar card of signal testing function with 7 characteristic signals and in conjunction with this instrument from storage medium 14 read simultaneously the checking matter typical curve that comes or coefficient parameter fast (in a few minutes) quantitatively obtain sample component concentration.And existing Lateral Flow Strip does not have this function.
(3) the examination bar 2 in examination bar card of the present invention, when employing is quantum dot-labeled, can be realized sample polycomponent simultaneously and quick and precisely quantitatively detect.Quantum dot (Quantum Dots, be called for short QDs) be the good spectral signature of a kind of tool of the development nineties in 20th century and the semiconductor nanocrystal of photochemical stability, there is fluorescence radiation efficiency high, exciting line wide ranges, spectral line of emission narrow range, fluorescence lifetime is long, and Stokes displacement is large, particle diameter and biomolecule are close, can multifunction after finishing etc. feature.The quantum dot of different-grain diameter, kind and structure can produce different characteristic wavelength and continue fluorescence peak spectrum, and the characteristic wavelength fluorescence peak spectrum that quantum dot potpourri produces is not overlapping.Adopt different quantum dots mark checking matter respective reaction molecule respectively, its potpourri is coated on the examination bar of examination bar card of the present invention and reacts with testing sample, by mensuration, try bar specific fluorescent signal, quantitative sample polycomponent concentration quick and precisely just in a few minutes.Existing Lateral Flow Strip can only qualitative detection sample, can not realize sample and quantitatively detect, and more can not realize sample polycomponent and quantitatively detect.
(4) in described examination bar card, reagent and material do not relate to active inactivation, can preserve for a long time in room temperature.
Accompanying drawing explanation
Figure l: the plan structure figure of examination bar card preferred version one of the present invention (showing detection line of examination strip adoption in it)
Fig. 2: the side-looking structural drawing of examination bar in the cartridge of examination bar card preferred version one of the present invention (showing detection line of examination strip adoption)
Fig. 3: the plan structure figure of examination bar card preferred version one of the present invention (showing many detection lines of examination strip adoption in it)
Fig. 4: the side-looking structural drawing of examination bar in the cartridge of examination bar card preferred version one of the present invention (showing many detection lines of examination strip adoption)
Fig. 5: the plan structure figure of another preferred version of examination bar card of the present invention (showing detection line of examination strip adoption in it)
Fig. 6: the side-looking structural drawing of examination bar in the cartridge of another preferred version of examination bar card of the present invention (showing detection line of examination strip adoption)
Fig. 7: the plan structure figure of another preferred version of examination bar card of the present invention (showing many detection lines of examination strip adoption in it)
Fig. 8: the side-looking structural drawing of examination bar in the cartridge of another preferred version of examination bar card of the present invention (showing many detection lines of examination strip adoption)
Sequence number is expressed as follows:
1, end liner, 2, examination bar, 3, cartridge, 4, inhale sample pad, 5, filter pad, 6, label pad, 7, detect band, 8, analyzing film, 9, adsorptive pads, 10, examination bar reaction end indicating label, 11, examination bar slot mouth, 12, detection window, 13, view window, 14, storage medium.
Embodiment
Embodiment and accompanying drawing thereof are only that those skilled in the art should not limit the scope of the invention with this in order to further illustrate the present invention below.
embodiment mono-
Fig. 1-4 explanation embodiment mono-.Embodiment mono-is one of examination bar card preferred version of the present invention.
In Fig. 1-4, the described examination bar card of described preferred version comprises cartridge 3 and examination bar 2.Described examination bar 2 comprises that overlap joint is fixed on suction sample pad 4, filter pad 5, label pad 6, analyzing film 8, the adsorptive pads 9 on end liner 1 in turn.Analyzing film 8 has to detect is with 7.The upper box face of cartridge 3 is having detection window 12 corresponding to examination bar analyzing film 8 positions.One storage medium 14 is installed in cartridge 3.One end of cartridge 3 forms examination bar slot mouth 11 together with the upper box face opening of this cartridge end, to facilitate examination bar 2 to insert now in cartridge 3.What examination bar 2 was cartridge 3 is inserting structure from formula.During sample detection, it is inhaled the openend that the 4 one end interference of sample pad stretch out cartridge 3 and draws sample with dipping to be inserted into examination bar in cartridge 32.
