CN111579790A - Test paper strip for detecting pendimethalin - Google Patents
Test paper strip for detecting pendimethalin Download PDFInfo
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- CN111579790A CN111579790A CN202010462269.7A CN202010462269A CN111579790A CN 111579790 A CN111579790 A CN 111579790A CN 202010462269 A CN202010462269 A CN 202010462269A CN 111579790 A CN111579790 A CN 111579790A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
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- G01N2430/00—Assays, e.g. immunoassays or enzyme assays, involving synthetic organic compounds as analytes
- G01N2430/20—Herbicides, e.g. DDT
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Abstract
The invention provides a test strip for detecting pendimethalin, and particularly relates to the field of pendimethalin detection; the sample pad is sequentially provided with a first absorption layer, an antibacterial layer and a second absorption layer from top to bottom, and the edges of the first absorption layer and the second absorption layer are subjected to hot melting edge sealing treatment; the combination pad is sequentially provided with a first dust-free paper layer, a water absorption layer and a second dust-free paper layer from top to bottom, and both sides of the combination pad are subjected to edge covering treatment; the absorption pad consists of upper dust-free paper, lower dust-free paper and a Super Absorbent Polymer (SAP) absorption unit, the SAP absorption unit is arranged between the upper dust-free paper and the lower dust-free paper, and the edges of the upper dust-free paper and the lower dust-free paper are subjected to hot melting edge sealing treatment; the invention has sensitive detection and can improve the detection efficiency.
Description
Technical Field
The invention belongs to the field of pendimethalin detection, and particularly relates to a test strip for detecting pendimethalin.
Background
Pendimethalin belongs to dinitroaniline herbicides, is an orange crystalline solid in pure form, has a relative density of 1.19(25 ℃), has a solubility of 0.33mg/L in water (pH 7, 20 ℃), is easily soluble in benzene, toluene and chloroform, and is slightly soluble in petroleum ether and the like. The volatility is small, the photolysis is not easy, and the stability to acid and alkali is realized. As a systemic conduction type selective preemergence herbicide, the herbicide is widely applied to control of annual weeds in cotton, corn, rice, potato, soybean, peanut, tobacco and vegetable fields. Pendimethalin, which causes the development of thyroid follicular adenomas in mice, is highly toxic to fish and invertebrates in water and is also a potential carcinogen in humans, has been listed by the U.S. Environmental Protection Agency (EPA) as a persistent bioaccumulative toxic substance (PBT).
The non-standard use of pendimethalin can cause the pesticide residue in agricultural products to exceed the standard, and the personal safety of consumers is influenced. In addition, with the increasing importance of pesticide residue in various countries in the world, strict limit standards are established for pendimethalin residue in various agricultural and sideline products. The pendimethalin residue exceeds the standard, and the foreign trade of agricultural products and food in China is certainly influenced. Conventional pendimethalin is typically measured by chromatography, such as gas chromatography. Although the chromatography is accurate in detection, the pretreatment is complex, the time is long, the detection cost is high, and the method cannot meet the requirements of rapid field detection and emergency disposal of agricultural and sideline products.
In view of the defects of the prior art, a test strip for detecting pendimethalin is needed, the detection is sensitive, and the detection efficiency can be improved.
Disclosure of Invention
The invention aims to provide a test strip for detecting pendimethalin, which is sensitive in detection and can improve the detection efficiency.
