CN207571147U - For the test strips of fluorescence immune chromatography method detection - Google Patents
For the test strips of fluorescence immune chromatography method detection Download PDFInfo
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- CN207571147U CN207571147U CN201721302805.7U CN201721302805U CN207571147U CN 207571147 U CN207571147 U CN 207571147U CN 201721302805 U CN201721302805 U CN 201721302805U CN 207571147 U CN207571147 U CN 207571147U
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Abstract
The utility model discloses a kind of test strips for the detection of fluorescence immune chromatography method, including bottom plate and it is attached to sample pad closely coupled successively on bottom plate, bonding pad, reaction film and water absorption pad, sample pad includes the filter layer and sample absorption pad that are combined as a whole from top to bottom, and bonding pad includes the guide layer being combined as a whole from left to right and fluorescence binder course.Test strips provided by the utility model for the detection of fluorescence immune chromatography method by setting filter layer in the top of sample absorption pad, prevent some in analyte sample fluid not dissolve ingredient and enter the fenestra road in sample absorption pad blocked sample absorbed layer；And guide layer is set between fluorescence binder course and sample absorption pad so that analyte sample fluid comes into full contact with the realization of monoclonal antibody 01 of fluorescent marker, fully reacts, and then reduce error, improves the reaction sensitivity and reaction stability of whole system.
Description
Technical field
The utility model belongs to immunochemistry detection field, and in particular to a kind of examination for the detection of fluorescence immune chromatography method
Paper slip.
Background technology
The detection method of fluorescence immune chromatography method mainly has immunoturbidimetry, enzyme immunoassay (EIA), radio-immunity point at present
Analysis method (Radioimmunoassay, RIA), Electrochemiluminescince and be suitble to by the bed of the application in emergency treatment detect (such as colloid
Jin Fa, fluorescence immune chromatography method etc.).
Immunoturbidimetry：When antigen and antibody react and suitable (the general provision antibody of ratio in special dilution system
It is excessive) when, under the action of the poly- agent of rush (polyethylene glycol etc.) of the soluble immune complex of formation in dilution system, from liquid phase
It is precipitated, forms particle, reaction solution is made turbidity occur.When antibody concentration is fixed, the amount of the immune complex of formation is with sample
The increase of middle amount of antigen and increase, the turbidity of reaction solution is consequently increased.By the turbidity and series of standards that measure reaction solution
Product compare, you can calculate the content of antigen in sample.Immunoturbidimetry analysis carries out on full-automatic immunoturbidimetry analyzer,
High degree of automation suitable for the processing of clinical high-volume sample, but cannot be used for the detection of whole blood sample.
Noncompetitive enzyme immunoassay (EIA)：Microwell plate is coated with the antibody 01 of purifying, solid phase antibody is made, toward coating monoclonal antibody
Micropore in sequentially add antigen, then combined with the antibody 02 of horseradish peroxidase HRP labels, form antibody 01- antigens-enzyme
02 compound of labeling antibody, after thoroughly washing plus substrate TMB develops the color.TMB converts au bleu under the catalysis of HRP enzymes, and
Final yellow is converted under the action of acid.Antigen in the depth and sample of color is proportionate.Enzyme immunoassay (EIA) measures
45min is taken, takes longer, unsuitable clinical practice.
Radio immunoassay：Radioimmunoassay technique is the principle with competitive binding, utilizes radioactive isotope
(Ag*) and non-labeled antigen (Ag) is marked to react the competitive binding of specific antibody (Ab), is combined by measuring Ag-Ab
The radioactivity of object judges the amount of non-labeled antigen.Radiommunoassay has radiocontamination, radionuclide decay and not
The shortcomings that stablizing.
Chemiluminescence immunoassay：It will be with the immune anti-of highly sensitive chemical luminescent detecting technology and high specific
The technology that should be combined is improved on the basis of radiommunoassay and ELISA.It is pressed from both sides using one step of double antibody
Heart EIA enzyme immunoassay using chemiluminescent agent as substrate, is solid phase carrier with monoclonal antibody coating magnetic particle, increases absorption
Surface area can detach with liquid in magnetic field, simplify operating procedure.Electrochemiluminescince detection range is wide, precision is high, steady
It is qualitative good, but need expensive instrument.The other detection of bed is then easy to operate, detection time is short, result is also relatively reliable.