The filter pad 5 of described examination bar 2 comprises hemofiltration film.
The a certain target checking matter that the label pad 6 of described examination bar 2 is coated with single labelled substance markers detects relevant single special molecular, or: each target checking matter that is coated with different label correspondence markings detects the potpourri of relevant special molecular.
The detection of described examination bar 2 is with 7 can be one (shown in Fig. 1 and Fig. 2), can be also many (shown in Fig. 3 and Fig. 4).Detection is coated with a certain target checking matter with 7 and detects another relevant special molecular, or: be coated with the potpourri that each target checking matter detects another relevant special molecular.
The checking matter typical curve that described storage medium 14 stores has various ways available, its include but not limited to checking matter standard items series concentration with
a detect bandbetween corresponding relation curve.Described
a detect bandbe defined as the detection band signal intensity that checking matter standard items series concentration records.
The label pad 6 of described examination bar 2 is glass fibre membrane; The analyzing film 8 of described examination bar 2 is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner 1 of described examination bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
Described examination bar 2 is any examination bar, and it includes but not limited to quanta dot mark test bar, colloid gold label examination bar, colloidal selenium marked examination bar, up-converting phosphor substance markers examination bar, nano rare earth fluorescence complex mark test bar, nano magnetic particle mark test bar, time resolution chromatography examination bar, chemiluminescence examination bar.
Described examination bar 2 is the disposable articles for use that make.Storage medium in described cartridge 3 and cartridge 3 14 for batch examination bar 2 supporting make articles for use.
When described examination bar card is used for sample detection, examination bar 2 is current to be inserted in cartridge 3, is inserted into the interference external part dipping imbitition sample of the examination bar 2 in cartridge 3.Fluid sample completes on bar 2 after the reaction of examination bar in examination, and the detection that is gathered examination bar 2 by the instrument (as reading bar instrument, fluorescence detector, magnetic signal detector etc.) with signal testing function is read the checking matter typical curve that comes or coefficient parameter simultaneously and calculated and obtain sample single component or polycomponent concentration from examination bar card storage medium 14 with 7 characteristic signals and in conjunction with this instrument.
The sample to be checked of described examination bar card can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., and non-clinical sample comprises the sample of food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
embodiment bis-
Fig. 5-8 explanation embodiment bis-.Embodiment bis-is another preferred version of examination bar card of the present invention.
In Fig. 5-8, the described examination bar card of described preferred version comprises cartridge 3 and examination bar 2.Described examination bar 2 comprises that overlap joint is fixed on suction sample pad 4, filter pad 4, label pad 6, analyzing film 8, adsorptive pads 9, the examination bar reaction end indicating label 10 on end liner 1 in turn.Analyzing film 8 has to detect is with 7.The upper box face of cartridge 3 is having detection window 12 corresponding to examination bar analyzing film 8 positions.The upper box face of cartridge 3 is having view window 13 corresponding to examination bar reaction end indicating label 10 positions.One storage medium 14 is installed in cartridge 3.One end of cartridge 3 forms examination bar slot mouth 11 together with the upper box face opening of this cartridge end, to facilitate examination bar 2 to insert now in cartridge 3.What examination bar 2 was cartridge 3 is inserting structure from formula.During sample detection, examination bar 2 its suction sample pad 4 one end interference that are inserted in cartridge 3 are stretched out cartridge 3 openends with dipping absorption sample.
The filter pad 5 of described examination bar 2 comprises hemofiltration film.
The a certain target checking matter that the label pad 6 of described examination bar 2 is coated with single labelled substance markers detects relevant single special molecular, or: each target checking matter that is coated with different label correspondence markings detects the potpourri of relevant special molecular.
The detection of described examination bar 2 is with 7 can be one (shown in Fig. 5 and Fig. 6), can be also many (shown in Fig. 7 and Fig. 8).Detection is coated with a certain target checking matter with 7 and detects another relevant special molecular, or: be coated with the potpourri that each target checking matter detects another relevant special molecular.