The invention provides the following technical scheme:
a test strip for detecting pendimethalin comprises a substrate, wherein a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad are sequentially arranged on the substrate; the combination pad comprises detection microspheres and quality control microspheres, wherein the detection microspheres are fluorescent microspheres coated with pendimethalin monoclonal antibody on the surface, and the quality control microspheres are fluorescent microspheres coated with rabbit anti-marker protein on the surface; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is coated with pendimethalin antigen, and the quality control line is coated with an anti-rabbit antibody;
the sample pad is sequentially provided with a first absorption layer, an antibacterial layer and a second absorption layer from top to bottom, and the edges of the first absorption layer and the second absorption layer are subjected to hot melting edge sealing treatment;
the combination pad is sequentially provided with a first dust-free paper layer, a water absorption layer and a second dust-free paper layer from top to bottom, and both sides of the combination pad are subjected to edge covering treatment;
the absorption pad consists of upper dust-free paper, lower dust-free paper and a Super Absorbent Polymer (SAP) absorption unit, the SAP absorption unit is arranged between the upper dust-free paper and the lower dust-free paper, and the edges of the upper dust-free paper and the lower dust-free paper are subjected to hot melting edge sealing treatment;
preferably, the first absorption layer and the second absorption layer are made of high-loft non-woven fabrics; the antibacterial layer is formed by interweaving warp yarns and weft yarns, and the warp yarns and the weft yarns are made of nano silver fibers;
preferably, the nano silver fiber consists of silver particles and synthetic fibers, and the particle size of the nano silver particles is 1 to 15 nanometers; and the content of the nano silver in the nano silver fiber is 90-280 mg/kg.
Preferably, the SAP absorption units are in a dot-shaped array, that is, the SAP absorption units are arranged at intervals;
preferably, the water absorbing layer is a layer with the thickness of 0.2 mm formed by interlacing N-propyl ethylenediamine (PSA) fibers into a net shape;
preferably, the upper dust-free paper and the lower dust-free paper are uniformly mixed by wood pulp fibers and polyolefin fibers, and then the dust-free paper with the thickness of 0.2 mm is obtained through a dry paper making process.
The invention has the beneficial effects that:
according to the invention, two sides of the test strip are sealed, so that the test solution is prevented from leaking, and the influence on the detection efficiency due to leakage is reduced. The SAP absorption units are beneficial to the rapid absorption of the test solution, and the SAP absorption units are arranged at intervals to improve the permeability and the flow conductivity of the test solution and improve the detection efficiency; the water absorption layer is a 0.2 mm thick layered object formed by staggering PSA fibers in a net shape, so that the sample solution combining area and the permeation efficiency can be improved, and the detection efficiency and the sensitivity can be improved.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a block diagram of the present invention;
labeled as: 1. a substrate; 2. a sample pad; 3. a bonding pad; 4. a nitrocellulose membrane; 5. an absorbent pad; 6. a first absorbent layer; 7. a second absorbent layer; 8. an antimicrobial layer; 9. a first dust-free paper layer; 10. a second dust-free paper layer; 11. a water-absorbing layer; 12. detecting lines; 13. a quality control line; 14. a dust-free paper layer is arranged; 15. a lower dust-free paper layer; 16. SAP super absorbent resin absorbent unit.
Detailed Description
Referring to fig. 1, the test strip for detecting pendimethalin comprises a substrate 1, wherein a sample pad 2, a binding pad 3, a nitrocellulose membrane 4 and an absorption pad 5 are sequentially arranged on the substrate 1; the combination pad 2 comprises detection microspheres and quality control microspheres, wherein the detection microspheres are fluorescent microspheres coated with pendimethalin monoclonal antibody on the surface, and the quality control microspheres are fluorescent microspheres coated with rabbit anti-marker protein on the surface; the nitrocellulose membrane 4 is provided with a detection line 12 and a quality control line 13, the length of the detection line 12 and the length of the quality control line 13 are both the same as the width of the nitrocellulose membrane, the detection line 12 is coated with pendimethalin antigen, and the quality control line 13 is coated with anti-rabbit antibody; the sample pad 2 is sequentially provided with a first absorption layer 6, an antibacterial layer 8 and a second absorption layer 7 from top to bottom, the edges of the first