Fluorescent quantitation immunochromatography technique is immunofluorescence technique (Immunofluorescence technique) and is passed
System immunochromatography technique is combined a kind of quantitative novel detection technique of development innovation.The technology is retaining colloidal gold immunochromatographimethod
Outside the advantages of technical operation is easy, detection is quick, portability is strong, also enhancing technology by fluorescent tracing realizes testing result
Accurate quantification, but the detection method is big by the impurity effect of sample, the influence for the factors such as the residence time in bonding pad is short
The problem of caused detection sensitivity is not high or unstable.
Utility model content
The utility model is intended to solve at least some of the technical problems in related technologies.For this purpose, this reality
It is to provide a kind of test strips detected for fluorescence immune chromatography method with novel main purpose, it is intended to solve existing detection side
The problem of method sensitivity is unstable or not high.
The purpose of this utility model is achieved through the following technical solutions：
A kind of test strips for the detection of fluorescence immune chromatography method including bottom plate and are attached on the bottom plate close successively
Connected sample pad, bonding pad, reaction film and water absorption pad, the sample pad include the filter layer that is combined as a whole from top to bottom and
Sample absorption pad, the bonding pad include the guide layer being combined as a whole from left to right and fluorescence binder course.
Preferably, the protein solution detection line trace " T lines " of object antibody to be checked is coated with wherein having on the reaction film,
And it is coated with the nature controlling line trace " C lines " of goat anti-rabbit igg polyclonal antibody.
Preferably, it is in the ribbon perpendicular with the length of test strips wherein the detection line is parallel with nature controlling line.
Preferably, wherein the detection line is located at the 5-8mm of bonding pad one end, the nature controlling line is located at distance and detects
At line 4-7mm.
Preferably, wherein the right end of the sample pad and the left end of the guide layer in the bonding pad overlap, the fluorescence
The right end of binder course and the left end of reaction film overlap, and the left end of the water absorption pad and the right end of the reaction film overlap.
Preferably, wherein the filter layer is made of polyether sulfone materials.
Preferably, wherein being provided with multiple deflector holes on its left and right surface of perforation on the guide layer, and the guide layer
It is made of corpus fibrosum or nonwoven fabric.
Preferably, wherein the water absorption pad is blotting paper, water-absorbent besin particles are distributed on the blotting paper.
Preferably, wherein the reaction film is nitrocellulose filter or cellulose acetate film.
Preferably, wherein the bottom plate is made of PVC board, hardboard or hardboard.
Compared with prior art, the utility model has at least the following advantages：
Test strips provided by the utility model for the detection of fluorescence immune chromatography method including bottom plate and are attached to described
Sample pad closely coupled successively, bonding pad, reaction film and water absorption pad on bottom plate, the sample pad include being combined into from top to bottom
The filter layer and sample absorption pad of one, the bonding pad includes the guide layer being combined as a whole from left to right and fluorescence combines
Layer.By setting filter layer in the top of sample absorption pad, some in analyte sample fluid is prevented not dissolve ingredient and enter sample
Fenestra road in absorbed layer blocked sample absorbed layer；And guide layer is set between fluorescence binder course and sample absorption pad, make
The analyte sample fluid obtained in sample absorption pad is quickly directed in fluorescence binder course, and analyte sample fluid reaches then to be put in fluorescence binder course
Delaying infiltration rate so that analyte sample fluid comes into full contact with the realization of monoclonal antibody 01 of fluorescent marker, fully reacts, and then
Error is reduced, improves the reaction sensitivity and reaction stability of whole system.
Description of the drawings
It in order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, other attached drawings can also be obtained according to the structure shown in these attached drawings.
Fig. 1 is the main structure figure of the test strips provided by the utility model for the detection of fluorescence immune chromatography method；
Fig. 2 is the cross section overlooking structure figure of the guide layer in Fig. 1.
Wherein, 1, bottom plate；21st, filter layer；22nd, sample absorption pad；31st, guide layer；311st, deflector hole；32nd, fluorescence combines
Layer；4th, reaction film；41st, detection line；42nd, nature controlling line；5th, absorption pad.
Specific embodiment
The following is a combination of the drawings in the embodiments of the present utility model, and the technical scheme in the embodiment of the utility model is carried out
It clearly and completely describes, it is clear that described embodiment is only the part of the embodiment rather than whole of the utility model
Embodiment.
It is to be appreciated that the directional instruction (such as up, down, left, right, before and after ...) of institute in the utility model embodiment
It is only used for explaining relative position relation, motion conditions under a certain particular pose (as shown in drawings) between each component etc., such as
When the fruit particular pose changes, then directionality instruction also correspondingly changes correspondingly.
" multiple " are meant that at least two, such as two in the description of the present invention, three etc., unless otherwise
It is clearly specific to limit.