Described examination bar reaction end indicating label 10 includes but not limited to that color change interval is the pH test paper of 5-9.During sample detection, the examination bar reaction end indicating label 10 of examination on bar can develop the color according to the pH condition of examination bar reaction, to point out examination bar reactant whether fully to ooze to move past to detect, is with 7, and whether the reaction of prompting examination bar is abundant, and whether its testing result is effective.
The checking matter typical curve that described storage medium 14 stores has various ways available, its include but not limited to checking matter standard items series concentration with
a detect bandbetween corresponding relation curve.Described
a detect bandbe defined as the detection band signal intensity that checking matter standard items series concentration records.
The label pad 6 of described examination bar 2 is glass fibre membrane; The analyzing film 8 of described examination bar 2 is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner 1 of described examination bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
Described examination bar 2 is any examination bar, and it includes but not limited to quanta dot mark test bar, colloid gold label examination bar, colloidal selenium marked examination bar, up-converting phosphor substance markers examination bar, nano rare earth fluorescence complex mark test bar, nano magnetic particle mark test bar, time resolution chromatography examination bar, chemiluminescence examination bar.
Described examination bar 2 is the disposable articles for use that make.Storage medium in described cartridge 3 and cartridge 3 14 for batch examination bar 2 supporting make articles for use.
When described examination bar card is used for sample detection, examination bar 2 is current to be inserted in cartridge 3, is inserted into the interference external part dipping imbitition sample of the examination bar 2 in cartridge 3.Examination bar reaction end indicating label 10 indication samples complete after the reaction of examination bar on examination bar 2, and the detection that is gathered examination bar 2 in examination bar card by the instrument (as reading bar instrument, fluorescence detector, magnetic signal detector etc.) with signal testing function is read the checking matter typical curve that comes or coefficient parameter simultaneously and calculated and obtain sample single component or polycomponent concentration from examination bar card storage medium 14 with 7 characteristic signals and in conjunction with this instrument.
The sample to be checked of described examination bar card can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., and non-clinical sample comprises the sample of food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
It is pointed out that examination bar card of the present invention can also have other to improve, as it is upper outside except being arranged on the cartridge 3 of examination bar card with the storage medium 14 of information to store detection such as checking matter typical curve, also can be directly installed on that examination bar card is interior to be tried on bar 2; Based on same principle of the present invention, the examination bar in cartridge 3 also can be expanded and replace with biochip (comprising antigen-antibody chip, protein chip, nucleic acid chip, micro-fluidic chip) etc.Therefore, every to any other technical scheme that is equal to replacement or equivalent transformation formation of examination bar card employing of the present invention, all drop in the protection domain of the claims in the present invention.
Claims (3)
1. one kind is tried bar card, comprise cartridge (3) and examination bar (2), examination bar (2) comprises that overlap joint is fixed on suction sample pad (4), label pad (6), analyzing film (8), the adsorptive pads (9) on end liner (1) in turn, analyzing film (8) has the band of detection (7), detecting band (7) is one or more, the upper box face of cartridge (3) is having detection window (12) corresponding to examination bar analyzing film (8) position, it is characterized in that:
One end of described cartridge (3) forms examination bar slot mouth (11) together with the upper box face opening of this cartridge end, examination bar (2) is inserting structure for cartridge (3) from formula, during sample detection, its suction sample pad (4) one end interference of examination bar (2) being inserted in cartridge (3) is stretched out cartridge (3) openend with dipping absorption sample;
One storage medium (14) is also installed in described cartridge (3);
Between the suction sample pad (4) of described examination bar and label pad (6), be also connected with a filter pad for filtered sample (5);
Storage medium (14) in described cartridge (3) comprises RFID label, IC chip, magnetic code or bar code;
Described storage medium (14) stores with batch examination bar (2) the checking matter typical curve that quantitatively sample concentration is used or coefficient parameter, examination bar lot number, tries bar effect phase, storage medium password, clinical indices reference value, Shi Tiao production firm information, and can read in measurand identity information, tester's information, sample title, sample number into spectrum, detection date, testing result information;
The checking matter typical curve that described storage medium (14) stores has various ways available, it comprise checking matter standard items series concentration with
a detect bandbetween corresponding relation curve; Described
a detect bandbe defined as the detection band signal intensity that checking matter standard items series concentration records;
The filter pad of described examination bar (5) comprises hemofiltration film;