absorption layer 6 and the second absorption layer 7 are subjected to hot melting edge sealing treatment, two sides of a paper strip are sealed, test solution cannot leak, the using effect of detection is ensured, and the influence on the detection efficiency due to leakage is reduced; the first absorption layer 6 and the second absorption layer 7 are made of high-capacity non-woven fabrics, so that the permeation efficiency can be improved, and the detection efficiency is favorably improved; the antibacterial layer 8 is formed by interweaving warp yarns and weft yarns, the warp yarns and the weft yarns are made of nano silver fibers, and harmful substances such as bacteria and the like in the test solution can be effectively removed through the antibacterial layer;
example 2:
a test strip for detecting pendimethalin comprises a substrate 1, wherein a sample pad 2, a combination pad 3, a nitrocellulose membrane 4 and an absorption pad 5 are sequentially arranged on the substrate 1; the combination pad 2 comprises detection microspheres and quality control microspheres, wherein the detection microspheres are fluorescent microspheres coated with pendimethalin monoclonal antibody on the surface, and the quality control microspheres are fluorescent microspheres coated with rabbit anti-marker protein on the surface; the nitrocellulose membrane 4 is provided with a detection line 12 and a quality control line 13, the length of the detection line 12 and the length of the quality control line 13 are both the same as the width of the nitrocellulose membrane, the detection line 12 is coated with pendimethalin antigen, and the quality control line 13 is coated with anti-rabbit antibody; the sample pad 2 is sequentially provided with a first absorption layer 6, an antibacterial layer 8 and a second absorption layer 7 from top to bottom, the edges of the first absorption layer 6 and the second absorption layer 7 are subjected to hot melting edge sealing treatment, two sides of a paper strip are sealed, test solution cannot leak, the using effect of detection is ensured, and the influence on the detection efficiency due to leakage is reduced; the first absorption layer 6 and the second absorption layer 7 are made of high-capacity non-woven fabrics, so that the permeation efficiency can be improved, and the detection efficiency is favorably improved; the antibacterial layer 8 is formed by interweaving warp yarns and weft yarns, the warp yarns and the weft yarns are made of nano silver fibers, and harmful substances such as bacteria and the like in the test solution can be effectively removed through the antibacterial layer; the combination pad 9 is sequentially provided with a first dust-free paper layer 9, a water absorption layer 11 and a second dust-free paper layer 10 from top to bottom, edge covering treatment is carried out on two sides of the combination pad 9, two sides of the combination pad 9 are sealed, test solution cannot leak, the use effect of detection is ensured, and the influence on detection efficiency due to leakage is reduced; the water absorption layer 11 is a layer with the thickness of 0.2 mm formed by staggering PSA fibers in a net shape, and the net structure can improve the sample solution combining area and the permeation efficiency and is beneficial to improving the detection efficiency and the sensitivity; the dust-free paper is prepared by uniformly mixing wood pulp fibers and polyolefin fibers and then performing a dry paper making process to obtain the dust-free paper with the thickness of 0.2 mm;
example 3:
a test strip for detecting pendimethalin comprises a substrate 1, wherein a sample pad 2, a combination pad 3, a nitrocellulose membrane 4 and an absorption pad 5 are sequentially arranged on the substrate 1; the combination pad 2 comprises detection microspheres and quality control microspheres, wherein the detection microspheres are fluorescent microspheres coated with pendimethalin monoclonal antibody on the surface, and the quality control microspheres are fluorescent microspheres coated with rabbit anti-marker protein on the surface; the nitrocellulose membrane 4 is provided with a detection line 12 and a quality control line 13, the length of the detection line 12 and the length of the quality control line 13 are both the same as the width of the nitrocellulose membrane, the detection line 12 is coated with pendimethalin antigen, and the quality control line 13 is coated with anti-rabbit antibody; the sample pad 2 is sequentially provided with a first absorption layer 6, an antibacterial layer 8 and a second absorption layer 7 from top to bottom, the edges of the first absorption layer 6 and the second absorption layer 7 are subjected to hot melting edge sealing treatment, two sides of a paper strip are sealed, test solution cannot leak, the use effect of detection is ensured, and the influence on the detection efficiency due to leakage is reduced; the first absorption layer 6 and the second absorption layer 7 are made of high-capacity non-woven