In the utility model unless specifically defined or limited otherwise, term " connection ", " fixation " etc. should do broad sense reason
Solution, for example, " fixation " can be fixedly connected or be detachably connected or integrally；It can be mechanical connection, also may be used
Be electrical connection；It can be directly connected, can also be indirectly connected by intermediary, can be the connection inside two elements
Or the interaction relationship of two elements, unless otherwise restricted clearly.It for the ordinary skill in the art, can be with
Concrete meaning of the above-mentioned term in the utility model is understood as the case may be.
As shown in Figure 1, a kind of test strips for the detection of fluorescence immune chromatography method, including bottom plate 1 and are attached on bottom plate 1
Closely coupled sample pad, bonding pad, reaction film 4 and water absorption pad 5 successively, the sample pad include being combined as a whole from top to bottom
Filter layer 21 and sample absorption pad 22, the bonding pad includes the guide layer 31 that is combined as a whole from left to right and fluorescence combines
Layer 32.
There is 41 trace of protein solution detection line " T lines " for being coated with object antibody to be checked and coating wherein on reaction film 4
There is 42 trace of nature controlling line " C lines " of goat anti-rabbit igg polyclonal antibody antibody.
Wherein detection line 41 is parallel with nature controlling line 42, is in the ribbon perpendicular with the length of test strips.
Wherein detection line 41 is located at away from bonding pad one end 5-8mm, and the nature controlling line 42 is located at the 4- apart from detection line 41
At 7mm, by controlling the distance of detection line 41 and bonding pad and the distance between with nature controlling line 42, being conducive to further carry
The sensitivity of the high ELISA test strip and precision.
The left end overlap joint of guide layer 31 wherein in the right end and bonding pad of sample pad, the right end of fluorescence binder course 32 with it is anti-
The left end of film 4 is answered to overlap, the left end of water absorption pad 5 is overlapped with the right end of reaction film 4, this is provided with conducive to analyte sample fluid in test paper
Seamless diffusion in item ensures the accuracy of detection.
Since the test strips are analyzed for precision instrument, to the more demanding of analyte sample fluid, the utility model institute thus
Filter layer 21 in the test strips for the detection of fluorescence immune chromatography method provided is made of polyether sulfone materials, i.e., using polyether sulfone
Filter membrane, polyethersulfone membranes (PES films) are made of polyether sulfone superfine fibre hot melt is sticked together, and belong to in-depth filtration
A kind of coating materials, with following features:Using food-grade isotactic polypropylene as raw material, production overall process is without any additive；Physics,
Stable chemical performance has good compatibility；Aperture with series, porosity is high, pollutant holding capability is big, can recoil and high temperature disappears
Poison；Resistance to pressure is good, and the filter layer 21 made using polyether sulfone materials is used in the test strips so that miscellaneous in analyte sample fluid
Matter can obtain more preferable finer processing, ensure that the stability and accuracy of detection.
As shown in Fig. 2, being provided with the multiple deflector holes 311 for penetrating through its left and right surface on wherein described guide layer 31, this is led
Discharge orifice 311 is bellmouth left large and right small, and guide layer 31 has the directive corpus fibrosum of tool or nonwoven fabric to form, by multiple
Deflector hole 311 can make guide layer have good permeability and flow conductivity, so that the analyte sample fluid in sample pad is fast
It is transferred to fluorescence binder course 32 fastly so that sample prepare liquid fully connects in fluorescence binder course 32 with fluorescent labeled antibody realization
It touches, fully reaction, further and then reduces error, improve the reaction sensitivity and reaction stability of whole system
The water absorption pad 5 is blotting paper, water-absorbent besin particles is distributed on blotting paper, which replaced with water
New type resin system of the organic solvent as decentralized medium.It is integrated with water, forms solution, after water volatilization, form resin mold material
Material, with excellent water imbibition, this is provided with conducive to the water absorption of increase water absorption pad so that the test strips have more fully
Reaction process further improves sensitivity and the accuracy of the ELISA test strip.
Wherein described reaction film 4 is nitrocellulose filter or cellulose acetate film.
Wherein described bottom plate 1 is made of PVC board, hardboard or hardboard.
Test strips provided by the utility model for the detection of fluorescence immune chromatography method, testing principle are：
The kit (fluorescence immune chromatography method) of this fluorescence immune chromatography method detection is using fluorescence double antibody sandwich method：It will be single
02 direct coated of clonal antibody is in being used as detection line 41 on reaction film 4, goat anti-rabbit igg polyclonal antibody coating is as nature controlling line
42, using fluorescein-labeled monoclonal antibody 01 and rabbit igg antibody, detected using immunochromatography double antibody sandwich method to be measured
Antigen in sample.