The a certain target checking matter that the label pad (6) of described examination bar (2) is coated with single labelled substance markers detects relevant single special molecular, or: each target checking matter that is coated with different label correspondence markings detects the potpourri of relevant special molecular; The detection band (7) of described examination bar (2) is coated with a certain target checking matter and detects another relevant special molecular, or: be coated with the potpourri that each target checking matter detects another relevant special molecular;
The label pad (6) of described examination bar (2) is glass fibre membrane; The analyzing film (8) of described examination bar (2) is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner (1) of described examination bar (2) is polyester or plastic plate; Described cartridge (3) is that polyester, plastics or rigid paper material are made;
Described examination bar (2) is any examination bar, and it comprises quanta dot mark test bar, colloid gold label examination bar, colloidal selenium marked examination bar, up-converting phosphor substance markers examination bar, nano rare earth fluorescence complex mark test bar, nano magnetic particle mark test bar, time resolution chromatography examination bar, chemiluminescence examination bar;
Described examination bar (2) is the disposable articles for use that make, the storage medium (14) in described cartridge (3) and cartridge (3) for batch examination bar (2) supporting make articles for use;
During sample detection, examination bar (2) inserts in cartridge (3) now, the interference external part dipping that is inserted into the examination bar (2) in cartridge (3) is drawn sample, sample completes and tries after bar reaction on examination bar (2), tries to try bar (2) in bar card detect the characteristic signal of band (7) the checking matter typical curve simultaneously reading from storage medium (14) in conjunction with this instrument or coefficient parameter and calculate acquisition sample single component or polycomponent concentration by the instrument collection with signal testing function.
2. an examination bar card as claimed in claim 1, is characterized in that: the adsorptive pads (9) of wherein said examination bar (2) is also overlapped with an examination bar reaction end indicating label (10) afterwards; This examination bar reaction end indicating label (10) corresponding position of its cartridge (3) has view window (13);
Described examination bar reaction end indicating label (10) comprises that color change interval is the pH test paper of 5-9;
Storage medium (14) in described cartridge (3) comprises RFID label, IC chip, magnetic code or bar code;
Described storage medium (14) stores with batch examination bar (2) the checking matter typical curve that quantitatively sample concentration is used or coefficient parameter, examination bar lot number, tries bar effect phase, storage medium password, clinical indices reference value, Shi Tiao production firm information, and can read in measurand identity information, tester's information, sample title, sample number into spectrum, detection date, testing result information;
The checking matter typical curve that described storage medium (14) stores has various ways available, it comprise checking matter standard items series concentration with
a detect bandbetween corresponding relation curve; Described
a detect bandbe defined as the detection band signal intensity that checking matter standard items series concentration records;
The filter pad (5) of described examination bar (2) comprises hemofiltration film;
The a certain target checking matter that the label pad (6) of described examination bar (2) is coated with single labelled substance markers detects relevant single special molecular, or: each target checking matter that is coated with different label correspondence markings detects the potpourri of relevant special molecular; The detection band (7) of described examination bar (2) is coated with a certain target checking matter and detects another relevant special molecular, or: be coated with the potpourri that each target checking matter detects another relevant special molecular;
The label pad (6) of described examination bar (2) is glass fibre membrane; The analyzing film (8) of described examination bar (2) is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner (1) of described examination bar (2) is polyester or plastic plate; Described cartridge (3) is that polyester, plastics or rigid paper material are made;
Described examination bar (2) is any examination bar, and it comprises quanta dot mark test bar, colloid gold label examination bar, colloidal selenium marked examination bar, up-converting phosphor substance markers examination bar, nano rare earth fluorescence complex mark test bar, nano magnetic particle mark test bar, time resolution chromatography examination bar, chemiluminescence examination bar;
Described examination bar (2) is the disposable articles for use that make, the storage medium (14) in described cartridge (3) and cartridge (3) for batch examination bar (2) supporting make articles for use;
During sample detection, examination bar (2) inserts in cartridge (3) now, the interference external part dipping that is inserted into the examination bar (2) in cartridge (3) is drawn sample, try bar reaction end indicating label (10) and indicate sample on examination bar (2), to complete after the reaction of examination bar, by the instrument collection with signal testing function, tried to try in bar card the characteristic signal of bar (2) detection band (7) the checking matter typical curve simultaneously reading from storage medium (14) in conjunction with this instrument or coefficient parameter and calculate to obtain sample single component or polycomponent concentration.