fabrics, so that the permeation efficiency can be improved, and the detection efficiency is favorably improved; the antibacterial layer 8 is formed by interweaving warp yarns and weft yarns, the warp yarns and the weft yarns are made of nano silver fibers, and harmful substances such as bacteria and the like in the test solution can be effectively removed through the antibacterial layer; the combination pad 9 is sequentially provided with a first dust-free paper layer 9, a water absorption layer 11 and a second dust-free paper layer 10 from top to bottom, edge covering treatment is carried out on two sides of the combination pad 9, two sides of the combination pad 9 are sealed, liquid leakage can be avoided, reliability of detection is ensured, and influence on detection caused by leakage problems can be avoided; the water absorption layer 11 is formed by PSA fiber in a staggered and net shape to form a layer object with the thickness of 0.2 mm, and the net structure can improve the sample solution combining area and the permeation efficiency, thereby being beneficial to improving the detection efficiency and the sensitivity; the dust-free paper is prepared by uniformly mixing wood pulp fibers and polyolefin fibers and then performing a dry paper making process to obtain the dust-free paper with the thickness of 0.2 mm. The absorption pad 14 consists of upper dust-free paper 14, lower dust-free paper 15 and an SAP absorption unit 16, the SAP absorption unit 16 is arranged between the upper dust-free paper 14 and the lower dust-free paper 15, and the edges of the upper dust-free paper 14 and the lower dust-free paper 15 are subjected to hot melting edge sealing treatment; the dust-free paper is prepared by uniformly mixing wood pulp fibers and polyolefin fibers and then performing a dry paper making process to obtain the dust-free paper with the thickness of 0.2 mm; the SAP absorption units 16 are in a dot-like array, i.e., the SAP absorption units 16 are spaced from each other; the permeability and the conductivity of the test solution can be improved, and the complete absorption of the test solution is facilitated;
although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art will understand that various changes, modifications and substitutions can be made without departing from the spirit and scope of the invention as defined by the appended claims. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. The test strip for detecting pendimethalin is characterized in that: the kit comprises a substrate, wherein a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad are sequentially arranged on the substrate; the combination pad comprises detection microspheres and quality control microspheres, wherein the detection microspheres are fluorescent microspheres coated with pendimethalin monoclonal antibody on the surface, and the quality control microspheres are fluorescent microspheres coated with rabbit anti-marker protein on the surface; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is coated with pendimethalin antigen, and the quality control line is coated with an anti-rabbit antibody;
the sample pad is sequentially provided with a first absorption layer, an antibacterial layer and a second absorption layer from top to bottom, and the edges of the first absorption layer and the second absorption layer are subjected to hot melting edge sealing treatment;
the combination pad is sequentially provided with a first dust-free paper layer, a water absorption layer and a second dust-free paper layer from top to bottom, and both sides of the combination pad are subjected to edge covering treatment;
the absorption pad is composed of upper dust-free paper, lower dust-free paper and a Super Absorbent Polymer (SAP) absorption unit, the SAP absorption unit is arranged between the upper dust-free paper and the lower dust-free paper, and the edges of the upper dust-free paper and the lower dust-free paper are subjected to hot melting edge sealing treatment.
2. The test strip for detecting pendimethalin according to claim 1, wherein: the first absorption layer and the second absorption layer adopt high-rise non-woven fabrics; the antibacterial layer is formed by interweaving warp yarns and weft yarns, and the warp yarns and the weft yarns are made of nano silver fibers.
3. The test strip for detecting pendimethalin according to claim 2, wherein: the nano silver fiber consists of silver particles and synthetic fiber, and the particle size of the nano silver particles is 1-15 nanometers; and the content of the nano silver in the nano silver fiber is 90-280 mg/kg.
4. The test strip for detecting pendimethalin according to claim 1, wherein: the SAP absorption units are in a dot-shaped array, namely the SAP absorption units are arranged at intervals.
5. The test strip for detecting pendimethalin according to claim 1, wherein: the water absorbing layer is a layer with the thickness of 0.2 mm formed by interlacing PSA (polystyrene sulfone) terephthalamide fibers into a net shape.