During test, sample is added drop-wise in the sample pad of test strips, is chromatographed under capillary effect, to be measured anti-in sample
Original is combined with fluorescent labeled antibody, is resisted together with the rabbit igg of fluorescent marker more, is diffused to test section and quality control region, coated
Monoclonal antibody 02 (when containing antigen in sample) and the capture of goat anti-rabbit igg polyclonal antibody, and compound is formed, form detection line
41 and nature controlling line 42.Under the action of exciting light, fluorescent material emits the optical signal of certain wavelength, is known by Immunofluorescence test instrument
Not.Due to the difference of determined antigen concentration, make the amount of double antibody " sandwich " compound that test section is formed different, lead to fluorescein
Penetrate the difference of optical signal value.By determined antigen concentration and fluorescence signal intensity fitted dose-response curve, you can measured not by this
Know the concentration of determined antigen in sample.
More than, the only preferable specific embodiment of the utility model, but the protection domain of utility model is not limited to
This, in the technical scope that any one skilled in the art discloses in the utility model, the variation that can readily occur in
Or replace, it should be covered within the scope of the utility model.Therefore, the scope of protection of the utility model should be with right
Subject to the protection domain of claim.
Claims (4)
1. a kind of test strips for the detection of fluorescence immune chromatography method including bottom plate and are attached on the bottom plate close phase successively
Sample pad, bonding pad, reaction film and water absorption pad even, which is characterized in that the sample pad includes being combined as a whole from top to bottom
Filter layer and sample absorption pad, the bonding pad includes the guide layer that is combined as a whole from left to right and fluorescence binder course；
On the reaction film there is the protein solution detection line trace " T lines " for being coated with object antibody to be checked and be coated with goat-anti
The nature controlling line trace " C lines " of rabbit igg polyclonal antibody；
The detection line is parallel with nature controlling line, is in the ribbon perpendicular with the length of test strips；
The detection line is located at the 5-8mm of bonding pad one end, and the nature controlling line is located at detection line 4-7mm；
The left end of guide layer in the right end of the sample pad and the bonding pad overlaps, the right end of the fluorescence binder course with it is anti-
The left end of film is answered to overlap, the left end of the water absorption pad and the right end of the reaction film overlap；
The filter layer is made of polyether sulfone materials；
The multiple deflector holes for penetrating through its left and right surface are provided on the guide layer, which is bellmouth left large and right small,
And the guide layer is made of corpus fibrosum or nonwoven fabric.
2. the test strips according to claim 1 for the detection of fluorescence immune chromatography method, which is characterized in that the water absorption pad
For blotting paper, water-absorbent besin particles are distributed on the blotting paper.
3. the test strips according to claim 2 for the detection of fluorescence immune chromatography method, which is characterized in that the reaction film
For nitrocellulose filter or cellulose acetate film.
4. it is according to claim 3 for fluorescence immune chromatography method detection test strips, which is characterized in that the bottom plate by
PVC board, hardboard or hardboard are formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721302805.7U CN207571147U (en) | 2017-09-30 | 2017-09-30 | For the test strips of fluorescence immune chromatography method detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721302805.7U CN207571147U (en) | 2017-09-30 | 2017-09-30 | For the test strips of fluorescence immune chromatography method detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207571147U true CN207571147U (en) | 2018-07-03 |
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ID=62694833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721302805.7U Active CN207571147U (en) | 2017-09-30 | 2017-09-30 | For the test strips of fluorescence immune chromatography method detection |
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| Country | Link |
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| CN (1) | CN207571147U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111579790A (en) * | 2020-05-27 | 2020-08-25 | 中国农业科学院烟草研究所 | Test paper strip for detecting pendimethalin |
| CN112881696A (en) * | 2021-01-13 | 2021-06-01 | 北京中检葆泰生物技术有限公司 | Detection method and system for stably detecting aflatoxin content |
-
2017
- 2017-09-30 CN CN201721302805.7U patent/CN207571147U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111579790A (en) * | 2020-05-27 | 2020-08-25 | 中国农业科学院烟草研究所 | Test paper strip for detecting pendimethalin |
| CN111579790B (en) * | 2020-05-27 | 2023-08-04 | 中国农业科学院烟草研究所 | Test strip for detecting pendimethalin |
| CN112881696A (en) * | 2021-01-13 | 2021-06-01 | 北京中检葆泰生物技术有限公司 | Detection method and system for stably detecting aflatoxin content |
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