3. an examination bar card as claimed in claim 1 or 2, it is characterized in that: sample to be checked is from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease, and non-clinical sample comprises that food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detect, the sample of illicit drugs inspection.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310564945.1A CN103675269A (en) | 2013-11-15 | 2013-11-15 | Test strip card |
| PCT/CN2014/090851 WO2015070748A1 (en) | 2013-11-15 | 2014-11-11 | Test strip card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310564945.1A CN103675269A (en) | 2013-11-15 | 2013-11-15 | Test strip card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103675269A true CN103675269A (en) | 2014-03-26 |
Family
ID=50313455
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310564945.1A Pending CN103675269A (en) | 2013-11-15 | 2013-11-15 | Test strip card |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN103675269A (en) |
| WO (1) | WO2015070748A1 (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015070704A1 (en) * | 2013-11-13 | 2015-05-21 | 成都领御生物技术有限公司 | Test bar card |
| WO2015070750A1 (en) * | 2013-11-16 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
| WO2015070748A1 (en) * | 2013-11-15 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
| WO2015070702A1 (en) * | 2013-11-13 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
| WO2015070700A1 (en) * | 2013-11-12 | 2015-05-21 | 成都领御生物技术有限公司 | Quantum dot-labeled test strip card |
| CN109459566A (en) * | 2018-12-26 | 2019-03-12 | 北京康思润业生物技术有限公司 | Immuno-chromatographic test paper strip and immuno-chromatography detection device including it |
| CN109459567A (en) * | 2018-12-26 | 2019-03-12 | 北京康思润业生物技术有限公司 | Immuno-chromatographic test paper strip and immuno-chromatography detection device including it |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110514648A (en) * | 2018-05-21 | 2019-11-29 | 博阳生物科技（上海）有限公司 | A kind of chemiluminescence analysis POCT detection device and its application |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101769925A (en) * | 2009-12-22 | 2010-07-07 | 王继华 | Method and system for intelligently identifying and reading immunity-chromatography test strip and application thereof |
| CN202583195U (en) * | 2012-05-15 | 2012-12-05 | 南京基蛋生物医药有限公司 | Novel detecting test paper for colloidal gold |
| CN202583196U (en) * | 2012-05-15 | 2012-12-05 | 南京基蛋生物医药有限公司 | Novel colloidal gold whole blood detection card |
| CN103091486A (en) * | 2011-11-01 | 2013-05-08 | 马义才 | Test strip detection system |
| CN103226145A (en) * | 2013-03-24 | 2013-07-31 | 湖南远泰生物技术有限公司 | Autonomous identification positioning and quantitative detection system of colloidal gold immunochromatography box |
| CN103645311A (en) * | 2013-11-13 | 2014-03-19 | 成都领御生物技术有限公司 | Test strip card |
| CN203789013U (en) * | 2014-05-05 | 2014-08-27 | 陈爱珍 | Device for separating legs of chickens and ducks |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102520193B (en) * | 2011-12-29 | 2014-08-13 | 深圳康美生物科技股份有限公司 | Fluorescence immunochromatographic assay and kit for quantitative detection of human cardiac troponin I (cTnI) |
| CN102565422B (en) * | 2011-12-29 | 2014-05-07 | 深圳康美生物科技股份有限公司 | Fluorescence immunochromatographic assay and kit for quantitatively detecting cardiac troponin T |
| CN203759016U (en) * | 2013-11-13 | 2014-08-06 | 成都领御生物技术有限公司 | Test strip card |
| CN203759013U (en) * | 2013-11-15 | 2014-08-06 | 成都领御生物技术有限公司 | Test strip card |