6. The test strip for detecting pendimethalin according to claim 1, wherein: the upper dust-free paper and the lower dust-free paper are uniformly mixed by wood pulp fiber and polyolefin fiber, and then the dust-free paper with the thickness of 0.2 mm is obtained through a dry paper making process.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010462269.7A CN111579790B (en) | 2020-05-27 | 2020-05-27 | Test strip for detecting pendimethalin |
| NZ771345A NZ771345A (en) | 2020-05-27 | 2020-12-18 | Test strip for detecting pendimethalin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010462269.7A CN111579790B (en) | 2020-05-27 | 2020-05-27 | Test strip for detecting pendimethalin |
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| Publication Number | Publication Date |
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| CN111579790A true CN111579790A (en) | 2020-08-25 |
| CN111579790B CN111579790B (en) | 2023-08-04 |
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| CN202010462269.7A Active CN111579790B (en) | 2020-05-27 | 2020-05-27 | Test strip for detecting pendimethalin |
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Citations (9)
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| CN207571147U (en) * | 2017-09-30 | 2018-07-03 | 山东爱维德生物科技有限公司 | For the test strips of fluorescence immune chromatography method detection |
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| CN109061147A (en) * | 2018-09-21 | 2018-12-21 | 中国烟草总公司郑州烟草研究院 | A kind of test strips and its preparation method and application detecting pendimethalin |
| CN109061149A (en) * | 2018-09-21 | 2018-12-21 | 中国烟草总公司郑州烟草研究院 | A kind of time-resolved fluoroimmunoassay chromatograph test strip and its preparation method and application detecting butralin |
| CN109061156A (en) * | 2018-09-21 | 2018-12-21 | 中国烟草总公司郑州烟草研究院 | A kind of time-resolved fluoroimmunoassay chromatograph test strip and its preparation method and application detecting pendimethalin |
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| CN208808842U (en) * | 2017-12-11 | 2019-05-03 | 杭州有尚贸易有限公司 | The sterile spill-preventive breast pad of water lock |
| CN111122856A (en) * | 2020-01-10 | 2020-05-08 | 中国农业科学院烟草研究所 | Time-resolved fluorescence immunochromatography test paper card for detecting butralin |
-
2020
- 2020-05-27 CN CN202010462269.7A patent/CN111579790B/en active Active
- 2020-12-18 NZ NZ771345A patent/NZ771345A/en unknown
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207571147U (en) * | 2017-09-30 | 2018-07-03 | 山东爱维德生物科技有限公司 | For the test strips of fluorescence immune chromatography method detection |
| CN207579273U (en) * | 2017-12-07 | 2018-07-06 | 苏州吉臣日用品有限公司 | A kind of blotting paper |
| CN208808842U (en) * | 2017-12-11 | 2019-05-03 | 杭州有尚贸易有限公司 | The sterile spill-preventive breast pad of water lock |
| CN108398557A (en) * | 2018-02-09 | 2018-08-14 | 河南省生物工程技术研究中心有限公司 | A kind of myeloperoxidase fluorescence immune chromatography test paper bar and test card |
| CN109061147A (en) * | 2018-09-21 | 2018-12-21 | 中国烟草总公司郑州烟草研究院 | A kind of test strips and its preparation method and application detecting pendimethalin |
| CN109061149A (en) * | 2018-09-21 | 2018-12-21 | 中国烟草总公司郑州烟草研究院 | A kind of time-resolved fluoroimmunoassay chromatograph test strip and its preparation method and application detecting butralin |
| CN109061156A (en) * | 2018-09-21 | 2018-12-21 | 中国烟草总公司郑州烟草研究院 | A kind of time-resolved fluoroimmunoassay chromatograph test strip and its preparation method and application detecting pendimethalin |
| CN109324182A (en) * | 2018-09-21 | 2019-02-12 | 中国烟草总公司郑州烟草研究院 | A kind of fluorescent micro-ball immune chromatography test paper strip and its preparation method and application detecting pendimethalin |
| CN111122856A (en) * | 2020-01-10 | 2020-05-08 | 中国农业科学院烟草研究所 | Time-resolved fluorescence immunochromatography test paper card for detecting butralin |
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| Publication number | Publication date |
|---|---|
| NZ771345A (en) | 2022-09-30 |
| CN111579790B (en) | 2023-08-04 |
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