| CN103675269A (en) * | 2013-11-15 | 2014-03-26 | 成都领御生物技术有限公司 | Test strip card |
-
2013
- 2013-11-15 CN CN201310564945.1A patent/CN103675269A/en active Pending
-
2014
- 2014-11-11 WO PCT/CN2014/090851 patent/WO2015070748A1/en active Application Filing
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101769925A (en) * | 2009-12-22 | 2010-07-07 | 王继华 | Method and system for intelligently identifying and reading immunity-chromatography test strip and application thereof |
| CN103091486A (en) * | 2011-11-01 | 2013-05-08 | 马义才 | Test strip detection system |
| CN202583195U (en) * | 2012-05-15 | 2012-12-05 | 南京基蛋生物医药有限公司 | Novel detecting test paper for colloidal gold |
| CN202583196U (en) * | 2012-05-15 | 2012-12-05 | 南京基蛋生物医药有限公司 | Novel colloidal gold whole blood detection card |
| CN103226145A (en) * | 2013-03-24 | 2013-07-31 | 湖南远泰生物技术有限公司 | Autonomous identification positioning and quantitative detection system of colloidal gold immunochromatography box |
| CN103645311A (en) * | 2013-11-13 | 2014-03-19 | 成都领御生物技术有限公司 | Test strip card |
| CN203789013U (en) * | 2014-05-05 | 2014-08-27 | 陈爱珍 | Device for separating legs of chickens and ducks |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015070700A1 (en) * | 2013-11-12 | 2015-05-21 | 成都领御生物技术有限公司 | Quantum dot-labeled test strip card |
| WO2015070704A1 (en) * | 2013-11-13 | 2015-05-21 | 成都领御生物技术有限公司 | Test bar card |
| WO2015070702A1 (en) * | 2013-11-13 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
| WO2015070748A1 (en) * | 2013-11-15 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
| WO2015070750A1 (en) * | 2013-11-16 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
| CN109459566A (en) * | 2018-12-26 | 2019-03-12 | 北京康思润业生物技术有限公司 | Immuno-chromatographic test paper strip and immuno-chromatography detection device including it |
| CN109459567A (en) * | 2018-12-26 | 2019-03-12 | 北京康思润业生物技术有限公司 | Immuno-chromatographic test paper strip and immuno-chromatography detection device including it |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2015070748A1 (en) | 2015-05-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103645311A (en) | Test strip card | |
| CN203759017U (en) | Test strip card | |
| CN103630683A (en) | Test strip card | |
| CN103675269A (en) | Test strip card | |
| CN203759014U (en) | Test strip card | |
| CN103675268A (en) | Test strip card | |
| CN203759015U (en) | Test strip card | |
| CN103630685A (en) | Test strip card | |
| CN103645313A (en) | Test strip card | |
| CN203759013U (en) | Test strip card | |
| CN203759016U (en) | Test strip card | |
| JP2015522801A (en) | Clinical diagnostic system | |
| US8470608B2 (en) | Combined visual/fluorescence analyte detection test | |
| CN203759009U (en) | Quantum dot labeled test strip card | |
| CN103675266A (en) | Test strip card based on quantum dot labeling | |
| CN103645314A (en) | Test strip card capable of automatically filtering sample | |
| CN103630682A (en) | Test strip card based on quantum dot labeling | |
| CN103645312A (en) | Test strip card | |
| JP2008304477A (en) | Accessible assay system and its usage | |
| CN203606364U (en) | Test strip card | |
| CN103630681A (en) | Test strip card based on quantum dot labeling | |
| CN203606365U (en) | Test strip card | |
| US20230131712A1 (en) | Blood volume measurement with fluorescent dye | |
| CN103630684A (en) | Test strip card | |
| CN103675267A (en) | Test strip card based on quantum dot labeling |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140326 |